Multi-channel immunoblotting membrane washing platform

Abstract

The utility model discloses a multichannel immunoblotting membrane washing platform which comprises a bottom plate, the top of the bottom plate is fixedly connected with first springs, the tops of the first springs are jointly and fixedly connected with a membrane washing box, the front portion of the membrane washing box is provided with a collecting assembly, the rear portion of the membrane washing box is provided with a conveying assembly, and the top of the bottom plate is provided with a shaking assembly. The shaking assembly is located on the left side of the film washing box. By designing the shaking assembly, the film washing box can swing at a stable frequency, so that the film washing effect is improved, the control handle can be lifted upwards in the equipment operation process to enable the pressing block to press the smooth surface of the rack, the rack and the gear are meshed with each other, the rack can drive the gear to rotate at a fixed frequency, and the film washing effect is improved. Therefore, the effect that the film washing box swings at a stable frequency is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein blot processing devices, in particular to a multi-channel immunoblotting membrane washing platform. Background Art

[0002] Protein immunoblotting is a hybridization technique that combines high-resolution gel electrophoresis and immunochemical analysis techniques. It has the advantages of large analytical capacity, high sensitivity, and strong specificity. It is the most commonly used method for detecting protein characteristics, expression, and distribution.

[0003] Chinese patent publication number CN214427451 U discloses a fully automated protein blotting processing and detection device, including an infusion pipeline, a pipetting mechanism, and an incubation box. The infusion pipeline is equipped with a switching valve and a peristaltic pump. The above document converts the multi-channel infusion pipeline into a single input channel by controlling the switching valve, and completes the actual addition and removal during the protein immunoblotting experiment through the pipetting mechanism. This fully automates the blocking, membrane washing, antibody incubation, and development and photography processes in the protein immunoblotting experiment after transfer, saving time and labor and ensuring the accuracy of the experimental results. In addition, antibodies can be automatically recovered, saving the amount of reagents.

[0004] The above patent document can realize the protein immunoblotting membrane washing operation, but the frequency of the oscillation is not stable enough, and the operation process cannot be stopped or adjusted during the operation, thereby affecting the work efficiency of the staff. Utility Model Content

[0005] The main purpose of the utility model is to provide a multi-channel immunoblotting membrane washing platform, which can effectively solve the problems of unstable oscillation frequency and inability to stop or adjust the operation process during operation.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A multi-channel immunoblotting membrane washing platform comprises a base plate, a plurality of springs are fixedly connected to the top of the base plate, a membrane washing box is connected to the top of each spring, a collection component is provided at the front of the membrane washing box, a conveying component is provided at the rear of the membrane washing box, a shaking component is provided at the top of the base plate, and the shaking component is located on the left side of the membrane washing box.

[0008] Preferably, the collecting component includes a waste liquid box, the bottom of the waste liquid box is fixedly connected to the top of the base plate, and a number of evenly distributed liquid pumps are fixedly installed on the top of the waste liquid box. The input end of the liquid pump is fixedly connected to a connecting pipe, and the other end of the connecting pipe passes through the front end of the film washing box and extends to the bottom wall of the film washing box.

[0009] Preferably, the conveying assembly includes a support frame, the bottom of the support frame is fixedly connected to the top of the base plate, a branch pipe is fixedly connected to the top of the support frame, and the front end of the branch pipe is fixedly connected to the rear end of the membrane washing box, a peristaltic pump and a liquid storage box are fixedly installed on the rear side of the top of the base plate, and the output end of the peristaltic pump is fixedly connected to the rear end of the branch pipe, and a connecting pipe is fixedly connected between the peristaltic pump and the liquid storage box.

[0010] Preferably, the shaking assembly includes a fixed block, the bottom of the fixed block is fixedly connected to the top of the base plate, the left end of the fixed block is fixedly connected to an L-shaped plate, the left end of the fixed block is provided with a control component, the left end of the fixed block is fixedly connected to a mounting support, the top of the mounting support is fixedly installed with a motor, and the left end of the fixed block is provided with a rotating component.

[0011] Preferably, the control component includes a limit column 1 and a limit column 2, and the limit column 1 and the limit column 2 are both fixedly connected to the left end of the fixed block, and the left end of the fixed block is fixedly connected to the L-shaped plate 2, and a control handle is rotatably connected between the fixed block and the L-shaped plate 2, and the left end of the control handle is rotatably connected to the movable rod, and the left end of the fixed block is fixedly connected to the fixed column 1, and the outer surface of the fixed column 1 is rotatably connected to the pressing block, and the left end of the pressing block is rotatably connected to the right end of the movable rod.

[0012] Preferably, the rotating component includes a rotating shaft 1, one end of the rotating shaft 1 is fixedly connected to the output end of the motor through a coupling, the other end of the rotating shaft 1 passes through the L-shaped plate 1 and is fixedly connected to a crank, the end of the crank away from the motor is rotatably connected to a rack, the left end of the fixed block is fixedly connected to a fixed column 2, the left end of the rack is fixedly connected to a fixed column 3, a spring 2 is fixedly connected between the fixed column 2 and the fixed column 3, the left end of the film washing box is fixedly connected to the rotating shaft 2, and the left end of the rotating shaft 2 is rotatably connected to the left side wall of the L-shaped plate 1, and the outer surface of the rotating shaft 2 is fixedly connected to a gear.

[0013] Preferably, a plurality of detachable partitions are provided inside the film washing box, a baffle is fixedly connected to the top of the bottom plate, and the baffle is located on the right side of the film washing box.

[0014] Preferably, all the pipe bodies of the connecting pipe and the branch pipe are hoses.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The shaking assembly in the utility model can make the film washing box swing at a stable frequency, thereby improving the film washing effect. During the operation of the equipment, the control handle can be lifted upward to make the pressing block press the smooth surface of the rack, so that the rack and the gear are engaged with each other, so that the rack can drive the gear to rotate at a fixed frequency, thereby achieving the effect of the film washing box swinging at a stable frequency.

[0017] At the same time, during the operation, you only need to pull the control handle downward to release the pressure of the pressing block on the rack, so that the rack disengages from the gear and thus suspends the swing operation of the membrane washing box, improving the controllability of the experiment and thereby improving the work efficiency of the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall structure of the multi-channel immunoblotting membrane washing platform of the utility model;

[0019] Figure 2 This is a schematic diagram of the overall structure of the multi-channel immunoblotting membrane washing platform of the utility model from another perspective;

[0020] Figure 3 This is a schematic diagram of the partial structure of the multi-channel immunoblotting membrane washing platform of the utility model. Figure 1 ;

[0021] Figure 4 This is a schematic diagram of the partial structure of the multi-channel immunoblotting membrane washing platform of the utility model. Figure 2 ;

[0022] Figure 5 For the utility model Figure 1 A in the middle is an enlarged schematic diagram;

[0023] Figure 6 For the utility model Figure 1 The enlarged schematic diagram of point B in the middle;

[0024] Figure 7 For the utility model Figure 3 Enlarged schematic diagram at point C in the middle;

[0025] Figure 8 For the utility model Figure 4 Enlarged schematic diagram at point D in the middle.

[0026] In the figure: 1. bottom plate; 2. spring 1; 3. membrane washing box; 4. collecting component; 41. waste liquid box; 42. liquid pump; 43. connecting pipe; 5. conveying component; 51. support frame; 52. branch pipe; 53. peristaltic pump; 54. connecting pipe; 55. liquid storage box; 6. shaking component; 61. fixed block; 62. L-shaped plate 1; 63. control component; 631. limit column 1; 632. limit column 2; 633. L-shaped plate 2; 634. control handle; 635. movable rod; 636. fixed column 1; 637. pressing block; 64. mounting support; 65. motor; 66. rotating component; 661. rotating shaft 1; 662. crank; 663. rack; 664. fixed column 2; 665. fixed column 3; 666. spring 2; 667. rotating shaft 2; 668. gear. DETAILED DESCRIPTION

[0027] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0028] Example 1:

[0029] like Figure 1 As shown, this embodiment provides a multi-channel immunoblotting membrane washing platform, including a bottom plate 1, a plurality of springs 2 (for example, there may be four springs 2 in this embodiment) are fixedly connected to the top of the bottom plate 1, and a membrane washing box 3 is fixedly connected to the top of each spring 2, and a plurality of detachable partitions are provided inside the membrane washing box 3. A baffle is fixedly connected to the top of the bottom plate 1, and the baffle is located on the right side of the membrane washing box 3;

[0030] The detachable partition can be replaced according to actual conditions to facilitate cleaning operations by the staff. The function of the baffle is mainly to limit the movement direction of the film washing box 3 to ensure the normal operation of the equipment.

[0031] like Figure 2 and Figure 3 As shown, a collecting assembly 4 is provided at the front of the film washing box 3, and the collecting assembly 4 includes a waste liquid box 41. The bottom of the waste liquid box 41 is fixedly connected to the top of the base plate 1, and a plurality of evenly distributed liquid suction pumps 42 are fixedly installed on the top of the waste liquid box 41. The input end of the liquid suction pump 42 is fixedly connected to a connecting pipe 43, and the other end of the connecting pipe 43 passes through the front end of the film washing box 3 and extends to the bottom wall of the film washing box 3. When the film washing operation is completed, the used film washing liquid inside the film washing box 3 can be extracted by the liquid suction pump 42 through the connecting pipe 43 and input into the waste liquid box 41 for collection;

[0032] like Figure 2 and Figure 3 As shown, a conveying assembly 5 is provided at the rear of the film washing box 3, and the conveying assembly 5 includes a support frame 51, the bottom of the support frame 51 is fixedly connected to the top of the base plate 1, a branch pipe 52 is fixedly connected to the top of the support frame 51, and the front end of the branch pipe 52 is fixedly connected to the rear end of the film washing box 3, a peristaltic pump 53 and a liquid storage box 55 are fixedly installed on the rear side of the top of the base plate 1, and the output end of the peristaltic pump 53 is fixedly connected to the rear end of the branch pipe 52, and a connecting pipe 54 is fixedly connected between the peristaltic pump 53 and the liquid storage box 55, and all the pipe bodies of the connecting pipe 43 and the branch pipe 52 are hoses. The length of this part of the hose can be designed according to actual conditions as long as it does not affect the operation of the equipment;

[0033] The peristaltic pump 53 used in this solution is a conventional design of the prior art, which is mainly composed of a driver, a pump head and a pump tube. It has a good self-priming function, can prevent backflow, and facilitates the transportation of fluids;

[0034] Before washing the membrane, it is necessary to open the peristaltic pump 53 to extract the membrane washing liquid inside the liquid storage box 55 through the connecting pipe 54 and transport it to the compartment inside the membrane washing box 3 through the branch pipe 52 to facilitate subsequent membrane washing operations. A valve is also provided at the connection between the membrane washing box 3 and the branch pipe 52, and the staff can adjust it according to actual conditions.

[0035] like Figure 2 、 Figure 3 and Figure 4 As shown, a shaking assembly 6 is provided on the top of the base plate 1, and the shaking assembly 6 is located on the left side of the film washing box 3. The shaking assembly 6 includes a fixed block 61. The bottom of the fixed block 61 is fixedly connected to the top of the base plate 1. The left end of the fixed block 61 is fixedly connected to an L-shaped plate 62. The left end of the fixed block 61 is provided with a control component 63. The left end of the fixed block 61 is fixedly connected to a mounting support 64. A motor 65 is fixedly installed on the top of the mounting support 64. The left end of the fixed block 61 is provided with a rotating component 66. During the operation of the equipment, the control component 63, the motor 65 and the rotating component 66 cooperate with each other to ensure that the equipment can operate stably, thereby performing a smooth swing operation on the film washing box 3.

[0036] like Figure 5 and Figure 6 As shown, the control component 63 includes a limiting column 1 631 and a limiting column 2 632, and the limiting column 1 631 and the limiting column 2 632 are both fixedly connected to the left end of the fixed block 61, and the left end of the fixed block 61 is fixedly connected to the L-shaped plate 2 633. A control handle 634 is rotatably connected between the fixed block 61 and the L-shaped plate 2 633, and the left end of the control handle 634 is rotatably connected to the movable rod 635. The left end of the fixed block 61 is fixedly connected to a fixing column 1 636, and the outer surface of the fixing column 1 636 is rotatably connected to a pressing block 637, and the left end of the pressing block 637 is rotatably connected to the right end of the movable rod 635.

[0037] When the swinging operation of the device needs to be stopped during operation, the control handle 634 needs to be pulled downward, thereby driving the movable rod 635 to rotate, and then driving the pressing block 637 to rotate with the axis of the fixed column 636 as the reference, so that the pressing block 637 releases the pressure on the rack 663. At this time, the rack 663 is disengaged from the gear 668, thereby stopping the swinging operation.

[0038] Example 2:

[0039] The only difference between this embodiment and embodiment 1 is that Figure 6 、 Figure 7 and Figure 8As shown, the rotating component 66 includes a rotating shaft 1 661, one end of the rotating shaft 1 661 is fixedly connected to the output end of the motor 65 through a coupling, the other end of the rotating shaft 1 661 passes through the L-shaped plate 1 62 and is fixedly connected to a crank 662, and the end of the crank 662 away from the motor 65 is rotatably connected to a rack 663, the left end of the fixed block 61 is fixedly connected to a fixed column 2 664, and the left end of the rack 663 is fixedly connected to a fixed column 3 665, and a spring 2 666 is fixedly connected between the fixed column 2 664 and the fixed column 3 665. The spring 2 666 mainly ensures that the rack 663 will not separate from the equipment, thereby improving the stability of the equipment operation. The left end of the film washing box 3 is fixedly connected to the rotating shaft 2 667, and the left end of the rotating shaft 2 667 is rotatably connected to the left side wall of the L-shaped plate 1 62, and the outer surface of the rotating shaft 2 667 is fixedly connected to a gear 668;

[0040] When the membrane washing liquid fills the bottom of the membrane washing box 3, start the motor 65, and the motor 65 drives the rotating shaft 1 661 to rotate through the coupling, thereby further driving the crank 662 to rotate. When the crank 662 rotates, it drives the rack 663 to rotate. At this time, pull up the control handle 634. At this time, the control handle 634 drives the movable rod 635 to rotate, thereby driving the fixed column 1 636 to press the rack 663. The smooth surface of the rack 663 can slide on the arc surface of the pressing block 637. At this time, the rack 663 and the gear 668 engage with each other to rotate back and forth at a fixed frequency, thereby driving the rotating shaft 2 667 to rotate, and thus realizing that the membrane washing box 3 can swing at a fixed frequency.

[0041] The working principle of the present invention is as follows: first, the peristaltic pump 53 is started, and the peristaltic pump 53 draws out the membrane washing liquid inside the liquid storage box 55 through the connecting pipe 54 and transports it to the partition inside the membrane washing box 3 through the branch pipe 52, and then the motor 65 is started. The motor 65 drives the rotating shaft 1 661 to rotate through the coupling, thereby further driving the crank 662 to rotate. When the crank 662 rotates, it drives the rack 663 to rotate. At this time, the control handle 634 is pulled up. At this time, the control handle 634 drives the movable rod 635 to rotate, thereby driving the fixed column 1 636 to press the rack 663. The smooth surface of the rack 663 can slide on the arc surface of the pressing block 637. At this time, the rack 663 and gear 668 mesh with each other and rotate back and forth at a fixed frequency, thereby driving the rotating shaft 2 667 to rotate, and then driving the membrane washing box 3 to swing; when the swinging operation of the equipment needs to be stopped during operation, it is necessary to pull down the control handle 634, thereby driving the movable rod 635 to rotate, and then driving the pressing block 637 to rotate with the axis of the fixed column 1 636 as the reference, so that the pressing block 637 releases the pressure on the rack 663, and then releases the meshing effect between the rack 663 and the gear 668; at the end of the membrane washing operation, the used membrane washing liquid inside the membrane washing box 3 can be extracted by the liquid pump 42 through the connecting pipe 43, and input into the waste liquid box 41 for collection.

[0042] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A multi-channel immunoblotting membrane washing platform, comprising a bottom plate (1), characterized in that: A plurality of springs (2) are fixedly connected to the top of the bottom plate (1), and the top of each spring (2) is fixedly connected to a film washing box (3). A collecting assembly (4) is provided at the front of the film washing box (3), and a conveying assembly (5) is provided at the rear of the film washing box (3). A shaking assembly (6) is provided at the top of the bottom plate (1), and the shaking assembly (6) is located on the left side of the film washing box (3).

2. The multi-channel immunoblotting membrane washing platform according to claim 1, characterized in that: The collecting assembly (4) comprises a waste liquid box (41), the bottom of the waste liquid box (41) being fixedly connected to the top of the base plate (1).

3. The multi-channel immunoblotting membrane washing platform according to claim 2, characterized in that: A liquid extraction pump (42) is fixedly installed on the top of the waste liquid box (41), and a connecting pipe (43) is fixedly connected to the input end of the liquid extraction pump (42), and the other end of the connecting pipe (43) passes through the front end of the membrane washing box (3) and extends to the bottom wall of the membrane washing box (3).

4. The multi-channel immunoblotting membrane washing platform according to claim 3, characterized in that: The conveying assembly (5) comprises a support frame (51), the bottom of the support frame (51) is fixedly connected to the top of the base plate (1), the top of the support frame (51) is fixedly connected to a branch pipe (52), and the front end of the branch pipe (52) is fixedly connected to the rear end of the film washing box (3).

5. The multi-channel immunoblotting membrane washing platform according to claim 4, characterized in that: A peristaltic pump (53) and a liquid storage box (55) are fixedly installed on the rear side of the top of the base plate (1), and the output end of the peristaltic pump (53) is fixedly connected to the rear end of the branch pipe (52), and a connecting pipe (54) is fixedly connected between the peristaltic pump (53) and the liquid storage box (55).

6. The multi-channel immunoblotting membrane washing platform according to claim 1, characterized in that: The shaking assembly (6) comprises a fixed block (61), the bottom of the fixed block (61) is fixedly connected to the top of the base plate (1), the left end of the fixed block (61) is fixedly connected to an L-shaped plate (62), the left end of the fixed block (61) is provided with a control component (63), the left end of the fixed block (61) is fixedly connected to a mounting support (64), the top of the mounting support (64) is fixedly installed with a motor (65), and the left end of the fixed block (61) is provided with a rotating component (66).

7. The multi-channel immunoblotting membrane washing platform according to claim 6, characterized in that: The control component (63) includes a limiting column 1 (631) and a limiting column 2 (632), wherein the limiting column 1 (631) and the limiting column 2 (632) are both fixedly connected to the left end of the fixed block (61), the left end of the fixed block (61) is fixedly connected to the L-shaped plate 2 (633), a control handle (634) is rotatably connected between the fixed block (61) and the L-shaped plate 2 (633), the left end of the control handle (634) is rotatably connected to the movable rod (635), the left end of the fixed block (61) is fixedly connected to the fixing column 1 (636), the outer surface of the fixing column 1 (636) is rotatably connected to the pressing block (637), and the left end of the pressing block (637) is rotatably connected to the right end of the movable rod (635).

8. The multi-channel immunoblotting membrane washing platform according to claim 7, characterized in that: The rotating component (66) includes a rotating shaft (661), one end of which is fixedly connected to the output end of the motor (65) through a coupling, and the other end of the rotating shaft (661) passes through the L-shaped plate (62) and is fixedly connected to a crank (662), and the end of the crank (662) away from the motor (65) is rotatably connected to a rack (663), the left end of the fixed block (61) is fixedly connected to a fixed column (664), the left end of the rack (663) is fixedly connected to a fixed column (665), and a spring (666) is fixedly connected between the fixed column (664) and the fixed column (665), the left end of the film washing box (3) is fixedly connected to the rotating shaft (667), and the left end of the rotating shaft (667) is rotatably connected to the left side wall of the L-shaped plate (62), and the outer surface of the rotating shaft (667) is fixedly connected to a gear (668).

9. The multi-channel immunoblotting membrane washing platform according to claim 1, characterized in that: A plurality of detachable partitions are provided inside the film washing box (3), and a baffle is fixedly connected to the top of the bottom plate (1), and the baffle is located on the right side of the film washing box (3).

10. The multi-channel immunoblotting membrane washing platform according to claim 4, characterized in that: All the pipe bodies of the connecting pipe (43) and the branch pipe (52) are hoses.

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