Embryo vitrification freezing carrying rod

Abstract

The utility model relates to an embryo vitrification freezing carrying rod which comprises a body, a liquid loading area is arranged on the body, and a sample loading area is arranged in the liquid loading area. The utility model discloses an anti-theft bolt, which comprises a body, and further comprises a stud, one end of the body is provided with a clamping head, the stud is provided with a clamping groove, the clamping head is arranged in the clamping groove, and the body is clamped on the stud through the matching of the clamping head and the clamping groove. In this way, the carrying rod is fixed to the stud through the clamping head, and dismounting is convenient. The clamping head is provided with a guide pipe and a clamping protrusion, the stud is provided with a guide sleeve and a clamping opening, the guide pipe is sleeved with the guide sleeve, and the clamping protrusion is arranged in the clamping opening in a clamped mode. The utility model has the advantages that the transfer frequency of embryo samples in the vitrification process can be effectively reduced, so that embryo damage or embryo loss caused by transfer is avoided; the liquid loading area is arranged to replace a culture dish, so that the operation process of embryo vitrification is simplified, and the success efficiency of embryo vitrification is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of vitrification freezing technology for embryo samples, and more specifically, to an embryo vitrification freezing carrier rod. Background Art

[0002] The cryopreservation of embryo samples is a mature method for preserving fertility functions. This technology uses equilibration solution, cryoprotectant, etc. to pre-freeze the embryos, enabling the cryoprotectant to gradually penetrate into the cells of the embryos, so that the embryos can withstand the upcoming sharp temperature drop process without being damaged. After a series of operation procedures, the embryos can reach a suitable state for freezing, and then they can be placed in a liquid nitrogen environment at -196°C for long-term preservation.

[0003] Currently, in the field of assisted reproduction, during the embryo culture process, the traditional embryo vitrification freezing method is commonly used. The operation process is generally as follows: after preparing the equilibration solution and culture dish, prepare an equilibration solution drop in the culture dish; use a Pasteur pipette to transfer the embryo to the equilibration solution drop; let the embryo stand in the equilibration solution for 5 minutes; prepare a cryoprotectant solution drop in the culture dish; use a Pasteur pipette to transfer the embryo to the cryoprotectant solution drop; let the embryo stand in the cryoprotectant solution for 1 minute; use a Pasteur pipette to transfer the embryo to the front end of the freezing carrier rod; quickly immerse the front end of the freezing carrier rod into liquid nitrogen.

[0004] During the vitrification freezing process of embryos, it is necessary to transfer the embryo samples multiple times, which may cause a certain degree of damage to the embryos or embryo loss, and both a culture dish and a freezing carrier rod are required as consumables. Summary of the Utility Model

[0005] The technical problem to be solved by the utility model is to provide an embryo vitrification freezing carrier rod for the above-mentioned defects of the prior art, which simplifies the operation process of embryo vitrification freezing, effectively reduces the number of transfers of embryo samples during the vitrification freezing process, and improves the success rate of embryo vitrification freezing.

[0006] The technical solution adopted by the utility model to solve its technical problems is to propose an embryo vitrification freezing carrier rod, including a main body, on which a liquid loading area is provided, and a sample loading area is arranged in the liquid loading area.

[0007] Further, it further includes a stud, one end of the main body is provided with a clamping joint, the stud is provided with a clamping groove, the clamping joint is arranged in the clamping groove, and the main body is clamped on the stud through the cooperation of the clamping joint and the clamping groove. In this way, the carrier rod is fixed on the stud through the clamping joint, which is convenient for disassembly.

[0008] Further, a guiding tube and a clamping projection are provided on the clamping connector, a guiding sleeve and a clamping notch are provided on the stud, the guiding tube is sleeved in the guiding sleeve, and the clamping projection is clamped in the clamping notch.

[0009] Further, an external thread is provided on the outer periphery of the stud. In this way, the stud can be rotated onto the liquid nitrogen tank, and the vitrification freezing and sealing of the embryo can be conveniently achieved.

[0010] Furthermore, a screw port is provided at one end of the stud to facilitate tightening of the stud. The rotation of the stud can be conveniently controlled through the screw port.

[0011] Further, the liquid loading area is arranged at the other end of the body opposite to the clamping connector.

[0012] Further, the liquid loading area is a groove.

[0013] As an optimal option, the liquid loading area is a square groove. In this way,

[0014] Further, the sample loading area is arranged at the bottom surface of the groove in the liquid loading area.

[0015] As an optimal option, the sample loading area is a circular groove.

[0016] The advantages of the present utility model are as follows: it can effectively reduce the transfer times of embryo samples during vitrification freezing, thereby avoiding damage to the embryos or loss of embryos caused by transfer; by arranging a liquid loading area to replace the culture dish, the operation process of embryo vitrification freezing is simplified, and the success efficiency of embryo vitrification freezing is effectively improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present utility model will be further described below in conjunction with the drawings and embodiments. In the drawings:

[0018] Figure 1 is a schematic structural diagram of the present utility model;

[0019] Figure 2 is a schematic structural diagram of another perspective of the present utility model;

[0020] Figure 3 is a schematic structural diagram of the body;

[0021] Figure 4 is a schematic structural diagram of the stud;

[0022] Figure 5 is a schematic structural diagram of the liquid loading area and the sample loading area;

[0023] Figure 6 is Figure 5 the sectional view taken along line A-A in

[0024] In the figure: body 1 (wherein: card joint 1.1, guide tube 1.2, card projection 1.3), liquid loading area 2, sample loading area 3, stud 4 (wherein: card slot 4.1, guide sleeve 4.2, bayonet 4.3, thread 4.4, screw port 4.5). Detailed implementation mode

[0025] For a clearer understanding of the technical features, objectives, and effects of the present utility model, the detailed implementation mode of the present utility model will now be described in detail with reference to the accompanying drawings.

[0026] As Figures 1 to 2 , an embryo vitrification freezing carrier rod is constructed, including a body 1. A liquid loading area 2 is provided on the body 1, and a sample loading area 3 is provided in the liquid loading area 2. It further includes a stud 4. A card joint 1.1 is provided at one end of the body 1, a card slot 4.1 is provided on the stud 4, the card joint 1.1 is arranged in the card slot 4.1, and the body 1 is clamped on the stud 4 through the cooperation of the card joint 1.1 and the card slot 4.1. A guide tube 1.2 and a card projection 1.3 are provided on the card joint 1.1, a guide sleeve 4.2 and a bayonet 4.3 are provided on the stud 4, the guide tube 1.2 is sleeved in the guide sleeve 4.2, and the card projection 1.3 is clamped in the bayonet 4.3. A thread 4.4 is provided on the outer periphery of the stud 4. A screw port 4.5 is provided at one end of the stud 4 to facilitate tightening the stud 4. The liquid loading area 2 is arranged at the other end of the body 1 relative to the card joint 1.1. The liquid loading area 2 is a groove. The liquid loading area 2 is a square groove. The sample loading area 3 is arranged at the bottom of the groove of the liquid loading area 2. The sample loading area 3 is a circular groove.

[0027] When the patent of the present utility model is actually used:

[0028] A guide tube 1.2 is provided on the card joint 1.1 of the body 1. A plurality of card projections 1.3 are provided on the guide tube 1.2, and the plurality of card projections 1.3 are arranged at equal intervals. An indication groove 1.4 is also provided axially on the guide tube 1.2, and the indication groove 1.4 is used to indicate the direction in which the body 1 is clamped on the stud 4 in a specific direction. In one embodiment, three card projections 1.3 are provided on the guide tube 1.2, the indication groove 1.4 is arranged below the guide tube 1.2, the card projections 1.3 are arranged on the outer periphery of the guide tube 1.2, and the three card projections 1.3 are respectively arranged on both sides and the opposite side of the indication groove 1.4.

[0029] The liquid loading area 2 is used to drip and remove the equilibration solution and the cryoprotectant during the embryo vitrification freezing operation.

[0030] The sample loading area 3 is an embryo sample loading hole, which is used to place and position the embryo to prevent the embryo from moving driven by the flow of the liquid during the freezing process, and it is at the lowest position of the liquid loading area 2.

[0031] At the position on the stud 4 corresponding to the clamping protrusion 1.3, three clamping openings 4.3 are also provided. In this way, the insertion direction of the body 1 on the stud 4 can be ensured, and at the same time, the clamping force is enhanced to prevent the body 1 from slipping off the stud 4.

[0032] There is also a liquid nitrogen tank used in conjunction with the present utility model. The liquid nitrogen tank is provided with a screw port that cooperates with the thread 4.4. After the pre-freezing process of embryo vitrification freezing is completed, the bolt 4 is screwed into the screw port of the liquid nitrogen tank, so as to quickly freeze the embryo and improve the success rate of embryo vitrification freezing.

[0033] The specific operation process of the present utility model during embryo vitrification freezing is as follows:

[0034] 1. Insert the body 1 onto the bolt 4;

[0035] 2. Drop the prepared equilibration solution drop by drop into the liquid loading area 2, and pay attention to controlling that the equilibration solution drops do not drip into the sample loading area 3;

[0036] 3. Use a Pasteur pipette to transfer the embryo into the equilibration solution drop;

[0037] 4. Let the embryo stand still in the equilibration solution drop for 5 minutes;

[0038] 5. Drop the prepared cryoprotectant solution drop by drop into the liquid loading area 2 so that the cryoprotectant solution drop completely covers the sample loading area 3;

[0039] 6. Use a Pasteur pipette to transfer the embryo into the sample loading area 3 covered by the cryoprotectant solution drop;

[0040] 7. Let the embryo stand still in the sample loading area 3 for 1 minute, and suck out the equilibration solution drop in the liquid loading area 2;

[0041] 8. Use a Pasteur pipette to suck out the cryoprotectant solution drop in the liquid loading area 2;

[0042] 9. Screw the bolt 4 into the screw port of the liquid nitrogen tank. At this time, the sample loading area 3 of the body 1 quickly submerges the embryo into the liquid nitrogen, and finally close the liquid nitrogen tank.

[0043] The embodiments of the present utility model have been described above in conjunction with the accompanying drawings. However, the present utility model is not limited to the above specific embodiments. The above specific embodiments are merely illustrative and not restrictive. Under the inspiration of the present utility model, those of ordinary skill in the art can also make many forms without departing from the purpose of the present utility model and the scope protected by the claims. These all fall within the protection scope of the present utility model.

Claims

1. An embryo vitrification cryopreservation carrier rod, characterized in that, It includes a main body (1), a liquid loading area (2) is provided on the main body (1), and a sample loading area (3) is provided in the liquid loading area (2); It further includes a stud (4), a clamping joint (1.1) is provided at one end of the main body (1), a clamping groove (4.1) is provided on the stud (4), the clamping joint (1.1) is arranged in the clamping groove (4.1), and the main body (1) is clamped on the stud (4) through the cooperation of the clamping joint (1.1) and the clamping groove (4.1); A guide tube (1.2) and a clamping protrusion (1.3) are provided on the clamping joint (1.1), a guide sleeve (4.2) and a clamping notch (4.3) are provided on the stud (4), the guide tube (1.2) is sleeved in the guide sleeve (4.2), and the clamping protrusion (1.3) is clamped in the clamping notch (4.3).

2. The embryo vitrification cryopreservation carrier rod according to claim 1, characterized in that, External threads (4.4) are provided on the outer periphery of the stud (4).

3. The vitrification cryopreservation carrier rod for embryo according to claim 2, characterized in that, A screw thread (4.5) is provided at one end of the stud (4) to facilitate tightening of the stud (4).

4. The embryo vitrification cryopreservation carrier rod according to any one of claims 1 to 3, characterized in that, The liquid loading area (2) is arranged at the other end of the main body (1) relative to the clamping joint (1.1).

5. The embryo vitrification cryopreservation carrier rod according to claim 4, wherein, The liquid loading area (2) is a groove.

6. The embryo vitrification cryopreservation carrier rod according to claim 5, wherein The liquid loading area (2) is a square groove.

7. The embryo vitrification cryopreservation carrier rod according to claim 6, wherein The sample loading area (3) is arranged on the bottom surface of the groove of the liquid loading area (2).

8. The embryo vitrification cryopreservation carrier rod according to claim 7, wherein, The sample loading area (3) is a circular groove.

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