Cell culture dish suitable for liquid-changing culture of biological cells
By introducing the liquid inlet interface, liquid inlet valve, liquid outlet interface and flow guide into the cell culture dish, the cell liquid replacement operation is realized in a closed environment, solving the problems of inconvenient operation and high contamination risks in the prior art, and improving the safety and convenience of the liquid replacement process.
Patent Information
- Application Number
- CN202422059090.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-23
- Publication Date
- 2025-08-05
- Estimated Expiration
- 2034-08-23
AI Technical Summary
Existing cell culture dishes cannot be operated in a closed environment during the liquid change process, resulting in inconvenience in operation and high risk of contamination, and the liquid is prone to splashing, which may damage cells.
A cell culture dish with a liquid inlet interface, a liquid inlet valve, a liquid outlet interface and a liquid outlet valve is designed. The liquid replacement operation is realized in a closed environment with the flow guide. It is connected to the syringe through the liquid inlet interface, and the flow guide is used to guide the flow. The liquid outlet port is fitted with the inner wall of the petri dish cover to prevent liquid from splashing.
It realizes cell liquid replacement operation in a closed environment, reducing the risk of contamination, prevents liquid splashing, avoids cell damage, and makes operation convenient and reduces inconvenience during liquid replacement.
Smart Images
Figure CN223189190U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technology in the field of cell preparation, in particular to a cell culture dish suitable for the liquid change culture of biological cells. Background Art
[0002] Cell culture technology is an important method to simulate the in-vivo environment in vitro so that cells can survive, grow, reproduce and maintain their main structures and functions. In the process of traditional manual preparation, high requirements of stable quality and sterility must be met in each operation link. In the current preparation process of cell liquid change culture, most use an open-top cell culture dish for liquid change, and a pipette is connected to a pipetting gun to aspirate and add liquid. This liquid change method is commonly used in cell preparation production; the cell culture dish in the prior art does not adopt an inlet interface, an inlet valve, an outlet interface and an outlet valve to realize the cell culture liquid change operation in a sealed environment after the culture dish body and the culture dish cover are buckled, which is not convenient for various cells that need liquid change culture; it does not adopt a flow guiding member to realize the flow guiding effect of the liquid, which is not convenient for operation; therefore, in view of this current situation, there is an urgent need to develop a cell culture dish suitable for the liquid change culture of biological cells to meet the actual use requirements. Content of the Utility Model
[0003] In view of this, aiming at the deficiencies existing in the prior art, the main purpose of the utility model is to provide a cell culture dish suitable for the liquid change culture of biological cells, which realizes the cell culture liquid change operation in a sealed environment after the culture dish body and the culture dish cover are buckled by adopting an inlet interface, an inlet valve, an outlet interface and an outlet valve, and is suitable for various cells that need liquid change culture; by adopting a flow guiding member to realize the flow guiding effect of the liquid, it is convenient for liquid inlet, the edge of the liquid outlet is fitted with the inner wall of the culture dish cover to prevent liquid splashing and avoid damage to cells at the same time.
[0004] To achieve the above purpose, the utility model adopts the following technical solutions:
[0005] A cell culture dish suitable for the liquid change culture of biological cells includes a culture dish body and a culture dish cover that are buckled together. The culture dish cover is provided with an inlet interface for connecting with a syringe. An inlet valve is movably arranged in the inlet interface. The lower end of the inlet interface is connected with a flow guiding member for guiding the flow. The flow guiding member faces the culture dish body. The lower end of the flow guiding member is provided with a liquid outlet, and the edge of the liquid outlet is fitted with the inner wall of the culture dish cover; an outlet interface for connecting with a syringe is arranged on the side wall of the culture dish body, and an outlet valve is movably arranged in the outlet interface.
[0006] As a preferred solution: the guide member is a guide tube, which is in the shape of an inverted cone and inclined five to ten degrees toward the inner wall of the culture dish. The edge of the liquid outlet is tangent to the inner wall of the culture dish cover.
[0007] As a preferred solution: the liquid inlet valve adopts a liquid inlet ball valve, which is rotatably arranged in the liquid inlet interface; the liquid outlet valve adopts a liquid outlet ball valve, which is rotatably arranged in the liquid outlet interface.
[0008] As a preferred solution: the liquid inlet ball valve is tightly connected to a liquid inlet switch, which is rotatably arranged on the side wall of the liquid inlet interface; the liquid outlet ball valve is tightly connected to a liquid outlet switch, which is rotatably arranged on the side wall of the liquid outlet interface.
[0009] As a preferred solution: the liquid inlet interface and the liquid outlet interface are both embedded with filter screens for filtering.
[0010] As a preferred solution, the outer side wall of the culture dish body is provided with a flange protruding outward along its circumference for easy grasping, and the front side of the flange is provided with convex teeth along its circumference for anti-slip effect.
[0011] As a preferred solution, the culture dish body and the culture dish cover are detachably tightly fitted together.
[0012] Compared with the prior art, the present invention has obvious advantages and beneficial effects. Specifically, it can be seen from the above technical scheme that by adopting the liquid inlet interface, the liquid inlet valve, the liquid outlet interface and the liquid outlet valve, the cell culture fluid exchange operation can be carried out in a closed environment after the culture dish body and the culture dish cover are buckled together, which is suitable for various cells that need to be cultured with fluid exchange; when the culture dish body and the culture dish cover are buckled together, the cell fluid exchange operation can be carried out, which effectively solves the technical problems of the inconvenience of operation and the high risk of contamination caused by fully opening the culture dish cover during the fluid exchange process; by adopting the guide member to achieve the diversion effect on the liquid, it is convenient for liquid to enter, and the edge of the liquid outlet is fitted with the inner wall of the culture dish cover to prevent liquid splashing, while also avoiding damage to the cells.
[0013] In order to more clearly illustrate the structural features and effects of the present invention, it is described in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 This is a schematic diagram of the three-dimensional structure of the cell culture dish of the present invention from a first perspective;
[0015] Figure 2 This is a schematic diagram of the three-dimensional structure of the cell culture dish of the present invention from a second perspective;
[0016] Figure 3This is a third - perspective three - dimensional structural schematic diagram of the cell culture dish of the present utility model;
[0017] Figure 4 This is a three - dimensional structural schematic diagram of the culture dish cover of the present utility model.
[0018] Explanation of the attached drawing reference numerals:
[0019] In the figure: 10, culture dish cover; 11, liquid inlet interface; 12, liquid inlet switch; 13, flow - guiding member; 14, liquid outlet; 20, culture dish body; 21, liquid outlet interface; 22, liquid outlet switch; 23, flange; 24, convex teeth. Specific embodiments
[0020] As shown in the present utility model Figures 1 to 4 A cell culture dish suitable for biological cell liquid - changing culture includes a culture dish body 20 and a culture dish cover 10 that are buckled together. Among them:
[0021] The culture dish cover 10 is provided with a liquid inlet interface 11 for connecting with a syringe. A liquid inlet valve is movably arranged in the liquid inlet interface 11. The lower end of the liquid inlet interface 11 is connected with a flow - guiding member 13 for guiding the flow. The flow - guiding member 13 faces the culture dish body 20. The lower end of the flow - guiding member 13 is provided with a liquid outlet 14. The edge of the liquid outlet 14 is in contact with the inner wall of the culture dish cover 10. A liquid outlet interface 21 for connecting with a syringe is provided on the side wall of the culture dish body 20. A liquid outlet valve is movably arranged in the liquid outlet interface 21.
[0022] When the culture dish body 20 and the culture dish cover 10 are buckled together, when sucking the waste liquid in the culture dish body 20, a disposable syringe is connected to the liquid outlet interface 21 of the culture dish body 20, and the ball valve is opened for suction. When adding fresh culture medium to the culture dish body 20, a disposable syringe is connected to the liquid inlet interface 11 of the culture dish cover 10, the liquid inlet valve is opened, and the culture medium is injected into the culture dish body 20. During the injection of the culture medium, the flow - guiding member 13 plays a role in guiding the flow of the culture medium.
[0023] By using the liquid inlet interface 11, liquid inlet valve, liquid outlet interface 21 and liquid outlet valve, the cell culture liquid - changing operation can be carried out in a sealed environment after the culture dish body 20 and the culture dish cover 10 are buckled together, which is suitable for various cells that need liquid - changing culture. When the culture dish body 20 and the culture dish cover 10 are buckled together, the cell liquid - changing operation can be carried out, effectively solving the technical problems of inconvenient operation and high pollution risk caused by completely opening the culture dish cover 10 during the liquid - changing process. By using the flow - guiding member 13 to achieve the guiding effect of the liquid, it is convenient for liquid inlet. The edge of the liquid outlet 14 is in contact with the inner wall of the culture dish cover 10 to prevent liquid splashing and avoid damage to cells at the same time.
[0024] It is convenient to pick up, operate and transfer. More importantly, it can better perform liquid replacement operations and reduce the risk of contamination during operation; it is applicable to liquid replacement operations for various cell cultures. Compared with the existing method of using a pipette to aspirate after opening the lid, replacing the cell culture liquid through a syringe without opening the lid has the advantages of reducing the risk of contamination and being able to fully suck out the waste liquid; secondly, this cell culture dish is also convenient for operation and transfer.
[0025] The flow guide member 13 is a flow guide tube, which is in the shape of an inverted cone, and the flow guide tube inclines five to ten degrees towards the inner wall of the culture dish body 20. The edge of the liquid outlet 14 is tangent to the inner wall of the culture dish lid 10; this prevents liquid from splashing when adding liquid.
[0026] The inlet valve uses an inlet ball valve, and the inlet ball valve is rotatably arranged in the inlet interface 11; the outlet valve uses an outlet ball valve, and the outlet ball valve is rotatably arranged in the outlet interface 21.
[0027] The inlet ball valve is firmly connected to an inlet switch 12, and the inlet switch 12 is rotatably arranged on the side wall of the inlet interface 11; the outlet ball valve is firmly connected to an outlet switch 22, and the outlet switch 22 is rotatably arranged on the side wall of the outlet interface 21.
[0028] By using the inlet switch 12 and the outlet switch 22, it is convenient to control the inlet and outlet of liquid, and the operation is convenient.
[0029] Filter meshes for filtering are embedded in both the inlet interface 11 and the outlet interface 21.
[0030] On the outer side wall of the culture dish body 20, a flange 23 for easy grasping is protrudingly provided along its circumference, and on the front side of the flange 23, convex teeth 24 for anti-slip are provided along its circumference.
[0031] By using the flange 23 and the convex teeth 24, it is convenient for the operator to grasp the culture dish body 20.
[0032] The culture dish body 20 and the culture dish lid 10 are detachably and tightly fitted and buckled; the culture dish body 20 and the culture dish lid 10 are each an integral body, which is convenient for opening and replacement.
[0033] The usage method and principle of this cell culture dish applicable to biological cell liquid replacement culture are as follows:
[0034] Fasten the culture dish body and the culture dish lid. When sucking the waste liquid in the culture dish body, connect a disposable syringe to the liquid outlet interface of the culture dish body, open the ball valve, and aspirate; when adding fresh culture medium to the culture dish body, connect a disposable syringe to the liquid inlet interface of the culture dish lid, open the inlet valve, and inject the culture medium into the culture dish body. During the injection of the culture medium, the flow guide member plays a role in guiding the culture medium.
[0035] The design focus of the present utility model is to achieve cell culture liquid replacement operation in a sealed environment after the culture dish body and the culture dish cover are buckled by adopting a liquid inlet interface, a liquid inlet valve, a liquid outlet interface and a liquid outlet valve, which is applicable to various cells that need liquid replacement culture; when the culture dish body and the culture dish cover are buckled, cell liquid replacement operation can be carried out, effectively solving the technical problems of inconvenient operation and high pollution risk caused by completely opening the culture dish cover during the liquid replacement process; by adopting a diversion member to achieve the diversion effect of the liquid, facilitating liquid inlet, and fitting the edge of the liquid outlet with the inner wall of the culture dish cover to prevent liquid splashing and avoid damage to cells at the same time.
[0036] The above are only preferred embodiments of the present utility model, and do not impose any limitation on the technical scope of the present utility model. Therefore, any minor modification, equivalent change and modification made to the above embodiments based on the technical essence of the present utility model still fall within the scope of the technical solution of the present utility model.
Claims
1. A cell culture dish suitable for culture of biological cells, characterized by: The invention comprises a culture dish body and a culture dish cover which are interlocked with each other. The culture dish cover is provided with a liquid inlet interface for connecting with a syringe, and a liquid inlet valve is movably provided in the liquid inlet interface. The lower end of the liquid inlet interface is connected to a flow guide member for guiding flow, and the flow guide member faces the culture dish body. The lower end of the flow guide member is provided with a liquid outlet, and the edge of the liquid outlet is in contact with the inner wall of the culture dish cover; the side wall of the culture dish body is provided with a liquid outlet interface for connecting with a syringe, and a liquid outlet valve is movably provided in the liquid outlet interface.
2. A cell culture dish suitable for biological cell culture according to claim 1, characterized in that: The guide member is a guide tube in the shape of an inverted cone. The guide tube is inclined five to ten degrees toward the inner wall of the culture dish, and the edge of the liquid outlet is tangent to the inner wall of the culture dish cover.
3. The cell culture dish suitable for biological cell culture according to claim 1, characterized in that: The liquid inlet valve adopts a liquid inlet ball valve, which is rotatably arranged in the liquid inlet interface; the liquid outlet valve adopts a liquid outlet ball valve, which is rotatably arranged in the liquid outlet interface.
4. A cell culture dish suitable for biological cell culture according to claim 3, characterized in that: The liquid inlet ball valve is tightly connected to a liquid inlet switch, which is rotatably arranged on the side wall of the liquid inlet interface; the liquid outlet ball valve is tightly connected to a liquid outlet switch, which is rotatably arranged on the side wall of the liquid outlet interface.
5. The cell culture dish suitable for biological cell culture according to claim 1, characterized in that: The liquid inlet interface and the liquid outlet interface are both embedded with filter screens for filtering.
6. The cell culture dish suitable for biological cell culture according to claim 1, characterized in that: The outer side wall of the culture dish body is provided with a flange protruding outward along its circumference for easy grasping, and the front side of the flange is provided with convex teeth for anti-slip along its circumference.
7. The cell culture dish suitable for biological cell culture according to claim 1, characterized in that: The culture dish body and the culture dish cover are detachably tightly fitted together.