Reaction device
By designing a switching structure in the reaction device, the isolation and connection of LAMP/RT-LAMP amplification reaction and CRISPR/Cas12a cutting reaction are achieved, which solves the problem of aerosol pollution and improves the accuracy of diagnosis of major animal diseases.
Patent Information
- Application Number
- CN202421536045.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-02
- Publication Date
- 2025-09-16
- Estimated Expiration
- 2034-07-02
AI Technical Summary
In existing technologies, LAMP/RT-LAMP amplification reactions and CRISPR/Cas12a cleavage reactions need to be performed separately, which leads to the risk of aerosol contamination and affects the accuracy of disease diagnosis results.
A reaction device was designed, including first and second reaction tubes and a switching structure. The isolation and connection of reagents and products were achieved by switching the position of the reversing tube, ensuring that two reactions were completed in the same device and avoiding aerosol contamination.
It effectively reduces the risk of aerosol contamination caused by the overflow of high-concentration amplification products and improves the accuracy of diagnosis of major animal epidemics such as African swine fever and highly pathogenic avian influenza.
Smart Images
Figure CN223342698U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of detection instruments, in particular to a reaction device. Background Art
[0002] Currently, the combination of CRISPR / Cas12a and LAMP / RT-LAMP technology is a common molecular diagnostic method for diagnosing major animal diseases such as African swine fever and highly pathogenic avian influenza.
[0003] In related technologies, the temperatures required for LAMP / RT-LAMP amplification reaction and CRISPR / Cas12a cutting reaction are different, which are around 65°C and 37°C respectively. During operation, researchers usually divide them into two independent units for reaction. In this process, it is necessary to open the LAMP / RT-LAMP amplification reaction tube to remove the target gene fragment for the next CRISPR / Cas12a cutting reaction. The process of removing the target gene fragment is prone to the risk of aerosol contamination, resulting in inaccurate disease diagnosis results. Summary of the Invention
[0004] The main purpose of the utility model is to provide a reaction device, which aims to reduce the risk of aerosol contamination caused by the overflow of high-concentration amplification products during the detection process and improve the accuracy of the diagnosis results of major animal epidemics such as African swine fever and highly pathogenic avian influenza.
[0005] To achieve the above-mentioned purpose, the reaction device proposed in the present invention comprises:
[0006] A first reaction tube is provided with a first sealed cavity and a first connecting channel communicating with the first sealed cavity;
[0007] A second reaction tube is provided with a second sealed cavity and a second connecting channel communicating with the second sealed cavity;
[0008] The switching structure includes a connecting seat and a reversing tube, wherein the first reaction tube and the second reaction tube are connected to opposite sides of the connecting seat and are arranged opposite to each other, and the reversing tube is connected to the connecting seat and has a first position and a second position relative to the connecting seat;
[0009] In the first position, the reversing tube isolates the first connecting channel from the second connecting channel;
[0010] In the second position, the reversing tube connects the first connecting channel and the second connecting channel.
[0011] In an optional embodiment, the connecting seat is formed with a liquid passage and a mounting hole communicating with the liquid passage;
[0012] The reversing tube is rotatably inserted into the mounting hole and can rotate relative to the connecting seat between the first position and the second position, so that the liquid passage connects or separates the first sealed cavity and the second sealed cavity.
[0013] In an optional embodiment, the cross section of the reversing tube is gradually reduced from the middle thereof toward both sides.
[0014] In an optional embodiment, the switching structure further includes a sealing ring, the reversing tube is inserted into the mounting hole, and the sealing ring abuts against the reversing tube and the connecting seat.
[0015] In an optional embodiment, a side wall of the mounting hole is recessed to form a mounting groove, and a portion of the sealing ring is embedded in the mounting groove.
[0016] In an optional embodiment, the sealing ring is made of self-lubricating silicone.
[0017] In an optional embodiment, the switching structure further includes a rotating handle, and the rotating handle is connected to the upper portion of the reversing tube.
[0018] In an optional embodiment, the first reaction tube includes a first tube body, a first connecting tube, and a first sealing cover, wherein the first sealing cover covers the first tube body to enclose and form the first sealed cavity, and the first connecting tube is connected to the first tube body to form the first connecting channel;
[0019] The second reaction tube includes a second tube body, a second connecting tube, and a second sealing cover. The second sealing cover covers the second tube body to form the second sealed cavity. The second connecting tube is connected to the second tube body to form the second connecting channel.
[0020] The first connecting pipe and the second connecting pipe are respectively plugged into opposite sides of the liquid passage.
[0021] In an optional embodiment, the second tube body is located above the connecting seat, and the second connecting tube is inclined toward one side of the connecting seat.
[0022] The reaction device of the present invention includes a first reaction tube, which is provided with a first sealed cavity and a first connecting channel connected to the first sealed cavity; the second reaction tube is provided with a second sealed cavity and a second connecting channel connected to the second sealed cavity; the switching structure includes a connecting seat and a reversing tube; the first reaction tube and the second reaction tube are connected to opposite sides of the connecting seat and are arranged relative to each other; the reversing tube is connected to the connecting seat and has a first position and a second position relative to the connecting seat.
[0023] In the present invention, when it is in the first position, the reversing tube isolates the first connecting channel and the second connecting channel, so that LAMP / RT-LAMP amplification reagent and template can be added to the first sealed cavity to perform LAMP / RT-LAMP amplification reaction, and CRISPR / Cas12a cutting reaction reagent is added to the second sealed cavity. After the amplification reaction is completed, the reversing tube is manipulated to be in the second position relative to the connecting seat, so that the first connecting channel and the second connecting channel are connected, and the CRISPR / Cas12a cutting reaction reagent in the second sealed cavity flows into the first sealed cavity, and performs a cutting reaction on the LAMP / RT-LAMP amplification product in the first sealed cavity. It can be seen from the above process that during the entire detection process, the reagents and LAMP / RT-LAMP amplification products in the first sealed cavity and the second sealed cavity are always isolated from the outside world. Therefore, the risk of aerosol contamination caused by the overflow of high-concentration amplification products during the detection process can be greatly reduced, and the accuracy of the diagnosis results of major animal epidemics such as African swine fever and highly pathogenic avian influenza can be greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.
[0025] Figure 1 This is a schematic structural diagram of an embodiment of the reaction device of the present invention;
[0026] Figure 2 for Figure 1 An exploded view of the reaction apparatus shown;
[0027] Figure 3 for Figure 1 A top view of the reaction apparatus shown;
[0028] Figure 4 for Figure 3 The reaction device shown is a cross-sectional view along AA.
[0029] Description of Figure Numbers:
[0030] Label name Label name 100 reaction device 22 Second connecting pipe 10 First reaction tube 23 Second sealing cover 10a First sealed cavity 30 Switching structure 10b First connection channel 31 Connector 11 first tube body 31a Liquid channel 12 First connecting pipe 31b Mounting holes 13 First sealing cover 31c Mounting card slot 20 Second reaction tube 32 Reversing tube 20a Second sealed cavity 33 sealing ring 20b Second connection channel 34 Turn the handle 21 Second tube body
[0031] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION
[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0033] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.
[0034] In addition, the descriptions of "first," "second," etc. in this utility model are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this utility model.
[0035] Reference Figures 1 to 4 The present invention provides a reaction device 100 .
[0036] In an embodiment of the present invention, the reaction device 100 is primarily used for diagnosing major animal diseases such as African swine fever and highly pathogenic avian influenza. Specifically, the reaction device 100 includes a first reaction tube 10, a second reaction tube 20, and a switching mechanism 30. The first reaction tube 10 includes a first tube body 11, a first connecting tube 12, and a first sealing cap 13. The first tube body 11 and the first connecting tube 12 are integrally blow-molded from a highly transparent polypropylene material. The second tube body 21 and the second connecting tube 22 are also integrally blow-molded from a highly transparent polypropylene material. The first sealing cap 13 covers the open end of the first tube body 11 and, together with the first tube body 11, forms a first sealed cavity 10a for reaction. The first connecting tube 12 forms a first connecting channel 10b. The second sealing cap 23 covers the second tube body 21 and, together with the second tube body 21, forms a second sealed cavity 20a. The second connecting tube 22 forms a second connecting channel 20b.
[0037] The switching structure 30 includes a connecting seat 31 and a reversing tube 32, wherein the connecting seat 31 and the reversing tube 32 can also be made of highly transparent polypropylene. The connecting seat 31 forms a liquid passage 31a for connecting the first connecting channel 10b and the second connecting channel, and a mounting hole 31b for mounting the reversing tube 32. The reversing tube 32 is rotatably inserted into the mounting hole 31b, and the first connecting tube 12 and the second connecting tube 22 are respectively inserted into opposite sides of the liquid passage 31a. That is, in the present application, the first reaction tube 10, the second reaction tube 20 and the connecting seat 31 are detachably connected, which is convenient for production and manufacturing, and also easy to carry. Of course, the first reaction tube 10, the second reaction tube 20 and the connecting seat 31 can also be set by integral molding, so that their sealing performance is better. The reversing tube 32 is inserted into the mounting hole 31b and defines a through-hole (not shown) extending through two opposing surfaces thereof. In the first position, the through-hole communicates with the liquid passage 31a. In the second position, the central axis of the through-hole forms a 90° angle with the central axis of the liquid passage 31a. That is, in this position, the sidewall of the reversing tube 32 blocks the liquid passage 31a. Thus, under the action of an external force, the reversing tube 32 rotates relative to the connecting seat 31 between the first and second positions, thereby enabling the liquid passage 31a to connect or separate the first sealed chamber 10a and the second sealed chamber 20a.
[0038] In the present invention, when located in the first position, the reversing tube 32 isolates the first connecting channel 10b from the second connecting channel 20b, so that LAMP / RT-LAMP amplification reagents and templates can be added to the first sealed cavity 10a to perform LAMP / RT-LAMP amplification reaction, and CRISPR / Cas12a cutting reaction reagents can be added to the second sealed cavity 20a. After the amplification reaction is completed, the reversing tube 32 is manipulated to be in the second position relative to the connecting seat 31, so that the first connecting channel 10b and the second connecting channel 20b are connected, and the CRISPR in the second sealed cavity 20a is connected. The PR / Cas12a cutting reaction reagent flows into the first sealed cavity 10a and performs a cutting reaction on the LAMP / RT-LAMP amplification product in the first sealed cavity 10a. From the above process, it can be seen that during the entire detection process, the reagents and LAMP / RT-LAMP amplification products in the first sealed cavity 10a and the second sealed cavity 20a are always isolated from the outside world. Therefore, the risk of aerosol contamination caused by the overflow of high-concentration amplification products during the detection process can be greatly reduced, and the accuracy of the diagnosis results of major animal epidemics such as African swine fever and highly pathogenic avian influenza is greatly improved.
[0039] Please see again Figure 1Furthermore, the cross-section of the reversing tube 32 gradually decreases from its center toward both sides. That is, the longitudinal cross-section of the reversing tube 32 is elliptical, and correspondingly, the longitudinal cross-section of the mounting hole 31b should also be elliptical. This ensures that the reversing tube 32 is not easily loosened when inserted into the mounting hole 31b, and provides a tighter connection, thereby preventing the reaction reagents from leaking out of the gap between the two, thereby improving the reaction efficiency and detection accuracy of the reaction device 100.
[0040] Furthermore, the switching structure 30 also includes a sealing ring 33, which is made of a silicone material, preferably self-lubricating silicone. The side wall of the mounting hole 31b is recessed to form a mounting slot 31c, which extends in a circular shape. The sealing ring 33 is partially embedded in the mounting slot 31c. The reversing tube 32 is inserted into the mounting hole 31b, and the sealing ring 33 abuts the reversing tube 32 and the connecting seat 31. This makes the connection between the connecting seat 31 and the reversing tube 32 tighter, and because the sealing ring 33 is made of self-lubricating silicone, the user can rotate the reversing tube 32 more easily and smoothly.
[0041] Furthermore, the switching structure 30 further includes a rotating handle 34, which is connected to the upper portion of the reversing tube 32. In the present application, the rotating handle 34 is provided to facilitate the user to operate the reversing tube 32.
[0042] Please see again Figure 4 After installation, the second tube body 21 is located above the connecting seat 31, and the second connecting tube 22 is tilted toward the connecting seat 31. This arrangement allows the reaction reagents in the second sealed cavity 20a to flow naturally into the first sealed cavity 10a under the action of gravity, making operation simpler.
[0043] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.
Claims
1. A reaction device, characterized in that: include: A first reaction tube is provided with a first sealed cavity and a first connecting channel communicating with the first sealed cavity; A second reaction tube is provided with a second sealed cavity and a second connecting channel communicating with the second sealed cavity; The switching structure includes a connecting seat and a reversing tube, wherein the first reaction tube and the second reaction tube are connected to opposite sides of the connecting seat and are arranged opposite to each other, and the reversing tube is connected to the connecting seat and has a first position and a second position relative to the connecting seat; In the first position, the reversing tube isolates the first connecting channel from the second connecting channel; In the second position, the reversing tube connects the first connecting channel and the second connecting channel.
2. The reaction device according to claim 1, characterized in that The connecting seat is formed with a liquid passage and a mounting hole communicating with the liquid passage; The reversing tube is rotatably inserted into the mounting hole and can rotate relative to the connecting seat between the first position and the second position, so that the liquid passage connects or separates the first sealed cavity and the second sealed cavity.
3. The reaction device according to claim 2, characterized in that The cross section of the reversing tube is gradually reduced from the middle thereof toward both sides.
4. The reaction device according to claim 2, characterized in that The switching structure further includes a sealing ring. The reversing tube is inserted into the mounting hole. The sealing ring abuts against the reversing tube and the connecting seat.
5. The reaction device according to claim 4, characterized in that The side wall of the mounting hole is recessed to form a mounting slot, and a portion of the sealing ring is embedded in the mounting slot.
6. The reaction device according to claim 4, characterized in that The sealing ring is made of self-lubricating silicone.
7. The reaction device according to claim 4, characterized in that The switching structure further includes a rotating handle, which is connected to the upper portion of the reversing tube.
8. The reaction device according to any one of claims 2 to 7, characterized in that The first reaction tube includes a first tube body, a first connecting tube, and a first sealing cover. The first sealing cover covers the first tube body to form the first sealed cavity. The first connecting tube is connected to the first tube body to form the first connecting channel. The second reaction tube includes a second tube body, a second connecting tube, and a second sealing cover. The second sealing cover covers the second tube body to form the second sealed cavity. The second connecting tube is connected to the second tube body to form the second connecting channel. The first connecting pipe and the second connecting pipe are respectively plugged into opposite sides of the liquid passage.
9. The reaction device according to claim 8, characterized in that The second tube body is located above the connecting seat, and the second connecting tube is inclined toward one side of the connecting seat.