Magnetic ultrasonic device and nucleic acid detection analyzer

Abstract

The utility model discloses a magnetic suction ultrasonic device and a nucleic acid detection analyzer. The magnetic suction ultrasonic device comprises an ultrasonic assembly and a driving assembly, the ultrasonic assembly comprises a sleeve and an ultrasonic part movably inserted into the sleeve in the height direction, and the ultrasonic end of the ultrasonic part extends out of the top end of the sleeve. A press-fitting plate is mounted at the driving end of the driving assembly, a magnetic attraction part is arranged on the press-fitting plate, and the driving assembly is used for driving the press-fitting plate to move between a first position and a second position; at the first position, the press-fitting plate presses the ultrasonic part downwards, so that the ultrasonic end is separated from the bottom of the external consumable card box; at the second position, the press-fitting plate is separated from the ultrasonic transducer, so that the ultrasonic end abuts against the bottom of an external consumable clamping box, and the magnetic attraction piece attracts the magnetic beads in the consumable clamping box from the periphery. According to the device, internal magnetic beads can be adsorbed while a sample in the consumable is subjected to ultrasonic treatment, and the press-fitting plate is driven to move through the driving assembly, so that ultrasonic positioning of the consumable clamping box is achieved.

Description

Technical Field

[0001] This application belongs to the field of nucleic acid detection technology, specifically relating to a magnetic ultrasonic device and a nucleic acid detection analyzer. Background Technology

[0002] Nucleic acid amplification detection technology is one of the important methods for target detection in industries such as clinical diagnosis and quarantine inspection. Multiplex nucleic acid amplification detection technology amplifies multiple nucleic acid targets simultaneously through a single nucleic acid amplification reaction, and then detects the amplified products using certain detection methods to achieve diagnosis of multiple targets. It has the characteristics of high efficiency and high throughput and is widely used in fields such as pathogen diagnosis.

[0003] Rapid and accurate pathogen detection is a crucial step in the prevention and treatment of infectious diseases; therefore, pathogen nucleic acid detection plays an indispensable role in pathogen diagnosis. Before testing nucleic acid samples, the cells in the sample within the consumable cartridge need to be sonicated. However, since a single consumable cartridge can contain multiple cartridges, manual positioning is usually used during sonication. Furthermore, the presence of magnetic beads within the consumable cartridge may affect the sonication results. Utility Model Content

[0004] The purpose of this application is to provide a magnetic ultrasound device and a nucleic acid detection analyzer that can perform ultrasound and magnetic attraction simultaneously, and achieve automatic ultrasound positioning during the ultrasound process.

[0005] To achieve the above objectives, this application provides a magnetic suction ultrasound device, comprising:

[0006] An ultrasonic assembly includes a sleeve and an ultrasonic component that is movably inserted into the sleeve along the height direction, with the ultrasonic end of the ultrasonic component extending from the top of the sleeve.

[0007] The drive assembly has a pressure plate mounted on its drive end. The pressure plate is equipped with a magnetic suction element. The drive assembly is used to drive the pressure plate to move between a first position and a second position.

[0008] In the first position, the pressing plate presses down on the ultrasonic component to detach the ultrasonic end from the bottom of the external consumable card box; in the second position, the pressing plate detaches from the ultrasonic component so that the ultrasonic end abuts against the bottom of the external consumable card box, and the magnetic suction component attracts the magnetic beads inside the consumable card box from the outer periphery.

[0009] In some embodiments, the magnetic ultrasonic device further includes a mounting base, a drive assembly disposed on the mounting base, and a position detection element disposed on the mounting base for detecting the downward displacement of the pressure plate.

[0010] In some implementations, the driving component includes:

[0011] Rotary drive component;

[0012] A rotating lead screw is mounted on the drive end of a rotating drive component. One end of a pressure plate is connected to the rotating lead screw, and the other end is positioned near a position detection component.

[0013] In some embodiments, the drive assembly further includes a linear guide rail disposed on the mounting base, the linear guide rail extending in the height direction, and the side end face of the press plate slidingly engaging with the linear guide rail.

[0014] In some embodiments, a clearance opening is formed on the press plate, and the sleeve passes through the clearance opening.

[0015] In some embodiments, the magnetic attractor is a vertically extending block structure, and the side of the magnetic attractor facing the sleeve is the magnetic attractor surface.

[0016] In some embodiments, a drive component is provided at the bottom end of the sleeve, and an elastic element is elastically connected between the drive component and the bottom of the ultrasonic component. In the first position, the drive component and the pressure plate cooperate to adjust the deformation of the elastic element.

[0017] In some embodiments, the top end of the sleeve is provided with an end cap, and the end cap has a through hole. The ultrasonic component includes an extension and a piston connected sequentially along the height direction. The piston is movably disposed inside the sleeve and its bottom is elastically connected to an elastic element. The extension extends out from the through hole.

[0018] In some embodiments, the sleeve has an elongated hole that extends along the height of the sleeve. The ultrasonic component includes a guide portion located on the outer periphery of the piston portion. A guide protrusion is formed on the guide portion and extends movably from the elongated hole. The guide protrusion can move along the elongated hole under the action of an external driving force.

[0019] A second aspect of this application provides a nucleic acid detection analyzer, including the magnetic suction ultrasound device described above.

[0020] Through the above technical solution, the ultrasonic component of this application includes a sleeve and an ultrasonic component movably inserted into the sleeve along the height direction, with the ultrasonic end of the ultrasonic component extending from the top of the sleeve. A pressure plate is mounted on the driving end of the driving component, and a magnetic suction element is provided on the pressure plate. The driving component is used to drive the pressure plate to move between a first position and a second position. In the first position, the driving component drives the pressure plate downward to press down on the ultrasonic component, causing the ultrasonic end to detach from the bottom of the external consumable cartridge. In the second position, the driving component drives the pressure plate upward and disengages from the ultrasonic transducer, causing the ultrasonic end to abut against the bottom of the external consumable cartridge, thereby achieving automatic positioning of the consumable cartridge during ultrasonication. Furthermore, during ultrasonication, the magnetic suction element attracts magnetic beads from the outer periphery of the consumable cartridge, gathering the magnetic beads together to prevent interference with the ultrasonic effect of the ultrasonic component on the sample inside the consumable cartridge.

[0021] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description

[0022] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0023] Figure 1 This is a schematic diagram of the magnetic ultrasonic device of this application during its working process;

[0024] Figure 2 This is a schematic diagram of the magnetic ultrasonic device of this application;

[0025] Figure 3 This is a front view schematic diagram of the magnetic ultrasonic device of this application;

[0026] Figure 4 for Figure 3 Cross-sectional view of the BB region.

[0027] Description of Reference Numerals

[0028] 10 Ultrasonic components 20 Drive components

[0029] 11 Sleeve 21 Rotary drive component

[0030] 111 Elongated hole 22 Rotating lead screw

[0031] 12 Ultrasonic components 23 Linear guide rail

[0032] 121 Extension section 30 Press-fit plate

[0033] 122 Piston section 31 Clearance opening

[0034] 123 Guide protrusion 40 Position detection component

[0035] 13 End caps 50 Consumable card holder

[0036] 14 Elastic element 60 Mounting base

[0037] 15 Drive components 70 Magnetic suction components Detailed Implementation

[0038] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.

[0039] The magnetic ultrasonic device and nucleic acid detection analyzer according to this application are described below with reference to the accompanying drawings.

[0040] like Figure 1 and Figure 2 As shown, this application provides a magnetic ultrasonic device, including an ultrasonic component 10 and a driving component 20. The ultrasonic component 10 includes a sleeve 11 and an ultrasonic component 12 that is movably inserted into the sleeve 11 along the height direction. The ultrasonic end of the ultrasonic component 12 extends from the top of the sleeve 11. A pressure plate 30 is installed on the driving end of the driving component 20. A magnetic suction element 70 is provided on the pressure plate 30. The driving component 20 is used to drive the pressure plate 30 to move between a first position and a second position. In the first position, the pressure plate 30 presses down on the ultrasonic component 12 to disengage the ultrasonic end from the bottom of the external consumable card box 50. In the second position, the pressure plate 30 disengages from the ultrasonic component 12 to abut the bottom of the external consumable card box 50, and the magnetic suction element 70 attracts the magnetic beads inside the consumable card box 50 from the outer periphery.

[0041] Preferably, the first position is located below the second position. When the pressure plate 30 is in the first position, downward pressure is applied to the ultrasonic component 12, causing the ultrasonic end of the ultrasonic component 12 to move downward and disengage from the bottom of the consumable cartridge 50. When the drive assembly 20 drives the pressure plate 30 upward, the pressure plate 30 stops applying pressure to the ultrasonic component 12, and the ultrasonic end of the ultrasonic component 12 moves upward until it abuts against the bottom of the consumable cartridge 50, thereby achieving automatic ultrasonic positioning of the consumable cartridge 50 and improving the ultrasonic effect. Since the consumable cartridge 50 has a receiving cavity inside, which contains a sample and is also provided with magnetic beads, when the ultrasonic end of the ultrasonic component 12 abuts against the bottom of the consumable cartridge 50, the magnetic suction component 70 applies an attraction force to the magnetic beads in the receiving cavity, causing the magnetic beads to gather together, and the ultrasonic end of the ultrasonic component 12 performs ultrasound on the sample in the consumable cartridge 50. This application utilizes the cooperation of the drive assembly 20, the pressure plate 30, and the magnetic suction component 70 to gather magnetic beads together, thereby preventing any impact on the ultrasonic effect of the ultrasonic component 12 on the sample in the consumable cartridge 50, and enabling ultrasonication of samples at specific holes in the consumable cartridge 50.

[0042] like Figure 1As shown, the bottom of the consumable cartridge 50 has multiple holes for installing consumables. During ultrasound treatment, the sample inside the consumable above the ultrasound component 12 can be ultrasounded by moving the ultrasound component 12 in the height direction. When ultrasounding different consumables, it is only necessary to control the consumable cartridge 50 to move above the ultrasound component 12.

[0043] In some embodiments, the magnetic ultrasonic device further includes a mounting base 60, a drive assembly 20 is disposed on the mounting base 60, and a position detection element 40 is provided on the mounting base 60 for detecting the downward displacement of the pressure plate 30.

[0044] The position detection component 40 can be a photoelectric switch, which is positioned below the pressure plate 30. When the pressure plate 30 moves between the first and second positions along the height direction, the pressure plate 30 passes the photoelectric switch. The photoelectric switch senses the displacement of the pressure plate 30 in the height direction and emits a corresponding photoelectric sensing signal, thereby enabling the detection of the downward displacement of the pressure plate 30.

[0045] In some embodiments, the drive assembly 20 includes a rotary drive 21 and a rotary lead screw 22; the rotary lead screw 22 is threadedly rotatably disposed on the drive end of the rotary drive 21, one end of the pressure plate 30 is connected to the rotary lead screw 22, and the other end is disposed near the position detection member 40.

[0046] Among them, the rotary drive component 21 is a lead screw motor. In the initial position, the lead screw motor drives the rotating lead screw 22 to rotate, thereby driving the pressing plate 30 to move downward until the photoelectric switch is blocked. Then the photoelectric switch can emit a photoelectric signal and obtain the displacement of the pressing plate 30.

[0047] Furthermore, to ensure the stability of the pressing plate 30 when moving up and down along the height direction, the drive assembly 20 also includes a linear guide rail 23 provided on the mounting base 60. The linear guide rail 23 extends along the height direction, and the side end face of the pressing plate 30 slides in cooperation with the linear guide rail 23. When the rotating screw 22 drives the pressing plate 30 to move up and down, the pressing plate 30 simultaneously slides along the linear guide rail 23, thereby ensuring the smooth and precise movement of the pressing plate 30 and preventing horizontal swaying.

[0048] In some embodiments, a clearance opening 31 is formed on the pressing plate 30, through which the sleeve 11 passes. The clearance opening 31 is an arc-shaped concave notch, allowing the sleeve 11 to pass through, and a certain gap is maintained between the inner peripheral wall of the clearance opening 31 and the outer peripheral wall of the sleeve 11. Since the magnetic suction member 70 is fixed to the edge of the pressing plate 30 near the clearance opening 31, a small gap exists between the magnetic suction member 70 and the outer peripheral wall of the consumable card holder 50. When the ultrasonic component 12 abuts against the bottom of the consumable card holder 50, the strong magnetic force can gather the magnetic beads inside the consumable card holder 50 together, preventing any impact on the ultrasonic effect on the sample inside the consumable card holder 50.

[0049] In some embodiments, the magnetic attractor 70 is a vertically extending block structure, and the side of the magnetic attractor 70 facing the sleeve 11 is the magnetic attracting surface. Preferably, the magnetic attractor 70 is a magnet, and the magnetic attracting surface of the magnet is disposed facing the outer peripheral wall of the sleeve 11. In this way, when the ultrasonic end of the ultrasonic component 12 comes into contact with the consumable, the magnet applies a strong magnetic attraction force to the magnetic beads in the consumable, causing the magnetic beads to gather on the side close to the magnet.

[0050] like Figure 3 and Figure 4 As shown, a driving component 15 is provided at the bottom end of the sleeve 11. An elastic element 14 is elastically connected between the driving component 15 and the bottom of the ultrasonic component 12. In the first position, the driving component 15 and the pressure plate 30 cooperate to adjust the deformation of the elastic element 14. The elastic element 14 is a spring in the prior art, and the ultrasonic component 12 is an ultrasonic transducer in the prior art.

[0051] In this embodiment, a storage cavity is formed inside the sleeve 11. The top end of the ultrasonic component 12 is used to contact the external consumables and to ultrasonically break up the sample stored inside the consumables. The ultrasonic component 12 is disposed in the storage cavity and can move relative to the sleeve 11 under the action of the elastic member 14. When the ultrasonic component 12 moves downward relative to the sleeve 11, it can separate from the consumables. At this time, the ultrasonic component 12 can be horizontally adjusted so that the top of the ultrasonic component 12 and the bottom of the consumables can be efficiently positioned and engaged. Then, the ultrasonic component 12 is driven to move upward relative to the sleeve 11, and the ultrasonic component 12 can re-abut against the consumables, ensuring that the ultrasonic component 12 has a good ultrasonic breaking effect. A driving component 15 is provided at the bottom end of the sleeve 11. An elastic element 14 is elastically compressed between the driving component 15 and the bottom end of the ultrasonic component 12. By moving the driving component 15 and the pressure plate 30 up and down along the height direction, the deformation of the elastic element 14 can be adjusted. This allows the elastic element 14 to press the ultrasonic component 12, achieving the optimal ultrasonic fragmentation state and improving the ultrasonic fragmentation effect. In this embodiment, the ultrasonic effect transmitted from the ultrasonic component 12 to the consumable is different under different pressures. By driving the elastic element 14 with the driving component 15, the deformation of the elastic element 14 can be adjusted, enabling the elastic element 14 to transmit pressure to the ultrasonic component 12. The ultrasonic component 12 bears different pressures under different deformations of the elastic element 14. Through multiple adjustments, the ultrasonic component 12 can be driven to achieve the optimal ultrasonic fragmentation state.

[0052] In some embodiments, the top end of the sleeve 11 is provided with an end cap 13, and the end cap 13 has a through hole. The ultrasonic component 12 includes an extension 121 and a piston 122 connected sequentially along the height direction. The piston 122 is movably disposed inside the sleeve 11 and its bottom is elastically connected to the elastic member 14. The extension 121 extends out from the through hole.

[0053] The end cap 13 has a through hole in the middle. Preferably, the size of the extension 121 matches the size of the through hole, allowing the extension 121 to extend out of the through hole. This avoids gaps between the extension 121 and the through hole due to poor fit, which could allow external debris to easily enter the storage cavity and affect the normal operation of the ultrasonic component 12. The bottom of the piston 122 is elastically connected to the elastic member 14, allowing the piston 122 to respond more sensitively to the force generated during the elastic deformation of the elastic member 14. Thus, when the piston 122 moves downward to compress the elastic member 14 via the guide protrusion 123, the elastic member 14 will exert an upward force on the piston 122 when the force applied to the guide protrusion 123 disappears, thereby causing the piston 122 to move upward and positioning the extension 121 with the bottom of the consumable.

[0054] Furthermore, the top surface of the extension 121 has a receiving groove that adapts to the bottom of the consumable. The top of the extension 121 can be designed to conform to the shape of the consumable, which facilitates the transfer of more energy to the consumable. With the receiving groove, the bottom of the consumable can fully contact the top of the extension 121, improving the ultrasound transmission effect and also limiting the consumable, preventing it from detaching from the extension 121 during ultrasound operation due to poor fit, thus ensuring the normal operation of the sample cell ultrasound disruption.

[0055] In some embodiments, the sleeve 11 has an elongated hole 111 extending along the height direction of the sleeve 11. The ultrasonic component 12 includes a guide portion located on the outer periphery of the piston portion 122. A guide protrusion 123 is formed on the guide portion and extends movably from the elongated hole 111. The guide protrusion 123 can move along the elongated hole 111 under the action of an external driving force, thereby driving the piston portion 122 connected to the guide portion to move together. The guide protrusion 123 is located below the pressure plate 30. When the pressure plate 30 moves downward, it presses down the guide protrusion 123, causing the piston portion 122 to press against the elastic member 14. After adjusting the position relative to the consumable, it is released. Under the elastic recovery of the elastic member 14, the guide protrusion 123 moves upward along the elongated hole 111, and the piston portion 122 drives the extension portion 121 to move upward together, so that the extension portion 121 docks with the bottom of the consumable.

[0056] Furthermore, two elongated holes 111 are formed on the sleeve 11, and the two elongated holes 111 are symmetrically arranged on the side wall of the sleeve 11. Similarly, a guide protrusion 123 is symmetrically provided on each side of the guide portion. During the process of aligning the extension portion 121 with the bottom of the consumable, the pressing plate 30 first moves downward and simultaneously presses down the guide protrusions 123 on both sides, causing the guide protrusions 123 to move along the elongated holes 111, thereby driving the piston portion 122 to move downward, thereby compressing the elastic element 14 so that the elastic element 14 is in a compressed state. At this time, the extension portion 121 is not in contact with the bottom of the consumable, so the position of the sleeve 11 can be adjusted horizontally to allow the extension portion 121 to better cooperate with the bottom of the consumable. After the adjustment is completed, the pressing plate 30 is controlled to move upward and disengage from the guide protrusions 123. Under the action of the elastic element 14 elastic recovery, the guide protrusions 123 move upward along the elongated holes 111, and the piston portion 122 drives the extension portion 121 to move upward together, so that the extension portion 121 aligns with the consumable.

[0057] In other embodiments, the outer periphery of the drive component 15 is threadedly rotated with the inner peripheral wall of the sleeve 11. By rotating the drive component 15, the compression amount of the elastic element 14 can be adjusted, and the ultrasonic breaking effect of the ultrasonic component 12 under different compression amounts can be tested experimentally, thereby adjusting the ultrasonic component 12 to the optimal ultrasonic breaking state.

[0058] In this embodiment, the sleeve 11 is made of plastic, which is lightweight and has high structural strength while achieving good weight reduction. It can effectively transmit the pressure of the elastic element 14 without being easily crushed.

[0059] A second aspect of this application provides a nucleic acid detection analyzer, including the magnetic suction ultrasound device described above.

[0060] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0061] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0062] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0063] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A magnetically aspirated ultrasonic device, characterized in that, include: An ultrasonic component (10) includes a sleeve (11) and an ultrasonic component (12) that is movably inserted into the sleeve (11) along the height direction, wherein the ultrasonic end of the ultrasonic component (12) extends from the top of the sleeve (11). A drive assembly (20) is provided with a pressure plate (30) mounted on its drive end. The pressure plate (30) is provided with a magnetic suction element (70). The drive assembly (20) is used to drive the pressure plate (30) to move between a first position and a second position. In the first position, the pressing plate (30) presses down on the ultrasonic component (12) to disengage the ultrasonic end from the bottom of the external consumable card box (50); in the second position, the pressing plate (30) disengages from the ultrasonic component (12) to abut the bottom of the ultrasonic end from the bottom of the external consumable card box (50), and the magnetic suction member (70) attracts the magnetic beads inside the consumable card box (50) from the outer periphery.

2. The magnetic ultrasonic device according to claim 1, characterized in that, The magnetic ultrasonic device also includes a mounting base (60), the driving component (20) is disposed on the mounting base (60), and the mounting base (60) is provided with a position detection component (40), which is used to detect the downward displacement of the pressure plate (30).

3. The magnetic suction ultrasonic device according to claim 2, characterized in that, The driving component (20) includes: Rotary drive component (21); A rotating lead screw (22) is threadedly mounted on the drive end of the rotating drive member (21). One end of the press plate (30) is connected to the rotating lead screw (22), and the other end is located near the position detection member (40).

4. The magnetic suction ultrasonic device according to claim 2, characterized in that, The drive assembly (20) further includes a linear guide rail (23) disposed on the mounting base (60), the linear guide rail (23) extending in the height direction, and the side end face of the pressure plate (30) slidingly engaging with the linear guide rail (23).

5. The magnetic suction ultrasonic device according to claim 1, characterized in that, An clearance opening (31) is formed on the press plate (30), and the sleeve (11) is disposed through the clearance opening (31).

6. The magnetic suction ultrasonic device according to claim 1, characterized in that, The magnetic attractor (70) is a vertically extending block structure, and the side of the magnetic attractor (70) facing the sleeve (11) is the magnetic attractor surface.

7. The magnetic suction ultrasonic device according to any one of claims 1 to 6, characterized in that, The bottom end of the sleeve (11) is provided with a driving component (15), and an elastic element (14) is connected between the bottom of the driving component (15) and the ultrasonic component (12). In the first position, the driving component (15) and the pressure plate (30) cooperate to adjust the deformation of the elastic element (14).

8. The magnetic ultrasonic device according to claim 7, characterized in that, The top end of the sleeve (11) is provided with an end cap (13), and the end cap (13) has a through hole. The ultrasonic component (12) includes an extension (121) and a piston (122) connected sequentially along the height direction. The piston (122) is movably disposed inside the sleeve (11) and its bottom is connected to the elastic member (14). The extension (121) extends out from the through hole.

9. The magnetic suction ultrasonic device according to claim 8, characterized in that, The sleeve (11) has an elongated hole (111) extending along the height direction of the sleeve (11). The ultrasonic component (12) includes a guide portion located on the outer periphery of the piston portion (122). A guide protrusion (123) is formed on the guide portion. The guide protrusion (123) extends movably from the elongated hole (111) and can move along the elongated hole (111) under the action of an external driving force.

10. A nucleic acid detection analyzer, characterized in that, Includes the magnetic suction ultrasound device according to any one of claims 1 to 9.

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