Total number of bacterial colonies detection assembly and bioassay system

Abstract

The utility model relates to the technical field of bioassay, and discloses a total bacterial colony number detection assembly and a bioassay system. Comprising a sample area for placing a sample container, a diluent area for placing a diluent container, a preparation container area for placing a preparation container, a carrier area for placing a carrier, an incubator, a conveying device and a bacterial colony counting device which are respectively arranged on a platform. A space moving device is arranged on the platform, is connected with the grabbing mechanism and the pipette, and is suitable for driving the grabbing mechanism and the pipette to move in a space above the platform. The grabbing mechanism is suitable for grabbing objects, the pipette is suitable for sucking and discharging liquid, and the conveying device is suitable for placing a carrier and driving the carrier to enter and exit from the incubator. By means of the total bacterial colony number detection assembly, manual work can be replaced for the partial detection process of the total bacterial colony number, the influence of limited detection personnel resources and multiple detection items is avoided, and compared with the mode that the total bacterial colony number is detected completely through manual work, the working efficiency is higher, and the accuracy is better.

Description

Technical Field

[0001] The present application relates to the field of bioassay technology, and in particular to a total bacterial count detection component and a bioassay system. Background Art

[0002] A colony is a group of daughter cells, visible to the naked eye and characterized by morphology and structure, formed by a single or a small number of microbial cells growing and multiplying to a certain extent on or within a suitable solid culture medium, centered around a mother cell. The total bacterial colony count refers to the total number of bacterial colonies grown per gram (per milliliter) of sample under specific conditions (e.g., oxygen demand, nutrient conditions, pH, culture temperature, and time).

[0003] For the detection of total colony count, the sample to be tested is generally made into several different 10-fold incremental dilutions, and then 1 mL is taken out from each dilution and placed in a sterile plate and mixed with nutrient agar medium. After incubation at a certain temperature for a certain period of time (generally 48 hours), the number of colonies formed in each plate is recorded, and the total number of bacterial colonies contained in each gram (or each ml) of the original sample is calculated based on the dilution multiple.

[0004] Currently, the work of detecting the total colony count is done entirely manually. Due to limited testing personnel resources and a large number of inspection items, the efficiency and accuracy of detecting the total colony count are low. Utility Model Content

[0005] In view of this, the present application provides a total colony count detection component and a bioassay system to solve or improve the problems of low work efficiency and poor accuracy in manual detection of total colony count.

[0006] In a first aspect, the present application provides a total colony count detection assembly, comprising a sample area suitable for placing a sample container, a diluent area suitable for placing a diluent container, a preparation container area suitable for placing a preparation container, a carrier area suitable for placing a carrier, an incubator, a conveying device, and a colony counting device suitable for detecting the total colony count, respectively arranged on a platform, wherein:

[0007] A space moving device is provided on the platform, the space moving device is connected to a grabbing mechanism and a pipette, and the space moving device is suitable for driving the grabbing mechanism and the pipette to move in the space above the platform;

[0008] The gripping mechanism is suitable for gripping the sample container, the diluent container, the preparation container or the carrier;

[0009] The pipette is suitable for sucking a sample from the sample container, sucking a diluent from the diluent container, and discharging the sucked liquid into the diluent container, sucking a liquid from the preparation container, and discharging the sucked liquid into the preparation container, or discharging the sucked liquid into the carrier;

[0010] The transport device is suitable for placing the carrier and driving the carrier into and out of the incubator.

[0011] Optionally, it also includes:

[0012] a first barcode scanning device, disposed on the platform and adapted to scan a first barcode on the sample container;

[0013] a second barcode scanning device, disposed on the platform and adapted to scan a second barcode on the preparation container;

[0014] The third barcode scanning device is arranged on the platform and is suitable for scanning the third barcode on the carrier.

[0015] Optionally, it also includes:

[0016] a first oscillating device, disposed on the platform and suitable for placing the diluent container, wherein the first oscillating device can shake the diluent container;

[0017] A second oscillating device is arranged on the platform and is suitable for placing the preparation container. The second oscillating device can shake the preparation container.

[0018] Optionally, it also includes:

[0019] An automatic capping machine is arranged on the platform and is suitable for placing sample containers and opening the caps of the sample containers.

[0020] Optionally, the carrier is a petri dish, the carrier area is provided with a petri dish rack for placing the petri dish, the platform is provided with a culture medium area suitable for placing a culture medium container, the grasping mechanism is also suitable for grasping the culture medium container, and the pipette is suitable for drawing culture medium from the culture medium container.

[0021] Optionally, it also includes:

[0022] A third oscillating device is provided on the platform and is suitable for placing the culture medium container. The third oscillating device can shake the culture medium container.

[0023] Optionally, it also includes:

[0024] The plate shaking mechanism is arranged on the platform and is suitable for placing the plate. The plate shaking mechanism can shake the plate to make it uniform.

[0025] Optionally, the carrier is a test piece, and the carrier area is provided with a sheet pressing device for placing the test piece.

[0026] Optionally, it also includes:

[0027] an acid-base test paper area, disposed on the platform and suitable for placing acid-base test paper;

[0028] The visual mechanism is arranged on the platform and is suitable for scanning and detecting the acid-base test paper.

[0029] In a second aspect, the present application also provides a bioassay system comprising any of the above-described total colony count detection components.

[0030] The present application provides a total bacterial count detection assembly comprising a sample area, a diluent area, a preparation container area, a carrier area, an incubator, a conveyor device, and a colony counting device, each disposed on a platform. A spatial movement device is disposed on the platform, connected to a gripping mechanism and a pipette. The spatial movement device is capable of driving the gripping mechanism and the pipette to move within the space above the platform.

[0031] When the total colony count of a sample needs to be tested, the sample container containing the sample is placed in the sample area. Use a pipette to draw 25 ml of sample from the sample container and discharge it into the first diluent container containing 225 ml of diluent, thereby making a 1:10 sample solution in the first diluent container. Use a pipette to draw 9 ml of physiological saline diluent from the second diluent container and discharge it into the first preparation container. Then use a pipette to draw 1 ml of the 1:10 sample solution from the first diluent container and discharge it into the first preparation container, thereby making a 1:100 sample solution in the first preparation container. Use a pipette to draw 9 ml of physiological saline diluent from the second diluent container and discharge it into the second preparation container. Then use a pipette to draw 1 ml of the 1:10 sample solution from the first preparation container and discharge it into the second preparation container, thereby making a 1:1000 sample solution in the second preparation container. Use a pipette to draw 1ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution respectively and discharge them into corresponding carriers. Use a grabbing mechanism to grab each carrier and place it on a conveying device. Use the conveying device to send each carrier into an incubator. After each carrier is cultured in the incubator, use the conveying device to send each carrier out of the incubator. Finally, use a grabbing mechanism to grab each carrier and place it on a colony counting device. Use the colony counting device to calculate the total number of colonies in each carrier, and then calculate the total number of colonies in the 1ml sample.

[0032] In this way, part of the total colony count detection process can be replaced by manual labor, and it is not affected by limited testing personnel resources and a large number of inspection items. Compared with completely manual testing of the total colony count, it has higher work efficiency and better accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the specific implementation methods of this application or the technical solutions in related technologies, the following is a brief introduction to the drawings required for use in the specific implementation methods or related technical descriptions. Obviously, the drawings described below are some implementation methods of this application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0034] Figure 1 A top view of a total bacterial count detection assembly according to an embodiment of the present application;

[0035] Figure 2 This is a first-perspective isometric view of a total bacterial count detection assembly according to an embodiment of the present application;

[0036] Figure 3 This is a second perspective isometric view of a total colony count detection component according to an embodiment of the present application.

[0037] Description of reference numerals:

[0038] 1. Platform; 2. Sample area; 3. Dilution area; 4. Culture medium area; 5. Preparation container area; 6. Incubator; 7. Conveying device; 8. Colony counting device; 9. First code scanning device; 10. Second code scanning device; 11. Third code scanning device; 12. First oscillation device; 13. Second oscillation device; 14. Third oscillation device; 15. Automatic capping machine; 16. Test tube decapping machine; 17. Tablet pressing device; 18. Petri dish rack; 19. Pipette; 20. Grabbing mechanism; 21. Spatial moving device; 22. Petri dish shaking mechanism; 23. Visual mechanism; 24. Waste area. DETAILED DESCRIPTION

[0039] To make the purpose, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative efforts shall fall within the scope of protection of this application.

[0040] The following combination Figures 1 to 3 , describing the embodiments of the present application.

[0041] According to an embodiment of the present application, on the one hand, Figures 1 to 3As shown, a total colony count detection assembly is provided, comprising a sample area 2, a diluent area 3, a preparation container area 5, a carrier area, an incubator 6, a conveying device 7 and a colony counting device 8. The colony counting device 8 may be a colony counter.

[0042] The sample area 2 is arranged on the platform 1 and is provided with a sample rack. The sample rack is provided with a plurality of sample placement slots. Sample containers containing samples are placed in the placement slots on the sample rack.

[0043] The diluent area 3 is provided on the platform 1 and is provided with a diluent rack, which is provided with a plurality of placement slots, and the diluent containers containing physiological saline diluent are placed in the placement slots on the diluent rack. The diluent containers can be triangular flasks.

[0044] The preparation container area 5 is arranged on the platform 1 and is provided with a container rack, which is provided with a plurality of placement slots, and the preparation containers are placed in the placement slots on the container rack. The preparation containers can be test tubes.

[0045] The carrier area is arranged on the platform 1 and is provided with a plurality of carriers, wherein the carriers can be flat dishes or test pieces.

[0046] The incubator 6 is provided on the platform 1 . After the carrier is placed in the incubator 6 , the incubator 6 can incubate the carrier at a certain temperature.

[0047] The conveying device 7 is arranged on the platform 1. After the carrier is placed on the conveying device 7, the conveying device 7 can drive the carrier into the incubator 6. After the incubation is completed, the conveying device 7 can drive the carrier out of the incubator 6.

[0048] The colony counting device 8 is arranged on the platform 1 . After the carrier is placed in the colony counting device 8 , the colony counting device 8 can detect and count the total number of colonies on the carrier.

[0049] like Figure 2 and Figure 3 As shown, a spatial moving device 21 is provided on the platform 1, to which a gripping mechanism 20 and a pipette 19 are connected. The spatial moving device 21 can drive the gripping mechanism 20 and the pipette 19 to move in the space above the platform 1. The spatial moving device 21 can be a truss manipulator, and the gripping mechanism 20 can be a gripper.

[0050] A truss robot is a fully automated industrial device based on a rectangular X, Y, and Z coordinate system, capable of adjusting workpiece positions and achieving trajectory motion. Its control core is implemented by an industrial controller (such as a PLC, motion control system, or single-chip microcomputer). The controller analyzes and processes various input signals (such as sensors and buttons), makes logical judgments, and then issues execution commands to various output components (such as relays, motor drivers, and indicator lights), completing the coordinated movement of the X, Y, and Z axes, thereby achieving a fully automated operation process.

[0051] The truss manipulator consists of a structural frame, X-axis, Y-axis, and Z-axis assemblies, a fixture, and a control cabinet. The structural frame is attached to platform 1. Under control of the control cabinet, the X-axis, Y-axis, and Z-axis assemblies drive the fixture along the X, Y, and Z axes, respectively. The gripping mechanism 20 and pipette 19 are each mounted on the fixture, enabling them to move within the space above platform 1.

[0052] In this way, under the action of the spatial moving device 21 , the grabbing mechanism 20 can grab any one of the sample container, the diluent container, the preparation container or the carrier and move it in the space above the platform 1 .

[0053] While the pipette 19 moves in the space above the platform 1, the pipette 19 can absorb samples from the sample container, absorb diluent from the diluent container, and discharge the absorbed liquid into the diluent container, absorb culture medium from the culture medium container, absorb liquid from the preparation container, and discharge the absorbed liquid into the preparation container, and discharge the absorbed liquid into the carrier.

[0054] The spatial moving device 21, the grasping mechanism 20 and the pipette gun 19 can all be connected to a control module to facilitate automatic control.

[0055] When the total colony count of the sample needs to be detected, the sample container containing the sample is placed in the sample area 2. After the lid of the sample container is opened, 25 ml of sample is aspirated from the sample container using a pipette 19 and then discharged into the first diluent container containing 225 ml of diluent, thereby preparing a 1:10 sample solution in the first diluent container.

[0056] 9 ml of saline diluent was drawn from the second diluent container using a pipette 19 and discharged into the first preparation container. 1 ml of the 1:10 sample solution was then drawn from the first diluent container using a pipette 19 and discharged into the first preparation container, thereby producing a 1:100 sample solution in the first preparation container.

[0057] 9 ml of saline diluent was drawn from the second diluent container using the pipette 19 and discharged into the second preparation container. 1 ml of the 1:10 sample solution was then drawn from the first preparation container using the pipette 19 and discharged into the second preparation container, thereby preparing a 1:1000 sample solution in the second preparation container.

[0058] Use the pipette 19 to respectively draw 1ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution and discharge them into corresponding carriers. Use the grabbing mechanism 20 to grab each carrier and place it on the conveying device 7. Use the conveying device 7 to respectively transport each carrier into the incubator 6. After each carrier is cultured in the incubator 6, use the conveying device 7 to respectively transport each carrier out of the incubator 6. Finally, use the grabbing mechanism 20 to grab each carrier and place it on the colony counting device 8. Use the colony counting device 8 to calculate the total number of colonies in each carrier, and then calculate the total number of colonies in the 1ml sample.

[0059] This setting replaces part of the manual detection process of the total colony count, is not affected by the limited resources of detection personnel and the large number of inspection items, and is more efficient and accurate than completely manual detection of the total colony count.

[0060] The conveyor 7 can be a conventional linear module with a tray mounted on its movable portion, enabling the linear module to drive the tray between a first position and a second position. In the first position, the tray is outside the incubator 6, while in the second position, it is inside the incubator 6. Thus, after placing a carrier on the tray in the first position, the linear module drives the tray to the second position, thereby transporting the carrier into the incubator 6. After incubation is complete, the linear module drives the tray back to the first position.

[0061] The conveying device 7 can also be a belt conveyor that can move back and forth. A tray is set on the conveyor belt of the belt conveyor. The conveyor belt drives the tray to move between a first position and a second position. When the tray is in the first position, it is outside the incubator 6. When the tray is in the second position, it is inside the incubator 6.

[0062] The conveying device 7 can also be a motor-driven screw structure, with a tray mounted on a screw nut mounted on the screw. The motor drives the screw to rotate, thereby driving the tray to move between a first position and a second position. When the tray is in the first position, it is located outside the incubator 6, and when the tray is in the second position, it is located inside the incubator 6.

[0063] At least two pipetting guns 19 can be provided to improve operation efficiency.

[0064] There can be multiple incubators 6 , each of which can be set to a different temperature to meet different needs. Correspondingly, a conveying device 7 is provided for each incubator 6 .

[0065] In one embodiment, if Figure 1 As shown, the total bacterial count detection component also includes a first barcode scanning device 9, a second barcode scanning device 10, and a third barcode scanning device 11. The first barcode scanning device 9, the second barcode scanning device 10, and the third barcode scanning device 11 are all set on the platform 1 and are connected to the LIMS management system in communication, so that the information scanned by the first barcode scanning device 9, the second barcode scanning device 10, and the third barcode scanning device 11 can be sent to the LIMS management system for storage.

[0066] The sample container is preset with a first barcode. After the grabbing mechanism 20 grabs a sample container, it moves to the first barcode scanning device 9. The first barcode scanning device 9 scans the first barcode on the sample container and sends the information to the LIMS management system, thereby recording the information of the grabbed sample container.

[0067] A second barcode is preset on the preparation container. After the grasping mechanism 20 grasps a preparation container, it moves to the second barcode scanning device 10. The second barcode scanning device 10 scans the second barcode on the preparation container and sends the information to the LIMS management system, thereby recording the information of the grasped preparation container and establishing a correspondence between the sample container and the preparation container.

[0068] A third barcode is provided on the carrier. After the grabbing mechanism 20 grabs a carrier, it moves to the third barcode scanning device 11. The third barcode scanning device 11 scans the third barcode on the carrier and sends the information to the LIMS management system, thereby recording the information of the grabbed carrier and establishing a correspondence between the sample container, preparation container and carrier.

[0069] In one embodiment, if Figures 1 to 3 As shown, the total bacterial count detection assembly further includes a first oscillating device 12 and a second oscillating device 13. The first oscillating device 12 and the second oscillating device 13 can be commonly used laboratory oscillators, and their structures and principles are not described in detail.

[0070] The first oscillating device 12 is provided on the platform 1 . After the diluent container is placed on the first oscillating device 12 , the first oscillating device 12 is started to oscillate the diluent container to evenly shake the liquid in the diluent container.

[0071] With this arrangement, after 25 ml of sample is discharged into the first diluent container containing 225 ml of diluent, the first diluent container is grabbed by the gripping mechanism 20 and brought to the first oscillating device 12. The first oscillating device 12 is used to shake the liquid in the first diluent container to prepare a more uniform 1:10 sample solution. After shaking, the first diluent container is grabbed by the gripping mechanism 20 and returned to the diluent area 3.

[0072] The second oscillating device 13 is provided on the platform 1 . After the preparation container is placed on the first oscillating device 12 , the second oscillating device 13 is started to oscillate the preparation container to evenly shake the liquid in the preparation container.

[0073] In this configuration, after 9 ml of saline diluent and 1 ml of a 1:10 sample solution are placed in the first preparation container, the first preparation container is grasped by the gripping mechanism 20 and brought to the second oscillating device 13. The second oscillating device 13 is used to shake the liquid in the first preparation container to produce a more uniform 1:100 sample solution. After the shaking is complete, the first preparation container is grasped by the gripping mechanism 20 and returned to the preparation container area 5.

[0074] After placing 9 ml of the saline diluent and 1 ml of the 1:100 sample solution into the second preparation container, the gripping mechanism 20 is used to grab the second preparation container and bring it to the second oscillating device 13. The second oscillating device 13 is used to shake the liquid in the second preparation container to prepare a more uniform 1:1000 sample solution. After shaking, the gripping mechanism 20 is used to grab the second preparation container and return it to the preparation container area 5.

[0075] In one embodiment, Figure 1 As shown, the total bacterial count detection assembly also includes an automatic capping machine 15. The automatic capping machine 15 is mounted on the platform 1. The automatic capping machine 15 can be a commonly used automatic capping device for preparing a line, and its structure and principle are not described in detail here. After the sample container is placed on the automatic capping machine 15, the automatic capping machine 15 opens the container lid, allowing the pipette 19 to draw the sample from the sample container. This eliminates the need for manual capping, further improving efficiency.

[0076] In one embodiment, Figure 1 As shown, the total bacterial count detection assembly also includes a test tube decapping machine 16. This machine is mounted on platform 1 and can be a conventional test tube decapping device. Its structure and operating principle are not described in detail here. After a test tube, serving as a preparation container, is placed on the decapping machine 16, it removes the cap, allowing a pipette 19 to draw a sample from the test tube and inject liquid into the test tube. This eliminates the need for manual decapping, further improving efficiency.

[0077] In one embodiment, Figures 1 to 3 As shown, the carrier is a petri dish, and a petri dish rack 18 is provided in the carrier area. The petri dish rack 18 is provided with a placement groove, and the petri dish can be placed in the placement groove on the petri dish rack 18.

[0078] A culture medium area 4 is provided on platform 1. A dry bath is provided in this area. A culture medium container containing culture medium is placed in the dry bath and stored at 48°C. The culture medium container can be a conical flask. A gripping mechanism 20 can grip the culture medium container, and a pipette 19 can aspirate the culture medium from the culture medium container.

[0079] Thus, first, 15 ml of culture medium is drawn from the culture medium container into a petri dish using the pipette 19, and then 1 ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution are placed into the petri dish using the pipette 19. Similarly, 1 ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution are placed into the corresponding petri dishes, respectively.

[0080] The grabbing mechanism 20 grabs each plate and places it on the conveying device 7. The conveying device 7 then transports each plate into the incubator 6. After each plate is incubated in the incubator 6, the conveying device 7 transports each plate out of the incubator 6. Finally, the grabbing mechanism 20 grabs each plate and places it on the colony counting device 8. The colony counting device 8 counts the total number of colonies in each plate, thereby calculating the total number of colonies in the 1 ml sample.

[0081] In a further embodiment, Figure 1 As shown, the total bacterial count detection component further includes a third oscillating device 14. The third oscillating device 14 can be a common laboratory oscillator, and its structure and principle are not described in detail.

[0082] The third oscillating device 14 is provided on the platform 1 . After the culture medium container is placed on the third oscillating device 14 , the third oscillating device 14 is started to oscillate and shake the culture medium container to evenly shake the culture medium in the culture medium container.

[0083] With this arrangement, before the pipette 19 aspirates the culture medium, the gripping mechanism 20 is used to grab the culture medium container and place it in the third oscillating device 14. The third oscillating device 14 is used to shake the culture medium in the culture medium container, and after the shaking is even, the gripping mechanism 20 is used to grab the culture medium container and place it in the culture medium area 4. The culture medium is then aspirated using the pipette 19, ensuring a more uniform aspirated culture medium.

[0084] In a further embodiment, Figures 1 to 3 As shown, the total bacterial count detection component further includes a plate shaking mechanism 22. The plate shaking mechanism 22 can be a commonly used plate shaking machine, and its structure and principle are not described in detail.

[0085] The plate shaker is arranged on the platform 1. After the plate is placed on the plate shaker, the plate shaker is started to oscillate the plate to evenly shake the liquid in the plate.

[0086] With this arrangement, after the culture medium and sample or sample liquid are discharged into the dish, the grabbing mechanism 20 grabs the dish and transfers it to the dish shaking mechanism 22. The dish shaking mechanism 22 shakes the liquid in the dish evenly to prepare a more uniform test sample. After shaking evenly, the grabbing mechanism 20 grabs the dish and transfers it to the conveying device 7.

[0087] In one embodiment, Figures 1 to 3 As shown, the carrier is a test piece, and a tablet pressing device 17 is provided in the carrier area. The test piece is placed on the tablet pressing device 17. The tablet pressing device 17 can be a commonly used test piece press. Its structure and principle will not be described in detail. After the test liquid is dripped onto the test piece, the tablet pressing device 17 can compress the test piece.

[0088] Thus, 1 ml of sample, 1:10 sample solution, 1:100 sample solution and 1:1000 sample solution are respectively sucked into the corresponding test piece using the pipette 19 , and then the tablet pressing device 17 compresses each test piece.

[0089] After the tableting is completed, the gripping mechanism 20 is used to grab each test piece and place it on the conveying device 7. The conveying device 7 is then used to transport each test piece into the incubator 6. After each test piece is incubated in the incubator 6, the conveying device 7 is used to transport each test piece out of the incubator 6. Finally, the gripping mechanism 20 is used to grab each test piece and place it on the colony counting device 8. The colony counting device 8 is used to count the total number of colonies in each test piece, thereby calculating the total number of colonies in 1 ml of sample.

[0090] In one embodiment, Figures 1 to 3 As shown, the total colony count detection component also includes an acid-base test paper area and a visual mechanism 23. Acid-base test paper is placed in the acid-base test paper area. After the sample is dropped on the acid-base test paper, the acid-base test paper, that is, pH test paper, can detect the acidity and alkalinity of the sample.

[0091] The visual mechanism 23 can be a graphic sensor or a visual camera, which is visually arranged on the platform 1. After the acid-base test paper detects the acid-base value of the sample, the visual mechanism 23 scans the acid-base test paper to determine the acid-base value of the sample.

[0092] Before drawing a sample, use the pipette 19 to draw 1 ml of sample and drop it onto the acid-base test paper in the acid-base test paper area. The test paper is scanned using the visual mechanism 23, allowing the operator to determine whether the sample's pH value is within the acceptable range. If the sample's pH value is acceptable, the sample can be drawn normally and subsequent operations can be carried out. If the sample's pH value is unacceptable, the operator manually adjusts the sample's pH value to bring it within the acceptable range. The sample can then be drawn normally and subsequent operations can be carried out.

[0093] This setting can ensure in advance that the pH value of the sample meets the requirements, avoiding the failure of subsequent tests due to unqualified pH value of the sample.

[0094] In one embodiment, Figures 1 to 3 As shown, the total bacterial count detection component also includes a waste area 24, which is provided with waste racks for placing discarded test tubes, discarded Erlenmeyer flasks, and discarded petri dishes. Used discarded test tubes, discarded Erlenmeyer flasks, and discarded petri dishes can be placed on the waste racks in the waste area 24.

[0095] In a combined embodiment, the total colony count detection component is used to detect the number of Escherichia coli in a sample.

[0096] First, prepare a homogenous sample solution by multi-level dilution:

[0097] The sample container is grasped by the grasping mechanism 20 and brought to the automatic capping device. During this process, the sample container passes through the first barcode scanning device 9, which scans the first barcode on the sample container and sends it to the LIMS management system for storage. After the container cap of the sample container is opened by the automatic capping device, the grasping mechanism 20 is used to grasp the sample container and bring it to the sample area 2.

[0098] Use the pipette 19 to draw 1 ml of sample from the sample container and drop it on the acid-base test paper in the acid-base test paper area. The operator uses the visual mechanism 23 to determine whether the pH value of the sample is qualified, and adjusts the pH value of the sample until it is qualified if it is unqualified.

[0099] The grabbing mechanism 20 is used to grab the first diluent container containing 225 ml of diluent and bring it to the first oscillating device 12 . After the first oscillating device 12 oscillates and shakes the first diluent container, the grabbing mechanism 20 is used to grab the first diluent container and bring it to the diluent area 3 .

[0100] Use pipette 19 to draw 25 ml of sample from the sample container and then discharge it into the first diluent container. Use gripping mechanism 20 to grab the first diluent container and bring it to first oscillating device 12. Use first oscillating device 12 to shake the liquid in the first diluent container to prepare a 1:10 sample solution. After shaking, use gripping mechanism 20 to grab the first diluent container and return it to diluent area 3.

[0101] The grabbing mechanism 20 is used to grab the first preparation container to the second oscillating device 13. During this process, the sample container passes through the second barcode scanning device 10. The second barcode scanning device 10 scans the second barcode on the preparation container and sends it to the LIMS management system for storage and establishes a corresponding relationship between the sample container and the first preparation container.

[0102] Use pipette 19 to draw 9 ml of saline diluent from the second diluent container and transfer it to the first preparation container. Then, use pipette 19 to draw 1 ml of the 1:10 sample solution from the first diluent container and transfer it to the first preparation container. Use second oscillating device 13 to shake the liquid in the first preparation container, thereby creating a 1:100 sample solution in the first preparation container. After shaking, use gripping mechanism 20 to grab the first preparation container and return it to preparation container area 5.

[0103] The grabbing mechanism 20 is used to grab the second preparation container to the second oscillating device 13. During this process, the sample container passes through the second barcode scanning device 10. The second barcode scanning device 10 scans the second barcode on the preparation container and sends it to the LIMS management system for storage and establishes a correspondence between the sample container and the second preparation container.

[0104] Use pipette 19 to draw 9 ml of the saline diluent from the second diluent container and transfer it to the second preparation container. Then, use pipette 19 to draw 1 ml of the 1:10 sample solution from the first preparation container and transfer it to the second preparation container. Use second oscillating device 13 to shake the liquid in the second preparation container, thereby producing a 1:1000 sample solution in the second preparation container.

[0105] Then, a plate test sample and a test piece test sample were prepared respectively. Specifically, for the plate test sample:

[0106] The culture medium container is grabbed by the grabbing structure and brought to the third oscillating device 14 . After the third oscillating device 14 oscillates and shakes the culture medium container, the culture medium container is grabbed by the grabbing structure and brought to the culture medium area 4 .

[0107] The grabbing structure is used to grab the opened petri dish to the petri dish shaking mechanism 22. During this process, the petri dish passes through the third barcode scanning device 11. The third barcode scanning device 11 scans the third barcode on the petri dish and sends it to the LIMS management system for storage and establishes a correspondence between the sample container and the petri dish.

[0108] First, use the pipette 19 to draw 15ml of culture medium from the culture medium container into the plate, then use the pipette 19 to draw 1ml of sample into the plate. After the plate is covered, the plate is shaken by the plate shaking mechanism 22 to make a plate test sample of the sample stock solution.

[0109] Similarly, prepare 1 ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution, respectively. Two sample solutions can be set for each plate, and a blank plate can also be set for comparison.

[0110] For test piece detection samples:

[0111] The test piece is grabbed by the grabbing structure and placed on the pressing device 17 in the carrier area. During this process, the test piece passes through the third barcode scanning device 11. The third barcode scanning device 11 scans the third barcode on the test piece and sends it to the LIMS management system for storage and establishes a correspondence between the sample container and the test piece.

[0112] Use a pipette 19 to draw 1 ml of sample and drop it onto the test piece. The test piece is flattened by the tablet pressing device 17 to prepare a test piece test sample of the sample stock solution. Similarly, test pieces of 1 ml of sample, 1:10 sample solution, 1:100 sample solution, and 1:1000 sample solution are prepared. Each test piece can be set up with two test samples, and a blank test piece can also be set up for comparison.

[0113] Finally, perform the test:

[0114] The grabbing mechanism 20 grabs the plate sample and the test piece sample and transfers them to the conveyor 7. The conveyor 7 then delivers them to the incubator 6 for incubation. After incubation, the conveyor 7 delivers them out of the incubator 6. The grabbing mechanism 20 then grabs the plate sample and the test piece sample and transfers them to the colony counting device 8. The colony counting device 8 counts the total number of E. coli in each plate sample and test piece sample, thereby calculating the total number of E. coli in 1 ml of the sample.

[0115] According to an embodiment of the present application, on the other hand, a bioassay system is also provided, including any of the above total colony count detection components. The technical effects brought by the system are consistent with those of the total colony count detection component, so they will not be described in detail.

[0116] Although the embodiments of the present application are described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present application, and such modifications and variations shall fall within the scope defined by the present application.

Claims

1. A total bacterial count detection component, characterized in that: The invention comprises a sample area (2) suitable for placing a sample container, a diluent area (3) suitable for placing a diluent container, a preparation container area (5) suitable for placing a preparation container, a carrier area suitable for placing a carrier, an incubator (6), a conveying device (7) and a colony counting device (8) suitable for detecting the total number of colonies, which are respectively arranged on a platform (1), wherein: A space moving device (21) is provided on the platform (1), the space moving device (21) is connected to a gripping mechanism (20) and a liquid transfer gun (19), and the space moving device (21) is suitable for driving the gripping mechanism (20) and the liquid transfer gun (19) to move in the space above the platform (1); The gripping mechanism (20) is suitable for gripping the sample container, the diluent container, the preparation container or the carrier; The pipette (19) is suitable for sucking a sample from the sample container, sucking a diluent from the diluent container, discharging the sucked liquid into the diluent container, sucking a liquid from the preparation container, discharging the sucked liquid into the preparation container, or discharging the sucked liquid into the carrier; The conveying device (7) is suitable for placing the carrier and driving the carrier in and out of the incubator (6).

2. The total bacterial count detection component according to claim 1, characterized in that: Also includes: A first barcode scanning device (9), disposed on the platform (1) and adapted to scan a first barcode on the sample container; A second barcode scanning device (10), disposed on the platform (1) and adapted to scan a second barcode on the preparation container; A third barcode scanning device (11) is arranged on the platform (1) and is suitable for scanning a third barcode on the carrier.

3. The total bacterial count detection component according to claim 1, characterized in that: Also includes: a first oscillating device (12), arranged on the platform (1) and suitable for placing the diluent container, wherein the first oscillating device (12) is capable of shaking the diluent container; A second oscillating device (13) is arranged on the platform (1) and is suitable for placing the preparation container. The second oscillating device (13) can shake the preparation container.

4. The total bacterial count detection component according to claim 1, characterized in that: Also includes: An automatic capping machine (15) is arranged on the platform (1), and the automatic capping machine (15) is suitable for placing a sample container and opening the cap of the sample container.

5. The total bacterial count detection component according to claim 1, characterized in that: The carrier is a petri dish, the carrier area is provided with a petri dish rack (18) for placing the petri dish, the platform (1) is provided with a culture medium area (4) suitable for placing a culture medium container, the grasping mechanism (20) is also suitable for grasping the culture medium container, and the pipette (19) is suitable for sucking culture medium from the culture medium container.

6. The total bacterial count detection component according to claim 5, characterized in that: Also includes: A third oscillating device (14) is arranged on the platform (1) and is suitable for placing the culture medium container. The third oscillating device (14) can shake the culture medium container.

7. The total bacterial count detection component according to claim 5, characterized in that: Also includes: A plate shaking mechanism (22) is provided on the platform (1) and is suitable for placing the plate. The plate shaking mechanism (22) can shake the plate to make it uniform.

8. The total bacterial count detection component according to claim 1, characterized in that: The carrier is a test piece, and the carrier area is provided with a tablet pressing device (17) for placing the test piece.

9. The total bacterial count detection component according to claim 1, characterized in that: Also includes: an acid-base test paper area, arranged on the platform (1) and suitable for placing acid-base test paper; A visual mechanism (23) is provided on the platform (1) and is suitable for scanning and detecting the acid-base test paper.

10. A bioassay system, characterized in that: include: The total colony count detection component according to any one of claims 1 to 9.

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