Specific separation device for engineered exosome

Abstract

The utility model discloses an engineering exosome specificity separating device, relates to the centrifuge field of exosome, including centrifuge and the rotating shaft that is movably arranged in centrifuge, the outside of rotating shaft is equipped with the mother rotor, the mother rotor is composed of a plurality of son rotor, the mother rotor upper end is equipped with the upper cover, and the mother rotor lower end is equipped with the lower cover. A rubber ring is fixedly arranged at the lower end of the upper cover, an arc-shaped groove is formed in the upper end of each sub-rotor, and each rubber ring is clamped to the inner side of the corresponding arc-shaped groove. According to the exosome separation device, a conventional rotor is divided into the multiple equal sub-rotors, so that when a small amount of exosomes are separated, a small amount of sub-rotors can be used, and the weight of the rotor is integrally reduced; therefore, the load of the servo motor during working is reduced, the energy consumption of the servo motor is reduced, the exosomes with various components can be adapted, and the use number of the sub-rotors is diversified.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifuge field for exosome, especially in engineering exosome specificity separation device. BACKGROUND

[0002] Exosome refers to small membrane bubble (30-150nm) including complex RNA and protein, nowadays, it specifies disc-shaped vesicle with 40-100nm in diameter, and various cells can secrete exosome under normal and pathological conditions, and it is mainly derived from multivesicular body formed by endocytic lysosome microparticle, is released into extracellular matrix after multivesicular body outer membrane and cell membrane fusion, all cultured cell types can secrete exosome, and exosome exists naturally in body fluid, including blood, saliva, urine, cerebrospinal fluid and milk, and the accurate molecular mechanism of their secretion and uptake and composition, "carrying" and corresponding function is just beginning to study, and exosome is regarded as specific secretion membrane bubble;

[0003] When exosome is separated, generally separates by centrifuge, obtains supernatant and subnatant, extracts supernatant again, and separates supernatant again to separate exosome, when centrifuge is used, generally drives a rotor to rotate by motor, a plurality of storage holes are formed on the rotor to store exosome, the motor drives rotation to carry out special-shaped separation of exosome, but the storage hole on the rotor cannot be filled generally due to the quantity of exosome to be checked when a single rotor is used, so as to achieve the maximum benefit of centrifuge, and the energy consumption of motor does not decrease with the decrease of the quantity of exosome when centrifuge is used to centrifugalize and separate several exosome, so the separation cost is higher;

[0004] Therefore, it is necessary to provide engineering exosome specificity separation device to solve the above problems. UTILITY MODEL CONTENT

[0005] The utility model aims at providing engineering exosome specificity separation device to solve the problems that the energy consumption of motor does not decrease with the decrease of the quantity of exosome when a small amount of exosome is detected, the energy consumption of motor is higher, and the separation cost is higher.

[0006] To achieve the above object, the utility model provides the following technical scheme: engineering exosome specificity separation device, including centrifuge and the shaft that is movably arranged in the centrifuge, the female rotor is installed on the outer side of the shaft, the female rotor is composed of a plurality of male rotors, the upper end of the female rotor is equipped with an upper cover, the lower end of the upper cover is fixedly provided with a rubber ring, the upper end of the male rotor is provided with an arc-shaped groove, and the rubber ring is clamped in the inner side of the arc-shaped groove.

[0007] The lower end of the female rotor is conically arranged, the outer side of the female rotor is attached with a gathering shell, the outer side of the male rotor is fixedly provided with an arc-shaped plate, all the arc-shaped plates form a circular ring, and the outer side of the circular ring is provided with an external thread.

[0008] The lower end of the converging shell is fixedly provided with an annular sleeve, and an internal thread is formed in the inner wall of the annular sleeve.

[0009] Preferably, the cross sections of the arc-shaped grooves and the rubber ring are both in the shape of a right trapezoid.

[0010] Preferably, a clamping ring is fixedly arranged outside the rotating shaft, a plurality of clamping grooves are formed in the outer side of the clamping ring, and a clamping strip is fixedly arranged at one end of the sub-rotor corresponding to the rotating shaft and clamped in the inner side of the corresponding clamping groove.

[0011] The clamping strip and the clamping groove are both arranged in the shape of an annular array, the number of the clamping strip, the clamping groove and the sub-rotor is equal, and the cross sections of the clamping strip and the clamping groove are both in the shape of a convex.

[0012] A plurality of reinforcing plates are fixedly arranged at the upper end of the clamping ring, and the reinforcing plates are also fixedly connected with the rotating shaft.

[0013] Preferably, the sub-rotor is hollow, a bottom plate is fixedly arranged outside the rotating shaft, and the lower end of the sub-rotor is in contact with the upper end of the bottom plate.

[0014] Preferably, the converging shell is in the shape of a cone, and a plurality of friction balls are fixedly arranged outside the converging shell.

[0015] Preferably, a servo motor is mounted in the centrifugal machine, the output shaft of the servo motor is fixedly connected with the end of the rotating shaft, and two storage cavities are formed in the sub-rotor.

[0016] The technical effects and advantages of the present application are as follows:

[0017] 1. The conventional rotor is divided into a plurality of equal sub-rotors, so that when a small amount of exosomes is separated, a small amount of sub-rotors can be used to reduce the weight of the rotor as a whole, thereby reducing the load of the servo motor during operation, reducing the energy consumption of the servo motor, and being able to adapt to a variety of exosomes, and the number of sub-rotors used is diversified.

[0018] 2. The converging shell is arranged, the converging shell and the mother rotor are in abutment with each other, the plurality of sub-rotors are simultaneously contracted and fixed, all the sub-rotors in the converging shell can be simultaneously extruded, the sub-rotors are fixed while ensuring that all the sub-rotors are arranged in a concentric manner, and the stability of the sub-rotor under high-speed rotation is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a structural schematic view of the exosome specific separation device.

[0020] Figure 2 It is a structural schematic view of the mother rotor.

[0021] Figure 3 This is a schematic diagram of the cross-sectional structure of the mother rotor of the utility model.

[0022] Figure 4 This is a schematic diagram of the sub-rotor matching structure of the utility model.

[0023] Figure 5 This is a schematic diagram of the structure of the gathered shell and curved plate of the utility model.

[0024] Figure 6 This is a schematic diagram of the sub-rotor structure of the utility model.

[0025] In the figure: 1. centrifuge; 2. mother rotor; 3. daughter rotor; 4. rotating shaft; 5. upper cover; 6. arc plate; 7. gathering shell; 8. annular sleeve; 9. arc groove; 10. rubber ring; 11. storage chamber; 12. bottom plate; 13. snap-in strip; 14. snap-in ring; 15. snap-in groove; 16. reinforcement plate. DETAILED DESCRIPTION

[0026] The utility model provides Figure 1 - Figure 6 The engineered exosome-specific separation device shown includes a centrifuge 1 and a rotating shaft 4 movably arranged in the centrifuge 1. A servo motor is installed inside the centrifuge 1, and the output shaft of the servo motor is fixedly connected to the end of the rotating shaft 4. The servo motor is a prior art and will not be described in detail here. The servo motor is used to drive the rotating shaft 4 to rotate, which is the main output power of the centrifuge 1. In order to separate the exosomes, the centrifuge 1 is also equipped with a cooling device to ensure that the separation temperature is 4°C when the exosomes are centrifuged for heterogeneous separation. The cooling device is a prior art and will not be described in detail here. A mother rotor 2 is installed outside the rotating shaft 4. It is an existing commonly used rotor. It is a prior art and will not be described in detail here. The mother rotor 2 is composed of multiple sub-rotors 3. During the exosome separation and inspection, the amount of detection is generally determined according to the actual usage. Sometimes the number of detections is more or less, depending on the situation.

[0027] A clamping ring 14 is fixedly provided on the outside of the rotating shaft 4, and a plurality of clamping grooves 15 are opened on the outside of the clamping ring 14. A clamping strip 13 is fixedly provided on the end of the sub-rotor 3 corresponding to the rotating shaft 4. The clamping strip 13 is clamped to the inner side of the corresponding clamping groove 15. The clamping strips 13 and the clamping grooves 15 are arranged in a ring array. The number of clamping strips 13, clamping grooves 15 and sub-rotor 3 is equal. The cross-sections of the clamping strips 13 and the clamping grooves 15 are both convex. A plurality of reinforcing plates 16 are fixed on the upper end of the clamping ring 14, and the reinforcing plates 16 are also fixedly connected to the rotating shaft 4.

[0028] The sub-rotor 3 is clamped on the clamping ring 14 through the clamping strip 13, and the number of sub-rotors 3 used is determined according to the exosome component separated by the centrifugal machine 1 in a single separation. When the sub-rotor 3 is used for separation, the position of the sub-rotor 3 also needs to be controlled to ensure that the rotating shaft 4 can be restrained by the multiple sub-rotors 3 when rotating, thereby ensuring the stability of rotation. The conventional rotor is divided into multiple equal sub-rotors 3, so that a small amount of sub-rotors 3 can be used for separation when a small amount of exosomes is separated, thereby reducing the weight of the rotor as a whole, reducing the load of the servo motor when working, reducing the energy consumption of the servo motor, and being able to adapt to various components of exosomes, and the number of sub-rotors 3 used is diversified.

[0029] The sub-rotor 3 is hollow, further reducing the weight of the rotor, and the bottom plate 12 is fixed outside the rotating shaft 4. The lower end of the sub-rotor 3 contacts the upper end of the bottom plate 12, and the bottom plate 12 is used to bear the sub-rotor 3. The converging shell 7 is conical, which is convenient for fitting the outside of the mother rotor 2. The converging shell 7 is fixed outside and provided with multiple friction balls, which is convenient for the operator to rotate the converging shell 7.

[0030] Two storage cavities 11 are formed in the sub-rotor 3, which are mainly used to store exosomes such as blood or saliva. The upper end of the mother rotor 2 is provided with an upper cover 5, which is mainly used to connect the upper ends of multiple sub-rotors 3. The lower end of the upper cover 5 is fixed with a rubber ring 10. The upper end of the sub-rotor 3 is provided with an arc-shaped groove 9, and the rubber ring 10 is clamped in the inner side of the arc-shaped groove 9. The cross sections of the arc-shaped groove 9 and the rubber ring 10 are both straight-angle trapezoidal. The lower end of the mother rotor 2 is conical. The converging shell 7 is fitted outside the mother rotor 2. The outer side of the converging shell 7 is fixed with an arc-shaped plate 6. All the arc-shaped plates 6 form a circular ring. The outer side of the circular ring is provided with external threads. The lower end of the converging shell 7 is fixed with a ring-shaped sleeve 8. The inner wall of the ring-shaped sleeve 8 is provided with internal threads. The ring-shaped sleeve 8 and the circular ring are connected through threads.

[0031] After the operator connects the sub-rotor 3 and the rotating shaft 4 that need to be used, the operator rotates the converging shell 7. The converging shell 7 drives the ring-shaped sleeve 8 to rotate. The ring-shaped sleeve 8 is connected with the arc-shaped plate 6 through threads, so that the converging shell 7 and the mother rotor 2 abut each other, thereby simultaneously shrinking and fixing multiple sub-rotors 3. All the sub-rotors 3 inside the converging shell 7 can be simultaneously extruded. When the sub-rotors 3 are fixed, all the sub-rotors 3 are concentrically arranged, thereby ensuring the stability of the sub-rotor 3 under high-speed rotation.

Claims

1. An engineered exosome-specific separation device comprising a centrifuge (1) and a rotating shaft (4) movably arranged inside the centrifuge (1), characterized in that: The outer side of the rotating shaft (4) is provided with a female rotor (2), the female rotor (2) is composed of a plurality of sub-rotors (3), the upper end of the female rotor (2) is provided with an upper cover (5), the lower end of the upper cover (5) is fixedly provided with a rubber ring (10), the upper end of the sub-rotor (3) is provided with an arc-shaped groove (9), and the rubber ring (10) is clamped on the inner side of the arc-shaped groove (9). The lower end of the female rotor (2) is conical, the outer side of the female rotor (2) is provided with a converging shell (7), the outer side of the sub-rotor (3) is fixedly provided with an arc-shaped plate (6), all the arc-shaped plates (6) form a circular ring, and the outer side of the circular ring is provided with external threads. The lower end of the converging shell (7) is fixedly provided with an annular sleeve (8), the inner wall of the annular sleeve (8) is provided with internal threads, and the annular sleeve (8) and the circular ring are connected through threads.

2. The engineered exosome-specific isolation device of claim 1, wherein: The cross sections of the arc-shaped groove (9) and the rubber ring (10) are all provided in a straight angle trapezoidal shape.

3. The engineered exosome-specific isolation device of claim 1, wherein: The outer side of the rotating shaft (4) is fixedly provided with a clamping ring (14), the outer side of the clamping ring (14) is provided with a plurality of clamping grooves (15), one end of the sub-rotor (3) corresponding to the rotating shaft (4) is fixedly provided with a clamping strip (13), and the clamping strip (13) is clamped on the inner side of the corresponding clamping groove (15). The clamping strip (13) and the clamping groove (15) are both provided in a ring-shaped array, the number of the clamping strip (13), the clamping groove (15) and the sub-rotor (3) is equal, and the cross sections of the clamping strip (13) and the clamping groove (15) are both provided in a convex shape. The upper end of the clamping ring (14) is fixedly provided with a plurality of reinforcing plates (16), and the reinforcing plates (16) are also fixedly connected with the rotating shaft (4).

4. The engineered exosome-specific isolation device of claim 1, wherein: The sub-rotor (3) is provided in a hollow shape, the outer side of the rotating shaft (4) is fixedly provided with a bottom plate (12), and the lower end of the sub-rotor (3) contacts the upper end of the bottom plate (12).

5. The engineered exosome-specific isolation device of claim 1, wherein: The converging shell (7) is provided in a conical shape, and the outer side of the converging shell (7) is fixedly provided with a plurality of friction balls.

6. The engineered exosome-specific isolation device of claim 1, wherein: The centrifugal machine (1) is provided with a servo motor on the inner side, the output shaft of the servo motor is fixedly connected with the end of the rotating shaft (4), and two storage cavities (11) are formed in the sub-rotor (3).

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