Pipeline system for cell cryopreservation bag resuscitation

Abstract

The utility model discloses a pipeline system for cell cryopreservation bag resuscitation, belongs to the technical field of cell resuscitation, and aims to solve the problems that cryopreservation bags are long in resuscitation time and many in opening operation. The pipeline system comprises a puncture part, an emptying part, a screwed bottle connecting part, a screwed bottle part and a pressure vacuum pump part, the puncture part comprises a Luer joint and a pipeline clamp, the emptying part comprises a pipeline clamp, an air filter and a medical silicone tube, the screwed bottle connecting part comprises a three-way joint and a medical silicone tube, and the pressure vacuum pump part is connected with the screwed bottle connecting part. The screwed bottle part comprises a glass screwed bottle, a double-hole screwed bottle cap and an air filter, and the pressure vacuum pump part comprises a medical silicone tube and a pressure vacuum pump. The device is suitable for cryopreservation bags of various specifications, the reduction of cell activity during resuscitation is avoided, open operation is reduced, and the risk of microbial contamination is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell resuscitation, and relates to the efficiency of resuscitation and improving the quality of resuscitated cells. Background Art

[0002] The use of cell cryopreservation bags is a common requirement in cell culture. Disposable infusion tubing or disposable syringes are often used during the cell resuscitation step. Disposable infusion tubing has a slow flow rate, which can easily damage cells and hinder cell viability. While disposable syringes can complete a single draw from small cryopreservation bags, larger bags require multiple draws. Inserting and removing syringes involves open-ended procedures, which can easily lead to contamination. Furthermore, slow flow rates can also damage cells, hindering cell survival. Utility Model Content

[0003] In view of the shortcomings of the current cryobag resuscitation method, the utility model aims to solve the problems of long cryobag resuscitation time and multiple opening operations, and provides a pipeline system for cryobag resuscitation that is simple to operate, has good sealing performance, and is safe and fast. The specific technical solution is as follows:

[0004] A piping system for thawing a cell cryopreservation bag is characterized by comprising a puncture portion connectable to a cryopreservation bag body 1, an emptying portion, a thread-cap bottle connection portion, a thread-cap bottle portion, and a pressure vacuum pump portion. The cryopreservation bag can be any commercially available cryopreservation bag of various specifications.

[0005] Furthermore, the puncture part includes a puncture needle 2 and a Luer connector 3 connected thereto, the Luer connector 3 is connected to a medical silicone tube A 4, on which is a pipe clamp A 5; the emptying part includes a medical silicone tube B 7 and a pipe clamp B 8 thereon, and an air filter A 9; the thread-mouth bottle connection part includes a three-way connector 6 and a medical silicone tube C 10, wherein the three-way connector 6 connects the medical silicone tube A 4, the medical silicone tube B 7 and the medical silicone tube C 10 together, and the medical silicone tube C 10 is connected to the double-hole thread-mouth bottle cap 11 of the thread-mouth bottle part; the thread-mouth bottle part includes a thread-mouth bottle 12, a double-hole thread-mouth bottle cap 11 and an air filter B 13 connected thereto; the pressure vacuum pump part includes a pressure vacuum pump 16 and a medical silicone tube D 14 connected to the negative pressure end 15 of the pressure vacuum pump.

[0006] Furthermore: the puncture needle of the puncture part is sterilized for single use, and the Luer connector, pipeline clamp and medical silicone tube are all made of materials that can be repeatedly sterilized with wet heat.

[0007] Furthermore, the medical silicone tube, pipe clamp and air filter of the evacuation part are all made of materials that can be repeatedly sterilized with wet heat.

[0008] Furthermore: the three-way connector of the thread-mouth bottle connection part and the medical silicone tube are both made of materials that can be repeatedly sterilized with wet heat.

[0009] Furthermore, the glass thread-mouth bottle, the double-hole thread-mouth bottle cap and the air filter of the thread-mouth bottle are all made of materials that can be repeatedly sterilized with wet heat, and the thread-mouth bottle can be filled with cell culture medium or other solutions required for cell recovery in advance.

[0010] Furthermore: the medical silicone tube of the pressure vacuum pump is made of a material that can be repeatedly sterilized by wet heat, and the pressure vacuum pump is a commercially available device.

[0011] When using the piping system of the present invention, puncture needle 2 is inserted into cryopreservation bag body 1, pipe clamp A5 is opened, and three-way connector 6 is adjusted to connect medical silicone tube A4 and medical silicone tube C10, while blocking medical silicone tube 7. Liquid from the cryopreservation bag will now flow into the thread-top bottle. At this point, the pressure vacuum pump 16 is activated to rapidly facilitate the flow of liquid from the cryopreservation bag into the thread-top bottle. Once all the liquid from the cryopreservation bag has flowed into medical silicone tube C10, pipe clamp A5 is closed, pipe clamp B8 is opened, and three-way connector 6 is adjusted to connect medical silicone tube B7 and medical silicone tube C10. Negative pressure suction is then continued to completely drain the liquid from medical silicone tube C10 into the thread-top bottle.

[0012] The cell suspension in the silk-top flask can be inoculated into a culture container through a piping system well known to those skilled in the art.

[0013] Compared with the existing cryopreservation bag resuscitation method, the beneficial effects of the utility model are:

[0014] 1. The piping system is a closed operation, reducing the risk of contamination;

[0015] 2. Connect a pressure vacuum pump to shorten the recovery process, which not only saves time but also is more conducive to cell survival;

[0016] 3. An emptying part is set up to ensure that all cell suspension is used for subsequent inoculation without wasting cells;

[0017] 4. The pipe connection is simple, can be sterilized repeatedly, and is economical. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of the utility model;

[0019] In the figure, 1. Cryopreservation bag body; 2. Puncture needle; 3. Luer connector; 4. Medical silicone tube A; 5. Pipe clamp A; 6. Tee connector; 7. Medical silicone tube B; 8. Pipe clamp B; 9. Air filter A; 10. Medical silicone tube C; 11. Double-hole threaded bottle cap; 12. Threaded bottle; 13. Air filter B; 14. Medical silicone tube D; 15. Negative pressure end of pressure vacuum pump; 16. Pressure vacuum pump. DETAILED DESCRIPTION

[0020] The following will be combined with the embodiments of the present invention and the accompanying drawings to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0021] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "connected" and "connection" should be understood in a broad sense. For example, it can mean a fixed connection, a detachable connection, or an integral connection; it can mean a mechanical connection; it can mean a direct connection, an indirect connection through an intermediate medium, or internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.

[0022] Example

[0023] A piping system for reviving a cell cryopreservation bag comprises a puncture portion, an emptying portion, a thread-cap bottle connection portion, a thread-cap bottle portion, and a pressure vacuum pump portion, which can be connected to a cryopreservation bag body 1; wherein the puncture portion comprises a puncture needle 2 and a Luer connector 3 connected thereto, the Luer connector 3 being connected to a medical silicone tube A 4 with a pipe clamp A 5 disposed thereon; the emptying portion comprises a medical silicone tube B 7, a pipe clamp B 8 disposed thereon, and an air filter A 9; the thread-cap bottle connection portion comprises a three-way connector 6 and a medical silicone tube C 10, wherein the three-way connector 6 connects the medical silicone tubes A 4, B 7, and C 10 together, and the medical silicone tube C 10 is connected to a double-hole thread-cap bottle cap 11 of the thread-cap bottle portion; the thread-cap bottle portion comprises a thread-cap bottle 12, a double-hole thread-cap bottle cap 11, and an air filter B 7 connected thereto. 13; The pressure vacuum pump portion includes a pressure vacuum pump 16 and a medical silicone tube D 14 connected to the negative pressure end 15 of the pressure vacuum pump.

[0024] Among them, all components of the pipeline system except the pressure vacuum pump are sterilized or aseptic.

[0025] Instructions for use: During resuscitation, remove the outer packaging bag after the freezing bag body is thawed, clamp the pipeline system pipeline clamp B 8 and pipeline clamp A 5 of the utility model, connect the puncture needle 2 to the liquid outlet of the freezing bag body 1, adjust the three-way joint 6 to make the medical silicone tube A 4 and the medical silicone tube C 10 communicate, and not communicate with the medical silicone tube B 7, connect the medical silicone tube D 14 to the air filter B 13 and the negative pressure end of the pressure vacuum pump, open the pipeline clamp A 5, turn on the power switch of the pressure vacuum pump 16 to draw negative pressure, when there is no residual liquid in the bag body 1, there is still residual liquid in the medical silicone tube C 10, at this time clamp the pipeline clamp A 5, open the pipeline clamp B 8, and adjust the three-way joint 6 to make the medical silicone tube B 7 and the medical silicone tube C 10 communicate, and then continue to suck the medical silicone tube C 14 with negative pressure. The liquid in 10 is completely drained into the thread-mouth bottle to ensure that there is no residual liquid in the pipeline and reduce cell loss.

[0026] It is obvious that those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A piping system for thawing cell cryopreservation bags, characterized in that The invention comprises a puncture part, an emptying part, a thread bottle connection part, a thread bottle part and a pressure vacuum pump part which can be connected to a freezing bag body (1); wherein the puncture part comprises a puncture needle (2) and a Luer connector (3) connected thereto, the Luer connector (3) is connected to a medical silicone tube A (4) on which a pipe clamp A (5) is arranged; the emptying part comprises a medical silicone tube B (7) and a pipe clamp B (8) thereon and an air filter A (9); the thread bottle connection part comprises a three-way connector (6) and a medical silicone tube C (10), wherein a three-way connector (6) connects the medical silicone tube A (4), the medical silicone tube B (7) and the medical silicone tube C (10) together, and the medical silicone tube C (10) is connected to the double-hole threaded bottle cap (11) of the threaded bottle portion; the threaded bottle portion includes a threaded bottle (12), a double-hole threaded bottle cap (11) and an air filter B (13) connected thereto; the pressure vacuum pump portion includes a pressure vacuum pump (16) and a medical silicone tube D (14) connected to the negative pressure end (15) of the pressure vacuum pump.

2. The piping system for thawing a cell cryopreservation bag according to claim 1, characterized in that: The puncture needle (2) of the puncture part can be connected to the bag body of a freezing bag of various specifications by puncture.

3. The piping system for thawing a cell cryopreservation bag according to claim 1, characterized in that: The outflow of the liquid in the freezing bag can be controlled by controlling the opening and closing of the pipeline clamp A (5) on the medical silicone tube A (4) of the puncture part.

4. The piping system for thawing a cell cryopreservation bag according to claim 1, characterized in that: By controlling the three-way connector (6) and the pipe clamp B (8), the air filter A (9) and the medical silicone tube B (7) and the medical silicone tube C (10) can be connected to each other, so that the solution in the medical silicone tube C (10) can be emptied into the thread-mouth bottle (12).

5. The piping system for thawing a cell cryopreservation bag according to claim 1, characterized in that: The air filter B (13) is connected to the negative pressure end (15) of the pressure vacuum pump (16) through the medical silicone tube D (14) to pump negative pressure so that the liquid in the cryopreservation bag flows into the wire-mouth bottle (12) faster.

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