Immune cell counting device

Abstract

The utility model relates to the field of cell analysis equipment, and discloses an immune cell counting device, which comprises a counting plate for counting and measuring immune cells; the connecting frame is arranged on the counting plate; the auxiliary plate is installed on the connecting frame and used for assisting counting, and the auxiliary plate is arranged to be capable of moving on the connecting frame; the auxiliary plate comprises an auxiliary block, a moving hole is formed in the auxiliary plate, the auxiliary block is connected into the moving hole, and the auxiliary block is arranged to be capable of moving in the moving hole. According to the utility model, through the design of the auxiliary blocks and the moving holes on the auxiliary plate and the combination of the reading holes, the accurate limitation of different areas on the counting plate is realized, the visual relation between cells in a target area and surrounding cells is effectively isolated through the design of the reading holes, the interference of the surrounding cells on the counting process is reduced, and the counting accuracy is improved. And the concentration degree and the accuracy of counting are improved.

Description

Technical Field

[0001] The utility model relates to the field of cell analysis equipment, in particular to an immune cell counting device. Background Art

[0002] Immune cells are the fundamental building blocks of the immune system, including various types such as white blood cells, lymphocytes, macrophages, and natural killer cells. These cells play an irreplaceable role in resisting infection, clearing senescent cells, and monitoring and killing abnormal cells (such as cancer cells). By accurately measuring the number and proportion of these cells, immune cell counting can assess the overall function and status of the immune system, and ultimately determine whether the immune system is functioning normally.

[0003] Hemocytometer counting, as a classic and widely used method of cell counting, relies on observing the number of microorganisms or cells in small squares on a hemocytometer under a microscope.

[0004] Existing counting devices are prone to problems of missed counting and repeated counting. When observing the same small square for a long time, there are many cells in the small square, which is prone to counting errors. The surrounding cells are prone to interference, resulting in missed counting or repeated counting. Utility Model Content

[0005] In order to solve the above-mentioned problems, the present invention is implemented through the following technical solutions:

[0006] An immune cell counting device comprises: a counting plate for performing immune cell counting and cell measurement; a connecting frame, arranged on the counting plate; an auxiliary plate, mounted on the connecting frame, for assisting in counting, the auxiliary plate being configured to be movable on the connecting frame; the auxiliary plate comprising: an auxiliary block, a movable hole being formed on the auxiliary plate, the auxiliary block being connected in the movable hole, the auxiliary block being configured to be movable in the movable hole; a reading hole, formed on the auxiliary block, for defining a visible area through which the immune cells on the counting plate can be accurately counted; by moving the auxiliary plate and the auxiliary block, different areas on the counting plate can be counted one by one or continuously; a second screw rod, connected in the movable hole, the second screw rod passing through the auxiliary block, the second screw rod being used to control the movement of the auxiliary block in the movable hole when rotating; a second adjusting wheel, mounted on the auxiliary plate, one end of the second screw rod being connected to the second adjusting wheel, the second adjusting wheel being used to drive the second screw rod to rotate when rotating on the auxiliary plate.

[0007] The connecting frame includes: a glass slide, mounted on the connecting frame, for carrying the immune cell sample to be counted; a movable groove, opened on the connecting frame; a movable block, connected in the movable groove, and the auxiliary plate is connected to the movable block; a first screw rod, mounted in the movable groove, the first screw rod passes through the movable hole, and the first screw rod is used to control the movement of the movable block in the movable groove when rotating; a first adjusting wheel, mounted on the connecting frame, one end of the first screw rod is connected to the first adjusting wheel, and the first adjusting wheel is used to drive the first screw rod to rotate when rotating on the connecting frame.

[0008] It also includes: a counting platform for providing a counting environment, the counting board is arranged on the counting platform; a counter is arranged on the counting platform for recording the counting process.

[0009] It also includes: a positioning mechanism, which is arranged on the counting board and connected to the connecting frame, and is used to fix the position of the connecting frame on the counting board.

[0010] The positioning mechanism includes: a positioning seat, installed on the counting plate; a limit plate, installed on the positioning seat, the limit plate contacts the connecting frame and is used to limit the position of the connecting frame; an elastic member, one end of which is connected to the positioning seat and the other end is connected to the limit plate, the elastic member is used to provide a restoring force for the initial position of the limit plate.

[0011] The cross-section of the positioning seat is an I-shape.

[0012] The present invention provides an immune cell counting device. Compared with the prior art, it has the following beneficial effects:

[0013] 1. Through the auxiliary block and its movable hole design on the auxiliary plate, combined with the use of the reading hole, accurate definition of different areas on the counting plate is achieved. Only cells in the reading hole are counted each time, effectively avoiding the problems of missed counts and repeated counts caused by too large a field of view or frequent movement of the line of sight. Users can clearly see the cells in the reading hole and count different areas one by one or continuously, improving the accuracy and reliability of counting.

[0014] 2. The design of the reading hole effectively isolates the visual connection between cells in the target area and surrounding cells, reducing the interference of surrounding cells on the counting process. Users can focus on the cells in the reading hole, avoiding omissions or errors in counting caused by dense or similar morphology of surrounding cells, and improving the concentration and accuracy of counting.

[0015] 3. The positioning mechanism can fix the position of the connection frame on the counting board, reducing the displacement error caused by vibration or accidental touch and improving the stability during the counting process. The stable counting environment helps to reduce counting errors caused by improper operation or equipment movement. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the three-dimensional structure proposed by the utility model.

[0017] Figure 2 This is a structural diagram of the counting board, connecting frame, positioning mechanism and auxiliary board proposed in the utility model.

[0018] Figure 3 This is a structural schematic diagram from another perspective of the counting board, connecting frame, positioning mechanism and auxiliary board proposed in the utility model.

[0019] Figure 4 This is a schematic diagram of the positioning mechanism structure proposed by the utility model.

[0020] Figure 5 This is a schematic diagram of the auxiliary block and reading hole structure proposed in the utility model.

[0021] The reference numerals in the figures are:

[0022] 1. Counting table;

[0023] 2. Counting board;

[0024] 3. Connecting frame; 301. Slide; 302. Moving slot; 303. Moving block; 304. First adjusting wheel; 305. First screw rod;

[0025] 4. Positioning mechanism; 401. Positioning seat; 402. Limiting plate; 403. Elastic member;

[0026] 5. Auxiliary plate; 501. Auxiliary block; 502. Moving hole; 503. Second adjusting wheel; 504. Second screw rod; 505. Reading hole;

[0027] 6. Counter. DETAILED DESCRIPTION

[0028] The present invention will be further described below with reference to specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of protection of the present invention.

[0029] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different perspectives and applications without departing from the spirit of the present invention.

[0030] Reference Figure 1-Figure 5, an immune cell counting device, comprising: a counting plate 2, for performing immune cell counting and cell measurement, as a basic platform for counting and measurement, the counting plate 2 provides a precise and stable surface, ensures the accuracy and repeatability of immune cell counting, takes into account the uniformity of cell distribution and the characteristics of easy observation, and lays a solid foundation for subsequent counting and measurement work; a connecting frame 3, arranged on the counting plate 2, the connecting frame 3 serves as a bridge between the auxiliary plate 5 and the counting plate 2, not only firmly supports the auxiliary plate 5, but also enables the auxiliary plate 5 to be flexibly moved on the counting plate 2 through its design, which not only ensures the stability of the structure, but also improves the flexibility and efficiency of counting; the auxiliary plate 5, installed on the connecting frame 3, for auxiliary counting, the auxiliary plate 5 is configured to be movable on the connecting frame 3; the auxiliary plate 5 comprises: an auxiliary block 501, a movable hole 502 is opened on the auxiliary plate 5, the auxiliary block 501 is connected in the movable hole 502, the auxiliary block 501 is configured to be movable in the movable hole 502, the auxiliary plate 5 and its The auxiliary block 501 is cleverly designed. Through the combination of the movable hole 502 and the reading hole 505, accurate counting of different areas on the counting plate 2 is achieved. The mobility of the auxiliary block 501 allows the user to easily adjust the visible area and count cells one by one or continuously, greatly improving the accuracy and speed of counting; the reading hole 505 is formed on the auxiliary block 501 and is used to define a visible area through which the immune cells on the counting plate 2 can be accurately counted; by moving the auxiliary plate 5 and the auxiliary block 501, different areas on the counting plate 2 can be counted one by one or continuously; the second screw rod 504 is connected in the movable hole 502, and the second screw rod 504 passes through the auxiliary block 501. The second screw rod 504 is used to control the movement of the auxiliary block 501 in the movable hole 502 when rotating; the second adjusting wheel 503 is installed on the auxiliary plate 5, and one end of the second screw rod 504 is connected to the second adjusting wheel 503. The second adjusting wheel 503 is used to drive the second screw rod 504 to rotate when rotating on the auxiliary plate 5.

[0031] By limiting the observation field of view to the reading hole 505, it is possible to focus on the cells in this area, reducing counting errors caused by an excessively large field of view or frequent movement of the line of sight, and helping to maintain the accuracy and consistency of the counting; because the reading hole 505 provides a fixed, movable field of view, the new cell area can be clearly seen after each movement of the reading hole 505, avoiding missed counts due to cell overlap or blurred vision, and since only the cells in the reading hole 505 are counted each time, the possibility of repeated counting is also reduced; by quickly moving the reading hole 505 and counting the cells in each area, the cell area on the entire counting plate 2 can be covered more efficiently, which not only improves the counting speed, but also reduces visual fatigue and distraction caused by observing the same area for a long time; the design of the reading hole 505 effectively isolates the visual connection between the cells in the target area and the surrounding cells, reduces the interference of the surrounding cells on the counting process, and can more accurately identify and count the cells in the target area.

[0032] Reference Figure 2 and Figure 3 The connecting frame 3 includes: a glass slide 301, which is installed on the connecting frame 3 and is used to carry the immune cell sample to be counted. The glass slide 301 is used as a carrier for carrying the immune cell sample. Its flatness and smoothness are crucial to the observation effect. By being installed on the connecting frame 3, the glass slide 301 can firmly display the cell sample and provide a clear field of view for counting; a moving groove 302, which is opened on the connecting frame 3; a moving block 303, which is connected in the moving groove 302, and the auxiliary plate 5 is connected to the moving block 303; a first screw rod 305, which is installed in the moving groove 302, and the first screw rod 305 passes through the moving hole 502, and the first screw rod 305 is used to control the movement of the moving block 303 in the moving groove 302 when rotating; a first adjusting wheel 304, which is installed on the connecting frame 3, and one end of the first screw rod 305 is connected to the first adjusting wheel 304, and the first adjusting wheel 304 is used to drive the first screw rod 305 to rotate when rotating on the connecting frame 3.

[0033] Reference Figure 1 and Figure 2 , the counting table 1 is used to provide a counting environment, and the counting board 2 is set on the counting table 1; the counter 6 is set on the counting table 1 and is used to record the counting process. The counting table 1 provides a stable working environment for the entire counting process, and the counter 6 performs counting. The accuracy and efficiency of the counter 6 ensure the reliability and timeliness of the counting results; the positioning mechanism 4 is set on the counting board 2 and connected to the connecting frame 3, and is used to fix the position of the connecting frame 3 on the counting board 2.

[0034] Reference Figure 2 and Figure 4The positioning mechanism 4 includes: a positioning seat 401, which is installed on the counting board 2; a limiting plate 402, which is installed on the positioning seat 401, and the limiting plate 402 is in contact with the connecting frame 3 for limiting the position of the connecting frame 3; an elastic member 403, one end of which is connected to the positioning seat 401, and the other end is connected to the limiting plate 402, and the elastic member 403 is used to provide a restoring force for the initial position of the limiting plate 402; the cross-sectional shape of the positioning seat 401 is I-shaped, and the positioning mechanism 4 firmly fixes the position of the connecting frame 3 on the counting board 2 through the combination of the positioning seat 401, the limiting plate 402 and the elastic member 403, which not only improves the stability during the counting process, but also reduces the displacement error caused by vibration or mistouch. The I-shaped cross-sectional design of the positioning seat 401 can provide moving space for the limiting plate 402, thereby improving the reliability of positioning. The elastic member 403 adopts a stainless steel spring or a rubber elastic block.

[0035] During use, the immune cell sample to be counted is placed on the slide 301, and the slide 301 is firmly installed on the connecting frame 3 to ensure that the slide 301 is flat and fixed in position. The limiting plate 402 is pulled, and the limiting plate 402 pulls the elastic member 403 to place the connecting frame 3 on the counting board 2. After loosening the limiting plate 402, the elastic member 403 can drive the limiting plate 402 to reset and fix the position of the connecting frame 3 to prevent displacement during the counting process. The first adjusting wheel 304 is rotated to drive the moving block 303 to move in the moving groove 302 through the first screw rod 305, and the initial position of the auxiliary plate 5 on the connecting frame 3 is adjusted. The immune cells in the area are observed and counted through the reading hole 505 on the auxiliary block 501. The design of the reading hole 505 limits the observation field of view, which helps to reduce counting errors and duplication. Re-counting: After completing the counting of one area, rotate the second adjusting wheel 503, and control the auxiliary block 501 to move in the moving hole 502 through the second screw rod 504, change the position of the reading hole 505, and align it with the next area to be counted. Repeat the above steps, count different areas on the counting plate 2 one by one or continuously until the entire target counting area is covered, and use the counter 6 set on the counting table 1 to record the counting process. The counter 6 can record the number of cells. The accuracy and efficiency of the counter 6 ensure the reliability and timeliness of the counting results and reduce human errors. After completing the counting, turn off the counter 6 and disconnect the power supply. Carefully remove the slide 301 from the connecting frame 3, and properly dispose of the counted immune cell sample. Clean the counting plate 2, connecting frame 3, auxiliary plate 5 and other components for next use.

[0036] In summary, compared with the existing technology, it has the following beneficial effects:

[0037] Through the design of the auxiliary block 501 and the movable hole 502 on the auxiliary plate 5, combined with the use of the reading hole 505, accurate definition of different areas on the counting plate 2 is achieved, and only the cells in the reading hole 505 are counted each time, effectively avoiding the problems of missed counting and repeated counting caused by too large a field of view or frequent movement of the line of sight. The user can clearly see the cells in the reading hole 505, count different areas one by one or continuously, and improve the accuracy and reliability of the counting.

[0038] The design of the reading hole 505 effectively isolates the visual connection between the cells in the target area and the surrounding cells, reducing the interference of the surrounding cells on the counting process. The user can focus on the cells in the reading hole 505, avoiding omissions or errors in counting due to dense or similar morphology of surrounding cells, and improving the concentration and accuracy of counting.

[0039] The position of the connecting frame 3 on the counting board 2 can be fixed by the positioning mechanism 4, which reduces the displacement error caused by vibration or accidental touch and improves the stability during the counting process. The stable counting environment helps to reduce counting errors caused by improper operation or equipment movement.

[0040] Thus, although the present invention has been described herein with reference to specific embodiments thereof, freedom of modification, various changes and substitutions are within the foregoing disclosure, and it should be understood that in some cases, some features of the present invention will be employed without the corresponding use of other features without departing from the scope and spirit of the proposed invention. Thus, many modifications may be made to adapt particular circumstances or materials to the true scope and spirit of the present invention. The present invention is not intended to be limited to the specific terminology used in the claims below and / or to the specific embodiments disclosed as the best mode contemplated for carrying out the invention, but the present invention will include any and all embodiments and equivalents falling within the scope of the appended claims. Therefore, the scope of the present invention will be determined solely by the appended claims.

Claims

1. An immune cell counting device, characterized in that: include: Counting chamber (2), used for immune cell counting and cell measurement; A connecting frame (3) is arranged on the counting board (2); An auxiliary plate (5) is mounted on the connecting frame (3) and is used to assist in counting. The auxiliary plate (5) is configured to be movable on the connecting frame (3); The auxiliary plate (5) comprises: An auxiliary block (501), wherein a movable hole (502) is provided on the auxiliary plate (5), and the auxiliary block (501) is connected in the movable hole (502), and the auxiliary block (501) is configured to be movable in the movable hole (502); The reading hole (505) is formed on the auxiliary block (501) and is used to define a visible area through which the immune cells on the counting plate (2) can be accurately counted; by moving the auxiliary plate (5) and the auxiliary block (501), different areas on the counting plate (2) can be counted one by one or continuously.

2. The immune cell counting device according to claim 1, characterized in that: The auxiliary plate (5) further comprises: a second screw rod (504) connected to the movable hole (502), the second screw rod (504) passing through the auxiliary block (501), and the second screw rod (504) being used to control the auxiliary block (501) to move in the movable hole (502) when rotating; The second adjusting wheel (503) is mounted on the auxiliary plate (5), and one end of the second screw rod (504) is connected to the second adjusting wheel (503). The second adjusting wheel (503) is used to drive the second screw rod (504) to rotate when rotating on the auxiliary plate (5).

3. The immune cell counting device according to claim 1, characterized in that: The connection frame (3) includes: A glass slide (301) is mounted on the connection frame (3) and is used to carry immune cell samples to be counted.

4. The immune cell counting device according to claim 3, characterized in that: The connection frame (3) further includes: A movable slot (302) is provided on the connecting frame (3); A moving block (303) is connected in the moving groove (302), and the auxiliary plate (5) is connected to the moving block (303); A first screw rod (305) is installed in the movable groove (302), and the first screw rod (305) passes through the movable hole (502). The first screw rod (305) is used to control the movable block (303) to move in the movable groove (302) when rotating; The first adjusting wheel (304) is mounted on the connecting frame (3), one end of the first screw rod (305) is connected to the first adjusting wheel (304), and the first adjusting wheel (304) is used to drive the first screw rod (305) to rotate when rotating on the connecting frame (3).

5. The immune cell counting device according to claim 1, characterized in that: Also includes: A counting table (1) is used to provide a counting environment, and the counting board (2) is arranged on the counting table (1); A counter (6) is provided on the counting platform (1) and is used to record the counting process.

6. The immune cell counting device according to claim 1, characterized in that: Also includes: A positioning mechanism (4) is provided on the counting board (2), connected to the connecting frame (3), and is used to fix the position of the connecting frame (3) on the counting board (2).

7. The immune cell counting device according to claim 6, characterized in that: The positioning mechanism (4) comprises: A positioning seat (401) is mounted on the counting board (2); A limiting plate (402) is mounted on the positioning seat (401), the limiting plate (402) being in contact with the connecting frame (3) and used to limit the position of the connecting frame (3); The elastic member (403) has one end connected to the positioning seat (401) and the other end connected to the limiting plate (402). The elastic member (403) is used to provide a restoring force for the limiting plate (402) to maintain its initial position.

8. The immune cell counting device according to claim 7, characterized in that: The cross-sectional shape of the positioning seat (401) is an I-shape.

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