Cell culture dish capable of simulating multiple environments

Abstract

The utility model relates to a cell culture dish capable of simulating multiple environments. Comprising a lower supporting structure arranged at the center, a plurality of culture bin lower bodies circumferentially distributed and connected to the periphery of the lower supporting structure, an upper supporting structure rotationally connected to the lower supporting structure, culture bin upper bodies circumferentially distributed and connected to the periphery of the upper supporting structure, and culture chambers connected to the lower portions of the culture bin upper bodies. The culture bin upper body covers the culture bin lower body, and the lower supporting structure and the upper supporting structure are movably connected up and down and are connected with a state locking structure. The culture dish provided by the utility model not only can simulate various cell culture environments, but also can quickly change cells into different environments for culture.

Description

Technical Field

[0001] The present application relates to the technical field of cell culture equipment, and in particular to a cell culture dish that simulates multiple environments. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. In the process of culturing biological cells, culture dishes are needed, and culture dishes are widely used in the process of biological cell culture.

[0003] The related technology discloses a cell culture dish structure, including a dish body, a diaphragm, a limiting cylinder, a fixed column, a support rod, a slider and a locking bolt. A limiting cylinder is fixed in the middle of the dish body, a fixed column is inserted in the limiting cylinder, and multiple diaphragms are evenly arranged on the sides of the limiting cylinder. The diaphragms divide the interior of the dish body into multiple spaces. A support rod is arranged in each space, one end of the support rod is connected to the fixed column, and the other end of the support rod is provided with a slide groove, a slider is slidably connected in the slide groove, a connecting rod is provided on the slider, and a culture chamber is provided at the end of the connecting rod.

[0004] With respect to the above-mentioned related technologies, the inventors found that the bacteria in each culture chamber can only be cultured in a single culture space, which has great limitations. When the cells change the culture environment, it is impossible to achieve rapid replacement. Utility Model Content

[0005] The purpose of this application is to provide a cell culture dish that simulates multiple environments, which can not only simulate a variety of cell culture environments, but also quickly change cells to different environments for culture.

[0006] The present application provides a cell culture dish simulating multiple environments using the following technical solutions:

[0007] A cell culture dish simulating multiple environments comprises a lower support structure arranged at the center, a plurality of culture bin lower bodies circumferentially distributed and connected to the lower support structure, an upper support structure rotatably connected to the lower support structure, a culture bin upper body circumferentially distributed and connected to the upper support structure, and a culture chamber connected below the culture bin upper body. The culture bin upper body covers the culture bin lower body, and the lower support structure and the upper support structure are movably connected up and down and are connected with a state locking structure.

[0008] As a preferred technical solution of the present application, the lower support structure includes a central tube with a sealed lower end, a central column coaxially fixed to the upper surface of the bottom plate of the central tube, and a support spring sleeved on the central column. The lower body of the culture chamber is evenly distributed circumferentially on the outer wall of the central tube, the upper support structure is inserted into the gap between the central tube and the central column, and the lower end of the upper support structure abuts against the upper end of the support spring.

[0009] As a preferred technical solution of the present application, the upper support structure includes a cannula with openings at both ends and a mounting plate fixed on the outer wall of the cannula. The lower end of the cannula is inserted into the gap between the central tube and the central column, and the upper body of the culture chamber is evenly distributed circumferentially on the lower surface of the mounting plate.

[0010] As a preferred technical solution of the present application, the state locking structure includes a threaded rod coaxially fixed to the upper end of the center column and a locking knob threadedly connected to the threaded rod. The threaded rod passes through the upper end of the tube, and the locking knob is connected to the protruding end of the threaded rod.

[0011] As a preferred technical solution of the present application, an outer clamping ring is coaxially fixed to the upper end of the central tube, and the inner wall of the outer clamping ring is evenly distributed with the same number of grooves as the lower body of the culture chamber. An inner clamping ring is fixed on the outer wall of the cannula below the mounting plate, and the outer wall of the inner clamping ring is evenly distributed with the same number of teeth as the grooves, and the teeth are engaged with the grooves.

[0012] As a preferred technical solution of the present application, the lower body of the culture chamber is configured as a cylindrical tube with an open upper end, and the outer wall is provided with a connecting arm connected to the central tube.

[0013] As a preferred technical solution of the present application, the upper body of the culture bin is configured as a cylindrical tube with the same diameter as the lower body of the culture bin, and a sealing gasket is provided on the lower end surface of the upper body of the culture bin.

[0014] As a preferred technical solution of the present application, the culture chamber includes a connecting rod connected to the inner wall of the upper body of the culture bin and a tray connected to the lower end of the connecting rod. The height of the tray is lower than the height of the lower end of the upper body of the culture bin, and the tray can be extended into the lower body of the culture bin.

[0015] In summary, this application includes at least one of the following beneficial technical effects:

[0016] 1. The culture chamber of the present application is provided with multiple components, and is composed of an upper body and a lower body. Through the relative rotation between the upper support structure and the lower support structure, the culture chamber on the upper body of the culture chamber can be driven to be replaced in a different culture chamber, so that cell culture can be carried out in different simulated environments.

[0017] 2. An inner clamping ring and an outer clamping ring are set between the upper supporting structure and the lower supporting structure. After the upper supporting structure and the lower supporting structure rotate relative to each other, the upper body and the lower body of the culture chamber can still be accurately aligned, eliminating the time for adjusting the alignment and realizing the rapid replacement of the cell culture environment.

[0018] 3. The setting of the support spring facilitates the rapid pop-up of the upper support structure, releasing the locked state with the lower support structure and facilitating the rotation of the upper support structure; the setting of the state locking structure facilitates the rapid closure of the culture chamber after the cell culture is changed to the simulated environment, reducing the impact of the external environment on the environment inside the culture chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 1 is a schematic diagram of the front view of the culture dish according to an embodiment of the present application;

[0020] Figure 2 Schematic diagram of the cross-sectional structure of the culture dish of the embodiment of the present application;

[0021] Figure 3 This is a schematic diagram of the top view of the lower support structure and the lower body of the culture chamber in the embodiment of the present application;

[0022] Figure 4 This is a bottom-up structural diagram of the upper support structure and the upper body of the culture chamber in an embodiment of the present application;

[0023] In the figure, 1. lower supporting structure; 11. center tube; 12. center column; 13. support spring; 14. outer clamping ring; 15. tooth groove; 2. lower body of culture chamber; 21. connecting arm; 3. upper supporting structure; 31. cannula; 32. mounting plate; 33. inner clamping ring; 34. latching teeth; 4. upper body of culture chamber; 41. sealing gasket; 5. culture chamber; 51. connecting rod; 52. tray; 6. state locking structure; 61. threaded rod; 62. locking knob. DETAILED DESCRIPTION

[0024] The following is combined with Figure 1 -Attached Figure 4 , further details of this application are given.

[0025] Example: This example proposes a cell culture dish that simulates multiple environments, referring to Figure 1-4The device includes a lower supporting structure 1, a culture bin lower body 2, an upper supporting structure 3, a culture bin upper body 4, a culture chamber 5 and a state locking structure 6. The lower support structure 1 serves as a supporting base and is placed on a working plane; there are several culture chamber lower bodies 2 evenly distributed and installed around the lower support structure 1. In this embodiment, four are used as an example for explanation. The culture chamber lower body 2 can simulate different cell culture environments by installing plug-ins and other structures; the upper support structure 3 and the lower support structure 1 can both rotate horizontally relative to each other and move up and down relative to each other to adjust the relative position; there are multiple culture chamber upper bodies 4 evenly distributed and installed around the upper support structure, and the number is the same as the number of culture chamber lower bodies 2. The culture chamber upper body 4 covers the culture chamber lower body 2 to form a closed cell culture environment; the culture chamber 5 is used to place cell embryos. The culture chamber 5 is installed inside the culture chamber upper body 4 and moves with the culture chamber upper body 4, following the culture chamber upper body 4 into different culture chamber lower bodies 2; the state locking structure 6 is connected to the lower support structure 1, and is used to lock the relative state between the lower support structure 1 and the upper support structure 3.

[0026] The lower support structure 1 includes a central tube 11, a central column 12, a support spring 13, and an outer collar 14. The central tube 11 is a cylindrical tube with a sealed lower end and an open upper end. The central column 12 is a cylinder, coaxially fixed at its lower end to the upper surface of the bottom plate of the central tube 11, with its upper end extending upward from the center tube 11. A cylindrical space is formed between the central tube 11 and the central column 12. The support spring 13 is placed in the cylindrical space and sleeved on the central column 12. The lower end of the support spring 13 is fixed to the bottom plate of the central tube 11. The outer collar 14 is coaxially fixed to the upper end of the central tube 11. The inner wall of the outer collar 14 is evenly distributed with the same number of tooth grooves 15 as the lower body 2 of the culture chamber. In this embodiment, four tooth grooves 15 are provided. The diameter of the outer collar 14 at its largest inner diameter is smaller than the inner diameter of the central tube 11.

[0027] The four culture bin lower bodies 2 are evenly distributed circumferentially on the outer wall of the central tube 11. The culture bin lower body 2 is configured as a cylindrical tube with an open upper end, and a bottom plate coplanar with the bottom surface of the central tube 11 is provided at the lower end. A connecting arm 21 is provided on the outer wall of the culture bin lower body 2, and the end of the connecting arm 21 is fixedly connected to the central tube 11, so that the central tube 11 and the four culture bin lower bodies 2 form a whole.

[0028] The lower portion of the upper support structure 3 is inserted into the cylindrical space between the central tube 11 and the central column 12. The upper support structure 3 includes an insert 31, a mounting plate 32, and an inner retaining ring 33. The insert 31 is configured as a circular tube with openings at both ends. Its outer diameter is smaller than the diameter of the smallest inner ring of the outer retaining ring 14, allowing the insert 31 to be inserted into the cylindrical space between the central tube 11 and the central column 12. The inner diameter of the insert 31 is not smaller than the diameter of the central column 12. The mounting plate 32 is configured as a circular disk and is fixed to the upper end of the outer wall of the insert 31. The inner retaining ring 33 is sleeved and fixed to the middle of the outer wall of the insert 31, at a position lower than the mounting plate 32. The axial length of the inner retaining ring 33 is greater than the axial length of the outer retaining ring 14. The outer wall of the inner retaining ring 33 is evenly distributed with the same number of teeth 34 as the number of teeth 15, which can mesh with the teeth 15.

[0029] The four culture bin upper bodies 4 are evenly distributed circumferentially and fixed on the lower surface of the mounting plate 32. The culture bin upper body 4 is set as a cylindrical tube with the same diameter as the culture bin lower body 2. The circumferential surface where the central axis of the four culture bin lower bodies 2 is located is the same as the circumferential surface where the central axis of the four culture bin upper bodies 4 is located, so that the culture bin upper body 4 can be directly covered on the culture bin lower body 2 up and down. In order to improve the sealing when covering, a sealing gasket 41 is fixed on the lower end surface of the culture bin upper body 4.

[0030] The culture chamber 5 is arranged in the space surrounded by the culture bin upper body 4. The culture chamber 5 includes a connecting rod 51 and a tray 52. ​​The tray 52 is coaxial with the culture bin upper body 4 and its height is lower than the height of the lower end of the culture bin upper body 4. The connecting rod 51 is parallel to the axis of the culture bin upper body 4. The upper end of the connecting rod 51 is fixedly connected to the inner wall of the culture bin upper body 4, and the lower end is connected to the outer peripheral surface of the tray 52. ​​When the culture bin upper body 4 covers the culture bin lower body 2, the tray 52 extends into the culture bin lower body 2.

[0031] In order to achieve the state locking between the lower support structure 1 and the upper support structure 3, a state locking structure 6 can be used. The state locking structure 6 includes a threaded rod 61 and a locking knob 62. The threaded rod 41 is coaxially fixed to the top of the center column 12 and has a diameter smaller than the diameter of the center column 12. The upper end of the threaded rod 61 can pass through the upper end of the insertion tube 31. The locking knob 62 is threadedly connected to the upper end of the threaded rod 61 and is used to abut against the upper end surface of the insertion tube 31 to lock the state of the upper support structure 3. At this time, the support spring 13 is in a compressed state.

[0032] The implementation principle of the embodiment of the present application is as follows: when culturing cells, the cell embryos are placed in the tray 52 of each culture chamber 5, the upper support structure 3 is pressed down, and the latch teeth 34 on the inner clamping ring 33 are aligned with the tooth grooves 15 on the outer clamping ring 14 below, the lower end of the cannula 31 is gradually inserted into the space between the central tube 11 and the central column 12 and the support spring 13 is squeezed until the upper body 4 of the culture chamber covers the lower body 2 of the culture chamber, at which time the latch teeth 34 are stuck in the tooth grooves 15, and then the locking knob 62 is tightened on the threaded rod 61; when changing the culture environment of the cell embryos, the locking knob 62 is turned upward to loosen the upper support structure 3 and, under the elastic force of the support spring 13, moves upward until the latch teeth 34 are disengaged from the tooth grooves 15, and then one or more tooth positions of the latch teeth 34 are rotated, and then the upper support structure 3 is pressed down again, so that the latch teeth 34 are re-stuck in the tooth grooves 15 and the upper body 4 of the culture chamber covers the lower body 2 of the culture chamber again, and then the locking knob 62 is tightened again.

[0033] The examples of this specific embodiment are all preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, any equivalent changes made based on the structure, shape, and principle of this application should be included in the scope of protection of this application.

Claims

1. A cell culture dish simulating multiple environments, characterized in that: The invention comprises a lower support structure (1) arranged at the center, a plurality of culture chamber lower bodies (2) circumferentially distributed and connected to the lower support structure (1), an upper support structure (3) rotatably connected to the lower support structure (1), a culture chamber upper body (4) circumferentially distributed and connected to the upper support structure (3), and a culture chamber (5) connected below the culture chamber upper body (4); the culture chamber upper body (4) covers the culture chamber lower body (2); the lower support structure (1) and the upper support structure (3) are movably connected up and down and are connected with a state locking structure (6).

2. The cell culture dish simulating multiple environments according to claim 1, characterized in that: The lower support structure (1) comprises a central tube (11) with a sealed lower end, a central column (12) coaxially fixed to the upper surface of the bottom plate of the central tube (11), and a support spring (13) sleeved on the central column (12); the lower body (2) of the culture chamber is uniformly distributed on the outer wall of the central tube (11) in a circumferential direction; the upper support structure (3) is inserted into the gap between the central tube (11) and the central column (12); and the lower end of the upper support structure (3) abuts against the upper end of the support spring (13).

3. The cell culture dish simulating multiple environments according to claim 2, characterized in that: The upper support structure (3) includes an intubation tube (31) with openings at both ends and a mounting plate (32) fixed to the outer wall of the intubation tube (31). The lower end of the intubation tube (31) is inserted into the gap between the central tube (11) and the central column (12). The upper body (4) of the culture chamber is evenly distributed circumferentially on the lower surface of the mounting plate (32).

4. The cell culture dish simulating multiple environments according to claim 3, characterized in that: The state locking structure (6) comprises a threaded rod (61) coaxially fixed to the upper end of the central column (12) and a locking knob (62) threadedly connected to the threaded rod (61); the threaded rod (61) passes through the upper end of the cannula (31), and the locking knob (62) is connected to the protruding end of the threaded rod (61).

5. The cell culture dish simulating multiple environments according to claim 3, characterized in that: An outer clamping ring (14) is coaxially fixed to the upper end of the central tube (11), and the inner wall of the outer clamping ring (14) is evenly distributed with tooth grooves (15) of the same number as the lower body (2) of the culture chamber. An inner clamping ring (33) is fixed to the outer wall of the cannula (31) below the mounting plate (32), and the outer wall of the inner clamping ring (33) is evenly distributed with latch teeth (34) of the same number as the tooth grooves (15), and the latch teeth (34) are meshed with the tooth grooves (15).

6. The cell culture dish simulating multiple environments according to claim 1, characterized in that: The lower body (2) of the culture chamber is configured as a cylindrical tube with an open upper end, and a connecting arm (21) connected to the central tube (11) is provided on the outer wall.

7. The cell culture dish simulating multiple environments according to claim 6, characterized in that: The culture bin upper body (4) is configured as a cylindrical tube having the same diameter as the culture bin lower body (2), and a sealing gasket (41) is provided on the lower end surface of the culture bin upper body (4).

8. The cell culture dish simulating multiple environments according to claim 7, characterized in that: The culture chamber (5) comprises a connecting rod (51) connected to the inner wall of the culture chamber upper body (4) and a tray (52) connected to the lower end of the connecting rod (51). The height of the tray (52) is lower than the height of the lower end of the culture chamber upper body (4). The tray (52) can extend into the culture chamber lower body (2).

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