Full-automatic zebra fish micro-fluidic chip loading system

Abstract

The utility model belongs to the technical field of micro-fluidic chips, and particularly relates to a full-automatic zebra fish micro-fluidic chip loading system. Comprising a zebra fish container, a controllable micro-flow pipeline, a zebra fish micro-fluidic chip, a plurality of cameras and an upper computer, the zebra fish container is communicated with the zebra fish micro-fluidic chip through the controllable micro-flow pipeline, and the controllable micro-flow pipeline is further electrically connected with the upper computer; the plurality of cameras are respectively arranged in the controllable micro-flow pipeline and at the top of the zebra fish micro-fluidic chip, and the plurality of cameras are wirelessly connected with the upper computer; the utility model provides a full-automatic zebra fish micro-fluidic chip loading system in order to solve the problems that the existing zebra fish micro-fluidic chip loading process is tedious and time-consuming and the loading flux is generally low, and the full-automatic zebra fish micro-fluidic chip loading system is provided to solve the problems that the zebra fish micro-fluidic chip loading process is tedious and time-consuming in the prior art. The loading efficiency of the zebra fish micro-fluidic chip is improved, and the loading flux is improved on the basis.

Description

Technical Field

[0001] The utility model belongs to the technical field of microfluidic chips, and particularly relates to a fully automatic zebrafish microfluidic chip loading system. Background Art

[0002] Due to its high transparency, strong permeability, small size, easy operation and other characteristics, zebrafish has been widely used in many researches such as vertebrate life systems, complex diseases and drug screening. Among them, the manipulation and imaging of zebrafish are one of the important enabling technologies for realizing various researches.

[0003] The existing methods for manipulating zebrafish mainly include agarose, sodium methylcellulose fixation and anesthetic tricaine anesthesia. However, using agarose and methylcellulose for fixation is not only cumbersome in operation, but also prone to introducing artificial interference errors. At present, a zebrafish microfluidic chip has been developed to fix zebrafish larvae to avoid the use of agarose, sodium methylcellulose fixation and anesthetic tricaine. However, the process of loading zebrafish into the zebrafish microfluidic chip can only be achieved by manually controlling two syringes. This loading process is not only cumbersome and time-consuming, but also generally has the problem of low loading throughput. Summary of the Utility Model

[0004] In order to overcome the deficiencies of the prior art, the utility model provides a fully automatic zebrafish microfluidic chip loading system to solve the problem of the cumbersome and time-consuming loading process of the zebrafish microfluidic chip in the prior art, improve the loading efficiency of the zebrafish microfluidic chip, and on this basis, increase the loading throughput.

[0005] One solution of the utility model provides a fully automatic zebrafish microfluidic chip loading system, which includes a zebrafish container, a controllable microfluidic pipeline, a zebrafish microfluidic chip, multiple cameras and a host computer. The zebrafish container is communicated with the zebrafish microfluidic chip through the controllable microfluidic pipeline, and the controllable microfluidic pipeline is also electrically connected to the host computer; multiple cameras are respectively arranged in the controllable microfluidic pipeline and on the top of the zebrafish microfluidic chip, and all the multiple cameras are wirelessly connected to the host computer;

[0006] The zebrafish container contains multiple zebrafish, which are used to load the zebrafish microfluidic chip;

[0007] The controllable microfluidic pipeline is used to transport the zebrafish in the zebrafish container to the zebrafish microfluidic chip;

[0008] Multiple cameras are used to obtain the image information of the zebrafish in the controllable microfluidic pipeline and the zebrafish microfluidic chip;

[0009] The host computer is internally configured with a control main board, and both the controllable microfluidic tube and multiple cameras are connected to the host computer through the control main board;

[0010] The host computer is used to collect zebrafish image information transmitted by multiple cameras and send control commands to the controllable microfluidic pipeline.

[0011] It should be noted that the electrical connection between the controllable microfluidic pipeline and the host computer is achieved through the electrical connection between the switch on the controllable microfluidic pipeline and the control main board inside the host computer.

[0012] In this solution, the user can control the control main board inside the host computer through the host computer to automatically start loading zebrafish into the zebrafish microfluidic chip. During the loading process, the host computer automatically issues commands to the controllable microfluidic pipeline according to the zebrafish image information obtained from multiple cameras until the zebrafish microfluidic chip is loaded. This loading process eliminates the need for manual operation and improves the loading efficiency of the zebrafish microfluidic chip.

[0013] In one of the solutions, the controllable microfluidic pipeline includes an auxiliary pipeline, a fish delivery pipeline, a fish distribution pipeline that are connected in sequence, and multiple pipeline switches provided on the three of them. Multiple pipeline switches are all electrically connected to the host computer; a fish delivery camera for obtaining zebrafish image information in the fish delivery pipeline is also installed on the fish delivery pipeline;

[0014] The auxiliary pipeline is used to assist the fish delivery pipeline in sucking zebrafish into the fish delivery pipeline;

[0015] The fish delivery pipeline is used to transport zebrafish to the fish distribution pipeline;

[0016] The fish distribution pipeline is used to transport zebrafish into the zebrafish microfluidic chip.

[0017] In this solution, multiple pipeline switches are provided on the auxiliary pipeline, the fish delivery pipeline and the fish distribution pipeline, and a fish delivery camera is installed on the fish delivery pipeline, and all of them are wirelessly connected to the host computer, enabling the host computer to be able to obtain the zebrafish image information in the fish delivery pipeline in real time and correspondingly control the pipeline switches according to the image information to realize the control of the controllable microfluidic pipeline.

[0018] In one of the solutions, the output end of the auxiliary pipeline is communicated with one end of the zebrafish container, the input end of the auxiliary pipeline is connected with an auxiliary injection pump, the auxiliary injection pump is electrically connected to the host computer, and an auxiliary pipeline switch is also provided on the auxiliary pipeline.

[0019] In this solution, the host computer can control the working states of the auxiliary pipeline switch and the auxiliary injection pump, and cooperate with the zebrafish container connected to the auxiliary pipeline to make the zebrafish in the zebrafish container swim to the middle and upper layers of the liquid level of the zebrafish container, so as to suck the zebrafish into the fish delivery pipeline by the auxiliary fish delivery pipeline.

[0020] In one of the solutions, the input end of the fish delivery pipeline is connected to the zebrafish container, and the output end of the fish delivery pipeline is connected to the fish distribution pipeline;

[0021] The fish delivery pipeline includes a main microfluidic tube and a plurality of secondary microfluidic tubes. The input end of the main microfluidic tube is connected to the zebrafish container, the output end of the main microfluidic tube is connected to the fish distribution pipeline, a plurality of main pipeline switches are arranged on the main microfluidic tube, one ends of the plurality of secondary microfluidic tubes are all connected to the main microfluidic tube, fish delivery injection pumps are arranged at the other ends of the plurality of secondary microfluidic tubes, the plurality of fish delivery injection pumps are all electrically connected to the host computer, and secondary pipeline switches are arranged on the plurality of secondary microfluidic tubes.

[0022] In this solution, the host computer can suck the zebrafish in the zebrafish container into the fish delivery pipeline by controlling the working states of the main pipeline switch, the secondary pipeline switch and the plurality of fish delivery injection pumps, and at the same time cooperate with the fish delivery camera on the fish delivery pipeline to confirm the orientation of the zebrafish, and when the orientation needs to be adjusted, control the working states of the main pipeline switch, the secondary pipeline switch and the plurality of fish delivery injection pumps again to complete the orientation adjustment of the zebrafish and transport the zebrafish to the fish distribution pipeline.

[0023] In one of the solutions, a plurality of fixed cavities are arranged inside the zebrafish microfluidic chip, and a monitoring camera is arranged on the top of the zebrafish microfluidic chip.

[0024] In one of the solutions, the fish distribution pipeline includes branch microfluidic tubes corresponding to the number of the fixed cavities. The output ends of the branch microfluidic tubes are connected to the fixed cavities, and branch pipeline switches are arranged on the branch microfluidic tubes.

[0025] In this solution, the number of the fixed cavities can be changed according to the actual loading flux requirement. The number of the fixed cavities affects the number of the branch microfluidic tubes in the fish distribution pipeline, and the number of the branch microfluidic tubes affects the number of the branch pipeline switches. The host computer can control the working state of the branch pipeline switch to control the zebrafish to be transported into any fixed cavity.

[0026] In one of the solutions, the fixed cavity is a cavity structure that gradually changes from wide to narrow and then to wide along the water flow direction, and a narrow opening is arranged on the fixed cavity.

[0027] In one of the solutions, the cavity length of the fixed cavity is 3800 - 4500 μm, and the width is 500 - 950 μm.

[0028] In one solution, the length of the narrow opening of the fixed cavity is 380 - 450 μm, and the width is 250 - 350 μm.

[0029] In one solution, the host computer is configured with a control main board, and the control main board is used to send control commands to the controllable microfluidic pipeline. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0031] Figure 1 It shows a schematic structural diagram of the full-automatic zebrafish microfluidic chip loading system of the present invention;

[0032] Figure 2 It shows a working state diagram of the full-automatic zebrafish microfluidic chip loading system of the present invention;

[0033] Figure 3 It shows another working state diagram of the full-automatic zebrafish microfluidic chip loading system of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0035] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of the present invention, the directional indications are only used to explain the relative positional relationship and movement conditions between components in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0036] In addition, if the descriptions such as "first" and "second" are involved in the embodiments of the present utility model, the descriptions of "first", "second", etc. are only for descriptive purposes and should not be construed as indicating or implying their relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, if "and / or" or "and / or" appears throughout the text, its meaning includes three parallel scenarios. Taking "A and / or B" as an example, it includes Scenario A, or Scenario B, or the scenario where both A and B are satisfied simultaneously. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0037] Please refer to Figure 1 , in one of the embodiments, a fully automatic zebrafish microfluidic chip loading system is provided, which includes a zebrafish container, a controllable microfluidic pipeline, a zebrafish microfluidic chip, multiple cameras, and a host computer. The zebrafish container is communicated with the zebrafish microfluidic chip through the controllable microfluidic pipeline, and the controllable microfluidic tube is also electrically connected to the host computer; multiple cameras are respectively arranged in the controllable microfluidic pipeline and on the top of the zebrafish microfluidic chip, and multiple cameras are all wirelessly connected to the host computer;

[0038] The zebrafish container includes multiple zebrafish, and the zebrafish are used to load the zebrafish microfluidic chip;

[0039] The controllable microfluidic pipeline is used to transport the zebrafish in the zebrafish container to the zebrafish microfluidic chip;

[0040] Multiple cameras are used to obtain the image information of the zebrafish in the controllable microfluidic pipeline and the zebrafish microfluidic chip;

[0041] The host computer is internally configured with a control main board, and the controllable microfluidic tube and multiple cameras are both connected to the host computer through the control main board;

[0042] The host computer is used to collect the zebrafish image information transmitted by multiple cameras and send control commands to the controllable microfluidic pipeline.

[0043] It should be noted that the electrical connection between the controllable microfluidic pipeline and the host computer is realized by electrically connecting the switch on the controllable microfluidic pipeline to the control main board inside the host computer.

[0044] In this solution, the user can control the internal control main board through the host computer, send instructions to the controllable microfluidic pipeline, and make it automatically start loading zebrafish into the zebrafish microfluidic chip. During the loading process, the host computer automatically sends instructions to the controllable microfluidic pipeline according to the image information of zebrafish obtained from multiple cameras until the loading of the zebrafish microfluidic chip is completed. This loading process eliminates the need for manual operation and improves the loading efficiency of the zebrafish microfluidic chip.

[0045] In one of the solutions, the controllable microfluidic pipeline includes an auxiliary pipeline, a fish delivery pipeline, a fish distribution pipeline that are connected in sequence, and multiple pipeline switches provided on the three of them. The multiple pipeline switches are all electrically connected to the host computer; a fish delivery camera for obtaining the image information of zebrafish in the fish delivery pipeline is also installed on the fish delivery pipeline;

[0046] The auxiliary pipeline is used to assist the fish delivery pipeline in sucking zebrafish into the fish delivery pipeline;

[0047] The fish delivery pipeline is used to transport zebrafish to the fish distribution pipeline;

[0048] The fish distribution pipeline is used to transport zebrafish into the zebrafish microfluidic chip.

[0049] In this solution, by setting multiple pipeline switches on the auxiliary pipeline, the fish delivery pipeline and the fish distribution pipeline, installing a fish delivery camera on the fish delivery pipeline, and making them all electrically connected to the host computer, the host computer can obtain the image information of zebrafish in the fish delivery pipeline in real time, and correspondingly control the pipeline switches according to the image information to realize the control of the controllable microfluidic pipeline.

[0050] In one of the solutions, the output end of the auxiliary pipeline is connected to one end of the zebrafish container, the input end of the auxiliary pipeline is connected with an auxiliary injection pump, the auxiliary injection pump is electrically connected to the host computer, and an auxiliary pipeline switch is also provided on the auxiliary pipeline.

[0051] In this solution, the host computer can control the working states of the auxiliary pipeline switch and the auxiliary injection pump, and cooperate with the zebrafish container connected to the auxiliary pipeline to make the zebrafish in the zebrafish container swim to the middle and upper layers of the liquid level of the zebrafish container to assist the fish delivery pipeline in sucking the zebrafish.

[0052] In one of the solutions, the input end of the fish delivery pipeline is connected to the zebrafish container, and the output end of the fish delivery pipeline is connected to the fish distribution pipeline;

[0053] The fish delivery pipeline includes a main microfluidic tube and multiple secondary microfluidic tubes. The input end of the main microfluidic tube communicates with the zebrafish container, the output end of the main microfluidic tube communicates with the fish sorting pipeline, multiple main pipeline switches are provided on the main microfluidic tube, one ends of the multiple secondary microfluidic tubes are all communicated with the main microfluidic tube, fish delivery injection pumps are provided at the other ends of the multiple secondary microfluidic tubes, the multiple fish delivery injection pumps are all electrically connected to the host computer, and secondary pipeline switches are provided on the multiple secondary microfluidic tubes.

[0054] In this solution, the host computer can inhale the zebrafish in the zebrafish container into the fish delivery pipeline by controlling the working states of the main pipeline switch, the secondary pipeline switch, and the multiple fish delivery injection pumps, and at the same time cooperate with the fish delivery camera on the fish delivery pipeline to confirm the orientation of the zebrafish, and when the orientation needs to be adjusted, the working states of the main pipeline switch, the secondary pipeline switch, and the multiple fish delivery injection pumps are controlled again to complete the orientation adjustment of the zebrafish and transport the zebrafish to the fish sorting pipeline.

[0055] In one of the solutions, multiple fixed cavities are provided inside the zebrafish microfluidic chip, and a monitoring camera is provided on the top of the zebrafish microfluidic chip.

[0056] In one of the solutions, the fish sorting pipeline includes branch microfluidic tubes corresponding to the number of the fixed cavities. The output ends of the branch microfluidic tubes communicate with the fixed cavities, and branch pipeline switches are provided on the branch microfluidic tubes.

[0057] In this solution, the number of the fixed cavities can be changed according to the actual loading flux requirements. The number of the fixed cavities affects the number of the branch microfluidic tubes in the fish sorting pipeline, and the number of the branch microfluidic tubes affects the number of the branch pipeline switches. The host computer can control the working state of the branch pipeline switch to control the zebrafish to be transported into any fixed cavity.

[0058] In one of the solutions, the fixed cavity is a cavity structure that gradually changes from wide to narrow and then to wide along the water flow direction, and a narrow opening is provided on the fixed cavity.

[0059] In one of the solutions, the cavity length of the fixed cavity is 3800 - 4500 μm, and the width is 500 - 950 μm.

[0060] In one of the solutions, the narrow opening of the fixed cavity has a length of 380 - 450 μm and a width of 250 - 350 μm.

[0061] See Figures 1 - 3 , and the specific loading cases are as follows:

[0062] It should be noted that the fish delivery cameras C1 and C3 and the monitoring camera C2 are all wirelessly connected to the host computer CP through the local area network and realize image information transmission;

[0063] The auxiliary injection pump P1, the fish feeding injection pumps P2, P3, P4, the auxiliary pipeline switch S1, the main pipeline switch S2, the secondary pipeline switches S3, S4, the main pipeline switch S5, the secondary pipeline switch S6, the main pipeline switch S7, the branch pipeline switches S8, S9 and S10 are all electrically connected to the host computer CP through electrical connection with the control main board L;

[0064] The zebrafish microfluidic chip M is provided with a fixing cavity H1, a fixing cavity H2 and a fixing cavity H3, and a monitoring camera C2 is arranged on the top of the zebrafish microfluidic chip M;

[0065] An auxiliary pipeline switch S1 is arranged on the auxiliary pipeline. The output end of the auxiliary pipeline is communicated with the bottom of the zebrafish container R. The input end of the auxiliary pipeline is connected with an auxiliary injection pump P1, and the auxiliary injection pump P1 is electrically connected to the control main board L;

[0066] The zebrafish container R is communicated with the input end of the fish feeding pipeline;

[0067] Main pipeline switches S2, S5 and S7 are sequentially arranged on the main microtube of the fish feeding pipeline. The main microtube is communicated with the fish dividing pipeline; between the input end and the output end of the main microtube, one end of the first microtube, one end of the second microtube and one end of the third microtube are sequentially communicated. Secondary microtube switches S3, S4 and S6 are sequentially arranged on the corresponding secondary microtubes, and fish feeding injection pumps P2, P3 and P4 are sequentially arranged at the other ends of the corresponding secondary microtubes;

[0068] The input end of the fish dividing pipeline is communicated with the output end of the fish feeding pipeline. The input end of the fish dividing pipeline is three branch microtubes respectively communicated with the fixing cavity H1, the fixing cavity H2 and the fixing cavity H3.

[0069] Taking the loading of H1 as an example, the operator sets parameters through the host computer CP, specifies that the specific fixing cavity for loading zebrafish in the zebrafish microfluidic chip M is H1, and the host computer CP issues a control instruction to the controllable microfluidic pipeline through the control main board L:

[0070] It should be noted that in this case, the water flow direction is from the zebrafish container R, flowing through the fish feeding pipeline and the fish dividing pipeline in sequence, and finally flowing into the zebrafish microfluidic chip.

[0071] The control main board L controls the opening of the auxiliary pipeline switch S1, the main pipeline switch S2, and the secondary microfluidic pipeline switch S4, closes the main pipeline switch S5 and the secondary microfluidic pipeline switch S3, and then controls the auxiliary injection pump P1 to pump water or air from the E1 port of the zebrafish container R, so that the zebrafish in the zebrafish container R swim to the middle and upper layers of the liquid level of the zebrafish container R. At the same time, the fish delivery injection pump P3 is started to perform the air extraction work. Under the air extraction action of the fish delivery injection pump P3, a negative pressure is formed in the fish delivery pipeline, and the zebrafish in the zebrafish container R is inhaled to point A in the fish delivery pipeline through the E2 port of the zebrafish container R. At this time, the fish delivery camera C1 obtains the image information of the zebrafish and sends it to the host computer CP through the wireless local area network. The host computer CP receives the image information and processes it to obtain the orientation information of the zebrafish. During the period from when the host computer CP receives the image information to when it finishes processing to obtain the orientation information of the zebrafish, the host computer CP controls the auxiliary injection pump P1 and the fish delivery injection pump P3 to stop working, and the auxiliary pipeline switch S1 and the secondary microfluidic pipeline switch S4 are closed;

[0072] See Figure 2 , when the orientation information of the zebrafish is that the tail faces the water flow direction, the control main board L controls the closing of the auxiliary pipeline switch S1, the main pipeline switch S2, the secondary microfluidic pipeline switch S4, the secondary microfluidic pipeline switch S6, the branch pipeline switch S9, and the branch pipeline switch S10. The auxiliary injection pump P1, the fish delivery injection pump P3, and the fish delivery injection pump P4 do not work. The secondary microfluidic pipeline switch S3, the main pipeline switch S5, the main pipeline switch S7, and the branch pipeline switch S8 are opened, and the fish delivery injection pump P2 starts to pump water. The zebrafish at point A, under the action of the fish delivery injection pump P2, sequentially passes through the main pipeline switch S5, the main pipeline switch S7, and the branch pipeline switch S8 along the water flow, and is finally loaded into the fixing cavity H1. At this time, the tail of the zebrafish enters the fixing cavity H1, and the head of the zebrafish stays at the narrow opening of the fixing cavity H1;

[0073] See Figure 3, when the orientation information of the zebrafish is that the head of the fish faces the water flow direction, the control main board L controls the auxiliary pipeline switch S1, the main pipeline switch S2, the secondary microfluidic pipeline switch S3, the secondary microfluidic pipeline switch S4, the main pipeline switch S7, the branch pipeline switch S8, the branch pipeline switch S9 and the branch pipeline switch S10 to close, the auxiliary injection pump P1, the fish feeding injection pump P2 and the fish feeding injection pump P3 do not work, the main pipeline switch S5 and the secondary microfluidic pipeline switch S6 are opened, and the fish feeding injection pump P4 starts to perform air extraction work. Under the action of the fish feeding injection pump P4, the zebrafish at point A reaches point B along the water flow. At this time, the fish feeding camera C3 obtains the image information of the zebrafish and sends it to the host computer CP through the wireless local area network. The host computer CP receives the image information and processes it to obtain the orientation information of the zebrafish. During the period from when the host computer CP receives the image information to when it finishes processing to obtain the orientation information of the zebrafish, the host computer CP controls the fish feeding injection pump P4 to pause working, and the main pipeline switch S5 and the secondary microfluidic pipeline switch S6 are closed; when the orientation of the zebrafish is that the tail faces the water flow direction, the control main board L controls the branch pipeline switch S6, the main pipeline switch S7 and the branch pipeline switch S8 to open, the fish feeding injection pump P4 switches to the water pumping mode and resumes working. Under the action of the fish feeding injection pump P4, the zebrafish at point B passes through the branch pipeline switch S6, the main pipeline switch S7 and the branch pipeline switch S8 in sequence along the water flow and is finally loaded into the fixing cavity H1. At this time, the tail of the zebrafish enters the fixing cavity H1, and the head of the zebrafish stays at the narrow opening of the fixing cavity H1.

[0074] It should be noted that in one of the solutions, the operator can also, through the host computer CP, when setting parameters in the above case, specify that the fixing cavities H1, H2 and H3 in the zebrafish microfluidic chip M are all loaded with zebrafish. At this time, the control main board L will load the zebrafish in sequence according to the order of the fixing cavities H1, H2 and H3, and take the image information of the monitoring camera C2 as the standard. After the detection camera C2 can obtain the image information of the previous zebrafish, the above loading steps are cycled, and finally, through the branch pipeline switch S9 or the branch pipeline switch S10, they are correspondingly loaded into the fixing cavity H2 or the fixing cavity H3.

[0075] It should be noted that in one of the solutions, the loading flux can be improved by changing the number of fixing cavities in the zebrafish microfluidic chip M. Correspondingly, the operator only needs to replace the fish dividing pipeline with the corresponding number of fixing cavities in the zebrafish microfluidic chip M.

[0076] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. All equivalent structural transformations made under the inventive concept of the present invention by using the content of the specification and drawings of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A fully automatic zebrafish microfluidic chip loading system, characterized in that, It includes a zebrafish container, a controllable microfluidic pipeline, a zebrafish microfluidic chip, multiple cameras and a host computer. The zebrafish container is communicated with the zebrafish microfluidic chip through the controllable microfluidic pipeline, and the controllable microfluidic pipeline is also electrically connected to the host computer; multiple cameras are respectively arranged in the controllable microfluidic pipeline and on the top of the zebrafish microfluidic chip, and multiple cameras are wirelessly connected to the host computer; The zebrafish container contains multiple zebrafish, and the zebrafish are used to load the zebrafish microfluidic chip; The controllable microfluidic pipeline is used to transport the zebrafish in the zebrafish container to the zebrafish microfluidic chip; Multiple cameras are used to obtain image information of zebrafish in the controllable microfluidic pipeline and the zebrafish microfluidic chip; The host computer is internally configured with a control main board, and the controllable microfluidic pipeline and multiple cameras are both connected to the host computer through the control main board; The host computer is used to collect zebrafish image information transmitted by multiple cameras and send control commands to the controllable microfluidic pipeline.

2. The fully automatic zebrafish microfluidic chip loading system according to claim 1, wherein, The controllable microfluidic pipeline includes an auxiliary pipeline, a fish delivery pipeline, a fish distribution pipeline that are connected in sequence, and multiple pipeline switches arranged on the three of them. Multiple pipeline switches are all electrically connected to the host computer; a fish delivery camera for obtaining image information of zebrafish in the fish delivery pipeline is also installed on the fish delivery pipeline; The auxiliary pipeline is used to assist the fish delivery pipeline to suck zebrafish into the fish delivery pipeline; The fish delivery pipeline is used to transport zebrafish to the fish distribution pipeline; The fish distribution pipeline is used to transport zebrafish to the zebrafish microfluidic chip.

3. The fully automatic zebrafish microfluidic chip loading system according to claim 2, wherein The output end of the auxiliary pipeline is communicated with one end of the zebrafish container, the input end of the auxiliary pipeline is connected with an auxiliary injection pump, the auxiliary injection pump is electrically connected to the host computer, and an auxiliary pipeline switch is also arranged on the auxiliary pipeline.

4. The fully automatic zebrafish microfluidic chip loading system according to claim 2, wherein The input end of the fish delivery pipeline is communicated with the zebrafish container, and the output end of the fish delivery pipeline is communicated with the fish distribution pipeline; The fish delivery pipeline includes a main microfluidic tube and multiple secondary microfluidic tubes. The input end of the main microfluidic tube is communicated with the zebrafish container, the output end of the main microfluidic tube is communicated with the fish distribution pipeline, multiple main pipeline switches are arranged on the main microfluidic tube, one end of each of the multiple secondary microfluidic tubes is communicated with the main microfluidic tube, a fish delivery injection pump is arranged at the other end of each of the multiple secondary microfluidic tubes, multiple fish delivery injection pumps are all electrically connected to the host computer, and multiple secondary pipeline switches are arranged on the multiple secondary microfluidic tubes.

5. The fully automatic zebrafish microfluidic chip loading system according to claim 2, characterized in that, Multiple fixed cavities are arranged inside the zebrafish microfluidic chip, and a monitoring camera is arranged on the top of the zebrafish microfluidic chip.

6. The fully automatic zebrafish microfluidic chip loading system according to claim 5, wherein The fish distribution pipeline includes branch microfluidic tubes corresponding to the number of fixed cavities. The output end of the branch microfluidic tube is communicated with the fixed cavity, and branch pipeline switches are arranged on the branch microfluidic tubes.

7. The fully automatic zebrafish microfluidic chip loading system according to claim 5, characterized in that, The fixed cavity is a cavity structure that gradually changes from wide to narrow and then to wide along the water flow direction, and a narrow opening is arranged on the fixed cavity.

8. The fully automatic zebrafish microfluidic chip loading system according to claim 7, wherein The cavity length of the fixed cavity is 3800 - 4500μm, and the width is 500 - 950μm.

9. The fully automatic zebrafish microfluidic chip loading system according to claim 7, characterized in that, The length of the narrow opening of the fixed cavity is 380 - 450μm, and the width is 250 - 350μm.

10. The fully automatic zebrafish microfluidic chip loading system according to claim 1, wherein, The host computer is configured with a control main board, and the control main board is used to send control commands to the controllable microfluidic pipeline.

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