Autologous cartilage cell culture device for cartilage tissue repair

Abstract

The utility model discloses an autologous chondrocyte culture device for cartilage tissue repair, which comprises a culture device body and a culture shelf body, the culture device body comprises a box body, and a constant temperature control system is arranged in the box body; the culture shelf body comprises a plurality of placing plates, the placing plates are detachably mounted in the box body, a plurality of vent holes are formed in the placing plates, a plurality of connecting blocks are rotatably connected to the inner sides of the left side wall and the right side wall of the box body, sliding rails are arranged in the connecting blocks, and the sliding rails are arranged in the sliding rails. And sliding blocks are fixedly connected to the left end and the right end of the placement plate. The placing plate is rotatably mounted in the culture device, and when the culture dish needs to shake, the culture dish can be driven to shake through rotation of the placing plate, so that the shake of the culture dish does not need to be taken out from the culture device, and the culture dish is in a constant-temperature environment while being conveniently shaken; the culture of cartilage cells is facilitated.

Description

Technical Field

[0001] The utility model belongs to the technical field of chondrocyte culture, and in particular relates to an autologous chondrocyte culture device for repairing cartilage tissue. Background Art

[0002] Chondrocytes have different morphologies due to their location in the cartilage tissue. Research on them is very important for human health. In order to better study them, they need to be cultured, so they will be cultivated using the corresponding in vitro culture cabinet.

[0003] Existing autologous chondrocyte culture devices for cartilage tissue repair use a constant temperature control system to regulate the temperature within the culture device during culture. The culture racks within the culture device are usually fixedly connected so that the placement plates in the culture racks are fixed, thereby preventing the culture dishes placed on the placement plates from moving. Since the culture dishes need to be shaken from time to time to improve the growth efficiency of cells when culturing cartilage tissue within the culture device, the shaking of the culture dishes needs to be done by taking them out of the culture device, which makes shaking the culture dishes troublesome and causes the ambient temperature of the culture dishes to change, which is not conducive to the cultivation of chondrocytes.

[0004] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. Utility Model Content

[0005] The purpose of the utility model is to provide an autologous chondrocyte culture device for cartilage tissue repair, which can solve the problem that the culture dish in the culture device needs to be taken out and shaken, which causes shaking trouble and the environmental temperature change of the culture dish is not conducive to chondrocyte culture.

[0006] In order to achieve the above-mentioned purpose, the technical solution provided by a specific embodiment of the present invention is as follows:

[0007] An autologous chondrocyte culture device for cartilage tissue repair, comprising:

[0008] The culture device body includes a box body, wherein a constant temperature control system is provided in the box body, and the temperature in the box body is adjusted by the constant temperature control system so that the temperature in the box body is set to the temperature required for chondrocyte culture.

[0009] The culture shelf body comprises placing plates for placing culture dishes. The placing plates are provided in plurality to facilitate the layered storage of the culture dishes. The plurality of placing plates are detachably mounted in the box. The detachable placing plates allow the space in the box to be adjusted according to the requirements of use. The placing plates are provided with a plurality of air holes to allow the air to circulate in the box, so that the temperature in the box is uniform. The inner sides of the left and right side walls of the box are rotatably connected with a plurality of connecting blocks, so that the connecting blocks can be rotated when mounted on the inner side walls of the box. The connecting blocks are provided with sliding rails, and the left and right ends of the placing plates are fixedly connected with sliding blocks. The sliding blocks at the left and right ends of the placing plates are slidably connected in the sliding rails of the left and right connecting blocks, so that the placing plates are slidably connected in the sliding rails, and the placing plates are mounted on the connecting blocks, realizing the detachable connecting blocks. Since the connecting blocks are rotatable, the rotation of the connecting blocks can drive the rotation of the placing plates, so that the culture dishes can be shaken by the rotation of the placing plates, and the shaking of the culture dishes on the placing plates can be realized without taking them out of the box, so that the shaking of the culture dishes is convenient, and the environment of the culture dishes does not change when shaken, which is beneficial to the culture of chondrocytes.

[0010] In one or more embodiments of the present application, the front end of the box is provided with a switch door by hinging, and the opening and closing of the switch door facilitates the taking of the culture dishes in the box. The inner side wall of the switch door is provided with a sealing gasket, so that the sealing between the switch door and the box is good when the switch door is closed. The outer side wall of the switch door is fixedly connected with a handle, which facilitates the opening and closing of the switch door.

[0011] In one or more embodiments of the present application, the front and rear ends of the placing plates are provided with a certain distance from the front and rear side walls of the box. Since the placing plates are mounted in the box and rotated at a certain angle to shake the culture dishes on the placing plates, the front and rear ends of the placing plates are provided with a certain distance from the front and rear side walls of the box to facilitate the rotation of the placing plates in the box.

[0012] In one or more embodiments of the present application, the left and right side walls of the box are provided with a plurality of bearings, and the bearings are provided with rotating shafts. The bearings allow the rotating shafts to rotate.

[0013] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0014] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0015] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0016] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0017] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0018] In one or more embodiments of the utility model, the rotation shaft on the left side wall of the box is fixedly connected with a rotating block on the one end outside the box, so that the operating personnel can drive the placing plate to rotate by rotating the rotating block during use, and the rotation of the placing plate is convenient.

[0019] Compared with the prior art, the utility model installs the placing plate in the rotatable mode in the culture device, when the culture dish needs to be shaken, the culture dish can be shaken by the rotation of the placing plate, so that the culture dish is convenient to shake while being in the constant temperature environment, and the culture of the cartilage cells is facilitated. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced as follows. Obviously, the drawings in the following description only show some embodiments of the present application, and other drawings can also be obtained according to these drawings without any creative effort.

[0021] Figure 1 It is a perspective view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0022] Figure 2 It is an internal schematic view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0023] Figure 3 It is an internal perspective view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0024] Figure 4 It is a sectional view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0025] Figure 5 It is a front view sectional view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0026] Figure 6 It is a right view sectional view of the autologous chondrocyte culture device for cartilage tissue repair in an embodiment of the present application.

[0027] Figure 7 It is a schematic view of A in the embodiment of the present application. Figure 1

[0028] Figure 8 It is a schematic view of B in the embodiment of the present application. Figure 3

[0029] Main figure mark explanation:

[0030] 1-culture device body, 11-box body, 12-switch door, 13-sealing gasket, 14-handle, 2-culture rack body, 21-placing plate, 22-vent hole, 23-connection block, 24-sliding rail, 25-sliding block, 26-rotation shaft, 27-bearing, 28-rotation block, 29-limiting plate, 210-first limiting screw, 211-second limiting screw, 212-protection frame, 213-limiting rod. DETAILED DESCRIPTION

[0031] ​​In order to make the person skilled in the art better understand the technical scheme in the present application, the technical scheme in the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by the person skilled in the art without creative labor should belong to the scope of protection of the present application.

[0032] As shown in Figures 1-5 , the self-cultured cartilage cell culture device for cartilage tissue repair in an embodiment of the present application comprises a culture device body 1 and a culture rack body 2.

[0033] As shown in Figures 1-5 , the culture device body 1 comprises a box body 11, and a constant temperature control system is arranged in the box body 11. The temperature in the box body 11 is adjusted by the constant temperature control system, so that the temperature in the box body 11 is set at the temperature required for cartilage cell culture.

[0034] As shown in Figure 1 , a switch door 12 is hingedly installed at the front end of the box body 11. The opening and closing of the switch door 12 is convenient for taking the culture dishes in the box body 11.

[0035] As shown in Figure 1 and Figure 6 , a sealing gasket 13 is arranged on the inner side wall of the switch door 12, so that the sealing property between the switch door 12 and the box body 11 is good when the switch door 12 is closed. A handle 14 is fixedly connected to the outer side wall of the switch door 12, and the handle 14 is convenient for opening and closing the switch door 12.

[0036] As shown in Figures 2-6As shown, the culture shelf body 2 comprises a placing plate 21 for placing culture dishes. The placing plate 21 is provided in plurality to facilitate the layered storage of culture dishes by the plurality of placing plates 21. The plurality of placing plates 21 are detachably mounted in the box body 11, and the detachable placing plates 21 allow the space in the box body 11 to be adjusted according to the requirements of use. The placing plate 21 is provided with a plurality of air holes 22, which allow the placement of the placing plate 21 to not affect the circulation of air in the box body 11, so that the temperature in the box body 11 is uniform. The inner sides of the left and right side walls of the box body 11 are rotatably connected to a plurality of connecting blocks 23, so that the connecting blocks 23 can be rotated when mounted on the inner side walls of the box body 11. A plurality of sliding rails 24 are provided in the connecting blocks 23, and the left and right ends of the placing plate 21 are fixedly connected to sliding blocks 25, which are slidably connected to the sliding rails 24 in the left and right connecting blocks 23, so that the placing plate 21 is slidably connected to the sliding rails 24 through the sliding blocks 25, and the placing plate 21 is mounted on the connecting blocks 23, realizing the detachability of the placing plate 21. At the same time, since the connecting blocks 23 are rotatable, the rotation of the connecting blocks 23 can drive the placing plate 21 to rotate, so that the rotation of the placing plate 21 can drive the culture dishes to rotate, thereby facilitating the shaking of the culture dishes by rotating the placing plate 21 to drive the culture dishes to rotate, so that the shaking of the culture dishes can be realized without taking them out of the box body 11, thereby facilitating the shaking of the culture dishes and keeping the environment unchanged when the culture dishes are shaken, which is conducive to the culture of chondrocytes.

[0037] Preferably, the rotation of the placing plate 21 can drive all the culture dishes on the placing plate 21 to rotate, so that the shaking of the culture dishes can be facilitated at the same time as saving the shaking time.

[0038] As shown in Figure 6 , the front and rear ends of the placing plate 21 are spaced apart from the front and rear side walls of the box body 11 by a certain distance. Since the placing plate 21 is mounted in the box body 11 and rotated by a certain angle to drive the culture dishes on the placing plate 21 to shake, the front and rear ends of the placing plate 21 are spaced apart from the front and rear side walls of the box body 11 by a certain distance to facilitate the rotation of the placing plate 21 in the box body 11.

[0039] As shown in Figures 3-5 , Figure 7 , a plurality of bearings 27 are mounted on the left and right side walls of the box body 11, and a rotating shaft 26 is mounted in the bearings 27, so that the rotating shaft 26 can rotate through the bearings 27.

[0040] As shown in Figure 4 and Figure 5As shown, the end of the rotating shaft 26 placed in the box body 11 is fixedly connected to the side wall of the connecting block 23, so that the rotation of the connecting block 23 is realized through the rotation of the rotating shaft 26. The end of the rotating shaft 26 placed on the left side wall of the box body 11 is fixedly connected with the rotating block 28, so that the operator can rotate the placing plate 21 by rotating the rotating block 28 during use, and the rotation of the placing plate 21 is facilitated.

[0041] As shown in Figure 7 , the rotating shaft 26 on the left side wall of the box body 11 is fixedly connected with the limiting plate 29 on the side wall outside the box body 11, and the limiting plate 29 is attached to the outer side wall of the box body 11. When the rotating shaft 26 rotates, the limiting plate 29 can be rotated.

[0042] As shown in Figure 7 , the first limiting screw 210 is threadedly connected to the limiting plate 29, and the front end of the first limiting screw 210 abuts against the outer side wall of the box body 11. By abutting the front end of the first limiting screw 210 against the outer side wall of the box body 11, the rotation of the limiting plate 29 can be limited, thereby limiting the rotation of the rotating shaft 26, and finally limiting the rotation of the placing plate 21, so that the placing plate 21 is stable.

[0043] As shown in Figure 8 , the second limiting screw 211 is threadedly connected to the upper end wall plate of the connecting block 23, and the lower end of the second limiting screw 211 abuts against the upper side wall of the sliding block 25. The second limiting screw 211 can limit the sliding of the sliding block 25 in the sliding rail 24, so that the placing plate 21 is stable after installation.

[0044] As shown in Figure 2 and Figure 3 , the protective frame 212 is fixedly connected to the placing plate 21 and is arranged at the edge of the placing plate 21. In order to stabilize the culture dishes on the placing plate 21 during rotation, the protective frame 212 is arranged to prevent the culture dishes from falling off during rotation of the placing plate 21. In order to further ensure the stability of the culture dishes, a frame for limiting the culture dishes can be arranged on the placing plate 21, which facilitates the placement of the culture dishes and ensures the stability of the culture dishes after placement.

[0045] As shown in Figure 2 , Figure 5 and Figure 6As shown, the box 11 is fixedly connected with a pair of limit rods 213 on the left and right inner side walls below the connecting block 23, and one connecting block 23 is fixedly connected with a pair of limit rods 213, and the pair of limit rods 213 are respectively arranged on the front and rear sides below the connecting block 23, and since there is a certain distance between the limit rod 213 and the bottom of the connecting block 23, the connecting block 23 can rotate, and when the bottom of the connecting block 23 contacts the limit rod 213, the rotation of the connecting block 23 can be limited, so that the rotation range of the connecting block 23 is controlled through the limit rod 213, so that the placing plate 21 can rotate, and the culture dishes on the placing plate 21 will not fall off during rotation.

[0046] In use, the culture dishes containing chondrocytes are placed on the placing plate 21 in turn, and after the placing is completed, the switch door 12 is closed, the temperature in the box 11 is adjusted to the temperature required for chondrocyte culture through the temperature control system, when the culture dishes need to be shaken, the first limiting screw rod 210 is loosened, so that the first limiting screw rod 210 is separated from the side wall of the box 11, the rotating block 28 is rotated, so that the rotating shaft 26 is rotated, the rotating shaft 26 drives the connecting block 23 to rotate, and the connecting block 23 drives the placing plate 21 to rotate when rotating, and the rotation of the placing plate 21 shakes the culture dishes, and the limit rods 213 arranged at the bottom of the connecting block 23 limit the rotation range of the placing plate 21, so that the culture dishes will not fall off from the placing plate 21 when shaken by rotation, so that the shaking of the culture dishes is facilitated, and when the operating personnel manually rotates the rotating block 28, the rotating block 28 should be uniformly and slowly rotated to avoid falling off of the culture dishes on the placing plate 21 due to forceful rotation.

[0047] It is apparent for those skilled in the art that the present application is not limited to the details of the above-described exemplary embodiments, but can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered in all aspects as illustrative and not restrictive, and the scope of the present application is defined by the appended claims rather than the above description, and it is intended to encompass all changes falling within the meaning and range of equivalents of the elements of the claims. Any reference signs in the claims should not be considered as limiting the claims to which they relate.

[0048] In addition, it should be understood that although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description of the specification is only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can be appropriately combined to form other embodiments that those skilled in the art can understand.

Claims

1. An autologous chondrocyte culture device for cartilage tissue repair, characterized by, Include: Culture device body, including box, the box is provided with thermostatic control system; Culture frame body, including placing plate, the placing plate is provided with multiple, multiple placing plates are installed in the box in a detachable manner, multiple ventilation holes are opened on the placing plate, the inner side of the left and right side walls of the box is rotatably connected with multiple connecting blocks, multiple sliding rails are opened in the connecting blocks, the left and right ends of the placing plate are fixedly connected with sliding blocks, and the sliding blocks on the left and right ends of the placing plate are slidably connected in the sliding rails on the left and right connecting blocks.

2. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, The front end of the box is rotatably connected with an opening and closing door, the inner side wall of the opening and closing door is provided with a sealing gasket, and the outer side wall of the opening and closing door is fixedly connected with a handle.

3. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, The front and rear ends of the placing plate are arranged at a certain distance from the front and rear side walls of the box.

4. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, Multiple bearings are installed on the left and right side walls of the box, and rotating shafts are installed in the bearings.

5. The autologous chondrocyte culture device for cartilage tissue repair according to claim 4, wherein, One end of the rotating shaft placed in the box is fixedly connected to the side wall of the connecting block, and the other end of the rotating shaft placed outside the box is fixedly connected with a rotating block.

6. The autologous chondrocyte culture device for cartilage tissue repair according to claim 5, wherein, The rotating shaft on the left side wall of the box is fixedly connected with a limiting plate on the side wall outside the box.

7. The autologous chondrocyte culture device for cartilage tissue repair according to claim 6, wherein, The limiting plate is attached to the outer side wall of the box.

8. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, The first limiting screw is threadedly connected to the limiting plate, and the front end of the first limiting screw abuts against the outer side wall of the box.

9. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, The second limiting screw is threadedly connected to the upper end wall of the connecting block, and the lower end of the second limiting screw abuts against the upper side wall of the sliding block.

10. The autologous chondrocyte culture device for cartilage tissue repair according to claim 1, wherein, The placing plate is fixedly connected with a protective frame, and the protective frame is arranged at the edge of the placing plate. Multiple limiting rods are fixedly connected to the left and right side walls, and multiple limiting rods are arranged below the connecting blocks.

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