Blood PCR (polymerase chain reaction) detection workstation

Abstract

The utility model discloses a blood PCR (Polymerase Chain Reaction) detection workstation, which relates to the technical field of molecular diagnosis and automatic fusion and comprises a casing, a microwell plate transfer mechanism, a PCR instrument, a film sealing instrument, an isolation door mechanism, a secondary positioning transfer table, an ultraviolet sterilizing mechanism, a moving mechanism and a material taking mechanism are arranged in the casing, the microwell plate transfer mechanism is fixedly connected with the casing, and the PCR instrument is fixedly connected with the casing. Through the combined arrangement of the microwell plate transfer mechanism, the PCR instrument, the film sealing instrument, the isolation door mechanism, the secondary positioning transfer table and the moving mechanism, the multi-process operation such as transfer, isolation, positioning, film sealing, PCR detection and data recording of a sample to be detected and sterilization and cleaning after an experiment can be automatically realized, and the whole PCR experiment process of the sample is completely free of manual interference. Therefore, the use threshold is greatly reduced, the traceability and accuracy of data are improved, the detection efficiency is improved, and meanwhile, the subsequent maintenance time and cost are greatly reduced.

Description

Technical Field

[0001] This utility model relates to the field of molecular diagnostics and automation integration technology, and in particular to a blood PCR testing workstation. Background Technology

[0002] PCR technology has wide applications in medicine, biology, and other fields. In medicine, it is used for pathogen detection and gene mutation analysis. In biological research, it is used for cloning DNA fragments and detecting gene expression levels. As a rapid, accurate, and efficient DNA amplification technique, PCR plays a vital role in biological research and experiments. Through PCR experiments, researchers can obtain large quantities of target DNA fragments, thereby advancing scientific research and medical advancements.

[0003] In existing technologies, the PCR experimental process typically includes nucleic acid extraction, regional isolation, isolation window transfer, sample sealing, position calibration, and PCR detection. This involves significant manual labor, and the sample analysis process often involves contact with volatile test solvents, posing safety and occupational safety risks. All operations are highly susceptible to human interference, and information management during sample transfer is mainly done manually. With a large number of samples, errors are prone to occur during transfer, leading to difficulties in traceability and uncontrollable data quality. Due to the inefficiency of manual operations and the inability of personnel to be on duty 24 hours a day, environmental monitoring laboratories can only process a limited number of samples per day, resulting in low detection efficiency. Utility Model Content

[0004] The purpose of this invention is to address the problems in existing technologies, such as the PCR experimental processing typically involving regional isolation, isolation window transfer, sample sealing, position calibration, and PCR detection, which require significant manual labor. Sample analysis often involves contact with volatile test solvents, viral samples, and nucleic acid substances, posing safety and occupational safety risks. All operations are highly susceptible to human interference. Information management during sample transfer is primarily manual, leading to errors in the transfer process when dealing with large numbers of samples, resulting in difficulties in traceability and uncontrollable data quality. Furthermore, due to the inefficiency of manual operation and the inability of personnel to maintain 24-hour monitoring, environmental monitoring laboratories can only process a limited number of samples daily, resulting in low detection efficiency. Therefore, this invention proposes a blood PCR testing workstation.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a blood PCR testing workstation, comprising a housing, wherein a microplate transfer mechanism, a PCR instrument, a sealing device, an isolation door mechanism, a secondary positioning transfer platform, a moving mechanism, and a material handling mechanism are installed inside the housing. The microplate transfer mechanism is fixedly connected to the housing, the sealing device is located below the microplate transfer mechanism, the isolation door mechanism is fixedly connected to the housing, the moving mechanism is fixedly installed at the bottom of the housing, the material handling mechanism is drivenly connected to the moving mechanism, and the secondary positioning transfer platform is located above the moving mechanism.

[0006] Preferably, the microplate transfer mechanism includes an X-axis moving frame, a Y-axis moving frame, and a Z-axis moving frame. The Y-axis moving frame is fixedly connected to the X-axis moving frame, and the Z-axis moving frame is fixedly connected to the Y-axis moving frame. A rotating electric gripper is fixedly connected to the lower end of the Z-axis moving frame, and an ultraviolet lamp is fixedly connected to one side of the X-axis moving frame.

[0007] Preferably, the isolation door mechanism includes a first plate, a second plate, and a mounting frame. The first plate and the mounting frame are both fixedly connected to the housing. Slide rails are fixedly connected to both sides of the middle part of one side of the mounting frame.

[0008] Preferably, a second plate is installed between the two slide rails, and an electric telescopic rod is installed between one side of the second plate and the first plate. The second plate is slidably connected to the slide rail.

[0009] Preferably, a transfer port is provided on one side of the middle part of the mounting bracket, and the transfer port is connected to a transfer slot on one side of the housing.

[0010] Preferably, the secondary positioning transfer platform includes a base, a support plate is fixedly connected to the upper end of the base, and a centering gripper is installed inside the base.

[0011] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0012] This invention, through the combined arrangement of a microplate transfer mechanism, a PCR instrument, a sealing device, an isolation gate mechanism, a secondary positioning transfer platform, and a moving mechanism, automatically performs multiple processes such as sample transfer, isolation, positioning, sealing, PCR testing, and data recording, achieving complete manual intervention throughout the entire PCR experiment. This significantly lowers the barrier to entry, improves data traceability and accuracy, increases testing efficiency, and substantially reduces subsequent maintenance time and costs. Attached Figure Description

[0013] Figure 1 A three-dimensional structural diagram of a blood PCR testing workstation is provided for this utility model;

[0014] Figure 2This invention provides a schematic diagram of the internal structure of a blood PCR testing workstation.

[0015] Figure 3 A three-dimensional structural schematic diagram of the microplate transfer mechanism in a blood PCR testing workstation is provided for this utility model;

[0016] Figure 4 A three-dimensional structural diagram of the isolation door mechanism in a blood PCR testing workstation is provided for this utility model;

[0017] Figure 5 This invention presents a three-dimensional structural diagram of a secondary positioning transfer station in a blood PCR testing workstation.

[0018] Legend: 1. Housing; 2. Microplate transfer mechanism; 21. X-axis moving frame; 22. Y-axis moving frame; 23. Z-axis moving frame; 24. UV lamp; 25. Rotary electric gripper; 3. PCR instrument; 4. Sealing device; 5. Isolation door mechanism; 51. Plate No. 1; 52. Plate No. 2; 53. Mounting frame; 54. Transfer port; 55. Slide rail; 56. Electric telescopic rod; 6. Secondary positioning transfer table; 61. Base; 62. Centering gripper; 63. Pallet; 7. Moving mechanism; 8. Material handling mechanism. Detailed Implementation

[0019] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0020] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0021] Example 1: As Figure 1 - Figure 5As shown, this utility model provides a blood PCR testing workstation, including a housing 1. Inside the housing 1 are installed a microplate transfer mechanism 2, a PCR instrument 3, a sealing device 4, an isolation door mechanism 5, a secondary positioning transfer platform 6, a moving mechanism 7, and a material handling mechanism 8. The microplate transfer mechanism 2 is fixedly connected to the housing 1. The sealing device 4 is located below the microplate transfer mechanism 2. The isolation door mechanism 5 is fixedly connected to the housing 1. The moving mechanism 7 is fixedly installed at the bottom of the housing 1. The material handling mechanism 8 is drivenly connected to the moving mechanism 7. The secondary positioning transfer platform 6 is located above the moving mechanism 7. The microplate transfer mechanism 2 includes an X-axis moving frame 21, a Y-axis moving frame 22, and a Z-axis moving frame 23. The Y-axis moving frame 22 is fixedly connected to the X-axis moving frame 21, and the Z-axis moving frame 23 is fixedly connected to the Y-axis moving frame 22. The lower end of the Z-axis moving frame 23 is fixedly connected to a rotating electric gripper 25. The side of the X-axis moving frame 21 is fixedly connected to an ultraviolet lamp 24. The isolation door mechanism 5 includes a first plate 51, a second plate 52 and a mounting frame 53. The first plate 51 and the mounting frame 53 are both fixedly connected to the housing 1. The middle of one side of the mounting frame 53 is fixedly connected to two slide rails 55. The second plate 52 is installed between the two slide rails 55. An electric telescopic rod 56 is installed between one side of the second plate 52 and the first plate 51. The second plate 52 is slidably connected to the slide rail 55. A transfer port 54 is opened on one side of the middle of the mounting frame 53. The transfer port 54 is connected to the transfer slot on one side of the housing 1. The secondary positioning transfer table 6 includes a base 61. The upper end of the base 61 is fixedly connected to a support plate 63. The centering gripper 62 is installed inside the base 61.

[0022] The specific setup and function of this embodiment are described below. Through the combined configuration of the microplate transfer mechanism 2, PCR instrument 3, sealing device 4, isolation gate mechanism 5, secondary positioning transfer platform 6, and moving mechanism 7, the system automatically performs multiple processes including sample transfer, isolation, positioning, sealing, PCR testing, data recording, and post-experiment disinfection and cleaning. This completely eliminates manual intervention throughout the entire PCR experiment. This significantly lowers the barrier to entry, improves data traceability and accuracy, increases testing efficiency, and greatly reduces subsequent maintenance time and costs.

[0023] The operating method and working principle of this device are as follows: During use, the electric telescopic rod 56 is retracted to open the transfer port 54. The moving mechanism 7 drives the material handling mechanism 8 to move out of the transfer port 54, placing the sample to be tested on the material handling mechanism 8. The material handling mechanism 8 then enters the housing 1, and the electric telescopic rod 56 extends to close the transfer port 54. The material handling mechanism 8 places the sample on the secondary positioning transfer table 6, where the centering gripper 62 clamps and positions the sample. The material handling mechanism 8 then moves the sample to the sealing device 4 for automatic sealing. The sealed sample is then moved by the material handling mechanism 8 to the secondary positioning transfer table 6 for positioning. The microplate transfer mechanism 2 then places the sample into the PCR instrument 3. After the PCR instrument 3 completes the test, it uploads the data to the central database. The material handling mechanism 8 then removes the tested sample from the transfer port 54. The sample can then be retrieved. After the experiment, the UV lamp 24 can be switched on and off periodically to disinfect and sterilize the workstation.

[0024] The above are merely preferred embodiments of this utility model and are not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from the technical solution of this utility model shall still fall within the protection scope of this utility model.

Claims

1. A blood PCR testing workstation, comprising a casing (1), characterized in that: The machine housing (1) is equipped with a microplate transfer mechanism (2), a PCR instrument (3), a sealing device (4), an isolation door mechanism (5), a secondary positioning transfer station (6), a moving mechanism (7), and a material handling mechanism (8). The microplate transfer mechanism (2) is fixedly connected to the machine housing (1). The sealing device (4) is located below the microplate transfer mechanism (2). The isolation door mechanism (5) is fixedly connected to the machine housing (1). The moving mechanism (7) is fixedly installed at the bottom of the machine housing (1). The material handling mechanism (8) is connected to the moving mechanism (7) by transmission. The secondary positioning transfer station (6) is located above the moving mechanism (7).

2. The blood PCR testing workstation according to claim 1, characterized in that: The microplate transfer mechanism (2) includes an X-axis moving frame (21), a Y-axis moving frame (22) and a Z-axis moving frame (23). The Y-axis moving frame (22) is fixedly connected to the X-axis moving frame (21), and the Z-axis moving frame (23) is fixedly connected to the Y-axis moving frame (22). A rotating electric gripper (25) is fixedly connected to the lower end of the Z-axis moving frame (23), and an ultraviolet lamp (24) is fixedly connected to one side of the X-axis moving frame (21).

3. The blood PCR testing workstation according to claim 1, characterized in that: The isolation door mechanism (5) includes a first plate (51), a second plate (52) and a mounting bracket (53). The first plate (51) and the mounting bracket (53) are both fixedly connected to the housing (1). Slide rails (55) are fixedly connected to both sides of the middle part of one side of the mounting bracket (53).

4. The blood PCR testing workstation according to claim 3, characterized in that: A second plate (52) is installed between the two slide rails (55), and an electric telescopic rod (56) is installed between one side of the second plate (52) and the first plate (51). The second plate (52) is slidably connected to the slide rail (55).

5. The blood PCR testing workstation according to claim 3, characterized in that: The mounting bracket (53) has a transfer port (54) on one side of the middle section, and the transfer port (54) is connected to the transfer slot on one side of the housing (1).

6. The blood PCR testing workstation according to claim 1, characterized in that: The secondary positioning transfer station (6) includes a base (61), a support plate (63) is fixedly connected to the upper end of the base (61), and a centering gripper (62) is installed inside the base (61).

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