Portable biological macromolecular solution concentration device

Abstract

The utility model provides a portable biomacromolecule solution concentration device, which belongs to the field of biomacromolecules, and comprises a hypertonic solution box, a negative pressure pipe is arranged on one side of the hypertonic solution box in a penetrating manner, a semi-permeable filter membrane is arranged in the negative pressure pipe, a negative pressure component is arranged on one side of the hypertonic solution box, and the negative pressure component is arranged on the other side of the hypertonic solution box. A mounting cylinder is arranged above the hypertonic solution box, a containing cylinder is arranged on the mounting cylinder, a sealing cover is arranged above the containing cylinder, a limiting ring is fixedly arranged in the mounting cylinder, a dialysis membrane is arranged in the mounting cylinder, and an annular plate is fixedly arranged at the bottom end of the inner side of the containing cylinder. Separation equipment such as a large-scale centrifugal machine and the like are not needed, heating is not needed, biomacromolecules cannot lose biological activity due to temperature rise, so that the effects of portability and rapid and effective concentration are achieved, the practicability is improved, the negative pressure assembly is further arranged, the flowing rate of water molecules is increased, and the concentration efficiency is improved. The concentration efficiency of the biological macromolecules is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological macromolecules, in particular to a portable biological macromolecule solution concentrating device. Background Art

[0002] Purifying biomacromolecules is a crucial step in biomedical research and the pharmaceutical industry. Biomacromolecules, such as proteins, nucleic acids, or polysaccharides, are often present in trace amounts in complex biological samples. To study their structure and function or serve as a basis for drug development, they need to be separated from other molecules and purified. Concentration is essential in this purification process. First, concentration increases the concentration of the target molecule and reduces sample volume, thereby simplifying subsequent separation steps such as chromatography or electrophoresis. Second, concentration helps reduce impurities in the sample and improve purity, which is crucial for accurate biochemical analysis and structural studies. Furthermore, concentration prevents degradation or aggregation of biomacromolecules at low concentrations, maintaining their stability and activity. Existing techniques for concentrating biomacromolecule solutions include centrifugation and evaporation. The selection of the appropriate concentration method depends on the characteristics of the target molecule and the desired purity level. Proper execution of the concentration step is crucial to ensuring the integrity and functionality of the biomacromolecule and is the foundation for subsequent research and applications.

[0003] Among the existing biomacromolecule concentration technologies, centrifugation and evaporation require large equipment or heating equipment to operate, which is very inconvenient to use. In addition, increasing the temperature will lead to a decrease in the activity of biomacromolecules, reducing practicality. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model provides a portable biomacromolecule solution concentrating device.

[0005] The embodiment of the present utility model provides a portable biomacromolecule solution concentration device, comprising:

[0006] A hypertonic solution box, wherein a negative pressure tube is provided through one side of the hypertonic solution box, a semipermeable filter membrane is provided in the negative pressure tube, a negative pressure component is provided on one side of the hypertonic solution box, a mounting tube is provided above the hypertonic solution box, a holding tube is provided on the mounting tube, a sealing cover is provided above the holding tube, a limiting ring is fixedly provided in the mounting tube, a dialysis membrane is provided in the mounting tube, an annular plate is fixedly provided at the inner bottom end of the holding tube, and the annular plate is provided in cooperation with the limiting ring.

[0007] Furthermore, the negative pressure assembly includes a piston cylinder, a piston, a first one-way valve, a delivery pipe and a second one-way valve. The piston is slidably arranged in the piston cylinder, a connecting rod is fixedly arranged on one side of the piston, the connecting rod slides through the outer end of the piston cylinder, the first one-way valve is fixedly arranged through the inner end of the piston cylinder, the delivery pipe is fixedly connected to the outer end of the first one-way valve, and the second one-way valve is fixedly arranged through one side of the piston cylinder.

[0008] Furthermore, the outer bottom end of the mounting tube and both outer ends of the containing tube are provided with external threads, and the inner top end of the hypertonic solution box and the mounting tube and the inner bottom end of the sealing cover are provided with internal threads.

[0009] Furthermore, the dialysis membrane is arranged between the limiting ring and the annular plate, and a sealing gasket is fixedly arranged on the bottom of the annular plate, and the sealing gasket is arranged to abut against the dialysis membrane.

[0010] Furthermore, a rubber ring is fixedly provided on the outer side of the delivery pipe, and the rubber ring is provided in cooperation with the negative pressure pipe.

[0011] Furthermore, an air inlet hole is provided through the outer side of the piston cylinder, and a handle is fixedly provided on the outer end of the connecting rod.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] 1. A hypertonic solution box and dialysis membrane are provided. Due to the concentration difference between the biomacromolecule sample to be concentrated in the holding cylinder and the hypertonic solution in the hypertonic solution box on the other side of the dialysis membrane, water molecules in the holding cylinder naturally flow into the hypertonic solution box. At the same time, under the action of gravity, the flow speed is also increased. There is no need to use large separation equipment such as large centrifuges, and there is no need for heating. The biomacromolecules will not lose their biological activity due to the increase in temperature, thus achieving the effect of portability and rapid and effective concentration.

[0014] 2. A negative pressure component is provided. By pulling the handle back and forth, the piston is driven to move back and forth through the connecting rod. The piston can extract water from the hypertonic solution box through the first and second one-way valves, thereby generating negative pressure in the hypertonic solution box, increasing the flow rate of water molecules in the cylinder to the hypertonic solution box, and thus improving the concentration efficiency of biological macromolecules.

[0015] In summary, the present invention does not require the use of separation equipment such as large centrifuges, nor does it require heating. Biomacromolecules will not lose their biological activity due to increased temperature, thereby achieving the effect of portability and rapid and effective concentration, increasing practicality. A negative pressure component is also provided to increase the flow rate of water molecules and improve the concentration efficiency of biomacromolecules. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a three-dimensional expanded view of a portable biomacromolecule solution concentration device described in an embodiment of the present utility model.

[0017] Figure 2 This is a partial cross-sectional view of a portable biomacromolecule solution concentration device described in an embodiment of the present utility model.

[0018] Figure 3 This is a cross-sectional view of a negative pressure component in a portable biomacromolecule solution concentration device described in an embodiment of the present utility model.

[0019] In the above drawings: 1 hypertonic solution box, 2 mounting cylinder, 3 holding cylinder, 4 sealing cover, 5 dialysis membrane, 6 limiting ring, 7 ring plate, 8 sealing gasket, 9 external thread, 10 internal thread, 11 negative pressure tube, 12 piston cylinder, 13 piston, 14 connecting rod, 15 handle, 16 first one-way valve, 17 delivery tube, 18 rubber ring, 19 second one-way valve. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further described below with reference to the accompanying drawings and embodiments.

[0021] like Figure 1-Figure 3 As shown, the embodiment of the present invention provides a portable biomacromolecule solution concentration device, comprising:

[0022] A hypertonic solution box 1 is used to be filled with a hypertonic solution. The hypertonic solution includes but is not limited to a high-concentration water-soluble polymer solution, including a high-concentration aqueous solution (10-50% concentration) of PEG polyethylene glycol, PVA polyvinyl alcohol, or polyvinyl pyrrolidone. A negative pressure tube 11 is provided on one side of the hypertonic solution box 1. A semipermeable filter membrane is provided in the negative pressure tube 11. The semipermeable filter membrane can intercept and filter the polyethylene glycol molecules in the hypertonic solution to prevent the polyethylene glycol molecules from being sucked out, thereby sucking out only water.

[0023] A negative pressure assembly is provided on one side of the hypertonic solution box 1. The negative pressure assembly includes a piston cylinder 12, a piston 13, a first one-way valve 16, a delivery tube 17, and a second one-way valve 19. An air inlet is provided on the outside of the piston cylinder 12 for air inlet and outlet of the piston cylinder 12. The piston 13 is slidably provided in the piston cylinder 12. A connecting rod 14 is fixedly provided on one side of the piston 13. The connecting rod 14 is slidably provided through the outer end of the piston cylinder 12. A handle 15 is fixedly provided on the outer end of the connecting rod 14. The handle 15 facilitates the staff to pull the piston 13.

[0024] A first one-way valve 16 is fixedly provided through the inner end of the piston cylinder 12, and a delivery pipe 17 is fixedly connected to the outer end of the first one-way valve 16. The first one-way valve 16 can only allow liquid and gas to enter the piston cylinder 12 from the delivery pipe 17. A rubber ring 18 is fixedly provided on the outer side of the delivery pipe 17. The rubber ring 18 is provided in cooperation with the negative pressure pipe 11. The delivery pipe 17 can be inserted into the negative pressure pipe 11. The rubber ring 18 can be tightly pressed against the inner wall of the negative pressure pipe 11, thereby playing a sealing role. A second one-way valve 19 is fixedly provided through one side of the piston cylinder 12. The second one-way valve 19 can only allow liquid and gas to be discharged from the piston cylinder 12;

[0025] A mounting tube 2 is provided above the hypertonic solution box 1, a holding tube 3 is provided on the mounting tube 2, a sealing cover 4 is provided above the holding tube 3, an outer bottom end of the mounting tube 2 and both outer ends of the holding tube 3 are provided with external threads 9, and the inner top ends of the hypertonic solution box 1 and the mounting tube 2 and the inner bottom end of the sealing cover 4 are provided with internal threads 10, so that the bottom of the sealing cover 4 can be threadedly connected to the top of the holding tube 3, the bottom of the holding tube 3 can be threadedly connected to the top of the mounting tube 2, and the bottom of the mounting tube 2 can be threadedly connected to the top of the hypertonic solution box 1;

[0026] A limiting ring 6 is fixedly provided in the mounting tube 2, and the limiting ring 6 serves to limit the dialysis membrane 5. The mounting tube 2 is provided with a dialysis membrane 5, and the dialysis membrane 5 can be a dialysis membrane 5 for separating different molecular weights, including but not limited to dialysis membranes 5 with molecular weights of 2kDa, 3.5kDa, 7kDa, 10kDa and 20kDa. An annular plate 7 is fixedly provided at the inner bottom end of the containing tube 3, and the annular plate 7 is cooperated with the limiting ring 6 and is set against the inner wall of the mounting tube 2. The dialysis membrane 5 is arranged between the limiting ring 6 and the annular plate 7, so that the annular plate 7 can press and fix the dialysis membrane 5. Unscrew the containing tube 3, separate the annular plate 7 from the mounting tube 2, and take out the dialysis membrane 5 for replacement. A sealing gasket 8 is fixedly provided at the bottom of the annular plate 7, and the sealing gasket 8 is set against the dialysis membrane 5, and the sealing gasket 8 serves as a seal.

[0027] The detailed working process of this utility model is as follows:

[0028] 1. When in use, the staff first inserts the delivery tube 17 into the negative pressure tube 11, then fills the hypertonic solution box 1 with hypertonic solution, then covers the installation tube 2 and the holding tube 3, and then injects the biomacromolecule sample to be concentrated into the holding tube 3, and finally covers the sealing cover 4 to start the concentration operation;

[0029] 2. Due to the concentration difference between the biomacromolecule sample to be concentrated in the holding cylinder 3 and the hypertonic solution in the hypertonic solution box 1 on the other side of the dialysis membrane 5, the water molecules in the holding cylinder 3 naturally flow into the hypertonic solution box 1. At the same time, under the action of gravity, the flow rate also increases.

[0030] 3. At the same time, the staff can pull the handle 15 back and forth, driving the piston 13 to move back and forth through the connecting rod 14. The piston 13 can suck the water in the hypertonic solution box 1 into the piston cylinder 12 through the delivery tube 17 and the first one-way valve 16, and then discharge it through the second one-way valve 19, thereby generating a negative pressure in the hypertonic solution box 1, increasing the flow rate of water molecules in the holding tube 3 to the hypertonic solution box 1, and thus improving the concentration efficiency of biological macromolecules.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model and are not limiting. Although the utility model is described in detail with reference to the preferred embodiments, ordinary technicians in this field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A portable biomacromolecule solution concentration device, characterized in that: include: A hypertonic solution box (1), wherein a negative pressure tube (11) is provided on one side of the hypertonic solution box (1), a semipermeable filter membrane is provided in the negative pressure tube (11), a negative pressure assembly is provided on one side of the hypertonic solution box (1), a mounting tube (2) is provided above the hypertonic solution box (1), a holding tube (3) is provided on the mounting tube (2), a sealing cover (4) is provided above the holding tube (3), a limiting ring (6) is fixedly provided in the mounting tube (2), a dialysis membrane (5) is provided in the mounting tube (2), an annular plate (7) is fixedly provided at the inner bottom end of the holding tube (3), and the annular plate (7) is provided in cooperation with the limiting ring (6).

2. A portable biomacromolecule solution concentration device according to claim 1, characterized in that: in: The negative pressure assembly includes a piston cylinder (12), a piston (13), a first one-way valve (16), a delivery pipe (17) and a second one-way valve (19); the piston (13) is slidably arranged in the piston cylinder (12); a connecting rod (14) is fixedly arranged on one side of the piston (13); the connecting rod (14) is slidably arranged through the outer end of the piston cylinder (12); the first one-way valve (16) is fixedly arranged through the inner end of the piston cylinder (12); the delivery pipe (17) is fixedly connected to the outer end of the first one-way valve (16); and the second one-way valve (19) is fixedly arranged through one side of the piston cylinder (12).

3. The portable biomacromolecule solution concentrator according to claim 1, characterized in that: in: The outer bottom end of the mounting tube (2) and the outer ends of the containing tube (3) are both provided with external threads (9), and the inner top end of the hypertonic solution box (1) and the mounting tube (2) and the inner bottom end of the sealing cover (4) are both provided with internal threads (10).

4. The portable biomacromolecule solution concentrator according to claim 1, characterized in that: in: The dialysis membrane (5) is arranged between the limiting ring (6) and the annular plate (7), and a sealing gasket (8) is fixedly arranged at the bottom of the annular plate (7), and the sealing gasket (8) is arranged to abut against the dialysis membrane (5).

5. The portable biomacromolecule solution concentrator according to claim 2, characterized in that: in: A rubber ring (18) is fixedly provided on the outer side of the delivery pipe (17), and the rubber ring (18) is arranged in cooperation with the negative pressure pipe (11).

6. The portable biomacromolecule solution concentrator according to claim 2, characterized in that: in: An air inlet hole is provided through the outer side of the piston cylinder (12), and a handle (15) is fixedly provided on the outer end of the connecting rod (14).

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