Sample pretreatment device based on magnetic nanoparticles

Abstract

The utility model provides a sample pretreatment device based on magnetic nanoparticles, and relates to the technical field of sample pretreatment. The sample pretreatment device based on the magnetic nanoparticles comprises a sample placing pore plate used for placing the magnetic nanoparticles and a to-be-treated sample or a cleaning reagent; the sample mixing module is used for mixing or cleaning the sample to be treated and the magnetic nanoparticles; the height control module is used for connecting the sample placing pore plate and the sample mixing module and is used for adjusting the height of the sample placing pore plate, so that the magnetic nanoparticles adsorbing the impurities in the to-be-treated sample are adsorbed to the bottommost layer of the sample placing pore plate; the regeneration module is arranged above the sample placing pore plate, is not connected with the sample placing pore plate and is used for regenerating the magnetic nanoparticles; the device frame is used for fixing the sample mixing module and the regeneration module. Through highly integrated modular design, the sample pretreatment process is simplified, and the sample pretreatment efficiency is improved.

Description

Technical Field

[0001] The present application relates to the technical field of sample pretreatment, for example, to a sample pretreatment device based on magnetic nanoparticles. Background Art

[0002] Sample pretreatment, also known as sample pretreatment, involves a series of steps to decompose the sample and quantitatively transfer the analyte into solution, facilitating subsequent analysis and measurement. This step applies to both inorganic and organic samples, but the treatment methods differ. Sample pretreatment involves enriching the target substance in the sample and removing interfering substances to improve the accuracy and sensitivity of the target substance in subsequent analysis.

[0003] In related technologies, magnetic nanoparticles are used for sample pretreatment. However, in order to completely process the sample, the experimenter needs to move the sample multiple times and use different devices to remove interferences and extract the target substance. The overall pretreatment process is very cumbersome, resulting in low processing efficiency. Summary of the Invention

[0004] The present application aims to provide a sample pretreatment device based on magnetic nanoparticles.

[0005] According to one aspect of the present application, a sample pretreatment device based on magnetic nanoparticles is proposed, comprising: a sample placement plate for placing magnetic nanoparticles and samples to be processed or cleaning reagents; a sample mixing module for mixing or cleaning samples to be processed and magnetic nanoparticles; a height control module for connecting the sample placement plate and the sample mixing module, and for adjusting the height of the sample placement plate, so that the magnetic nanoparticles that have adsorbed impurities in the sample to be processed are adsorbed to the bottom layer of the sample placement plate; a regeneration module, which is arranged above the sample placement plate and is not connected to the sample placement plate, and is used to regenerate the magnetic nanoparticles; and a device frame for fixing the sample mixing module and the regeneration module.

[0006] According to some embodiments, the sample mixing module includes: a vortexer, disposed below the height control module, for vortexing the sample to be processed and the magnetic nanoparticles to maximize the contact area between the magnetic nanoparticles and impurities.

[0007] According to some embodiments, the height control module includes: an electromagnetic plate, disposed above the vortexer, for adsorbing the magnetic nanoparticles adsorbed with impurities to the bottom layer of the sample placement well plate when powered on.

[0008] According to some embodiments, the height control module also includes: a support frame, arranged between the electromagnetic plate and the sample placement hole plate, for supporting the sample placement hole plate, the support frame includes a plurality of support columns, and the plurality of support columns are support columns with adjustable support height to adjust the magnetic strength of the electromagnetic plate.

[0009] According to some embodiments, the support heights of the plurality of support columns are adjusted by rotation.

[0010] According to some embodiments, the sample mixing module includes: a temperature integration submodule, which is arranged between the vortexer and the electromagnetic plate, and is used to provide corresponding reaction temperatures according to different experimental requirements.

[0011] According to some embodiments, the electromagnetic plate and the sample placement hole plate are provided with the same number of clips in corresponding positions.

[0012] According to some embodiments, the electromagnetic plate and the vortexer are provided with the same number of clips in corresponding positions.

[0013] According to some embodiments, the regeneration module includes: a plurality of magnetic columns for absorbing the cleaned magnetic nanoparticles, wherein one end of the plurality of magnetic columns is fixed on the device frame; a disinfection submodule, fixed on the device frame, for disinfecting the magnetic nanoparticles; and a drying submodule, corresponding to the height of the other end of the plurality of magnetic columns, for drying the magnetic nanoparticles.

[0014] According to some embodiments, the plurality of magnetic poles are arranged in a matrix; wherein the number of magnetic poles in each row is the same, and the positions of the magnetic poles in each column are aligned with each other.

[0015] It should be understood that the foregoing general description and the following detailed description are merely illustrative and are not restrictive of the present application.

[0016] Beneficial effects:

[0017] Through the above-mentioned embodiments provided in this application, the sample pre-treatment process is effectively simplified through a highly integrated modular design. Specifically, the sample placement orifice plate, sample mixing module, height control module and regeneration module in the device cooperate with each other to form a complete automated processing system. The experimenter only needs to place the magnetic nanoparticles and the sample to be processed or the cleaning reagent into the sample placement orifice plate, and the device can complete a series of operations such as mixing, cleaning, impurity adsorption, and magnetic nanoparticle regeneration. The number of operations and labor intensity of the experimenter are greatly reduced, and the efficiency and accuracy of sample pre-treatment are also improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without exceeding the scope of protection required by this application.

[0019] Figure 1 A schematic structural diagram of a sample pretreatment device based on magnetic nanoparticles provided in an embodiment of the present application;

[0020] Figure 2 A schematic diagram of the structure of a sample mixing module provided in an embodiment of the present application;

[0021] Figure 3 A schematic diagram of the structure of the height control module provided in an embodiment of the present application;

[0022] Figure 4 This is a schematic diagram of the structure of the regeneration module provided in an embodiment of the present application. DETAILED DESCRIPTION

[0023] Example embodiments will now be described more fully with reference to the accompanying drawings. However, example embodiments can be embodied in many forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete and will fully convey the concepts of the example embodiments to those skilled in the art. Like reference numerals in the drawings represent like or similar parts, and thus repetitive description thereof will be omitted.

[0024] In addition, described feature, structure or characteristic can be combined in one or more embodiments in any suitable manner.In the following description, many specific details are provided so as to provide a full understanding of the embodiments of the present application. However, it will be appreciated by those skilled in the art that the technical scheme of the present application can be put into practice without one or more of the specific details, or other methods, components, devices, steps etc. can be adopted. In other cases, known methods, devices, implementations or operations are not shown or described in detail to avoid blurring the various aspects of the application.

[0025] The block diagrams shown in the accompanying drawings are merely functional entities and do not necessarily correspond to physically separate entities. That is, these functional entities may be implemented in software, in one or more hardware modules or integrated circuits, or in different networks and / or processor devices and / or microcontroller devices.

[0026] The flowcharts shown in the accompanying drawings are for illustrative purposes only and do not necessarily include all contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may be decomposed, while others may be combined or partially combined. Therefore, the actual execution order may vary depending on the actual situation.

[0027] It should be understood that although the terms first, second, third, etc. may be used herein to describe various components, these components should not be limited by these terms. These terms are used to distinguish one component from another. Thus, the first component discussed below could be referred to as the second component without departing from the teachings of the present invention. As used herein, the term "and / or" includes any one and all combinations of one or more of the associated listed items.

[0028] In the present application, the sample pretreatment device as a whole is a multifunctional integrated device for sample impurity removal, temperature control, electromagnetic control, magnetic bead cleaning and ultraviolet sterilization. The device is provided with an overall device frame for fixing modules with different functions. These modules may include a sample placement plate, a sample mixing module, a height control module and a regeneration module. These modules may also include more specific functional components. By using the device of the present application, the tedious operation of multiple equipment changes and sample movement by relevant experimenters can be avoided, thereby improving the processing efficiency of sample pretreatment.

[0029] For specific implementation methods, please refer to the following embodiments.

[0030] Figure 1 This is a schematic diagram of the structure of a sample pre-processing device based on magnetic nanoparticles provided in an embodiment of the present application. Figure 1 As shown, the device includes: a sample placement plate 1, a sample mixing module 2, a height control module 3, a regeneration module 4 and a device frame 5.

[0031] A sample placement well plate 1 is used to place magnetic nanoparticles and samples to be processed or cleaning reagents; a sample mixing module 2 is used to mix or clean samples to be processed and magnetic nanoparticles; a height control module 3 is used to connect the sample placement well plate 1 and the sample mixing module 2, and to adjust the height of the sample placement well plate 1, so that the magnetic nanoparticles that have adsorbed impurities in the sample to be processed are adsorbed to the bottom layer of the sample placement well plate 1; a regeneration module 4 is arranged above the sample placement well plate 1 and is not connected to the sample placement well plate 1, and is used to regenerate the magnetic nanoparticles; and a device frame 5 is used to fix the sample mixing module 2 and the regeneration module 4.

[0032] In the present application, the surface of the magnetic nanoparticles is customized according to different experimental requirements, and the cleaning reagent is selected according to the cleaning requirements and the properties of the magnetic nanoparticles.

[0033] The sample placement plate 1 in the present application may be a 96-well plate with sample tubes placed thereon, and the experimenter may place the magnetic nanoparticles, the sample to be processed / cleaning reagent into the sample placement plate 1. The sample mixing module 2 may be an integrated module for mixing the magnetic nanoparticles and the sample to be processed, or the magnetic nanoparticles and the cleaning reagent.

[0034] Since the sample placement plate 1 comprises a plate and sample tubes, it needs to be supported, leaving at least enough height for the sample tubes themselves. Therefore, the height control module 3 is a support-type module that adjusts the height of the sample placement plate 1. In some implementations, to enhance the mixing of magnetic nanoparticles with the sample to be processed / washing reagent and the separation of the magnetic nanoparticles, the sample mixing module 2 may also be a module that integrates a support-type component with components that enhance mixing and separation capabilities.

[0035] The sample placement well plate 1 can be placed on the height control module 3 , and a soup mixing module is provided below the height control module 3 .

[0036] The operation of the sample mixing module 2 can enable the magnetic nanoparticles to fully absorb impurities in the sample to be processed. In order to make the magnetic nanoparticles reusable, the magnetic nanoparticles that have absorbed impurities need to be cleaned, that is, the experimenter puts the cleaning reagent into the sample placement well plate 1, and the sample mixing module 2 works to fully clean the magnetic nanoparticles. The cleaned magnetic nanoparticles need to be extracted, and the regeneration module 4 is a module that adsorbs the cleaned magnetic nanoparticles to achieve regeneration. The regeneration module 4 is arranged above the sample placement well plate 1 and is not connected to the sample placement well plate 1, so that the magnetic nanoparticles can be moved from each hole of the sample placement well plate 1 to the regeneration module 4.

[0037] Since the sample pretreatment device based on magnetic nanoparticles of the present application is a multifunctional integrated device, different functions correspond to different modules. In order to integrate the various modules into a system, a device frame 5 is provided to install the modules.

[0038] This application effectively simplifies the sample pre-treatment process through a highly integrated modular design. Specifically, the sample placement plate, sample mixing module, height control module, and regeneration module in the device cooperate with each other to form a complete automated processing system. The experimenter only needs to place the magnetic nanoparticles and the sample to be processed or the cleaning reagent into the sample placement plate, and the device can complete a series of operations such as mixing, cleaning, impurity adsorption, and magnetic nanoparticle regeneration. This greatly reduces the number of operations and labor intensity of the experimenter, and also improves the efficiency and accuracy of sample pre-treatment.

[0039] According to some embodiments, reference Figure 2This is a structural diagram of the sample mixing module provided in an embodiment of the present application. The sample mixing module 2 includes: a vortex 21, which is arranged below the height control module 3 and is used to vortex the sample to be processed and the magnetic nanoparticles to maximize the contact area between the magnetic nanoparticles and impurities.

[0040] In the present application, the vortex vortexer 21 is a device that utilizes the electromagnetic field of a circuit element to generate eccentric rotation and oscillation, or a motor drives an eccentric component (such as an eccentric ball bearing) to generate eccentric rotation, thereby achieving sample mixing.

[0041] This application achieves efficient vortex oscillation of the sample and magnetic nanoparticles by introducing a vortex oscillator and placing it below the height control module. The intense vortex motion generated by the vortex oscillation can greatly increase the contact area and contact frequency between the magnetic nanoparticles and impurities in the sample, thereby significantly increasing the probability of impurities being captured by the magnetic nanoparticles, laying a solid foundation for subsequent magnetic separation and sample purification steps.

[0042] According to some embodiments, reference Figure 3 This is a structural diagram of the height control module provided in an embodiment of the present application. The height control module 3 includes: an electromagnetic plate 31, which is arranged above the vortex meter 21 and is used to adsorb magnetic nanoparticles adsorbed with impurities to the bottom layer of the sample placement well plate 1 when power is turned on.

[0043] In this application, the electromagnetic plate 31 can be made of high-performance electromagnetic materials to ensure that it can generate sufficient magnetic field strength when powered, thereby effectively adsorbing magnetic nanoparticles. The surface of the electromagnetic plate 31 should be specially treated to improve its wear resistance, corrosion resistance and service life.

[0044] In some implementations, the shape and size of the electromagnetic plate 31 should match the sample placement well plate 1 to ensure that all sample wells on the well plate are fully covered during the adsorption process. The interior of the electromagnetic plate 31 can be designed with a reasonable coil layout and heat dissipation structure to ensure stable operation when powered for long periods of time.

[0045] The electromagnetic plate 31 is disposed above the vortexer 21. In some implementations, the electromagnetic plate 31 may be at a certain height from the sample placement well plate 1 to ensure that the magnetic nanoparticles can be accurately adsorbed to the bottom layer of the well plate when powered on.

[0046] In some implementations, the height control module 3 can be externally connected to a precise power control device, enabling precise power control of the electromagnetic plate 31 based on experimental requirements or external control by the experimenter. After the vortex 21 completes the vortexing of the sample and magnetic nanoparticles, the electromagnetic plate 31 can be powered on. This power-on electromagnetic plate 31 generates a strong magnetic field, precisely adsorbing the impurity-laden magnetic nanoparticles to the bottom layer of the sample placement well plate 1.

[0047] This application uses the powerful magnetic field generated by the electromagnetic plate when it is powered on to quickly and effectively separate the magnetic nanoparticles adsorbed with impurities from the mixed liquid and accurately adsorb them to the bottom layer of the sample placement well plate. This process not only reduces the residual impurities in the mixed liquid, but also improves the efficiency and accuracy of impurity removal, providing purer samples for subsequent experimental steps. The collaborative work of the height control module and the vortex instrument makes the sample pre-treatment process more automated and efficient. After the vortex instrument completes the mixing and oscillation, the electromagnetic plate is immediately powered on for impurity adsorption, and the sample can be quickly purified without human intervention. This design not only simplifies the operating process, but also reduces the workload and operation time of the experimenter, and improves the overall experimental efficiency.

[0048] According to some embodiments, reference Figure 3 This is a structural schematic diagram of the height control module provided in an embodiment of the present application. The height control module 3 also includes: a support frame 32, which is arranged between the electromagnetic plate 31 and the sample placement well plate 1, and is used to support the sample placement well plate 1. The support frame 32 includes a plurality of support columns, and the plurality of support columns are support columns with adjustable support heights to adjust the magnetic strength of the electromagnetic plate 31.

[0049] In the present application, the support column (not shown in the figure) is made of high-strength, corrosion-resistant materials, such as stainless steel or aluminum alloy, to ensure stable support performance during long-term use. The shape and size of the support column should be designed according to actual use requirements to ensure sufficient support strength and stability. Each support column is provided with an adjustment mechanism. In some implementations, the adjustment mechanism can be threaded adjustment, air pressure adjustment or hydraulic adjustment, etc., to achieve precise adjustment of the support height. The adjustment mechanism has a locking component that can lock the support height at the desired height position to prevent accidental displacement during use.

[0050] The support frame 32 is composed of multiple support columns and connectors to form a stable support frame. The connectors should be made of high-strength, corrosion-resistant materials and designed with a reasonable connection method to ensure the overall stability and durability of the support frame 32.

[0051] By adjusting the height of the support column, the distance between the electromagnetic plate 31 and the sample placement hole plate 1 can be changed, thereby adjusting the range of magnetic action of the electromagnetic plate 31 on the magnetic nanoparticles. The adjustment setting of the support height can be accurate to the millimeter level to ensure precise control of the magnetic strength. When the height of the support column is lowered, the distance between the electromagnetic plate 31 and the sample placement hole plate 1 is reduced, the magnetic lines of force are more dense, and the magnetic strength is enhanced, which is conducive to adsorbing more magnetic nanoparticles onto the sample placement hole plate 1. On the contrary, when the height of the support column is increased, the distance between the electromagnetic plate 31 and the sample placement hole plate 1 is increased, the magnetic lines of force become sparse, and the magnetic strength is weakened, which can reduce the adsorption force on the magnetic nanoparticles and facilitate subsequent cleaning and separation operations.

[0052] By adjusting the height of the support column, the present application can accurately control the distance between the electromagnetic plate and the sample placement hole plate, and then adjust the magnetic strength generated by the electromagnetic plate. This precise adjustment capability enables experimenters to flexibly adjust the magnetic strength according to different experimental requirements to achieve the best impurity separation effect. By accurately adjusting the magnetic strength, magnetic nanoparticles adsorbed with impurities can be more effectively separated from the mixed liquid and adsorbed to the bottom layer of the sample placement hole plate. This not only reduces the residual impurities in the sample, but also improves the efficiency and accuracy of impurity separation. The height adjustment function of the support frame enables the electromagnetic plate to fit more closely to the sample placement hole plate, thereby reducing the leakage and loss of magnetic lines of force. This close fit not only improves the efficiency of the magnetic action, but also helps to improve the overall effect of sample processing, making the final sample purer and more reliable.

[0053] According to some embodiments, the support heights of the plurality of support columns are adjusted by rotation.

[0054] In this application, each support column can be composed of three parts: a column body, a rotary adjustment device, and a base. The column body is made of a high-strength, lightweight material (such as aluminum alloy or carbon fiber) to ensure support strength while reducing weight. The column body surface can be designed with scale lines to facilitate the experimenter to intuitively understand the current support height.

[0055] The rotation adjustment device is located between the column and the base, and can achieve rotational adjustment through a threaded connection or a ratchet mechanism. In some implementations, if the connection is threaded, the column can be internally threaded, and the base can have matching external threads, and the column can be raised and lowered by rotating the column. In other implementations, if the connection is a ratchet mechanism, the column or base can be equipped with a ratchet, and the ratchet handle can be rotated to achieve raising and lowering. The ratchet also has a self-locking function to prevent the support column from automatically lowering when loaded.

[0056] This application uses a rotary adjustment method to allow experimenters to easily and precisely adjust the height of each support column. Compared to other adjustment methods, rotary adjustment is simpler and faster. Experimenters can quickly adjust the height by simply rotating the adjustment component on the support column. This operation method not only saves time and effort, but also improves the user experience, making the adjustment process smoother and more intuitive.

[0057] According to some embodiments, reference Figure 2 This is a structural diagram of the sample mixing module provided in an embodiment of the present application. The sample mixing module 2 includes: a temperature integration submodule 22, which is arranged between the vortex meter 21 and the electromagnetic plate 31, and is used to provide corresponding reaction temperatures according to different experimental requirements.

[0058] In this application, the temperature integration submodule 22 is disposed between the vortexer 21 and the electromagnetic plate 31. This arrangement ensures uniform temperature control of the sample during mixing while avoiding direct interference with the electromagnetic plate 31, thereby ensuring the accuracy and stability of the experiment. The temperature integration submodule 22 has a relatively wide temperature control range, capable of providing a variety of reaction temperatures from low to high temperatures according to different experimental requirements.

[0059] In some implementations, an efficient heating and cooling system can be installed within the module to quickly and stably heat or cool the sample to the desired reaction temperature. The heating system can use components such as resistance wire or heating plates, while the cooling system can include semiconductor refrigeration chips, air cooling, or water cooling devices. To monitor and control the sample temperature in real time, the temperature integration submodule 22 can have a built-in high-precision temperature sensor that displays real-time temperature data.

[0060] The temperature integration submodule 22 of the present application can provide accurate reaction temperature according to different experimental requirements. This precise temperature control is crucial for many chemical reactions and biological processes and can ensure the accuracy and reliability of experimental results. Under the action of the vortex instrument 21, the sample is subjected to strong shearing and impact forces during the mixing process. The suitable temperature provided by the temperature integration submodule 22 can accelerate the movement and collision of molecules, thereby further improving the mixing efficiency of the sample. The suitable temperature helps to improve the solubility and dispersibility of the sample, so that the components in the sample can be mixed more evenly.

[0061] According to some embodiments, the electromagnetic plate 31 and the sample placement well plate 1 are provided with the same number of clips at corresponding positions.

[0062] In this application, a sample placement plate 1 is provided with sample tubes containing magnetic nanoparticles and samples to be processed or cleaning reagents. Corresponding baffles are provided. The baffles are perpendicularly disposed at the corresponding ends of the plate containing the sample tubes. The height of the baffles is generally higher than the height of the sample tubes. The baffles can be connected to the electromagnetic plate 31.

[0063] The buckle can be a resilient structure, typically made of a high-strength, wear-resistant, and somewhat resilient material, such as stainless spring steel or a special plastic. The buckle can be shaped like a hook, a ring, or other shapes that provide a secure connection and are easily removable, thereby ensuring a stable connection between the electromagnetic plate 31 and the sample placement well plate 1.

[0064] In some implementations, the electromagnetic plate 31 and the sample placement plate 1 are each provided with at least two latches, and the number of these latches on the electromagnetic plate 31 and the sample placement plate 1 is exactly the same. Each latch is located at a corresponding position on the electromagnetic plate 31 and the sample placement plate 1, that is, when a latch is located at a specific position on the electromagnetic plate 31, a corresponding latch is located at the same or a mirror image position on the sample placement plate 1.

[0065] The design of the clips in this application ensures a stable connection between the electromagnetic plate and the sample placement plate. Through the clips of the same number and corresponding positions, the two can be accurately aligned and fit tightly, effectively preventing displacement or shaking during use. The clip connection method simplifies the installation and removal process of the electromagnetic plate and the sample placement plate. Experimenters do not need to use additional tools or complicated operating steps, and can complete the connection or separation through simple clip operations. This not only improves work efficiency, but also reduces the difficulty of operation, allowing ordinary experimenters to easily complete the installation and removal work.

[0066] According to some embodiments, the electromagnetic plate 31 and the vortexer 31 are provided with the same number of clips at corresponding positions.

[0067] In the present application, the buckles on the electromagnetic plate 31 and the vortex meter 21 can be designed into specific geometric shapes, such as hooks, rings or structures with elastic protrusions, to ensure that they can be firmly connected together. The buckle material can be selected from high-strength, corrosion-resistant and elastic materials, such as stainless steel or special alloys, to withstand the stress and wear that may occur during use. The number of buckles can be configured according to the size, weight and connection requirements of the electromagnetic plate 31 and the vortex meter 21 to ensure that the connection between the two is both stable and uniform. The position of the buckle corresponds precisely to the contact surface of the electromagnetic plate 31 and the vortex meter 21 to form a one-to-one connection point.

[0068] In some implementations, to enhance the stability of the connection, the buckle may be equipped with a locking mechanism. Once the buckle is fully inserted into the slot, the locking mechanism automatically activates, securing the buckle in the slot and preventing it from accidentally falling out. To unlock the buckle, the experimenter needs to perform a specific action, such as pressing an unlock button or rotating the buckle, to release the locking force.

[0069] The design of the clips in this application ensures precise positioning between the electromagnetic plate and the vortex meter. Each clip is precisely calculated and positioned so that the two can quickly and accurately find their corresponding positions when connected, avoiding performance degradation caused by misalignment or deviation. The clip connection is not only stable and reliable, but also has a certain buffering and shock-absorbing effect. When the device is subjected to external impact or vibration, the clip can absorb part of the energy, reducing damage to the electromagnetic plate and vortex meter, thereby improving the safety and durability of the equipment.

[0070] According to some embodiments, reference Figure 4 This is a structural schematic diagram of the regeneration module provided in an embodiment of the present application, where the regeneration module 4 includes: a plurality of magnetic columns 41 for adsorbing cleaned magnetic nanoparticles, wherein one end of the plurality of magnetic columns 41 is fixed to the device frame 5; a disinfection submodule 42, fixed to the device frame 5, for disinfecting the magnetic nanoparticles; and a drying submodule 43, wherein the drying submodule 43 corresponds to the height of the other end of the plurality of magnetic columns 41 and is used for drying the magnetic nanoparticles.

[0071] In this application, the magnetic column 41 is made of a magnetic material and has a strong magnetic attraction, which can firmly adsorb the cleaned magnetic nanoparticles. One end of the magnetic column 41 is firmly fixed to the device frame 5 by bolts, slots, or other fixing methods to ensure that it does not move or fall off during the experiment. The number and layout of the magnetic columns 41 are reasonably designed according to the experimental requirements and the capacity of the magnetic nanoparticles to efficiently process the sample.

[0072] The disinfection submodule 42 is fixed on the device frame 5, and is located above or on the side of the magnetic column 41, so as to disinfect the magnetic nanoparticles adsorbed on the magnetic column 41. The disinfection submodule 42 can use ultraviolet disinfection, ozone disinfection or other effective disinfection methods. The specific choice depends on the experimental requirements and the disinfection effect. The disinfection submodule 42 is equipped with sensors and control modules, which can monitor parameters such as temperature, humidity and disinfection time during the disinfection process in real time to ensure the disinfection effect. In some implementations, the disinfection submodule 42 needs to be cleaned once after each sample processing is completed.

[0073] The drying submodule 43 is positioned at the same height as the other ends of the multiple magnetic pins 41 to dry the magnetic nanoparticles adsorbed on the pins 41. Drying submodule 43 can utilize hot air drying, vacuum drying, or other effective drying methods, depending on experimental requirements and drying effect. Drying submodule 43 is equipped with components such as a heating element, a fan, or a vacuum pump, as well as control systems for parameters such as temperature, humidity, and drying time to ensure the stability and efficiency of the drying process.

[0074] According to an exemplary embodiment, after the magnetic nanoparticles are cleaned, the plurality of magnetic posts 41 may be energized to attract the magnetic nanoparticles in the sample placement well plate 1 to the magnetic posts 41. The disinfection submodule 42 and the drying submodule 43 may then be activated to dry and disinfect the magnetic nanoparticles attracted to the magnetic posts 41.

[0075] This application utilizes multiple magnetic columns, allowing the regeneration module to simultaneously process multiple samples or large quantities of magnetic nanoparticles, significantly improving experimental efficiency. The regeneration module integrates the three key steps of adsorption, disinfection, and drying into a coherent automated process. This not only reduces manual labor but also improves experimental accuracy and consistency.

[0076] According to some embodiments, the plurality of magnetic poles 41 are arranged in a matrix; wherein the number of magnetic poles 41 in each row is the same, and the positions of the magnetic poles 41 in each column are aligned with each other.

[0077] In this application, the space on the device frame 5 can be rationally divided into multiple rows and columns, with each row and column maintained at equal distances or adjusted according to actual needs to ensure a uniform distribution of the magnetic posts 41. The rows and columns are divided according to the size and shape of the magnetic posts 41 and the minimum safe distance between adjacent magnetic posts 41 to avoid mutual interference during the adsorption, disinfection, and drying processes. In some implementations, 12 rows and 8 columns of magnetic posts 41 can be arranged.

[0078] The number of magnetic columns 41 in each row remains consistent, facilitating management and operation. The columns 41 are aligned to form a neat array, ensuring uniform and consistent treatment of the magnetic nanoparticles during adsorption, disinfection, and drying. One end of the magnetic column 41 can be securely fastened to the device frame 5 via bolts, slots, magnets, or other reliable fastening methods. The fastening method can be designed to facilitate removal and replacement of the magnetic column 41, allowing for cleaning, disinfection, and maintenance after the experiment.

[0079] This application rationally divides the space on the device rack into multiple rows and columns. The spacing between each row and column is equidistant, or adjusted as needed, to ensure uniform distribution of the magnetic pins. The number of magnetic pins in each row is consistent, facilitating management and operation. The magnetic pins in each column are aligned, forming a neat array layout. This helps ensure uniform and consistent treatment of the magnetic nanoparticles during adsorption, disinfection, and drying.

[0080] The embodiments of the present application are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only intended to help understand the method and core ideas of the present application. At the same time, changes or modifications made by those skilled in the art based on the ideas of the present application, the specific implementation methods, and the scope of application of the present application, all fall within the scope of protection of the present application. In summary, the contents of this specification should not be construed as limiting the present application.

Claims

1. A sample pretreatment device based on magnetic nanoparticles, characterized in that: include: A sample placement well plate, used for placing magnetic nanoparticles and samples to be processed or cleaning reagents; A sample mixing module, used for mixing or washing the sample to be processed and the magnetic nanoparticles; a height control module, configured to connect the sample placement plate and the sample mixing module and to adjust the height of the sample placement plate so that the magnetic nanoparticles adsorbed with impurities in the sample to be processed are adsorbed to the bottom layer of the sample placement plate; a regeneration module, disposed above the sample placement well plate and not connected to the sample placement well plate, for regenerating the magnetic nanoparticles; A device frame is used to fix the sample mixing module and the regeneration module.

2. The device according to claim 1, characterized in that The sample mixing module comprises: The vortexer is disposed below the height control module and is used to vortex the sample to be processed and the magnetic nanoparticles to maximize the contact area between the magnetic nanoparticles and the impurities.

3. The device according to claim 2, characterized in that The height control module includes: The electromagnetic plate is arranged above the vortex instrument and is used for adsorbing the magnetic nanoparticles adsorbed with impurities to the bottom layer of the sample placement well plate when powered on.

4. The device according to claim 3, characterized in that The height control module further includes: The support frame is arranged between the electromagnetic plate and the sample placement hole plate, and is used to support the sample placement hole plate. The support frame includes a plurality of support columns, and the plurality of support columns are support columns with adjustable support heights to adjust the magnetic strength of the electromagnetic plate.

5. The device according to claim 4, characterized in that The support heights of the multiple support columns are adjusted by rotation.

6. The device according to claim 3, characterized in that The sample mixing module comprises: The temperature integration submodule is arranged between the vortex instrument and the electromagnetic plate, and is used to provide corresponding reaction temperatures according to different experimental requirements.

7. The device according to claim 3, characterized in that The electromagnetic plate and the sample placement hole plate are provided with the same number of snaps at corresponding positions.

8. The device according to claim 3, characterized in that The electromagnetic plate and the vortex instrument are provided with the same number of buckles with corresponding positions.

9. The device according to claim 1, characterized in that The regeneration module comprises: A plurality of magnetic columns, used for absorbing the cleaned magnetic nanoparticles, wherein one end of the plurality of magnetic columns is fixed to the device frame; a disinfection submodule, fixed to the device frame, for disinfecting the magnetic nanoparticles; The drying submodule is at a height corresponding to the other ends of the plurality of magnetic columns and is used to dry the magnetic nanoparticles.

10. The device according to claim 9, characterized in that The plurality of magnetic poles are arranged in a matrix distribution; wherein the number of magnetic poles in each row is the same, and the positions of the magnetic poles in each column are aligned with each other.

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