Circulating tumor cell detection device

Abstract

The utility model discloses a circulating tumor cell detection device which comprises a bottom plate and a rotating shaft rotatably inserted on the bottom plate, the upper end of the rotating shaft is fixedly connected with a rotating disc, the top of the rotating disc is provided with a plurality of grooves which are circumferentially distributed, the top of the bottom plate is fixedly connected with a support, the upper end of the support is fixedly connected with a transversely arranged liquid storage box, and the liquid storage box is fixedly connected with the rotating disc. A partition plate is fixedly connected between the inner walls of the liquid storage box, a first one-way valve is fixedly installed on the partition plate, and a second one-way valve is fixedly installed at the bottom of the liquid storage box. According to the device, a sample containing blood is placed in the culture dish, then the culture dish is placed in the groove, then the rotating disc is driven to rotate, when the infrared receiver corresponds to the infrared transmitter in position, the controller sends a signal to the air cylinder, the air cylinder pushes the push plate to move, and calibration liquid is discharged through the second one-way valve and drips into the culture dish. The purpose of automatic titration is achieved, and a plurality of samples can be continuously calibrated.

Description

Technical Field

[0001] The utility model relates to the technical field of cell detection, in particular to a circulating tumor cell detection device. Background Art

[0002] Malignant tumors will spread to other organs of the body through the blood, and tumor metastasis is the main cause of death in cancer patients. Tumor cells invade the surrounding tissues of primary tumor cells, enter the blood and lymphatic system, form circulating tumor cells (circulating tumor cells are abbreviated as CTCs, and tumor cells that enter the human peripheral blood are usually called circulating tumor cells), and are transported to distal tissues, then exudate, adapt to the new microenvironment, and eventually "seed", "proliferate", and "colonize" to form metastatic lesions. Therefore, early detection of CTCs in the blood plays an important guiding role in patient prognosis, efficacy evaluation and personalized treatment. Testing the patient's blood through a detection device is an effective means to detect tumor cells in a timely manner, thereby facilitating subsequent treatment based on the test results.

[0003] In the process of detecting circulating tumor cells, circulating tumor cells need to be labeled by using specific antibodies to bind to antigens on the surface of tumor cells, thereby marking the tumor cells. The existing technology requires manual labeling of circulating tumor cells, and it is impossible to label multiple samples continuously and quickly, so it is urgent to solve this problem. Utility Model Content

[0004] (1) Technical problems solved

[0005] In view of the deficiencies in the prior art, the present invention provides a circulating tumor cell detection device that solves the problems raised in the above-mentioned background technology.

[0006] (2) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a circulating tumor cell detection device, comprising a base plate, a rotating shaft rotatably inserted on the base plate, the upper end of the rotating shaft is fixedly connected to a turntable, the top of the turntable is provided with a plurality of circumferentially distributed grooves, the top of the base plate is fixedly connected to a bracket, the upper end of the bracket is fixedly connected to a horizontally placed liquid storage box, a partition is fixedly connected between the inner walls of the liquid storage box, a first one-way valve is fixedly installed on the partition, a second one-way valve is fixedly installed on the bottom of the liquid storage box, the partition divides the liquid storage box into an upper cavity and a lower cavity, a push plate is provided inside the lower cavity, a cylinder is fixedly installed at the end of the liquid storage box, the output end of the cylinder passes through the interior of the lower cavity and is fixedly connected to the push plate.

[0008] Preferably, a liquid inlet pipe is fixedly connected to the top of the liquid storage box, and a sealing plug is inserted into the liquid inlet pipe.

[0009] Preferably, an infrared receiver is fixedly mounted on the circumferential side wall of the turntable, an infrared transmitter is fixedly mounted on the side wall of the bracket, and a controller is fixedly mounted on the top of the base plate.

[0010] Preferably, the lower end of the rotating shaft is fixedly connected to a driven gear, a motor is fixedly installed on the bottom of the base plate, an output end of the motor is fixedly connected to a driving gear, and the driving gear is meshed with the driven gear.

[0011] Preferably, the push plate is a rectangular plate, and the push plate is slidably connected to the inner wall of the lower cavity.

[0012] Preferably, the groove is a circular groove.

[0013] (3) Beneficial effects

[0014] The utility model provides a circulating tumor cell detection device, which has the following beneficial effects:

[0015] 1. In the present invention, a blood sample is placed in a culture dish, which is then placed in a groove. The turntable is then driven to rotate. When the infrared receiver and the infrared transmitter are positioned correspondingly, the controller sends a signal to the cylinder, which pushes the push plate to move, discharging the calibration liquid through the second one-way valve and dripping it into the culture dish, thereby achieving the purpose of automatic titration. Multiple samples can be calibrated continuously.

[0016] 2. In the present invention, calibration liquid is added to the upper cavity inside the liquid storage box through the liquid inlet pipe. When the push plate moves away from the second one-way valve, the calibration liquid inside the upper cavity is sucked into the lower cavity through the first one-way valve, thereby achieving the purpose of liquid addition; when the push plate moves close to the second one-way valve, the calibration liquid is discharged through the second one-way valve. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a front perspective structural diagram of a circulating tumor cell detection device proposed in the present invention;

[0018] Figure 2 This is a cross-sectional structural diagram of a circulating tumor cell detection device proposed in the present invention;

[0019] Figure 3 This is a bottom-up stereoscopic structural diagram of a circulating tumor cell detection device proposed in the present invention;

[0020] Figure 4 for Figure 2 A magnified structure diagram.

[0021] In the figure: 1. bottom plate; 2. turntable; 201. groove; 3. driven gear; 4. rotating shaft; 5. motor; 6. driving gear; 7. liquid storage box; 8. partition; 9. first one-way valve; 10. second one-way valve; 11. push plate; 12. cylinder; 13. infrared transmitter; 14. controller; 15. infrared receiver; 16. bracket; 17. liquid inlet pipe. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0023] See also Figures 1 to 4 The utility model provides a technical solution: a circulating tumor cell detection device, comprising a base plate 1, a rotating shaft 4 rotatably inserted on the base plate 1, a turntable 2 fixedly connected to the upper end of the rotating shaft 4, a plurality of circumferentially distributed grooves 201 are provided on the top of the turntable 2, the grooves 201 are circular grooves, a bracket 16 is fixedly connected to the top of the base plate 1, a horizontal liquid storage box 7 is fixedly connected to the upper end of the bracket 16, a partition 8 is fixedly connected between the inner walls of the liquid storage box 7, a first one-way valve 9 is fixedly installed on the partition 8, a second one-way valve 10 is fixedly installed at the bottom of the liquid storage box 7, the partition 8 divides the liquid storage box 7 into an upper cavity and a lower cavity, a push plate 11 is provided inside the lower cavity, a cylinder 12 is fixedly installed at the end of the liquid storage box 7, and the output end of the cylinder 12 passes through the interior of the lower cavity and is fixedly connected to the push plate 11.

[0024] An infrared receiver 15 is fixedly mounted on the circumferential side wall of the turntable 2 , an infrared transmitter 13 is fixedly mounted on the side wall of the bracket 16 , and a controller 14 is fixedly mounted on the top of the base plate 1 . The controller 14 is electrically connected to the infrared receiver 15 and the cylinder 12 .

[0025] Place the blood sample in a culture dish, then place the culture dish in the groove 201, and then drive the turntable 2 to rotate. When the infrared receiver 15 corresponds to the position of the infrared transmitter 13, the infrared transmitter 13 transmits a signal to the controller 14. At this time, the controller 14 sends a signal to the cylinder 12. The cylinder 12 pushes the push plate 11 to move, and the calibration liquid is discharged through the second one-way valve 10 and drips into the culture dish, thereby achieving the purpose of automatic titration. In addition, multiple samples can be calibrated continuously.

[0026] Furthermore, a liquid inlet pipe 17 is fixedly connected to the top of the liquid storage box 7, and a sealing plug is inserted inside the liquid inlet pipe 17. Calibration liquid is added to the upper cavity inside the liquid storage box 7 through the liquid inlet pipe 17. When the push plate 11 moves away from the second one-way valve 10, the calibration liquid inside the upper cavity is sucked into the lower cavity through the first one-way valve 9, thereby achieving the purpose of liquid addition; when the push plate 11 moves close to the second one-way valve 10, the calibration liquid is discharged through the second one-way valve 10.

[0027] The lower end of the rotating shaft 4 is fixedly connected to the driven gear 3, the bottom of the base plate 1 is fixedly installed with a motor 5, the output end of the motor 5 is fixedly connected to the driving gear 6, and the driving gear 6 is meshed with the driven gear 3.

[0028] By starting the motor 5 , the driving gear 6 is driven to rotate, the driving gear 6 drives the driven gear 3 to rotate, and the driven gear 3 drives the turntable 2 to rotate through the rotating shaft 4, thereby transporting each culture dish to the calibration position, that is, directly below the second one-way valve 10.

[0029] The push plate 11 is a rectangular plate, which is slidably connected to the inner wall of the lower cavity, and a sealing ring can be provided on the push plate 11 to ensure the sealing between the push plate 11 and the lower cavity and prevent water seepage.

[0030] The above shows and describes the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely preferred examples of the present invention and are not intended to limit the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and improvements fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A circulating tumor cell detection device, characterized in that: The invention comprises a base plate (1), a rotating shaft (4) rotatably inserted on the base plate (1), the upper end of the rotating shaft (4) is fixedly connected to a turntable (2), the top of the turntable (2) is provided with a plurality of grooves (201) distributed circumferentially, the top of the base plate (1) is fixedly connected to a bracket (16), the upper end of the bracket (16) is fixedly connected to a horizontally placed liquid storage box (7), a partition (8) is fixedly connected between the inner walls of the liquid storage box (7), a first one-way valve (9) is fixedly installed on the partition (8), a second one-way valve (10) is fixedly installed at the bottom of the liquid storage box (7), the partition (8) divides the liquid storage box (7) into an upper cavity and a lower cavity, a push plate (11) is provided inside the lower cavity, a cylinder (12) is fixedly installed at the end of the liquid storage box (7), the output end of the cylinder (12) passes through the interior of the lower cavity and is fixedly connected to the push plate (11).

2. A circulating tumor cell detection device according to claim 1, characterized in that: A liquid inlet pipe (17) is fixedly connected to the top of the liquid storage box (7), and a sealing plug is inserted into the liquid inlet pipe (17).

3. The circulating tumor cell detection device according to claim 1, characterized in that: An infrared receiver (15) is fixedly mounted on the circumferential side wall of the turntable (2), an infrared transmitter (13) is fixedly mounted on the side wall of the bracket (16), and a controller (14) is fixedly mounted on the top of the base plate (1).

4. The circulating tumor cell detection device according to claim 1, characterized in that: The lower end of the rotating shaft (4) is fixedly connected to a driven gear (3), the bottom of the base plate (1) is fixedly mounted with a motor (5), the output end of the motor (5) is fixedly connected to a driving gear (6), and the driving gear (6) is meshed with the driven gear (3).

5. The circulating tumor cell detection device according to claim 1, characterized in that: The push plate (11) is a rectangular plate, and the push plate (11) is slidably connected to the inner wall of the lower cavity.

6. The circulating tumor cell detection device according to claim 1, characterized in that: The groove (201) is a circular groove.

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