Fluorescence detection device and sample detection equipment
By adopting the design of two excitation light paths sharing one emission light path in the fluorescence detection device, the existing PCR instrument fluorescence detection device has solved the problem of large size and high cost, and a compact and practical fluorescence detection is achieved, supporting multiple PCR and portable applications with high sensitivity.
Patent Information
- Application Number
- CN202421525316.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-28
- Publication Date
- 2025-08-22
- Estimated Expiration
- 2034-06-28
AI Technical Summary
The existing PCR instrument fluorescence detection device is large in size, high in cost and is not suitable for portable POCT application scenarios. The fluorescent emitted light in the existing coaxial optical path configuration is easily affected by excitation light and has low sensitivity.
The design of two excitation light paths sharing one emission light path is adopted. The excitation light path and the emission light path are arranged 90°. The excitation light path is located at the bottom or side of the reaction vessel. The excitation light source emits different colors of light into the reaction liquid through the excitation filter and the excitation focus mirror. The fluorescence is detected by the detector through the emission collimator, the emission filter and the emission focus mirror, and avoid the use of dichroic mirrors.
It realizes a compact and practical fluorescence detection device, supports fluorescence detection in at least 4 channels, reduces costs and improves detection reliability and sensitivity, and is suitable for portable POCT applications.
Smart Images
Figure CN223259564U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of sample detection, and in particular relates to a fluorescence detection device and sample detection equipment. Background Art
[0002] PCR is a molecular biology technique that amplifies specific DNA fragments in vitro. Its greatest strength is its ability to significantly increase the amount of DNA present in minute quantities. PCR is widely used in molecular biology testing and analysis due to its high specificity, high sensitivity, minimal purity requirements, simplicity, and rapidity.
[0003] Currently, mainstream PCR instrument fluorescence detection systems utilize rotating disk scanning and fiber optic light guidance. These fluorescence detection systems are bulky, inconvenient to assemble, and expensive. Other devices utilize camera-based fluorescence detection, which is costly and bulky, and requires sealing the consumables before use. Neither optical path is suitable for portable point-of-care (POCT) applications.
[0004] Some portable devices on the market use a coaxial optical path configuration, where the fluorescence excitation light path and the fluorescence emission light path are coaxially arranged in a mechanical housing, and a dichroic mirror is used to separate the laser light and the emission light. This configuration makes the fluorescence emission light easily affected by the excitation light, and in practice, the fluorescence background is high, resulting in low system sensitivity. Utility Model Content
[0005] The main purpose of the utility model is to provide a fluorescence detection device and a sample detection equipment, aiming to solve the technical problems of large size and high cost of the fluorescence detection device of the sample detection equipment in the prior art.
[0006] In order to achieve the above-mentioned purpose, the utility model provides a fluorescence detection device, which includes: a consumables holder, which is provided with a sample hole, two light entrance holes and a light exit hole, the sample hole is used to accommodate a reaction container, the light exit hole is arranged at the bottom of the sample hole, and the two light entrance holes are arranged on the side of the sample hole along the radial direction of the sample hole; two excitation light paths and the two light entrance holes are arranged in one-to-one correspondence, and an excitation light source, an excitation filter and an excitation focusing mirror are sequentially arranged on the excitation light path; an emission light path is arranged corresponding to the light exit hole, and the two excitation light paths share one emission light path, and an emission collimator, an emission filter, an emission focusing mirror and a detector are sequentially arranged on the emission light path, and the light emitted by the excitation light source can pass through the excitation light path, the reaction container and be transmitted to the detector in sequence.
[0007] In an embodiment of the present utility model, the fluorescence detection device includes an excitation lens barrel and an emission lens barrel, the excitation lens barrel is plugged into the light entrance hole, the emission lens barrel is plugged into the light exit hole, the excitation light source, the excitation filter and the excitation focusing mirror are arranged in the excitation lens barrel, and the emission collimator, the emission filter, the emission focusing mirror and the detector are arranged in the emission lens barrel.
[0008] In an embodiment of the present utility model, the excitation lens barrel is opened at both ends along the axial direction, one end of the excitation lens barrel is inserted into the light entrance hole, the light source circuit board cover of the laser source is arranged at the other end of the excitation lens barrel, and a wire hole is provided on the light source circuit board; and / or, a first step groove for clamping the excitation filter and a second step groove for clamping the excitation focusing mirror are provided in the excitation lens barrel.
[0009] In an embodiment of the present utility model, a third step groove for clamping the emission collimating lens, a fourth step groove for clamping the emission filter, and a fifth step groove for clamping the emission focusing lens are provided in the emission lens barrel.
[0010] In an embodiment of the present invention, the fluorescence detection device further comprises an inner sleeve clamped to the fifth stepped groove, and the inner sleeve is provided with a clamping slot for clamping the emission focusing mirror.
[0011] In an embodiment of the present invention, the two ends of the emitting lens barrel are opened in the axial direction, one end of the emitting lens barrel is plugged into the light exit hole, and the driving circuit board sealing cover of the detector is arranged at the other end of the emitting lens barrel.
[0012] In an embodiment of the present invention, the excitation light path further includes an excitation collimator located between the excitation light source and the excitation filter; and / or, one of the excitation light sources can emit light of at least two different colors, and the excitation filter is a multi-bandpass filter.
[0013] In an embodiment of the present invention, the excitation light path includes at least two excitation light sources, at least two of the excitation light sources can emit light of different colors, the excitation filter is a single-bandpass filter, the number of the excitation filters and the excitation light sources is the same and they are arranged in a one-to-one correspondence, or the excitation filter is a multi-bandpass filter.
[0014] In an embodiment of the present invention, the emission filter is a single-bandpass filter or a multi-bandpass filter; and / or the detector is a photodiode single-point detector, an array detector, a four-quadrant detector, a CMOS or CCD imaging device.
[0015] The present invention also provides a sample detection device, which includes the fluorescence detection device described above.
[0016] Through the above technical solution, the fluorescence detection device provided by the embodiment of the utility model has the following beneficial effects:
[0017] When a fluorescence detection device is used for fluorescence detection, two excitation light paths share one emission light path. The emission light path is located at the bottom or side of the reaction vessel and is arranged at 90° to the excitation light path. When each excitation light path can emit at least two colors of light, fluorescence emission detection of at least four fluorescent probes can be achieved. The excitation light path and the emission light path can also be swapped, without any moving parts, with a simple and compact structure. The excitation light path is located near the bottom side of the reaction vessel, and a single side supports the excitation of at least two channels. The light path is flattened. The light emitted by this excitation light source can pass through the excitation filter, the excitation focusing mirror, and the light input hole into the reaction liquid in the reaction vessel for fluorescence excitation. The fluorescence can pass through the light output hole into the emission collimator, the emission filter, the emission focusing mirror, and the detector, and fluorescence detection can be performed through the detector. The two excitation light paths in the utility model share one emission light path, and the excitation and emission only occupy one bottom surface and two side surfaces of the consumables holder, making the fluorescence detection device small, compact and practical; the remaining side surface of the consumables holder can be used for the installation of the temperature control device, there are no moving or dynamic parts in the light path, and fluorescence detection can be achieved without the use of a dichroic mirror, with high reliability. When the excitation light source can emit light of different colors, at least 4 channels of fluorescence detection can be achieved; multiple PCR is supported.
[0018] Other features and advantages of the present invention will be described in detail in the subsequent detailed description of the embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] The accompanying drawings are used to provide an understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention but do not constitute a limitation of the present invention. In the accompanying drawings:
[0020] Figure 1 It is a structural schematic diagram of a fluorescence detection device according to one embodiment of the present utility model;
[0021] Figure 2 is a schematic cross-sectional structural diagram of a fluorescence detection device according to one embodiment of the present utility model;
[0022] Figure 3 This is a schematic diagram of the structure of an excitation lens barrel of a fluorescence detection device according to one embodiment of the present utility model;
[0023] Figure 4 It is a schematic diagram of the structure of the emission lens barrel of the fluorescence detection device according to one embodiment of the present utility model.
[0024] Description of Reference Numerals
[0025] Label Name Label Name
[0026] 100 Fluorescence detection device 34 Detector
[0027] 1 Consumables holder 4 Excitation tube
[0028] 11 Sample hole 41 First step groove
[0029] 12 Light entry hole 42 Second step groove
[0030] 13 light exit hole 5 emission tube
[0031] 2 Excitation light path 51 Third step groove
[0032] 21 Excitation light source 52 Fourth step groove
[0033] 22 Excitation filter 53 Fifth step groove
[0034] 23 Excitation focusing mirror 6 inner sleeve
[0035] 3 Transmitting optical path 61 slot
[0036] 31 Emitting collimator 7 Light source circuit board
[0037] 32 Emission filter 8 Driver circuit board
[0038] 33 Launch focusing mirror DETAILED DESCRIPTION
[0039] The following is a detailed description of the specific embodiments of the present invention in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention and are not intended to limit the present invention.
[0040] The fluorescence detection device according to the present invention will be described below with reference to the accompanying drawings.
[0041] like Figures 1 to 4As shown, the fluorescence detection device 100 includes a consumable holder 1, an emission light path 3 and two excitation light paths 2. The consumable holder 1 is provided with a sample hole 11, two light entrance holes 12 and a light exit hole 13. The sample hole 11 is used to accommodate a reaction vessel. The light exit hole 13 is arranged at the bottom of the sample hole 11, and the two light entrance holes 12 are arranged on the side of the sample hole 11 along the radial direction of the sample hole 11; the two excitation light paths 2 and the two light entrance holes 12 are arranged in a one-to-one correspondence, and the excitation light path 2 is sequentially provided with an excitation light source 21, an excitation filter 22 and an excitation focusing mirror 23; the emission light path 3 is arranged corresponding to the light exit hole 13, and the two excitation light paths 2 share one emission light path 3, and the emission light path 3 is sequentially provided with an emission collimator 31, an emission filter 32, an emission focusing mirror 33 and a detector 34. The light emitted by the excitation light source 21 can pass through the excitation light path 2 and the reaction vessel in sequence and be transmitted to the detector 34.
[0042] like Figures 2 to 4 As shown, the light entrance hole 12 and the light exit hole 13 in this embodiment are both conical, and the conical bottom surfaces of the light entrance hole 12 and the light exit hole 13 are arranged away from the sample hole 11, and the tips of the light entrance hole 12 and the light exit hole 13 are arranged close to the sample hole 11. The top of the sample hole 11 is open, and the reaction container can be placed into the sample hole 11 through the top. The central axes of the light entrance hole 12 and the light exit hole 13 are arranged perpendicular to each other, so that the fluorescence detection device 100 in this embodiment has a compact structure. The excitation light source 21 can be an LED lamp, and an LED with a low divergence angle can be used. In one embodiment, the excitation light source 21 is an LED with a divergence angle less than 15 degrees. Figure 1 As shown, the number of excitation light sources 21 in this embodiment is four, and each light entrance hole 12 corresponds to an excitation light source 21. In other embodiments, the number of excitation light sources 21 is set according to actual usage requirements.
[0043] When the fluorescence detection device 100 in this embodiment is used for fluorescence detection, two excitation light paths 2 share one emission light path 3. The emission light path 3 is located at the bottom or side of the reaction vessel and is arranged at 90° to the excitation light path 2. When each excitation light path 2 can emit at least two colors of light, fluorescence emission detection of at least four fluorescent probes can be achieved. The excitation light path 2 and the emission light path 3 can also be swapped in position. There are no moving parts, and the structure is simple and compact. The excitation light path 2 is located near the bottom side of the reaction vessel, and a single side supports the excitation of at least two channels. The light path is flattened. The light emitted by the excitation light source 21 can enter the reaction liquid in the reaction vessel through the excitation filter 22, the excitation focusing lens 23, and the light input hole 12 to perform fluorescence excitation. The fluorescence can enter the emission collimator 31, the emission filter 32, the emission focusing lens 33 and the detector 34 through the light output hole 13, and fluorescence detection can be performed through the detector 34. In this embodiment, the two excitation light paths 2 share one emission light path 3, and the excitation and emission only occupy one bottom surface and two side surfaces of the consumable holder 1, making the fluorescence detection device 100 small, compact and practical; the remaining side surface of the consumable holder 1 can be used for the installation of the temperature control device. There are no moving or dynamic parts in the light path, and fluorescence detection can be achieved without using a dichroic mirror. While saving costs, the reliability of the fluorescence detection device 100 can also be increased. When the excitation light source 21 can emit light of different colors, at least 4 channels of fluorescence detection can be achieved; multiple PCR is supported.
[0044] like Figure 1 and Figure 2 As shown, the fluorescence detection device 100 includes an excitation lens barrel 4 and an emission lens barrel 5 that are perpendicular to each other. The excitation lens barrel 4 is plugged into the light entrance hole 12, and the emission lens barrel 5 is plugged into the light exit hole 13. The excitation light source 21, the excitation filter 22 and the excitation focusing lens 23 are arranged in the excitation lens barrel 4, and the emission collimator 31, the emission filter 32, the emission focusing lens 33 and the detector 34 are arranged in the emission lens barrel 5. The excitation lens barrel 4 and the emission lens barrel 5 in this embodiment are both hollow cylindrical with openings at both ends, with a simple structure and easy production. The ends of the excitation lens barrel 4 and the emission lens barrel 5 are both provided with plug-in protrusions for plugging into the consumable holder 1. Through the cooperation of the concave and convex structures, the assembly of the excitation lens barrel 4, the emission lens barrel 5 and the consumable holder 1 can be completed. In other embodiments, the angle between the excitation lens barrel 4 and the emission lens barrel 5 can be set according to actual use requirements.
[0045] In one embodiment, the fluorescence detection device 100 may include a consumables holder 1, two reaction containers, four excitation lens barrels 4, and two emission lens barrels 5; the four-color fluorescence detection of each reaction container is completed by two excitation lens barrels 4 and one emission lens barrel 5; the excitation lens barrel 4 is equipped with an excitation light source 21, an excitation collimator, an excitation filter 22 and an excitation focusing lens 23; the optical path configuration inside the excitation lens barrel 4 can be adjusted according to cost, implementation difficulty, fluorescence channel crosstalk and sensitivity; the excitation light source 21 can be in the form of a two-color LED or a combination of two monochromatic LEDs; the excitation filter 22 can be a dual-bandpass filter or a single-bandpass filter combination; the emission lens barrel 5 is equipped with an emission collimator 31, an emission filter 32, an emission focusing lens 33 and a detector 34, and the optical path configuration inside the excitation lens barrel 4 can be adjusted according to cost, implementation difficulty, fluorescence channel crosstalk and sensitivity; for example, the emission filter 32 can be a four-bandpass filter, or a combination of two dual-bandpass filters. The light emitted by the excitation light source 21 is collimated by the excitation collimator, filtered by the excitation filter 22, focused by the excitation focusing lens 23, passes through the light entrance hole 12, and through the transparent side wall of the reaction vessel, and is incident on the reaction liquid in the reaction vessel; excites the reaction liquid inside the reaction vessel; emits fluorescence; the emission light path 3 is installed below the bottom of the reaction vessel; and can receive the fluorescence emitted by the reaction vessel.
[0046] In one embodiment, the excitation lens barrel 4 is opened at both ends along the axial direction, one end of the excitation lens barrel 4 is inserted into the light entrance hole 12, the light source circuit board 7 of the excitation light source 21 is covered on the other end of the excitation lens barrel 4, and a wire hole is provided on the light source circuit board 7; and a first step groove 41 for clamping the excitation filter 22 and a second step groove 42 for clamping the excitation focusing lens 23 are provided in the excitation lens barrel 4.
[0047] The excitation light source 21 in this embodiment is mounted on the light source circuit board 7, and connecting wires can be connected to the excitation light source 21 through the wire holes. In this embodiment, the excitation lens barrel 4 is blocked by the light source circuit board 7, which not only ensures a seal but also reduces the space required for the installation of the light source circuit board 7, further reducing the size of the fluorescence detection device 100. The first stepped groove 41 and the second stepped groove 42 correspond to the corresponding clamping of the excitation filter 22 and the excitation focusing lens 23, respectively, which can facilitate the assembly of the excitation optical path 2.
[0048] like Figure 4 As shown, the emission barrel 5 is provided with a third step groove 51 for snapping in the emission collimator 31, a fourth step groove 52 for snapping in the emission filter 32, and a fifth step groove 53 for snapping in the emission focusing lens 33. In this embodiment, the emission collimator 31, the emission filter 32, and the emission focusing lens 33 are all connected to the emission barrel 5 by snapping in, which can facilitate the installation and positioning of the emission optical path 3.
[0049] In one embodiment, the fluorescence detection device 100 further includes an inner sleeve 6 that is engaged with the fifth stepped groove 53. The inner sleeve 6 defines a slot 61 for engaging the emission focusing lens 33. In this embodiment, the inner sleeve 6 is a hollow cylindrical structure. The inner sleeve 6 and the emission lens barrel 5 cooperate to form a double-layer sleeve structure, which facilitates the positioning and installation of the emission focusing lens 33.
[0050] Specifically, both ends of the emitting lens barrel 5 along the axial direction are open, one end of the emitting lens barrel 5 is plugged into the light exit hole 13, and the driving circuit board 8 of the detector 34 is sealed and covered at the other end of the emitting lens barrel 5. In this embodiment, the driving circuit board 8 seals the end of the emitting lens barrel 5 away from the consumables holder 1, and the detector 34 is mounted on the driving circuit board 8, which can solve the installation space in the emitting lens barrel 5 and improve the compactness of the structure in the emitting lens barrel 5.
[0051] In one embodiment, the excitation optical path 2 further includes an excitation collimator positioned between the excitation light source 21 and the excitation filter 22. Light emitted by the excitation light source 21 is collimated by the excitation collimator before passing through the excitation filter 22, ensuring accurate light transmission. Furthermore, in one embodiment, a single excitation light source 21 can emit light of at least two different colors, and the excitation filter 22 is a multi-bandpass filter.
[0052] Specifically, an excitation light source 21 is provided in each excitation light path 2, and one excitation light source 21 can emit light of two different colors, so that one excitation light path 2 can realize the detection of two fluorescence channels, and the passband of the single-piece excitation filter 22 is set corresponding to the excitation light source 21.
[0053] In another embodiment, the excitation light path 2 includes at least two excitation light sources 21, and the at least two excitation light sources 21 can emit light of different colors. The excitation filter 22 is a single-bandpass filter. The number of excitation filters 22 and the excitation light sources 21 are the same and are arranged one-to-one. The combination of multiple single-bandpass filters can reduce the production cost of the excitation light path 2; or the excitation filter 22 is a multi-bandpass filter. A multi-bandpass filter can reduce the setting of multiple filtering components and simplify the assembly process.
[0054] In an embodiment of the present invention, the emission filter 32 is a single-bandpass filter or a multi-bandpass filter; and the detector 34 is a photodiode single-point detector, an array detector, a four-quadrant detector, a CMOS or a CCD imaging device.
[0055] In one embodiment, a fluorescence detection device 100 includes a consumables holder 1, two reaction vessels, four excitation lens barrels 4, and two emission lens barrels 5. Each pair of excitation lens barrels 4 excites a single reaction vessel, while a single emission lens barrel receives fluorescence. Each excitation lens barrel 4 is internally mounted with a dual-color LED, a dual-bandpass excitation filter 22, and an excitation focusing lens 23. The two excitation lens barrels 4 enable four-color excitation. Each emission lens barrel 5 is internally mounted with an emission collimator 31, a four-bandpass emission filter 32, an inner sleeve 6, an emission focusing lens 33, and a detector 34. The emission filter 32 can be composed of an array of four single-bandpass filters or a single four-bandpass filter. If the emission filter 32 is a four-bandpass filter, each filter corresponds to a detection channel. If the emission filter 32 is a single four-bandpass filter, each bandpass corresponds to a detection channel, and the filter cutoff depth is greater than OD5.
[0056] In other embodiments, two upper and lower LEDs can be used in each excitation light path 2 instead of a two-color LED; the upper filter and the lower filter are both single-bandpass filters; when the light emitted by the upper and lower LEDs is close to parallel light, such as when the divergence angle is less than 3°; the array lens can be removed; the peak wavelength of the upper LED corresponds one-to-one to the center wavelength of the upper bandpass filter; the peak wavelength of the lower LED corresponds one-to-one to the center wavelength of the lower bandpass filter.
[0057] In one embodiment, each excitation optical path 2 may use an upper and lower LED instead of a dual-color LED; the filter still uses a dual-bandpass filter; when the light emitted by the upper and lower LEDs is close to parallel light, such as when the divergence angle is less than 3°, the array lens can be removed; the peak wavelengths of the upper and lower LEDs correspond one-to-one to the two passband center wavelengths of the dual-bandpass filter;
[0058] In another embodiment, there are two emission filters 32 in the emission light path 3, and both emission filters 32 are dual-bandpass filters instead of four-bandpass filters; two detectors 34, one of which receives fluorescence passing through one of the emission filters 32; and the other detector 34 receives fluorescence passing through the other emission filter 32.
[0059] The present invention also provides a sample detection device, which includes the fluorescence detection device 100 described above. The sample detection device may include a temperature control device in the prior art, which can adjust the temperature of the consumable holder 1. The sample detection device includes at least two fluorescence detection devices 100, with the consumable holder 1 side walls of two adjacent fluorescence detection devices 100 connected, which can support multi-tube detection and at least four channels of fluorescence detection simultaneously. This significantly increases the detection throughput, and the device is compact and low-cost, making it suitable for use in POCT fluorescence detection devices. In one embodiment, the sample detection device is a nucleic acid amplification device.
[0060] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of this utility model, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.
[0061] In this utility model, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection, or communication; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. For those skilled in the art, the specific meanings of the above terms in this utility model can be understood according to specific circumstances.
[0062] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.
[0063] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are illustrative and cannot be understood as limitations on the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention.
Claims
1. A fluorescence detection device, characterized in that: The fluorescence detection device (100) comprises: A consumables holder (1) is provided with a sample hole (11), two light entrance holes (12) and a light exit hole (13), wherein the sample hole (11) is used to accommodate a reaction container, the light exit hole (13) is arranged at the bottom of the sample hole (11), and the two light entrance holes (12) are arranged on the side of the sample hole (11) along the radial direction of the sample hole (11); Two excitation light paths (2) are provided in one-to-one correspondence with the two light entrance holes (12), and an excitation light source (21), an excitation filter (22), and an excitation focusing mirror (23) are sequentially provided on the excitation light path (2); An emission light path (3) is provided corresponding to the light exit hole (13); the two excitation light paths (2) share one emission light path (3); an emission collimator (31), an emission filter (32), an emission focusing lens (33) and a detector (34) are sequentially provided on the emission light path (3); and light emitted by the excitation light source (21) can sequentially pass through the excitation light path (2), the reaction container and be transmitted to the detector (34).
2. The fluorescence detection device according to claim 1, characterized in that The fluorescence detection device (100) comprises an excitation lens barrel (4) and an emission lens barrel (5), wherein the excitation lens barrel (4) is plugged into the light entrance hole (12), and the emission lens barrel (5) is plugged into the light exit hole (13); the excitation light source (21), the excitation filter (22) and the excitation focusing lens (23) are arranged in the excitation lens barrel (4); and the emission collimator (31), the emission filter (32), the emission focusing lens (33) and the detector (34) are arranged in the emission lens barrel (5).
3. The fluorescence detection device according to claim 2, characterized in that: The excitation lens barrel (4) is provided with openings at both ends along the axial direction, one end of the excitation lens barrel (4) is plugged into the light entrance hole (12), a light source circuit board (7) of the excitation light source is covered on the other end of the excitation lens barrel (4), and a wire hole is provided on the light source circuit board (7); and / or, A first stepped groove (41) for clamping the excitation filter (22) and a second stepped groove (42) for clamping the excitation focusing mirror (23) are provided in the excitation lens barrel (4).
4. The fluorescence detection device according to claim 2, characterized in that: The emission lens barrel (5) is provided with a third step groove (51) for clamping the emission collimating lens (31), a fourth step groove (52) for clamping the emission filter (32), and a fifth step groove (53) for clamping the emission focusing lens (33).
5. The fluorescence detection device according to claim 4, characterized in that: The fluorescence detection device (100) further comprises an inner sleeve (6) clamped to the fifth stepped groove (53), and the inner sleeve (6) is provided with a clamping slot (61) for clamping the emission focusing mirror (33).
6. The fluorescence detection device according to claim 2, characterized in that: The emitting lens barrel (5) is provided with openings at both ends along the axial direction, one end of the emitting lens barrel (5) is plugged into the light exit hole (13), and a sealing cover of a driving circuit board (8) of the detector (34) is provided at the other end of the emitting lens barrel (5).
7. The fluorescence detection device according to any one of claims 1 to 6, characterized in that: The excitation light path (2) further includes an excitation collimator located between the excitation light source (21) and the excitation filter (22); and / or, One of the excitation light sources (21) can emit light of at least two different colors, and the excitation filter (22) is a multi-bandpass filter.
8. The fluorescence detection device according to any one of claims 1 to 6, characterized in that: The excitation light path (2) comprises at least two excitation light sources (21), and the at least two excitation light sources (21) can emit light of different colors. The excitation filter (22) is a single-bandpass filter. The number of the excitation filters (22) and the excitation light sources (21) is the same and they are arranged in a one-to-one correspondence, or the excitation filter (22) is a multi-bandpass filter.
9. The fluorescence detection device according to any one of claims 1 to 6, characterized in that: The emission filter (32) is a single-bandpass filter or a multi-bandpass filter; and / or, The detector (34) is one of a photodiode single-point detector, an array detector, a four-quadrant detector, a CMOS or a CCD imaging device.
10. A sample detection device, characterized in that: The sample detection device comprises the fluorescence detection apparatus (100) according to any one of claims 1 to 9.