DPPH double-bin reagent tube

Abstract

The utility model provides a DPPH double-bin reagent tube, which relates to the technical field of experimental instruments and comprises an ethanol accommodating bin, a DPPH reagent accommodating bin and a hollow tube between the ethanol accommodating bin and the DPPH reagent accommodating bin. The DPPH reagent containing bin comprises an upper cover and an attachment pipe connected to the lower end of the upper cover, and the tip end of the attachment pipe is attached to the sealing film in the gasket. By tearing off the snap type annular sealing strip and pressing the upper cover, the attachment tube punctures the sealing film, and then the reagent tube is reversed and shaken, so that the ethanol and the DPPH freeze-dried powder are mixed. The ethanol containing bin and the hollow pipe are connected in a sealed mode through a gasket, the upper cover and the hollow pipe are in hole-shaft interference fit, and the sealing performance is ensured. The ethanol containing bin is made of a transparent material, so that observation is facilitated, and the hollow pipe and the DPPH containing bin are made of non-transparent materials, so that the stability of the DPPH freeze-dried powder is kept. According to the utility model, the experimental process is simplified, the experimental accuracy and efficiency are improved, the health of experimenters is guaranteed, the waste of medicaments is reduced, and the stability of the medicaments is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental instruments, in particular to a DPPH double-compartment reagent tube. Background Art

[0002] DPPH, a stable free radical, is widely used in chemistry and biology, particularly in the determination of antioxidant activity. Traditional DPPH radical scavenging experiments typically require removing an appropriate amount of DPPH powder or solution from a can of reagent, mixing it with ethanol in a specific ratio based on experimental requirements, and then mixing it with the sample to be tested before measuring absorbance. However, this method has the following shortcomings:

[0003] Complex operations and difficulty in standardization: Each experiment requires precise weighing of DPPH powder or measurement of DPPH solution and mixing with ethanol. Furthermore, due to differences in operator performance, the mixing process can vary, affecting the consistency and reproducibility of experimental results. This process is not only time-consuming but also prone to human error.

[0004] Safety issues: DPPH powder and ethanol are both volatile chemicals. Improper handling may have an impact on human health and also pose a safety hazard.

[0005] Waste of resources: Once DPPH is dissolved in ethanol, its stability is poor, so it is generally required to be prepared and used immediately. Excess reagents may be generated during the preparation process, and DPPH reagents are prone to degradation during storage. To ensure the accuracy and consistency of the results, unused reagents must be discarded and re-prepared, which can easily lead to repeated waste of resources and pollute the environment. Utility Model Content

[0006] The purpose of the utility model is to provide a DPPH dual-compartment reagent tube, which integrates DPPH reagent and ethanol. The dual-compartment reagent tube can accommodate pre-installed DPPH reagent and ethanol in a standard ratio, which can be quickly mixed during the test, allowing users to quickly and easily complete DPPH free radical scavenging experiments while ensuring the reliability and consistency of the experimental results.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A DPPH double-compartment reagent tube, comprising an ethanol containing compartment and a DPPH reagent containing compartment, wherein the ethanol containing compartment and the DPPH reagent containing compartment are connected via a hollow tube;

[0009] A sealing membrane is provided in the passage between the ethanol holding chamber and the DPPH reagent holding chamber, and the sealing membrane isolates the ethanol holding chamber from the cavity in the central tube;

[0010] The DPPH reagent containing chamber includes an upper cover and an attachment tube connected to the lower end of the upper cover. The upper cover and the hollow tube are connected by a breakable annular seal. The lower end of the attachment tube is close to the sealing film and the lower end of the attachment tube is a pointed end.

[0011] To prepare the DPPH reagent for testing, simply tear off the snap-off ring seal, press the top cap, and allow the tip of the attached tube to pierce the seal. Press the top cap tightly against the hollow tube, invert, and shake the DPPH dual-compartment reagent tube to allow the ethanol in the ethanol reservoir to flow into the attached tube and mix thoroughly with the DPPH freeze-dried powder. Reset the DPPH dual-compartment reagent tube to complete the preparation of the DPPH reagent. Preparation is quick and easy; there's no contamination during the preparation process, ensuring the health of test personnel. The resulting DPPH reagent has a more standardized ratio and minimizes errors.

[0012] Furthermore, the ethanol storage chamber and the hollow tube are sealed by a gasket, the gasket having a central hole connecting the ethanol storage chamber and the hollow tube, and the sealing film is disposed in the central hole. The gasket sealing connection structure can ensure the sealing of the ethanol storage chamber and prevent ethanol from volatilizing.

[0013] Furthermore: the gasket is made of plastic, and the sealing film is made of the same material as the gasket.

[0014] Furthermore, the outer diameter of the upper cover and the inner diameter of the hollow tube form an interference fit. When the attachment tube is inserted into the ethanol storage chamber, the upper cover can be pressed into the inner diameter of the hollow tube to form an interference fit, achieving a sealed connection and preventing leakage of the drug during shaking and mixing.

[0015] Furthermore, a cavity with an open lower end is provided in the attachment tube. The DPPH freeze-dried powder is attached to the inner wall of the attachment tube, and the DPPH freeze-dried powder is kept in a good light-shielding environment during production and transportation.

[0016] Furthermore, the attachment tube has a plurality of through-holes formed in its wall. When the attachment tube pierces the sealing membrane and enters the ethanol storage chamber, during shaking, the ethanol solvent can enter the cavity of the attachment tube more quickly and fully through the through-holes, achieving more complete mixing with the DPPH freeze-dried powder and reducing the residual DPPH freeze-dried powder on the inner wall of the attachment tube.

[0017] Furthermore, the ethanol storage chamber is made of transparent material, while the hollow tube and DPPH storage chamber are made of opaque material. The transparent material facilitates observation of the ethanol liquid level and color changes during the test; the opaque material keeps the DPPH freeze-dried powder in good light-shielding conditions to prevent its degradation.

[0018] Furthermore: the material of the ethanol holding chamber is polypropylene, and the material of the hollow tube and the DPPH holding chamber are both high-density polyethylene.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. Simplify the experimental process: By integrating pre-installed DPPH reagent and ethanol, the experimental process is simplified. Users only need simple operations to complete the preparation of DPPH reagent, which improves experimental efficiency.

[0021] 2. Improve experimental accuracy: Using a fixed ratio of DPPH freeze-dried powder and ethanol reduces human operation errors and ensures the consistency and reliability of experimental results.

[0022] 3. Ensure the health of experimental personnel and reduce drug waste: There is no need for direct contact with drugs during the preparation process, which effectively avoids drug contamination and protects the health of experimental personnel; at the same time, it avoids the excess drugs that may be produced in traditional methods and reduces resource waste. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural diagram of the utility model;

[0024] In the picture:

[0025] 1. Upper cover; 2. Attachment tube; 2.1. Through hole; 2.2. Tip; 3. Hollow tube; 4. Gasket; 5. Ethanol container; 6. Sealing film; 7. Breakaway ring seal. DETAILED DESCRIPTION

[0026] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0027] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating positions or relationships, are based on the positions or relationships shown in the accompanying drawings and are intended solely to facilitate the description of this utility model and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0028] like Figure 1 As shown: a DPPH double-compartment reagent tube, including an ethanol containing compartment 5 and a DPPH reagent containing compartment, wherein the ethanol containing compartment 5 and the DPPH reagent containing compartment are connected via a hollow tube 3;

[0029] The ethanol storage chamber 5 and the hollow tube 3 are sealed by a gasket 4. The gasket 4 defines a central hole connecting the ethanol storage chamber 5 and the hollow tube 4. A sealing membrane 6 is disposed in the central hole to isolate the ethanol storage chamber 5 from the inner cavity of the central tube 3. The gasket 4 is made of plastic, and the sealing membrane 6 is made of the same material as the gasket 4. In another embodiment, the gasket 4 can be made of rubber. In another embodiment, the sealing membrane 6 can be made of aluminum film.

[0030] The DPPH reagent holding chamber includes an upper cover 1 and an attachment tube 2 connected to the lower end of the upper cover 1. The upper cover 1 and the hollow tube 3 are connected by a breakable annular seal 7. The lower end of the attachment tube 2 is close to the sealing film 6, and the lower end of the attachment tube 2 is a tip 2.2.

[0031] When preparing the DPPH reagent for an experiment, simply tear off the breakaway annular sealing strip, press the upper cover 1, and allow the tip 2.2 of the attached tube 2 to pierce the sealing membrane 6. Press the upper cover 1 tightly onto the hollow tube 3, invert and shake the DPPH double-compartment reagent tube to allow the ethanol in the ethanol holding compartment 5 to flow into the attached tube 2 and fully mix with the DPPH freeze-dried powder on the attached tube 2. Then, reset the DPPH double-compartment reagent tube to complete the preparation of the DPPH reagent. The preparation operation is convenient and quick; there is no drug contamination during the preparation process, ensuring the health of the test personnel; the prepared DPPH reagent has a more standardized ratio and a small error; it can be prepared and used immediately according to the test requirements, without any waste.

[0032] The ethanol storage chamber 5 and the hollow tube 3 are sealed together by a gasket 4, which has a through hole 2.1 connecting the ethanol storage chamber 5 and the hollow tube 3. The gasket 4 ensures the sealing of the ethanol storage chamber 5 and prevents ethanol volatilization. The outer diameter of the upper cover 1 and the inner diameter of the hollow tube 3 have a hole-axis interference fit. When the attachment tube 2 is inserted into the ethanol storage chamber 5, the upper cover 1 can be pressed into the inner diameter of the hollow tube 3 to form an interference fit with the hollow tube 3, achieving a sealed connection and preventing leakage of the agent during the shaking and mixing process.

[0033] A cavity with an opening at the lower end is provided in the attachment tube 2. The outer diameter of the attachment tube 2 is the same as the diameter of the center hole of the gasket 4, and the size relationship is an interference fit relationship. The DPPH freeze-dried powder is attached to the inner wall of the attachment tube 2, and the DPPH freeze-dried powder is kept in a good light-shielding environment during the production and transportation process. A plurality of through holes 2.1 are provided on the tube wall of the attachment tube 2. When the attachment tube 2 pierces the sealing membrane 6 and penetrates the ethanol containing chamber 5, during the shaking process, the ethanol solvent can enter the cavity in the attachment tube 2 faster and more fully from the through holes 2.1, thereby achieving more complete mixing with the DPPH freeze-dried powder and reducing the residue of DPPH freeze-dried powder on the inner wall of the attachment tube 2.

[0034] The ethanol storage chamber 5 is made of transparent material, while the hollow tube 3 and DPPH storage chamber are made of opaque material. The transparent material facilitates observation of the ethanol liquid level and color changes during testing; the opaque material keeps the DPPH freeze-dried powder in good light-shielding conditions to prevent degradation. The storage chamber is made of polypropylene, while the hollow tube 3 and DPPH storage chamber are both made of high-density polyethylene.

[0035] The working principle of this utility model is as follows:

[0036] The user first tears off the breakable annular seal 7 between the upper cover 1 and the hollow tube 3 ; then presses the upper cover 1 to allow the tip 2 . 2 of the attachment tube 2 to pierce the sealing membrane 6 .

[0037] Press the upper cover 1 tightly onto the hollow tube 3, turn it upside down and shake the DPPH dual-compartment reagent tube. The upper cover 1 and the hollow tube 3 are designed with an interference fit between the hole and the axis to ensure that the reagent will not leak during the mixing process.

[0038] During the shaking process, ethanol enters the interior of the attachment tube 2 through the through-holes 2.1 in the wall of the attachment tube 2 and is thoroughly mixed with the DPPH freeze-dried powder. The through-holes 2.1 in the wall of the attachment tube 2 facilitate faster and more complete entry of ethanol into the cavity of the attachment tube 2, achieving more complete mixing with the DPPH freeze-dried powder.

[0039] After mixing is complete, the DPPH dual-compartment reagent tube is reset. The transparent ethanol holding compartment 5 material makes it easy to observe the liquid level and color changes of the ethanol.

[0040] The above embodiments are intended only to illustrate the technical concepts and features of the present invention. Their purpose is to enable those familiar with the art to understand the contents of the present invention and implement them accordingly. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications based on the spirit of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A DPPH dual-compartment reagent tube, characterized in that: It includes an ethanol containing chamber and a DPPH reagent containing chamber, and the ethanol containing chamber and the DPPH reagent containing chamber are connected by a hollow tube; A sealing membrane is provided in the passage between the ethanol holding chamber and the DPPH reagent holding chamber, and the sealing membrane isolates the ethanol holding chamber from the cavity in the central tube; The DPPH reagent containing chamber includes an upper cover and an attachment tube connected to the lower end of the upper cover. The upper cover and the hollow tube are connected by a breakable annular seal. The lower end of the attachment tube is close to the sealing film and the lower end of the attachment tube is a pointed end.

2. A DPPH dual-compartment reagent tube according to claim 1, characterized in that: The ethanol containing chamber and the hollow tube are sealed and connected via a gasket. The gasket is provided with a central hole communicating with the ethanol containing chamber and the hollow tube, and a sealing film is arranged in the central hole.

3. A DPPH dual-compartment reagent tube according to claim 2, characterized in that: The gasket is made of plastic.

4. A DPPH dual-compartment reagent tube according to claim 2, characterized in that: The attachment tube is provided with a cavity with an open lower end. The outer diameter of the attachment tube is the same as the diameter of the center hole of the gasket, and the size relationship is an interference fit between the hole and the axis.

5. A DPPH dual-compartment reagent tube according to claim 1, characterized in that: The dimensional relationship between the outer diameter of the upper cover and the inner diameter of the hollow tube is an interference fit between the hole and the shaft.

6. A DPPH dual-compartment reagent tube according to claim 5, characterized in that: A plurality of through holes are provided on the wall of the attachment tube.

7. A DPPH dual-compartment reagent tube according to claim 1, characterized in that: The ethanol containing chamber is made of transparent material, and the hollow tube and the DPPH containing chamber are made of opaque material.

8. A DPPH dual-compartment reagent tube according to claim 7, characterized in that: The material of the ethanol holding chamber is polypropylene, and the material of the hollow tube and DPPH holding chamber are both high-density polyethylene.

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