Grading separation device for natto extract

Abstract

The natto extract grading separation device comprises a pulping incubator, a centrifugal separator and a membrane separation module which are sequentially connected, the membrane separation module comprises a first-stage separation membrane device, a second-stage separation membrane device and a third-stage separation membrane device which are sequentially connected, the centrifugal separator is communicated with the first-stage separation membrane device through a first-stage liquid storage tank, and the third-stage separation membrane device is communicated with the second-stage liquid storage tank through a second-stage liquid storage tank. And the first-stage separation membrane device is communicated with the second-stage separation membrane device through the second-stage liquid storage tank. The pulping incubator, the centrifugal separator and the membrane separation module which are connected in sequence are adopted, incubation of natto can be achieved through the pulping incubator, the peptide content is increased through active enzymolysis of nattokinase on soybean protein, solid-liquid separation is achieved through high-speed centrifugation of a filter screen in the centrifugal separator, natto residues and thalli are recycled, and the yield of natto is increased. Nattokinase and polypeptide components are recovered in a graded manner by combining a first-stage separation membrane device, a second-stage separation membrane device and a third-stage separation membrane device with three pore diameters, and continuous production and separation can be realized.

Description

Technical Field

[0001] This utility model relates to the field of natto extract separation technology, and more specifically, to a natto extract grading and separation device. Background Technology

[0002] Natto is a fermentation product made from cooked soybeans fermented with Bacillus subtilis. Studies have found that its active ingredients include Bacillus subtilis, nattokinase, and short peptides of varying molecular weights. Peptides with a molecular weight less than 2000 Da have lipid-lowering effects, peptides with a molecular weight of 2000-10000 Da have blood pressure-lowering effects, while nattokinase has a molecular weight of 27000 Da.

[0003] Based on market demand, it is necessary to extract and utilize Bacillus subtilis, nattokinase, and peptides separately.

[0004] However, in actual production, different production processes need to be designed for different extracts, resulting in complicated process steps, time and labor consumption, and excessive production costs. Moreover, when centrifuging, traditional centrifugation has poor solid-liquid separation effect, which affects its purification efficiency and effect.

[0005] Therefore, it is necessary to propose a graded separation device for natto extract to achieve graded extraction of Bacillus subtilis, nattokinase and short peptides of different molecular weights, which is of great significance for realizing continuous and automated production. Utility Model Content

[0006] This invention provides a grading and separation device for natto extract. The grading and separation device for natto extract combines pulping and incubation, centrifugation and membrane separation to solve the problems of complex extraction processes of Bacillus subtilis, nattokinase and peptides from natto, which cannot be automated and have low separation efficiency.

[0007] According to one aspect of the present invention, a natto extract grading and separation device is provided, comprising a pulping incubator, a centrifuge, and a membrane separation module connected in sequence. The membrane separation module includes a primary membrane separator, a secondary membrane separator, and a tertiary membrane separator connected in sequence. The centrifuge is connected to the primary membrane separator via a primary storage tank. The primary membrane separator is connected to the secondary membrane separator via a secondary storage tank. The tertiary membrane separator is connected to the tertiary membrane separator via a tertiary storage tank.

[0008] Based on the above scheme, the preferred embodiment is that the pulping incubator includes a stirrer with a stirring paddle, the stirrer is provided with a feed inlet, a water inlet and a discharge outlet, and an electric heating controller is provided inside the stirrer.

[0009] Based on the above-mentioned scheme, preferably, the centrifugal separator includes a support plate, a shell, a stirring shaft, and a filter screen. The support plate is equipped with a motor connected to the stirring shaft. The stirring shaft is hollow and connected to the output end of the pulping incubator. A feed hole is provided in the middle of the stirring shaft. The filter screen is fitted on the stirring shaft. The shell is fitted on the outside of the filter screen to form a filtration chamber. The support plate is provided with a liquid outlet pipe communicating with the filtration chamber. The filter screen and the support plate form a slag cavity. The support plate is provided with a discharge port communicating with the slag cavity.

[0010] Based on the above scheme, a preferred embodiment is that a support shell is also fitted on the stirring shaft, and the support shell, the filter screen and the support plate form the slag cavity.

[0011] Based on the above scheme, preferably, the support shell, filter screen and outer shell are in the shape of a cone.

[0012] Preferably, based on the above scheme, the first-stage separation membrane device is provided with a first-stage separation membrane with a pore size of 10000 Da; the second-stage separation membrane device is provided with a second-stage separation membrane with a pore size of 2000 Da; and the third-stage separation membrane device is provided with a third-stage separation membrane with a pore size of 100 Da.

[0013] This invention relates to a natto extract grading and separation device, which employs a pulping incubator, a centrifuge, and a membrane separation module connected in sequence. The pulping incubator can incubate natto, and the active enzymes of nattokinase can be used to hydrolyze soybean protein to increase peptide content. Then, in the centrifuge, high-speed centrifugation through a filter screen achieves solid-liquid separation, recovering natto residue and microorganisms. Combined with the graded recovery of nattokinase and peptide components through a primary, secondary, and tertiary separation membrane with three different pore sizes, continuous production separation can be achieved. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. In the drawings:

[0015] Figure 1 This is a schematic diagram of the principle structure of the natto extract fractionation and separation device of this utility model.

[0016] Figure 2 This is a structural diagram of the centrifugal separator of this utility model;

[0017] Figure 3This is a structural diagram of the filter screen of this utility model;

[0018] Explanation of icon numbers:

[0019] 1. Pulping incubator; 11. Agitator; 12. Agitator paddle; 13. Feed inlet; 14. Water inlet; 15. Discharge outlet; 16. Electric heating controller;

[0020] Centrifugal separator 2, support plate 21, outer shell 22, stirring shaft 23, filter screen 24, motor 25, feed hole 26, filter chamber 27, liquid outlet pipe 28, slag chamber 29, discharge port 291, support shell 292;

[0021] Membrane separation module 3, primary separation membrane 31, primary storage tank 32, primary separation membrane 33, secondary separation membrane 34, secondary storage tank 35, secondary separation membrane 36, tertiary separation membrane 37, tertiary storage tank 38, tertiary separation membrane 39. Detailed Implementation

[0022] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0023] It should be understood that, when used in this specification and the appended claims, the term "comprising" indicates the presence of a descriptive feature, integral, step, operation, element, and / or component, but does not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components, and / or sets.

[0024] To keep the drawings concise, only the parts relevant to this invention are shown schematically in each figure, and they do not represent the actual structure of the product. Furthermore, for ease of understanding, in some figures, only one of the components with the same structure or function is schematically depicted, or only one is labeled. In this document, "one" not only means "only one," but can also mean "more than one."

[0025] It should also be further understood that the term “and / or” as used in this application specification and the appended claims means any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0026] In the embodiments shown in the accompanying drawings, the directional indications (such as up, down, left, right, front, and back) used to explain the structure and movement of the various components of this invention are relative rather than absolute. These descriptions are appropriate when these components are in the positions shown in the drawings. If the descriptions of the positions of these components change, these directional indications also change accordingly.

[0027] Furthermore, in the description of this application, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the specific implementation methods of this utility model will be described below with reference to the accompanying drawings. Obviously, the drawings described below are merely some embodiments of this utility model. For those skilled in the art, other drawings and other implementation methods can be obtained based on these drawings without any creative effort.

[0029] Please see Figure 1 and combined Figure 2 and Figure 3 As shown, the present invention discloses a natto extract grading and separation device, comprising a pulping incubator 1, a centrifuge 2, and a membrane separation module 3 connected in sequence. The membrane separation module 3 comprises a primary membrane separator 31, a secondary membrane separator 34, and a tertiary membrane separator 37 connected in sequence. The centrifuge 2 is connected to the primary membrane separator 31 via a primary storage tank 32. The primary membrane separator 31 is connected to the secondary membrane separator 34 via a secondary storage tank 35. The tertiary membrane separator 37 is connected to the tertiary membrane separator 37 via a tertiary storage tank 38.

[0030] Specifically, the pulping incubator 1 of this utility model includes a stirrer 11 with a stirring paddle 12. The stirrer 11 is provided with a feed inlet 13, a water inlet 14 and a discharge outlet 15, and an electric heating controller 16 is provided inside the stirrer 11.

[0031] In use, fermented natto and purified water are added to the mixer 11 in a certain ratio. The mixer 12 is then stirred to obtain a uniform slurry by controlling its speed and time. The temperature control switch is turned on, and the temperature is controlled for 1-2 hours via the electric heating controller 16 to allow the nattokinase and soybean protein to be fully enzymatically hydrolyzed, thereby increasing the peptide content in the slurry.

[0032] After incubation, the slurry enters centrifuge 2 via a pressurized pump, where solid-liquid separation is achieved through high-speed centrifugation. The solids in centrifuge 2 contain Bacillus subtilis and natto residue, and Bacillus subtilis is recovered. The supernatant flows through the outlet of centrifuge 2 into membrane separation module 3 for separation of different molecular weights, thereby achieving automated, step-by-step separation and continuous automated production.

[0033] In order to improve the separation effect and avoid the solid containing too much supernatant during solid-liquid separation, the centrifuge 2 of this utility model includes a support plate 21, a shell 22, a stirring shaft 23 and a filter screen 24. The support plate 21 is provided with a discharge port 291 for discharging Bacillus subtilis and natto residue and a liquid outlet pipe 28 for discharging supernatant.

[0034] like Figure 2 As shown, a motor 25 is mounted on the support plate 21 of this utility model. The output end of the motor 25 is connected to a hollow stirring shaft 23. The stirring shaft 23 is provided with a feed hole 26, and a liquid outlet pipe 28 is connected to the other end of the stirring shaft 23. The stirring shaft 23 is fitted with a support shell 292, a filter screen 24, and an outer shell 22. The support shell 292 rotates together with the stirring shaft 23, and its surface is closed. A cavity is formed between the support shell 292 and the filter screen 24, which is connected to the feed hole 26 and is used to contain the supernatant introduced by the feed hole 26. The filter screen 24 and the support plate 21 form a slag cavity 29. The support plate 21 is provided with a discharge port 291 that is connected to the slag cavity 29. The outer shell 22 is fitted over the filter screen 24 to form a filter cavity 27. The support plate 21 is provided with a liquid outlet pipe 28 that is connected to the filter cavity 27.

[0035] After incubation, the slurry is introduced into the stirring shaft 23 by a pressure pump and enters the cavity through the feed hole 26. The motor 25 drives the stirring shaft 23 to rotate, so that the slurry is subjected to centrifugal force, which disperses it towards the filter screen 24 and impacts the inner surface of the filter screen 24, thus throwing out the supernatant in the slurry. This achieves solid-liquid separation and avoids excessive supernatant content in the natto residue, which would affect its purification efficiency and utilization rate.

[0036] As a preferred embodiment of the present invention, the support shell 292, filter screen 24 and outer shell 22 of the present invention are conical in shape, and the inclination angle of the cone is 60° to 85°. If the inclination angle is less than 60°, the slurry will not rise along the inclined surface but will fall directly to the lower side under the action of centrifugal force, affecting the dehydration rate of the supernatant.

[0037] The filter 24 of this utility model includes an inner mesh 242 and an outer mesh 241 that are sequentially arranged. The inner mesh 242 is provided with tapered filter holes 243, and the outer mesh 241 is provided with through holes 244. The diameter of the through holes 244 on the outer mesh 241 is larger than the diameter of the filter holes 243 on the inner mesh 242.

[0038] This invention, through the use of conical filter holes 243, allows solid particles in the slurry to remain on the inclined surface of the inner mesh 242 for an extended period, thereby increasing the dehydration time and ensuring thorough dehydration.

[0039] Furthermore, it should be noted that the first-stage membrane separator 31 of this utility model is provided with a first-stage membrane 33, and the membrane pore size of the first-stage membrane 33 is 10000 Da; the second-stage membrane separator 34 is provided with a second-stage membrane 36, and the membrane pore size of the second-stage membrane 36 is 2000 Da; the third-stage membrane separator 37 is provided with a third-stage membrane 39, and the membrane pore size of the third-stage membrane 39 is 100 Da.

[0040] When the supernatant flows into the primary storage tank 32 through the outlet pipe 28 of the centrifuge 2, the primary storage tank 32 contains nattokinase and peptides of varying amounts. It is then pumped into the primary separation membrane 33 within the primary separation membrane unit 31. The pore size of the primary separation membrane 33 is 10000 Da. The pump facilitates the circulation and separation of the membrane and the permeate. The permeate flows through the membrane outlet into the secondary storage tank 35, while the concentrate flows back into the primary storage tank 32. After separation by the primary separation membrane 33, the nattokinase component with a pore size greater than 10000 Da is stored in the primary storage tank 32, while the peptide component with a pore size less than 10000 Da flows through the membrane outlet into the secondary storage tank 35.

[0041] Peptide components smaller than 10000 Da enter the secondary storage tank 35 through the separation membrane outlet. The secondary separation membrane 36 within the secondary separation membrane unit 34 has a pore size of 2000 Da, and step 3 is repeated. After separation by the secondary separation membrane 36, peptide components between 2000-10000 Da are stored in the secondary storage tank 35. Peptide components smaller than 2000 Da enter the tertiary storage tank 38 through the separation membrane outlet.

[0042] Peptide components smaller than 2000 Da enter the tertiary storage tank 38 through the separation membrane outlet. The tertiary separation membrane 39 has a pore size of 100 Da, and step 4 is repeated. After separation by the tertiary separation membrane 39, peptide components between 100-2000 Da are stored in the tertiary storage tank 38. Components smaller than 100 Da are discharged through the separation membrane outlet. Components smaller than 100 Da contain virtually no other substances besides water, allowing for recycling and avoiding wastewater discharge.

[0043] Through the above steps, the recovery of four natto extracts was finally achieved, including Bacillus subtilis component, nattokinase component with a value greater than 10,000 Da, polypeptide component with a value between 2,000 and 10,000 Da, and polypeptide component with a value between 100 and 2,000 Da.

[0044] Finally, the method described in this application is merely a preferred embodiment and is not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the scope of protection of this utility model.

Claims

1. A natto extract fractionation and separation device, characterized in that, The device includes a pulping incubator, a centrifuge, and a membrane separation module connected in sequence. The membrane separation module includes a primary membrane separator, a secondary membrane separator, and a tertiary membrane separator connected in sequence. The centrifuge is connected to the primary membrane separator via a primary storage tank. The primary membrane separator is connected to the secondary membrane separator via a secondary storage tank. The tertiary membrane separator is connected to the tertiary membrane separator via a tertiary storage tank.

2. The natto extract fractionation and separation device as described in claim 1, characterized in that, The pulping incubator includes a stirrer with a stirring paddle, the stirrer being provided with a feed inlet, a water inlet and a discharge outlet, and an electric heating controller being provided inside the stirrer.

3. The natto extract fractionation and separation device as described in claim 1, characterized in that, The centrifuge includes a support plate, a shell, a stirring shaft, and a filter screen. A motor connected to the stirring shaft is mounted on the support plate. The stirring shaft is hollow and connected to the output end of the pulping incubator. A feed hole is provided in the middle of the stirring shaft. The filter screen is fitted onto the stirring shaft. The shell is fitted onto the filter screen to form a filtration chamber. A liquid outlet pipe communicating with the filtration chamber is provided on the support plate. The filter screen and the support plate form a slag cavity. A discharge port communicating with the slag cavity is provided on the support plate.

4. The natto extract fractionation and separation device as described in claim 3, characterized in that, A support shell is also fitted onto the stirring shaft, and the support shell, together with the filter screen and the support plate, forms the slag cavity.

5. The natto extract fractionation and separation device as described in claim 4, characterized in that, The support shell, filter screen, and outer shell are conical in shape.

6. The natto extract grading and separation device as described in claim 4, characterized in that, The first-stage membrane separator contains a first-stage membrane with a pore size of 10000 Da; the second-stage membrane separator contains a second-stage membrane with a pore size of 2000 Da; and the third-stage membrane separator contains a third-stage membrane with a pore size of 100 Da.

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