Animal specimen sampling cutting device
By designing a cutting device with organic glass containers and copper comb plates suitable for various tissue types, the problems of limited applicability and complex operation of cutting devices in the existing technology are solved, and low-cost, easy-to-operate, and precise slicing effects are achieved.
Patent Information
- Application Number
- CN202421911497.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-08
- Publication Date
- 2025-10-21
- Estimated Expiration
- 2034-08-08
AI Technical Summary
Existing tissue cutting devices have limited applicability, are expensive, or are complicated to operate, making it difficult to achieve precise cutting of different tissue types and individual differences. In addition, the fixed slice intervals cannot adapt to the needs of different samples.
A cutting device was designed, which includes a plexiglass container, a detachable base plate and a copper comb plate. The container and base plate are made of transparent materials. The comb plate can be detachably inserted into the base plate. The comb tooth spacing is adjustable and suitable for a variety of tissue types. The tissue is wrapped with agarose gel to control the slice thickness and direction.
It achieves low-cost, easy-to-operate precise cutting of various tissue samples, adapts to the needs of slices of different thicknesses and positions, and improves the uniformity and visibility of slices.
Smart Images
Figure CN223461282U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to animal specimen manufacturing field, especially a kind of embedding type specimen cutting device. BACKGROUND
[0002] In scientific research, paraffin or frozen sample is made, especially in recent years, the tissue transparency technology is rising, and the tissue organs need to be sampled, and the precision of sampling and the uniformity of cutting thickness have great influence on later experiment.
[0003] The current tissue cutting device is usually suitable for limited tissue types (such as brain, cerebellum or heart), and there are few devices suitable for multiple tissue types. Even if there are such devices, they are expensive or complicated to operate, which makes researchers hesitate.
[0004] In addition, even for the same kind of tissue, due to the difference in size and development degree of the individual, it is difficult to accurately cut the sample from a specific part. The existing sample cutting mold has fixed size, fixed slice interval and wide interval, which cannot meet the needs of different samples. Accordingly, there is a need to provide a low-cost and high-precision tissue cutting device. SUMMARY
[0005] In order to overcome the shortcomings of the prior art, the present application provides a cutting device for animal specimen sampling, which is low in cost, easy to operate and can solve the slicing needs of different samples.
[0006] To achieve the above purpose, the technical scheme of the utility model is as follows:
[0007] A cutting device for animal specimen sampling, characterized in that it comprises:
[0008] A container (1) having a bottom surface (11) and a side wall (12) and made of organic glass;
[0009] A bottom support plate (2) having a bottom plate (23) and four support columns (21), the support columns (21) being erected on the four corners of the bottom plate (23) and being perpendicular to the bottom plate (23), a slot (22a) being provided along one edge a of the bottom plate (23), a slot (22b) being provided along the opposite edge b of the bottom plate (23), the slot (22a) being parallel to the slot (22b), the bottom support plate (2) being detachably placed in the container (1), and the height of the support columns (21) being higher than the side wall (12) of the container (1), the bottom plate (23) and the support columns (21) being made of organic glass; and
[0010] The comb tooth plate (3) has a crossbeam (32) and comb teeth (31) arranged at intervals on the crossbeam (32), the comb tooth plate (3) is detachably inserted into the slot (22a, 22b) through the crossbeam (32), and the height of the comb tooth (31) is not lower than the side wall (12) of the container (1), and the comb tooth plate (3) is made of copper.
[0011] Further, the thickness of the comb tooth plate (3) is 1mm.
[0012] Further, the distance between the slot (22a) and the edge a is 5mm, and the distance between the slot (22b) and the edge b is 5mm.
[0013] Further, the inner diameter of the container (1) is 50mmx50mmx30mm, the height of the support column (21) is 35mm, the height of the comb tooth (31) of the comb tooth plate (3) is 23mm, and the height of the crossbeam (32) is 5mm.
[0014] Further, the comb tooth plate (3) has 21 comb teeth (31), the width of the comb tooth (31) is 1mm, and the interval of the comb tooth (31) is 0.5mm.
[0015] Further, the comb tooth plate (3) has 16 comb teeth (31), the width of the comb tooth (31) is 1.5mm, and the interval of the comb tooth (31) is 0.5mm.
[0016] Advantages
[0017] The device can make thick sections of any size and interval thickness of tissue samples in the range of 49.0mmx36.8mmx25.0mm by using different intervals of comb teeth, and the direction of the sample can be controlled by wrapping the tissue with agarose gel, which can protect and stabilize the tissue. This method is low in cost, easy to operate, and can meet the requirements of different thickness sections.
[0018] The animal sample cutting device of the present application is suitable for tissues and organ samples of various sources, sizes and developmental stages; the comb teeth are made of copper, which can meet the requirements of different cutting positions and intervals; the arrangement of the comb teeth can ensure consistent cutting thickness and accurate sampling; at the same time, the container is made of transparent organic glass material, which has good visibility during sample operation; and the device is low in cost and easy to operate. BRIEF DESCRIPTION OF DRAWINGS
[0019] Figure 1 It is a schematic view of the container structure.
[0020] Figure 2 It is a schematic view of the bottom plate structure.
[0021] Figure 3 It is a schematic view of the copper plate structure.
[0022] Figure 4 Schematic diagram of the structure of a comb plate in one embodiment.
[0023] Figure 5 Schematic diagram of the structure of the comb plate in another embodiment.
[0024] Figure 6 Schematic diagram of the internal structure of the cutting device after the comb plate is inserted.
[0025] Figure 7 Schematic diagram of the structure after the cutting device is inserted into the comb plate.
[0026] Among them: 1. Container; 11. Bottom surface; 12. Side wall; 2. Bottom support plate; 21. Pillar; 22a, 22b, slots; 23. Bottom plate; 3. Comb plate; 31. Comb teeth; 32. Crossbeam; 33. Channel; 4. Copper plate. DETAILED DESCRIPTION
[0027] The container 1 is a box with a base but no cover, having a bottom surface 11 and side walls 12. It is in the shape of a rectangular parallelepiped. The container 1 is made of a transparent material, such as organic glass. Figure 1 As shown, in one embodiment, the inner diameter of the container 1 is 50mm×50mm×30mm, the bottom 11 and the side wall 12 are both 3mm thick, and the outer diameter is 56mm×56mm×33mm.
[0028] The bottom support plate 2 has a bottom plate 23 and four pillars 21. The bottom plate 23 is adapted to the bottom of the container 1. Figure 2 As shown, in one embodiment, the bottom plate 23 is a 49mm×49mm square and 5mm thick. Four pillars 21 perpendicular to the bottom plate 23 are erected at the four corners of the bottom plate 23. The bottom support plate 2 can be detachably placed in the container 1, and the height of the pillars 21 must be higher than the side wall 12 of the container 1 so that the bottom support plate 2 can be lifted from the container 1. The pillars 21 can be cylindrical or rectangular. In one embodiment, the pillars 21 are cylindrical with a diameter of 2mm and a height of 35mm. A slot 22a is provided along one side a of the bottom plate 23, and a slot 22b is provided along the side b opposite to the side a of the bottom plate 23. The slots 22a and 22b are as close to the side a and the side b as possible, respectively. In one embodiment, the distance between the slot 22a and the side a is 5mm, and the distance between the slot 22b and the side b is 5mm. The length and width of the slot 22 are 49mm×1.1mm, and the depth is 3mm. The bottom plate 23 and the four pillars 21 are all made of transparent materials. In one embodiment, the base plate 23 and the support 21 are made of organic glass, which facilitates observation of the state of the specimen.
[0029] Comb plate 3 Figure 3The copper sheet 4 is shown. The copper sheet is soft and easy to cut. In one embodiment, the copper sheet 4 is 1 mm thick. In one embodiment, the copper sheet 4 is rectangular with a length of 49 mm and a width of 28 mm.
[0030] The comb plate 3 has a crossbeam 32 and combs 31 spaced on the crossbeam 32. The comb plate 3 has two identical pieces which are detachably inserted into the slots 22a and 22b of the base plate 2 respectively by the crossbeam 32. In one embodiment, the crossbeam 32 is 49 mm long and 5 mm high. The part of the comb 31 connected to the crossbeam 32 is the bottom end of the comb 31 and the part far from the crossbeam 32 is the top end of the comb 31. Each comb 31 forms a channel 33 with the crossbeam 32, and the distance between the combs 31 is the comb gap, i.e. the width of the channel 33. The size of the comb gap can be adjusted according to the actual thickness of the blade. In one embodiment, the comb gap is not less than 0.5 mm so that the blade can be inserted. In one embodiment, the length of the comb 31 is at least 23 mm so that the height of the comb 31 is not lower than the container 1 to accommodate a sample of appropriate size. Advantageously, the height of the comb 31 is higher than the container 1 so that the blade is aligned for cutting. The width of the comb 31 corresponds to the thickness of the sample slice, and the comb plate 3 with different comb 31 widths can be prepared according to the required thickness of the sample. The thinner the required sample, the narrower the width of the comb 31.
[0031] The following uses Figure 4 , Figure 5 are examples of specific dimensions of the comb plate 3.
[0032] In one embodiment: the comb plate 3 is prepared from the copper sheet 4. The comb plate 3 has 21 combs 31, wherein the comb 31 is 1 mm wide and 23 mm high, and the comb gap is 0.5 mm. The crossbeam 32 is 5 mm high.
[0033] In another embodiment: the comb plate 3 is prepared from the copper sheet 4. The comb plate 3 has 16 combs 31, wherein the comb 31 is 1.5 mm wide and 23 mm high, and the comb gap is 0.5 mm. The crossbeam 32 is 5 mm high.
[0034] The structure after inserting the comb plate 3 is shown in Figure 6 and Figure 7 Two pieces of the same comb plate 3 are inserted into the slots 22a and 22b of the base plate 2 in the container 1 respectively, with the top end of the comb 31 facing upwards, and the two comb plates 3 are placed in axial symmetry. The blade can be cut along the channel 33 from the top end of the comb to the bottom end of the comb, down to the crossbeam 32.
[0035] The exemplary operation steps are as follows: placing the bottom plate 2 into the container 1, and inserting the comb plate 3 into the insertion slot 22 of the bottom plate 2 respectively. After preparing 1% agarose gel, pour the agarose gel into the container 1, and stop pouring when the thickness is greater than 5 mm. After the bottom gel is solidified, place the sample, adjust the position, and continue to add the gel liquid until the sample is completely immersed in the gel liquid. Place the container 1 in the 4°C refrigerator for 30 min, and then take it out. Insert the prepared blade into the required channel 33 of the comb plate 3, and cut the sample with appropriate thickness.
[0036] The size of the container 1, the bottom plate 2 and the comb plate 3 in the present application can be adjusted according to actual needs. The above exemplary size can be suitable for most animal samples. Since the comb plate 3 can be cut to obtain appropriate combs 31 and channels 33, the device of the present application can be not limited to brain tissue and heart of experimental animals (such as mice and rats), and can also be suitable for precise cutting of other organs.
[0037] The above merely describes the preferred embodiments of the present application, but is not used to limit the protection scope of the present application.
Claims
1. A cutting device for animal specimen preparation, characterized in that The application relates to a container (1) made of organic glass, a bottom supporting plate (2) and a comb tooth plate (3). The container (1) has a bottom surface (11) and a side wall (12); the bottom supporting plate (2) has a bottom plate (23) and four supporting columns (21) standing on the four corners of the bottom plate (23) and being perpendicular to the bottom plate (23); a slot (22a) is arranged along one edge a of the bottom plate (23) and a slot (22b) is arranged along the opposite edge b of the bottom plate (23); the slots (22a) and (22b) are parallel to each other; the bottom supporting plate (2) is detachably arranged in the container (1) and the height of the supporting columns (21) is higher than the side wall (12) of the container (1); the bottom plate (23) and the supporting columns (21) are made of organic glass; and the comb tooth plate (3) has a crossbeam (32) and comb teeth (31) arranged on the crossbeam (32) at intervals; the comb tooth plate (3) is detachably inserted into the slots (22a) and (22b) through the crossbeam (32) respectively; the height of the comb teeth (31) is not lower than the side wall (12) of the container (1); the comb tooth plate (3) is made of copper; and the width of the comb teeth (31) corresponds to the thickness of a sample slice. The thickness of the comb tooth plate (3) is 1 mm. The distance between the slot (22a) and the edge a is 5 mm and the distance between the slot (22b) and the edge b is 5 mm.
2. The cutting device of claim 1, wherein, The inner diameter of the container (1) is 50 mm x 50 mm x 30 mm; the height of the supporting columns (21) is 35 mm; the height of the comb teeth (31) of the comb tooth plate (3) is 23 mm; and the height of the crossbeam (32) is 5 mm.
3. The cutting device of claim 2, wherein, The comb tooth plate (3) has 21 comb teeth (31); the width of the comb teeth (31) is 1 mm; and the interval between the comb teeth (31) is 0.5 mm.
4. The cutting device of claim 3, wherein, The comb tooth plate (3) has 16 comb teeth (31); the width of the comb teeth (31) is 1.5 mm; and the interval between the comb teeth (31) is 0.5 mm.
5. The cutting device of claim 4, wherein, 6. The cutting device of claim 4, wherein,