Plant volatile gas continuous collection device

Abstract

The utility model relates to a plant volatile gas continuous collection device, which comprises a culture device, a gas inlet filtering device and a gas collection device, the top of the culture device is provided with a gas inlet, the gas inlet is connected with the gas inlet filtering device through a gas inlet pipeline, and the side wall of the middle part of the culture device is provided with a gas outlet; a butyl rubber plug is arranged at the air outlet, the gas collecting device comprises a vacuum pump and an adsorption needle head containing an adsorbent, a needle tube of the adsorption needle head penetrates through the butyl rubber plug, a needle base of the adsorption needle head is connected with a Luer lock, and the Luer lock is connected with the vacuum pump through an exhaust pipe. The device is simple in structure, scientific in design, easy to disassemble and assemble and low in manufacturing cost, and is made of a durable material with high cost performance, so that the cost is effectively controlled, the device is long in service life and can be repeatedly used, and the green and environment-friendly concept is manifested; the culture device creates a stable growth air environment for the plants, and continuous and in-situ collection of volatile gas of the plants is achieved through the connection of the adsorption needles.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a continuous collection device for plant volatile gases. Background Art

[0002] Plant volatile gases refer to a series of low-molecular-weight, high-vapor-pressure, and easily volatile organic compounds released by plants into the atmosphere during their growth process. The volatile gases of plants show potential and application prospects in the prevention and control of agricultural diseases. The volatile gases of plants can serve as signal molecules for plants to communicate and interact with the surrounding environment, and have multiple functions in the ecosystem. They not only affect the physiology and ecology of plants themselves, but also play a key role in aspects such as agricultural disease prevention and control, ecosystem balance, and atmospheric chemical processes. Due to the rapid diffusion, low concentration, and unstable release rate of the volatile gas molecules emitted by plants, it is not easy to collect and enrich them, and it is even more difficult to achieve real-time collection. At present, the reported extraction methods mainly include liquid nitrogen condensation method, static collection method, gas flow type liquid phase microextraction device collection method, adsorption extraction, etc. These methods all require some special equipment, which is usually expensive, complex to operate, difficult to achieve real-time collection, and requires professional maintenance, increasing the collection cost. Content of the Utility Model

[0003] In view of this, the purpose of the utility model is to provide a continuous collection device for plant volatile gases with a simple structure, easy disassembly and assembly, and low manufacturing cost.

[0004] The utility model adopts the following scheme: A continuous collection device for plant volatile gases, including a cultivation device, an air inlet filtering device, and a gas collection device. An air inlet is provided at the top of the cultivation device, and the air inlet is connected to the air inlet filtering device through an air inlet pipeline. An air outlet is provided on the middle side wall of the cultivation device; a butyl rubber stopper is provided at the air outlet. The gas collection device includes a vacuum pump and an adsorption needle head containing an adsorbent inside. The needle tube of the adsorption needle head passes through the butyl rubber stopper, and a Luer lock is connected to the needle seat of the adsorption needle head. The Luer lock is connected to the vacuum pump through an air extraction pipe.

[0005] Further, the cultivation device includes a base, an isolation cover, and a top cover. A sealing rubber ring is provided between the top cover and the isolation cover. The cultivation device is cylindrical, and the base, the isolation cover, and the top cover are all made of transparent acrylic material.

[0006] Further, an air inlet valve is provided at the air inlet, and the top cover is connected to the upper end of the isolation cover through a butterfly wing bolt.

[0007] Further, the air intake filtering device includes a conical flask filled with activated carbon inside. A stopper is provided at the mouth of the conical flask, and two through holes are opened in the stopper. Glass tubes are inserted through both of the two through holes. The lower end of one glass tube is inserted into the activated carbon, and the upper end of the other glass tube is connected to the air inlet pipeline.

[0008] Further, rotameters are connected in series on both the air inlet pipeline and the air extraction pipeline; a glass filter column is also connected in series on the air inlet pipeline, and two masses of absorbent cotton are provided at both ends of the glass filter column.

[0009] Compared with the prior art, the present utility model has the following beneficial effects:

[0010] (1) The structure is concise, scientifically designed, easy to disassemble and assemble, with low manufacturing cost. High-cost performance and durable materials are adopted, effectively controlling costs while ensuring a long service life of the device and being reusable, demonstrating the concept of green environmental protection;

[0011] (2) The cultivation device creates a stable growth air environment for plants, and the access of the adsorption needle realizes the continuous and in-situ collection of plant volatile gases; it can continuously collect and dynamically monitor plant volatile gases without disturbing the natural growth state of plants, ensuring the accuracy and continuity of data;

[0012] (3) It has excellent isolation performance, ensuring the controllable cultivation of plants in an environment free from external microbial intrusion, and is suitable for exploring the volatile gas response mechanisms of plants under biotic stress (such as pest and disease infestation) and abiotic stress (such as nutrient stress) conditions.

[0013] In order to make the purpose, technical solution and advantages of the present utility model clearer, the following will further elaborate on the present utility model through specific embodiments and related drawings. Description of the Drawings

[0014] Figure 1 is a schematic structural diagram of an embodiment of the present utility model;

[0015] Figure 2 is a schematic diagram of the connection between the adsorption needle and the Luer lock in an embodiment of the present utility model;

[0016] Explanation of the reference numerals in the drawings: 1 - activated carbon; 2 - absorbent cotton; 3 - rotameter; 4 - air inlet pipeline; 5 - isolation cover; 6 - air inlet valve; 7 - rubber sealing ring; 8 - butterfly yoke bolt; 9 - adsorption needle; 10 - butyl rubber stopper; 11 - Luer lock; 12 - vacuum pump; 13 - air intake filtering device; 14 - top cover. Detailed Embodiments

[0017] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.

[0018] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0019] As Figures 1 - 2 shown, a continuous collection device for plant volatile gases includes a cultivation device, an intake air filtration device 13, and a gas collection device. The cultivation device is provided with an intake port at the top, and the intake port is connected to the intake air filtration device 13 through an intake pipeline 4. An air outlet is provided on the side wall in the middle of the cultivation device; a butyl rubber stopper 10 is provided at the air outlet. The gas collection device includes a vacuum pump 12 and an adsorption needle 9 containing an adsorbent inside. The needle tube of the adsorption needle passes through the butyl rubber stopper 10, and the needle seat of the adsorption needle 9 is connected with a Luer lock 11. The Luer lock 11 is connected to the vacuum pump 12 through an extraction tube. The vacuum pump is the guarantee for the natural growth of plants and is the key component for collecting plant volatile gases; the adsorbent adopts a volatile organic compound adsorbent (Tenax TA + Carboxene1016 + Carboxen 1000). When pumping air with the vacuum pump, the gas in the cultivation device is sucked in through the intake air filtration device and discharged through the air outlet at the butyl rubber stopper; fresh air continuously enters the cultivation device through the intake air filtration device, and the gas discharged from the cultivation device finally enters the collection device, thus completing the collection and enrichment of volatile gases. The collection device of the present utility model solves the problem of continuously collecting plant volatile gases without disturbing the normal growth conditions of plants; solves the problem of external stress suffered by plants due to leaving the original growth environment, thereby interfering with the components of plant volatile gases; solves the difficulty of not being easy to collect and enrich due to too fast volatilization in the collection of plant volatile gases.

[0020] In this embodiment, the cultivation device includes a base, an isolation cover 5, and a top cover 14. A sealing rubber ring 7 is provided between the top cover 14 and the isolation cover 5. The cultivation device is cylindrical, and the base, the isolation cover, and the top cover are all made of transparent acrylic material; the isolation cover and the base are fixedly connected together and can be fixedly glued with glass glue.

[0021] In this embodiment, both the intake pipeline 4 and the extraction tube are made of silica gel tubes, and the Luer lock 11 is connected to the extraction tube through an adapter.

[0022] In this embodiment, an intake valve 6 is provided at the air inlet, and the top cover 14 is connected to the upper end of the isolation cover 5 by butterfly eye bolts 8.

[0023] In this embodiment, the intake air filtering device 13 includes a conical flask internally filled with activated carbon 1. A stopper is provided at the mouth of the conical flask, and two through holes are opened in the stopper. Glass tubes are inserted through both of the two through holes. The lower end of one of the glass tubes is inserted into the activated carbon, and the upper end communicates with the outside air, ensuring that the air entering the culture device from the outside can be adsorbed by the activated carbon. The upper end of the other glass tube is connected to the intake pipeline, and the lower end is higher than the upper surface height of the activated carbon by about 2 cm, so as to ensure that the air adsorbed by the activated carbon can enter the adsorption column through this pipeline.

[0024] In this embodiment, rotameters 3 are connected in series on both the intake pipeline 4 and the extraction pipeline. The rotameters 3 are used for device leak detection. When the flow rates at both ends are the same, it indicates that the device has good airtightness, ensuring that the gas flow rates in and out of the device are the same, and keeping the air environment inside the device in a stable state. A glass filter column is also connected in series on the intake pipeline, and two masses of absorbent cotton 2 are provided at both ends of the glass filter column; to prevent solid impurities in the gas from entering the culture device and affecting the results.

[0025] During use, first place the plants for which gas needs to be collected in the isolation cover, install the top cover, and then connect the gas collection device and the intake air filtering device. After the plants grow normally, the collection of plant volatile gases can be carried out. After culturing for a period of time, the volatile gases are collected and enriched through the gas collection device. The culture device is isolated from the outside world, which can ensure that the collected plant volatile gases are relatively in an ideal state without the pollution of other substances. This device is easy to assemble, is conducive to carrying, and has a relatively low cost.

[0026] For any of the technical solutions disclosed by the present utility model as described above, unless otherwise stated, if it discloses a numerical range, the disclosed numerical range is a preferred numerical range. Any person skilled in the art should understand that the preferred numerical range is only the numerical values with relatively obvious technical effects or representativeness among many implementable numerical values. Since there are too many numerical values to list them all, the present utility model only discloses some numerical values to illustrate the technical solutions of the present utility model. Moreover, the numerical values listed above should not constitute a limitation on the protection scope of the present utility model creation.

[0027] If the present utility model discloses or involves components or structural parts that are fixedly connected to each other, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (for example, using bolts or screws for connection), or can also be understood as: a non-detachable fixed connection (for example, riveting, welding). Of course, the mutual fixed connection can also be replaced by an integral structure (for example, manufactured by integral forming using a casting process) (except when it is obviously impossible to adopt the integral forming process).

[0028] In addition, for the terms used to represent the positional relationship or shape in any of the technical solutions disclosed in the present utility model above, unless otherwise stated, their meanings include states or shapes that are approximate, similar or close thereto.

[0029] Any component provided by the present utility model can either be assembled from a plurality of separate components or be a single component manufactured by an integral forming process.

[0030] The above are only the preferred embodiments of the present utility model, and are not intended to limit the present utility model in other forms. Any person skilled in the art may use the technical content disclosed above to make changes or modifications into equivalent embodiments with equivalent changes. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the content of the technical solution of the present utility model still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A continuous collection device for plant volatile gases, characterized in that: It includes a culture device, an air inlet filtering device and a gas collection device. An air inlet is provided at the top of the culture device. The air inlet is connected to the air inlet filtering device through an air inlet pipeline. An air outlet is provided on the side wall in the middle of the culture device; a butyl rubber stopper is provided at the air outlet. The gas collection device includes a vacuum pump and an adsorption needle head containing an adsorbent inside. The needle tube of the adsorption needle head passes through the butyl rubber stopper. The needle seat of the adsorption needle head is connected with a Luer lock. The Luer lock is connected to the vacuum pump through an air extraction pipe.

2. The continuous collection device for plant volatile gases according to claim 1, wherein: The culture device includes a base, an isolation cover and a top cover. A sealing rubber ring is provided between the top cover and the isolation cover. The culture device is cylindrical. The base, the isolation cover and the top cover are all made of transparent acrylic material.

3. The continuous collection device for plant volatile gases according to claim 2, characterized in that: An air inlet valve is provided at the air inlet. The top cover is connected to the upper end of the isolation cover through a butterfly toggle bolt.

4. The continuous collection device for plant volatile gases according to claim 1, characterized in that: The air inlet filtering device includes a conical flask filled with activated carbon inside. A bottle stopper is provided at the bottle mouth of the conical flask. Two through holes are provided on the bottle stopper. Glass tubes are inserted through both of the two through holes. The lower end of one glass tube is inserted into the activated carbon, and the upper end of the other glass tube is connected to the air inlet pipeline.

5. The continuous collection device for plant volatile gases according to claim 1, characterized in that: Rotameters are connected in series on both the air inlet pipeline and the air extraction pipe; a glass filter column is also connected in series on the air inlet pipeline. Two balls of absorbent cotton are provided at both ends of the glass filter column.

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