Mobile phase classified collection device for high performance liquid chromatography

Abstract

The utility model relates to a mobile phase classifying and collecting device for high performance liquid chromatography, which comprises a high performance liquid chromatograph and a mobile phase collecting tank, and the input end of the mobile phase collecting tank is connected with the output end of the high performance liquid chromatograph; the information acquisition module is arranged between the output end of the high performance liquid chromatograph and the input end of the mobile phase collection tank, and the information acquisition module is used for being linked with a high performance liquid chromatography detection method in real time and acquiring the real-time information of a mobile phase; the controller is electrically connected with the output end of the information acquisition module and receives the mobile phase real-time information acquired by the information acquisition module; the liquid inlet valve is arranged between the output end of the information acquisition module and the input end of the mobile phase collection tank, the liquid inlet valve is connected with the output end of the controller, and the controller controls opening and closing of the liquid inlet valve according to the received mobile phase real-time information. The device is simple in structure and practices the concepts of conservation and environmental protection.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatography, in particular to a mobile phase classification and collection device for high performance liquid chromatography. Background Art

[0002] During the research and development and testing process in the laboratory, high performance liquid chromatography and ultra-high performance liquid chromatography are used frequently, especially in the testing laboratories of production units. The liquid phase works almost non-stop, so a lot of waste liquid is generated by the mobile phase. The mobile phase contains a large amount of methanol, ethanol, acetonitrile, acid, alkali and other components, which cannot be discarded directly. Professional companies must be invited to recycle the waste liquid on site. There are safety hazards during the storage of the waste liquid in the laboratory, and the environmental pollution caused by the treatment of the waste liquid is also relatively large. Utility Model Content

[0003] The utility model aims to provide a mobile phase classification and collection device for high performance liquid chromatography, which solves the problem in the prior art that the mobile phase of high performance liquid chromatography cannot be reasonably disposed of after use.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0005] The utility model provides a mobile phase classification and collection device for high performance liquid chromatography, comprising:

[0006] High performance liquid chromatography,

[0007] A mobile phase collection tank, wherein the input end of the mobile phase collection tank is connected to the output end of the high performance liquid chromatograph;

[0008] An information acquisition module is provided between the output end of the high performance liquid chromatograph and the input end of the mobile phase collection tank, and is used to work in real time with the high performance liquid chromatography detection method to collect real-time information of the mobile phase;

[0009] A controller, the controller being electrically connected to the output end of the information acquisition module and receiving the real-time information of the mobile phase acquired by the information acquisition module;

[0010] The liquid inlet valve is arranged between the output end of the information acquisition module and the input end of the mobile phase collection tank, and the liquid inlet valve is connected to the output end of the controller. The controller controls the switch of the liquid inlet valve according to the received real-time information of the mobile phase.

[0011] Furthermore, the mobile phase collection tank includes a single-component mobile phase collection tank, a multi-component mobile phase collection tank and a post-shutoff mobile phase collection tank. The single-component mobile phase collection tank is used to collect the single-component system mobile phase and recycle it, the multi-component mobile phase collection tank is used to collect the multi-component system mobile phase and treat it as waste liquid, and the post-shutoff mobile phase collection tank is used to collect the mobile phase remaining in the pipeline after the liquid inlet valve is shut off.

[0012] Furthermore, the single-component mobile phase collection tank includes an ethanol mobile phase collection tank, a methanol mobile phase collection tank and an acetonitrile mobile phase collection tank.

[0013] Furthermore, the single-component mobile phase collecting tank and the multi-component mobile phase collecting tank are further provided with liquid level detectors for detecting the liquid levels in the single-component mobile phase collecting tank and the multi-component mobile phase collecting tank.

[0014] Furthermore, a flow meter is provided between the information acquisition module and the liquid inlet valve, and the flow meter is used to monitor the real-time flow of the mobile phase.

[0015] Due to the application of the above technical solution, the utility model has the following advantages compared with the prior art:

[0016] The utility model discloses a mobile phase classification and collection device for high performance liquid chromatography, which collects the mobile phase used in the current work through an information collection module linked to a high performance liquid chromatography detection method, and opens a corresponding liquid inlet valve through a controller to allow the mobile phase to flow into a corresponding mobile phase collection tank for subsequent recycling and treatment, thereby avoiding the problem in the prior art that the mobile phase cannot be disposed of in time after use, thereby affecting the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Hereinafter, some specific embodiments of the present invention will be described in detail in an exemplary and non-limiting manner with reference to the accompanying drawings. The same reference numerals in the accompanying drawings indicate the same or similar components or parts. It should be understood by those skilled in the art that these drawings are not necessarily drawn to scale. In the accompanying drawings:

[0018] Figure 1 The utility model provides a mobile phase classification and collection device for high performance liquid chromatography;

[0019] The description of the accompanying drawings is as follows:

[0020] 1. High-performance liquid chromatograph; 2. Mobile phase collection tank; 201. Single-component mobile phase collection tank; 2010. Ethanol mobile phase collection tank; 2011. Methanol mobile phase collection tank; 2012. Acetonitrile mobile phase collection tank; 202. Multi-component mobile phase collection tank; 203. Shut-off mobile phase collection tank; 3. Information acquisition module; 4. Controller; 5. Liquid inlet valve; 6. Liquid level detector; 7. Flow meter. DETAILED DESCRIPTION

[0021] The following is a clear and complete description of the technical solution of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0022] See Figure 1 The mobile phase classification and collection device for high performance liquid chromatography comprises a high performance liquid chromatograph 1, a mobile phase collection tank 2, an information acquisition module 3, a controller 4 and a liquid inlet valve 5. The arrows shown in the figure indicate the direction of signal transmission involved in the device.

[0023] Specifically, the mobile phase collection tank 2 is disposed downstream of the HPLC 1, i.e., the output of the HPLC 1 is connected to the input of the mobile phase collection tank 2. More specifically, the HPLC 1 and the mobile phase collection tank 2 are connected via corresponding pipes, and the mobile phase eluted by the HPLC 1 is transported to the mobile phase collection tank 2 to complete the collection of the mobile phase. At the same time, whether the collected mobile phase is recycled is determined based on the properties and components of the collected mobile phase.

[0024] In the embodiment of the present utility model, the aforementioned mobile phase collecting tank 2 is provided with several, including a single-component mobile phase collecting tank 201, a multi-component mobile phase collecting tank 202 and a mobile phase collecting tank 203 after being shut off, and the single-component mobile phase collecting tank 201 is used to collect the single-component mobile phase. Since there is only a kind of organic phase in the single-component mobile phase, the single-component mobile phase can be recycled. Specifically, the collected single-component mobile phase can be distilled or evaporated and recovered by a rotary evaporator, and the mobile phase obtained after purification is used. The collected single-component mobile phase can also be directly used as a lotion to clean the reagent bottle, and with the help of the principle of like dissolves like, some insoluble impurities or stains in the reagent bottle are removed. Of course, it is not limited to the mobile phase recycling method described above, as long as the single-component mobile phase can be reasonably recycled, it is possible to avoid reagent waste and pollute the environment.

[0025] In the embodiment of the present invention, the above-mentioned single-component mobile phase collection tank 201 includes an ethanol mobile phase collection tank 2010, a methanol mobile phase collection tank 2011 and an acetonitrile mobile phase collection tank 2012. Of course, it is not limited to the collection tanks of the above-mentioned reagents. For example, a collection tank can be set when acetone is used as the mobile phase. As long as the reagent can meet the requirements of a single component and can play a good elution role on the sample to be tested, a corresponding collection tank can be arranged.

[0026] The multi-component mobile phase collecting tank 202 is used to collect a mixed mobile phase of multiple components, including two or more organic phases. Since it is a mixed mobile phase of multiple components, the mobile phase should be treated as waste liquid.

[0027] The information collection module 3 is provided between the output end of the HPLC 1 and the input end of the mobile phase collection tank 2, and is used to link with the HPLC detection method in real time to collect information about the mobile phase used in the current detection method.

[0028] Regarding how the information acquisition module 3 is linked to the high-performance liquid chromatography method, data exchange between the information acquisition module and the liquid chromatograph can be achieved through standard communication protocols such as USB, RS232, and Ethernet. Alternatively, the collected mobile phase information can be formatted for compatibility with the liquid chromatography detection system, and then data analysis algorithms and pattern recognition techniques can be used to analyze changes in the mobile phase. As long as the mobile phase information used in the current operating mode can be accurately collected.

[0029] At the same time, in the embodiment of the present invention, a controller 4 is further provided. The controller 4 is electrically connected to the output end of the aforementioned information acquisition module 3 and is used to receive the practical information about the mobile phase collected by the information acquisition module 3 .

[0030] Regarding the above-mentioned liquid inlet valve 5, in the embodiment of the present invention, it is set between the output end of the aforementioned information acquisition module 3 and the input end of the mobile phase collection tank 2. In addition, the liquid inlet valve 5 is connected to the output end of the aforementioned controller 4. When the controller 4 receives the mobile phase information transmitted by the information acquisition module 3, the controller 4 will open the liquid inlet valve 5 of the corresponding mobile phase tank according to the information, thereby introducing the mobile phase into the corresponding mobile phase tank. The opening and closing of the liquid inlet valve 5 is controlled by the controller 4. A liquid inlet valve 5 is set between each mobile phase collection tank 2 and the information acquisition module 3, so that one channel and one valve are switched on and off.

[0031] In addition, in the embodiment of the present invention, a liquid level meter 6 is provided in each of the single-component mobile phase collection tank 201 and the multi-component mobile phase collection tank 202 to detect the liquid level in the corresponding mobile phase tank, thereby preventing the mobile phase from overflowing the tank and causing environmental pollution. When the liquid level detected by the liquid level meter 6 exceeds the maximum capacity of the mobile phase tank, the liquid level meter 6 sends a signal to the controller 4, which, upon receiving the signal, sends a shutoff command to the liquid inlet valve 5.

[0032] In addition, after the liquid inlet valve 5 receives the shutoff command, some mobile phase will still remain in the pipeline. If this part of the mobile phase is not discharged in time, it will affect the collection of the next mobile phase, causing the rear end residue of the previously collected mobile phase to mix with the front end liquid of the later collected mobile phase, and then flow into the later collected mobile phase collection tank, affecting the purity of the mobile phase in the single component collection tank. In the embodiment of the present utility model, an additional post-shutoff mobile phase collection tank 203 is provided to collect the mobile phase remaining in the pipeline after the liquid inlet valve 5 is shut off, to prevent it from mixing with the subsequently collected mobile phase and affecting the collection of the single component mobile phase.

[0033] Furthermore, after collecting the mobile phase in the current working state and issuing a closing command to the corresponding inlet valve 5, the controller 4 will issue an opening command to the inlet valve 5 corresponding to the mobile phase collection tank 203. When the HPLC 1 starts the next phase of detection, the controller 4 will issue a closing command to the inlet valve 5 corresponding to the mobile phase collection tank 203.

[0034] A flow meter 7 is further provided between the information acquisition module 3 and the liquid inlet valve 5 for monitoring the real-time flow of the mobile phase.

[0035] In summary, the utility model is a mobile phase classification and collection device for high performance liquid chromatography, which integrates a mobile phase collection tank, a mobile phase information acquisition module, a controller, and a liquid inlet valve corresponding to each mobile phase collection tank at the back end of a high performance liquid chromatograph. The liquid phase detection method under the working state of the above-mentioned information acquisition module is linked, and the information of the mobile phase used under the working state is transmitted to the controller. After receiving the signal, the controller will send an opening instruction to the liquid inlet valve at the collection tank corresponding to the flow, so as to introduce the mobile phase into the corresponding collection tank. In addition, a liquid level detector is provided in each collection tank to monitor the mobile phase liquid level in the tank body in real time, thereby preventing the mobile phase from overflowing and polluting the environment. A flow meter is also provided synchronously to monitor the flow rate of the mobile phase in real time to ensure the circulation of the mobile phase. The device has a simple structure and is economical and environmentally friendly.

[0036] The above embodiments are only for illustrating the technical concept and features of the present invention. Their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it accordingly. They are not intended to limit the scope of protection of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of protection of the present invention.

Claims

1. A mobile phase classification and collection device for high performance liquid chromatography, characterized in that: include: High performance liquid chromatography (1), A mobile phase collection tank (2), wherein the input end of the mobile phase collection tank (2) is connected to the output end of the high performance liquid chromatograph (1); An information acquisition module (3), the information acquisition module (3) being arranged between the output end of the high performance liquid chromatograph (1) and the input end of the mobile phase collection tank (2), the information acquisition module (3) being used to be linked in real time with the high performance liquid chromatography detection method to collect real-time information of the mobile phase; A controller (4), the controller (4) being electrically connected to the output end of the information acquisition module (3) and receiving the real-time information of the mobile phase acquired by the information acquisition module (3); A liquid inlet valve (5) is provided between the output end of the information acquisition module (3) and the input end of the mobile phase collection tank (2), and the liquid inlet valve (5) is connected to the output end of the controller (4). The controller (4) controls the opening and closing of the liquid inlet valve (5) according to the received real-time information of the mobile phase.

2. A mobile phase classification and collection device for high performance liquid chromatography according to claim 1, characterized in that: The mobile phase collection tank (2) comprises a single-component mobile phase collection tank (201), a multi-component mobile phase collection tank (202) and a shut-off mobile phase collection tank (203). The single-component mobile phase collection tank (201) is used to collect the single-component system mobile phase and recycle it. The multi-component mobile phase collection tank (202) is used to collect the multi-component system mobile phase and treat it as waste liquid. The shut-off mobile phase collection tank (203) is used to collect the mobile phase remaining in the pipeline after the liquid inlet valve (5) is shut off.

3. A mobile phase classification and collection device for high performance liquid chromatography according to claim 2, characterized in that, The single-component mobile phase collection tank (201) comprises an ethanol mobile phase collection tank (2010), a methanol mobile phase collection tank (2011), and an acetonitrile mobile phase collection tank (2012).

4. A mobile phase classification and collection device for high performance liquid chromatography according to claim 2, characterized in that: The single-component mobile phase collection tank (201) and the multi-component mobile phase collection tank (202) are further provided with a liquid level detector (6) for detecting the liquid level in the single-component mobile phase collection tank (201) and the multi-component mobile phase collection tank (202).

5. The mobile phase classification and collection device for high performance liquid chromatography according to claim 1, characterized in that: A flow meter (7) is further provided between the information acquisition module (3) and the liquid inlet valve (5), and the flow meter is used to monitor the real-time flow rate of the mobile phase.

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