Cell cryopreservation bag with cell sieve function

Abstract

The utility model relates to the technical field of cell cryopreservation bags, and discloses a cell cryopreservation bag with a cell sieve function, which comprises a bag body, a cell sieve and an anti-blocking mechanism, a storage cavity used for storing cells is formed in the bag body, and a feeding port of the storage cavity is connected with the output end of the cell sieve; the anti-blocking mechanism comprises a telescopic pipe, the telescopic pipe is of a telescopic structure with one end sealed, the other end of the telescopic pipe is connected with a discharging port of the material storage cavity, and the telescopic pipe is used for conveying gas stored in the telescopic pipe into the material storage cavity or sucking gas in the material storage cavity into the telescopic pipe. The cell cryopreservation bag with the cell sieve function is convenient for assisting in dredging the sieve, and the efficiency of cells passing through the cell sieve is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell freezing bags, in particular to a cell freezing bag with a cell screening function. Background Art

[0002] Cell cryopreservation is a continuation of the cell culture process. It uses cryopreservation technology and corresponding equipment or devices to store cells at low temperatures in liquid nitrogen to put the cells into a dormant and detached growth state and preserve the cell characteristics. When these cells are needed, the frozen cells are revived. Currently, frozen cells are usually packaged in cryopreservation bags and used during resuscitation.

[0003] When cells are encapsulated in a cryopreservation bag, the cell population is screened through a cell sieve to effectively improve the relative purity of the cells collected in the cryopreservation bag. Currently, the cell population is first screened through a vessel, and then the cells screened in the vessel are placed in a cryopreservation bag. The cell sieve can be directly connected to the cryopreservation bag to reduce the intermediate operation steps and improve efficiency. During screening, the screen of the cell sieve is easily clogged, thereby reducing the efficiency of cells passing through the cell sieve. However, the current cryopreservation bags are not convenient for assisting in unblocking the screen, and cannot improve the situation where the efficiency of cells passing through the cell sieve is reduced after the cell sieve is clogged. Utility Model Content

[0004] The utility model aims to provide a cell freezing bag with a cell sieve function, which is convenient for assisting in dredging the sieve and effectively improving the efficiency of cells passing through the cell sieve.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] A cell freezing bag with a cell sieve function is designed, comprising a bag body, a cell sieve and an anti-blocking mechanism;

[0007] A storage cavity for storing cells is provided inside the bag body, and a feed port of the storage cavity is connected to an output end of the cell sieve;

[0008] The anti-blocking mechanism includes a telescopic tube, which is a telescopic structure with one end sealed. The other end of the telescopic tube is connected to the discharge port of the storage chamber, and is used to transport the gas stored in the internal telescopic tube to the storage chamber or to absorb the gas in the storage chamber into the telescopic tube.

[0009] Optionally, the cell sieve includes a hot melt tube, a sieve body and a sieve mesh, one end of the hot melt tube is detachably connected to the output end of the sieve body, and the other end is connected to the feed port of the storage chamber, and the end of the sieve body away from the hot melt tube is detachably connected to the sieve mesh through an opened groove.

[0010] Optionally, the anti-blocking mechanism further includes a blocking rubber pad, which is fixedly connected to the end face of the telescopic tube away from the material storage cavity.

[0011] Optionally, the anti-blocking mechanism further includes a protective cover, which is detachably connected to the end of the telescopic tube and is used to cover the blocking rubber pad.

[0012] Optionally, the bag body includes a containing bag and a protective bag, the storage cavity is opened in the containing bag, a buffer cavity is opened inside the protective bag, and the containing bag is arranged in the buffer cavity.

[0013] Optionally, the bag body further comprises a cotton block, the cotton block is fixedly connected to the inner wall of the buffer cavity, the cotton block abuts against the containing bag, and the cotton block is used to space the containing bag from the inner wall of the buffer cavity.

[0014] The utility model provides a cell freezing bag with a cell screening function, which has the following beneficial effects:

[0015] The cell freezing bag with a cell sieve function screens the cells entering the bag body through the cell sieve, and obtains a cell population with relatively high purity entering the bag body, and stores the screened cell population through the storage cavity. In conjunction with the function of the telescopic tube, when the cell sieve is blocked during the screening process or the cell passing efficiency is low, the telescopic tube is pressed by hand to discharge the gas in the telescopic tube, and then the gas enters the storage cavity and reaches the cell sieve. When the hand releases the telescopic tube, the telescopic tube expands itself, so that the gas at the cell sieve passes through the sieve hole and then enters the telescopic tube from the storage cavity. A small amount of airflow disturbance is used to assist in clearing the cell sieve, effectively avoiding blockage of the cell sieve and improving the efficiency of cells passing through the cell sieve. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the explosion of the cell freezing bag with cell sieve function in the utility model;

[0017] Figure 2 This is a three-dimensional structural diagram of a cell freezing bag with a cell sieve function in the present invention;

[0018] Figure 3 This is a schematic structural diagram of a cross-section of the bag body of the present invention.

[0019] In the figure: 10, bag body; 101, storage cavity; 102, containing bag; 103, protective bag; 104, cotton block; 20, cell sieve; 201, hot melt tube; 202, sieve body; 203, sieve; 30, anti-blocking mechanism; 301, telescopic tube; 302, blocking rubber pad; 303, protective cover. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] See also Figures 1 to 3 The utility model provides a technical solution: a cell freezing bag with a cell sieve function, comprising a bag body 10, a cell sieve 20 and an anti-blocking mechanism 30;

[0022] The bag body 10 is provided with a storage cavity 101 for storing cells, and the feed port of the storage cavity 101 is connected to the output end of the cell sieve 20;

[0023] The anti-blocking mechanism 30 includes a telescopic tube 301, which is a telescopic structure with one end sealed. The other end of the telescopic tube 301 is connected to the discharge port of the storage chamber 101, and is used to transport the gas stored in the telescopic tube 301 into the storage chamber 101 or to absorb the gas in the storage chamber 101 into the telescopic tube 301.

[0024] The storage cavity 101 opened by the bag body 10 is used to store the screened cells. The cell screen 20 is used to screen the cell population and improve the relative purity of the cells entering the storage cavity 101.

[0025] The telescopic tube 301 has a certain elastic force, or may not have elastic force. By manually pressing and then manually stretching, the telescopic tube 301 can transport gas to the storage chamber 101 or inhale gas. Through the compression and expansion of the telescopic tube 301, a small amount of airflow disturbance can be generated to assist in clearing the cell screen 20 and prevent the cell screen 20 from being blocked.

[0026] In this embodiment, as a preferred embodiment, the cell sieve 20 includes a hot melt tube 201, a sieve body 202, and a screen 203. One end of the hot melt tube 201 is detachably connected to the output end of the sieve body 202, and the other end is connected to the feed port of the storage chamber 101. The end of the sieve body 202 away from the hot melt tube 201 is detachably connected to the screen 203 through a groove.

[0027] Through the setting of the hot melt tube 201, when the screening of the cell population is completed, the hot melt tube 201 is directly sealed by hot melting, which reduces the trouble of sealing operation. At the same time, the sieve body 202 can be separated from the connection part of the hot melt tube 201, and the sieve body 202 can be disassembled and reused after cleaning. The screen 203 is used to screen the cell population. Through the connection between the screen 203 and the sieve body 202, the screen 203 can be disassembled and replaced to avoid cross contamination.

[0028] In this embodiment, as a preferred solution, the anti-blocking mechanism 30 further includes a blocking rubber pad 302, which is fixedly connected to the end surface of the telescopic tube 301 away from the storage chamber 101;

[0029] The telescopic tube 301 can be sealed by the plugging rubber pad 302 . When the cell colony in the storage cavity 101 needs to be taken out, the plugging rubber pad 302 can be opened to take out the cell colony in the storage cavity 101 .

[0030] In this embodiment, as a preferred solution, the anti-blocking mechanism 30 also includes a protective cover 303, which is detachably connected to the end of the telescopic tube 301 and is used to cover the blocking rubber pad 302. The protective cover 303 can protect the blocking rubber pad 302 to prevent the blocking rubber pad 302 from being contaminated when the telescopic tube 301 is pressed. It also prevents dust from accumulating on the blocking rubber pad 302 and contaminating the blocking rubber pad 302, and prevents the blocking rubber pad 302 from being accidentally damaged and causing the cell population in the storage chamber 101 to leak.

[0031] In this embodiment, as a preferred solution, the bag body 10 includes a containing bag 102 and a protective bag 103. The storage cavity 101 is opened in the containing bag 102. A buffer cavity is opened inside the protective bag 103. The containing bag 102 is set in the buffer cavity.

[0032] Through the connection between the protective bag 103 and the containing bag 102, the containing bag 102 is set in the buffer cavity. When placed in the freezer for frozen storage, the protective bag 103 first contacts the inner wall of the freezer. After the protective bag 103 exchanges heat and cools down, the buffer cavity inside it cools down. Then, the air in the buffer cavity contacts the containing bag 102 for heat exchange, so that the containing bag 102 gradually cools down and the cooling speed is slowed down to prevent the containing bag 102 from cooling down too quickly and damaging the cell population contained therein. At the same time, it also prevents the bag surface of the containing bag 102 from directly contacting the inner wall of the freezer to prevent direct contact from causing the freezing speed to be too fast.

[0033] In this embodiment, as a preferred solution, the bag body 10 further includes a cotton block 104, which is fixedly connected to the inner wall of the buffer cavity and abuts against the receiving bag 102. The cotton block 104 is used to space the receiving bag 102 from the inner wall of the buffer cavity.

[0034] The setting of the cotton block 104 can assist in positioning the containing bag 102, preventing the containing bag 102 from contacting the inner wall of the protective bag 103 due to the weight of the internal liquid, thereby affecting the buffer chamber's effect of slowing down the cooling rate, and also preventing the containing bag 102 from shaking and accidentally leaking liquid.

[0035] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell freezing bag with a cell screening function, characterized by: It comprises a bag body (10), a cell sieve (20) and an anti-blocking mechanism (30); The bag body (10) is provided with a storage cavity (101) for storing cells, and the feed port of the storage cavity (101) is connected to the output end of the cell sieve (20); The anti-blocking mechanism (30) comprises a telescopic tube (301), wherein the telescopic tube (301) is a telescopic structure with one end sealed, and the other end of the telescopic tube (301) is connected to the discharge port of the storage chamber (101), and is used to transport the gas stored in the telescopic tube (301) into the storage chamber (101) or to absorb the gas in the storage chamber (101) into the telescopic tube (301).

2. The cell freezing bag with cell screening function according to claim 1, characterized in that: The cell sieve (20) comprises a hot melt tube (201), a sieve body (202) and a sieve mesh (203); one end of the hot melt tube (201) is detachably connected to the output end of the sieve body (202), and the other end is connected to the feed port of the storage chamber (101); the end of the sieve body (202) away from the hot melt tube (201) is detachably connected to the sieve mesh (203) via a groove.

3. The cell freezing bag with cell screening function according to claim 1, characterized in that: The anti-blocking mechanism further comprises a blocking rubber pad (302), and the blocking rubber pad (302) is fixedly connected to the end surface of the telescopic tube (301) away from the material storage chamber (101).

4. The cell freezing bag with cell screening function according to claim 1, characterized in that: The anti-blocking mechanism further comprises a protective cover (303), which is detachably connected to the end of the telescopic tube (301) and is used to cover the blocking rubber pad (302).

5. The cell freezing bag with cell screening function according to claim 1, characterized in that: The bag body (10) comprises a containing bag (102) and a protective bag (103); the storage cavity (101) is opened in the containing bag (102); a buffer cavity is opened inside the protective bag (103); and the containing bag (102) is arranged in the buffer cavity.

6. The cell freezing bag with cell screening function according to claim 1, characterized in that: The bag body (10) further comprises a cotton block (104), wherein the cotton block (104) is fixedly connected to the inner wall of the buffer cavity, the cotton block (104) abuts against the receiving bag (102), and the cotton block (104) is used to space the receiving bag (102) from the inner wall of the buffer cavity.

Images