Multi-chamber cell culture bag
The multi-chamber cell culture bag is connected by sealed chamber design and hose clips, solving the temperature fluctuations and contamination problems caused by folding the cell culture bag and adding multiple liquids, achieving high-efficiency cell culture with stable temperature and pollution-free temperature.
Patent Information
- Application Number
- CN202422208663.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-10
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-09-10
AI Technical Summary
The cell culture bag needs to be folded manually. The multiple addition of culture medium causes the cell culture ambient temperature to drop sharply repeatedly, and the open device is prone to contamination, affecting cell growth and proliferation.
A multi-chamber cell culture bag is designed to separate the bag body into chambers A, B, and C through sealing strips, and connected with hoses and clips to achieve the addition of cell suspensions without folding and temperature control, avoiding open device operation.
It realizes stable temperature control and reduced pollution risk during cell culture, improves cell growth and proliferation efficiency, and simplifies the operation process.
Smart Images
Figure CN223150571U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of cell culture devices, in particular to a multi-chamber cell culture bag, which is suitable for culturing immune cells such as CAR-T, NK, and CIK, and can realize the growth and proliferation of immune cells in a sealed environment. Background Technique
[0002] During the culture process of immune cells such as CAR-T, NK, and CIK cells, the cell suspension is usually loaded into a culture bag and incubated in a sealed environment to achieve the survival and growth of the cells. This cell culture technology has been widely used in the fields of biology, medicine, new drug research and development, etc.
[0003] Immune cells are usually initially cultured in a cell culture flask. When the cultured cells expand to a certain number (generally, the volume of the cell suspension is about 200 ml), the culture flask cannot hold them, and because the cell culture bag has a large gas exchange area and is more conducive to cell growth, it is necessary to transfer them to the cell culture bag for continued culture. After transferring to the cell culture bag, due to the small total amount of the cell suspension in the early stage of cell culture and the large volume of the cell culture bag, immune cells need to aggregate to grow and proliferate better. Therefore, the cell culture bag needs to be manually folded to make the cell suspension gather, but the folded culture bag often bounces open due to elastic action, causing the cell suspension to disperse and the cells to be unable to aggregate and proliferate.
[0004] During the culture process, according to the adjustment of cell density, it is necessary to take out the cell culture bag from the 37°C incubator multiple times and put it into a biosafety cabinet or a clean bench to add culture medium, which will cause the temperature of the cell suspension to drop sharply. Especially in winter, it will have a great impact on NK cells, CAR-T cells, and CAR-NK cells with high requirements for growth conditions, resulting in a slowdown in the proliferation rate. In addition, adding culture medium multiple times requires opening the cell culture device, which is extremely easy to be contaminated. Therefore, a multi-chamber cell culture bag is needed to solve the above problems. Summary of the Utility Model
[0005] The purpose of the utility model is to provide a multi-chamber cell culture bag to solve the problems in the above background technique that the cell culture bag needs to be manually folded, adding culture medium multiple times causes the temperature of the cell culture environment to drop repeatedly and sharply, and opening the cell culture device for adding culture medium multiple times is extremely easy to be contaminated.
[0006] To achieve the above purpose, the utility model provides the following technical solutions:
[0007] A multi-chamber cell culture bag, comprising a cell culture bag body, a sampling port, an injection port, a first chamber connecting hose, a second chamber connecting hose, a first hose clamp, and a second hose clamp; the cell culture bag body is divided into chamber A, chamber B, and chamber C by a sealing strip; the injection port is connected to an injection liquid hose, and the other end of the injection liquid hose is provided with a syringe filling port and a threaded cap; the sampling port is located on the right side of the injection port.
[0008] As a preferred technical solution of the present invention, the first chamber connecting hose connects chamber A and chamber B, and the second chamber connecting hose connects chamber B and chamber C.
[0009] As a preferred technical solution of the present invention, both the first hose clamp and the second hose clamp are Robert clamps, and the first hose clamp and the second hose clamp have two states: sealed and opened.
[0010] As a preferred technical solution of the present invention, there is a liquid level scale line outside chamber A of the cell culture bag body, with a scale of 0 - 200 ml, there is a liquid level scale line outside chamber B of the cell culture bag body, with a scale of 0 - 500 ml, and there is a liquid level scale line outside chamber C of the cell culture bag body, with a scale of 0 - 700 ml.
[0011] As a preferred technical solution of the present invention, the first chamber connecting hose, the second chamber connecting hose, and the injection liquid hose are made of PP material, and the cell culture bag body is made of polyethylene or polypropylene.
[0012] As a preferred technical solution of the present invention, the caliber of the syringe filling port matches the size of the interface of the 50 ml syringe barrel.
[0013] As a preferred technical solution of the present invention, the end interface of the syringe filling port is provided with a thread, and the size of the thread matches the thread size of the threaded cap. Description of the Drawings
[0014] Figure 1 It is a schematic diagram of the multi-chamber cell culture bag provided by the embodiment of the present invention.
[0015] In the figure: 1 - culture bag body, 2 - sampling port, 3 - injection port, 401 - first chamber connecting hose, 402 - second chamber connecting hose, 501 - first hose clamp, 502 - second hose clamp, 6 - chamber A, 7 - chamber B, 8 - chamber C, 9 - injection liquid hose, 10 - syringe filling port, 11 - threaded cap. Detailed Embodiments
[0016] In order to make the technical means, creative features, achieved purposes and functions realized by the present utility model easy to understand, the present utility model will be further described below in conjunction with specific embodiments. Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without creative labor all fall within the protection scope of the present utility model.
[0017] It should be noted that: Similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.
[0018] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the utility model product is usually placed during use. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation to the present utility model. In addition, the terms "first", "second", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.
[0019] The present utility model will be further described below in conjunction with the drawings and specific embodiments.
[0020] A multi-chamber cell culture bag, as Figure 1 , includes a cell culture bag body 1, a sampling port 2, an injection port 3, a first chamber connecting hose 401, a second chamber connecting hose 402, a first hose clamp 501, and a second hose clamp 502. The cell culture bag body 1 is divided into chamber A 6, chamber B 7, and chamber C 8 by a sealing strip. The injection port 3 is connected to an injection liquid hose 9, and the other end of the injection liquid hose 9 is provided with a syringe filling port 10 and a screw cap 11. The sampling port 2 is located on the right side of the injection port 3.
[0021] In the specific use process, first open the first hose clamp 501 and the second hose clamp 502, and inject all the culture media required for the entire cell culture cycle into the injection liquid hose 9 through the syringe filling port 10. The culture media will flow into chambers B and C respectively through the first chamber connecting hose 401 and the second chamber connecting hose 402, and then close the first hose clamp 501 and the second hose clamp 502. At this time, chambers B and C are in a closed and sealed state.
[0022] Inject 200 ml of the cell suspension into chamber A6 through the syringe filling port 10 of the injection hose 9. Cover the syringe filling port 10 with the screw cap 11 and rotate it tightly to seal. Since the volume of chamber A6 is 200 ml, which is in line with the total amount of the cell suspension transferred from the cell culture flask to the cell culture bag, there is no need to fold the cell culture bag. Just lay the cell culture bag flat and place it in the incubator.
[0023] When the number of cells cultured in chamber A reaches a certain amount, only need to open the first hose clamp 501 connecting chamber A6 and chamber B7 in the incubator, then the cell suspension in chamber A6 can be automatically mixed with the pre-added culture medium at the same temperature in chamber B7. The operation is fast and does not affect the temperature of the cell culture environment. The whole operation of adding the culture medium does not open the cell culture device, avoiding the contact between the cell culture device and the external environment and reducing the possibility of being contaminated.
[0024] When the number of cells cultured in chambers A and B reaches a certain amount, only need to open the second hose clamp 502 connecting chamber B7 and chamber C8 in the incubator, then the cell suspensions in chambers A6 and B7 can be automatically mixed with the pre-added culture medium at the same temperature in chamber C8. The operation is fast and does not affect the temperature of the cell culture environment. The whole operation of adding the culture medium does not open the cell culture device, avoiding the contact between the cell culture device and the external environment and reducing the possibility of being contaminated.
[0025] During the culture process, samples can be collected at any time through the sampling port 2 located on the right side of the injection port 3 to detect the growth state of the cells.
[0026] The bag body 1 of the cell culture bag is made of breathable polyethylene or polypropylene material, and the first chamber connecting hose 401, the second chamber connecting hose 402 and the injection hose 9 are made of PP material, ensuring the biocompatibility and chemical stability of the whole culture bag.
[0027] This multi-chamber cell culture bag is easy to use and flexible in operation. Chambers A6, B7 and C8 are respectively used for cell culture at different stages. The independence and sealing of each chamber are ensured by the sealing strip, which is suitable for the cell culture needs at different stages. Through reasonable distribution of the culture medium and sample collection, efficient and precise cell culture operations can be achieved.
Claims
1. A multi-chamber cell culture bag, comprising a cell culture bag body (1), a sampling port (2), an injection port (3), a first chamber connecting hose (401), a second chamber connecting hose (402), a first hose clamp (501), and a second hose clamp (502), characterized in that: The cell culture bag body (1) is separated into chamber A (6), chamber B (7), and chamber C (8) by a sealing strip; The injection port (3) is connected to an injection hose (9), and the other end of the injection hose (9) is provided with a syringe filling port (10) and a threaded cap (11); the sampling port (2) is located on the right side of the injection port (3).
2. The multi-chamber cell culture bag according to claim 1, characterized in that, The first chamber connecting hose (401) connects chamber A (6) and chamber B (7), and the second chamber connecting hose (402) connects chamber B (7) and chamber C (8).
3. The multi-chamber cell culture bag according to claim 1, characterized in that, Both the first hose clamp (501) and the second hose clamp (502) are Robert clamps, and the first hose clamp (501) and the second hose clamp (502) have two states: sealed and open.
4. A multi-chamber cell culture bag according to claim 1, wherein There is a liquid level scale line outside chamber A (6) of the cell culture bag body (1), with a scale of 0 - 200 ml; there is a liquid level scale line outside chamber B (7) of the cell culture bag body (1), with a scale of 0 - 500 ml; there is a liquid level scale line outside chamber C (8) of the cell culture bag body (1), with a scale of 0 - 700 ml.
5. The multi-chamber cell culture bag according to claim 1, characterized in that, The first chamber connecting hose (401), the second chamber connecting hose (402), and the injection hose (9) are made of PP material, and the cell culture bag body (1) is made of polyethylene or polypropylene.
6. The multi-chamber cell culture bag according to claim 1, wherein, The caliber of the syringe filling port (10) matches the size of the interface of a 50 ml syringe barrel.
7. A multi-chamber cell culture bag according to claim 1, characterized in that, The end interface of the syringe filling port (10) is provided with threads, and the thread size matches the thread size of the threaded cap (11).