Switching cover assembly for cell culture bottle and cell culture device

Abstract

The utility model provides a transfer cover assembly for a cell culture bottle and a cell culture device, the transfer cover assembly for the cell culture bottle is used for transferring a bottle opening and a bottle cap of the cell culture bottle, and comprises a transfer bottle cap, a transfer gland and a transfer head; two pipeline joints are arranged on the adapter gland, in an assembly state, the adapter is connected with the lower end of the adapter gland and the upper end of the adapter bottle cap, and the bottle cap channel is communicated with the gland channel; the transfer bottle cap is in threaded connection with a bottle opening of the cell culture bottle, and the cell culture bottle cap is in threaded connection with the upper end of the transfer gland; and an external port and an internal port of each pipeline joint are respectively connected with a standard pipeline. According to the transfer cap assembly for the cell culture bottle and the cell culture device, the process that an operator repeatedly opens and tightens the cell culture bottle cap during cell culture can be avoided, and liquid such as culture liquid enters and exits the culture bottle through a standard pipeline by special equipment, so that non-contact cell culture operation is realized; and the accuracy of experimental data is improved to a certain extent.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a transfer cover assembly for a cell culture bottle and a cell culture device. Background Art

[0002] Cell culture flasks are frequently used in cell culture, and aseptic operation is essential. With existing flask structures, operators must repeatedly unscrew and tighten the caps during the cell culture process. This is not only difficult and requires high technical skills, but the repeated unscrewing and tightening of the caps can also negatively impact experimental data, affecting its accuracy. Utility Model Content

[0003] The purpose of the utility model is to provide a transfer cover assembly for a cell culture flask and a cell culture device, so as to alleviate the above-mentioned technical problems existing in the prior art.

[0004] To achieve the above objectives, the present invention adopts the following technical solutions:

[0005] In a first aspect, an embodiment of the present invention provides an adapter cap assembly for a cell culture bottle, comprising an adapter bottle cap, an adapter pressure cap, and an adapter joint; an axially extending bottle cap channel is provided inside the adapter bottle cap, and an adapter internal thread is provided on the inner wall surface of the lower end of the bottle cap channel; an axially extending pressure cap channel is provided inside the adapter pressure cap, and an adapter external thread is provided on the outer wall surface of the upper end of the adapter pressure cap; two pipeline connectors are provided on the adapter pressure cap, and each of the pipeline connectors has one end extending to the outside of the adapter pressure cap and the other end extending to the inside of the pressure cap channel. In the assembled state: the adapter joint connects the lower end of the adapter pressure cap and the upper end of the adapter bottle cap, and the bottle cap channel is connected to the pressure cap channel.

[0006] In the technical solution of this embodiment, optionally, a flange protruding radially outward along the adapter cover is provided on the outer peripheral surface of the lower end of the adapter cover, and the pipeline joint axially passes through the flange and the two ends protrude from the flange upper surface and the flange lower surface of the flange respectively; in the assembled state: the adapter connects the flange and the upper end of the adapter bottle cap.

[0007] Further optionally, in the assembled state: the upper end surface of the adapter bottle cap abuts against the lower surface of the flange; the adapter joint is sleeved on the outside of the adapter pressure cap and the adapter bottle cap to fix the adapter pressure cap and the adapter bottle cap together.

[0008] Further optionally, the upper end of the adapter is provided with an annular boss extending radially inward along the adapter, and the inner circumferential surface of the lower end of the adapter is provided with an adapter internal thread; the outer circumferential surface of the upper end of the adapter bottle cap is provided with an adapter bottle cap external thread; in the assembled state: the lower surface of the annular boss abuts against the upper surface of the flange, and the adapter internal thread is connected to the adapter bottle cap external thread.

[0009] Optionally, a downwardly protruding limiting guide boss is further provided on the lower surface of the flange, and in the assembled state: the inner circumference of the upper end of the adapter bottle cap is clearance-matched with the outer circumference of the limiting guide boss.

[0010] Further optionally, the pipeline joint passes through the limiting guide boss.

[0011] In addition, in the technical solution of this embodiment, optionally, a barb is provided on the outer peripheral surface of the pipeline joint.

[0012] Optionally, a sealing step is further provided inside the bottle cap channel, and the sealing step includes a main body extending inward from the inner circumference of the bottle cap channel and a guide portion extending downward from the free end of the main body, and a draft angle is provided on one side surface of the lower end of the guide portion facing the inner circumference of the bottle cap channel.

[0013] In a second aspect, an embodiment of the present invention further provides a cell culture device, which includes a cell culture bottle, a cell culture bottle cap, four standard pipelines and an adapter cap assembly for a cell culture bottle according to any one of the aforementioned embodiments; in the assembled state: the adapter internal thread of the adapter bottle cap is threadedly connected to the bottle mouth of the cell culture bottle, and the cell culture bottle cap is threadedly connected to the adapter external thread of the adapter pressure cap; the external port and the internal port of each of the pipeline connectors are respectively connected to one of the standard pipelines, and of the two standard pipelines located inside the cell culture bottle, the pipeline end of one of the standard pipelines extends to the bottom of the cell culture bottle, and the pipeline end of the other standard pipeline is suspended in the air near the bottle mouth of the cell culture bottle.

[0014] In the cell culture device provided in this embodiment, optionally, a sealing step is further provided inside the bottle cap channel, wherein the sealing step includes a main body extending inward from the inner circumference of the bottle cap channel and a guide portion extending downward from the free end of the main body, and a draft angle is provided on a side surface of the lower end of the guide portion facing the inner circumference of the bottle cap channel; in the assembled state, the guide portion is inserted into and interference fits with the bottle mouth of the cell culture bottle.

[0015] Beneficial effects:

[0016] The cell culture flask adapter cap assembly and cell culture device provided in the present invention eliminate the need for operators to repeatedly open and tighten the caps during cell culture, preventing the entry of microorganisms, fungi, and other exogenous contaminants. Instead, specialized equipment allows for the transfer of culture fluid and other liquids into and out of the flask via standard pipelines, enabling contactless cell culture operations and improving the accuracy of experimental data to a certain extent. The invention also meets the requirements of specialized experimental conditions and is adaptable to flasks of varying sizes.

[0017] The specific usage and other beneficial effects of the embodiments of the present invention can be obtained by referring to the specific implementation methods section of this application specification. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 A schematic diagram of the overall structure of the adapter cap assembly for a cell culture flask provided in an embodiment of the present utility model;

[0020] Figure 2 for Figure 1 A schematic cross-sectional view of a transfer cap assembly for a cell culture flask is shown;

[0021] Figure 3 This is a schematic diagram of the overall structure of the cell culture device provided in an embodiment of the present utility model.

[0022] Icons: 1-adapter; 2-adapter bottle cap; 3-adapter pressure cap; 4-cell culture flask; 5-cell culture flask cap; 61-first standard pipeline; 62-second standard pipeline; 63-pipeline end; 7-pipeline connector; 8-adapter external thread; 9-adapter pressure cap upper end face; 10-flange lower surface; 101-limiting guide boss; 11-adapter internal thread; 12-flange upper surface; 13-adapter internal thread; 14-sealing step; 15-adapter bottle cap external thread; 16-adapter bottle cap upper end face. DETAILED DESCRIPTION

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are also within the scope of protection of the present invention.

[0025] It should be noted that like reference numerals and letters denote similar items in the drawings, and therefore, once an item is defined in one drawing, it does not require further definition or explanation in subsequent drawings.

[0026] In the description of this utility model, it should be noted that the terms "upper," "lower," "vertical," "horizontal," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is typically placed when in use. These terms are intended solely to facilitate the description of this utility model and simplify the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are used solely to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0027] Furthermore, the terms "horizontal" and "vertical" do not imply that a component must be absolutely horizontal or overhanging, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," not that the structure must be completely horizontal, but rather that it can be slightly tilted.

[0028] It should also be noted that, in the description of this utility model, unless otherwise expressly specified or limited, the terms "disposed," "installed," and "connected" should be understood broadly. For example, they may refer to fixed connections, detachable connections, or integral connections; they may refer to direct connections, indirect connections through an intermediate medium, or internal connections between two components. Those skilled in the art will understand the specific meanings of these terms in this utility model based on the specific circumstances.

[0029] The following embodiments of the present invention are described in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments may be combined with each other.

[0030] The first aspect of this embodiment provides a cell culture flask adapter cap assembly, referring to Figure 1 and Figure 2 The adapter cap assembly for a cell culture flask includes an adapter cap 2, an adapter gland 3, and an adapter joint 1. Specifically, an axially extending bottle cap channel is provided inside the adapter cap 2, and an adapter internal thread 13 is provided on the inner wall surface of the lower end of the bottle cap channel. An axially extending gland channel is provided inside the adapter gland 3, and an adapter external thread 8 is provided on the outer wall surface of the upper end of the adapter gland 3. Two pipeline connectors 7 are provided on the adapter gland 3, and each pipeline connector 7 has one end extending to the outside of the adapter gland 3 and the other end extending to the inside of the gland channel. In the assembled state: the adapter joint 1 connects the lower end of the adapter gland 3 and the upper end of the adapter cap 2, and the bottle cap channel is connected to the gland channel.

[0031] The second aspect of this embodiment provides a cell culture device, referring to Figure 3 , combined with Figure 1 and Figure 2 The cell culture device includes a cell culture bottle 4, a cell culture bottle cap 5, four standard pipelines (two first standard pipelines 61 and two second standard pipelines 62), and the aforementioned cell culture bottle adapter cap assembly; the top of the cell culture bottle cap 5 is provided with a breathable filter membrane.

[0032] In the assembled state: the adapter internal thread 13 of the adapter bottle cap 2 is threadedly connected to the bottle mouth of the cell culture bottle 4, and the cell culture bottle cap 5 is threadedly connected to the adapter external thread 8 on the upper end of the adapter gland 3; the external port and the internal port of each pipeline connector 7 are respectively connected to a standard pipeline, and one of the two standard pipelines located inside the cell culture bottle 4, the pipeline end 63 of one standard pipeline extends to the bottom of the cell culture bottle 4, and the pipeline end 63 of the other standard pipeline is suspended near the bottle mouth of the cell culture bottle 4. Specifically, Figure 3 As shown, the four standard pipelines include two first standard pipelines 61 and two second standard pipelines 62; the two first standard pipelines 61 serve as liquid outlet pipelines, respectively connected to the external port and internal port of a pipeline connector 7, and the pipeline end 63 of the first standard pipeline 61 connected to the internal port of the pipeline connector 7 extends to the bottom of the cell culture bottle 4; the two second standard pipelines 62 serve as liquid inlet pipelines, respectively connected to the external port and internal port of another pipeline connector 7, and the pipeline end 63 of the second standard pipeline 62 connected to the internal port of the pipeline connector 7 is suspended near the bottle mouth of the cell culture bottle 4.

[0033] When in use, the above-mentioned standard pipelines can be connected to the corresponding pipeline joints first, and the connection parts of each standard pipeline port and the corresponding pipeline joints can be fixed and sealed with light-curing glue; then, the adapter bottle cap 2 is docked with the adapter pressure cap 3, and the adapter bottle cap 2 and the adapter pressure cap 3 are fixed with the adapter joint 1; thereafter, the adapter internal thread 13 of the adapter bottle cap 2 is threadedly connected to the bottle mouth of the cell culture bottle 4, and the cell culture bottle cap 5 is threadedly connected to the adapter external thread 8 at the upper end of the adapter pressure cap 3, so that the cell culture device can realize the inflow and outflow of culture fluid and experimental liquid in and out of the cell culture bottle 4 through the standard pipeline, wherein the second standard pipeline The inlet line, formed by line 62, is used to deliver cells and associated culture fluids into the cell culture flask 4 via dedicated equipment. The outlet line, formed by the first standard line 61, is used to transport the cultured cells in the cell culture flask 4 to a collection device via dedicated equipment. The line end 63 of the first standard line 61, connected to the internal port of the corresponding line connector 7, extends to the bottom of the cell culture flask 4. This allows the cell culture flask 4 to be tilted toward the first standard line 61 at a certain angle to fully absorb the cultured cells and avoid loss. A breathable filter membrane is installed at the top of the cell culture flask cap 5 to allow cells to breathe during culture. Specifically, after the aforementioned components are assembled into a cell culture device, the assembled cell culture device can be sterile packaged and irradiated for sterilization before being removed for use in specific experiments.

[0034] The cell culture flask adapter cap assembly and cell culture device provided in this embodiment eliminate the need for operators to repeatedly open and tighten the cell culture flask cap 5 during cell culture, preventing the entry of microorganisms, fungi, and other exogenous contaminants during this process. Instead, specialized equipment allows for the transfer of culture fluid and other liquids into and out of the flask through standard pipelines, thereby achieving contactless cell culture operations and improving the accuracy of experimental data to a certain extent. This utility model has low requirements for experimental personnel and can meet the needs of some special experimental conditions.

[0035] The cell culture flask adapter cap assembly provided in this embodiment also has some optional structures for setting. Figures 1 to 3 , a more detailed description of its structure:

[0036] like Figure 1 and Figure 2 As shown, a flange protruding radially outward along the adapter gland 3 is provided on the outer circumference of the lower end of the adapter gland 3. The pipeline connector 7 axially passes through the flange and its two ends protrude from the flange upper surface 12 and flange lower surface 10 of the flange respectively. In the assembled state, the adapter 1 connects the flange and the upper end of the adapter bottle cap 2. Specific connection methods include but are not limited to: Figure 2As shown, the upper end surface 16 of the adapter bottle cap abuts against the lower surface 10 of the flange; the adapter 1 is sleeved on the outside of the adapter pressure cap 3 and the adapter bottle cap 2 to fix the adapter pressure cap 3 and the adapter bottle cap 2 together. This structure can use the upper end surface 16 of the adapter bottle cap and the lower surface 10 of the flange for docking and guidance. Furthermore, the upper end of the adapter 1 is provided with an annular boss extending radially inward along the adapter 1, and the inner circumference of the lower end of the adapter 1 is provided with an adapter internal thread 11; the outer circumference of the upper end of the adapter bottle cap 2 is provided with an adapter bottle cap external thread 15; in the assembled state: the lower surface of the annular boss of the adapter 1 abuts against the upper surface 12 of the flange, the internal thread 11 of the adapter is connected to the external thread 15 of the adapter bottle cap, and the annular boss of the adapter 1 provides a downward extrusion force, which cooperates with the internal thread 11 of the adapter to connect with the external thread 15 of the adapter bottle cap to improve the fit between the upper end surface 16 of the adapter bottle cap and the lower surface 10 of the flange. Furthermore, the flange lower surface 10 is also provided with a downwardly protruding limiting guide boss 101. In the assembled state, the upper inner circumference of the adapter bottle cap 2 is clearance-matched with the outer circumference of the limiting guide boss 101. This structure is conducive to providing a guide limit for rapid assembly on the one hand, and on the other hand, it cooperates with the adapter 1 to provide an extrusion force, which is conducive to the adapter bottle cap 2 and the adapter gland 3 being fixed by the adapter 1. The upper end surface 16 of the adapter bottle cap is tightly fitted with the flange lower surface 10, thereby ensuring the circumferential sealing of the adapter cap assembly. The specific setting position of the pipeline connector 7 is preferably through the limiting guide boss 101.

[0037] In addition, optionally and preferably, a barb is provided on the outer surface of the pipeline connector 7, and the standard pipeline can be interference fit with the barb structure when it is sleeved on the outside of the pipeline connector 7, thereby strengthening the stability of the connection structure between the standard pipeline and the pipeline connector 7 and preventing the standard pipeline from detaching from the pipeline connector 7.

[0038] In addition, in this embodiment, optionally and preferably, a sealing step 14 is further provided inside the bottle cap channel of the adapter bottle cap 2. The sealing step 14 includes a main body extending inward from the inner circumference of the bottle cap channel and a guide portion extending downward from the free end of the main body. The lower end of the guide portion is provided with a draft angle on one side surface facing the inner circumference of the bottle cap channel. In the cell culture device, the adapter internal thread 13 of the adapter bottle cap 2 is threadedly connected to the bottle mouth of the cell culture bottle 4. The guide portion of the sealing step 14 inside the bottle cap channel is inserted and interference fit into the bottle mouth of the cell culture bottle 4 under the guidance of its draft angle. The structure of the sealing step 14 can, on the one hand, ensure the stability and sealing of the connection structure between the adapter bottle cap 2 and the cell culture bottle 4. On the other hand, the structure of the sealing step 14 can be used as a compensating connection structure so that the adapter bottle cap 2 can be adapted to cell culture bottles 4 of different specifications.

[0039] Similarly, a structure similar to the sealing step 14 may be provided inside the cell culture bottle cap 5 so that after the cell culture bottle cap 5 is assembled with the adapter gland 3 , the upper end face 9 of the adapter gland is interference fit with the step structure inside the adapter gland for sealing connection.

[0040] Finally, it should be noted that: the various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other; the above embodiments in this specification are only used to illustrate the technical solution of the utility model, rather than to limit it; although the utility model is described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that: it is still possible to modify the technical solutions recorded in the aforementioned embodiments, or to replace some or all of the technical features therein with equivalents; and these modifications or replacements do not cause the essence of the corresponding technical solution to deviate from the scope of the technical solution of the various embodiments of the utility model.

Claims

1. A transfer cap assembly for a cell culture flask, characterized in that: It comprises a transfer bottle cap (2), a transfer pressure cap (3) and a transfer joint (1); The adapter bottle cap (2) is provided with an axially penetrating bottle cap channel inside, and the inner wall surface of the lower end of the bottle cap channel is provided with an adapter internal thread (13); An axially extending gland channel is provided inside the transition gland (3), and a transition external thread (8) is provided on the outer wall surface of the upper end of the transition gland (3); two pipeline connectors (7) are provided on the transition gland (3), and each pipeline connector (7) has one end extending to the outside of the transition gland (3) and the other end extending to the inside of the gland channel; In the assembled state: the adapter (1) connects the lower end of the adapter pressure cap (3) and the upper end of the adapter bottle cap (2), and the bottle cap channel is communicated with the pressure cap channel.

2. The adapter cap assembly for a cell culture flask according to claim 1, wherein: A flange protruding radially outward along the adapter gland (3) is provided on the outer peripheral surface of the lower end of the adapter gland (3); the pipeline connector (7) axially penetrates the flange and has two ends protruding from the flange upper surface (12) and the flange lower surface (10) of the flange respectively; In the assembled state: the adapter (1) connects the flange and the upper end of the adapter bottle cap (2).

3. The adapter cap assembly for a cell culture flask according to claim 2, wherein: In the assembled state: the upper end surface of the adapter bottle cap abuts against the lower surface (10) of the flange; the adapter (1) is sleeved on the outside of the adapter pressure cap (3) and the adapter bottle cap (2) to fix the adapter pressure cap (3) and the adapter bottle cap (2) together.

4. The adapter cap assembly for a cell culture flask according to claim 3, wherein: The upper end of the adapter (1) is provided with an annular boss extending radially inwardly along the adapter (1), and the inner circumferential surface of the lower end of the adapter (1) is provided with an adapter internal thread (11); the outer circumferential surface of the upper end of the adapter bottle cap (2) is provided with an adapter bottle cap external thread (15); in the assembled state: the lower surface of the annular boss abuts against the upper surface of the flange (12), and the adapter internal thread (11) is connected to the adapter bottle cap external thread (15).

5. The adapter cap assembly for a cell culture flask according to claim 3 or 4, characterized in that: The flange lower surface (10) is further provided with a downwardly protruding limiting guide boss (101). In the assembled state, the upper inner circumference of the adapter bottle cap (2) is clearance-matched with the outer circumference of the limiting guide boss (101).

6. The adapter cap assembly for a cell culture flask according to claim 5, wherein: The pipeline joint (7) passes through the limiting guide boss (101).

7. The adapter cap assembly for a cell culture flask according to claim 1, wherein: The outer peripheral surface of the pipeline joint (7) is provided with a barb.

8. The adapter cap assembly for a cell culture flask according to claim 1, wherein: A sealing step (14) is further provided inside the bottle cap channel. The sealing step (14) comprises a main body extending inward from the inner circumference of the bottle cap channel and a guide portion extending downward from the free end of the main body. A draft angle is provided on one side of the lower end of the guide portion facing the inner circumference of the bottle cap channel.

9. A cell culture device, characterized in that: Comprising a cell culture bottle (4), a cell culture bottle cap (5), four standard pipelines and a transfer cap assembly for a cell culture bottle according to any one of claims 1 to 7; In the assembled state: the transfer internal thread (13) of the transfer bottle cap (2) is threadedly connected to the bottle mouth of the cell culture bottle (4), and the cell culture bottle cap (5) is threadedly connected to the transfer external thread (8) of the transfer gland (3); The external port and the internal port of each pipeline connector (7) are respectively connected to one of the standard pipelines, and one of the two standard pipelines located inside the cell culture bottle (4) has a pipeline end (63) extending to the bottom of the cell culture bottle (4), and the pipeline end (63) of the other standard pipeline is suspended near the bottle mouth of the cell culture bottle (4).

10. The cell culture device according to claim 9, characterized in that: A sealing step (14) is further provided inside the bottle cap channel, the sealing step (14) comprising a main body extending inwardly from the inner circumference of the bottle cap channel and a guide portion extending downwardly from the free end of the main body, a draft angle being provided on one side of the lower end of the guide portion facing the inner circumference of the bottle cap channel; In the assembled state, the guide portion is inserted into and interference-fitted into the bottle mouth of the cell culture bottle (4).

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