Bioreactor for cell culture
By designing a mixing chamber, guide troughs, reaction chamber and leakage chamber in the bioreactor, and using a motor-driven stirring device and a staggered baffle filter structure, the problems of uneven nutrient solution distribution and bacterial growth are solved, ensuring the cell culture effect.
Patent Information
- Application Number
- CN202422696561.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-05
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-11-05
AI Technical Summary
The uneven distribution of nutrient solution in existing bioreactors and the incomplete absorption of nutrient solution breed bacteria, affecting the cell culture effect.
A bioreactor consisting of a mixing chamber, a guide trough, a reaction chamber, and a leakage chamber was designed. A waterproof motor was used to drive a stirring rod and stirring blades to mix the nutrient solution. Staggered baffles and filters were used to prevent unmixed nutrient solution from entering the reaction chamber. An electric rotating disk and a leakage chamber were used to collect excess nutrient solution and discharge it regularly.
The nutrient solution is evenly distributed and discharged in time, which keeps the reactor clean, avoids bacterial growth, and promotes normal cell culture.
Smart Images
Figure CN223422664U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a bioreactor for cell culture, belonging to the technical field of cell culture. Background Art
[0002] A bioreactor is a device that uses living cells or enzymes as biocatalysts to provide a suitable environment for cell proliferation or biochemical reactions. It is a key device in the biological reaction process and can effectively increase the culture density of cells per unit volume. During cell culture, a sterile environment needs to be maintained. However, during the cell culture process, there will be some uncertainties that may cause some cells to fail to grow normally. The bacteria produced inside the reactor will also have an adverse effect on the normal growth of cells.
[0003] The nutrient solution used in existing bioreactors is mostly a mixture of multiple nutrients. When spraying and watering, the nutrient solution will be unevenly distributed, which will affect the cell culture effect. In addition, if the incompletely absorbed nutrient solution cannot be discharged in time, it will also cause bacteria to grow and contaminate the cells being cultured in the reactor. All of these will affect the normal cell culture. Therefore, a cell culture bioreactor is proposed to solve the problems of nutrient solution mixing and uneven spraying. Excess nutrient solution can be discharged in time, keeping the reactor clean and avoiding bacterial growth, which is conducive to normal cell culture. Summary of the Invention
[0004] In response to the problems in the prior art, the utility model provides a bioreactor for cell culture, which can solve the problems of nutrient solution mixing and uneven spraying, and the excess nutrient solution can be discharged in time to keep the reactor clean, which is beneficial to cell culture.
[0005] The technical solution adopted by the utility model to solve the technical problem is a bioreactor for cell culture, comprising a mixing chamber, a guide groove, a reaction chamber and a leakage chamber, wherein the top of the mixing chamber is respectively provided with a dosing port and an air injection hole, a guide groove is provided on one side of the mixing chamber, sealing strips are provided on all sides of the guide groove, a toggle rod is passed through the sealing strip, a reaction chamber is provided at the bottom of the mixing chamber, an exhaust valve is provided at the bottom of one side of the reaction chamber, a leakage chamber is installed at the bottom of the reaction chamber through a reserved opening and bolts, and support columns are provided on the periphery of the bottom of the leakage chamber;
[0006] A filter screen is provided at the bottom of the mixing chamber, stirring blades are provided between the mixing chamber and the filter screen, and a cell culture dish is provided between the bottom of the reaction chamber corresponding to the filter screen and the top of the leakage chamber.
[0007] Specifically, a waterproof motor is provided at the top center of the filter screen, a stirring rod is connected between the mixing bin and the filter screen through a rotating shaft, the outer periphery of the stirring rod is connected to the stirring blade through bolts, and the bottom of the stirring rod is connected to the driving end of the waterproof motor through a rotating shaft.
[0008] Specifically, a rotation damper is provided at the bottom of the filter, a baffle is provided at the bottom of the rotation damper, and the baffle is in close contact with the surface of the filter, the top surface of the baffle is provided with mesh holes, the mesh holes on the surface of the baffle and the filter are staggered, one side of the baffle is connected to the toggle rod by a bolt, and one end of the toggle rod passes through the guide groove and the sealing strip.
[0009] Specifically, the baffle is located in the top reserved opening of the reaction chamber.
[0010] Specifically, an electric rotating disk is provided at the center of the bottom of the reaction chamber, the top of the electric rotating disk is connected to the cell culture plate via bolts, and mesh holes are provided at the bottom of the reaction chamber.
[0011] Specifically, a drain valve is installed at a position between the support columns corresponding to the bottom of the leakage bin through a reserved opening and bolts.
[0012] Beneficial effects of the utility model:
[0013] (1) The utility model discloses a bioreactor for cell culture, which is provided with a stirring rod and a stirring blade. The stirring rod is driven by a waterproof motor to rotate the stirring blade to fully and evenly stir the various nutrient solutions. Then, the mesh holes on the surface of the baffle are vertically connected to the filter screen by rotating the toggle lever. At the same time, the electric rotating disk drives the cell culture plate to rotate, so that the cells in the cell culture plate can directly receive the irrigation of the nutrient solution.
[0014] (2) The utility model discloses a bioreactor for cell culture, wherein the mesh holes of the baffle and the filter are interlaced to prevent the nutrient solution from leaking into the reaction chamber. The excess nutrient solution flows through the mesh holes at the bottom of the reaction chamber and falls into the leakage chamber for centralized collection. The drain valve is opened regularly to discharge the excess nutrient solution to prevent bacteria from growing inside the reactor and affecting the cell culture. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is an overall schematic diagram of the utility model;
[0016] Figure 2 This is a schematic diagram of a mixing bin of the present utility model;
[0017] Figure 3 This is a schematic diagram of the reaction chamber of the present utility model;
[0018] In the figure: 1. Mixing chamber; 2. Dosing port; 3. Air injection hole; 4. Guide groove; 5. Sealing strip; 6. Filter; 7. Waterproof motor; 8. Stirring stick; 9. Stirring blade; 10. Rotation damper; 11. Baffle; 12. Toggle lever; 13. Reaction chamber; 14. Exhaust valve; 15. Electric rotating disk; 16. Cell culture plate; 17. Leakage chamber; 18. Drain valve; 19. Support column. DETAILED DESCRIPTION
[0019] In order to make the present invention more clearly understood, preferred embodiments are described in detail with reference to the accompanying drawings as follows:
[0020] like Figure 1-3 As shown, a cell culture bioreactor according to the present invention comprises a mixing chamber 1, a guide groove 4, a reaction chamber 13 and a leakage chamber 17. The top of the mixing chamber 1 is respectively provided with a dosing port 2 and an air injection hole 3. A guide groove 4 is provided on one side of the mixing chamber 1. A sealing strip 5 is provided around the guide groove 4. A toggle rod 12 runs through the sealing strip 5. A reaction chamber 13 is provided at the bottom of the mixing chamber 1. An exhaust valve 14 is provided at the bottom of one side of the reaction chamber 13. A leakage chamber 17 is installed at the bottom of the reaction chamber 13 through a reserved opening and bolts. Support columns 19 are provided on the periphery of the bottom of the leakage chamber 17.
[0021] A filter screen 6 is provided at the bottom of the mixing chamber 1 , and stirring blades 9 are provided between the mixing chamber 1 and the filter screen 6 . A cell culture dish 16 is provided between the bottom of the reaction chamber 13 corresponding to the filter screen 6 and the top of the leakage chamber 17 .
[0022] When in use, multiple nutrient solutions are added into the mixing chamber 1 through the dosing port 2;
[0023] To prevent unmixed nutrient solution from leaking into the reaction chamber 13, the mesh of the baffle 11 and the filter 6 are interlaced;
[0024] The waterproof motor 7 drives the stirring rod 8 to drive the stirring blade 9 to rotate, thereby achieving sufficient stirring and uniform mixing of the multiple nutrient solutions put into the mixing bin 1;
[0025] Then, the toggle lever 12 is moved along the guide groove 4 to rotate the baffle 11, so that the mesh holes on the surface of the baffle 11 are vertically connected to the filter 6, allowing the nutrient solution to fall onto the cell culture plate 16 in the reaction chamber 13, and the exhaust valve 14 is opened to discharge the gas in the reaction chamber 13;
[0026] The electric rotating disk 15 then drives the cell culture plate 16 to rotate, so that the cells in the cell culture plate 16 can directly receive the nutrient solution for irrigation, while the excess nutrient solution falls into the leakage chamber 17 through the mesh at the bottom of the reaction chamber 13 and is collected centrally.
[0027] The operator then regularly opens the drain valve 18 to discharge excess nutrient solution to prevent bacteria from growing inside the reactor and affecting cell culture.
[0028] In order to fully mix the nutrient solution, exemplary, as Figure 1 、 Figure 2 As shown, the utility model also includes a waterproof motor 7 provided at the top center position of the filter 6, a stirring rod 8 is connected between the mixing chamber 1 and the filter 6 through a rotating shaft, the outer periphery of the stirring rod 8 is connected to the stirring blade 9 through bolts, and the bottom of the stirring rod 8 is connected to the driving end of the waterproof motor 7 through a rotating shaft.
[0029] When in use, the waterproof motor 7 drives the stirring rod 8 to drive the stirring blade 9 to rotate, thereby achieving sufficient stirring and uniform mixing of the various nutrient solutions put into the mixing bin 1.
[0030] For example, Figure 1 、 Figure 2 As shown, the utility model also includes that a rotation damper 10 is provided at the bottom of the filter 6, a baffle 11 is provided at the bottom of the rotation damper 10, and the baffle 11 is in close contact with the surface of the filter 6, and the top surface of the baffle 11 is provided with mesh holes, and the mesh holes on the surface of the baffle 11 are staggered with the filter 6, one side of the baffle 11 is connected to the toggle rod 12 by a bolt, and one end of the toggle rod 12 passes through the guide groove 4 and the sealing strip 5.
[0031] For example, Figure 1 、 Figure 2 、 Figure 3 As shown, the present invention further includes that the baffle 11 is located in the top reserved opening of the reaction chamber 13 .
[0032] During use, to prevent unmixed nutrient solution from leaking into the reaction chamber 13, the mesh holes of the baffle 11 and the filter screen 6 are intertwined with each other, and then the toggle rod 12 is toggled along the guide groove 4 to drive the baffle 11 to rotate, so that the mesh holes on the surface of the baffle 11 are vertically connected to the filter screen 6, so that the nutrient solution falls onto the cell culture plate 16 in the reaction chamber 13.
[0033] For example, Figure 1 、 Figure 3 As shown, the present invention further includes an electric rotating disk 15 provided at the center of the bottom of the reaction chamber 13 , the top of the electric rotating disk 15 is connected to the cell culture plate 16 by bolts, and mesh holes are provided at the bottom of the reaction chamber 13 .
[0034] During use, the electric rotating disk 15 drives the cell culture plate 16 to rotate, so that the cell bodies of the cell culture plate 16 can be directly irrigated with nutrient solution, and the excess nutrient solution falls into the leakage chamber 17 along the mesh at the bottom of the reaction chamber 13 and is collected centrally.
[0035] For example, Figure 1 、 Figure 3 As shown, the present invention further includes that a drain valve 18 is installed at a position between corresponding support columns 19 at the bottom of the leakage bin 17 through a reserved opening and bolts.
[0036] When in use, the support column 19 supports the mixing chamber 1, the reaction chamber 13 and the leakage chamber 17 away from the ground, and then the drain valve 18 is opened regularly to discharge excess nutrient solution to prevent bacteria from growing inside the reactor and affecting cell culture.
[0037] When the utility model is in use, the toggle lever 12 is rotated so that the mesh of the baffle 11 and the filter 6 are interlaced with each other, and then a variety of nutrient solutions are added into the mixing chamber 1 through the dosing port 2 to prevent the nutrient solution from leaking into the reaction chamber 13;
[0038] The waterproof motor 7 drives the stirring rod 8 to drive the stirring blade 9 to rotate, thereby achieving sufficient and uniform mixing of the multiple nutrient solutions put into the mixing bin 1;
[0039] Then, the toggle lever 12 is moved along the guide groove 4 to rotate the baffle 11, so that the mesh holes on the surface of the baffle 11 are vertically connected to the filter 6, allowing the nutrient solution to fall onto the cell culture plate 16 in the reaction chamber 13. At the same time, the exhaust valve 14 can be opened to discharge the gas in the reaction chamber 13.
[0040] The electric rotating disk 15 is turned on to drive the cell culture plate 16 to rotate, so that the cells in the cell culture plate 16 can directly receive the nutrient solution irrigation, and the excess nutrient solution falls into the leakage chamber 17 through the mesh at the bottom of the reaction chamber 13 and is collected;
[0041] The operator then regularly opens the drain valve 18 to discharge excess nutrient solution to prevent bacteria from growing inside the reactor and affecting cell culture.
[0042] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form or substance. It should be pointed out that ordinary technicians in this technical field can make several improvements and supplements without departing from the present invention, and these improvements and supplements should also be considered as the scope of protection of the present invention. Any technician familiar with this profession can make some changes, modifications and equivalent changes made by using the technical content disclosed above without departing from the spirit and scope of the present invention, which are all equivalent embodiments of the present invention; at the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of the technical solution of the present invention.
Claims
1. A bioreactor for cell culture, characterized in that: The invention comprises a mixing chamber (1), a guide groove (4), a reaction chamber (13) and a leakage chamber (17), wherein the top of the mixing chamber (1) is provided with a dosing port (2) and an air injection hole (3), a guide groove (4) is provided on one side of the mixing chamber (1), sealing strips (5) are provided on all sides of the guide groove (4), and a toggle rod (12) is passed through the sealing strip (5), a reaction chamber (13) is provided at the bottom of the mixing chamber (1), an exhaust valve (14) is provided at the bottom of one side of the reaction chamber (13), a leakage chamber (17) is installed at the bottom of the reaction chamber (13) through a reserved opening and bolts, and support columns (19) are provided on the periphery of the bottom of the leakage chamber (17); A filter screen (6) is provided at the bottom of the mixing chamber (1), stirring blades (9) are provided between the mixing chamber (1) and the filter screen (6), and a cell culture dish (16) is provided between the bottom of the corresponding filter screen (6) and the top of the leakage chamber (17) in the reaction chamber (13).
2. A cell culture bioreactor according to claim 1, characterized in that: A waterproof motor (7) is provided at the center of the top of the filter (6); a stirring rod (8) is connected between the mixing chamber (1) and the filter (6) via a rotating shaft; the outer periphery of the stirring rod (8) is connected to the stirring blade (9) via a bolt; and the bottom of the stirring rod (8) is connected to the driving end of the waterproof motor (7) via a rotating shaft.
3. A cell culture bioreactor according to claim 1, characterized in that: The bottom of the filter (6) is provided with a rotation damper (10), the bottom of the rotation damper (10) is provided with a baffle (11), and the baffle (11) and the surface of the filter (6) are in close contact with each other, the top surface of the baffle (11) is provided with mesh holes, the mesh holes on the surface of the baffle (11) and the filter (6) are staggered with each other, one side of the baffle (11) is connected to the toggle rod (12) by a bolt, and one end of the toggle rod (12) passes through the guide groove (4) and the sealing strip (5).
4. A cell culture bioreactor according to claim 3, characterized in that: The baffle (11) is located in the top reserved opening of the reaction chamber (13).
5. The cell culture bioreactor according to claim 1, characterized in that: An electric rotating disk (15) is provided at the center of the bottom of the reaction chamber (13). The top of the electric rotating disk (15) is connected to the cell culture plate (16) via bolts. Mesh holes are provided at the bottom of the reaction chamber (13).
6. A bioreactor for cell culture according to claim 1, characterized in that: The bottom of the leakage bin (17) corresponds to the position between the support columns (19) and a drain valve (18) is installed through a reserved opening and bolts.