Plastic staining jar for fluorescence in-situ hybridization experiment

Abstract

The utility model discloses a plastic staining jar for fluorescence in situ hybridization experiments, which relates to the technical field of fluorescence in situ hybridization experiments and comprises a staining jar body, a liquid inlet is arranged on the rear side of the upper surface of the staining jar body, and a liquid storage tank is arranged inside the staining jar body. A water level monitoring mechanism is arranged on the right side of the upper surface of the dyeing vat body, a liquid inlet groove is formed in the lower end of the water level monitoring mechanism, a cylinder is slidably arranged in the water level monitoring mechanism, a dial gauge is arranged on the outer rod face of the water level monitoring mechanism, and a liquid outlet is formed in the right side of the outer surface of the dyeing vat body. Compared with an existing common plastic staining jar for the fluorescence in-situ hybridization experiment, the plastic staining jar for the fluorescence in-situ hybridization experiment has the advantages that the amount of liquid entering the staining jar is determined through a dial gauge outside the water level monitoring mechanism, and the height of the liquid in the staining jar is extremely convenient to observe; and no light source can enter the dyeing cylinder, so that the experiment accuracy is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence in situ hybridization experiments, in particular to a plastic staining jar used for fluorescence in situ hybridization experiments. Background Art

[0002] Fluorescence in situ hybridization (FISH) is a non-radioactive molecular cytogenetic technique developed in the late 1980s based on radioactive in situ hybridization (RISH). It replaces isotopic labeling with fluorescent markers, forming a new in situ hybridization method. The probe is first bound to a mediating molecule, and after hybridization, it is linked to a fluorescent dye through an immunocytochemical process. The basic principle of FISH is to label a DNA probe with a specific nucleotide molecule. The probe is then directly hybridized to chromosomes or DNA fiber sections. Monoclonal antibodies conjugated to fluorescein molecules are then used to specifically bind to the probe molecules to detect the qualitative, localized, and relatively quantitative DNA sequences on chromosomes or DNA fiber sections. FISH offers advantages such as safety, rapidity, high sensitivity, long-term probe storage, and the ability to display multiple colors simultaneously. It can visualize not only metaphase nuclei but also interphase nuclei. Multi-color FISH and chromatin fiber FISH have also been developed based on FISH. FISH experiments require multiple washing and dehydration steps, and experiments require the use of staining jars. After hybridization, subsequent washes on slides must be protected from light.

[0003] For example, the public document with application number 202223445797.5 discloses a plastic staining jar for fluorescence in situ hybridization experiments. The advantage of this plastic staining jar for fluorescence in situ hybridization experiments is that it can optimize the light-shielding conditions of fluorescence in situ hybridization experiments, thereby reducing the impact of the environment on probe fluorescence quenching. However, this plastic staining jar for fluorescence in situ hybridization experiments does not consider providing a detachable baffle at the observation port, which makes it extremely inconvenient to observe the water level during the experiment, and when it is opened, light will enter, which will affect the experimental results.

[0004] Therefore, in view of this, the shortcomings of the existing structure are studied and improved, and a plastic staining jar for fluorescence in situ hybridization experiments is proposed. Utility Model Content

[0005] The purpose of the utility model is to provide a plastic staining jar for fluorescence in situ hybridization experiments to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solution: a plastic staining jar for fluorescence in situ hybridization experiments, comprising a staining jar body, a liquid inlet is provided on the rear side of the upper surface of the staining jar body, a liquid storage tank is provided inside the staining jar body, a water level monitoring mechanism is provided on the right side of the upper surface of the staining jar body, a liquid inlet groove is provided at the lower end of the water level monitoring mechanism, a cylinder is provided for sliding inside the water level monitoring mechanism, and a scale is provided on the external rod surface of the water level monitoring mechanism, and a liquid outlet is provided on the right side of the outer surface of the staining jar body.

[0007] Preferably, a second connection block is fixedly provided on the left and right sides of the outer surface of the dyeing tank body, and a thread groove is provided on the upper surface of the second connection block.

[0008] Preferably, a glass socket is provided on the upper surface of the dyeing jar body, and a sealing gasket is provided inside the glass socket.

[0009] Preferably, a cover plate is fixedly provided on the upper surface of the sealing gasket, connecting blocks 1 are fixedly provided on the left and right sides of the surface of the cover plate, and a lifting rod is fixedly provided at the center of the upper surface of the cover plate.

[0010] Preferably, a threaded rod is provided on the upper surface of the connecting block 1, and the threaded rod passes through the connecting block 1.

[0011] Preferably, universal wheels are provided at the four corners of the lower surface of the dyeing vat body, and bolts are fixedly provided on the left and right sides of the outer surface of the dyeing vat body, and the bolts pass through a connecting plate.

[0012] Preferably, a connecting column is fixedly provided on the upper end of one side of the opposite surface of the connecting plate, and a rubber grip is fixedly provided at the center of the outer surface of the connecting column.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. The utility model is provided with a staining vat body, a liquid inlet, a liquid reservoir, a water level monitoring mechanism, a liquid inlet groove, a cylinder and a liquid outlet. Before conducting a fluorescence in situ hybridization experiment, the experimental liquid is discharged into the liquid reservoir in the staining vat body through the liquid inlet. At this time, the lower part of the water level monitoring mechanism is located in the liquid reservoir. Liquid enters the liquid inlet groove on the water level monitoring mechanism, and the cylinder rises due to the buoyancy generated by the liquid. At this time, the position of the upper end of the cylinder in the water level monitoring mechanism is observed, and the amount of liquid entering is determined by a scale on the outside of the water level monitoring mechanism. After the experiment is completed, the liquid is discharged through the liquid outlet to prevent internal corrosion caused by long-term storage. This arrangement is extremely convenient for observing the height of the liquid inside the staining vat, and no light source can enter the staining vat, which increases the accuracy of the experiment.

[0015] 2. The utility model is provided with a dyeing vat body, a threaded groove, a second connecting block, a cover plate, a glass socket, a sealing gasket, a first connecting block, a lifting rod and a threaded rod. When conducting an experiment, the threaded rod on the first connecting block is rotated to remove the threaded rod from the threaded groove on the second connecting block. Then, the experimenter holds the lifting rod to lift the cover plate, and the sealing gasket under the cover plate is removed from the glass socket. The experimenter places a glass slide in the glass socket, and then fixes the cover plate to the dyeing vat body through the same steps. This arrangement can fix the cover plate to the dyeing vat body by providing a fixing mechanism, thereby preventing the cover plate from falling due to impact and shaking during the experiment and spilling liquid, which would cause unnecessary economic losses.

[0016] 3. The utility model is provided with a universal wheel, a dyeing vat body, a connecting column, a connecting plate and a rubber grip. When the dyeing vat body needs to be moved, the experimenter holds the rubber grip connected by the connecting column and the connecting plate to drive the universal wheel below to move the dyeing vat body. The rubber grip can protect the experimenter's hands and prevent hand injuries. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the structure of the dyeing tank body of the utility model;

[0019] Figure 3 This is a schematic diagram of the cover structure of the utility model;

[0020] Figure 4 This is a schematic diagram of the cross-sectional structure of the dyeing vat body of the utility model;

[0021] Figure 5 This is a schematic cross-sectional view of the water level monitoring mechanism of the utility model.

[0022] In the figure: 1. Dyeing jar body; 2. Liquid inlet; 3. Liquid storage tank; 4. Water level monitoring mechanism; 5. Liquid inlet tank; 6. Cylinder; 7. Liquid outlet; 8. Glass socket; 9. Sealing gasket; 10. Cover plate; 11. Lifting rod; 12. Threaded rod; 13. Connecting block 1; 14. Connecting block 2; 15. Threaded groove; 16. Bolt; 17. Connecting plate; 18. Connecting column; 19. Rubber grip; 20. Universal wheel. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] like Figure 1-Figure 5 As shown, a plastic staining jar for fluorescence in situ hybridization experiments comprises a staining jar body 1, a liquid inlet 2 is provided on the rear side of the upper surface of the staining jar body 1, a liquid storage tank 3 is provided inside the staining jar body 1, a water level monitoring mechanism 4 is provided on the right side of the upper surface of the staining jar body 1, a liquid inlet groove 5 is provided at the lower end of the water level monitoring mechanism 4, a cylinder 6 is provided for sliding inside the water level monitoring mechanism 4, and a scale is provided on the rod surface of the water level monitoring mechanism 4 located on the outside, a liquid outlet 7 is provided on the right side of the outer surface of the staining jar body 1, and before conducting the fluorescence in situ hybridization experiment, the experimental liquid is poured into the liquid through the liquid inlet 2. The liquid is discharged into the liquid storage tank 3 in the dyeing vat body 1. At this time, the lower part of the water level monitoring mechanism 4 is located in the liquid storage tank 3, and the liquid enters the liquid inlet groove 5 on the water level monitoring mechanism 4. The cylinder 6 rises due to the buoyancy generated by the liquid. At this time, the position of the upper end of the cylinder 6 in the water level monitoring mechanism 4 is observed, and the amount of liquid entering is determined by the scale outside the water level monitoring mechanism 4. After the experiment is completed, the liquid is discharged through the liquid outlet 7 to prevent internal corrosion caused by long-term storage. This setting is extremely convenient for observing the height of the liquid inside the dyeing vat, and no light source can enter the dyeing vat, which increases the accuracy of the experiment.

[0025] like Figure 1-Figure 3 As shown, the outer surface of the dyeing tank body 1 is fixedly provided with connecting blocks 2 14 on the left and right sides, and the upper surface of the connecting block 2 14 is provided with a threaded groove 15. The upper surface of the dyeing tank body 1 is provided with a glass socket 8, and the interior of the glass socket 8 is provided with a sealing gasket 9. The upper surface of the sealing gasket 9 is fixedly provided with a cover plate 10. The left and right sides of the surface of the cover plate 10 are fixedly provided with connecting blocks 13. The center of the upper surface of the cover plate 10 is fixedly provided with a lifting rod 11. The upper surface of the connecting block 13 is threaded with a threaded rod 12, and the threaded rod 12 passes through the connecting block 13. When conducting the experiment, the screw Turn the threaded rod 12 on the connecting block 13 and take it out of the threaded groove 15 on the connecting block 2 14. Then the experimenter holds the lifting rod 11 to lift the cover 10. The sealing gasket 9 under the cover 10 is removed from the glass socket 8. The experimenter puts the glass slide in the glass socket 8 and then fixes the cover 10 on the dyeing tank body 1 through the same steps. This setting can fix the cover 10 on the dyeing tank body 1 by setting a fixing mechanism to prevent the cover 10 from falling and liquid spilling due to impact and shaking during the experiment, causing unnecessary economic losses.

[0026] like Figures 1-4 As shown, universal wheels 20 are provided at the four corners of the lower surface of the dyeing vat body 1, and bolts 16 are fixedly provided on the left and right sides of the outer surface of the dyeing vat body 1. The bolts 16 pass through a connecting plate 17, and a connecting column 18 is fixedly provided on the upper end of the opposite side of the connecting plate 17. A rubber grip 19 is fixedly provided at the center of the outer surface of the connecting column 18. When the dyeing vat body 1 needs to be moved, the experimenter holds the rubber grip 19 connected to the connecting plate 17 through the connecting column 18 to drive the universal wheel 20 below to move and transport the dyeing vat body 1. The rubber grip 19 can protect the experimenter's hands and prevent hand injuries.

[0027] Working principle: When using this plastic staining jar for fluorescence in situ hybridization experiments, first, before the experiment, when the staining jar body 1 needs to be moved, the experimenter holds the rubber handle 19 connected to the connecting plate 17 through the connecting column 18 to drive the universal wheel 20 below to move the staining jar body 1, and then discharges the experimental liquid into the liquid storage tank 3 in the staining jar body 1 through the liquid inlet 2. At this time, the lower part of the water level monitoring mechanism 4 is located in the liquid storage tank 3, and the liquid enters the liquid inlet groove 5 on the water level monitoring mechanism 4. The cylinder 6 rises due to the buoyancy generated by the liquid. At this time, the position of the upper end of the cylinder 6 in the water level monitoring mechanism 4 is observed. By comparing with the water level monitoring mechanism 4, the experimental liquid is discharged into the liquid storage tank 3. The scale on the outside of the mechanism 4 determines the amount of liquid entering. When conducting the experiment, the threaded rod 12 on the connecting block 13 is rotated to remove the threaded rod 12 from the threaded groove 15 on the connecting block 2 14. Then the experimenter holds the lifting rod 11 to lift the cover 10, and the sealing gasket 9 under the cover 10 is removed from the glass socket 8. The experimenter places the glass slide in the glass socket 8, and then fixes the cover 10 on the staining tank body 1 through the same steps. Finally, after the experiment is completed, the liquid is discharged through the liquid outlet 7 to prevent internal corrosion caused by long-term storage. This is the working principle of this plastic staining tank used for fluorescence in situ hybridization experiments.

[0028] The examples of the present invention are provided for purposes of illustration and description and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The examples are selected and described to better illustrate the principles and practical applications of the present invention and to enable those skilled in the art to understand the present invention and design various embodiments with various modifications suitable for specific applications.

Claims

1. A plastic staining jar for fluorescence in situ hybridization experiments, comprising a staining jar body (1), characterized in that: A liquid inlet (2) is provided on the rear side of the upper surface of the dyeing vat body (1), a liquid storage tank (3) is provided inside the dyeing vat body (1), a water level monitoring mechanism (4) is provided on the right side of the upper surface of the dyeing vat body (1), a liquid inlet groove (5) is provided at the lower end of the water level monitoring mechanism (4), a cylinder (6) is provided inside the water level monitoring mechanism (4) for sliding, and a scale is provided on the rod surface of the water level monitoring mechanism (4) located on the outside, and a liquid outlet (7) is provided on the right side of the outer surface of the dyeing vat body (1).

2. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 1, characterized in that: A second connecting block (14) is fixedly provided on the left and right sides of the outer surface of the dyeing tank body (1), and a thread groove (15) is provided on the upper surface of the second connecting block (14).

3. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 1, characterized in that: A glass socket (8) is provided on the upper surface of the dyeing jar body (1), and a sealing gasket (9) is provided inside the glass socket (8).

4. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 3, characterized in that: A cover plate (10) is fixedly provided on the upper surface of the sealing gasket (9), connecting blocks (13) are fixedly provided on the left and right sides of the surface of the cover plate (10), and a lifting rod (11) is fixedly provided at the center of the upper surface of the cover plate (10).

5. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 4, characterized in that: The upper surface of the connecting block (13) is threadedly provided with a threaded rod (12), and the threaded rod (12) passes through the connecting block (13).

6. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 1, characterized in that: Universal wheels (20) are provided at the four corners of the lower surface of the dyeing vat body (1), and bolts (16) are fixedly provided on the left and right sides of the outer surface of the dyeing vat body (1), and the bolts (16) pass through a connecting plate (17).

7. The plastic staining jar for fluorescence in situ hybridization experiments according to claim 6, characterized in that: A connecting column (18) is fixedly provided at the upper end of one side of the opposite surface of the connecting plate (17), and a rubber gripping rod (19) is fixedly provided at the center of the outer surface of the connecting column (18).

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