Cell recovery device

Abstract

The utility model discloses a cell resuscitation device which comprises a bottom plate, the top of the bottom plate is provided with a constant-temperature water bath kettle through supporting legs, the top of the bottom plate is further provided with a lifting mechanism, the lifting end of the lifting mechanism is connected with a circular plate through a top plate and a connecting plate, an annular plate is arranged below the circular plate, and a cryopreservation tube storage rack is inserted into the top of the circular plate. A rotating rod is arranged on the inner bottom wall of the constant-temperature water bath kettle, a first stirring rod is arranged on the surface of the rotating rod, an annular rotating plate is rotationally arranged at the top of the constant-temperature water bath kettle, a vertical rod is arranged at the bottom of the annular rotating plate, a second stirring rod is arranged on the outer surface of the vertical rod, and a first gear sleeves the outer side surface of the annular rotating plate; a driving linkage assembly is arranged on the outer side surface of the constant-temperature water bath kettle; according to the device, warm water in the constant-temperature water bath kettle can be uniformly stirred under the condition that the cryopreservation tube is not moved, the resuscitation effect is ensured, meanwhile, the situation that cells are outwards gathered under the action of centrifugal force due to rotation in a traditional device, and consequently cells on the outer side are extruded is avoided, and then the cell activity is improved.

Description

Technical Field

[0001] This utility model relates to the field of cell water bath resuscitation technology, and in particular to a cell resuscitation device. Background Technology

[0002] Cell resuscitation is the opposite of cell cryopreservation; it is the process of cells resuming growth. It involves thawing cells frozen in liquid nitrogen or at -70°C and then reculturing them. When the cells are brought back to room temperature, their morphology and structure remain normal, and biochemical reactions can be restored.

[0003] Current cell resuscitation methods often employ water bath resuscitation, where cryovials are removed from liquid nitrogen containers and directly immersed in 37°C warm water. However, this method has a drawback: the water temperature near the cryovial drops rapidly, slowing down thawing, while other areas remain warmer, resulting in wasted heat. Existing technologies sometimes use rotation of the cryovial to ensure uniform water temperature, which helps with even heating. However, rotation causes the resuscitated cells to be subjected to centrifugal force, leading to cell aggregation and compression, which in turn affects cell biological activity. Therefore, we have designed a cell resuscitation device to address these technical problems. Utility Model Content

[0004] The purpose of this invention is to provide a cell resuscitation device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A cell resuscitation device includes a base plate. A constant-temperature water bath, cylindrical in shape, is fixedly mounted on the top of the base plate via multiple legs. A lifting mechanism is also provided on the top of the base plate. A top plate is located at the lifting end of the lifting mechanism. A connecting plate is located at the bottom of the other end of the top plate. A circular plate is located at the bottom of the connecting plate. An annular plate is located directly below the circular plate. Connecting side plates are fixedly connected to the left and right edges between the circular plate and the annular plate. Multiple cryopreservation tube racks are fixedly inserted into the top of the circular plate, extending to the bottom of the annular plate. The constant-temperature water bath... A rotating rod is rotatably mounted at the center of the inner bottom wall of the pot. Multiple first stirring rods are symmetrically arranged on the outer surface of the rotating rod. An annular rotating plate is rotatably mounted on the top of the constant temperature water bath along its circumference. Multiple vertical rods are evenly arranged on the bottom of the annular rotating plate along its circumference. The other end of each vertical rod extends along the inner side wall of the constant temperature water bath to the inner bottom of the pot. Multiple second stirring rods are arranged on the outer surface of each vertical rod, away from the inner side wall of the constant temperature water bath. A first gear is fitted on the outer surface of the annular rotating plate. A drive linkage assembly is provided on the outer surface of the constant temperature water bath.

[0007] As a preferred embodiment of this utility model: the drive linkage assembly includes a drive motor fixedly mounted on the top of the base plate, the output shaft of the drive motor is connected to a rotating shaft, the other end of the rotating shaft passes through the constant temperature water bath and is connected to the bottom end of the rotating rod, a first pulley is sleeved on the outside of the rotating shaft, the first pulley is rotatably mounted on the bottom of the constant temperature water bath, a transmission vertical rod is provided on the side of the constant temperature water bath away from the lifting mechanism, the bottom end of the transmission vertical rod is rotatably connected to the top of the base plate, a second gear is provided at the top end of the transmission vertical rod, the second gear is meshed with the first gear, a second pulley is sleeved on the outside of the transmission vertical rod, the second pulley and the first pulley are connected by belt drive, a number of support plates are provided on the outer wall of the constant temperature water bath along the vertical direction, the transmission vertical rod passes through the support plates and is rotatably connected to the support plates.

[0008] As a further preferred embodiment of this utility model: the bottom of the annular rotating plate is uniformly provided with a plurality of balls along its circumference, and the top of the constant temperature water bath is provided with a track groove along its circumference for the balls to roll and embed.

[0009] As a further preferred embodiment of this utility model, the outer diameter of the annular plate is equal to the diameter of the circular plate.

[0010] As a further preferred embodiment of this utility model: multiple cryopreservation tube storage racks are evenly distributed along the circumferential direction of the top surface of the annular plate, and the cryopreservation tube storage racks are fixedly connected to the annular plate.

[0011] As a further preferred embodiment of this utility model: the annular plate is located directly above the constant temperature water bath.

[0012] As a further preferred embodiment of the present invention: the plurality of first stirring rods and the plurality of second stirring rods are evenly distributed along the vertical direction.

[0013] As a further preferred embodiment of this utility model, the inner diameter of the annular rotating plate is smaller than the inner diameter of the constant temperature water bath.

[0014] The beneficial effects of this utility model are as follows: When using this device, multiple cryovials are sequentially placed into the cryovial storage rack. Then, a lifting mechanism controls the multiple cryovials to descend into the warm water of a constant-temperature water bath until the first stirring rod is positioned inside the multiple cryovials, and the second stirring rod is positioned outside the multiple cryovials. Simultaneously, the drive motor is started, driving the rotating shaft and the first pulley to rotate together. The rotating shaft drives the rotating rod to rotate, which in turn drives the multiple first stirring rods to rotate. The first pulley drives the second pulley to rotate via a belt, which in turn drives the transmission vertical rod to rotate, which in turn drives the second gear to rotate. The meshing action drives the first gear to rotate, which in turn drives the annular rotating plate to rotate. This, in turn, drives multiple vertical rods to rotate along with the annular rotating plate, which in turn drives multiple second stirring rods to rotate along the circumference of the inner wall of the constant temperature water bath. The direction of rotation is opposite to that of the first stirring rod. Therefore, the warm water in the constant temperature water bath can be uniformly stirred evenly while the cryopreservation tube remains stationary. The high uniformity of stirring helps the cryopreservation tube to be heated evenly, ensuring the thawing effect. At the same time, it avoids the outward aggregation of cells under centrifugal force caused by rotation in traditional devices, which leads to the compression of outer cells and thus improves cell activity. Attached Figure Description

[0015] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the present invention in another working state;

[0018] Figure 3 for Figure 1 Enlarged view of point A in the middle.

[0019] Wherein: 1-base plate, 2-lifting mechanism, 3-connecting side plate, 4-circular plate, 5-top plate, 6-connecting plate, 7-freeze tube storage rack, 8-ring plate, 9-second pulley, 10-belt, 11-drive motor, 12-first pulley, 13-support leg, 14-support plate, 15-transmission vertical rod, 16-rotating rod, 17-vertical rod, 18-constant temperature water bath, 19-second stirring rod, 20-first stirring rod, 21-second gear, 22-first gear, 23-ring rotating plate, 24-ball bearing. Detailed Implementation

[0020] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, illustrating only the basic structure of the present invention, and therefore only show the components relevant to the present invention.

[0021] Please see Figure 1-3 In this embodiment of the present invention, a cell resuscitation device includes a base plate 1. A constant temperature water bath 18 is fixedly mounted on the top of the base plate 1 via multiple support legs 13. The constant temperature water bath 18 has a cylindrical structure. A lifting mechanism 2 is also provided on the top of the base plate 1. A top plate 5 is provided at the lifting end of the lifting mechanism 2. A connecting plate 6 is provided at the bottom of the other end of the top plate 5. A circular plate 4 is provided at the bottom of the connecting plate 6. An annular plate 8 is provided directly below the circular plate 4. The outer diameter of the annular plate 8 is equal to the diameter of the circular plate 4. Connecting side plates 3 are fixedly connected to the left and right edges between the circular plate 4 and the annular plate 8. Multiple cryopreservation tube storage racks 7 are fixedly inserted into the top of the circular plate 4. The bottom of the cryopreservation tube storage racks 7 extends to the bottom of the annular plate 8. The cryopreservation tube storage racks 7 are fixedly connected to the annular plate 8. The multiple cryopreservation tube storage racks 7 are evenly distributed along the circumference of the top surface of the annular plate 8. The annular plate 8 is located in the constant temperature water bath 18. At the top, a rotating rod 16 is rotatably mounted at the center of the inner bottom wall of the constant temperature water bath 18. Multiple first stirring rods 20 are symmetrically mounted on the outer surface of the rotating rod 16. The multiple first stirring rods 20 are evenly distributed in the vertical direction. An annular rotating plate 23 is rotatably mounted on the top of the constant temperature water bath 18 in the circumferential direction. The inner diameter of the annular rotating plate 23 is smaller than the inner diameter of the constant temperature water bath 18. Multiple vertical rods 17 are evenly mounted on the bottom of the annular rotating plate 23 in the circumferential direction. The other end of the vertical rod 17 extends along the inner side wall of the constant temperature water bath 18 to the inner bottom of the constant temperature water bath 18. Multiple second stirring rods 19 are mounted on the outer surface of the vertical rod 17 and away from the inner side wall of the constant temperature water bath 18. The multiple second stirring rods 19 are evenly distributed in the vertical direction. A first gear 22 is sleeved on the outer surface of the annular rotating plate 23. A drive linkage assembly is provided on the outer surface of the constant temperature water bath 18.

[0022] The drive linkage assembly includes a drive motor 11 fixedly mounted on the top of the base plate 1. The output shaft of the drive motor 11 is connected to a rotating shaft. The other end of the rotating shaft passes through the constant temperature water bath 18 and is connected to the bottom end of the rotating rod 16. A first pulley 12 is sleeved on the outside of the rotating shaft. The first pulley 12 is rotatably mounted on the bottom of the constant temperature water bath 18. A transmission vertical rod 15 is provided on the side of the constant temperature water bath 18 away from the lifting mechanism 2. The bottom end of the transmission vertical rod 15 is rotatably connected to the top of the base plate 1. A second gear 21 is provided at the top of the transmission vertical rod 15. The second gear 21 meshes with the first gear 22. A second pulley 9 is sleeved on the outside of the transmission vertical rod 15. The second pulley 9 and the first pulley 12 are connected by a belt 10. Several support plates 14 are provided on the outer wall of the constant temperature water bath 18 in the vertical direction. The transmission vertical rod 15 passes through the support plates 14 and is rotatably connected to the support plates 14.

[0023] The bottom of the annular rotating plate 23 is uniformly provided with multiple balls 24 along its circumference, and the top of the constant temperature water bath 18 is provided with a track groove for the balls 24 to roll and embed. This design can change sliding friction to rolling friction, reduce friction and reduce power consumption.

[0024] The lifting mechanism 2 can be one of an electric actuator, a hydraulic cylinder, or a motor ball screw module, or other related reasonable and feasible lifting mechanisms. This is prior art and not an innovation of this application, so it will not be described in detail and is not shown in the figure.

[0025] Specifically, in use, multiple cryovials are sequentially placed into the cryovial storage rack 7. Then, the lifting mechanism 2 lowers the cryovials into the warm water of the constant temperature water bath 18 until the first stirring rod 20 is positioned inside the cryovials and the second stirring rod 19 is positioned outside the cryovials. Figure 2 As shown; simultaneously, the drive motor 11 is started, and the drive motor 11 drives the rotating shaft to rotate together with the first pulley 12. The rotating shaft drives the rotating rod 16 to rotate, which in turn drives multiple first stirring rods 20 to rotate. The first pulley 12 drives the second pulley 9 to rotate through the belt 10, which in turn drives the transmission vertical rod 15 to rotate, which in turn drives the second gear 21 to rotate. Through meshing, the first gear 22 is driven to rotate, which in turn drives the annular rotating plate 23 to rotate, which in turn drives multiple vertical rods 17 to rotate with the rotation of the annular rotating plate 23, which in turn drives multiple second stirring rods 19 to rotate in the circumferential direction along the inner wall of the constant temperature water bath 18, and the direction of rotation is opposite to the direction of rotation of the first stirring rods 20. Therefore, the warm water in the constant temperature water bath 18 can be evenly stirred while the cryopreservation tube is stationary. The high uniformity of stirring helps the cryopreservation tube to be heated evenly, ensuring the thawing effect. At the same time, it avoids the cells from gathering outward under the action of centrifugal force due to rotation in traditional devices, which causes the outer cells to be squeezed, thereby improving cell activity.

[0026] The above are merely preferred embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this utility model, based on the technical solution and inventive concept of this utility model, should be included within the scope of protection of this utility model.

Claims

1. A cell resuscitation device, comprising a base plate (1); characterized in that: A constant temperature water bath (18) is fixedly installed on the top of the base plate (1) by multiple support legs (13). The constant temperature water bath (18) has a cylindrical structure. The top of the base plate (1) is also provided with a lifting mechanism (2). The lifting end of the lifting mechanism (2) is provided with a top plate (5). The bottom of the other end of the top plate (5) is provided with a connecting plate (6). The bottom of the connecting plate (6) is provided with a circular plate (4). The circular plate (4) is provided with an annular plate (8) directly below it. The left and right edges between the circular plate (4) and the annular plate (8) are fixedly connected with connecting side plates (3). Multiple cryopreservation tube storage racks (7) are fixedly inserted on the top of the circular plate (4). The bottom of the cryopreservation tube storage racks (7) extends to the bottom of the annular plate (8). The inner bottom of the constant temperature water bath (18) A rotating rod (16) is rotatably provided at the center of the wall. Multiple first stirring rods (20) are symmetrically provided on the outer surface of the rotating rod (16). An annular rotating plate (23) is rotatably provided at the top of the constant temperature water bath (18) along its circumference. Multiple vertical rods (17) are evenly provided at the bottom of the annular rotating plate (23) along its circumference. The other end of the vertical rod (17) extends along the inner wall of the constant temperature water bath (18) to the inner bottom of the constant temperature water bath (18). Multiple second stirring rods (19) are provided on the outer surface of the vertical rod (17) and on the side away from the inner wall of the constant temperature water bath (18). A first gear (22) is sleeved on the outer surface of the annular rotating plate (23). A drive linkage assembly is provided on the outer surface of the constant temperature water bath (18).

2. The cell resuscitation device according to claim 1, characterized in that: The drive linkage assembly includes a drive motor (11) fixedly mounted on the top of the base plate (1). The output shaft of the drive motor (11) is connected to a rotating shaft. The other end of the rotating shaft passes through the constant temperature water bath (18) and is connected to the bottom end of the rotating rod (16). A first pulley (12) is sleeved on the outside of the rotating shaft. The first pulley (12) is rotatably mounted on the bottom of the constant temperature water bath (18). A transmission vertical rod (15) is provided on the side of the constant temperature water bath (18) away from the lifting mechanism (2). The bottom end of the transmission vertical rod (15) is rotatably connected to the top of the base plate (1). The top end of the transmission vertical rod (15) is provided with a second gear (21), which meshes with the first gear (22). The transmission vertical rod (15) is sleeved with a second pulley (9), which is connected to the first pulley (12) by a belt (10). The outer wall of the constant temperature water bath (18) is provided with several support plates (14) along the vertical direction.

3. The cell resuscitation device according to claim 2, characterized in that: The bottom of the annular rotating plate (23) is uniformly provided with a plurality of balls (24) along its circumference, and the top of the constant temperature water bath (18) is provided with a track groove for the balls (24) to roll and embed.

4. The cell resuscitation device according to claim 3, characterized in that: The outer diameter of the annular plate (8) is equal to the diameter of the circular plate (4).

5. The cell resuscitation device according to claim 4, characterized in that: Multiple cryopreservation tube storage racks (7) are evenly distributed along the circumferential direction of the top surface of the annular plate (8), and the cryopreservation tube storage racks (7) are fixedly connected to the annular plate (8).

6. The cell resuscitation device according to claim 5, characterized in that: The annular plate (8) is located directly above the constant temperature water bath (18).

7. The cell resuscitation device according to claim 1, characterized in that: The plurality of first stirring rods (20) and the plurality of second stirring rods (19) are evenly distributed along the vertical direction.

8. The cell resuscitation device according to claim 7, characterized in that: The inner diameter of the annular rotating plate (23) is smaller than the inner diameter of the constant temperature water bath (18).

9. A cell resuscitation device according to claim 2, characterized in that: The transmission vertical rod (15) passes through the support plate (14) and is rotatably connected to the support plate (14).

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