Embryo transplantation tube

Abstract

The utility model relates to the field of embryo transplantation, in particular to an embryo transplantation tube. Comprising a tube body, a needle head part is arranged at the far end of the tube body, the needle head part is detachably connected with the tube body, and the needle head part is a tip capable of puncturing; an injection part is arranged at the near-end position of the tube body, the injection part is detachably connected with the tube body, a cotton core part is arranged in the tube body, and the cotton core part can push liquid in the tube body to a needle head part; and the injection part is used for pushing the cotton core part to move. The clone embryo attachment efficiency and the birth rate are improved. Therefore, the efficiency of animal embryo transplantation is improved, and the method plays an important role in animal preservation and high-quality resource protection.

Description

Technical Field

[0001] The present application relates to the field of embryo transplantation, and in particular to an embryo transplantation tube. Background Art

[0002] While there are many embryo transfer devices for small and medium-sized animals, there are relatively few devices for in vitro embryo transfer. Many problems exist during embryo transfer, such as incomplete in vitro maturation of oocytes from small and medium-sized animals, which leads to low oocyte maturation rates and subsequent embryo development problems. Differences in developmental status lead to uncertainty in the transfer location. Imperfect embryo transfer devices can cause embryos to be located in different locations in the fallopian tube and uterus, affecting embryo implantation and ultimately leading to low pregnancy rates. Utility Model Content

[0003] In order to solve the problems existing in the prior art, the present application provides an embryo transfer tube.

[0004] The embryo transfer tube of the present application is mainly used for small and medium-sized animals, including but not limited to Bama pigs, cats, dogs, etc. However, when the embryo transfer tube is larger in size or in some specific use environments, it can also be used for large or medium-large animals.

[0005] The specific technical solutions of this application are as follows:

[0006] 1. An embryo transfer tube, comprising a tube body, a needle head disposed at the distal end of the tube body, the needle head being detachably connected to the tube body and having a sharp tip capable of puncture;

[0007] An injection portion is provided at the proximal end of the tube body, the injection portion being detachably connected to the tube body, and a cotton core portion being provided inside the tube body, the cotton core portion being capable of pushing the liquid inside the tube body to the needle head;

[0008] A suction device is also provided at the proximal end of the tube body, and the suction device is detachably connected to the tube body.

[0009] 2. The embryo transfer tube according to item 1 is characterized in that when the suction device sucks the embryo into the tube body at the proximal end, the embryo can enter the tube body from the distal end of the tube body, and the position of the cotton core in the tube body remains fixed.

[0010] 3. The embryo transfer tube according to item 1 is characterized in that the suction device includes a syringe and a connecting tube fixed to the syringe, the outer diameter of the connecting tube gradually decreases from the proximal end to the distal end, the connecting tube can be inserted into the tube body, and the tube body and the connecting tube are kept sealed.

[0011] 4. The embryo transfer tube according to item 1, characterized in that when the embryo is sucked into the tube body using a suction device, the suction of the embryo is stopped after the liquid containing the embryo contacts the cotton core.

[0012] 5. The embryo transfer tube according to item 1, characterized in that the injection part is used to push the cotton core part to move.

[0013] 6. The embryo transfer tube according to item 1, characterized in that the cotton core is an air cotton core, and when there are embryonic cells inside the tube body, the air cotton core can push the embryonic cells out of the tube body.

[0014] 7. The embryo transfer tube according to item 1, characterized in that the injection part includes a push rod, which is arranged inside the tube body and can move back and forth inside the tube body.

[0015] 8. The embryo transfer tube according to item 7, characterized in that the injection part further includes a fixing part, and the fixing part can be fixed to the proximal end of the tube body.

[0016] 9. The embryo transfer tube according to item 8 is characterized in that the fixing portion includes a fixing ring, which is annular; the inner diameter of the fixing ring is less than or equal to the outer diameter of the tube body, and the fixing ring is connected to the tube body by interference fit.

[0017] 10. The embryo transfer tube according to item 8, characterized in that the fixing portion includes an interference portion and a fixing ring, the fixing ring is coaxially arranged on the proximal outer side of the interference portion and is integrally formed, and the outer diameter of the interference portion is greater than or equal to the inner diameter of the tube body.

[0018] 11. The embryo transfer tube according to item 9 or 10, characterized in that finger sleeves are provided on the fixing ring, and two finger sleeves are provided on opposite sides of the fixing ring; the two finger sleeves are symmetrically arranged and both are fixedly connected to the fixing ring.

[0019] 12. The embryo transfer tube according to item 7, characterized in that a protective plate is provided at the proximal end of the push rod; the radial dimension of the protective plate is larger than the diameter of the push rod.

[0020] 13. The embryo transfer tube according to item 1, characterized in that the needle head comprises a sharp injection head and a nesting part, and the nesting part is used to connect the injection head and the tube body.

[0021] 14. The embryo transfer tube according to item 13 is characterized in that the nested portion includes a sleeve plug and a transition tube, the outer diameter of the transition tube is smaller than the inner diameter of the tube body or the inner diameter of the transition tube is larger than the outer diameter of the tube body, and the sleeve plug is arranged on the outside of the transition tube, and the sleeve plug can tightly clamp the tube body and the transition tube.

[0022] 15. The embryo transfer tube according to item 14, characterized in that the injection head and the transition tube are integrally formed.

[0023] 16. The embryo transfer tube according to item 14 is characterized in that the sleeve plug includes an elastic portion located at the distal end and a fastening portion at the proximal end, the elastic portion is conical with a diameter gradually decreasing toward the distal end; and the fastening portion is columnar.

[0024] 17. The embryo transfer tube according to any one of items 1 to 16, characterized in that a heat preservation device is provided on the outside of the tube body, and the heat preservation device is used to keep the tank body warm.

[0025] 18. The embryo transfer tube according to any one of items 1 to 17, characterized in that the needle head comprises an injection tube and a one-way tube; the one-way tube is arranged between the tube body and the injection tube.

[0026] 19. The embryo transfer tube according to item 18, characterized in that the needle end of the injection tube is a soft tube.

[0027] 20. The embryo transfer tube according to item 18, characterized in that the one-way tube can be connected to the tube body and remain sealed, and the one-way tube is fixed on the injection tube.

[0028] Beneficial effects

[0029] The embryo transfer tube of this application addresses the imperfect in vitro maturation of oocytes, thereby better utilizing embryos, reducing the risks of embryo transfer, and increasing the efficiency of cloned embryo attachment and birth rate. This improves the efficiency of embryo transfer and plays a significant role in the preservation of small and medium-sized animals and the protection of high-quality resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is an exploded view of the embryo transfer tube of this application;

[0031] Figure 2 This is an exploded view of the embryo transfer tube of the present application from another perspective;

[0032] Figure 3 This is an exploded view of the tube body and suction device of the present application;

[0033] Figure 4 It is a schematic diagram of the structure of the injection tube in this application;

[0034] Figure 5 This is the installation structure diagram of the injection tube and the tube body in this application.

[0035] In the figure, 1. tube body; 2. needle head; 3. injection head; 4. sleeve plug; 5. transition tube; 6. injection part; 7. push rod; 8. fixing ring; 9. finger sleeve; 10. protection plate; 11. air cotton core; 12. syringe; 13. connecting tube; 14. injection tube; 15. intermediate tube. DETAILED DESCRIPTION

[0036] The present application is described in detail below. Although specific embodiments of the present application are shown, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope of the present application to those skilled in the art.

[0037] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the difference in the functions of the components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" are open-ended terms and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present application, but the description is based on the general principles of the specification and is not intended to limit the scope of the present application. The scope of protection of this application shall be as defined by the attached claims.

[0038] refer to Figure 1 and Figure 2 The present application provides an embryo transfer tube. The tube body 1 includes a needle head 2 disposed at the distal end of the tube body 1. The needle head 2 is detachably connected to the tube body 1 and is a sharp tip capable of puncture.

[0039] An injection portion 6 is provided at the proximal end of the tubular body 1, and the injection portion 6 is detachably connected to the tubular body 1. A cotton core is provided inside the tubular body 1, and the cotton core can push the liquid inside the tubular body 1 to the needle head 2;

[0040] refer to Figure 3 A suction device is also provided at the proximal end of the tube body 1 , and the suction device is detachably connected to the tube body 1 .

[0041] The embryo transfer tube is used to transfer embryos. Therefore, when the embryo transfer tube is used, the general steps are suction, storage and injection.

[0042] The tube body 1 is a hollow structure, and the embryo can be temporarily stored in the tube body 1 .

[0043] refer to Figure 1 and Figure 3In this application, when using the embryo transfer tube, a suction device is first placed at the proximal end of the tube body 1 and connected to the suction device. The tube body 1 is then placed in a liquid containing embryos, and the suction device is used to draw the liquid containing embryos into the tube body 1. When the liquid is not in contact with the cotton wick, air can pass through the wick, causing the proximal end of the wick to be drawn into a negative pressure state by the suction device. At this point, the liquid containing embryos can be drawn into the tube body 1. Once the liquid contacts the cotton wick, it fills the wick and blocks any gaps within the wick. The liquid is also blocked by the cotton wick. At this point, the dual action of the cotton wick and the liquid temporarily stores the drawn liquid containing embryos in the tube body 1, preventing the suction device from drawing the liquid. Furthermore, the drawn liquid remains in the tube body 1, and due to the sealing effect of the cotton wick and the liquid, it does not move within the tube body 1. This completes the drawing of the liquid containing embryos.

[0044] Then, the tube body 1 that has absorbed the embryonic liquid is moved to the animal or other location to be injected, and the suction device is replaced with the injection part 6, which is used to push the cotton core.

[0045] refer to Figure 1 and Figure 3 Because the cotton core forms a seal with the liquid after contact, in order to expel the embryo from the tube 1, the injection unit 6 is used to completely push the cotton core and the liquid containing the embryo at the front end of the cotton core out of the tube 1. As the injection unit 6 pushes the cotton core toward the proximal end, the cotton core continues to push the liquid out of the proximal end of the tube 1.

[0046] refer to Figure 1 and Figure 3 When an injection is required into an animal or other object, the needle head 2 is mounted on the proximal end of the tube body 1 before the injection unit 6 pushes the cotton core. The tip of the needle head 2 is then inserted into the target location. The injection unit 6 is then used to expel the embryo from the tube body 1 and then transport it to the target location through the needle head 2. This completes the embryo transfer.

[0047] refer to Figure 1 and Figure 3 The present invention provides a cotton core in the tube 1 so that the liquid containing the embryos stops after being sucked into the tube 1 at the position of the cotton core. Thus, on the one hand, the cotton core can control the amount of embryos or liquid sucked into the tube 1 by moving its position; on the other hand, the cotton core seals the tube 1 after contacting the liquid, thereby sealing the liquid sucked into the tube 1 and reducing the possibility of the embryos falling out of the tube 1.

[0048] refer to Figure 1 and Figure 3The present application adds a suction device, which enables the tube body 1 to suck the embryo into the tube body 1 under the action of the suction device. Compared with injecting or delivering the embryo into the tube body 1, the sucked embryo is less likely to be damaged. In addition, the sucked embryo is more easily controlled by the suction device inside the tube body 1, making it easier to place the embryo in the appropriate position.

[0049] The embryo sucked into the tube body 1 is pushed out of the tube body 1 by the injection part 6 , which pushes the cotton core part to expel the cotton core part and the embryo displaced at its proximal end from the tube body 1 , thereby reducing the residual embryo in the tube body 1 .

[0050] refer to Figure 1 and Figure 3 The needle head 2 is fixed at the front end of the tube body 1. The needle head 2 is used to pierce the animal or other object, so that the tube body 1 can be directly injected under the action of the needle head 2. This simplifies the tedious steps of sucking the embryo inside the tube body 1 with the syringe 12 and then injecting it. It greatly increases the efficiency of embryo transplantation from the tube body 1 and also reduces the possibility of embryo damage during the transplantation process.

[0051] refer to Figure 1 and Figure 3 When the suction device sucks the embryo into the tube body 1 at the proximal end, the embryo can enter the tube body 1 from the distal end of the tube body 1, and the position of the cotton core in the tube body 1 remains fixed.

[0052] refer to Figure 3 The suction device is a negative pressure suction device, and the cotton core has a structure with a gap in the middle through which air can pass. Therefore, when the suction device is sucking, the suction device is in a negative pressure state, where air can pass through the gap in the middle of the cotton core, and the liquid containing the embryo can be sucked into the interior of the tube body 1 by the negative pressure device while the cotton core remains stationary.

[0053] At the same time, the cotton core also has a capillary structure. When the absorbed liquid containing the embryo comes into contact with the cotton core, the liquid will penetrate into the cotton core and fill the cotton core, so that a sealing structure is formed between the cotton core and the tube body 1, thereby allowing the liquid containing the embryo to remain in the tube body 1 and stay at the cotton core.

[0054] refer to Figure 3 The suction device includes a syringe 12 and a connecting tube 13 fixed to the syringe 12. The outer diameter of the connecting tube 13 gradually decreases from the proximal end to the distal end. The connecting tube 13 can be inserted into the tube body 1, and the tube body 1 and the connecting tube 13 are kept sealed.

[0055] The syringe 12 is a conventional syringe 12 in the art. The connecting tube 135 is used to connect the syringe 12 to the tubular body 1. The proximal end of the connecting tube 13 is fixedly connected to the distal end of the syringe 12, and the connecting tube 13 and the syringe 12 are sealed. This allows the syringe 12 to create a negative pressure state at the proximal end of the tubular body 1.

[0056] refer to Figure 3 The distal end of the connecting tube 13 is used to connect with the tubular body 1 and allow the syringe 12 to extract the air inside the tubular body 1. Therefore, the sealing between the connecting tube 13 and the tubular body 1 needs to be maintained.

[0057] refer to Figure 3 In this application, the distal end of the connecting tube 13 is configured to have a diameter that gradually decreases from the proximal end to the distal end. This allows the distal end of the connecting tube 13 to gradually enter the interior of the tubular body 1, thereby achieving a close fit and a seal between the outer wall of the connecting tube 13 and the inner wall of the tubular body 1. Subsequently, during aspiration, to reduce the possibility of air leakage between the connecting tube 13 and the tubular body 1, the syringe 12 can be further inserted into the tubular body 1, creating an interference fit between the tubular body 1 and the connecting tube 13. This allows the syringe 12 to more smoothly draw the embryo into the tubular body 1.

[0058] refer to Figure 3 When the embryo is sucked into the tube body 1 using the suction device, the embryo is stopped from being sucked after the liquid containing the embryo contacts the cotton core.

[0059] refer to Figure 3 When the embryo is drawn into the tube 1, the liquid containing the embryo will continue to move proximally within the tube 1 as the suction device draws, until it contacts the cotton wick. The liquid then penetrates the cotton wick, filling it with liquid and forming a seal between the wick and the tube 1. At this point, the suction device draws the embryo from the tube 1 again. Because the liquid in the cotton wick has already been sealed by the tube 1, it is difficult for the liquid in the cotton wick to move within it. Therefore, the suction device cannot draw liquid again after the cotton wick is sealed. At this point, the embryo is completely drawn.

[0060] refer to Figure 1 and Figure 2 The injection part 6 is used to push the cotton core part to move.

[0061] After the embryo is sucked into the tube body 1, the tube body 1 is moved to the position where injection is required, and the embryo can be pushed out of the tube body 1 using the injection part 6. Since the cotton core inside the tube body 1 seals the tube body 1, the injection part 6 directly pushes the cotton core, which continuously pushes the liquid at its proximal end and the embryo inside, thereby completely pushing the embryo out of the tube body 1.

[0062] refer to Figure 1 and Figure 2 The cotton core part is an air cotton core 11. When there are embryonic cells inside the tube body 1, the air cotton core 11 can push the embryonic cells out of the tube body 1.

[0063] refer to Figure 1 and Figure 2 The air cotton core 11 is the cotton core used in the freezing tube in the art. There are gaps inside the air cotton core 11, so that gas can pass through the air cotton core 11.

[0064] In addition, the gaps inside the air cotton core 11 can allow air to pass through while also blocking the flow of liquid. When the liquid flows to the position of the air cotton core 11, the gaps inside the air cotton core 11 will be absorbed into the air cotton core 11 under the action of capillary phenomenon.

[0065] refer to Figure 1 and Figure 2 Therefore, when embryonic cells and liquid are stored inside the tube body 1, the air wick 11 will move toward the proximal end, expelling the air inside the tube body 1 to the proximal end of the air wick 11 until the air wick 11 contacts the embryonic cells and liquid. The liquid fills the gap in the air wick 11, forming a seal between the air wick 11 and the tube body 1. When the air wick 11 is then pushed, the air wick 11, now sealed with the tube body 1, will simultaneously push the embryonic cells inside the tube body 1 to move. Furthermore, because the air wick 11 is made of a relatively soft material and uses liquid for sealing, the damage caused to the embryonic cells by the air wick 11 when in contact with them is greatly reduced. This ensures that the embryonic cells inside the tube body 1 remain sufficiently intact, reducing the possibility of damage to the embryo during transplantation.

[0066] refer to Figure 1 and Figure 2 The injection part 6 includes a push rod 7, which is arranged inside the tube body 1 and can move back and forth inside the tube body 1.

[0067] The push rod 7 is a cylindrical structure, is located inside the tube body 1, and the length of the push rod 7 is adapted to the length of the tube body 1. Preferably, the length of the push rod 7 is slightly smaller than the length of the tube body 1.

[0068] When the air wick 11 needs to be pushed, the push rod 7 can be placed inside the tube body 1 at the proximal end of the tube body 1, and the air wick 11 can be pushed by pushing the push rod 7. The air wick 11 can push the embryonic cells and liquid inside the tube body 1 out of the tube body 1.

[0069] refer to Figure 1 and Figure 2The injection part 6 also includes a fixing part, which can be fixed to the proximal end of the tube body 1.

[0070] refer to Figure 1 and Figure 2 The fixing portion is disposed at the proximal end of the tube body 1, close to the operator's position. The fixing portion can be fixed to the tube body 1. When the push rod 7 is used to push the air wick 11 inside the tube body 1, the fixing portion can fix the tube body 1, thereby reducing the possibility that the tube body 1 moves synchronously with the air wick 11 when the push rod 7 pushes the air wick 11, so that the push rod 7 can push the air wick 11 to move inside the tube body 1.

[0071] On the other hand, the operator can also use the fixed part to gain leverage. When the operator pushes the push rod 7, the air cotton core 11 and the tube body 1 are in a sealed state, and there are embryonic cells and liquid inside the tube body 1. Therefore, a certain amount of power is required to push the air cotton core 11. Therefore, when the operator finds it difficult to push the push rod 7 to move the air cotton core 11, the operator can use his fingers or other components to act on the fixed part to partially disperse the force of pushing the push rod 7 to the fixed part, so that the fixed part can provide a certain pulling force for the push rod 7, thereby enabling the operator to use the push rod 7 to push the air cotton core 11 more conveniently.

[0072] refer to Figure 1 and Figure 2 The fixing portion includes a fixing ring 8, which is annular; the inner diameter of the fixing ring 8 is less than or equal to the outer diameter of the tube body 1, and the fixing ring 8 is connected to the tube body 1 by interference fit.

[0073] refer to Figure 1 and Figure 2 The fixing ring 8 is used to fix the pipe body 1. When the fixing ring 8 and the pipe body 1 have an interference fit, the inner diameter of the fixing ring 8 can be slightly larger than the outer diameter of the pipe body 1, so that the pipe body 1 or the fixing ring 8 can undergo a certain elastic deformation, thereby allowing the fixing ring 8 to be sleeved and fixed on the outside of the pipe body 1. Alternatively, the fixing ring 8 includes a stepped interference portion; the inner diameter of the pipe body 1 is slightly smaller than the outer diameter of the connection point between the fixing ring 8 and the pipe body 1, that is, the outer diameter of the interference portion is larger than the inner diameter of the pipe body 1, thereby allowing the pipe body 1 to be sleeved on the outside of the interference portion.

[0074] refer to Figure 1 and Figure 2 The tube body 1 and the fixing ring 8 are both made of a material with a certain elasticity, such as plastic or polyethylene. Therefore, the interference fit between the fixing ring 8 and the tube body 1 not only achieves a fixed connection between the tube body 1 and the fixing ring 8, but also allows the fixing ring 8 to be removed from the tube body 1 within a certain elastic range, thus achieving a detachable connection between the tube body 1 and the fixing ring 8.

[0075] In one embodiment, reference Figure 1 and Figure 2 The fixing portion includes an interference portion (not shown) and a fixing ring 8. The fixing ring 8 is coaxially arranged on the proximal outer side of the interference portion and is integrally formed. The outer diameter of the interference portion is greater than or equal to the inner diameter of the tube body 1. At this time, the tube body 1 is interference-fitted on the outer side of the interference portion.

[0076] refer to Figure 1 and Figure 2 In another specific embodiment, the fixing portion includes a fixing ring 8. At this time, the fixing ring 8 is sleeved on the outside of the tube body 1.

[0077] refer to Figure 1 and Figure 2 A finger sleeve 9 is provided on the fixing ring 8 , and two finger sleeves 9 are provided on opposite sides of the fixing ring 8 ; the two finger sleeves 9 are symmetrically arranged and are both fixedly connected to the fixing ring 8 .

[0078] refer to Figure 1 and Figure 2 Finger cuff 9 is fixed to fixing ring 8. Once fixing ring 8 is installed on tube body 1, finger cuff 9 is also fixedly connected to tube body 1. Therefore, when pushing push rod 7, the operator can place a finger in finger cuff 9 and use finger cuff 9 to apply the force of pushing push rod 7 to fixing ring 8. This makes it easier for the operator to apply force to push rod 7. At the same time, after the operator places a finger in finger cuff 9, the operator can also use finger cuff 9 to move and fix the tube body, thereby moving the tube body to a convenient position and fixing it. This improves the convenience of tube body 1 and embryo transplantation.

[0079] refer to Figure 1 and Figure 2 A protective plate 10 is provided at the proximal end of the push rod 7 ; the radial dimension of the protective plate 10 is larger than the diameter of the push rod 7 .

[0080] The protection plate 10 is plate-shaped and can be circular, square, triangular or other shapes, preferably circular.

[0081] refer to Figure 1 and Figure 2 When the operator uses the push rod 7 to push the air cotton core 11, the operator can press the proximal end of the push rod 7 with his fingers, thereby pushing the push rod 7 to move inside the tube body 1.

[0082] refer to Figure 1 and Figure 2 The diameter of the protective plate 10 is larger than the diameter of the push rod 7, and at the same time, the surface area of ​​the protective plate 10 is larger than the cross-sectional area of ​​the push rod 7, thereby increasing the contact area between the human hand and the protective plate 10, thereby reducing the pressure received by the operator's fingers and reducing the damage to the operator's hands.

[0083] In addition, the radial dimension of the protective plate 10 is larger than the radial dimension of the tube body 1. When the push rod 7 is used to push the air cotton core 11, the protective plate 10 can prevent the push rod 7 from entering the interior of the tube body 1 as a whole. Therefore, after pushing the air cotton core 11, the push rod 7 can be smoothly removed from the tube body 1, reducing the possibility that the push rod 7 will completely enter the tube body 1 and become difficult to remove.

[0084] refer to Figure 1 and Figure 2 The needle head 2 includes a sharp injection head 3 and a nesting portion, and the nesting portion is used to connect the injection head 3 and the tube body 1.

[0085] refer to Figure 1 and Figure 2 The injection head 3 is used for puncture and injection. When it is necessary to inject embryos into the animal body, the injection head 3 can pierce the skin and enter the target position in the animal body, and then the embryos inside the tube body 1 can be injected into the animal body.

[0086] The sharp portion of the injection head 3 is used to pierce the skin and internal tissues of the animal, thereby delivering the tip of the injection head 3 to a target location in the animal's body.

[0087] refer to Figure 1 and Figure 2 The nesting portion is used to fix the injection head 3 to the tube body 1. The injection head 3 is a hollow cylindrical shape, and the outer diameter of the injection head is slightly smaller than the inner diameter of the tube body. Therefore, when the injection head 3 and the tube body are installed, the tube body is placed on the outside of the injection head 3, and the injection head 3 and the tube body are interference fit to achieve the connection between the injection head 3 and the tube body.

[0088] refer to Figure 1 and Figure 2 When using the injection head 3 for injection, in order to improve the connection strength and sealing effect between the injection head 3 and the tube body, the nesting portion is moved to the connection between the injection head 3 and the tube body, and then the nesting portion is used to clamp the injection head 3 and the tube body, thereby improving the connection strength between the tube body and the injection head 3. At the same time, it can also improve the sealing between the tube body and the injection head 3, thereby reducing the possibility of liquid leakage from the connection between the tube body and the injection head 3 when injecting embryos into animals, thereby improving the success rate of embryo transfer.

[0089] refer to Figure 1 and Figure 2 In a specific embodiment, the nested portion includes a sleeve plug 4 and a transition pipe 5, the outer diameter of the transition pipe 5 is smaller than the inner diameter of the tube body 1 or the inner diameter of the transition pipe 5 is larger than the outer diameter of the tube body 1, and the sleeve plug 4 is arranged on the outside of the transition pipe 5, and the sleeve plug 4 can tightly clamp the tube body 1 and the transition pipe 5.

[0090] refer to Figure 1 and Figure 2 When the size of the injection head 3 used is difficult to adapt to the size of the tube body 1, for example, when a smaller diameter injection head 3 is used for smaller animals, or when a larger diameter injection head 3 is used for embryo transplantation of larger animals, it is difficult to directly connect the injection head 3 and the tube body. In this case, a transition tube 5 can be used to connect the injection head 3 and the tube body respectively, thereby achieving the connection between the injection head 3 and the tube body.

[0091] The size of the transition pipe 5 is adapted to the size of the injection head 3 , so that the transition pipe 5 can be connected to the injection head 3 and the connection strength and sealing between the transition pipe 5 and the injection head 3 are maintained.

[0092] refer to Figure 1 and Figure 2 After the transition tube 5 is connected to the injection head 3, it is then connected to the tube body. After the transition tube 5, which matches the size of the injection head 3, is connected to the tube body, a sleeve plug 4 is used to reinforce the connection between the tube body and the transition tube 5. The sleeve plug 4 allows elastic deformation between the tube body or the transition tube 5, thereby strengthening the connection between the tube body and the transition tube 5. It also improves the sealing between the tube body and the transition tube 5, reducing the possibility of liquid leakage between the tube body and the transition tube 5.

[0093] refer to Figure 1 and Figure 2 In a specific embodiment, the injection head 3 and the transition pipe 5 are integrally formed.

[0094] The injection head 3 and the transition pipe 5 are integrally formed, thereby strengthening the connection strength between the transition pipe 5 and the injection head 3 and further improving the sealing between the injection head 3 and the transition pipe 5.

[0095] refer to Figure 1 and Figure 2 The sleeve plug 4 includes an elastic portion at the distal end and a fastening portion at the proximal end. The elastic portion is conical with a diameter gradually decreasing toward the distal end; the fastening portion is columnar.

[0096] refer to Figure 1 and Figure 2 The sleeve plug 4 is used to connect the transition pipe 5 and the pipe body 1. Therefore, after the sleeve plug 4 is sleeved on the pipe body 1 and the transition pipe 5, the sleeve plug 4 can tighten the pipe body 1 or the transition pipe 5, and cause the pipe body 1 or the transition pipe 5 to undergo a certain amount of elastic deformation inward, thereby making the transition pipe 5 and the pipe body 1 in close contact to complete the sealing between the transition pipe 5 and the pipe body 1.

[0097] refer to Figure 1 and Figure 2The fastening portion is cylindrical, and the inner diameter of the fastening portion is smaller than the outer diameter of the transition pipe 5 or the pipe body 1 , so the fastening portion can achieve a tight and sealed connection between the transition pipe 5 and the pipe body 1 .

[0098] refer to Figure 1 and Figure 2 The elastic portion is the part with greater elasticity. The elastic portion and the fastening portion are integrally formed. Therefore, when the sleeve plug 4 is inserted into the pipe body 1 and the transition pipe 5, the elastic portion can be expanded, thereby increasing the diameter of the elastic portion. Then, the elastic portion and the fastening portion are sleeved onto the pipe body 1 and the transition pipe 5. This reduces the difficulty of installing the sleeve plug 4.

[0099] refer to Figure 4 and Figure 5 In another specific embodiment, the needle head 2 includes an injection tube 14 and a one-way tube 15 ; the one-way tube 15 is arranged between the tube body 1 and the injection tube 14 .

[0100] In another specific embodiment, an intermediate tube is provided between the injection tube 14 and the tube body 1, and the intermediate tube is fixedly connected to the injection tube 14. The side of the intermediate tube close to the tube body 1 is a central opening and has an elastic structure, so that the intermediate tube can be connected to tube bodies 1 of various sizes, thereby improving the applicability of the intermediate tube.

[0101] refer to Figure 4 and Figure 5 The injection tube 14 is used to be inserted into the animal body, and then the embryo in the tube body 1 is injected into the target position in the animal body through the injection tube 14.

[0102] refer to Figure 4 and Figure 5 A one-way tube 15 is connected between the syringe 14 and the tube body 1. The one-way tube 15 is a pipe that can only allow liquid to flow from the tube body 1 to the syringe 14. The liquid in the syringe 14 cannot flow through the one-way tube 15 into the tube body 1, thereby reducing the possibility of blood backflow or backflow when the syringe 14 is in the animal's body. This improves the safety of injecting liquid into the animal's body using the syringe 14 through the tube body 1.

[0103] refer to Figure 4 and Figure 5 In one embodiment, syringe 14 is an indwelling needle. Once inserted into the animal, syringe 14 can remain temporarily within the animal. This allows the surgeon to continue injecting other substances into the animal after injecting the embryo or fluid into the tube body 1. This improves the margin for error during surgery and, in turn, increases the success rate of the procedure.

[0104] refer to Figure 4 and Figure 5The needle end of the injection tube 14 is a soft tube.

[0105] The needle end of the syringe 14 is the portion that penetrates the animal's body. By configuring the needle end as a flexible tube, once inside the animal, the needle end can be less likely to injure the animal's body as it moves. Therefore, the flexible tube-shaped needle end can remain temporarily within the animal's body, minimizing the impact on the animal's movement and daily life. When further injections of medication or other substances are needed, the syringe 14 can be used directly, reducing the need for multiple punctures and the likelihood of the animal struggling during the procedure.

[0106] refer to Figure 4 and Figure 5 It is worth mentioning that when injecting embryos into an animal, the syringe 14 is inserted into the animal's oviduct and then the embryo is injected therein. The animal's oviduct is extremely fragile, so the needle tip is configured as a flexible tube structure to reduce damage to the animal's oviduct by the syringe 14, thereby reducing harm to the animal and improving the success rate of embryo transplantation.

[0107] refer to Figure 4 and Figure 5 The one-way tube 15 can be connected to the tube body 1 and remain sealed, and the one-way tube 15 is fixed on the injection tube 14.

[0108] refer to Figure 4 and Figure 5 The one-way tube 15 is used to connect the injection tube 14 with the tube body 1. In different usage environments, the size of the tube body 1 may change, making it difficult to connect the tube body 1 and the injection tube 14 during installation. Therefore, the one-way tube 15 is used to connect the tube body 1 and the injection tube 14. One side of the one-way tube 15 is fixedly connected to the injection tube 14. The side of the one-way tube 15 close to the tube body 1 is an elastic tube, and the diameter of the one-way tube 15 gradually decreases from the end to the middle. Therefore, the one-way tube 15 can be connected to tube bodies 1 of more sizes, and tube bodies 1 of various sizes can enter the interior of the one-way tube 15 along the tapered end until a sealed and fixed connection is achieved with the one-way tube 15.

[0109] refer to Figure 1 and Figure 5 A heat preservation device (not shown in the figure) is provided on the outside of the tube body 1, and the heat preservation device is used to keep the tank body warm.

[0110] The heat preservation device is used to keep tube 1 warm. During embryo transfer surgery, embryos are cultured inside the operating room, but the animal undergoing the embryo transfer is not living in the operating room. Therefore, after using tube 1 to absorb the embryonic cells, tube 1 is moved to a fixed position on the animal before injection into the animal. During the movement of tube 1 containing the embryonic fluid, the heat preservation device is used to keep the embryo at an appropriate survival temperature while moving within tube 1, in order to reduce the impact of the external environment on the survival of the embryonic cells and improve the survival rate of the embryo. This reduces embryonic cell death caused by temperature increases or decreases, thereby improving the success rate of embryo transfer surgery.

[0111] In one embodiment, the heat preservation device is a thermostat. The thermostat is used to control the temperature of the tube 1. When the tube 1 is moved, the tube 1 is directly placed in the thermostat. The thermostat isolates the tube 1 from the external environment, thereby reducing the impact of the external environment on the embryos in the tube 1.

[0112] In summary, the present application provides an embryo transplant needle. When performing an embryo transplant operation, it is necessary to use a suction device to suck the liquid containing the embryo into the tube body 1. First, the air cotton core 11 is placed inside the tube body 1. According to the actual situation, the air cotton core 11 is placed in a preset fixed position. The position of the air cotton core 1 is related to the liquid volume of the embryo to be sucked and the number of embryonic cells. Then, the connecting tube 13 is used to connect to the tube body 1, and at the same time, the syringe 12 is fixedly connected to the connecting tube 13. At this time, the needle head 2 can be connected to the tube body 1 and the needle head 2 can be used to suck the embryo, or the tube body 1 can be used directly to suck the embryo. The needle head 2 is placed in the liquid containing the embryo, and then the syringe 12 is used to suck it, so that the liquid containing the embryo will be sucked into the tube body 1. Until the liquid contacts the air cotton core 11, and then under the capillary phenomenon of the air cotton core 1, the liquid quickly permeates the air cotton core 11, thereby achieving a seal between the air cotton core 11 and the tube body 1. At this time, because the soaked air cotton wick 11 blocks one end of the tube body 1, the embryonic cells and liquid sucked into the tube body 1 will also remain in the tube body 1 under the action of atmospheric pressure. Then, the connecting tube 13 and syringe 12 are removed, and the tube body 1 is placed in an incubator for insulation. At the same time, the surgeon takes the incubator and the tube body 1 inside it to the animal where the embryo is to be transferred.

[0113] To transplant an embryo into an animal, first install the needle head 2 onto the tube body 1. Then, install the injection unit 6 at the proximal end of the tube body 1, secure the retaining ring 8 to the tube body 1, and bring the push rod 7 into contact with the air wick 11. The needle head 2 is then inserted into the animal's body, ensuring that the tip of the needle head 2 reaches the target location within the animal. The operator then inserts their finger into the fingertip 9 of the injection unit 6 and uses the push rod 7 to push the air wick 11, which in turn pushes the embryo inside the tube body 1 into the animal's body. This completes the embryo transplant procedure.

[0114] When installing the needle head 2 , when the needle head is an injection head 3 , the injection head 3 is connected to the tube body 1 using the nesting portion, and the injection head 3 and the tube body 1 are tightened and fixed.

[0115] When the needle head 2 is a syringe 14, the needle end of the syringe 14 is a soft tube, so an auxiliary rigid needle is needed to puncture the animal skin or tissue, and the needle of the syringe 14 is transported to the target position, and then the auxiliary rigid needle is withdrawn to complete the insertion of the syringe 14.

[0116] Example

[0117] This application provides an embryo transfer tube. The tube body 1 is 8-12 cm long and 1.9-2 mm wide. The injection head 3 is smaller than the fallopian tube but larger than the early embryo. Preferably, the needle of the injection head 3 is a 0.9 mm x 37 mm needle. The transition tube 5 and the injection head 3 are integrally formed. The sleeve 4 is used to secure the transition tube 5 to the tube body 1.

[0118] In this embodiment, the experimental subjects are Bama pigs. Embryo transplantation is performed on Bama pigs, and the cultured embryonic cells are transplanted into the Bama pigs.

[0119] The air cotton core 11 used in the cotton core part is the air cotton core 11 inside the French Casu beef jelly fine tube.

[0120] The suction device is a syringe 12, specifically, the suction device is a 1 ml syringe 12. The connector is made of plastic.

[0121] The heat preservation device is a constant temperature box.

[0122] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. An embryo transfer tube, characterized in that: The device comprises a tube body, a needle head is provided at the distal end of the tube body, the needle head is detachably connected to the tube body, and the needle head is a sharp tip capable of puncture; An injection portion is provided at the proximal end of the tube body, the injection portion being detachably connected to the tube body, and a cotton core portion being provided inside the tube body, the cotton core portion being capable of pushing the liquid inside the tube body to the needle head; A suction device is also provided at the proximal end of the tube body, and the suction device is detachably connected to the tube body.

2. The embryo transfer tube according to claim 1, characterized in that: When the suction device sucks the embryo into the tube body at the proximal end, the embryo can enter the tube body from the distal end of the tube body, and the position of the cotton core in the tube body remains fixed.

3. The embryo transfer tube according to claim 1, characterized in that: The suction device includes a syringe and a connecting tube fixed to the syringe. The outer diameter of the connecting tube gradually decreases from the proximal end to the distal end. The connecting tube can be inserted into the tube body, and the tube body and the connecting tube are kept sealed.

4. The embryo transfer tube according to claim 1, characterized in that: When the embryo is sucked into the tube body using the suction device, the suction of the embryo is stopped after the liquid containing the embryo contacts the cotton core.

5. The embryo transfer tube according to claim 1, characterized in that: The injection part is used to push the cotton core part to move.

6. The embryo transfer tube according to claim 1, characterized in that: The cotton core is an air cotton core. When embryonic cells are present in the tube body, the air cotton core can push the embryonic cells out of the tube body.

7. The embryo transfer tube according to claim 1, characterized in that: The injection part includes a push rod, which is arranged inside the tube body and can move back and forth inside the tube body.

8. The embryo transfer tube according to claim 7, characterized in that: The injection part further includes a fixing part, which can be fixed to the proximal end of the tube body.

9. The embryo transfer tube according to claim 8, characterized in that: The fixing portion includes a fixing ring, which is annular; the inner diameter of the fixing ring is smaller than or equal to the outer diameter of the tube body, and the fixing ring is connected to the tube body through interference fit.

10. The embryo transfer tube according to claim 8, characterized in that: The fixing portion includes an interference portion and a fixing ring. The fixing ring is coaxially arranged on the outer side of the proximal end of the interference portion and is integrally formed. The outer diameter of the interference portion is greater than or equal to the inner diameter of the tube body.

11. The embryo transfer tube according to claim 9, characterized in that: Finger sleeves are arranged on the fixing ring, and two finger sleeves are arranged on opposite sides of the fixing ring; the two finger sleeves are symmetrically arranged and both are fixedly connected to the fixing ring.

12. The embryo transfer tube according to claim 7, characterized in that: A protection plate is provided at the proximal end of the push rod; the radial dimension of the protection plate is larger than the diameter of the push rod.

13. The embryo transfer tube according to claim 1, characterized in that: The needle head comprises a sharp injection head and a nesting portion, and the nesting portion is used to connect the injection head and the tube body.

14. The embryo transfer tube according to claim 13, characterized in that: The nested portion includes a sleeve plug and a transition pipe, the outer diameter of the transition pipe is smaller than the inner diameter of the pipe body or the inner diameter of the transition pipe is larger than the outer diameter of the pipe body, the sleeve plug is arranged on the outside of the transition pipe, and the sleeve plug can tightly clamp the pipe body and the transition pipe.

15. The embryo transfer tube according to claim 14, characterized in that: The injection head and the transition pipe are integrally formed.

16. The embryo transfer tube according to claim 14, characterized in that: The sleeve plug comprises an elastic portion at a distal end and a fastening portion at a proximal end. The elastic portion is tapered with a diameter gradually decreasing toward the distal end; the fastening portion is columnar.

17. The embryo transfer tube according to any one of claims 1 to 16, characterized in that: A heat preservation device is provided on the outside of the tube body, and the heat preservation device is used to keep the tank body warm.

18. The embryo transfer tube according to any one of claims 1 to 16, characterized in that: The needle head comprises an injection tube and a one-way tube; the one-way tube is arranged between the tube body and the injection tube.

19. The embryo transfer tube according to claim 18, characterized in that: The needle end of the injection tube is a soft tube.

20. The embryo transfer tube according to claim 18, characterized in that: The one-way tube can be connected to the tube body and kept sealed, and the one-way tube is fixed on the injection tube.

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