Injector device in animal brain

Abstract

The utility model discloses a syringe device in an animal brain. The syringe device comprises two microsyringes, an injection push cap, a first fixator, a second fixator and a needle pitch fixator, each of the two microsyringes comprises a needle cylinder, a piston and a needle body, one end, with a piston head, of the piston is arranged in the needle cylinder and can slide on the inner wall of the needle cylinder, the other end of the piston is sleeved with the injection push cap, and the two microsyringes are fixed through the injection push cap and the first fixator. The two needle bodies are arranged in the syringe device, the needle distance fixer fixes the distance between the two needle bodies at a required distance, and the two microsyringes are ensured to be parallel and stable, so that the defect that the existing microsyringes can only inject medicines on one side is greatly improved, and the injection push cap of the syringe device is pushed manually or by other automatic propulsion devices, so that the injection efficiency is greatly improved. Therefore, simultaneous injection in the brains of the two sides of the animal can be realized, experimental operation time is obviously reduced, and animal death rate is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of intracerebral injection of animals in biomedical laboratories, and in particular to an intracerebral injector device for animals. Background Art

[0002] Existing microsyringes are all individually packaged. Taking the injection of drugs into the dentate gyrus of the bilateral hippocampus of mice as an example, when using existing microsyringes, first use a brain stereotaxic instrument to locate the mouse skull 2.0 mm posterior to the anterior fontanelle and 1.4 mm lateral to both sides and drill holes (the distance between the two holes is 2.8 mm). By moving the piston to draw out an appropriate amount of drug, the microsyringe barrel is fixed to the brain stereotaxic instrument, and the microsyringe needle body is inserted 2.5 mm into the drilled hole on one side. The piston of the microsyringe is pushed manually or by other automatic propulsion devices to perform drug injection.

[0003] However, when using a brain stereotaxic apparatus for bilateral injection into the animal's brain, existing microinjectors require positioning twice in the left and right hemispheres of the brain, resulting in many experimental steps, a long time, and a high risk of animal death.

[0004] In order to solve the above-mentioned technical deficiencies of the prior art, it is necessary to design an animal brain intracerebral injector device. Utility Model Content

[0005] The purpose of the utility model is to solve the shortcomings of the prior art and to propose an animal brain injector device.

[0006] The syringe is inserted into the syringe and the syringe is inserted into the syringe. The syringe is inserted into the syringe and the syringe is inserted into the syringe. The syringe is inserted into the syringe and the syringe is inserted into the syringe.

[0007] Furthermore, a metal handle is provided at the tail end of the syringe, an anti-rotation block is fixedly sleeved on the metal handle, and the anti-rotation block is fixedly connected to the first fixture on a side close to the first fixture.

[0008] Furthermore, the diameter of the needle distance fixing hole is adapted to the diameter of the needle body. Beneficial effects

[0009] The utility model fixes two micro-syringes by means of an injection push cap and a first fixator, further fixes them by means of a fixing section of a second fixator, and finally fixes the distance between the two needle bodies at a desired distance by means of a needle distance fixator, thereby ensuring that the two micro-syringes are parallel and stable, thereby greatly improving the disadvantage that the existing micro-syringes can only inject drugs on one side. By pushing the injection push cap of the syringe device manually or by means of an automatic propulsion device, simultaneous injection into the bilateral brains of animals can be achieved, thereby significantly reducing experimental operation time and lowering animal mortality.

[0010] The utility model ensures the stability of the overall structure of the syringe by performing double fixation through the fixing section and the first fixator. The straight channel opened on the fixing section prevents the needle body from bending while ensuring smooth flow of medicine in the syringe. The curved arc path arranged on the guide section bends the syringe needle body with a certain curvature, which can ensure that the medicine can smoothly reach the needle tip from the syringe during the experiment, avoiding the situation of drug blockage, and has low cost, simple operation and high injection efficiency.

[0011] The thrust of the injection push cap enables the two pistons to maintain the same speed when pushing the drug, that is, to ensure that the two micro-syringes inject the same amount synchronously, thereby greatly improving the disadvantage of the existing syringes that the speed of bilateral drug injection is not uniform when in use, affecting the accuracy of the experiment. The needle distance fixer can fix the needle tips of the two needle bodies at the required distance, reduce the injection operation steps, and improve the accuracy of the positioned injection. At the same time, the needle distance fixer can reinforce the needle body. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a front view of the animal brain injection device of the utility model;

[0013] Figure 2 This is a cross-sectional view of the injection push cap, the first fixer, and the second fixer of the utility model;

[0014] Figure 3 This is a cross-sectional view of the second fixator of this utility model;

[0015] Figure 4 This is a comparison chart of experimental data of this utility model;

[0016] In the picture:

[0017] Microinjector 1; syringe 11; piston 12; needle body 13; injection push cap 2; first fixer 3; second fixer 4; fixing section 4a; guide section 4b; needle distance fixer 5. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] This example uses the injection of drugs into the dentate gyrus of the bilateral hippocampus of mice as an example for the following description. Figure 1-Figure 4 The present embodiment proposes an animal brain injection device, comprising two micro-syringes 1, an injection push cap 2, a first fixer 3, a second fixer 4 and a needle distance fixer 5, wherein the injection push cap 2, the first fixer 3, the second fixer 4 and the needle distance fixer 5 are all fixed by AB glue, the capacity of each micro-syringe 1 is 10 μl, and the injection push cap 2, the first fixer 3, the second fixer 4 and the needle distance fixer 5 are all printed by a 3D printer using Polylactic Acid (polylactic acid) bioplastic; the two micro-syringes 1 each comprise a syringe 11, a piston 12 and a needle body 13, one end of the piston 12 with the piston head is arranged in the syringe 11 and can slide on its inner wall, the injection push cap 2 is sleeved on the other end of the piston 12, and the thrust of the injection push cap 2 enables the two pistons 12 to maintain the same speed when pushing the drug, that is, to ensure that the two micro-syringes 1 inject the same amount synchronously, thereby greatly improving the problem of bilateral injection of drugs by existing syringes during use. The disadvantage of inconsistency affects the accuracy of the experiment. The first fixer 3 is fixedly sleeved on the tail end of the syringe 11, and the needle body 13 is arranged at the front end of the syringe 11. The first fixer 3 is used to fix the syringe 11. The tail end of the syringe 11 is also provided with a metal handle. The metal handle is fixedly sleeved with an anti-rotation block. The side of the anti-rotation block close to the first fixer 3 is fixedly connected to the first fixer 3. The anti-rotation block wraps the metal handle on the syringe 11. The same anti-rotation block is fixedly sleeved by the two metal handles, so that the connection between the two syringes 11 is stable and the two syringes 11 are prevented from rotating.

[0020] The second holder 4 includes a fixed section 4a and a guide section 4b. Two straight channels are provided on the fixed section 4a. The fixed section 4a is fixedly sleeved on the front end of the syringe 11 so that the needle body 13 passes through the straight channels. The straight channels provided on the fixed section 4a prevent the needle body 13 from bending while ensuring smooth flow of the medicine in the syringe 11. Two curved arc channels are provided on the guide section 4b. The guide section 4b is fixed on the side of the fixed section 4a away from the syringe 11 so that the needle body 13 passes through the curved arc channels. By setting the curved arc channel, the syringe needle body 13 is bent at a certain curvature, which can ensure that the medicine can smoothly reach the needle tip from the needle tube during the experiment. To avoid drug blockage, the needle distance holder 5 is provided with two needle distance fixing holes. The needle distance holder 5 is fixedly sleeved on the needle body 13 away from the syringe 11, so that the needle body 13 passes through the needle distance fixing hole to reduce the distance between the two needle bodies 13. The diameter of the needle distance fixing hole is adapted to the diameter of the needle body 13. In this embodiment, the fixed distance of the needle distance holder 5 is set to 2.8 mm for injection into the dentate gyrus of the bilateral hippocampus of mice. The needle distance holder 5 can fix the needle tips of the two needle bodies 13 at the required distance, reduce the injection operation steps, and improve the accuracy of the positioned injection. At the same time, the needle distance holder 5 can reinforce the needle body 13.

[0021] The utility model fixes two micro-syringes 1 by the injection push cap 2 and the first fixer 3, and then further fixes them by the fixing section 4a of the second fixer 4, and finally fixes the distance between the two needle bodies 13 at the required distance by the needle distance fixer 5, ensuring that the two micro-syringes 1 are parallel and stable, thereby greatly improving the disadvantage that the existing micro-syringes can only inject drugs on one side. By pushing the injection push cap 2 of the syringe device manually or by other automatic propulsion devices, simultaneous injection into the bilateral brains of animals can be achieved, which significantly reduces the experimental operation time and reduces the animal mortality rate. In addition, the fixing section 4a and the first fixer 3 are fixed twice to ensure the stability of the overall structure of the syringe. The straight channel opened on the fixing section 4a avoids the bending of the needle body 13 while ensuring the smooth flow of the drug in the syringe 11. The curved arc path set on the guide section 4b bends the syringe needle body 13 with a certain curvature, further ensuring that the drug can smoothly reach the needle tip from the syringe during the experiment, avoiding the situation of drug blockage, and has low cost, simple operation and high injection efficiency.

[0022] The method of using the utility model is as follows:

[0023] Taking bilateral drug injection into the dentate gyrus of the mouse hippocampus as an example, first use a brain stereotaxic instrument to locate the mouse skull 2.0 mm posterior to the anterior bregma and 1.4 mm lateral to the sides and drill holes (2.8 mm apart). Fix the injection device that draws an appropriate amount of drug to the brain stereotaxic instrument, and insert the needle 2.5 mm into the drill hole. Push the injection push cap 2 of the syringe device manually or with another automatic propulsion device to start the simultaneous injection into the bilateral hippocampus.

[0024] like Figure 4 As shown, Figure 4 A is a sagittal section of brain tissue from a normal mouse stained with hematoxylin-eosin (HE). Figure 4 B is a hematoxylin-eosin (HE) stained image of a sagittal section of brain tissue in which granule cell apoptosis was induced by injection of amyloid-β protein (Aβ) into the bilateral dentate gyrus of the hippocampus of mice. By using the utility model to perform intracerebral injection in laboratory animals, the shortcomings of the existing bilateral intracerebral injection experiments using microsyringes, such as multiple operating steps, long time, and high risk of animal death, are solved, and the utility model has the advantages of stability and precision.

[0025] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An animal brain injection device, characterized in that: The invention comprises two micro-syringes (1), an injection push cap (2), a first fixer (3), a second fixer (4) and a needle distance fixer (5); the two micro-syringes (1) each comprise a syringe (11), a piston (12) and a needle body (13); one end of the piston (12) with a piston head is arranged in the syringe (11) and can slide on the inner wall thereof; the injection push cap (2) is sleeved on the other end of the piston (12); the first fixer (3) is fixedly sleeved on the rear end of the syringe (11); and the needle body (13) is arranged at the front end of the syringe (11); The second holder (4) comprises a fixing section (4a) and a guiding section (4b), wherein the fixing section (4a) is provided with two straight channels, wherein the fixing section (4a) is fixedly sleeved on the front end of the syringe (11) so that the needle body (13) passes through the straight channels, and the guiding section (4b) is provided with two curved arc channels, wherein the guiding section (4b) is fixed on a side of the fixing section (4a) away from the syringe (11) so that the needle body (13) passes through the curved arc channels; The needle distance fixer (5) is provided with two needle distance fixing holes. The needle distance fixer (5) is fixedly sleeved on the needle body (13) away from the needle cylinder (11), so that the needle body (13) passes through the needle distance fixing holes to reduce the distance between the two needle bodies (13).

2. The syringe device according to claim 1, wherein: The tail end of the syringe (11) is also provided with a metal handle, and an anti-rotation block is fixedly sleeved on the metal handle. The anti-rotation block is fixedly connected to the first fixer (3) on a side close to the first fixer (3).

3. The syringe device according to claim 1, wherein: The diameter of the needle distance fixing hole is adapted to the diameter of the needle body (13).

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