Alternating tangential flow perfusion system

Abstract

The utility model relates to an alternating tangential flow perfusion system and a control method thereof, and the alternating tangential flow perfusion system comprises a consumable component, a weighing component, an air inflation component, an air exhaust component and a control box, the consumable assembly is arranged on the weighing assembly, the weighing assembly is used for monitoring the weight of fluid in the consumable assembly in real time, the control box judges the motion state of the elastic balloon according to the variable quantity of the fluid weight and the change rate of the fluid weight, and then the working states of the air inflation assembly and the air exhaust assembly are controlled; according to the alternating tangential flow perfusion system and the control method thereof, the defects in the prior art are overcome, and system operation is more stable and reliable.

Description

Technical Field

[0001] The utility model relates to an alternating tangential flow perfusion system. Background Art

[0002] In recent years, the biopharmaceutical industry often uses an alternating tangential flow perfusion system to obtain high-density cell culture results. The alternating tangential flow perfusion system uses an alternating method to push the culture solution through the filter membrane, which has the advantages of good membrane filament flushing effect, low shear force, and high working efficiency. Generally, the alternating tangential flow perfusion system can generate variable power by jointly driving the diaphragm by vacuum and compressed air. The diaphragm pushes the culture medium to flow back and forth for hollow fiber flushing. During the driving process, the pressure sensor detects the pressure change of the driving diaphragm to judge whether the diaphragm reaches the limit position. Once the pressure sensor detects a sudden pressure change, it is considered that the diaphragm has reached the limit position, and the control system executes a switching action to switch the flushing direction.

[0003] However, it is found in the actual use process that the existing alternating tangential flow perfusion system is not stable and often has misoperations. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an alternating tangential flow perfusion system to overcome the existing defects, so that the system runs more stably and reliably.

[0005] The technical solution to achieve the above purpose is: an alternating tangential flow perfusion system, including: a consumable component, a weighing component, an air inflation component, an air extraction component, and a control box;

[0006] The consumable component includes a rigid transparent spherical shell, an elastic balloon, and a hollow fiber column. The elastic balloon is arranged inside the rigid transparent spherical shell. The inner cavity of the elastic balloon is communicated with the hollow fiber column connected to the upper end of the rigid transparent spherical shell. The upper end connection port of the hollow fiber column is communicated with the reactor through a first infusion pipeline, and the upper end drain port of the hollow fiber column is communicated with the waste liquid bottle through a second infusion pipeline;

[0007] The consumable component is arranged on the weighing component, and the weighing component is used for real-time monitoring of the fluid weight inside the consumable component;

[0008] The weighing component, the air inflation component, and the air extraction component are all connected to the control box. The air inflation component is used for inflating between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon shrinks under the action of the pressure difference. The air extraction component is used for extracting air between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon expands under the action of the pressure difference. The control box judges the motion state of the elastic balloon according to the change amount and change rate of the fluid weight, and then controls the working states of the air inflation component and the air extraction component.

[0009] Preferably, the control box includes a gas path control module which has an inflation path and an air extraction path. An inflation port, a precision pressure regulating valve, a pressure ratio valve, a gas flow meter and a pneumatic control solenoid valve are sequentially arranged on the inflation path. An air extraction port, a vacuum control valve, a pressure ratio valve, a gas flow meter and a pneumatic control solenoid valve are sequentially arranged on the air extraction path.

[0010] Preferably, the inflation assembly includes a compressed air source and a compressed air connection pipeline. One end of the compressed air connection pipeline is connected to the compressed air source, and the other end is connected to the inflation port of the control box. The gas path output end of the control box is connected to the consumable component through a third connection pipeline.

[0011] Preferably, the air extraction assembly includes a vacuum pump and a second connection pipeline. One end of the second connection pipeline is connected to the vacuum pump, and the other end is connected to the air extraction port of the control box.

[0012] Preferably, the consumable component is further connected to a bracket assembly. The lower end of the bracket assembly is fixed on the weighing assembly, and the bracket assembly is used to support the consumable component.

[0013] Preferably, a first peristaltic pump is connected to the second infusion pipeline, and the reactor is communicated with a replenishing solution bottle through a third infusion pipeline. A second peristaltic pump is connected to the third infusion pipeline.

[0014] The beneficial effects of the present utility model are as follows: For the present alternating tangential flow perfusion system and its control method, it can solve the problem that when the existing alternating tangential flow perfusion system operates, after detecting a sudden pressure change through a pressure sensor and then controlling the system to perform a switching action, since air can be compressed and the input pressure of the gas will also change with the input flow rate, there will often be a situation where the sudden pressure change does not occur after reaching the theoretical switching point, or the sudden pressure change occurs in advance, resulting in inconsistent lengths of time for the control system to drive the balloon to move and the system being unstable. At the same time, since the switching logic used monitors the weight derivative as the main switching condition, it can effectively solve the problem that during the use of the equipment, due to uncontrollable situations, the weight drifts, thereby affecting the operation of the equipment.

[0015] In addition, the present device takes the combination of weight change and weight change rate as the switching condition, rather than taking weight as the sole switching condition, which can effectively avoid the situation where the elastic balloon or the diaphragm of the diaphragm pump does not move to the limit position, and the weight cannot reach the peak or valley value, thus affecting the continuous operation of the equipment. Description of the Drawings

[0016] Figure 1 is a flowchart of the weight switching logic method of the alternating tangential flow perfusion system of the present utility model;

[0017] Figure 2 is a schematic structural diagram of the alternating tangential flow perfusion system of the present utility model;

[0018] Figure 3 is the weight and pressure change curve of the alternating tangential flow perfusion system of the present utility model during operation;

[0019] Figure 4 is a schematic diagram of the movement of the elastic balloon of the alternating tangential flow perfusion system of the present utility model;

[0020] Figure 5 is a schematic diagram of the control method of the alternating tangential flow perfusion system of the present utility model;

[0021] Figure 6 is a schematic diagram of the gas path control module of the alternating tangential flow perfusion system of the present utility model.

[0022] In the figure: 1. Consumable component; 2. Bracket component; 3. Weighing component; 4. Control box; 5. Vacuum pump; 6. Compressed air connection pipeline; 7. Second connection pipeline; 8. Third connection pipeline; 9. First peristaltic pump; 10. Reactor; 11. First infusion pipeline; 12. Second infusion pipeline; 13. Third infusion pipeline; 14. Waste liquid bottle; 15. Supplementary liquid bottle; 16. Second peristaltic pump. Specific embodiments

[0023] Next, the technical solutions of the present utility model will be clearly and completely described with reference to the accompanying drawings. In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0024] Next, the present utility model will be further described with reference to the accompanying drawings.

[0025] Such as Figure 1-4As shown in the figure, the alternating tangential flow perfusion system includes: a consumable component 1, a weighing component 3, an inflation component, a gas extraction component, and a control box 4; the consumable component 1 includes a rigid transparent spherical shell, an elastic balloon, and a hollow fiber column. The elastic balloon is arranged inside the rigid transparent spherical shell. The inner cavity of the elastic balloon communicates with the hollow fiber column connected to the upper end of the rigid transparent spherical shell. The upper docking port of the hollow fiber column is connected to the reactor 10 through a first infusion pipeline 11, and the upper liquid discharge port of the hollow fiber column is connected to the waste liquid bottle 14 through a second infusion pipeline 12; the consumable component 1 is connected to the weighing component 3, and the weighing component 3 is used to monitor the fluid weight inside the consumable component 1 in real time.

[0026] Specifically, the weighing component 3, the inflation component, and the gas extraction component are all connected to the control box 4. The inflation component is used to inflate between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon shrinks under the action of the pressure difference. The gas extraction component is used to extract gas from between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon expands under the action of the pressure difference. The control box 4 judges the motion state of the elastic balloon according to the change of the fluid weight monitored by the weighing component 3 and the change rate of the fluid weight, and then controls the working states of the inflation component and the gas extraction component.

[0027] As Figure 6 shown in the figure, the control box 4 includes a gas path control module. The gas path control module has an inflation path and an extraction path. An inflation port, a precision pressure regulating valve, a pressure proportional valve, a gas flow meter, and a pneumatic control solenoid valve are sequentially arranged on the inflation path. A gas extraction port, a vacuum control valve, a pressure proportional valve, a gas flow meter, and a pneumatic control solenoid valve are sequentially arranged on the extraction path.

[0028] Specifically, the inflation component includes a compressed air source and a compressed air connection pipeline 6. One end of the compressed air connection pipeline 6 is connected to the compressed air source, and the other end of the compressed air connection pipeline 6 is connected to the inflation port of the control box 4. The gas path output end of the control box 4 is connected to the consumable component 1 through a third connection pipeline 8.

[0029] Specifically, the space between the elastic balloon and the rigid transparent spherical shell is communicated with the gas path output end of the control box 4 through the third connection pipeline 8. A ventilation hole can be opened on the rigid transparent spherical shell or the connecting pipe below it, and the third connection pipeline 8 is hermetically docked with the ventilation hole.

[0030] Specifically, the gas extraction component includes a vacuum pump 5 and a second connection pipeline 7. One end of the second connection pipeline 7 is connected to the vacuum pump 5, and the other end of the second connection pipeline 7 is connected to the gas extraction port of the control box 4.

[0031] Specifically, the consumable component 1 is also connected with a support component 2. The lower end of the support component 2 is fixed on the weighing component 3, and the support component 2 is used to support the consumable component 1.

[0032] Specifically, a first peristaltic pump 9 is connected to the second infusion pipeline 12. The reactor 10 communicates with the replenishing bottle 15 through a third infusion pipeline 13, and a second peristaltic pump 16 is connected to the third infusion pipeline 13. The controls of the first peristaltic pump 9 and the second peristaltic pump 16 can be set independently, integrated with the electronic control unit of the reactor, or integrated into the control box 4. The first peristaltic pump 9 is used to extract waste liquid, and the second peristaltic pump 16 is used to supplement new liquid. Generally, the extraction amount of the waste liquid is kept consistent with the supplement amount of the new liquid to ensure that the liquid quality in the reactor remains unchanged.

[0033] As Figure 2 shown, the elastic balloon inside the consumable component 1 is controlled by the control box 4 to pulsate periodically, thereby controlling the periodic exchange of liquid (i.e., the culture medium) inside the hollow fiber column and inside the reactor 10. At the same time, the first peristaltic pump 9 is continuously used to extract the used culture medium intercepted by the hollow fiber column from inside the hollow fiber column, and at the same time, the second peristaltic pump 16 is used to pump the fresh culture medium in the replenishing bottle into the reactor 10 to achieve the purpose of exchanging the culture medium inside the reactor.

[0034] During the operation, the control box 4 will calculate the change rate of the weight based on the liquid weight monitored by the weighing component 3 in real time, obtain the change curves of the weight and the weight derivative respectively, and judge whether the expansion or contraction of the elastic balloon reaches the limit position according to the preset change amount parameter and change rate parameter, and then decide whether to perform a switching operation.

[0035] As Figure 3 shown, in one action cycle, when the sphere in the consumable component 1 is completely deflated, the liquid weight of the consumable component 1 and its pipeline is at the lowest value at this time, denoted as W1, and the air extraction action is performed. The elastic balloon starts to expand under the pressure difference, and the liquid flows into the elastic balloon. The liquid weight of the consumable component 1 and its pipeline gradually rises. When the change amount of the weight is greater than or equal to the first set value (i.e., the real-time weight - W1 ≥ △W), the change rate of the liquid weight is compared with the second set value. If the change rate of the weight is less than or equal to the second set value, it is considered that the sphere has been fully expanded. At this time, the liquid weight of the consumable component 1 and its pipeline is at the maximum value, denoted as W2. Then, the air inflation action is switched, and the elastic balloon starts to contract under the pressure difference, and the liquid flows out of the elastic balloon. The liquid weight of the consumable component 1 and its pipeline gradually decreases. When the change amount of the liquid weight is greater than or equal to the third set value (i.e., W2 - the real-time weight ≥ △W), the change rate of the liquid weight is compared with the fourth set value. If the change rate of the liquid weight is less than or equal to the fourth set value, it is considered that the sphere has been completely deflated, and the air extraction action is switched again.

[0036] Among them, both the first set value and the third set value are change amount parameters of the liquid weight, and both the second set value and the fourth set value are change rate parameters of the liquid weight.

[0037] In this embodiment, the first set value is equal to the third set value, both being ΔW. ΔW can be set according to the maximum weight W2 of the fluid in the consumable component 1, and is usually set to 60%-80% of the maximum weight W2. For example, if the maximum weight W2 is 100 grams, ΔW can be set to 80% of the maximum weight, that is, 80 grams. Thus, when the fluid weight decreases from 100 grams to 20 grams, the change rate of the fluid weight starts to be calculated.

[0038] In this embodiment, the second set value is equal to the fourth set value, both being ΔV. ΔV can be set according to the measurement error of the fluid weight change rate, and the measurement error comes from the measurement error of the weighing component, or when the elastic balloon moves at a high speed, the consumable component 1 shakes. For example, ΔV is set to ±0.1 g / s. When the change rate of the fluid weight decreases to 0.1 g / s, it can be considered that the fluid weight no longer changes and the switching condition is reached.

[0039] In addition, the control box 4 also compares the action time with the theoretical time to determine whether the elastic balloon moves too fast or too slow, thereby adjusting the driving force of the device, reducing or increasing the driving force, and thus controlling the movement speed of the liquid inside the hollow fiber. During each action process, if the switching condition is not reached for a long time, forced switching will be performed according to the action timeout logic.

[0040] The alternating tangential flow perfusion system provided in this embodiment can solve the problem that in the existing alternating tangential flow perfusion system, when operating, the pressure sensor detects a sudden pressure change and then the control system performs a switching action. Because air can be compressed and the input pressure of the gas also changes with the input flow rate, it often occurs that after reaching the theoretical switching point, the sudden pressure change does not occur, or the sudden pressure change occurs in advance, resulting in different lengths of time for the control system to drive the diaphragm to move and the system being unstable. At the same time, since the switching logic used mainly monitors the weight derivative as the switching condition, it can effectively solve the problem that during the use of the device, the weight drifts due to uncontrollable situations, thereby affecting the operation of the device.

[0041] In addition, the device adopts the combination of weight change and weight change rate as the switching condition, rather than using weight as the sole switching condition, which can effectively avoid the situation where sometimes the elastic balloon does not move to the extreme position and the weight cannot reach the peak or valley value, thus affecting the continuous operation of the device.

[0042] Correspondingly, this embodiment also provides a control method for an alternating tangential flow perfusion system. As Figure 5 shown, the control method for the alternating tangential flow perfusion system includes the following steps:

[0043] Step S1, inflate the rigid transparent spherical shell through the inflation component to completely contract the elastic balloon;

[0044] Step S2: Evacuate the inside of the rigid transparent spherical shell through the air extraction component to fully inflate the elastic balloon.

[0045] Step S3: Continuously loop through Steps S1 - S2 until the cell perfusion culture process is completed.

[0046] Specifically, Step S1 includes:

[0047] Step S11: Initially, the elastic balloon is in a fully inflated state. The control box 4 controls the compressed air connection pipeline 6 to inflate the inside of the rigid transparent spherical shell, causing the elastic balloon to gradually contract. During this process, the weighing component 3 monitors the fluid weight in the consumable component 1 in real time.

[0048] When the change in fluid weight is greater than or equal to the first set value, calculate the change rate of the fluid weight. If the change rate of the fluid weight is less than or equal to the second set value, it is considered that the elastic balloon has been fully deflated and the switching condition is reached.

[0049] Step S12: Close the compressed air connection pipeline 6 and record the actual operation time of the inflation operation.

[0050] After completing this inflation operation and before the next inflation operation, it also includes: comparing the actual operation time of the inflation operation with the theoretical operation time.

[0051] If the actual operation time is greater than the theoretical operation time, the control box 4 increases the driving force of the next inflation operation through the gas path control module. If the actual operation time is less than the theoretical operation time, the control box 4 reduces the driving force of the next inflation operation through the gas path control module.

[0052] Step S2 includes:

[0053] Step S21: The control box 4 controls the vacuum pump 5 to evacuate the inside of the rigid transparent spherical shell, causing the elastic balloon to gradually expand. During this process, the weighing component 3 monitors the fluid weight in the consumable component 1 in real time.

[0054] When the change in fluid weight is greater than or equal to the third set value, calculate the change rate of the fluid weight. If the change rate of the fluid weight is less than or equal to the fourth set value, it is considered that the elastic balloon has been fully inflated and the switching condition is reached.

[0055] Step S22: Close the vacuum pump 5 and record the actual operation time of the evacuation operation.

[0056] After completing this evacuation operation and before the next evacuation operation, it also includes: comparing the actual operation time of the evacuation operation with the theoretical operation time.

[0057] If the actual operation time is greater than the theoretical operation time, the control box 4 increases the driving force for the next air extraction operation through the air circuit control module; if the actual operation time is less than the theoretical operation time, the control box 4 reduces the driving force for the next air extraction operation through the air circuit control module.

[0058] Among them, the theoretical operation time is set according to the time requirement of the cell perfusion culture process. If the cell perfusion culture process requires that the time period for each air inflation operation is t1, then the theoretical operation time for the air inflation operation is t1. Similarly, if the cell perfusion culture process requires that the time period for each air extraction operation is t2, then the theoretical operation time for the air extraction operation is t2. The theoretical operation time t2 for each air extraction operation can be equal to the theoretical operation time t1 for the air inflation operation.

[0059] During the cyclic switching process between the air inflation operation and the air extraction operation, if the actual operation time exceeds the preset time and the switching condition is still not met, a forced switching is performed according to the action timeout logic.

[0060] If the theoretical operation time for each air inflation operation and each air extraction operation is t, the preset time can be set between 1.1t and 1.3t. For example, if the preset time is 1.2t and the actual operation time of the air inflation operation or the air extraction operation reaches 1.2t but the switching condition is still not met, the driving force is increased to directly perform a forced switching.

[0061] The alternating tangential flow perfusion system and its control method provided by this application detect how much liquid enters the pipeline of the consumable component 1 (including the hollow fiber column and the elastic balloon) by placing a weighing component 3 at the bottom of the consumable component 1 to weigh the consumable component 1 in real time. During the movement of the elastic balloon, the liquid weight in the pipeline of the consumable component 1 changes in real time. When the elastic balloon moves to the extreme position, the liquid in the pipeline of the consumable component 1 basically stops flowing, the flow rate approaches zero, and the weight of the consumable component 1 no longer changes.

[0062] In the consumable component 1, the internal volume of the hollow fiber column is fixed, and the volume of the elastic balloon can vary between a maximum volume and a minimum volume. Therefore, the volume change of the elastic balloon can be judged by the amplitude of the weight change (for example: during the movement of the elastic balloon, the hollow fiber column always remains full. If the volume of the elastic balloon when fully inflated is X, and when it is completely deflated, the volume of the balloon becomes 0. At this time, X volume of liquid enters the hollow fiber column. At the same time, since the volume of the hollow fiber column is fixed and was also full before the X volume entered, when these X volume of liquid enters, it will cause the X volume of liquid originally in the hollow fiber column to be spit out. Since the hollow fiber is connected to the reactor, these spit-out liquids enter the reactor. During the entire operation process, the total volume of the liquid inside the hollow fiber column remains unchanged, and what changes is the volume of the liquid inside the elastic balloon). Therefore, when the amplitude of the weight change of the consumable component 1 does not reach the set value, it means that the elastic balloon is not fully inflated or not fully contracted.

[0063] The utility model inventors have found that in the existing alternating tangential flow perfusion system, after detecting a sudden change in the diaphragm pressure through a pressure sensor, the switching action is executed. The premise for this control method to be realized is that the position of the diaphragm does not move after reaching the limit position (contacting the upper spherical shell or the lower spherical shell), and the compressed air or vacuum still continuously outputs power to the diaphragm to form a sudden change in pressure. However, the sudden change in pressure only reflects that the diaphragm has reached the limit position, and has nothing to do with whether there is liquid in the sphere and whether the volume of the liquid changes. If an abnormal situation occurs when the control system drives the diaphragm to move and suck in liquid, and there is actually no liquid in the sphere, a sudden change in pressure will still occur when the diaphragm reaches the limit position. In this way, the control system will make a wrong judgment, thinking that the switching condition has been reached and spit out the liquid in the reverse direction, resulting in misoperation. Therefore, this control method can only ensure that the driving force of the diaphragm is relatively regular, and cannot judge whether the situation of liquid inhalation and spitting is consistent with the movement of the diaphragm. Just measuring the change in air pressure, the result is unreliable. Moreover, since air can be compressed, the input pressure of the gas will also change with the change in the input flow rate. Therefore, there often occurs a situation where the sudden change in pressure does not occur after reaching the theoretical switching point, or the sudden change in pressure occurs in advance. In this way, it leads to the problem that the time for the control system to drive the diaphragm to move is different, and the system runs unstably.

[0064] To solve the above problems, the present application monitors the weight change of the consumable component in real time (actually mainly the weight change of the liquid inside it), and judges whether the elastic balloon of the consumable component reaches the limit position according to whether the weight reaches the peak value and the valley value.

[0065] It is considered that the measurement deviation and drift of the weighing component may cause misjudgment. For example, when the elastic balloon is contracting, if the scale deviates and drifts, the measured value will not be able to reach the predetermined valley value or will reach the predetermined valley value too early, thereby affecting the normal operation of the device. When the elastic balloon is expanding, if the scale deviates or drifts, the measured value will not be able to reach the predetermined peak value or will reach the predetermined peak value too early, thereby affecting the normal operation of the device. In addition, when the liquid in the elastic balloon reaches the limit position during movement, there will be a certain inertial impact. Although the total amount of liquid no longer changes, the inertia of movement will cause the peak weight measured by the weighing component to be too large and the valley weight to be too small. This will cause the liquid to not be drained or not full, but the system judges that the switching condition has been reached. For this reason, the present application not only uses the change in weight as a judgment indicator, but also uses the rate of change of weight as another judgment indicator, and combines these two indicators to judge whether the movement of the elastic balloon has reached the limit state.

[0066] Specifically, when the elastic balloon is completely compressed or fully expanded, the rate of change of the liquid weight will decrease. When the rate of change of the weight is close to zero, it means that the amount of liquid in the elastic balloon no longer changes. By combining the weight change amount and the weight change rate as the switching condition, the measurement deviation caused by weighing drift and liquid inertial impact can be effectively avoided, thereby ensuring that the liquid inside the elastic balloon can be drained or filled during the switching action, thereby ensuring that the alternating tangential flow control system is more stable and reliable.

[0067] The above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model is described in detail with reference to the above embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the above embodiments, or replace some or all of the technical features therein with equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model.

Claims

1. An alternating tangential flow perfusion system, characterized in that, Comprising: a consumable component (1), a weighing component (3), an inflation component, a gas extraction component, and a control box (4); The consumable component (1) includes a rigid transparent spherical shell, an elastic balloon, and a hollow fiber column. The elastic balloon is disposed inside the rigid transparent spherical shell. The inner cavity of the elastic balloon communicates with the hollow fiber column connected to the upper end of the rigid transparent spherical shell. The upper end docking port of the hollow fiber column communicates with a reactor (10) through a first infusion pipeline (11), and the upper end drain port of the hollow fiber column communicates with a waste liquid bottle (14) through a second infusion pipeline (12); The consumable component (1) is disposed on the weighing component (3), and the weighing component (3) is used to monitor the fluid weight inside the consumable component (1) in real time; The weighing component (3), the inflation component, and the gas extraction component are all connected to the control box (4). The inflation component is used to inflate between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon contracts under the action of the pressure difference. The gas extraction component is used to extract gas between the elastic balloon and the rigid transparent spherical shell, so that the elastic balloon expands under the action of the pressure difference. The control box (4) determines the motion state of the elastic balloon according to the change amount and change rate of the fluid weight, and then controls the working states of the inflation component and the gas extraction component.

2. The alternating tangential flow perfusion system according to claim 1, wherein The control box (4) includes a gas path control module. The gas path control module has an inflation path and a gas extraction path. An inflation port, a precision pressure regulating valve, a pressure proportional valve, a gas flow meter, and a pneumatic control solenoid valve are sequentially arranged on the inflation path. A gas extraction port, a vacuum control valve, a pressure proportional valve, a gas flow meter, and a pneumatic control solenoid valve are sequentially arranged on the gas extraction path.

3. The alternating tangential flow perfusion system according to claim 2, wherein The inflation component includes a compressed air source and a compressed air connection pipeline (6). One end of the compressed air connection pipeline (6) is connected to the compressed air source, and the other end of the compressed air connection pipeline (6) is connected to the inflation port of the control box (4). The gas path output end of the control box (4) is connected to the consumable component (1) through a third connection pipeline (8).

4. The alternating tangential flow perfusion system according to claim 2, wherein The gas extraction component includes a vacuum pump (5) and a second connection pipeline (7). One end of the second connection pipeline (7) is connected to the vacuum pump (5), and the other end of the second connection pipeline (7) is connected to the gas extraction port of the control box (4).

5. The alternating tangential flow perfusion system according to claim 1, wherein The consumable component (1) is further connected to a support component (2). The lower end of the support component (2) is fixed on the weighing component (3), and the support component (2) is used to support the consumable component (1).

6. The alternating tangential flow perfusion system according to claim 1, wherein A first peristaltic pump (9) is connected to the second infusion pipeline (12). The reactor (10) communicates with a replenishing liquid bottle (15) through a third infusion pipeline (13), and a second peristaltic pump (16) is connected to the third infusion pipeline (13).

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