Protein glue dye liquor collecting device
By designing an automated protein gel staining solution collection device, the problems of uneven irradiation, cumbersome operation and time-consuming process in the preparation and collection of protein gel staining solution were solved, and an efficient and simplified operation process and uniform stirring effect were achieved.
Patent Information
- Application Number
- CN202422621028.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-29
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2034-10-29
AI Technical Summary
The existing preparation and collection process of protein gel staining solution has problems such as uneven irradiation, complicated operation and long time consumption.
A protein gel staining solution collection device was designed, which was equipped with automatic UV lamp irradiation, stirring components and temperature control functions to achieve automatic stirring and temperature regulation, and has automatic collection function.
The invention improves the preparation efficiency of the protein gel staining solution, reduces the difficulty of operation, simplifies the operation process, and improves the stirring uniformity and the accuracy of temperature control.
Smart Images

Figure CN223413070U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of dye solution collection, in particular to a device for collecting protein gel dye solution. Background Art
[0002] Protein gel staining solution uses Coomassie Brilliant Blue as the dye. It is fast, efficient, pollution-free, highly sensitive, and easy to use. It can be used to stain protein gels or detect residual protein after transfer. Protein gel staining solution is a non-toxic, odorless, and highly environmentally friendly staining solution. Ordinary conventional methods require the use of highly toxic methanol and highly irritating acetic acid.
[0003] However, protein gel staining solutions are usually prepared on-site, which can lead to uneven irradiation and cumbersome operation when using a handheld UV lamp during protein detection. Stirring is also required, resulting in a long time required for overall use or collection. Utility Model Content
[0004] (1) Technical problems solved
[0005] In response to the shortcomings of the existing technology, the utility model provides a protein gel dye collection device with automatic ultraviolet light irradiation, convenient continuous stirring and temperature control effects, and convenient collection during use, which improves the overall preparation efficiency and reduces the difficulty of operation, solving the above technical problems.
[0006] (2) Technical solution
[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a protein gel staining liquid collection device, comprising a base, a mounting seat fixedly mounted on the left end of the top side of the base, a protective cylinder provided on the inner side of the mounting seat, a reaction dish fixedly mounted on the inner side of the protective cylinder, a movable frame movably mounted on the top side of the mounting seat, a stirring assembly provided on the inner side of the reaction dish, a pump connected to the bottom side of the reaction dish via a pipe, a water outlet end of the pump connected to a collecting cylinder via a pipe, and a collecting dish movably mounted on the inner side of the collecting cylinder.
[0008] Preferably, heating lamps are provided on the inner side of the protective tube. There are three groups of heating lamps, which are arranged in a circular and equidistant manner on the inner side of the protective tube. The reaction vessel is provided with a heat conducting plate, which is a circular structure.
[0009] Through the above technical solution, the power of the heating lamp is turned on during the preparation process, so that the temperature can be adjusted easily. At this time, the heat is stably conducted under the action of the heat conduction plate, thereby facilitating the adjustment of the appropriate temperature.
[0010] Preferably, a connecting sleeve is fixedly installed on the upper end of the outer side of the mounting seat, a sliding groove is provided on the outer side of the connecting sleeve, a slider is provided on the inner side of the sliding groove, and a moving frame is fixedly installed on the outermost side of the slider, and an electric push rod is fixedly installed between the moving frame and the mounting seat.
[0011] Through the above technical solution, during the downward movement of the mobile rack, the cooperation of the slide groove and the slider is conducive to improving the stability of the mobile rack during downward movement, and the electric push rod is conducive to achieving automatic covering.
[0012] Preferably, a sealing cover is fixedly installed on the bottom side of the movable frame, and a plurality of groups of ultraviolet lamps are evenly arranged and installed on the bottom side of the sealing cover. The size and shape of the sealing cover match the through hole on the top side of the protective tube.
[0013] Through the above technical solution, the electric push rod is started during use to drive the movable frame to move downward, thereby engaging the sealing cover with the protective tube, making it convenient to connect the ultraviolet lamp power supply for ultraviolet irradiation later, solving the need for manual hand-held irradiation process during use and reducing the overall operation difficulty.
[0014] Preferably, the stirring assembly includes a servo motor, a connecting shaft and a stirring rod, the driving end of the servo motor is fixedly mounted with the connecting shaft, the top end of the connecting shaft is fixedly mounted with the stirring rod, and the stirring rod is movably mounted on the inner side of the reaction vessel.
[0015] Through the above technical solution, during preparation, the servo motor is started to drive the connecting shaft and the stirring rod to rotate at a low speed, thereby facilitating continuous stirring during the preparation process, solving the need for manual stirring during the preparation process, and improving the stirring uniformity and continuity.
[0016] Preferably, a ferrule is fixedly installed on the inner side of the collecting cylinder, and the ferrule is a circular structure. A telescopic hose is provided on the left side of the inner side of the collecting cylinder, and the left end of the telescopic hose is connected to the water outlet end of the pump through a pipeline.
[0017] Through the above technical solution, during the collection process, the liquid flow direction can be conveniently adjusted by rotating the telescopic hose, and the collection dish can be conveniently disassembled and assembled through the card sleeve, which is easy to use and has high collection efficiency.
[0018] Preferably, a fitting sleeve is fixedly mounted on the bottom side of the collecting dish, the size and shape of the fitting sleeve match the slot on the top side of the ferrule, and a circular collecting cavity is provided on the top side of the collecting dish.
[0019] Through the above technical solution, after the reaction is complete, the pump is started to collect the liquid in the reaction dish to the inner side of the collection dish, so that it is convenient to take it out to check the reaction effect, and it is convenient to engage with the card sleeve under the action of the fitting sleeve.
[0020] Compared with the prior art, the present invention provides a device for collecting protein gel staining liquid, which has the following beneficial effects:
[0021] 1. The utility model places the gel and staining solution into the inner side of the reaction vessel respectively, and then starts the electric push rod to drive the movable frame downward, thereby engaging the sealing cover with the protective tube, thereby facilitating the subsequent connection of the UV lamp power supply for UV irradiation, eliminating the need for manual hand-held irradiation during use and reducing the overall difficulty of operation;
[0022] 2. The utility model facilitates temperature adjustment by turning on the power of the heating lamp during the preparation process. At this time, the heat is stably conducted under the action of the heat conducting plate, thereby facilitating adjustment of the appropriate temperature. Then, the servo motor is started to drive the connecting shaft and the stirring rod to rotate at a low speed, thereby facilitating continuous stirring during the preparation process, solving the need for manual stirring during the preparation process, and improving stirring uniformity and continuity.
[0023] 3. After the reaction is complete, the utility model starts the pump to collect the liquid in the reaction dish to the inner side of the collection dish, which is convenient for taking out to check the reaction effect. At the same time, the liquid flow direction is conveniently adjusted through the telescopic hose, and the collection dish is conveniently disassembled and assembled through the card sleeve. It is easy and quick to use, and the overall operation difficulty is low. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of the utility model;
[0025] Figure 2 This is a schematic diagram of the cross-sectional structure of the utility model;
[0026] Figure 3 For this utility model Figure 2 A in the figure shows the enlarged structural diagram;
[0027] Figure 4 This is a schematic diagram of the three-dimensional structure of the collection dish of the utility model;
[0028] Figure 5 This is a schematic diagram of the structure of the mobile rack of the utility model when viewed from above.
[0029] Among them: 1. Base; 2. Mounting seat; 3. Protective tube; 4. Reaction dish; 5. Mobile rack; 6. Stirring assembly; 7. Pump; 8. Collection tube; 9. Collection dish; 301. Heating lamp; 401. Heat conduction plate; 201. Connecting sleeve; 202. Slide groove; 203. Slider; 204. Electric push rod; 501. Sealing cover; 502. UV lamp; 601. Servo motor; 602. Connecting shaft; 603. Stirring rod; 801. Card sleeve; 802. Telescopic hose; 901. Fitting sleeve. DETAILED DESCRIPTION
[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0031] Example 1:
[0032] like Figure 1-5 As shown, the utility model provides a protein gel staining liquid collection device, which includes a base 1, a mounting seat 2 is fixedly installed on the left end of the top side of the base 1, a protective cylinder 3 is provided on the inner side of the mounting seat 2, a reaction dish 4 is fixedly installed on the inner side of the protective cylinder 3, a movable frame 5 is movably installed on the top side of the mounting seat 2, a stirring assembly 6 is provided on the inner side of the reaction dish 4, the bottom side of the reaction dish 4 is connected to a pump 7 through a pipeline, the water outlet end of the pump 7 is connected to a collecting cylinder 8 through a pipeline, and a collecting dish 9 is movably installed on the inner side of the collecting cylinder 8.
[0033] Specifically, three sets of heating lamps 301 are installed inside the protective tube 3, arranged equidistantly in a circular pattern. The reaction vessel 4 is equipped with a circular heat conducting plate 401. This facilitates temperature adjustment by simply powering on the heating lamps 301. Heat is then stably conducted through the heat conducting plate 401, allowing for easy adjustment to a desired temperature.
[0034] Specifically, a connecting sleeve 201 is fixedly mounted on the upper end of the outer side of the mounting base 2. A sliding groove 202 is formed on the outer side of the connecting sleeve 201. A slider 203 is provided on the inner side of the sliding groove 202. The outermost side of the slider 203 is fixedly mounted on the movable frame 5. An electric push rod 204 is fixedly mounted between the movable frame 5 and the mounting base 2. Advantageously, the cooperation between the sliding groove 202 and the slider 203 helps to improve the stability of the movable frame 5 when it moves downward.
[0035] Specifically, a sealing cover 501 is fixedly mounted on the bottom side of the mobile frame 5. Several sets of UV lamps 502 are evenly spaced and mounted on the bottom side of the sealing cover 501. The size and shape of the sealing cover 501 match the through-holes on the top side of the protective tube 3. Advantageously, the sealing cover 501 engages the protective tube 3, making it easier to connect the UV lamps 502 for UV irradiation. This eliminates the need for manual hand-held irradiation during use and reduces overall operational difficulty.
[0036] Example 2:
[0037] like Figure 1-5As shown, as an improvement to the previous embodiment, the stirring assembly 6 includes a servo motor 601, a connecting shaft 602, and a stirring rod 603. The driving end of the servo motor 601 is fixedly mounted with the connecting shaft 602, and the top end of the connecting shaft 602 is fixedly mounted with the stirring rod 603. The stirring rod 603 is movably mounted on the inner side of the reaction vessel 4. The advantage is that by starting the servo motor 601 to drive the connecting shaft 602 and the stirring rod 603 to rotate at a low speed, continuous stirring is facilitated during the preparation process, eliminating the need for manual stirring during the preparation process and improving stirring uniformity and continuity.
[0038] Specifically, a circular ferrule 801 is fixedly mounted on the inside of the collection tube 8. A telescopic hose 802 is provided on the left side of the inside of the collection tube 8. The left end of the telescopic hose 802 is connected to the water outlet of the pump 7 via a pipe. Advantageously, the telescopic hose 802 facilitates adjustment of the liquid flow direction, while the ferrule 801 facilitates assembly and disassembly of the collection dish 9.
[0039] Specifically, a fitting sleeve 901 is fixedly mounted on the bottom side of the collection dish 9. The size and shape of the fitting sleeve 901 match the slot on the top side of the ferrule 801. A circular collection cavity is defined on the top side of the collection dish 9. Advantageously, by activating the pump 7, the liquid in the reaction dish 4 is collected inside the collection dish 9, making it easy to remove and inspect the reaction results.
[0040] During use, the gel and the staining solution are placed in the inner side of the reaction vessel 4 respectively, and then the electric push rod 204 is started to drive the movable frame 5 to move downward, so that the sealing cover 501 is engaged with the protective tube 3, so as to facilitate the subsequent connection of the ultraviolet lamp 502 power supply for ultraviolet irradiation, which solves the need for manual hand-held irradiation process during use and reduces the overall operation difficulty. In addition, during the downward movement of the movable frame 5, the cooperation of the slide 202 and the slider 203 is conducive to improving the stability of the movable frame 5 when moving downward. Then, during the preparation process, the power of the heating lamp 301 is turned on, so as to facilitate temperature adjustment. At this time, stable conduction is carried out under the action of the heat conduction plate 401, which makes it convenient to adjust the appropriate temperature. Then, by starting the servo motor 601, the connecting shaft 602 and the stirring rod 603 are driven to rotate at a low speed, so as to facilitate continuous stirring during the preparation process, solve the need for manual stirring during the preparation process, and improve the stirring uniformity and continuity. After the reaction is complete, the pump 7 is started to collect the liquid in the reaction dish 4 to the inner side of the collection dish 9, so that it is convenient to take it out to check the reaction effect. At the same time, the telescopic hose 802 is used to facilitate the adjustment of the liquid flow direction, and the card sleeve 801 is used to facilitate the disassembly and assembly of the collection dish 9.
[0041] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A device for collecting protein gel staining solution, comprising a base (1), characterized in that: A mounting seat (2) is fixedly mounted on the left end of the top side of the base (1), a protective cylinder (3) is provided on the inner side of the mounting seat (2), a reaction dish (4) is fixedly mounted on the inner side of the protective cylinder (3), a movable frame (5) is movably mounted on the top side of the mounting seat (2), a stirring assembly (6) is provided on the inner side of the reaction dish (4), a pump (7) is connected to the bottom side of the reaction dish (4) through a pipeline, a water outlet end of the pump (7) is connected to a collecting cylinder (8) through a pipeline, and a collecting dish (9) is movably mounted on the inner side of the collecting cylinder (8).
2. The protein gel staining solution collecting device according to claim 1, characterized in that: A heating lamp (301) is provided on the inner side of the protective tube (3). Three groups of heating lamps (301) are provided and are respectively arranged in a circular manner and equidistantly installed on the inner side of the protective tube (3). The reaction vessel (4) is provided with a heat conducting plate (401), and the heat conducting plate (401) is a circular structure.
3. The protein gel staining solution collecting device according to claim 1, characterized in that: A connecting sleeve (201) is fixedly mounted on the upper end of the outer side of the mounting seat (2), a sliding groove (202) is provided on the outer side of the connecting sleeve (201), a slider (203) is provided on the inner side of the sliding groove (202), and a moving frame (5) is fixedly mounted on the outermost side of the slider (203), and an electric push rod (204) is fixedly mounted between the moving frame (5) and the mounting seat (2).
4. The protein gel staining solution collecting device according to claim 1, characterized in that: A sealing cover (501) is fixedly mounted on the bottom side of the movable frame (5), and a plurality of groups of ultraviolet lamps (502) are evenly spaced and mounted on the bottom side of the sealing cover (501). The size and shape of the sealing cover (501) match the top through hole of the protective tube (3).
5. The protein gel staining solution collecting device according to claim 1, characterized in that: The stirring assembly (6) comprises a servo motor (601), a connecting shaft (602) and a stirring rod (603). The driving end of the servo motor (601) is fixedly mounted with the connecting shaft (602). The top end of the connecting shaft (602) is fixedly mounted with the stirring rod (603). The stirring rod (603) is movably mounted on the inner side of the reaction vessel (4).
6. The protein gel staining solution collecting device according to claim 1, characterized in that: A ferrule (801) is fixedly mounted on the inner side of the collecting cylinder (8), and the ferrule (801) is a circular structure. A telescopic hose (802) is provided on the left side of the inner side of the collecting cylinder (8), and the left end of the telescopic hose (802) is connected to the water outlet of the pump (7) via a pipeline.
7. The protein gel staining solution collecting device according to claim 6, characterized in that: A fitting sleeve (901) is fixedly mounted on the bottom side of the collecting dish (9), the size and shape of the fitting sleeve (901) match the top slot of the ferrule (801), and a circular collecting cavity is provided on the top side of the collecting dish (9).