Embryo staining operation vessel

Abstract

The utility model discloses an embryo staining operation dish which comprises a base, a hollow structure is arranged in the middle of the base, a transparent piece is embedded in the hollow structure, an operation dish body is arranged on the transparent piece in an overlapping mode, and the base and the operation dish body are connected in a matched mode through a limiting mechanism. Six recessed grooves are formed in the upper surface of the operation dish body, the vertical section of each groove is of a trapezoidal structure with the wide upper portion and the narrow lower portion, an upper cover is connected to the upper portion of the operation dish body in a clamped mode, the grooves are designed to be of the trapezoidal structures, embryos can be effectively fixed, the embryos are prevented from being lost in the operation processes of culturing, washing, dyeing and the like, and the operation dish is convenient to use. The operation dish is simple in structure and convenient to use, embryo concentration is facilitated, embryo loss is avoided, the position of an embryo can be found more easily under a microscope, meanwhile, the base and the operation dish body are designed, the base is used for protecting the operation dish body, the situation that the operation dish body is lost in use is effectively reduced, and the service life is relatively prolonged.

Description

Technical Field

[0001] The utility model relates to the field of biological experiments, in particular to an embryo staining operation dish. Background Art

[0002] Immunofluorescence staining is a commonly used method in early embryonic development research. By labeling specific antigenic proteins, it can reveal the expression patterns, distribution, and dynamic changes of proteins in the embryo, providing key information for exploring the regulatory mechanisms of embryonic development. However, traditional immunofluorescence staining techniques face some challenges when staining embryos.

[0003] First, due to the preciousness of human embryo samples, immunofluorescence staining must ensure a high success rate to avoid embryos failing to stain. This requires not only superb experimental skills but also reliable experimental methods and tools.

[0004] Secondly, embryo loss is a common problem during culture, washing, staining, and other operations. Traditional culture dish designs often fail to properly secure embryos, leading to easy displacement or even loss of embryos during operation, seriously affecting the accuracy and reliability of the experiment.

[0005] How to invent an embryo staining operation dish to improve these problems has become an urgent problem to be solved by those skilled in the art. Utility Model Content

[0006] In order to make up for the above shortcomings, the utility model provides an embryo staining operation dish, which aims to improve the problem that traditional culture dish design often cannot fix embryos well, resulting in easy displacement or even loss of embryos during operation, affecting the accuracy and reliability of the experiment.

[0007] The utility model is implemented as follows: an embryo staining operation dish comprises a base, a hollow structure is provided in the middle of the base, a transparent piece is embedded in the hollow structure, an operation dish body is placed on the transparent piece, the base and the operation dish body are cooperatively connected by a limiting mechanism, six sunken grooves are opened on the upper surface of the operation dish body, and the vertical section of each groove is a trapezoidal structure that is wide at the top and narrow at the bottom. An upper cover is snap-fitted and connected to the upper part of the operation dish body.

[0008] In a preferred technical solution of the present invention, the transparent piece and the operating dish body are entirely made of optical glass.

[0009] In a preferred technical solution of the present invention, the six grooves are centrally arranged in the middle of the entire upper surface of the operating dish body and are distributed in a rectangular array.

[0010] In a preferred technical solution of the present invention, the six grooves are evenly distributed in a ring shape with the center point of the upper surface of the operating dish body as the center.

[0011] In a preferred technical solution of the present invention, the limiting mechanism includes mounting grooves provided at both ends of the upper surface of the base, a card plate is slidably connected to each mounting groove, one end of each mounting groove is communicated with the hollow structure, one end of each card plate extends into the hollow structure and is inserted into the corresponding card slot, the two card slots are respectively provided on the surfaces at both ends of the operating dish body, and a spring is fixedly connected between one side surface of each card plate and the inner wall of one side of the corresponding mounting groove.

[0012] In a preferred technical solution of the present invention, one end of each of the clamping plates located in the hollow structure is configured as a slope structure.

[0013] In a preferred technical solution of the present invention, a sealing rubber pad is provided on the inner wall of the circumference of the upper cover.

[0014] The beneficial effects of the utility model are as follows: the embryo staining operation dish obtained by the above design can effectively fix the embryo when in use by designing the groove into a trapezoidal structure, and prevent the loss of the embryo during operations such as culture, washing, and staining. It is not only beneficial to the concentration of the embryo and avoid the loss of the embryo, but also easier to find the location of the embryo under a microscope, thereby improving the accuracy and reliability of staining; a series of operations such as 3D culture, fixation, membrane permeabilization, sealing, mixed primary antibody incubation, mixed secondary antibody incubation and DAP I staining of the embryo can be achieved in the same culture dish without transferring the embryo, thereby reducing the use of experimental consumables and reducing experimental costs, and maintaining the three-dimensional shape of the embryo, thereby more realistically reflecting the state of the embryo in the body; and the utility model is designed to be composed of two parts, a base and an operation dish body, and the base is used to protect the operation dish body, effectively reducing the loss of the operation dish body during use, and relatively improving the service life. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 This is a schematic three-dimensional diagram of the overall structure provided by an embodiment of the present utility model;

[0017] Figure 2 A schematic three-dimensional diagram of the overall separation structure provided by an embodiment of the present utility model;

[0018] Figure 3 A schematic three-dimensional diagram of the overall cross-sectional structure of the operating dish body provided in an embodiment of the present invention;

[0019] Figure 4 A schematic three-dimensional diagram of the overall structure of the base provided in an embodiment of the present utility model;

[0020] Figure 5 This is a schematic three-dimensional diagram of the overall structure of another embodiment of the operating dish body provided by the embodiment of the utility model.

[0021] In the figure: 1-base; 2-operating dish body; 3-limiting mechanism; 4-upper cover; 101-transparent sheet; 201-groove; 202-card slot; 301-installation slot; 302-card plate; 303-spring. DETAILED DESCRIPTION

[0022] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0023] Example 1

[0024] See also Figures 1 to 4 The utility model provides a technical solution: an embryo staining operation dish, comprising a base 1, a hollow structure is provided in the middle of the base 1, a transparent sheet 101 is embedded in the hollow structure, an operation dish body 2 is placed on the transparent sheet 101, the base 1 and the operation dish body 2 are connected by a limiting mechanism 3, six sunken grooves 201 are provided on the upper surface of the operation dish body 2, and the vertical section of each groove 201 is a trapezoidal structure that is wide at the top and narrow at the bottom, and an upper cover 4 is snap-fitted and connected to the top of the operation dish body 2.

[0025] It should be noted that the bottom area of ​​each groove 201 is half the area of ​​the top opening. This not only helps to centralize the embryo, preventing loss, but also conserves culture medium, antibodies, and other reagents, making it easier to locate the embryo during microscopic observation and photography. Furthermore, after the operating dish body 2 is installed in the hollow structure, its bottom surface fits tightly against the top surface of the transparent sheet 101, preventing gaps from affecting observation and photography.

[0026] See also Figure 2 and Figure 4 The transparent piece 101 and the operating dish body 2 are made of optical glass.

[0027] Optical glass has high light transmittance, making it easy to observe the dyeing effect. At the same time, its chemical stability ensures a clean and safe experimental environment, preventing chemical reactions between the material and experimental reagents from affecting experimental results. The use of optical glass improves observation effects, ensures the accuracy of experimental results, and maintains a clean and safe experimental environment.

[0028] See also Figure 3 The six grooves 201 are centrally arranged in the middle of the upper surface of the operating dish body 2 and are distributed in a rectangular array.

[0029] The rectangular array distribution makes each groove 201 have a relatively independent growth environment, is convenient for the experimenter to locate and operate quickly simultaneously.The groove 201 of trapezoidal structure helps embryo concentration, avoids embryo loss, and keeps the three-dimensional form of embryo simultaneously.All grooves 201 are concentrated in the middle part of the operation dish body 2 overall, thereby avoids the situation that the groove 201 that is positioned at two ends can take incomplete picture when using inverted microscope to observe and shoot.This design improves stability and the efficiency of embryo culture, reduces embryo loss rate, and is convenient for the experimenter to observe and operate simultaneously.

[0030] See also Figure 3 and Figure 4 The limiting mechanism 3 includes mounting grooves 301 provided at both ends of the upper surface of the base 1, and a clamping plate 302 is slidably connected to each mounting groove 301. One end of each mounting groove 301 is connected to the hollow structure, and one end of each clamping plate 302 extends into the hollow structure and is inserted into the corresponding clamping groove 202. The two clamping grooves 202 are respectively provided on the surfaces of both ends of the operating dish body 2, and a spring 303 is fixedly connected between the surface of one side of each clamping plate 302 and the inner wall of one side of the corresponding mounting groove 301.

[0031] The limiting mechanism securely fixes the operating dish body 2 by cooperating between the clamping plate 302 and the clamping slot 202. The spring 303 provides a reset force for the clamping plate 302, facilitating the installation and removal of the operating dish body 2. This ensures a stable connection between the base 1 and the operating dish body 2.

[0032] Furthermore, one end of each clamping plate 302 located in the hollow structure is configured as a slope structure.

[0033] When the operating dish body 2 is placed into the hollow structure, its two ends are guided to push the clamping plate 302 to the sides by the inclined surface structure, and then the clamping plate 302 is automatically reset under the drive of the spring 303 and directly inserted into the corresponding clamping slot 202. The inclined surface structure simplifies the installation process and improves the operation efficiency.

[0034] See also Figure 1 and Figure 2, sealing pads are provided on the inner walls of the four circles around the upper cover.

[0035] The sealing rubber gasket is used to ensure a tight fit between the upper cover 4 and the operating dish body 2, effectively preventing liquid evaporation or external contamination during the experiment, thereby improving the cleanliness of the experimental environment and ensuring the accuracy of the experimental results.

[0036] Example 2

[0037] See also Figures 1 to 5 The utility model provides a technical solution: an embryo staining operation dish, comprising a base 1, a hollow structure is provided in the middle of the base 1, a transparent sheet 101 is embedded in the hollow structure, an operation dish body 2 is placed on the transparent sheet 101, the base 1 and the operation dish body 2 are connected by a limiting mechanism 3, six sunken grooves 201 are provided on the upper surface of the operation dish body 2, and the vertical section of each groove 201 is a trapezoidal structure that is wide at the top and narrow at the bottom, and an upper cover 4 is snap-fitted and connected to the top of the operation dish body 2.

[0038] It should be noted that the bottom area of ​​each groove 201 is half the area of ​​the top opening. This not only helps to centralize the embryo, preventing loss, but also conserves culture medium, antibodies, and other reagents, making it easier to locate the embryo during microscopic observation and photography. Furthermore, after the operating dish body 2 is installed in the hollow structure, its bottom surface fits tightly against the top surface of the transparent sheet 101, preventing gaps from affecting observation and photography.

[0039] See also Figure 2 and Figure 4 The transparent piece 101 and the operating dish body 2 are made of optical glass.

[0040] Optical glass has high light transmittance, making it easy to observe the dyeing effect. At the same time, its chemical stability ensures a clean and safe experimental environment, preventing chemical reactions between the material and experimental reagents from affecting experimental results. The use of optical glass improves observation effects, ensures the accuracy of experimental results, and maintains a clean and safe experimental environment.

[0041] See also Figure 5 The six grooves 201 are evenly distributed in a circular shape with the center point of the upper surface of the operating dish body 2 as the center.

[0042] If the culture process involves rotation or centrifugation, the evenly distributed circular grooves can better accommodate such operations, ensuring that each embryo is subjected to a more uniform centrifugal force, helping to maintain embryo stability. The circular distribution also provides a more diverse observation angle, allowing researchers to observe the growth of embryos from different perspectives, helping to obtain more comprehensive information.

[0043] See also Figure 3 and Figure 4 The limiting mechanism 3 includes mounting grooves 301 provided at both ends of the upper surface of the base 1, and a clamping plate 302 is slidably connected to each mounting groove 301. One end of each mounting groove 301 is connected to the hollow structure, and one end of each clamping plate 302 extends into the hollow structure and is inserted into the corresponding clamping groove 202. The two clamping grooves 202 are respectively provided on the surfaces of both ends of the operating dish body 2, and a spring 303 is fixedly connected between the surface of one side of each clamping plate 302 and the inner wall of one side of the corresponding mounting groove 301.

[0044] The limiting mechanism securely fixes the operating dish body 2 by cooperating between the clamping plate 302 and the clamping slot 202. The spring 303 provides a reset force for the clamping plate 302, facilitating the installation and removal of the operating dish body 2. This ensures a stable connection between the base 1 and the operating dish body 2.

[0045] Furthermore, one end of each clamping plate 302 located in the hollow structure is configured as a slope structure.

[0046] When the operating dish body 2 is placed into the hollow structure, its two ends are guided to push the clamping plate 302 to the sides by the inclined surface structure, and then the clamping plate 302 is automatically reset under the drive of the spring 303 and directly inserted into the corresponding clamping slot 202. The inclined surface structure simplifies the installation process and improves the operation efficiency.

[0047] See also Figure 1 and Figure 2 , sealing pads are provided on the inner walls of the four circles around the upper cover.

[0048] The sealing rubber gasket is used to ensure a tight fit between the upper cover 4 and the operating dish body 2, effectively preventing liquid evaporation or external contamination during the experiment, thereby improving the cleanliness of the experimental environment and ensuring the accuracy of the experimental results.

[0049] Working principle: First, embryos are placed in the six grooves 201 to perform experimental operations. The trapezoidal grooves 201 provide a centralized growth environment for the embryos, preventing embryo loss while maintaining the three-dimensional morphology of the embryos. When the experiment is completed and the embryos in each groove 201 need to be observed and photographed, the base 1 and the operation dish body 2 are placed as a slide on an inverted microscope. Because the transparent glass 101 and the operation dish body 2 are all made of optical glass, high light transmittance and chemical stability are guaranteed, improving the observation effect and the cleanliness of the experimental environment. At the same time, the base 1 and the operation dish body 2 are designed as two independent parts connected by a limiting mechanism 3. Different operation dish bodies 2 and base 1 can be selected for installation according to the specific experimental operation needs. The rectangular array of grooves 201 can fully utilize the space of the entire operation dish body 2, while the annular evenly distributed grooves 201 can adapt to the situation involving rotation or centrifugation during the culture process, ensuring that the centrifugal force exerted on the embryos in each groove 201 is consistent, thereby reducing limitations in use.

[0050] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. An embryo staining operation dish, characterized in that: The utility model comprises a base, a hollow structure is provided in the middle of the base, a transparent piece is embedded in the hollow structure, an operating dish body is placed on the transparent piece, the base and the operating dish body are connected by a limiting mechanism, six sunken grooves are provided on the upper surface of the operating dish body, and the vertical section of each groove is a trapezoidal structure that is wide at the top and narrow at the bottom. An upper cover is snap-fitted to the top of the operating dish body.

2. The embryo staining operation dish according to claim 1, wherein: The transparent piece and the operating dish body are made entirely of optical glass.

3. The embryo staining operation dish according to claim 1, wherein: The six grooves are centrally arranged in the middle of the upper surface of the operating dish body and are distributed in a rectangular array.

4. The embryo staining operation dish according to claim 1, wherein: The six grooves are evenly distributed in a ring shape with the center point of the upper surface of the operating dish body as the center.

5. The embryo staining operation dish according to claim 1, wherein: The limiting mechanism includes mounting grooves provided at both ends of the upper surface of the base, a card plate is slidably connected to each mounting groove, one end of each mounting groove is communicated with the hollow structure, one end of each card plate extends into the hollow structure and is inserted into the corresponding card slot, the two card slots are respectively provided on the surfaces at both ends of the operating dish body, and a spring is fixedly connected between one side surface of each card plate and the inner wall of one side of the corresponding mounting groove.

6. The embryo staining operation dish according to claim 5, wherein: One end of each of the clamping plates located in the hollow structure is configured as an inclined structure.

7. The embryo staining operation dish according to claim 1, wherein: Sealing rubber pads are provided on the inner walls of the upper cover circumference.

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