Tunable multi-wavelength fluorescent protein colony observing and counting device
Through the tunable multi-wavelength fluorescent protein colony observation device, the tunable laser and high-sensitivity CMOS sensor are used to solve the problem that existing devices cannot accurately adjust the light source, and the flexibility and accuracy of multi-wavelength fluorescent protein observation is achieved, improving the image acquisition quality and user experience.
Patent Information
- Application Number
- CN202422412702.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-08
- Publication Date
- 2025-08-15
- Estimated Expiration
- 2034-10-08
AI Technical Summary
The existing multi-wavelength fluorescent protein colony observation device cannot accurately regulate according to the excitation wavelength requirements of different fluorescent proteins, which limits its application in the observation of multiple fluorescent proteins.
The tunable multi-wavelength fluorescent protein colony observation and counting device is adopted, combined with a tunable laser, a low-pass optical filter and a high-sensitivity CMOS sensor, to achieve flexible adjustment of light sources and real-time image processing, and to improve the accuracy and applicability of observations.
Accurate light source adjustment in a wide wavelength range is achieved, background noise is reduced, image acquisition quality is improved, and the flexibility and user experience of the observation device are enhanced, ensuring safety and efficiency.
Smart Images
Figure CN223229441U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of fluorescent protein observation, in particular to a tunable multi-wavelength fluorescent protein colony observation and counting device. Background Art
[0002] Traditional blue light gel cutting instruments, as a commonly used fluorescence observation tool in biological experiments, mainly rely on a single blue light excitation light source, which limits their application in the observation of multiple fluorescent proteins. With the rapid development of molecular biology technology, a variety of fluorescent proteins have been widely used in fields such as cell labeling and protein interaction research. These fluorescent proteins often require light of different wavelengths for effective excitation. Therefore, it is particularly important to develop an observation device that can flexibly adjust the excitation wavelength and take into account both direct observation and digital imaging processing.
[0003] In response to the shortcomings of the existing technology, the present invention aims to provide an intelligent multi-wavelength tunable fluorescent protein observation device, which can be precisely adjusted according to the excitation wavelength requirements of different fluorescent proteins, and through modular design, flexible switching of observation modes can be achieved to meet the needs of scientific research observation and experimental results recording in multiple scenarios. Utility Model Content
[0004] The technical problem to be solved by the present invention is that the existing technology has the disadvantage that the multi-wavelength fluorescent protein colony observation and counting device cannot be accurately adjusted according to the excitation wavelength requirements of different fluorescent proteins. For this reason, we propose a tunable multi-wavelength fluorescent protein colony observation and counting device.
[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical solution: a tunable multi-wavelength fluorescent protein colony observation and counting device, comprising an observation chamber, two observation windows are provided on the top of the observation chamber, an LCD display, a counting button and a power supply are provided at the front end of the observation chamber, a placement module is installed inside the observation chamber through a slide rail, a positioning notch is provided on the top of the placement module, a low-pass optical filter is provided inside the positioning notch, a CMOS sensor is provided on the top of the low-pass optical filter, a culture dish is installed on the top of the placement module above the positioning notch, a single-chip microcomputer is installed inside the observation chamber, and the LCD display, counting button and power supply are electrically connected to the single-chip microcomputer.
[0006] Preferably, a tunable laser is provided on both sides of the top of the placement module above the positioning notch, the inclination angle of the tunable laser is thirty-nine degrees, and the bottom of the single chip microcomputer is provided with module connection contacts and a card slot.
[0007] Preferably, the observation window is a specially made amber filter.
[0008] Preferably, an LED light board is installed inside the observation chamber, an image collector is installed at the bottom of the LED light board, and a vertical plane of the center line of the LED light board intersects with the center line of the positioning notch.
[0009] Preferably, the diameter of the positioning notch ranges from 40 mm to 100 mm, and the depth ranges from 1.5 mm to 2.5 mm.
[0010] Preferably, a baffle is fixed to one end of the placement module, and a handle is fixed to one side of the baffle.
[0011] Preferably, a micro-touch switch is provided on a side of the observation chamber close to the baffle, and the micro-touch switch is electrically connected to the single-chip microcomputer.
[0012] Preferably, the model of the single chip microcomputer is AVR series.
[0013] Technical effects and advantages of this utility model:
[0014] The innovative value of this utility model is mainly reflected in the following aspects:
[0015] Tunable light source: The device uses advanced tunable laser technology to achieve precise adjustment over a wide wavelength range, providing a more flexible and efficient light source option for the excitation and observation of fluorescent proteins. This innovation significantly improves the accuracy and applicability of observations.
[0016] Optimization and upgrade of the optical system: By introducing higher-quality low-pass optical filters and more sensitive CMOS sensors, the device has achieved significant improvements in the optical system. This not only reduces background noise but also improves the quality of image acquisition, allowing users to obtain clearer and more accurate fluorescence images.
[0017] Breakthroughs in intelligence and automation: The device has made significant progress in automation and intelligence. By developing automatic colony counting and morphological analysis software, and introducing machine learning algorithms for intelligent identification and classification, the device has greatly reduced manual intervention and improved the accuracy and efficiency of observation. This innovation enables users to complete complex observation tasks more easily and quickly.
[0018] Significantly improved user experience: The device has been optimized in terms of the human-computer interaction interface, operating procedures, and real-time preview function, significantly improving the user experience. This allows users to use the device more conveniently and intuitively for observation and analysis of fluorescent proteins.
[0019] Strong safety: Safety performance is fully considered during the design and manufacturing process of the device to ensure that all electronic and optical components meet safety standards. This innovation allows users to conduct fluorescent protein observation experiments with greater confidence and peace of mind during use. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the main structure of the utility model;
[0021] Figure 2 This is a schematic diagram of the internal cross-sectional structure of the present invention;
[0022] Figure 3 This is a schematic diagram of the placement module structure of the present utility model;
[0023] Figure 4 This is a schematic diagram of the split structure of the placement module of the present utility model;
[0024] Figure 5 This is a schematic diagram of the disassembled structure of the micro-touch switch of the present invention.
[0025] Legend: 1. Observation chamber; 2. Observation window; 3. LCD display; 4. Counting button; 5. Power supply; 6. Module placement; 7. Positioning notch; 8. Low-pass optical filter; 9. CMOS sensor; 10. Petri dish; 11. Single-chip microcomputer; 12. Tunable laser; 13. Module connection contacts and slot; 14. LED light board; 15. Baffle; 16. Handle; 17. Micro-touch switch; 18. Image collector. DETAILED DESCRIPTION
[0026] The present invention will now be described in further detail with reference to the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show components related to the present invention.
[0027] Reference Figure 1-Figure 5As shown, the utility model provides a technical solution: a tunable multi-wavelength fluorescent protein colony observation and counting device, comprising an observation chamber 1, two observation windows 2 are provided on the top of the observation chamber 1, an LCD display 3, a counting button 4 and a power supply 5 are provided at the front end of the observation chamber 1, a placement module 6 is installed inside the observation chamber 1 through a slide rail, a positioning notch 7 is provided on the top of the placement module 6, a low-pass optical filter 8 is provided inside the positioning notch 7, a CMOS sensor 9 is provided on the top of the low-pass optical filter 8, a culture dish 10 is installed on the top of the placement module 6 above the positioning notch 7, a single-chip microcomputer 11 is installed inside the observation chamber 1, the LCD display 3, the counting button 4 and the power supply 5 are electrically connected to the single-chip microcomputer 11, a baffle 15 is fixed at one end of the placement module 6, and a handle 16 is fixed on one side of the baffle 15. Through the slide rail design, the placement module 6 can be horizontally moved in and out of the body, so that During user operation, the culture dish 10 is placed above the positioning slot 7, and then the low-pass optical filter 8 is placed inside the culture dish 10. The use of higher quality low-pass optical filter 8 and higher sensitivity CMOS sensor 9 enables the device to achieve significant improvement in the optical system. The low-pass optical filter 8 can effectively filter out stray light and improve the observation quality. The CMOS sensor 9 is closely connected to the computer system to achieve real-time transmission and processing of images, which not only reduces background noise, but also improves the quality of image acquisition. The observation chamber 1 is combined with the observation window 2 to provide the user with a clear observation field of view, allowing the user to obtain clearer and more accurate fluorescence images. Combined with advanced tunable laser 12 technology, precise adjustment within a wide wavelength range is achieved, providing a more flexible and efficient light source selection for the excitation and observation of fluorescent proteins, thereby improving the accuracy and applicability of observation.
[0028] Reference Figure 3 and Figure 4 As shown, in this embodiment: the top of the placement module 6 is located on both sides above the positioning notch 7 and is provided with a tunable laser 12, the inclination angle of the tunable laser 12 is thirty-nine degrees, and the bottom of the single-chip computer 11 is provided with module connection contacts and a card slot 13. By setting the tunable laser 12, its default wavelength light is λ = 440 ~ 490nm, and the DFB distributed feedback laser is combined with the current tuning technology to achieve wavelength adjustment in a wide range, which is suitable for the excitation and observation of fluorescent proteins, and the inclination angle of the tunable laser 12 is designed to be 39° to ensure that the light can be evenly irradiated on the culture dish 10.
[0029] Reference Figure 1 、 Figure 2 and Figure 5 As shown, in this embodiment: the observation window 2 is a special amber filter. By using the special amber filter of the observation window 2, it can prevent the damage of light of special wavelengths to the eyes, and at the same time facilitate the observation of the bacterial colony morphology.
[0030] Reference Figure 2 As shown, in this embodiment: an LED light board 14 is installed inside the observation chamber 1, an image collector 18 is installed at the bottom of the LED light board 14, and the vertical plane of the center line of the LED light board 14 intersects with the center line of the positioning slot 7. By designing the vertical plane of the center line of the LED light board 14 to intersect with the center line of the positioning slot 7, the best lighting effect can be achieved.
[0031] Reference Figure 4 As shown, in this embodiment: the diameter range of the positioning notch 7 is 40 mm-100 mm, and the depth range is 1.5 mm-2.5 mm, which can ensure that the positioning notch 7 of the device has a specific diameter and depth range, thereby adapting to culture dishes 10 of different sizes.
[0032] Reference Figure 5 As shown, in this embodiment: a micro-touch switch 17 is provided on one side of the observation chamber 1 close to the baffle 15, and the micro-touch switch 17 is electrically connected to the single-chip computer 11, which can realize intelligent control and safety protection.
[0033] Reference Figure 2 As shown, in this embodiment: the model of the single chip microcomputer 11 is AVR series. By adopting the AVR series single chip microcomputer 11, the performance is stable and reliable.
[0034] Working principle: Through the slide rail design, the user can make the placement module 6 move in and out of the body horizontally, which is convenient for user operation. The culture dish 10 is placed above the positioning slot 7, and then the low-pass optical filter 8 is placed inside the culture dish 10. The use of higher quality low-pass optical filter 8 and higher sensitivity CMOS sensor 9 has significantly improved the optical system of the device. The low-pass optical filter 8 can effectively filter out stray light and improve the observation quality. The CMOS sensor 9 is closely connected with the computer system to realize real-time transmission and processing of images, which not only reduces background noise but also improves the quality of image acquisition. The observation chamber 1 is combined with the observation window 2 to provide the user with a clear observation field of view, allowing the user to obtain clearer and more accurate fluorescence images. Combined with the advanced tunable laser 12 technology, precise adjustment within a wide wavelength range is achieved, providing a more flexible and efficient light source selection for the excitation and observation of fluorescent proteins, thereby improving the accuracy and applicability of observation. By setting the tunable laser 12, its default wavelength light is λ=
[0035] 440~490nm, using DFB distributed feedback laser combined with current tuning technology to achieve wavelength adjustment in a wide range, suitable for the excitation and observation of fluorescent proteins, and the inclination angle of the tunable laser 12 is designed to be 39° to ensure that the light can be evenly irradiated on the culture dish 10, by using a special amber filter for the observation window 2 to prevent damage to the eyes caused by light in a special wavelength band, while facilitating the observation of colony morphology, by designing the vertical plane of the center line of the LED light board 14 to intersect with the center line of the positioning slot 7, the best lighting effect can be achieved, and the positioning slot 7 of the device can be ensured to have a specific diameter and depth range, so as to adapt to culture dishes 10 of different sizes, and can achieve intelligent control and safety protection. By adopting the AVR series single-chip microcomputer 11, the performance is stable and reliable.
[0036] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A tunable multi-wavelength fluorescent protein colony observation and counting device, comprising an observation chamber (1), characterized in that: The top of the observation chamber (1) is provided with two observation windows (2), the front end of the observation chamber (1) is provided with an LCD display screen (3), a counting button (4) and a power supply (5), the interior of the observation chamber (1) is provided with a placement module (6) via a slide rail, the top of the placement module (6) is provided with a positioning notch (7), the interior of the positioning notch (7) is provided with a low-pass optical filter (8), the top of the low-pass optical filter (8) is provided with a CMOS sensor (9), the top of the placement module (6) is provided with a culture dish (10) above the positioning notch (7), the interior of the observation chamber (1) is provided with a single-chip microcomputer (11), and the LCD display screen (3), the counting button (4) and the power supply (5) are electrically connected to the single-chip microcomputer (11).
2. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: Tunable lasers (12) are provided on both sides of the top of the placement module (6) above the positioning notch (7), and the tilt angle of the tunable lasers (12) is thirty-nine degrees. The bottom of the single-chip computer (11) is provided with module connection contacts and a card slot (13).
3. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: The observation window (2) is a specially made amber filter.
4. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: An LED light board (14) is installed inside the observation chamber (1), an image collector (18) is installed at the bottom of the LED light board (14), and a vertical plane of the center line of the LED light board (14) intersects with the center line of the positioning notch (7).
5. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: The diameter of the positioning notch (7) ranges from 40 mm to 100 mm, and the depth ranges from 1.5 mm to 2.5 mm.
6. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: A baffle (15) is fixed to one end of the placement module (6), and a handle (16) is fixed to one side of the baffle (15).
7. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: A micro-touch switch (17) is provided on one side of the observation chamber (1) close to the baffle (15), and the micro-touch switch (17) is electrically connected to the single-chip computer (11).
8. A tunable multi-wavelength fluorescent protein colony observation and counting device according to claim 1, characterized in that: The model of the single chip microcomputer (11) is AVR series.