Tobacco black shank bacterium stress simulation experiment device

Abstract

The utility model belongs to the field of agricultural biology, and discloses a tobacco black shank pathogen stress simulation experiment device which comprises a supporting frame, an adjustable temperature and humidity control bin, an illumination adjusting system, a pathogen spraying system and an air circulation system. The adjustable temperature and humidity control bin is provided with multiple layers of temperature and humidity control bins, each layer is provided with a temperature and humidity sensor, and the environment in the bin can be monitored and adjusted in real time; an LED light source in the illumination adjusting system is mounted at the top of each layer of control bin and can be automatically adjusted; the automatic spraying system sprays a germ suspension through an adjustable nozzle; the air circulation system is installed on the side wall of the control bin, and air circulation is achieved through a fan and a filter screen. According to the utility model, the environmental condition that tobacco is stressed by phytophthora parasitica var nicotianae in the field can be simulated more accurately and efficiently, reliable experimental data is provided, and powerful technical support is provided for tobacco disease resistance research.

Description

Technical Field

[0001] The utility model belongs to, but is not limited to, the field of agricultural biotechnology, and particularly relates to an experimental device for simulating the stress of Phytophthora parasitica var. nicotianae Background Art

[0002] Under natural conditions, due to various reasons such as different geographical locations, climatic conditions and human activities, various adverse environments are created for plants, exceeding the range that plants can normally tolerate for growth and development, resulting in damage or even death of plants. These environments that cause damage to plants are called adversity or stress. Plants often suffer from various environmental stresses during growth, including abnormal temperatures, lack of water, and unfavorable soil chemical and physical conditions. However, the reduction of plant growth and crop yield caused by disease stress is becoming increasingly serious, which has attracted the widespread attention of scholars at home and abroad. Traditional stress simulation systems lack precise environmental control and are difficult to accurately simulate the pathogen infection environment.

[0003] Tobacco black shank is one of the most serious diseases in the world's tobacco production, especially in temperate, subtropical and tropical regions, and it is also one of the main diseases of tobacco in China. Among the culture equipment for pathogens to infect plants, most culture equipment is suitable for static environments and is difficult to simulate dynamic environmental conditions in the field, such as changes in temperature, humidity and light.

[0004] In view of the above analysis, the technical problems that urgently need to be solved in the prior art are as follows:

[0005] (1) Traditional stress simulation systems lack precise environmental control and are difficult to accurately simulate the pathogen infection environment;

[0006] (2) Most culture equipment is suitable for static environments and is difficult to simulate dynamic environmental conditions in the field, such as changes in temperature, humidity and light. Summary of the Invention

[0007] In view of the problems existing in the prior art, the utility model provides an experimental device for simulating the stress of Phytophthora parasitica var. nicotianae

[0008] The utility model is realized as follows. An experimental device for simulating the stress of Phytophthora parasitica var. nicotianae is characterized in that the experimental device for simulating the stress of Phytophthora parasitica var. nicotianae includes a support frame, an adjustable temperature and humidity control chamber, a light adjustment system, a pathogen spraying system and an air circulation system:

[0009] The support frame is located outside the entire device;

[0010] The adjustable temperature and humidity control chamber: The multi-layer temperature and humidity control chambers are arranged inside the frame. Each layer of the control chamber is fixed to the frame through a metal tray. Temperature and humidity sensors are installed on the inner walls of each layer of the control chamber. The sensors are connected to the central control unit through cables. The heating and humidifying devices are located at the bottom of the control chamber and are connected to each layer of the chamber body through pipes.

[0011] The light adjustment system: LED light sources are installed on the top of each layer of the control chamber, and the adjustment knobs are located on the side of the frame.

[0012] The germ spraying system: The automatic spraying system is installed on the inner top of the control chamber and is connected to the control chamber through a fixed bracket. The adjustable nozzle is connected to the spraying system and is connected to the germ storage tank through a hose. The position of the nozzle is adjustable, and the germ storage tank has a detachable design and is located on the side of the frame and is connected to the spraying system through a hose.

[0013] The air circulation system: The fans are installed on the side walls of the control chamber, fixed through brackets, and the rotation speed is adjustable. The filter screen is installed at the air inlet of the fan. The air circulation channels are built between each layer of the control chamber and are connected through ducts to ensure the circulation of air.

[0014] Furthermore, the support frame is made of stainless steel material, and the frame connects each component through bolts.

[0015] Furthermore, in the adjustable temperature and humidity control chamber, the multi-layer temperature and humidity control chambers, each chamber body is made of a transparent material (such as polycarbonate); for the heating and humidifying devices, the heating device heats the air inside the chamber through an electric heating element, and the humidifying device increases the air humidity through an evaporator.

[0016] Furthermore, in the light adjustment system, the LED light sources are fixed through brackets, and the position and angle can be adjusted manually; the adjustment knobs are connected to the LED light sources through cables and are used to adjust the light intensity.

[0017] Combined with the above technical solutions and the solved technical problems, the advantages and positive effects of the technical solution to be protected by the present utility model are as follows:

[0018] First, the present utility model can more accurately and efficiently simulate the environmental conditions of tobacco under the stress of black shank bacteria in the field, provide reliable experimental data, and provide strong technical support for the research on tobacco disease resistance.

[0019] Second, technical problems:

[0020] The present invention solves the problem of difficult precise control of temperature and humidity in the prior art. There are large deviations in temperature and humidity control in traditional stress simulation experimental devices, resulting in unstable experimental environments and poor repeatability and accuracy of results. Through a multi-layer temperature and humidity control chamber and a precise sensor system, the present invention achieves precise adjustment of temperature and humidity, ensuring uniform environmental conditions in each control chamber and greatly improving the reliability of experimental results.

[0021] In addition, the problem of non-adjustable light conditions in the prior art has also been effectively solved. The light systems of traditional devices usually cannot flexibly adjust the intensity and angle of the light source, and cannot simulate diverse environmental light conditions, affecting the diversity and precision of experimental results. The present invention realizes flexible adjustment of light intensity and angle through the adoption of an LED light source and an adjustable knob design, and can simulate light conditions in different environments, thereby meeting various experimental requirements.

[0022] The present invention also solves the problem of uneven distribution of pathogens. The existing pathogen spraying systems lack precise control, resulting in uneven distribution of pathogens on experimental samples and affecting the reliability of experimental results. Through an automatic spraying system and an adjustable nozzle design, the present invention ensures uniform spraying of pathogens on samples, making experimental results more stable and repeatable, and improving the scientificity and effectiveness of experiments.

[0023] Finally, the present invention improves the air circulation system and solves the problem of poor air circulation. The air circulation system design of traditional devices is imperfect, resulting in poor air circulation in the experimental environment and affecting temperature and humidity control and pathogen distribution. Through the design of efficient fans and air circulation channels, the present invention ensures good air circulation in each control chamber, maintains a stable temperature and humidity environment and uniform pathogen distribution, thereby significantly improving the overall performance of the experimental device and the reliability of experimental results. Description of the Drawings

[0024] Figure 1 is a schematic diagram of a tobacco black shank pathogen stress simulation experimental device provided by an embodiment of the present utility model;

[0025] Figure 2 is a schematic diagram of the internal structure of an adjustable temperature and humidity control chamber provided by an embodiment of the present utility model;

[0026] Figure 3 is a schematic diagram of the structure of a tobacco black shank pathogen stress simulation experimental device provided by an embodiment of the present utility model;

[0027] Figure 4 is a schematic diagram of a temperature and humidity control precision improvement curve provided by an embodiment of the present utility model;

[0028] Figure 5 is a schematic diagram of a pathogen distribution uniformity curve provided by an embodiment of the present utility model;

[0029] In the figure: 1. Support frame; 2. Adjustable temperature and humidity control chamber; 3. Metal tray; 4. Pathogen liquid storage tank; 5. Adjusting knob; 6. Fan; 7. LED light source; 8. Adjustable nozzle; 9. Heating device; 10. Humidifying device. Specific implementation manner

[0030] In order to make the objectives, technical solutions and advantages of the present utility model clearer, the present utility model will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0031] As Figure 1 shown, the tobacco black shank pathogen stress simulation experiment device provided by the embodiment of the present invention is characterized in that the tobacco black shank pathogen stress simulation experiment device includes a support frame 1, an adjustable temperature and humidity control chamber 2, a light intensity adjustment system, a pathogen spraying system and an air circulation system:

[0032] The support frame 1 is located outside the entire device;

[0033] As Figure 2 shown, in the adjustable temperature and humidity control chamber 2, multiple layers of temperature and humidity control chambers are arranged inside the frame. Each layer of control chamber is fixed to the frame through a metal tray 3. Temperature and humidity sensors are installed on the inner wall of each layer of control chamber. The sensors are connected to the central control unit through cables. The heating and humidifying device 10 is located at the bottom of the control chamber and is connected to each layer of the chamber body through pipelines;

[0034] In the light intensity adjustment system, the LED light source 7 is installed on the top of each layer of control chamber, and the adjusting knob 5 is located on the side of the frame;

[0035] In the pathogen spraying system, an automatic spraying system is installed on the inner top of the control chamber and is connected to the control chamber through a fixed bracket. The adjustable nozzle 8 is connected to the spraying system and is connected to the pathogen liquid storage tank 4 through a hose. The position of the nozzle is adjustable. The pathogen liquid storage tank 4 has a detachable design and is located on the side of the frame and is connected to the spraying system through a hose;

[0036] In the air circulation system, the fan 6 is installed on the side wall of the control chamber and is fixed through a bracket. The rotation speed is adjustable. A filter screen is installed at the air inlet of the fan 6. The air circulation channel is disposed between each layer of the control chamber and is connected through a conduit to ensure the circulation of air.

[0037] The support frame 1 is made of stainless steel material, and the frame connects each component through bolts.

[0038] The multi-layer temperature and humidity control bins in the adjustable temperature and humidity control bin 2 are each made of a transparent material (such as polycarbonate); a heating and humidifying device 10, where the heating device 9 heats the air in the bin through an electric heating element, and the humidifying device 10 increases the air humidity through an evaporator.

[0039] For the light intensity adjustment system, the LED light source 7 is fixed by a bracket, and its position and angle can be manually adjusted; the adjustment knob 5 is connected to the LED light source 7 through a cable for adjusting the light intensity.

[0040] Based on the tobacco black shank pathogen stress simulation experimental device, the following are two specific mechanical structure application examples:

[0041] Example 1: Precise control application of the pathogen spraying system

[0042] Application scenario:

[0043] In the tobacco black shank pathogen stress simulation experiment, in order to precisely simulate the pathogen infection process, quantitative pathogen spraying treatment needs to be carried out on tobacco plants.

[0044] Mechanical structure description:

[0045] Automatic spraying system: This system is installed at the inner top of the adjustable temperature and humidity control bin 2 and is tightly connected to the control bin through a fixed bracket to ensure the stability and uniformity of spraying.

[0046] Adjustable nozzle 8: The nozzle is connected to the spraying system and is connected to the pathogen liquid storage tank 4 through a hose. The position of the nozzle can be precisely adjusted according to experimental requirements to achieve precise spraying on different parts (such as leaves, stems, roots, etc.) of tobacco plants.

[0047] Pathogen liquid storage tank 4: It adopts a detachable design and is located on the side of the support frame 1, which is convenient for replacement and cleaning. It is connected to the spraying system through a hose to ensure the continuous supply of the pathogen suspension.

[0048] Implementation steps:

[0049] 1. According to the experimental design, adjust the position and angle of the nozzle to ensure that the pathogen suspension can be accurately sprayed onto the target parts of the tobacco plants.

[0050] 2. Turn on the pathogen spraying system and control the spraying amount through the adjustment knob 5 to achieve quantitative spraying treatment on tobacco plants.

[0051] 3. Observe and record the growth status and disease incidence of tobacco plants under pathogen stress.

[0052] Example 2: Circulation and filtration application of the air circulation system

[0053] Application scenario:

[0054] When simulating the infection environment of Phytophthora parasitica var. nicotianae, it is crucial to maintain the circulation and cleanliness of the air in the control chamber to avoid interference from other microorganisms.

[0055] Mechanical structure description:

[0056] Fan 6 and filter: Fan 6 is installed on the side wall of the control chamber and fixed by a bracket. Its rotation speed can be adjusted according to experimental requirements. The filter is installed at the air inlet of Fan 6 to effectively block external dust and microorganisms from entering the control chamber.

[0057] Air circulation channel: It is built between each layer of the control chamber and connected by ducts to form a complete air circulation system. Ensure that the air in the control chamber can be evenly distributed and maintain a certain flow rate.

[0058] Implementation steps:

[0059] 1. According to the experimental requirements, adjust the rotation speed of Fan 6 to control the air flow rate in the control chamber.

[0060] 2. Regularly check and clean the filter to ensure its good filtering effect and prevent external microorganisms from entering the control chamber and interfering with the experimental results.

[0061] 3. Observe and record the influence of the air circulation status in the control chamber on the growth of tobacco plants and the process of pathogen infection.

[0062] These two embodiments respectively show the specific applications of the pathogen spraying system and the air circulation system in the experimental device for simulating the stress of Phytophthora parasitica var. nicotianae, reflecting the important role of the mechanical structure in precisely controlling experimental conditions and maintaining the stability of the experimental environment.

[0063] As Figure 3 shown, the present utility model provides an experimental device for simulating the stress of Phytophthora parasitica var. nicotianae, which includes a support frame 1, an adjustable temperature and humidity control chamber 2, a light adjustment system, a pathogen spraying system, and an air circulation system.

[0064] The support frame 1 is made of stainless steel material and is located outside the entire device to ensure the stability and durability of the overall structure. The frame connects each component by bolts to ensure firmness.

[0065] The adjustable temperature and humidity control chamber 2 consists of multiple layers of temperature and humidity control chambers, temperature and humidity sensors, and heating and humidifying device 10. The multiple layers of temperature and humidity control chambers are arranged inside the frame. Each layer of the control chamber is fixed to the frame through a metal tray 3. Each chamber body is made of a transparent material (such as polycarbonate) to facilitate observing the internal situation. The temperature and humidity sensors are installed on the inner wall of each layer of the control chamber. The sensors are connected to the central control unit through cables to monitor and feedback environmental data in real time. The control unit adjusts the heating and humidifying device 10 according to the sensor data to maintain the required temperature and humidity conditions inside the chamber. The heating and humidifying device 10 is located at the bottom of the control chamber and is connected to each layer of the chamber body through pipes to control the temperature and humidity inside the chamber according to the data of the temperature and humidity sensors. The heating device 9 heats the air inside the chamber through an electric heating element, and the humidifying device 10 increases the air humidity through an evaporator.

[0066] For the light intensity adjustment system, the LED light source 7 is installed at the top of each layer of the control chamber. The light source is fixed by a bracket, and its position and angle can be manually adjusted. The adjustment knob 5 is located on the side of the frame and is connected to the LED light source 7 through a cable to adjust the light intensity. The position and angle of the light source can be manually adjusted to ensure that the light is evenly distributed on the surface of the tobacco plants inside the control chamber to simulate different light conditions.

[0067] For the pathogen spraying system, the automatic spraying system is installed at the inner top of the control chamber and is connected to the control chamber through a fixed bracket. It is started according to a preset program, and the pathogen suspension is transported from the liquid storage tank to the nozzle through a hose. The adjustable nozzle 8 is connected to the spraying system and is connected to the pathogen liquid storage tank 4 through a hose. The position of the nozzle can be adjusted to ensure uniform spraying. The nozzle sprays the pathogen suspension to ensure uniform coverage of the plant surface to simulate the pathogen stress environment. The pathogen liquid storage tank 4: The liquid storage tank has a detachable design and is located on the side of the frame for easy replacement and cleaning. It is connected to the spraying system through a hose.

[0068] For the air circulation system, the fan 6 is installed on the side wall of the control chamber and is fixed by a bracket. The rotation speed of the fan 6 can be adjusted. A filter screen is installed at the air inlet of the fan 6 to ensure filtering impurities when the air circulates. The air circulation channel is built between each layer of the control chamber and is connected through a conduit to ensure the circulation of air.

[0069] After the fan 6 is started, through the air circulation channel and the filter screen, it ensures the circulation of air inside the control chamber. The rotation speed of the fan 6 is adjusted to simulate the natural wind environment in the field, and the air flow intensity is controlled to ensure the stability and uniformity of air circulation.

[0070] Main components and models of the tobacco black shank pathogen stress simulation experimental device.

[0071] 1. Support frame:

[0072] Material: Stainless steel

[0073] Connection method: Bolt connection

[0074] 2. Adjustable temperature and humidity control chamber 2:

[0075] Number of layers: Multiple layers

[0076] Material: Transparent polycarbonate

[0077] Sensors: Temperature sensor (Model: DHT22), Humidity sensor (Model: HS1101)

[0078] Heating device 9: Electric heating element (Model: PTC heater)

[0079] Humidifying device 10: Ultrasonic humidifier (Model: UH-01)

[0080] Tray: Metal tray 3 (Model: MT-200)

[0081] Pipe connection: High-temperature silicone tube (Model: TS-100)

[0082] 3. Light adjustment system:

[0083] Light source: LED light source 7 (Model: Philips GreenPowerLED)

[0084] Fixed bracket: Adjustable bracket (Model: AS-300)

[0085] Adjusting knob 5: PWM dimmer (Model: PWM-05)

[0086] 4. Germ spraying system:

[0087] Spraying system: Automatic spraying system (Model: Nebulizer-500)

[0088] Nozzle: Adjustable nozzle 8 (Model: AN-02)

[0089] Liquid storage tank: Germ liquid storage tank 4 (Model: BR-10)

[0090] Hose: Medical-grade silicone tube (Model: SG-50)

[0091] 5. Air circulation system:

[0092] Fan 6: Variable-speed fan (Model: AF-200)

[0093] Bracket: Fixed bracket (Model: FS-100)

[0094] Filter net: HEPA filter net (Model: H13-200)

[0095] Air circulation channel: Built-in conduit (Model: AD-150)

[0096] Specific implementation:

[0097] The support frame 1 is made of stainless steel and connects each component by bolts to provide strong and stable support.

[0098] In the adjustable temperature and humidity control chamber 2, each layer is fixed to the frame through a metal tray 3. Temperature and humidity sensors (DHT22 and HS1101) are installed on the inner wall and connected to the central control unit through cables. The heating device 9 (PTC heater) and the humidifying device 10 (UH-01) are connected to each layer of the chamber through pipes.

[0099] In the light adjustment system, the LED light source 7 (Philips GreenPower LED) is fixed through a bracket (AS-300), and its position and angle can be adjusted manually. The adjustment knob 5 (PWM-05) is connected to the LED light source 7 through a cable to adjust the light intensity.

[0100] In the germ spray system, the automatic spray system (Nebulizer-500) is installed at the inner top of the control chamber and connected to the control chamber through a bracket. The adjustable nozzle 8 (AN-02) is connected to the spray system, and the liquid storage tank (BR-10) is connected to the spray system through a hose (SG-50).

[0101] In the air circulation system, the fan 6 (AF-200) is installed on the side wall of the control chamber and fixed through a bracket (FS-100). Its rotation speed is adjustable. The filter screen (H13-200) is installed at the air inlet of the fan 6. The air circulation channel is built-in between each layer of the control chamber and connected through a conduit (AD-150) to ensure the circulation of air.

[0102] The above are the main modules of the tobacco black shank pathogen stress simulation experiment device and their specific implementation devices and models.

[0103] The technical solution solves the problems of the prior art and the technical effects

[0104] Technical problems

[0105] 1. Uneven temperature and humidity control: Traditional stress simulation devices are difficult to achieve precise and uniform temperature and humidity control, resulting in poor repeatability and accuracy of experimental results.

[0106] 2. Unadjustable light conditions: Existing devices usually cannot flexibly adjust the light intensity and angle, and cannot simulate the light conditions in different environments, which has a great impact on the experiment.

[0107] 3. Uneven distribution of germs: The lack of precise control in the germ spraying system leads to uneven distribution of germs on the experimental samples, affecting the reliability of experimental results.

[0108] 4. Poor air circulation: The imperfect design of the air circulation system in the existing device results in poor air circulation in the experimental environment, affecting temperature and humidity control and germ distribution.

[0109] Technical effects

[0110] 1. Precise temperature and humidity control: Through the combination of multi-layer temperature and humidity control chambers, temperature and humidity sensors, and the heating and humidifying device 10, precise temperature and humidity control in each layer of the control chamber can be achieved, ensuring the stability and uniformity of the experimental environment.

[0111] 2. Adjustable lighting conditions: Through the design of the LED light source 7 and the adjustment knob 5, the lighting intensity and angle in each layer of the control chamber can be flexibly adjusted to simulate different lighting environments, improving the diversity and accuracy of the experiment.

[0112] 3. Uniform germ spraying: The combination of the automatic spraying system and the adjustable nozzle 8 can achieve uniform spraying of germs on the experimental samples, ensuring the reliability and repeatability of experimental results.

[0113] 4. Good air circulation: The design of the fan 6 and the air circulation channel ensures good air circulation in each layer of the control chamber, helping to maintain a stable temperature and humidity environment and uniform germ distribution.

[0114] To better demonstrate the above technical effects, the following two curve graphs are drawn:

[0115] 1. Curve of improved temperature and humidity control accuracy Figure 4

[0116] X-axis: Time (minutes)

[0117] Y-axis: Temperature and humidity deviation (°C / %RH)

[0118] Curve 1: Temperature and humidity deviation of the existing technology

[0119] Curve 2: Temperature and humidity deviation after improvement

[0120] 2. Curve of germ distribution uniformity Figure 5

[0121] X-axis: Sample position (number)

[0122] Y-axis: Germ concentration (CFU / cm 2 )

[0123] Curve 1: Germ distribution of the existing technology

[0124] Curve 2: Improved distribution of germs

[0125] The above are two curve graphs:

[0126] 1. Curve of improved temperature and humidity control accuracy:

[0127] The above figure shows the improvement in temperature control accuracy. The improved system significantly reduces the temperature deviation.

[0128] 2. Curve of uniformity of germ distribution:

[0129] The following figure shows the improvement in the uniformity of germ distribution. The improved system makes the distribution of germs on the sample more uniform.

[0130] These curves clearly show the significant improvement effects of the improved technical solution in terms of temperature and humidity control and germ distribution.

[0131] As described above, it is only the specific implementation manner of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention shall be covered by the protection scope of the present invention.

Claims

1. A simulation experimental device for Phytophthora parasitica var. nicotianae stress, characterized in that, It includes a support frame, an adjustable temperature and humidity control chamber, a light adjustment system, a germ spraying system, and an air circulation system: The support frame is located outside the entire device; For the adjustable temperature and humidity control chamber, multiple layers of temperature and humidity control chambers are arranged inside the frame. Each layer of the control chamber is fixed to the frame through a metal tray. Temperature and humidity sensors are installed on the inner wall of each layer of the control chamber. The sensors are connected to the central control unit through cables. The heating and humidifying devices are located at the bottom of the control chamber and are connected to each layer of the chamber body through pipes; For the light adjustment system, LED light sources are installed on the top of each layer of the control chamber, and the adjustment knobs are located on the side of the frame; For the germ spraying system, an automatic spraying system is installed on the inner top of the control chamber and is connected to the control chamber through a fixed bracket. An adjustable nozzle is connected to the spraying system and is connected to the germ liquid storage tank through a hose. The position of the nozzle is adjustable. The germ liquid storage tank has a detachable design and is located on the side of the frame and is connected to the spraying system through a hose; For the air circulation system, fans are installed on the side walls of the control chamber and are fixed through brackets. The rotation speed is adjustable. A filter screen is installed at the air inlet of the fan. The air circulation channels are placed between each layer of the control chamber and are connected through ducts to ensure the circulation of air.

2. The tobacco black shank pathogen stress simulation experimental device according to claim 1, characterized in that, The support frame is made of stainless steel material, and the frame connects each component through bolts.

3. The tobacco black shank pathogen stress simulation experimental device according to claim 1, characterized in that, For the multiple layers of temperature and humidity control chambers in the adjustable temperature and humidity control chamber, each chamber body is made of transparent material; for the heating and humidifying devices, the heating device heats the air in the chamber through an electric heating element, and the humidifying device increases the air humidity through an evaporator.

4. The tobacco black shank pathogen stress simulation experimental device according to claim 1, characterized in that, For the light adjustment system, the LED light sources are fixed through brackets, and the position and angle can be manually adjusted; the adjustment knobs are connected to the LED light sources through cables and are used to adjust the light intensity.

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