Rapid buffer solution replacement device

Abstract

The utility model discloses a quick buffer solution replacement device, which relates to the technical field of replacement devices and comprises a shell, a top cover is arranged at the top end of the shell, limiting grooves are symmetrically formed in the inner wall of the shell, and positioning grooves are formed in four corners of the top end of the shell. Positioning columns matched with the positioning grooves in shape are fixedly connected to the four corners of the bottom end of the top cover correspondingly, square grooves are symmetrically formed in the bottom end of the top cover, moving plates are slidably connected to the inner walls of the two square grooves correspondingly, and a plurality of springs are fixedly connected between the two moving plates and the square grooves correspondingly; through structures such as a feeding pipe, an annular ultrafiltration membrane, a motor, a belt transmission mechanism, a rotating cylinder, a circular column, a circular groove, a rotating ring, a placement column and a buffer solution inlet pipe, the effects of quickly replacing a buffer solution and improving the replacement efficiency are achieved; the problem that a traditional replacement device is low in overall replacement efficiency is solved.

Description

Technical Field

[0001] This utility model relates to the field of replacement device technology, and in particular to a rapid buffer solution replacement device. Background Technology

[0002] A rapid buffer replacement device is a device or tool specifically designed to quickly and effectively replace the original buffer system in a sample. These devices have wide applications in scientific research and biopharmaceutical fields, especially in scenarios requiring efficient processing of biomacromolecules such as proteins, antibodies, and enzymes. Rapid buffer replacement devices utilize specific techniques or methods, such as gel filtration chromatography, centrifugation, and ultrafiltration.

[0003] The existing buffer replacement device requires manual loading of buffer solution during the replacement process, which increases the operation time and results in low overall replacement efficiency. Furthermore, it is inconvenient to open the top cover of the device after replacement, making it impossible to thoroughly clean the inside of the device. This leads to residual impurities breeding bacteria, which affects the replacement operation on the lower side.

[0004] Therefore, this paper proposes a rapid buffer replacement device to solve the above-mentioned technical problems. Utility Model Content

[0005] The main objective of this invention is to provide a rapid buffer replacement device that can effectively solve the technical problems of low overall replacement efficiency and inability to thoroughly clean the inside of the device in the prior art.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A rapid buffer solution replacement device includes a housing, a top cover at the top of the housing, symmetrically spaced limiting grooves on the inner wall of the housing, positioning grooves at the four corners of the top of the housing, positioning posts with shapes adapted to the positioning grooves fixedly connected at the four corners of the bottom of the top cover, symmetrically spaced square grooves at the bottom of the top cover, movable plates slidably connected to the inner walls of two square grooves, multiple springs fixedly connected between the two sets of movable plates and the square grooves, limiting blocks fixedly connected to the two movable plates at opposite ends, the two limiting blocks being triangular in shape, and push posts fixedly connected above the corresponding limiting blocks at the opposite ends of the two movable plates, penetrating the top cover.

[0008] As a further embodiment of this utility model, a placement column is rotatably connected to the bottom of the inner wall of the outer shell, and multiple circular columns are fixedly connected to the top of the placement column. A rotating ring is rotatably connected to the bottom of the top cover, and multiple circular grooves adapted to the shape of the circular columns are opened at the bottom of the rotating ring.

[0009] As a further embodiment of this utility model, a rotating cylinder is fixedly connected to the top of the rotating ring and passes through a top cover. A motor is fixedly connected to one side of the top of the top cover, and the drive shaft on the drive end of the motor is connected to the rotating cylinder through a belt transmission mechanism.

[0010] As a further embodiment of this utility model, a circular cover is rotatably connected to the top of the rotating cylinder, and L-shaped fixing plates are symmetrically fixed to the outer wall of the circular cover, with both L-shaped fixing plates being fixedly connected to the top cover.

[0011] As a further embodiment of this utility model, the top of the circular cap is fixedly connected to a buffer solution inlet tube and a feed tube, respectively. The buffer solution inlet tube is used to pour in the buffer solution, and the feed tube is used to pour in the sample solution. The inner wall of the placement column is provided with an annular ultrafiltration membrane for filtering the sample solution.

[0012] As a further embodiment of this utility model, a circular hole is provided at the bottom of the inner wall of the placement column, the bottom end of the circular hole is connected to a sealing groove, and the bottom end of the sealing groove is connected to a discharge hole.

[0013] As a further embodiment of this utility model, a discharge pipe is fixedly connected to the middle of the bottom end of the outer shell, a lifting column slides through the discharge pipe, a sealing block adapted to the shape of the sealing groove is rotatably connected to the top of the lifting column, and a waste pipe is fixedly connected to one side of the bottom end of the outer shell corresponding to the discharge pipe.

[0014] As a further embodiment of this utility model, support columns are fixedly connected to the four corners of the bottom of the outer shell, and a base plate is fixedly connected between the four support columns. An electric push rod is fixedly inserted through the base plate, and the telescopic end of the electric push rod is fixedly connected to the lifting column.

[0015] The beneficial effects of this utility model are as follows:

[0016] By cooperating with the feed tube, annular ultrafiltration membrane, motor, belt drive mechanism, rotating cylinder, circular column, circular groove, rotating ring, placement column and buffer inlet tube, the buffer solution can be completely replaced. Since no manual intervention is required when adding buffer solution, the buffer solution can be replaced quickly, thus improving replacement efficiency.

[0017] With the cooperation of the electric push rod, lifting column, sealing block, sealing groove, circular hole, discharge hole and discharge pipe, the sample solution after replacement can be easily collected, so that the culture work can continue.

[0018] By cooperating with the push column, moving plate, limiting block, and limiting groove, the limiting of the top cover can be released, allowing the outer shell and the top cover to separate. This buffers the annular ultrafiltration membrane, preventing it from becoming clogged over time, thus improving filtration efficiency. The interior of the outer shell can also be cleaned, and the wastewater discharged from the waste pipe ensures the cleanliness of the interior, preventing bacterial growth and ensuring that it does not affect the next replacement of the buffer solution. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of a rapid buffer replacement device according to the present invention;

[0020] Figure 2 This is a bottom-view perspective view of a rapid buffer solution replacement device according to the present invention;

[0021] Figure 3 This is an exploded view of a rapid buffer solution replacement device according to the present invention;

[0022] Figure 4 This is a structural diagram of the top cover portion of a rapid buffer solution replacement device according to the present invention;

[0023] Figure 5 This is a cross-sectional view of the outer shell of a rapid buffer solution replacement device according to the present invention;

[0024] Figure 6 for Figure 5 A magnified view of middle A;

[0025] Figure 7 This is another perspective view of the cross-sectional view of the outer shell of the rapid buffer replacement device of this utility model.

[0026] In the diagram: 1. Outer shell; 2. Top cover; 3. Limiting groove; 4. Positioning groove; 5. Positioning column; 6. Square groove; 7. Spring; 8. Moving plate; 9. Limiting block; 10. Push column; 11. Placement column; 12. Circular column; 13. Rotating ring; 14. Circular groove; 15. Rotating cylinder; 16. Motor; 17. Belt drive mechanism; 18. Circular cover; 19. L-shaped fixing plate; 20. Buffer solution inlet pipe; 21. Feed pipe; 22. Annular ultrafiltration membrane; 23. Sealing groove; 24. Discharge hole; 25. Sealing block; 26. Lifting column; 27. Electric push rod; 28. Discharge pipe; 29. ​​Waste pipe; 30. Base plate; 31. Support column; 32. Circular hole. Detailed Implementation

[0027] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0028] like Figure 1-7 As shown, a rapid buffer replacement device includes a housing 1, a top cover 2 at the top of the housing 1, symmetrically formed limiting grooves 3 on the inner wall of the housing 1, positioning grooves 4 at the four corners of the top of the housing 1, positioning posts 5 with shapes adapted to the positioning grooves 4 fixedly connected at the four corners of the bottom of the top cover 2, symmetrically formed square grooves 6 at the bottom of the top cover 2, movable plates 8 slidably connected to the inner walls of the two square grooves 6, multiple springs 7 fixedly connected between the two sets of movable plates 8 and the square grooves 6, limiting blocks 9 fixedly connected to the two movable plates 8 at opposite ends, the two limiting blocks 9 being triangular in shape, and push posts 10 fixedly connected above the limiting blocks 9 at the opposite ends of the two movable plates 8 and penetrating the top cover 2.

[0029] In this embodiment, a placement column 11 is rotatably connected to the bottom of the inner wall of the outer shell 1, and a plurality of circular columns 12 are fixedly connected to the top of the placement column 11. A rotating ring 13 is rotatably connected to the bottom of the top cover 2. A plurality of circular grooves 14 adapted to the shape of the circular columns 12 are opened at the bottom of the rotating ring 13. Through the cooperation of the circular grooves 14 and the circular columns 12, the rotating ring 13 can rotate the placement column 11 and generate centrifugal force.

[0030] In this embodiment, a rotating cylinder 15 is fixedly connected to the top of the rotating ring 13 and passes through the top cover 2. A motor 16 is fixedly connected to one side of the top of the top cover 2. The drive shaft on the drive end of the motor 16 is connected to the rotating cylinder 15 through a belt transmission mechanism 17. When the motor 16 is started, it drives the rotating cylinder 15 to rotate through the belt transmission mechanism 17, providing a power source for rotation. The two pulleys of the belt transmission mechanism 17 are fixedly connected to the drive shafts on the rotating cylinder 15 and the motor 16, respectively, and the two pulleys are connected by a belt.

[0031] In this embodiment, a circular cover 18 is rotatably connected to the top of the rotating cylinder 15. An L-shaped fixing plate 19 is symmetrically fixed to the outer wall of the circular cover 18. Both L-shaped fixing plates 19 are fixedly connected to the top cover 2. The circular cover 18 can always remain fixed by the L-shaped fixing plates 19.

[0032] In this embodiment, a buffer inlet tube 20 and a feed tube 21 are fixedly connected to the top of the circular cap 18. The buffer inlet tube 20 is used to pour in the buffer solution, and the feed tube 21 is used to pour in the sample solution. An annular ultrafiltration membrane 22 is provided on the inner wall of the placement column 11 and is used to filter the sample solution.

[0033] In this embodiment, a circular hole 32 is provided at the bottom of the inner wall of the placement column 11. The bottom end of the circular hole 32 is connected to a sealing groove 23, and the bottom end of the sealing groove 23 is connected to a discharge hole 24. The sample solution after the replacement buffer can be discharged and collected through the circular hole 32 and the discharge hole 24, which facilitates the subsequent culture work.

[0034] In this embodiment, a discharge pipe 28 is fixedly connected to the middle of the bottom end of the outer shell 1. A lifting column 26 slides through the discharge pipe 28. A sealing block 25 that matches the shape of the sealing groove 23 is rotatably connected to the top of the lifting column 26. A waste pipe 29 is fixedly connected to the bottom end of the outer shell 1 on the side corresponding to the discharge pipe 28. When the sealing block 25 and the sealing groove 23 are closed, the circular hole 32 is sealed to prevent leakage during centrifugation filtration. When the sealing block 25 and the sealing groove 23 are separated, the sample solution after the replacement buffer is poured out. The lifting column 26 and the sealing block 25 are rotatably connected. Therefore, when the placement column 11 rotates, it will rotate synchronously with the sealing block 25, reducing the friction in the sealing groove 23.

[0035] In this embodiment, support columns 31 are fixedly connected to the four corners of the bottom of the outer shell 1, and a base plate 30 is fixedly connected between the four support columns 31. An electric push rod 27 is fixedly passed through the base plate 30. The telescopic end of the electric push rod 27 is fixedly connected to the lifting column 26. The electric push rod 27 is limited by the support columns 31 and the base plate 30, so as to provide power for the lifting column 26 to rise and fall.

[0036] It should be noted that this utility model is a rapid buffer replacement device. In use, the sample solution can be placed into the annular ultrafiltration membrane 22 through the feed tube 21. At this time, the motor 16 is started and drives the rotating cylinder 15 to rotate through the belt drive mechanism 17. Through the cooperation of the circular column 12 and the circular groove 14, the rotating ring 13 can drive the placement column 11 and the annular ultrafiltration membrane 22 to rotate. This allows the sample solution in the annular ultrafiltration membrane 22 to rotate under the action of centrifugal force, thereby filtering out the buffer solution and impurities in the sample solution. Then, the buffer solution can be placed into the annular ultrafiltration membrane 22 through the buffer inlet tube 20, thereby completely replacing the buffer solution. Since no manual intervention is required when adding the buffer solution, the buffer solution can be replaced quickly, improving the replacement efficiency.

[0037] After the replacement buffer is activated, the electric push rod 27 moves the lifting column 26 downward, thereby moving the sealing block 25 downward so that it leaves the sealing groove 23. At this time, the sample solution of the replacement buffer falls from the circular hole 32 and is discharged through the discharge hole 24 and the discharge pipe 28. The sample solution after replacement is collected so that the culture work can continue.

[0038] Furthermore, after the overall replacement is completed, the push column 10 can be squeezed inward and moved towards the moving plate 8 and the limiting block 9, thereby causing the limiting block 9 to leave the range of the limiting groove 3, releasing the limiting of the top cover 2, so that the outer shell 1 and the top cover 2 can be separated. Therefore, the annular ultrafiltration membrane 22 can be buffered to prevent the annular ultrafiltration membrane 22 from clogging over a long period of time, thereby improving the filtration efficiency. The inside of the outer shell 1 can also be cleaned, and the wastewater after cleaning is discharged from the waste pipe 29, ensuring the cleanliness of the inside of the outer shell 1, preventing the growth of bacteria and avoiding affecting the next replacement treatment of the buffer solution.

[0039] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A rapid buffer exchange device, comprising a housing (1), characterized in that: The top of the outer shell (1) is provided with a top cover (2). The inner wall of the outer shell (1) is symmetrically provided with limiting grooves (3). The top of the outer shell (1) is provided with positioning grooves (4) at the four corners. The bottom of the top cover (2) is fixedly connected with positioning posts (5) that are adapted to the shape of the positioning grooves (4). The bottom of the top cover (2) is symmetrically provided with square grooves (6). The inner walls of the two square grooves (6) are slidably connected with moving plates (8). Multiple springs (7) are fixedly connected between the two sets of moving plates (8) and the square grooves (6). The two moving plates (8) are fixedly connected with limiting blocks (9) at one end away from each other. The two limiting blocks (9) are triangular in shape. The upper part of the two moving plates (8) corresponding to the limiting blocks (9) is fixedly connected with a push post (10) that penetrates the top cover (2).

2. The rapid buffer replacement device according to claim 1, characterized in that: The bottom of the inner wall of the outer shell (1) is rotatably connected to a placement column (11), and the top of the placement column (11) is fixedly connected to a plurality of circular columns (12). The bottom of the top cover (2) is rotatably connected to a rotating ring (13), and the bottom of the rotating ring (13) is provided with a plurality of circular grooves (14) that are adapted to the shape of the circular columns (12).

3. The rapid buffer replacement device according to claim 2, characterized in that: The top of the rotating ring (13) is fixedly connected to a rotating cylinder (15) and passes through a top cover (2). A motor (16) is fixedly connected to one side of the top of the top cover (2). The drive shaft on the drive end of the motor (16) and the rotating cylinder (15) are connected through a belt transmission mechanism (17).

4. The rapid buffer replacement device according to claim 3, characterized in that: The top of the rotating cylinder (15) is rotatably connected to a circular cover (18), and the outer wall of the circular cover (18) is symmetrically fixedly connected to an L-shaped fixing plate (19), and both L-shaped fixing plates (19) are fixedly connected to the top cover (2).

5. A rapid buffer replacement device according to claim 4, characterized in that: The top of the circular cap (18) is fixedly connected to a buffer inlet tube (20) and a feed tube (21). The buffer inlet tube (20) is used to pour in the buffer solution, and the feed tube (21) is used to pour in the sample solution. The inner wall of the placement column (11) is provided with an annular ultrafiltration membrane (22) for filtering the sample solution.

6. A rapid buffer replacement device according to claim 2, characterized in that: A circular hole (32) is provided at the bottom of the inner wall of the placement column (11). The bottom end of the circular hole (32) is connected to a sealing groove (23), and the bottom end of the sealing groove (23) is connected to a discharge hole (24).

7. A rapid buffer replacement device according to claim 6, characterized in that: A discharge pipe (28) is fixedly connected to the middle of the bottom end of the outer shell (1). A lifting column (26) slides through the discharge pipe (28). A sealing block (25) that matches the shape of the sealing groove (23) is rotatably connected to the top of the lifting column (26). A waste pipe (29) is fixedly connected to one side of the bottom end of the outer shell (1) corresponding to the discharge pipe (28).

8. A rapid buffer replacement device according to claim 7, characterized in that: Support columns (31) are fixedly connected to the four corners of the bottom of the outer shell (1). A base plate (30) is fixedly connected between the four support columns (31). An electric push rod (27) is fixedly inserted through the base plate (30). The telescopic end of the electric push rod (27) is fixedly connected to the lifting column (26).

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