Light shielding device for elisa plate developing incubation

Abstract

The utility model discloses a light shielding device for developing incubation of an elisa plate. The light shielding device comprises a light shielding bottom plate and a light shielding cover which is adaptively buckled on the light shielding bottom plate, air holes are uniformly distributed in the light-proof bottom plate, upper ports and lower ports of the air holes are arranged in a staggered manner, and the middle part of the light-proof bottom plate is concave downwards to form a clamping groove for clamping and fixing an elisa plate; the light shielding cover is composed of a side surrounding plate and an upper sliding plate horizontally arranged on the top edge of the side surrounding plate in a sliding mode, the two ends of the upper sliding plate in the sliding direction horizontally extend outwards to form lengthened shielding sections, limiting check blocks are arranged on the bottom faces of the outer edges of the two lengthened shielding sections, an operation window is formed in the middle of the upper sliding plate, and a light shielding cover plate is hinged to the operation window. The kit has the advantages that the kit can be used for shading and covering the elisa plate in all directions, shading conditions for developing and incubating the elisa plate can be easily realized, good air permeability can be ensured, light rays can be effectively prevented from entering, the temperature of the inner side of the shading cover can be easily and accurately controlled, the inner side of the shading cover can quickly reach a constant-temperature condition, and the working efficiency of the elisa plate is improved. The sensitivity and the accuracy of color development incubation are ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of light-shielding incubation for enzyme-linked immunosorbent assay, in particular to a light-shielding device for color development incubation of an enzyme-labeled plate. Background Technique

[0002] In enzyme-linked immunosorbent assay, after the enzyme-labeled substance binds to a specific antigen or antibody, color development incubation needs to be carried out at an appropriate temperature to enable the substrate to react with the analyte to produce a product with a specific color for subsequent analysis and detection; among them, the sensitivity and accuracy of the color development reaction are crucial for the experimental results, and the key in the color development reaction process lies in light shielding, that is, it needs to be kept in a light-shielded dark environment for a long time.

[0003] At present, the existing light-shielding boxes, light-shielding covers, etc. have poor light-shielding effects on enzyme-labeled plates and cannot ensure full-range light shielding. In particular, they cannot provide good light-shielding for the transparent bottom of the enzyme-labeled plate. Moreover, the light-shielding boxes and light-shielding covers are relatively closed and have poor air permeability, resulting in poor control of the constant temperature inside after covering the light-shielding box or light-shielding cover, affecting the accuracy of the color development reaction results; in addition, when the existing light-shielding boxes and light-shielding covers are opened for observation or sample addition, all the wells on the enzyme-labeled plate are fully exposed, resulting in the entire enzyme-labeled plate being easily contaminated or showing incomplete color development and other phenomena, thereby interfering with the final result of the color development reaction and greatly reducing the accuracy. Summary of the Invention

[0004] The purpose of the utility model is to provide a light-shielding device for color development incubation of an enzyme-labeled plate in view of the defects of the prior art.

[0005] To achieve the above purpose, the utility model can adopt the following technical solutions:

[0006] The light-shielding device for color development incubation of an enzyme-labeled plate described in the utility model includes a light-shielding bottom plate and a light-shielding cover that is adaptively buckled thereon; wherein, ventilation holes are evenly distributed on the light-shielding bottom plate, and the upper and lower ports of the ventilation holes are arranged in a staggered manner. A card slot for clamping the enzyme-labeled plate is formed by the middle part of the light-shielding bottom plate being recessed downward; the light-shielding cover is composed of a side surrounding plate and an upper sliding plate that is horizontally slidably arranged on the top edge of the side surrounding plate. The two ends of the upper sliding plate in the sliding direction horizontally extend outward to form extended shielding sections. Limit blocks are arranged on the bottom surfaces of the outer edges of the two extended shielding sections. An operation window is opened in the middle of the upper sliding plate, and a light-shielding cover plate is hinged on the operation window.

[0007] Specifically, a pair of horizontal chutes with opposite notch directions are arranged in parallel on the top edge of the side panel, and the upper slide plate is slidably arranged in the two horizontal chutes; an annular slot for fitting and inserting the side panel is arranged on the top surface of the light-shielding bottom plate; in addition, in order to facilitate the overall handling, moving of the light-shielding bottom plate and the light-shielding cover, a holding handle can also be arranged on the opposite side edges of the light-shielding bottom plate; in order to enable air to easily enter the light-shielding cover through the air holes, feet can also be arranged at the four corners of the bottom surface of the light-shielding bottom plate, so as to have a certain space below the light-shielding bottom plate to facilitate air circulation.

[0008] The advantages of the present utility model are that the enzyme-labeled plate can be covered from light in all directions through the light-shielding bottom plate and the light-shielding cover buckled thereon, and the light-shielding condition for the color development incubation of the enzyme-labeled plate can be easily realized, ensuring that the enzyme-labeled plate can be kept in a light-shielded dark environment for a long time; at the same time, the upper and lower ports of the air holes uniformly distributed on the light-shielding bottom plate are arranged in a staggered manner, which can also ensure good air permeability and effectively block light from entering, easily and accurately control the temperature inside the light-shielding cover, quickly reach the constant temperature condition inside the light-shielding cover, and ensure the sensitivity and accuracy of the color development incubation; in addition, the operation window with a light-shielding cover plate opened in the middle of the upper slide plate can also facilitate observation or sample addition, and reduce the exposure of the enzyme-labeled plate as much as possible during the observation or sample addition process, avoid the contamination or incomplete color development of the enzyme-labeled plate, avoid interfering with the final result of the color reaction, and ensure the accuracy of the color reaction of the enzyme-labeled plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 is a schematic structural diagram of the present utility model.

[0010] Figure 2 is Figure 1 The enlarged sectional view taken along the line A-A of the light-shielding bottom plate in (hiding the holding handle). DETAILED DESCRIPTION OF THE EMBODIMENTS

[0011] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

[0012] As Figure 1 shown, the light-shielding device for the color development incubation of the enzyme-labeled plate described in the present utility model includes a light-shielding bottom plate 1 and a light-shielding cover 2 adapted to be buckled thereon.

[0013] Specifically, a plurality of air holes 3 are uniformly distributed on the light-shielding bottom plate 1, and the upper port and the lower port of each air hole 3 are arranged in a staggered manner, thereby forming a pore channel with an approximate Z-shaped structure (as Figure 2As shown), it can effectively block light from passing through while ensuring good ventilation; in addition, a card slot 4 is formed in the middle of the upper surface of the light-shielding bottom plate 1, and the card slot 4 is a rectangular structure that can accommodate the enzyme labeling plate.

[0014] The light-shielding cover 2 is composed of a side panel 2.1 and an upper slide plate 2.2 which is horizontally slidably arranged on the top edge of the side panel 2.1. In order to realize the sliding connection between the upper slide plate 2.2 and the side panel 2.1, a pair of horizontal slide grooves 5 with facing notches can be arranged in parallel on the top edge of the side panel 2.1. The edge of the upper slide plate 2.2 matches the two horizontal slide grooves 5 and is slidably arranged in the two horizontal slide grooves 5.

[0015] In addition, in order to always cover the top opening of the side panel 2.1, an extended shielding section 6 is extended horizontally outward at both ends of the sliding direction of the upper slide 2.2, and a limit block 7 for blocking the outside of the side panel 2.1 is provided on the outer edge bottom surface of the two extended shielding sections 2.3, so that when the upper slide 2.2 slides horizontally on the top of the side panel 2.1, it can completely cover the top opening of the side panel 2.1 regardless of sliding to the left or right; at this time, an operation window 8 is opened in the middle position of the upper slide 2.2, and a light-proof cover 9 is hinged on the operation window 8. The position of the operation window 8 can be adjusted by sliding the upper slide 2.2 to the left or right, so that the ELISA plate located in the side panel 2.1 can be observed or sampled in all directions using the operation window 8.

[0016] Furthermore, to achieve the interlocking fit between the light-shielding base plate 1 and the light-shielding cover 2, an annular slot 10 can be provided on the top surface of the light-shielding base plate 1, adapted to fit the bottom edge of the side panel 2.1. In this case, the ventilation holes 3 are located on the light-shielding base plate 1 inside the annular slot 10. To facilitate the overall transport and movement of the light-shielding base plate 1 and light-shielding cover 2, gripping handles 11 can be provided on opposite sides of the light-shielding base plate 1. To allow air to easily enter the light-shielding cover 2 through the ventilation holes 3, supporting legs 12 can be provided at the four corners of the bottom surface of the light-shielding base plate 1, thereby creating a certain space below the light-shielding base plate 1 for air circulation.

[0017] When in use, just need to firmly place the ELISA plate that needs to be color-incubated in the slot 4 in the middle of the upper surface of the light-proof base plate 1, and then buckle the light-proof cover 2 to place it in the constant temperature incubation box for color-incubation. The light-proof base plate 1 and the light-proof cover 2 can cover the ELISA plate from light in all directions, easily achieve the light-proof condition for color-incubation of the ELISA plate, and ensure that the ELISA plate can be kept in a dark environment away from light for a long time. The upper and lower ports of the air holes 3 evenly distributed on the light-proof base plate 1 are staggered, and can effectively block the entry of light while ensuring good air permeability, so that the inside of the light-proof cover 2 can quickly reach the constant temperature condition under the action of the good air permeability of the light-proof base plate 1, thereby realizing precise control of the temperature inside the light-proof cover 2 and ensuring the sensitivity and accuracy of color-incubation.

[0018] When it is necessary to observe or add samples to the enzyme-labeled plate for color development incubation, the upper slide plate 2.2 can be slid left or right, so as to adjust the position of the operation window 8, and the light-shielding cover plate 9 can be opened for observation, or the pipette gun can be inserted into the operation window 8 for adding samples. During the observation or sample addition process, the exposure of the enzyme-labeled plate can be minimized as much as possible, avoiding contamination or incomplete color development of the enzyme-labeled plate, avoiding interference with the final result of the color development reaction, and ensuring the accuracy of the color development reaction of the enzyme-labeled plate.

[0019] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relative positional relationship and movement conditions between components in a certain specific posture (as shown in the drawings). If the specific posture changes, the directional indications will also change accordingly.

Claims

1. A light-shielding device for color development and incubation of enzyme-linked immunosorbent assay (ELISA) plates, characterized in that: It includes a light-proof base plate and a light-proof cover that fits thereon; wherein, the light-proof base plate is evenly distributed with air holes, the upper port and the lower port of the air holes are staggered, and the middle part of the light-proof base plate is concave to form a slot for fixing the ELISA plate; the light-proof cover is composed of side panels and an upper slide plate that is horizontally slidably arranged on the top edge of the side panels, and the two ends of the sliding direction of the upper slide plate extend horizontally outward to form an extended shielding section, and limited stop blocks are arranged on the outer bottom surface of the two extended shielding sections, and an operation window is opened in the middle of the upper slide plate, and a light-proof cover plate is hinged on the operation window.

2. The light-shielding device for color development incubation of an enzyme-labeled plate according to claim 1, wherein: A pair of horizontal slides with notches facing each other are arranged in parallel on the top edge of the side panel, and the upper slide plate is slidably arranged in the two horizontal slides.

3. The light-shielding device for color development incubation of an enzyme-labeled plate according to claim 1, wherein: An annular slot adapted to be plugged into the side panels is provided on the top surface of the light-proof bottom plate.

4. The light-shielding device for color development and incubation of enzyme-labeled plates according to claim 1, characterized in that: Gripping handles are provided on opposite side edges of the light-shielding bottom plate.

5. The light-shielding device for color development incubation of an enzyme-labeled plate according to claim 1, wherein: Support legs are arranged at the four corners of the bottom surface of the light-shielding bottom plate.

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