Nucleic acid collection tube and droplet type nucleic acid detection device

Abstract

A nucleic acid collecting tube comprises a test tube and a tube cover, the test tube and the tube cover jointly form a sealed tube cavity, a protrusion is arranged on the top wall of the tube cover and extends towards the bottom wall of the test tube along the axis of the test tube, the tube cover is provided with a protrusion, a cotton swab is inserted into the test tube, the protrusion is pressed on the cotton swab to prevent the cotton swab from arching in an arc shape, and the probability that the cotton swab is broken at a time is increased. The collection time is shortened, and the nucleic acid collection efficiency is improved. The upper end of the ring wall of the test tube is clamped between the tube cover and the bulge, so that the covering position of the tube cover on the test tube can be stabilized, the tube cover is prevented from being released from the test tube when the cotton swab is broken, and the probability of one-time breaking of the cotton swab is influenced.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid detection technical field, especially nucleic acid collection tube and microdrop formula nucleic acid detection device. BACKGROUND

[0002] As the first line of defense for external input, one important work content is how to improve nucleic acid detection capability to meet the detection requirements of entry personnel and animals (such as sheep).

[0003] In the prior art, after collecting nucleic acid samples, the cap is unscrewed, the cotton swab is inserted into the test tube, the head of the cotton swab is pressed against one side of the test tube wall, the middle of the cotton swab is pressed against the other side of the test tube wall, the root of the cotton swab is pressed down, the head of the cotton swab is broken in the test tube, the cap is screwed on, and the sample is sent for detection.

[0004] As shown in Figure 1 When the cotton swab is bent, the cotton swab will form an arc arch, and the cotton swab is mostly bent repeatedly, which takes a long time to collect and has low nucleic acid collection efficiency. SUMMARY

[0005] Therefore, in view of the above problems, it is necessary to provide a nucleic acid collection tube.

[0006] It is also necessary to provide a microdrop nucleic acid detection device.

[0007] A nucleic acid collection tube comprises a test tube and a cap, the test tube and the cap jointly form a sealed tube cavity, the top wall of the cap is provided with a protrusion, and the protrusion extends along the axis of the test tube to the bottom wall of the test tube.

[0008] Preferably, the test tube is columnar.

[0009] Preferably, the protrusion is formed by recessing the top wall of the cap in the tube cavity.

[0010] Preferably, the inner wall of the ring wall of the cap is threadedly connected with the outer wall of the test tube.

[0011] Preferably, the ring wall of the cap and the ring wall of the protrusion are located on the same side of the top wall of the cap.

[0012] Preferably, the ring wall of the cap and the ring wall of the protrusion are coaxial.

[0013] Preferably, the protrusion is columnar.

[0014] A microdrop nucleic acid detection device comprises a nucleic acid collection tube.

[0015] Preferably, the microdrop nucleic acid detection device further comprises a microdrop generator.

[0016] Preferably, the microdrop nucleic acid detection device further comprises a microdrop analyzer.

[0017] Beneficial effects: the tube cover is provided with a protrusion, the cotton swab is inserted into the test tube, the protrusion is pressed on the cotton swab, the cotton swab is prevented from being arched, the probability of one-time breaking of the cotton swab is improved, the collection time is reduced, and the nucleic acid collection efficiency is improved. The upper end of the ring wall of the test tube is clamped between the tube cover and the protrusion, the tube cover is stably capped on the test tube, when the cotton swab is broken, the tube cover is prevented from being buckled off the test tube, and the probability of one-time breaking of the cotton swab is affected. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a schematic diagram of the cotton swab being broken in the test tube in the prior art.

[0019] Figure 2 It is a schematic diagram of the cotton swab being broken in the test tube in the prior art.

[0020] Figure 3 It is an isometric view of the nucleic acid collection tube with the tube cover in an open state.

[0021] Figure 4 It is an isometric view of the nucleic acid collection tube with the tube cover in a closed state.

[0022] Figure 5 It is an isometric view of the tube cover provided with an end cover.

[0023] In the drawing: collection tube 10, test tube 11, tube cover 12, protrusion 13, groove 14, through hole 15, end cover 16, cotton swab 20. DETAILED DESCRIPTION

[0024] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings described in the following are some embodiments of the present application, and other drawings can also be obtained according to these drawings without creative labor for those skilled in the art.

[0025] Referring to Figures 2 to 4 The nucleic acid collection tube 10 provided by the embodiments of the present application comprises a test tube 11 and a tube cover 12, the test tube 11 and the tube cover 12 jointly form a sealed tube cavity, a top wall of the tube cover 12 is provided with a protrusion 13, and the protrusion 13 extends to a bottom wall of the test tube 11 along an axis of the test tube 11.

[0026] Exemplarily, the height of the protrusion 13 can be slightly higher or lower than the depth of the tube cover 12.

[0027] Beneficial effect: the tube cover 12 is provided with the protrusion 13, the cotton swab 20 is inserted into the test tube 11, the protrusion 13 is pressed on the cotton swab 20, the cotton swab 20 is prevented from being arched, the probability of the cotton swab 20 being broken once is improved, the time of collection is reduced, and the efficiency of nucleic acid collection is improved. The upper end of the ring wall of the test tube 11 is clamped between the tube cover 12 and the protrusion 13, the tube cover 12 can be stably covered on the test tube 11, and when the cotton swab 20 is broken, the tube cover 12 is prevented from being buckled off the test tube 11, thereby affecting the probability of the cotton swab 20 being broken once.

[0028] In one embodiment, the lumen is spiral-shaped. After the cotton swab 20 enters the spiral-shaped lumen and is broken, the adherend on the cotton swab 20 is not easy to splash out of the lumen.

[0029] Referring to Figures 2 to 4 , further, the test tube 11 is columnar. Illustratively, the upper end of the test tube 11 is open, the lower end of the test tube 11 is closed, and the tube cover 12 is arranged at the top opening of the test tube 11.

[0030] Referring to Figures 2 to 4 , further, the protrusion 13 is formed by the top wall of the tube cover 12 being recessed in the lumen.

[0031] Referring to Figures 2 to 4 , further, the inner wall of the ring wall of the tube cover 12 is threadedly connected with the outer wall of the test tube 11.

[0032] Referring to Figures 2 to 4 , further, the ring wall of the tube cover 12 and the ring wall of the protrusion 13 are located on the same side of the top wall of the tube cover 12.

[0033] Referring to Figures 2 to 4 , further, the ring wall of the tube cover 12 and the ring wall of the protrusion 13 are coaxial.

[0034] Referring to Figures 2 to 4 , further, the protrusion 13 is columnar. Illustratively, the upper end of the ring wall of the test tube 11 is clamped between the ring wall of the tube cover 12 and the ring wall of the protrusion 13.

[0035] Illustratively, referring to Figure 5 , the top wall of the tube cover 12 is recessed in the lumen to form a columnar groove 14, a through hole 15 is arranged on the bottom wall of the groove 14, an end cover 16 is arranged on the through hole 15, a first friction layer is arranged on the outer ring wall of the end cover 16, a second friction layer is arranged on the inner ring wall of the groove 14, the first friction layer and the second friction layer are in contact, a certain pulling force is required to separate the end cover 16 from the through hole 15, the end cover 16 is convenient to open and close, and the end cover 16 and the tube cover 12 do not need to be designed to be connected, for example, the end cover 16 and the tube cover 12 are hingedly connected. The cotton swab 20 is loaded into the test tube 11 through the through hole 15, and the tube cover 12 does not need to be opened, and compared with the structure that the test tube 11 is made into a closed tube and a through hole 15 is arranged at one end, the cost is lower. A pull ring can be arranged on the end cover 16, so that the end cover 16 is conveniently pulled out of the groove 14.

[0036] Referring to Figures 2 to 4 The micro-droplet nucleic acid detection device provided in the embodiment of the utility model comprises a nucleic acid collection tube 10.

[0037] In one embodiment, the bottom of the test tube 11 is provided with a suction cup, and the micro-droplet nucleic acid detection device further comprises a storage disc, which is a rectangular plate, and the upper surface of the storage disc is a smooth plane.

[0038] Further, the micro-droplet nucleic acid detection device further comprises a micro-droplet generator.

[0039] Further, the micro-droplet nucleic acid detection device further comprises a micro-droplet analyzer.

[0040] The nucleic acid collection tube 10 is used for collecting a nucleic acid sample; the storage disc is used for placing a plurality of nucleic acid collection tubes 10 after collection; the transfer box is used for loading the nucleic acid collection tubes 10 full of the storage disc and sending the nucleic acid collection tubes 10 to a designated place to extract a reaction liquid; the micro-droplet generator is used for generating 20,000 nanoliter-sized droplets from the reaction liquid, each droplet containing or not containing a nucleic acid target molecule, each micro-droplet serving as an independent PCR reactor and then being subjected to PCR amplification; and the micro-droplet analyzer is used for detecting each micro-droplet one by one, a fluorescent signal being judged as “1” and no fluorescent signal being judged as “0”, and finally the concentration of the target molecule to be detected being calculated by an analysis software according to the Poisson distribution principle and the proportion of positive micro-droplets.

[0041] The micro-droplet generator is specifically a QX200 micro-droplet generator, the micro-droplet analyzer is specifically a QX200 micro-droplet analyzer, and the QX200 micro-droplet analyzer and the QX200 micro-droplet generator form a QX200 Droplet Digital PCR system.

[0042] The so-called “micro-droplet type” refers to a method of generating droplets from a reaction liquid, and the micro-droplet generator provided in the micro-droplet nucleic acid detection device can realize absolute quantification and multiplex detection, the multiplex detection can be understood as detecting a plurality of pathogens at the same time, the detection process is rapid and has high precision; and the micro-droplet generator provided in the micro-droplet nucleic acid detection device can realize digitalization and visualization of detection results.

[0043] The modules or units in the device in the embodiment of the utility model can be combined, divided and reduced according to actual needs.

[0044] The above disclosure is only the preferred embodiment of the utility model, and of course cannot limit the scope of the utility model, and those skilled in the art can understand that all or part of the processes of the above embodiments are implemented, and equivalent changes are made according to the utility model claims, which still belong to the scope covered by the utility model.

Claims

1. A nucleic acid collection tube, characterized in that: The invention comprises a test tube and a tube cover, which together form a sealed tube cavity. The top wall of the tube cover is provided with a protrusion, which extends along the axis of the test tube toward the bottom wall of the test tube. The top wall of the tube cover is recessed in the tube cavity to form a columnar groove. A through hole is provided on the bottom wall of the groove, and an end cover is provided on the through hole. A first friction layer is provided on the outer ring wall of the end cover, and a second friction layer is provided on the inner ring wall of the groove. The first friction layer and the second friction layer are in contact, and a pull ring is installed on the end cover.

2. The nucleic acid collection tube according to claim 1, wherein: The test tube is cylindrical.

3. The nucleic acid collection tube according to claim 1, wherein: The protrusion is formed by the top wall of the tube cover being recessed in the tube cavity.

4. The nucleic acid collection tube according to claim 3, wherein: The inner wall of the ring wall of the tube cover is threadedly connected to the outer wall of the test tube.

5. The nucleic acid collection tube according to claim 3, wherein: The annular wall of the tube cover and the annular wall of the protrusion are located on the same side of the top wall of the tube cover.

6. The nucleic acid collection tube according to claim 3, wherein: The annular wall of the tube cover is coaxial with the annular wall of the protrusion.

7. The nucleic acid collection tube according to claim 3, wherein: The protrusion is columnar.

8. A droplet-type nucleic acid detection device, characterized in that: Comprising the nucleic acid collection tube according to claim 1.

9. The droplet-type nucleic acid detection device according to claim 8, wherein: The droplet-type nucleic acid detection device also includes a droplet generator.

10. The droplet-type nucleic acid detection device according to claim 9, wherein: The droplet-type nucleic acid detection device also includes a droplet analyzer.

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