Plate scribing equipment and bioassay system

Abstract

The utility model relates to the technical field of bioassay, and discloses plate scribing equipment and a bioassay system.The plate scribing equipment is characterized by comprising an inoculating loop, a first strain storage area, a plate supporting device, a movable scribing device and a first lifting device; and the inoculating loop is suitable for inoculating strains. The first strain storage area is suitable for placing vessels in which strains are stored; the plate supporting device is suitable for placing a plate. The inoculating loop is connected with the movable lineation device, and the movable lineation device is suitable for driving the inoculating loop to move horizontally so as to drive the inoculating loop to move between the position above the plate supporting device and the position above the first strain storage area and complete the lineation action. The device can replace most manual operation to carry out scribing operation, and does not need to worry that the working efficiency is reduced when the number of measured items is large and tasks are heavy. Compared with complete manual marking operation, the marking operation is faster, more timely and more accurate.

Description

Technical Field

[0001] The present invention relates to the technical field of bioassays, and in particular to a plate streaking device and a bioassay system. Background Art

[0002] Plate streaking, especially in microbiology experiments, is a commonly used inoculation and separation technique, usually used to dilute and separate microbial samples on the surface of solid culture medium to obtain pure cultured single colonies. It includes processes such as sterilization of the inoculation loop, bacteria collection and streaking. Specifically, it is necessary to prepare a sterile solid culture medium plate and ensure that it has solidified before inoculation. Before inoculation, the inoculation loop is sterilized to kill possible microorganisms and prevent contamination. After the inoculation loop is cooled, the inoculation operation is performed. Use the inoculation loop to pick a loop of bacterial liquid from the bacterial liquid to be inoculated. Then move the bacterial liquid on the inoculation loop to an area on the edge of the plate and streak it. After streaking the first area, you can continue to streak the second, third, and other areas as needed.

[0003] At present, the work of streaking the plate is completely dependent on manual operation. When there are many measurement items and heavy tasks, the efficiency of streaking the plate is easily reduced, resulting in the inability of manual operation to complete the streaking work quickly, timely and accurately. Utility Model Content

[0004] In view of this, the present invention provides a plate streaking device and a bioassay system to solve the problem that manual work cannot complete the plate streaking work quickly, timely and accurately.

[0005] In a first aspect, the present invention provides a plate streaking device, characterized in that it comprises:

[0006] Inoculating loop, suitable for picking up bacterial strains;

[0007] The first bacterial strain storage area is suitable for placing containers storing the bacterial strains;

[0008] A petri dish supporting device, suitable for placing the petri dish;

[0009] a mobile marking device, the inoculating loop being connected to the mobile marking device, the mobile marking device being adapted to drive the inoculating loop to move horizontally, so as to drive the inoculating loop to move between above the plate support device and above the first strain storage area and to complete a marking action;

[0010] A first lifting device is provided, wherein the movable marking device is connected to the first lifting device, and the first lifting device is adapted to drive the movable marking device to move up and down, so as to drive the inoculation loop to move up and down.

[0011] Optionally, it also includes:

[0012] The disinfection device is suitable for the inoculation loop to be inserted from above for disinfection, and the movable marking device is suitable for driving the inoculation loop to move between above the first strain storage area and above the disinfection device.

[0013] Optionally, it also includes:

[0014] The second strain storage area is suitable for placing a vessel storing the strains, and the movable marking device is suitable for driving the inoculation loop to move between above the second strain storage area and above the plate supporting device.

[0015] Optionally, it also includes:

[0016] A suction cup, suitable for adsorbing the lid of the petri dish;

[0017] The suction cup is connected to the moving device, and the moving device is suitable for driving the suction cup to move so as to move the suction cup above the plate.

[0018] A second lifting device is provided, wherein the moving device is connected to the second lifting device, and the second lifting device is suitable for driving the moving device to move up and down.

[0019] Optionally, the mobile device includes:

[0020] a first rocker arm driving device connected to the second lifting device;

[0021] The first rocker arm is connected to the first rocker arm driving device, the suction cup assembly is connected to the first rocker arm, and the first rocker arm driving device is suitable for driving the first rocker arm to rotate to drive the suction cup to move and be located above or leave the plate.

[0022] Optionally, the dish supporting device comprises:

[0023] a chuck, suitable for placing the petri dish;

[0024] A chuck driving device is provided, wherein the chuck is connected to the chuck driving device, and the chuck driving device is suitable for driving the chuck to tighten so as to clamp the plate.

[0025] Optionally, the mobile marking device includes:

[0026] a second rocker arm driving device connected to the first lifting device, wherein the first lifting device is adapted to drive the second rocker arm driving device to move upward and downward;

[0027] The second rocker arm is connected to the second rocker arm driving device, and the inoculation loop is connected to the second rocker arm. The second rocker arm driving device is suitable for driving the second rocker arm to rotate so that the inoculation loop moves between above the plate support device and above the first strain storage area.

[0028] Optionally, the mobile marking device further includes:

[0029] a third rocker arm driving device connected to the second rocker arm;

[0030] The third rocker arm is connected to the third rocker arm driving device, and the inoculation ring is connected to the third rocker arm. The third rocker arm driving device is suitable for driving the third rocker arm to rotate, so as to cooperate with the second rocker arm driving device to drive the second rocker arm to rotate and drive the inoculation ring to move linearly to complete the marking action.

[0031] Optionally, the disinfection device is configured as an infrared sterilizer.

[0032] In a second aspect, the present invention further provides a bioassay system comprising any of the above-mentioned plate streaking devices.

[0033] In this embodiment, during the marking operation, the first lifting device drives the mobile marking device to rise, thereby raising the inoculating loop. The mobile marking device then drives the inoculating loop to above the first bacterial strain storage area. The first lifting device then drives the mobile marking device down, driving the inoculating loop down as well. The inoculating loop is then inserted into the vessel containing the bacterial strains to extract the bacteria. After the extraction is complete, the first lifting device drives the mobile marking device down, driving the inoculating loop up as well. The mobile marking device then drives the inoculating loop along a preset path to above the plate support device. The first lifting device then drives the mobile marking device down, driving the inoculating loop down as well. The inoculating loop is then inserted into the plate, and the marking is completed using the mobile marking device to drive the inoculating loop. This method replaces most manual operations for marking, eliminating the need to worry about reduced work efficiency when there are many measurement items or a heavy workload. Compared to performing marking operations entirely manually, marking is faster, more timely, and more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in related technologies, the following briefly introduces the drawings required for use in the specific embodiments or related technical descriptions. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0035] Figure 1 This is an isometric view of a plate streaking device according to an embodiment of the present invention;

[0036] Figure 2 A top view of a plate scribing device according to an embodiment of the present invention;

[0037] Figure 3This is a front view of a plate scribing device according to an embodiment of the present invention;

[0038] Figure 4 The figure is a side view of a plate streaking device according to an embodiment of the present invention.

[0039] Description of reference numerals:

[0040] 1. Inoculation loop; 2. First culture storage area; 3. Petri dish support device; 31. Chuck; 32. Chuck drive device; 4. Moving marking device; 41. Second rocker arm drive device; 42. Second rocker arm; 43. Third rocker arm drive device; 44. Third rocker arm; 5. First lifting device; 6. Disinfection device; 7. Second culture storage area; 8. Suction cup; 9. Moving device; 91. First rocker arm drive device; 92. First rocker arm; 10. Base; 11. Petri dish; 12. Second lifting device. DETAILED DESCRIPTION

[0041] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative efforts shall fall within the scope of protection of the present invention.

[0042] The following combination Figures 1 to 4 , describing embodiments of the present invention.

[0043] According to an embodiment of the present invention, in one aspect, a plate streaking device is provided, comprising: an inoculating loop 1, a first bacterial seed storage area 2, a plate support device 3, a movable streaking device 4, and a first lifting device 5. The inoculating loop 1 is a commonly used inoculating loop 1 for receiving bacterial seeds. The first bacterial seed storage area 2 may be provided with a first placement slot, into which a vessel containing bacterial seeds is placed with its opening facing upward. A prepared plate 11 is placed on the plate support device 3. The first bacterial seed storage area 2, the plate support device 3, and the first lifting device 5 are all disposed on a base 10. The movable streaking device 4 is connected to the first lifting device 5 and can be used to drive the movable streaking device 4 upward and downward. The inoculating loop 1 is connected to the movable streaking device 4 and can be used to move the inoculating loop 1 along a preset path. The first bacterial seed storage area 2 and the plate support device 3 are both located below the preset path, so that the movable streaking device 4 can drive the inoculating loop 1 to move between above the plate support device 3 and above the first bacterial seed storage area 2.

[0044] Before streaking, place the petri dish 11 on the petri dish support 3, and place the vessel containing the bacterial strains in the first bacterial strain storage area 2. During streaking, disinfect the inoculating loop 1, and the first lifting device 5 drives the mobile streaking device 4 to rise, thereby raising the inoculating loop 1. The mobile streaking device 4 then drives the inoculating loop 1 along a preset path to above the first bacterial strain storage area 2. The first lifting device 5 then drives the mobile streaking device 4 down, lowering the inoculating loop 1. The inoculating loop 1 is then inserted into the vessel containing the bacterial strains to extract the bacteria. After extraction is complete, the first lifting device 5 drives the mobile streaking device 4 down, raising the inoculating loop 1. The mobile marking device 4 then drives the inoculating loop 1 along a preset path to above the plate support device 3. The first lifting device 5 then drives the mobile marking device 4 downward, thereby lowering the inoculating loop 1. The inoculating loop 1 is now inserted into the plate 11. The mobile marking device 4 then drives the inoculating loop 1 to move, completing the marking operation. After marking, the first lifting device 5 drives the mobile marking device 4 upward, thereby raising the inoculating loop 1, thus completing the marking operation. This method can replace most manual operations for marking, eliminating the need to worry about reduced work efficiency when there are many measurement items and heavy workloads. Compared to completely manual marking, it is faster, more timely, and more accurate.

[0045] If multiple markings are required, the inoculation loop 1 is disinfected after one marking operation, and then the mobile marking device 4 is driven down by the first lifting device 5 to drive the inoculation loop 1 down, and then the mobile marking device 4 drives the inoculation loop 1 to move and mark again.

[0046] The mobile marking device 4 and the first lifting device 5 are both connected to the control module for communication. The control module can be a programmable controller with a preset running program in the programmable controller, so that the movement of the mobile marking device 4 and the first lifting device 5 is controlled by the programmable controller and runs automatically.

[0047] In an optional practical method, the plate streaking equipment also includes a disinfection device 6, which can be an infrared sterilizer. After the inoculation loop 1 is inserted from the top of the infrared sterilizer, the infrared sterilizer can disinfect the inoculation loop 1. The disinfection device 6 is arranged on the base 10 and is located below the preset path for the movement of the inoculation loop 1. When the inoculation loop 1 is first disinfected during the streaking operation, the mobile streaking device 4 drives the inoculation loop 1 to move above the disinfection device 6, and the first lifting device 5 drives the mobile streaking device 4 to descend, thereby driving the inoculation loop 1 to descend. At this time, the inoculation loop 1 is inserted into the infrared sterilizer, and the infrared sterilizer is started to sterilize the inoculation loop 1. After the sterilization is completed, the first lifting device 5 drives the mobile streaking device 4 to rise, thereby driving the inoculation loop 1 to rise. Thereafter, the mobile streaking device 4 drives the inoculation loop 1 to move along the preset path to the top of the first bacterial strain storage area 2 for subsequent bacteria removal operations.

[0048] The disinfection device 6 is in communication with a control module, which may be a programmable controller having a pre-set operating program. When the first lifting device 5 drives the movable marking device 4 to descend, thereby lowering the inoculating loop 1, the control module automatically controls the disinfection device 6 to start. After disinfection is complete, the control module automatically controls the disinfection device 6 to shut down.

[0049] In some embodiments, the plate streaking device further includes a second bacterial strain storage area 7. This second bacterial strain storage area 7 can be provided with a second placement slot. Vessels storing other bacterial strains are placed in this second placement slot in the second bacterial strain storage area 7 with their openings facing upward. This means that the bacterial strains stored in the vessels in the second bacterial strain storage area 7 differ from those stored in the vessels in the first bacterial strain storage area 2. Depending on the needs, either the second bacterial strain storage area 7 or the first bacterial strain storage area 2 can be selected to store vessels storing other bacterial strains.

[0050] The second culture storage area 7 is located below a preset path, so that the mobile marking device 4 can drive the inoculating loop 1 to move between above the plate support device 3 and above the first culture storage area 2. When removing bacteria, the mobile marking device 4 drives the inoculating loop 1 along the preset path to above the first culture storage area 2 or the second culture storage area 7. The first lifting device 5 drives the mobile marking device 4 to descend, thereby driving the inoculating loop 1 downward. At this time, the inoculating loop 1 is inserted into the vessel storing the bacteria to remove bacteria. After the bacteria are removed, the first lifting device 5 drives the mobile marking device 4 to descend, driving the inoculating loop 1 to rise.

[0051] In an optional embodiment, the plate streaking apparatus further includes a suction cup 8, a moving device 9, and a second lifting device 12. The interior of the suction cup 8 is connected to a vacuum pump via a pipeline, which allows the vacuum pump to draw air from the suction cup 8. The suction cup 8 is connected to the moving device 9. The second lifting device 12 is mounted on a base 10 and positioned to one side of the plate support 3. The second lifting device 12 can be a vertically mounted electric cylinder. The moving device 9 is connected to the second lifting device 12, which drives the moving device 9 upward and downward. The moving device 9 drives the suction cup 8 to move above the plate 11.

[0052] With this arrangement, before streaking, the dish cover is placed on the dish 11 to prevent contamination of the culture medium within the dish 11. To streak, the second lifting device 12 is used to drive the movable device 9 upward, thereby raising the suction cup 8. The movable device 9 drives the suction cup 8 to move above the dish 11. The second lifting device 12 is then used to drive the movable device 9 downward, driving the suction cup 8 downward to contact the dish cover. At this point, the vacuum pump is activated to suction air into the suction cup 8, causing the suction cup 8 to lift the dish cover. The second lifting device 12 is then used to drive the movable device 9 upward, raising the suction cup 8 and the dish cover, thereby opening the dish cover. The movable device 9 is used to drive the suction cup 8 away from above the dish 11, allowing the inoculating loop 1 to descend and penetrate the dish 11 for streaking.

[0053] The vacuum pump, the moving device 9 and the second lifting device 12 are respectively connected to the control module for communication. The control module can be a programmable controller. A running program is preset in the programmable controller to automatically control the moving device 9 to drive the suction cup 8 to move above the plate 11, and then control the vacuum pump to start and the suction cup 8 to suck up the plate cover.

[0054] Furthermore, the moving device 9 includes a first rocker arm drive device 91 and a first rocker arm 92. The first rocker arm drive device 91 can be a motor. The first rocker arm drive device 91 is connected to the second lifting device 12, and the first end of the first rocker arm 92 is connected to the first rocker arm drive device 91, so that the first rocker arm drive device 91 can drive the second end of the first rocker arm 92 to rotate about the first end. The suction cup 8 is connected to the second end of the first rocker arm 92. When the first rocker arm drive device 91 can drive the first rocker arm 92 to rotate, the suction cup 8 rotates about the first end of the first rocker arm 92. The plate support device 3 is located below the movement path of the suction cup 8. Therefore, when the first rocker arm drive device 91 drives the suction cup 8 to rotate, the suction cup 8 can move above the plate 11 on the plate support device 3. The second lifting device 12 drives the moving device 9 to descend, causing the suction cup 8 to descend and contact the plate cover. After the suction cup 8 has lifted the lid of the dish 11, the second lifting device 12 drives the moving device 9 to rise, thereby raising the suction cup 8 and the lid, thereby opening the lid. The first rocker drive device 91 drives the suction cup 8 to rotate, and the suction cup 8 sucks the lid off the top of the dish 11.

[0055] In an alternative embodiment, the plate support device 3 includes a chuck 31 and a chuck drive 32. The chuck 31 is connected to the chuck drive 32, which can be used to tighten and expand the chuck 31. After the chuck drive 32 expands the chuck 31, the plate 11 is placed on the chuck 31. The chuck drive 32 then tightens the chuck 31, thereby clamping the plate 11 and securing it to the plate support device 3. This prevents the plate 11 from moving during marking, which could affect the marking effect.

[0056] The chuck 31 is a pneumatic chuck 31, and the chuck driving device 32 is a cylinder, which drives the pneumatic chuck 31 to tighten and open. It is worth noting that the pneumatic chuck 31 is a commonly used device in the field, and its principle will not be repeated.

[0057] In an optional embodiment, the first lifting device 5 may be a vertically arranged electric cylinder, and the movable marking device 4 is connected to the electric cylinder so that the electric cylinder can drive the movable marking device 4 to move up and down.

[0058] In some embodiments, the movable marking device 4 includes: a second rocker arm drive device 41 and a second rocker arm 42. The second rocker arm drive device 41 can be a motor, and the second rocker arm drive device 41 is connected to an electric cylinder so that the electric cylinder can drive the second rocker arm drive device 41 to move up and down. The first end of the second rocker arm 42 is connected to the second rocker arm drive device 41, so that the second rocker arm drive device 41 can drive the second end of the second rocker arm 42 to rotate around the first end, and the inoculation loop 1 is connected to the second end of the second rocker arm 42. Therefore, when the second rocker arm drive device 41 drives the second rocker arm 42 to rotate, the inoculation loop 1 is driven to move along a preset path, that is, the inoculation loop 1 is driven to move between above the plate support device 3 and above the first culture storage area 2.

[0059] In a further embodiment, it is characterized in that the mobile marking device 4 further includes: a third rocker arm driving device 43 and a third rocker arm 44. The third rocker arm driving device 43 can be a motor. The first end of the third rocker arm 44 is connected to the third rocker arm driving device 43, so that the third rocker arm driving device 43 can drive the second end of the third rocker arm 44 to rotate around the first end. The inoculation loop 1 is connected to the second end of the third rocker arm 44, so that when the third rocker arm driving device 43 drives the third rocker arm 44 to rotate, the inoculation loop 1 is driven to rotate around the first end of the third rocker arm 44. The third rocker arm driving device 43 is connected to the second end of the second rocker arm 42, so that when the second rocker arm driving device 41 drives the second rocker arm 42 to rotate, it can drive the inoculation loop 1 to move along a preset path.

[0060] Moreover, after the inoculation loop 1 is inserted into the plate 11, the third rocker arm drive device 43 drives the inoculation loop 1 to move along an arc, and at the same time, the second rocker arm drive device 41 drives the third rocker arm drive device 43 to move along an arc for motion compensation, so that the third rocker arm drive device 43 and the second rocker arm drive device 41 cooperate to drive the inoculation loop 1 to move in a straight line to perform the marking operation.

[0061] According to an embodiment of the present invention, on the other hand, a bioassay system is provided, comprising any of the above-mentioned plate streaking devices. The beneficial effects of the bioassay system are the same as those of the plate streaking device, and thus will not be described in detail.

[0062] Although the embodiments of the present invention have been described with reference to the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention. Such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A plate streaking device, characterized in that: include: An inoculating loop (1), suitable for picking up bacterial strains; A first bacterial strain storage area (2) is suitable for placing containers storing the bacterial strains; A plate supporting device (3) suitable for placing the plate (11); a mobile marking device (4), the inoculation loop (1) being connected to the mobile marking device (4), and the mobile marking device (4) being adapted to drive the inoculation loop (1) to move horizontally, so as to drive the inoculation loop (1) to move between above the plate support device (3) and above the first bacterial strain storage area (2) and to complete a marking action; A first lifting device (5), the movable marking device (4) is connected to the first lifting device (5), and the first lifting device (5) is suitable for driving the movable marking device (4) to move up and down, so as to drive the inoculation ring (1) to move up and down.

2. The plate streaking device according to claim 1, characterized in that: Also includes: The disinfection device (6) is suitable for the inoculation loop (1) to be inserted from above for disinfection, and the movable marking device (4) is suitable for driving the inoculation loop (1) to move between above the first strain storage area (2) and above the disinfection device (6).

3. The plate streaking device according to claim 1, characterized in that: Also includes: The second strain storage area (7) is suitable for placing a vessel storing the strain, and the movable marking device (4) is suitable for driving the inoculation loop (1) to move between above the second strain storage area (7) and above the plate support device (3).

4. The plate streaking device according to claim 1, characterized in that: Also includes: A suction cup (8) adapted to absorb the lid of the plate (11); a moving device (9), the suction cup (8) being connected to the moving device (9), and the moving device (9) being adapted to drive the suction cup (8) to move so as to move the suction cup (8) above the plate (11); A second lifting device (12), the moving device (9) is connected to the second lifting device (12), and the second lifting device (12) is suitable for driving the moving device (9) to rise and fall.

5. The plate streaking device according to claim 4, characterized in that: The mobile device (9) comprises: A first rocker arm driving device (91) connected to the second lifting device (12); The first rocker arm (92) is connected to the first rocker arm driving device (91), and the suction cup (8) assembly is connected to the first rocker arm (92). The first rocker arm driving device (91) is suitable for driving the first rocker arm (92) to rotate, so as to drive the suction cup (8) to move and be located above or leave the plate (11).

6. The plate streaking device according to claim 1, characterized in that: The plate supporting device (3) comprises: a chuck (31), suitable for placing the petri dish (11); A chuck driving device (32), the chuck (31) is connected to the chuck driving device (32), and the chuck driving device (32) is suitable for driving the chuck (31) to tighten so as to clamp the plate (11).

7. The plate streaking device according to claim 1, characterized in that: The mobile marking device (4) comprises: a second rocker arm driving device (41) connected to the first lifting device (5), wherein the first lifting device (5) is adapted to drive the second rocker arm driving device (41) to move up and down; The second rocker arm (42) is connected to the second rocker arm driving device (41), and the inoculation loop (1) is connected to the second rocker arm (42). The second rocker arm driving device (41) is suitable for driving the second rocker arm (42) to rotate so that the inoculation loop (1) moves between above the plate supporting device (3) and above the first strain storage area (2).

8. The plate streaking device according to claim 7, characterized in that: The mobile marking device (4) further comprises: a third rocker arm driving device (43), connected to the second rocker arm (42); The third rocker arm (44) is connected to the third rocker arm driving device (43), and the inoculation ring (1) is connected to the third rocker arm (44). The third rocker arm driving device (43) is suitable for driving the third rocker arm (44) to rotate, so as to cooperate with the second rocker arm driving device (41) to drive the second rocker arm (42) to rotate and drive the inoculation ring (1) to move linearly to complete the marking action.

9. The plate streaking device according to claim 2, characterized in that: The disinfection device (6) is configured as an infrared sterilizer.

10. A bioassay system, characterized in that: include: A device for marking a plate (11) according to any one of claims 1 to 9.

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