Disposable six-hole cell culture plate for cell slide
By introducing the design of slide trough and slide clamping ears in the six-well cell culture plate, the problems of cover glass being difficult to remove due to adsorption and easy to be crushed by clamping are solved, and the non-destructive and convenient operation of cell slide climbing is realized, ensuring the smooth progress of the experiment.
Patent Information
- Application Number
- CN202422484761.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-14
- Publication Date
- 2025-09-09
- Estimated Expiration
- 2034-10-14
AI Technical Summary
When preparing cell slides on existing six-well cell culture plates, the cover glass easily adheres to the bottom of the culture plate due to capillary adsorption, making it difficult to remove. It is also easy to break when picked up with tweezers, affecting the experimental process.
A disposable six-well cell culture plate was designed, which includes a six-well plate shell, a slide climbing trough and a slide climbing clamping ear. One end of the slide climbing trough is connected to the slide climbing inclined trough. The slide climbing clamping ear forms an angle of 30°-45° with the slide climbing inclined trough. The slide climbing inclined trough is set at the front part of the slide climbing trough to reduce the adsorption force. The slide climbing clamping ear is thickened to facilitate the use of tweezers to pick up the slide.
It enables convenient placement and removal of cell slides, avoids damage to the slides due to operational errors, and ensures the accuracy of experimental results.
Smart Images
Figure CN223316698U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell biological experiments, in particular to a disposable six-well cell culture plate for cell climbing slices. Background Art
[0002] When conducting cell biology experiments, since the operation objects are invisible to the naked eye, a variety of experimental consumables are often required. Therefore, for the consumables for cell biology experiments, whether they are easy to operate is undoubtedly the core of the design. Six-well plates are the most commonly used type of experimental consumables in cell biology experiments, and they have been widely used in experiments at various cell levels. Among them, preparing cell slides is a necessary preliminary step for conducting some specific experiments, such as cell immunofluorescence staining, acridine orange-ethidium bromide staining and other experiments. Only after the cell slide model is successfully constructed can subsequent experiments be carried out accurately.
[0003] Due to the invisibility of the operation object and the thinness of the coverslip, some problems that affect the progress of the experiment arise. The preparation of cell slides needs to be carried out in a culture plate such as a six-well plate. When the coverslip is placed in the culture well, due to capillary adsorption, the culture medium adsorbs the coverslip and the bottom of the culture plate together, resulting in the subsequent experiment. The coverslip with fixed cells is difficult to remove from the culture plate; and the tweezers commonly used in biological experiments are very easy to crush the coverslip when it is clamped. Therefore, there is an urgent need to provide a disposable six-well cell culture plate for cell slides, which does not require unnecessary operations and can achieve non-destructive and convenient placement of cell slides using common biological tweezers. Utility Model Content
[0004] In response to the above technical problems, a disposable six-well cell culture plate for cell culture is provided. The technical means adopted by the utility model are as follows:
[0005] A disposable six-well cell culture plate for cell climbing slices comprises a six-well plate shell, a cell climbing slice and a climbing slice groove, one end of the climbing slice groove is connected to a climbing slice inclined groove with a preset inclination angle, one end of the cell climbing slice is connected to a climbing slice clamping ear, the cell climbing slice is arranged in the climbing slice groove, and the climbing slice clamping ear is arranged on the side of the climbing slice groove not connected to the climbing slice inclined groove.
[0006] Furthermore, the cell slide has a diameter of 5-20 mm and a thickness of 0.1-0.5 mm.
[0007] Furthermore, the angle between the cell slide and the slide clamping ear is 30°-45°.
[0008] Furthermore, the width of the climbing sheet clamping ear is 3-5 mm, and the thickness is 2-3 mm.
[0009] Furthermore, the depth of the sheet climbing groove is 0.1-2 mm and the diameter is 20-25 mm.
[0010] Furthermore, the angle between the climbing plate inclined groove and the bottom of the six-hole plate is 30°-45°.
[0011] Furthermore, the depth of the climbing plate inclined groove is 0.1-2 mm.
[0012] Furthermore, six independently opened culture spaces are provided inside the six-well plate shell, and the slice climbing groove is provided at the center position of the culture space.
[0013] The utility model has the following advantages: the utility model has a simple structure and is easy to realize commercial production, and the user can directly inoculate the cells onto the slide after adding the culture medium without any other processing. After the experiment is over, the user can use common biological tweezers to clamp the slide clamping ear. Since it has been thickened, damage to the slide caused by operational errors is avoided. By setting the slide clamping ear and the slide to have an angle of 30°-45°, and providing a slide inclined groove in the front section of the slide groove, on the one hand, it is convenient for the user to use tweezers to clamp, and on the other hand, the adsorption force generated by capillary adsorption is eliminated by the inclined groove, so that it is convenient for the user to take and put the slide without affecting the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.
[0015] Figure 1 This is a schematic diagram of the overall top view of the structure of the utility model;
[0016] Figure 2 This is a schematic diagram of the overall front view structure of the present utility model;
[0017] Figure 3 This is a schematic diagram of the overall left-side structure of the present invention;
[0018] In the figure: 1. Six-well plate shell; 2. Cell slide; 3. Slide clamping ears; 4. Slide tilting groove; 5. Slide groove. DETAILED DESCRIPTION
[0019] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0020] like Figures 1 to 3 As shown, the embodiment of the utility model discloses a disposable six-well cell culture plate for cell climbing, comprising a six-well plate housing 1, a cell climbing sheet 2 and a climbing sheet groove 5, one end of the climbing sheet groove is connected to a climbing sheet inclined groove 4 with a preset inclination angle, one end of the cell climbing sheet is connected to a climbing sheet clamping ear 3, the cell climbing sheet is arranged in the climbing sheet groove, and the climbing sheet clamping ear is arranged on the side of the climbing sheet groove not connected to the climbing sheet inclined groove. The cell climbing sheet is a sterilized cover glass.
[0021] In this embodiment, the diameter of the cell slide is 5-20 mm and the thickness is 0.1-0.5 mm.
[0022] In this embodiment, the angle between the cell slide and the slide clamping ear is 30°-45°.
[0023] In this embodiment, the width of the sheet climbing clamping ear is 3-5 mm, and the thickness is 2-3 mm.
[0024] In this embodiment, the depth of the sheet climbing groove is 0.1-2 mm and the diameter is 20-25 mm.
[0025] In this embodiment, the angle between the climbing plate inclined groove and the bottom of the six-hole plate is 30°-45°.
[0026] In this embodiment, the depth of the climbing plate inclined groove is 0.1-2 mm.
[0027] In this embodiment, the above-mentioned climbing slice clamping ears have a certain height so as to be easily clamped by tweezers. That is, during the culture process, the height of the upper surface of the cell climbing slice matches the highest point of the climbing slice trough. The height of the climbing slice clamping ears is higher than the highest point of the climbing slice trough by a certain distance. The cross-sectional shape of the climbing slice clamping ears matches the cell climbing slice. Figure 1 As shown, the cell slide clamping ears in this embodiment are in an arc shape with a preset arc length, and the arc length is sufficient to be clamped by tweezers, and can be adaptively adjusted according to different cell culture plates and cell slides.
[0028] The angle and thickness design facilitates the use of common laboratory tweezers for cell slide placement. While ensuring the cell slide is positioned within the groove during the experiment, it also increases the relative movement of the groove when gripping the cell slide, eliminating the risk of damaging the sample or crushing the cell slide with tweezers. The tilted groove reduces the adsorption force between the slide and the six-well culture plate, making it easier to place and retrieve the slide.
[0029] In this embodiment, six independently opened culture spaces are provided inside the six-well plate shell, and the slide climbing groove is provided at the center of the culture space.
[0030] During the specific implementation, the user adds 2-3ml of culture medium into the six-well plate shell 1, and then connects a certain concentration of cell suspension to the center of the cell climbing sheet 2. During this period, avoid violent shaking of the six-well plate shell 1. The well-cultured cells will be well adsorbed on the cell climbing sheet. When the user needs to remove the cell climbing sheet, he can use biological tweezers to clamp the climbing sheet clamping ear 3 on one side of the cell climbing sheet 2 and tilt it less than 45° towards the climbing sheet tilting groove 4. After that, the cell climbing sheet 2 can be removed. Experiments that require the preparation of cell climbing sheets as a necessary step can adopt the above operation for use.
[0031] The technical solutions described above have been able to solve the problems existing in the prior art very well. As other scalable implementation methods, when cells are fixed on a coverslip, using tweezers to pick them up can easily cause the cells in the gripping area to fall off or be killed, thereby affecting the experimental results. In addition, the coverslip is not fixed in the six-well plate and may be displaced as the experimenter operates, affecting cell fixation. To this end, a hydrophilic factor can be introduced on the surface of the cell slide to implement TC treatment. Since the hydrophilic factor is introduced into the cell slide, the cultured cells can be relatively stably adsorbed on the slide, avoiding the occurrence of cell shedding due to operation.
[0032] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.
Claims
1. A disposable six-well cell culture plate for cell culture, characterized in that: It includes a six-well plate shell, a cell climbing slide and a climbing slide groove, one end of the climbing slide groove is connected to a climbing slide inclined groove with a preset inclination angle, one end of the cell climbing slide is connected to a climbing slide clamping ear, the cell climbing slide is arranged in the climbing slide groove, and the climbing slide clamping ear is arranged on the side of the climbing slide groove that is not connected to the climbing slide inclined groove.
2. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The cell slide has a diameter of 5-20 mm and a thickness of 0.1-0.5 mm.
3. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The angle between the cell slide and the slide clamping ear is 30°-45°.
4. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The width of the sheet climbing clamping ear is 3-5 mm, and the thickness is 2-3 mm.
5. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The depth of the sheet climbing groove is 0.1-2 mm and the diameter is 20-25 mm.
6. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The included angle between the climbing plate inclined groove and the bottom of the six-hole plate is 30°-45°.
7. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: The depth of the climbing plate inclined groove is 0.1-2 mm.
8. The disposable six-well cell culture plate for cell culture according to claim 1, characterized in that: Six independently opened culture spaces are arranged inside the six-well plate shell, and the slice climbing groove is arranged at the center of the culture space.