Preparation device of agarose gel
By designing an agarose gel preparation device with intelligent temperature control and automated stirring, the problems of low agarose gel preparation efficiency and uneven concentration were solved, efficient and stable agarose gel preparation was achieved, and experimental costs were reduced.
Patent Information
- Application Number
- CN202422503197.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-16
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-10-16
AI Technical Summary
In the prior art, agarose gel preparation efficiency is low and temperature control is imprecise, resulting in uneven agarose gel concentration, affecting electrophoresis results, and requiring manual intervention, which increases experimental costs.
An agarose gel preparation device including a temperature control component was designed. It used a large-volume inner tank, a temperature detection element and an intelligent control module to achieve precise temperature control and automatic stirring, and was capable of preparing multiple plates of agarose solutions of different concentrations at one time.
The preparation efficiency and quality of agarose gel are improved, manual intervention is reduced, experimental costs are lowered, and the uniformity and stability of agarose gel are ensured.
Smart Images
Figure CN223417241U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to preparation device technical field, especially a kind of preparation device of agarose gel. BACKGROUND
[0002] Agarose is a linear polysaccharide polymer, and agarose gel is a gel prepared by using agarose as supporting medium. It can be used in biochemical and medical research fields. Low-melting-point agarose with reduced melting point after modification has a melting point of 90℃. After being heated and melted in a microwave oven, it can maintain liquid state for several hours at 50-60℃, and solidify into solid gel when naturally cooled to 36℃.
[0003] After nucleic acid extraction, electrophoresis technology is needed to detect its quantity and quality. Since agarose was introduced into nucleic acid research, agarose gel electrophoresis technology for separating DNA according to its relative molecular mass has developed into an important experimental method for analyzing and identifying DNA molecules. Agarose gel electrophoresis technology can be used for nucleic acid separation, identification and purification.
[0004] In the prior art, microwave ovens are used to dissolve agarose solution, which has limited volume, long preparation time and low preparation efficiency. The prepared agarose solution needs to be naturally cooled and cannot be used immediately. Manual operation and observation of the changes in agarose form and temperature are required. If the temperature of the agarose solution is too high, the nucleic acid dye will volatilize. If the temperature is too low, local solidification will occur in the agarose solution, which will affect the electrophoresis results. In severe cases, the gel needs to be prepared again and electrophoresis is required, which wastes time and increases experimental cost. UTILITY MODEL CONTENT
[0005] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model provides a preparation device for agarose gel, which can realize intelligent and accurate temperature control, improve the preparation quality of agarose gel, and prepare multiple plates of agarose solution with different concentrations at one time, realize the preparation of large quantities of agarose gel, and improve the preparation efficiency.
[0006] The preparation device for agarose gel according to the utility model embodiment comprises a shell, an inner container, a temperature control assembly, and a control module, a temperature detection element, a heating structure and a refrigeration structure. The temperature detection element, the heating structure and the refrigeration structure are electrically connected to the control module. The temperature detection element is used to detect the temperature of the inner container. The control module is adapted to control the heating structure to heat the inner container or control the refrigeration structure to cool the inner container according to the detection value of the temperature detection element.
[0007] According to the agarose gel preparation device of the embodiment of the present invention, a large-volume inner liner is provided to form a preparation space inside the inner liner for holding and storing agarose gel solution, thereby realizing the preparation of large quantities of agarose gel and improving preparation efficiency. A temperature detection element is provided to detect the temperature in real time, and a control module intelligently controls the operation of the heating structure and the cooling structure to achieve intelligent and precise temperature control, thereby improving the preparation quality of agarose gel. In addition, multiple plates of agarose solutions of different concentrations can be prepared at one time, further improving preparation efficiency. No manual intervention is required during the preparation process, thus saving manpower and reducing costs.
[0008] According to the agarose gel preparation device of some embodiments of the present invention, a temperature control space is formed between the inner liner and the shell, and the temperature control component is installed in the temperature control space; wherein the heating structure and the cooling structure are both located at the bottom of the inner liner and spaced apart in the horizontal direction.
[0009] According to the agarose gel preparation device of some embodiments of the present invention, a stirring member is provided in the inner container, and the stirring member is rotatably arranged at the bottom of the inner container. At least a portion of the stirring member is distributed opposite to the heating structure in the up-down direction, and at least another portion of the stirring member is distributed opposite to the cooling structure in the up-down direction.
[0010] According to some embodiments of the agarose gel preparation device of the present invention, the agarose gel preparation device further includes a driving member, which is located in the temperature-controlled space, connected to the stirring member and used to drive the stirring member to rotate.
[0011] According to the agarose gel preparation device in some embodiments of the present invention, the driving member is located between the refrigeration structure and the heating structure.
[0012] According to the agarose gel preparation device of some embodiments of the present invention, a heat-insulating layer is provided on the outer side of the inner container, and the heating structure and the cooling structure respectively conduct heat to the inner container through the heat-insulating layer.
[0013] According to the agarose gel preparation device of some embodiments of the present invention, the refrigeration structure includes a semiconductor refrigeration plate, the cold end of the semiconductor refrigeration plate is used to cool the inner container; and / or, cold energy is transferred between the refrigeration structure and the inner container via a cooling plate.
[0014] According to the device for preparing agarose gel in some embodiments of the present invention, the heating structure includes a PTC heating sheet.
[0015] According to the agarose gel preparation device of some embodiments of the present invention, the refrigeration structure is provided with a heat sink on a side away from the inner container, and / or the heating structure is provided with a heat sink on a side away from the inner container.
[0016] According to the agarose gel preparation device of some embodiments of the present invention, the inner liner includes an inner liner body and an inner liner sealing cover, the inner liner body forms the preparation space open upward, and the inner liner sealing cover is detachably connected to the upper end of the inner liner body to close the preparation space.
[0017] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments in conjunction with the following drawings, in which:
[0019] Figure 1 1 is a schematic structural diagram of an agarose gel preparation device according to an embodiment of the present utility model;
[0020] Figure 2 It is a cross-sectional view of a device for preparing agarose gel according to an embodiment of the present utility model.
[0021] Reference numerals:
[0022] Agarose gel preparation device 100,
[0023] Shell 1, liner 2, liner body 21, preparation space 211, liner sealing cover 22, insulation layer 23,
[0024] PTC heating sheet 311, semiconductor cooling sheet 321,
[0025] Temperature control space 41 , stirring element 5 , driving element 6 , cooling fins 7 , and heat sinks 8 . DETAILED DESCRIPTION
[0026] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.
[0027] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, features defined as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.
[0028] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on the specific circumstances.
[0029] Reference below Figure 1-Figure 2 The present invention provides an agarose gel preparation device 100. The agarose gel preparation device 100 can achieve intelligent and precise temperature control, improve the preparation quality of agarose gel, and prepare multiple plates of agarose solutions of different concentrations at one time, thereby realizing the preparation of large quantities of agarose gel and improving preparation efficiency.
[0030] like Figure 1-Figure 2 As shown, an agarose gel preparation device 100 according to an embodiment of the present invention comprises: a housing 1, an inner container 2 and a temperature control component. It should be noted that the agarose gel preparation device 100 is used to prepare agarose gel.
[0031] The shell 1 is the outer structure of the entire preparation device 100, which is used to protect and support the inner liner 2. An installation space is formed inside the shell 1 so that the inner liner 2 can be installed in the shell 1. A preparation space 211 is formed in the inner liner 2, that is, the inner liner 2 can be constructed into a cylindrical shape, etc., and its interior is hollow to form the preparation space 211. The preparation space 211 can hold and store agarose gel solution and heat or cool it to prepare agarose gel.
[0032] The volume of the inner liner 2 is relatively large. For example, the volume of the inner liner 2 can be 4L, 5L, 6L, 7L, etc., so that the preparation space 211 is increased, and a larger amount of gel solution required for preparing agarose gel can be prepared, thereby realizing the preparation of large quantities of agarose gel and improving the preparation efficiency.
[0033] Furthermore, the temperature control component includes a control module, a temperature detection element, a heating structure and a cooling structure. The temperature detection element, the heating structure and the cooling structure are electrically connected to the control module respectively. The temperature detection element is used to detect the temperature of the inner liner 2. The control module is suitable for controlling the heating structure to heat the inner liner 2 or controlling the cooling structure to cool the inner liner 2 according to the detection value of the temperature detection element.
[0034] Specifically, in the process of preparing agarose gel, different temperature conditions are required at different stages (for example, heating to dissolve agarose and cooling to form a gel in the agarose solution). Therefore, the preparation temperature needs to be precisely controlled. If the temperature of the agarose solution is too high, the nucleic acid dye will volatilize. If the temperature of the agarose solution is too low, local coagulation will occur in the agarose solution, resulting in uneven concentration of the agarose gel and affecting the electrophoresis effect. In severe cases, the gel must be re-prepared and electrophoresis must be repeated, which wastes time and increases experimental costs.
[0035] The temperature control assembly is used to precisely control the temperature within the inner liner 2 to ensure that agarose gel can be prepared at the desired temperature, thereby improving the quality of agarose gel preparation and reducing experimental costs. The control module is the control center of the temperature control assembly. The temperature sensing element, heating structure, and cooling structure are electrically connected to the control module, respectively. That is, the control module can transmit signals to the temperature sensing element, heating structure, and cooling structure. The temperature sensing element is used to detect the temperature of the inner liner 2 in real time. In practice, it can be constructed as a temperature sensor, such as a thermocouple or thermistor, and converts the detected value into an electrical signal and transmits it to the control module. After receiving the signal from the temperature sensing element, the control module can compare the signal with the current detected actual temperature value according to a preset program or condition (such as the target temperature), determine whether the temperature is higher or lower, and then control the operation of the heating structure or cooling structure.
[0036] For example, when the control module determines that the actual temperature of the inner liner 2 is lower than the set temperature, the control module controls the start-up of the heating structure to heat the inner liner 2 and increase the temperature of the inner liner 2. When the control module determines that the actual temperature of the inner liner 2 is higher than the set temperature, the control module controls the start-up of the cooling structure to cool the inner liner 2 and reduce the temperature of the inner liner 2. Thus, through such intelligent cycle control, it can be ensured that the temperature of the inner liner 2 is always maintained within the preset temperature range, thereby meeting the specific temperature preparation requirements of the agarose gel and improving the preparation quality of the agarose gel.
[0037] Thus, the automated temperature control component quickly responds to temperature changes to quickly and accurately adjust the temperature of the inner liner 2, thereby shortening the time and improving the preparation efficiency, realizing intelligent and precise temperature control, and preparing multiple plates of agarose solutions with different concentrations at one time, that is, preparing agarose gels with different required concentrations at one time, further improving the preparation efficiency; and controlling the temperature stably can ensure the temperature stability of the inner liner 2, and the stable temperature environment is conducive to the agarose solution forming a good gel structure, thereby improving the uniformity and quality stability of the agarose gel.
[0038] According to the agarose gel preparation device 100 of the embodiment of the present invention, by providing a large-volume inner liner 2, a preparation space 211 capable of containing and storing agarose gel solution is formed in the inner liner 2, thereby enabling the preparation of large quantities of agarose gel and improving the preparation efficiency. By providing a temperature detection element to detect the temperature in real time, the control module intelligently controls the operation of the heating structure and the cooling structure, thereby achieving intelligent and precise temperature control, improving the preparation quality of agarose gel, and being able to prepare multiple plates of agarose solutions of different concentrations at one time, further improving the preparation efficiency. During the preparation process, no manual intervention is required, saving manpower and reducing costs.
[0039] In some embodiments, a temperature control space 41 is formed between the inner liner 2 and the shell 1 , and the temperature control component is installed in the temperature control space 41 .
[0040] Specifically, if Figure 2 As shown, a certain space is separated between the inner liner 2 and the shell 1 to form a temperature-controlled space 41. The temperature-controlled space 41 can play a role in heat insulation and heat preservation, that is, it reduces the direct heat exchange between the inner liner 2 and the external environment and keeps the temperature of the inner liner 2 stable. At the same time, the temperature control component is installed in the temperature-controlled space 41, that is, the temperature-controlled space 41 provides a certain protection for the temperature control component to prevent it from being exposed to the external environment to cause damage. The temperature-controlled space 41 can effectively transfer the heat generated by the temperature control component to the inner liner 2.
[0041] The heating structure and the cooling structure are both located at the bottom of the inner container 2 and are spaced apart in the horizontal direction.
[0042] Specifically, the heating structure and the cooling structure are both arranged at the bottom of the inner liner 2 and installed in the temperature-controlled space 41. In this way, the heat generated by the heating structure and the cold generated by the cooling structure are transferred from the bottom to the top, so that the agarose solution in the inner liner 2, that is, the preparation space 211, can be uniformly heated or cooled, reducing the generation of temperature gradients.
[0043] The heating structure and the cooling structure are spaced apart horizontally, for example, they can be spaced apart in the left-right direction and the front-back direction, with a certain distance between them. In this way, mutual interference between the heating structure and the cooling structure can be avoided, thereby improving the accuracy of temperature control. The positions of the heating structure and the cooling structure are reasonably set within the limited space at the bottom of the inner tank 2, and the integration of the preparation device 100 is improved, making the structure of the entire preparation device 100 more compact.
[0044] In actual design, the control module can be set on the side of the temperature control space 41, that is, the side of the inner tank 2, so as to be away from the heating structure and the cooling structure to prevent the control module from being affected by high or low temperature and affecting its operation.
[0045] In some embodiments, a stirring member 5 is provided in the inner pot 2, and the stirring member 5 is rotatably provided at the bottom of the inner pot 2, at least a portion of the stirring member 5 is distributed opposite to the heating structure in the up and down direction, and at least another portion of the stirring member 5 is distributed opposite to the cooling structure in the up and down direction.
[0046] That is to say, the stirring member 5 can be rotated at the bottom of the inner container 2 to stir the agarose gel solution, thereby increasing the contact area between the solution and the heating structure and the cooling structure at the bottom of the inner container 2, so that the heat generated by the heating structure and the cooling structure at the bottom of the inner container 2 are more quickly transferred to the entire agarose gel solution, thereby improving the heating and cooling efficiency. In this way, the agarose gel solution can be prepared quickly and the preparation speed can be increased, and the concentration of the agarose gel solution and the internal and external temperatures can be made more uniform, thereby reducing local overheating or overcooling and improving the preparation quality of the agarose gel.
[0047] like Figure 2 As shown, a stirring member 5 is provided at the bottom of the inner tank 2, a part of the stirring member 5 is located above the heating structure and opposite to the heating structure, and the other part of the stirring member 5 is located above the refrigeration structure and opposite to the refrigeration structure. Such arrangement enables the stirring member 5 to contact the upper part of the heating structure and the refrigeration structure at the same time, and the stirring member 5 is closer to the heating structure and the refrigeration structure, which is conducive to temperature conduction. Even the heat generated by the heating structure can be effectively transferred to the solution through the stirring member 5, and the cold generated by the refrigeration structure can be effectively transferred to the solution through the stirring member 5, thereby achieving more precise temperature control.
[0048] Therefore, by arranging the stirring element 5 in the inner container 2 and distributing it opposite to the heating structure and the cooling structure, the uniformity of the solution and the temperature conduction efficiency are improved, the accuracy and stability of the temperature control are enhanced, and it is conducive to the preparation of agarose gel with higher efficiency.
[0049] In some embodiments, the agarose gel preparation device 100 further includes a driving member 6 , which is located in the temperature-controlled space 41 . The driving member 6 is connected to the stirring member 5 and is used to drive the stirring member 5 to rotate.
[0050] Specifically, the driving member 6 is used to provide stable power to the stirring member 5 to drive the stirring member 5 to rotate. The driving member 6 is installed in the temperature-controlled space 41 and can be connected to the stirring member 5 through a transmission structure to achieve power transmission from the driving member 6 to the stirring member 5, so that the stirring member 5 can rotate in the inner container 2 according to a preset speed and direction. In particular, since the stirring member 5 extends into the inner container 2, the connection between the driving member 6 and the stirring member 5 can be sealed to prevent the solution from leaking into the temperature-controlled space 41.
[0051] In an actual design, the stirring member 5 can be configured as a stirring rod, etc., and the driving member 6 can be configured as a drive motor, a drive electric motor, etc. The rotation speed of the stirring member 5 can be adjusted by adjusting the rotation speed of the driving member 6, thereby facilitating flexible adjustment of the stirring effect according to the state and temperature of the agarose solution during the preparation process. The stirring member 5 can be configured to be detachably connected to the driving member 6. This allows for easy removal of the stirring member 5 for cleaning, maintenance, etc., which is flexible and convenient. The stirring member 5 can be integrated with the driving member 6 to improve connection reliability and facilitate manufacturing.
[0052] Therefore, by setting the driving member 6 connected to the stirring member 5, the automation and accuracy of the preparation device 100 are further improved. The stable power provided by the driving member 6 enables the stirring member 5 to rotate in the inner tank 2 according to the preset speed and direction, thereby effectively mixing the various components in the agarose solution evenly and improving the concentration uniformity.
[0053] In some embodiments, the driving member 6 is located between the cooling structure and the heating structure.
[0054] In this way, the bottom space of the inner tank 2 can be fully utilized, making the layout of the entire temperature control space 41 more compact, which is conducive to reducing the overall volume of the preparation device 100, realizing the miniaturization and lightweight of the preparation device 100, and reducing the direct interference of the driving member 6 on the cooling process and the heating process. It is located at the bottom of the inner tank 2 and can be better connected with the stirring member 5 to ensure the smooth transmission of power.
[0055] In some embodiments, a heat-insulating layer 23 is provided on the outside of the inner liner 2 , and the heating structure and the cooling structure conduct heat to the inner liner 2 through the heat-insulating layer 23 .
[0056] Specifically, if Figure 2 As shown, the outer side of the inner liner 2 is wrapped with an insulation layer 23. The heat generated by the heating structure and the cooling energy generated by the cooling structure can be effectively transferred to the wall of the inner liner 2 through the insulation layer 23, preventing heat and cooling energy loss, thereby improving the heating and cooling efficiency of the inner liner 2. The insulation layer 23 can reduce the loss of heat and cooling energy from the inner liner 2, which means that energy consumption is reduced. While achieving the same temperature control effect, the operating cost of the preparation device 100 can be reduced, resulting in greater energy savings.
[0057] In some embodiments, the refrigeration structure includes a semiconductor refrigeration plate 321 , and the cold end of the semiconductor refrigeration plate 321 is used to cool the inner container 2 .
[0058] Semiconductor cooling element 321, also known as a thermoelectric cooling element, is a heat pump with no moving parts, virtually silent and vibration-free operation. Its small size and light weight make it easy to integrate into compact devices. It eliminates the need for refrigerants and instead utilizes the Peltier effect of semiconductor materials. When direct current passes through a galvanic couple composed of two different semiconductor materials connected in series, heat is absorbed and released at each end of the galvanic couple, achieving cooling with high reliability.
[0059] Specifically, if Figure 2 As shown, a semiconductor refrigeration plate 321 is provided in the refrigeration structure and is located at the bottom of the inner liner 2. It is small in size and occupies little space, thereby further improving the integration and compactness of the preparation device 100. The cold end of the semiconductor refrigeration plate 321 can be in contact with and connected to the thermal insulation layer 23, which is beneficial for absorbing heat at the cold end of the semiconductor refrigeration plate 321 to cool the inner liner 2 when current passes through, thereby achieving cooling of the inner liner 2.
[0060] Among them, it should be noted that the current size and polarity of the semiconductor refrigeration plate 321 can be adjusted to accurately control its cooling capacity, thereby achieving precise temperature control of the inner tank 2. Since the semiconductor refrigeration plate 321 uses electron transmission rather than heat conduction, its cooling response speed is very fast, which can achieve rapid cooling of the inner tank 2 and improve temperature control efficiency.
[0061] In other embodiments, cold is transferred between the refrigeration structure and the inner liner 2 via a cooling fin 7, that is, the cooling fin 7 is arranged between the refrigeration structure and the inner liner 2 to transfer the cold generated by the refrigeration structure to the inner liner 2, thereby cooling the inner liner 2.
[0062] Specifically, the cooling fins 7 serve as a heat conduction medium with good thermal conductivity, and can quickly transfer the cold energy generated by the refrigeration structure to the inner liner 2. The cooling fins 7 can also evenly distribute the cold energy on the inner liner 2 to avoid local overcooling or overheating of the inner liner 2, thereby improving the refrigeration effect and the temperature uniformity of the inner liner 2.
[0063] like Figure 2As shown, the refrigeration structure includes a semiconductor refrigeration plate 321, which is arranged in the temperature control space 41, between the top of the semiconductor refrigeration plate 321 and the bottom of the inner tank 2, one end of which is in contact with the insulation layer 23, and the other end of which is in contact with the cold end of the semiconductor refrigeration plate 321, so that the cold energy generated by the semiconductor refrigeration plate 321 is quickly and evenly transferred to the insulation layer 23 through the cooling plate 7, and then transferred to the inner tank 2, thereby achieving rapid cooling of the inner tank 2.
[0064] In some embodiments, the heating structure includes a PTC heating sheet 311 .
[0065] Specifically, the core material of the PTC heating sheet 311 is semiconductor ceramic, which has a simple structure and is easy to install and maintain. It has the advantages of rapid heating, fast temperature compensation, high power density, and high heating temperature. When the temperature rises, the resistance value will increase significantly, so that when the set temperature is reached, the output of current and power can be automatically limited, and the heat output can be automatically reduced to achieve the effect of self-regulating temperature. In other words, the PTC heating sheet 311 can maintain a constant temperature during the heating process without the need for an external temperature control system, thereby ensuring more uniform heating.
[0066] like Figure 2 As shown, the heating structure includes a PTC heating element 311, which is installed in the temperature-controlled space 41 and horizontally separated from the semiconductor cooling element 321 to prevent mutual influence between the two. The PTC heating element 311 has a simple structure and occupies a small space, thereby further improving the integration and compactness of the preparation device 100. In practice, when current flows through the PTC heating element 311, it can quickly heat up and maintain a high temperature to heat the inner liner 2, thereby achieving rapid temperature increase of the inner liner 2.
[0067] Therefore, by using the semiconductor cooling sheet 321 and the PTC heating sheet 311 to cool down and heat up the inner tank 2, the heating and cooling conversion is made more flexible and reliable, the energy consumption is reduced, and the temperature control efficiency and accuracy are improved.
[0068] In some embodiments, the refrigeration structure is provided with a heat sink 8 on the side away from the inner tank 2. The heat sink 8 is used to dissipate heat from the refrigeration structure, reduce the temperature of the refrigeration structure, ensure that it operates within the optimal temperature range, prevent the refrigeration structure from overheating and damage, thereby improving the refrigeration efficiency.
[0069] Specifically, if Figure 2 As shown, a heat sink 8 is provided on the side of the refrigeration structure, i.e., the semiconductor refrigeration plate 321 away from the inner tank 2, i.e., the lower end of the semiconductor refrigeration plate 321. The heat sink 8 is used to dissipate heat from the hot end of the semiconductor refrigeration plate 321, so as to quickly transfer the heat generated by the hot end to the external environment, thereby effectively reducing the temperature of the semiconductor refrigeration plate 321, preventing it from overheating and damage, and improving the refrigeration efficiency.
[0070] In other embodiments, the heating structure is provided with a heat sink 8 on the side away from the inner tank 2. The heat sink 8 is used to dissipate heat from the heating structure, reduce the temperature of the refrigeration structure, ensure that it operates within the optimal temperature range, prevent the heating structure from overheating and damage, and thus improve the heating efficiency.
[0071] Specifically, if Figure 2 As shown, a heat sink 8 is provided on the side of the heating structure, i.e., the PTC heating sheet 311 away from the inner tank 2, i.e., the lower end of the PTC heating sheet 311. The heat sink 8 is used to dissipate heat from the PTC heating sheet 311, so as to quickly transfer the heat generated by the PTC heating sheet 311 to the external environment, thereby effectively reducing the temperature of the PTC heating sheet 311, preventing it from overheating and damage, and improving the heating efficiency.
[0072] In some embodiments, the liner 2 includes a liner body 21 and a liner sealing cover 22 . The liner body 21 forms a preparation space 211 open upward. The liner sealing cover 22 is detachably connected to the upper end of the liner body 21 to close the preparation space 211 .
[0073] Specifically, if Figure 2 As shown, the liner 2 includes a liner body 21 and a liner sealing cover 22. The liner body 21 can be constructed into a cylindrical shape. The interior of the liner body 21 is hollow and open upward to form an upwardly open preparation space 211. In this way, it is convenient for the operator to put agarose into the liner body 21 from above when initially using the preparation device 100 to prepare agarose gel. After placement, the preparation is started. At this time, the liner sealing cover 22 can be connected to the upper end of the liner body 21 by a threaded connection, a snap connection, etc. to close the preparation space 211, and start heating to melt the agarose to convert it into an agarose solution. The sealed preparation space 211 can reduce the volatilization of water in the agarose solution, thereby ensuring the accurate concentration of the agarose solution and reducing the pollution and erosion of the volatile components on the internal component circuits of the device, thereby extending the service life.
[0074] Among them, the shape and size of the liner sealing cover 22 should be compatible with the shape and size of the liner body 21, so as to form a tight seal around the upper end of the liner body 21 and improve the sealing effect. In actual design, the liner body 21 can be made of stainless steel. Stainless steel has good thermal conductivity, which can quickly heat up or cool down the agarose gel solution in the liner body 21, thereby improving the preparation efficiency. An internal tapping thread can be set on the edge of the outer peripheral wall of the liner sealing cover 22, and a matching external thread can be set on the edge of the inner wall peripheral edge of the liner body 21, thereby forming a threaded connection to achieve the connection and sealing between the liner body 21 and the liner sealing cover 22. Alternatively, a buckle and a slot can be set on the liner sealing cover 22 or the liner body 21 respectively, and the buckle is buckled into the slot to achieve the connection and sealing between the liner body 21 and the liner sealing cover 22.
[0075] It should be noted that the liner body 21 can be set to be detachable, that is, it is set separately from the shell 1 and can be taken out from the shell 1, so as to facilitate the cleaning and maintenance of the liner body 21 and the liner sealing cover 22. The liner body 21 can be set to be integrated, that is, it is integrated with the shell 1, which is convenient for manufacturing. The liner body 21 can be directly cleaned after use. The liner body 21 can be set to multiple compartments or a single compartment. When multiple compartments are set, each compartment can be prepared and the temperature can be controlled separately to facilitate the preparation of agarose solutions of different concentrations. When a single compartment is set, the volume is larger and more agarose gel can be prepared at one time.
[0076] The agarose gel preparation device 100 of this embodiment can be set to multiple application modes, as shown below:
[0077] (1) Application mode 1: Sol mode
[0078] The sol mode can be selected through the control module. The sol mode can be preset into three modes: standard mode, fast mode and low temperature mode. The user can select the required sol mode according to actual needs. Taking the standard mode as an example, in this mode, the control module controls the heating structure to continuously heat to increase the temperature of the inner tank 2; at the same time, the control module can control the driving member 6 to work to drive the stirring member 5 to rotate; at the same time, the temperature detection element starts to monitor the temperature of the insulation layer 23. When it is detected that the temperature of the insulation layer 23 reaches the melting point of agarose, for example 90°C, the control module can start timing.
[0079] Thus, the agarose can be heated and melted through the sol mode to convert it into an agarose solution.
[0080] (2) Application mode 2: Cooling mode
[0081] The control module can be used to select a cooling mode, which can be selected by the user according to actual needs. In this mode, the control module can control the heating structure to stop heating; at the same time, the control module can control the cooling structure to start cooling; at the same time, the control module can control the drive member 6 to operate to drive the stirring member 5 to rotate; at the same time, the temperature detection element begins to monitor the temperature of the insulation layer 23. When the temperature of the insulation layer 23 reaches the required agarose solution temperature, for example, 50°C, a specific temperature value can be set according to actual needs, at this time, the control module can start timing.
[0082] Therefore, the cooling mode can be used to cool down the melted high-temperature agarose solution.
[0083] (3) Application mode three: insulation mode
[0084] The temperature of the heat preservation layer 23 can be monitored by the temperature detection element in the heat preservation mode, and when the temperature of the heat preservation layer 23 is detected to be higher or lower than 50℃, the temperature detection element feeds back the temperature value to the control module in the form of an electrical signal, and the control module determines the actual temperature value of the heat preservation layer 23 and the set target temperature value to control the heating structure and the refrigeration structure to make corresponding adjustments. For example, when the control module receives the real-time temperature value fed back by the temperature detection element and the real-time temperature value is higher than the set target temperature value, the control module controls the refrigeration structure to continue refrigeration, and when the real-time temperature value is lowered to the set target temperature value, the control module controls the refrigeration structure to stop refrigeration, and vice versa, the control module controls the heating structure to start heating. At the same time, the control module can control the driving member 6 to drive the stirring member 5 to rotate.
[0085] Therefore, the heat preservation mode can be used to heat preserve the high-temperature agarose solution and maintain the temperature of the high-temperature agarose solution at a certain temperature, so as to avoid that the temperature is too low or too high.
[0086] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the exemplary description of the above terms does not necessarily mean the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0087] Although the embodiments of the present application have been shown and described, those skilled in the art can understand that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and purposes of the present application, and the scope of the present application is defined by the claims and their equivalents.
Claims
1. A device for preparing agarose gel, characterized in that: include: case; An inner liner is installed in the shell, and a preparation space is formed in the inner liner; A temperature control component includes a control module, a temperature detection element, a heating structure and a cooling structure. The temperature detection element, the heating structure and the cooling structure are electrically connected to the control module respectively. The temperature detection element is used to detect the temperature of the inner tank. The control module is suitable for controlling the heating structure to heat the inner tank or controlling the cooling structure to cool the inner tank according to the detection value of the temperature detection element.
2. The agarose gel preparation device according to claim 1, characterized in that: A temperature control space is formed between the inner container and the shell, and the temperature control component is installed in the temperature control space; The heating structure and the cooling structure are both located at the bottom of the inner container and are spaced apart in the horizontal direction.
3. The agarose gel preparation device according to claim 2, characterized in that: A stirring member is provided in the inner pot, and the stirring member is rotatably provided at the bottom of the inner pot. At least a portion of the stirring member is distributed opposite to the heating structure in the up and down direction, and at least another portion of the stirring member is distributed opposite to the cooling structure in the up and down direction.
4. The agarose gel preparation device according to claim 3, characterized in that: It also includes a driving member, which is located in the temperature-controlled space and is connected to the stirring member and is used to drive the stirring member to rotate.
5. The agarose gel preparation device according to claim 4, characterized in that: The driving component is located between the refrigeration structure and the heating structure.
6. The agarose gel preparation device according to claim 1, characterized in that: A heat preservation layer is provided on the outer side of the inner container, and the heating structure and the cooling structure conduct heat to the inner container through the heat preservation layer respectively.
7. The agarose gel preparation device according to claim 1, characterized in that: The refrigeration structure includes a semiconductor refrigeration plate, and the cold end of the semiconductor refrigeration plate is used to cool the inner container; And / or, cold energy is transferred between the refrigeration structure and the inner container via cooling fins.
8. The agarose gel preparation device according to claim 1, characterized in that: The heating structure includes a PTC heating sheet.
9. The agarose gel preparation device according to claim 1, characterized in that: The cooling structure is provided with a heat sink on a side away from the inner container, and / or the heating structure is provided with a heat sink on a side away from the inner container.
10. The agarose gel preparation device according to claim 1, characterized in that: The liner includes a liner body and a liner sealing cover. The liner body forms the preparation space open upward. The liner sealing cover is detachably connected to the upper end of the liner body to close the preparation space.