Purification device for nucleic acid extraction

Abstract

The utility model discloses a purification device for nucleic acid extraction, and relates to the field of nucleic acid extraction, the purification device comprises a device main body and a rotary table, the top of the rotary table is provided with a clamping assembly, and the clamping assembly comprises a plurality of placing cylinders arranged at the top of the rotary table. Through the arrangement of the clamping assembly, after a plurality of test tubes are respectively placed in the placing cylinder, a worker can rotate a threaded sleeve, so that the threaded sleeve moves downwards and drives a movable sleeve to move downwards, then a connecting plate and a movable ring are driven to move downwards, when the movable ring moves downwards, a pressing plate can be pushed to rotate, and the pressing plate pushes a sliding seat to move; when the silica gel pads on the clamping plates are tightly attached to the test tubes, the threaded sleeves are not rotated any more, the clamping plates and the silica gel pads can clamp and fix the test tubes at the moment, follow-up rotation is facilitated, the multiple test tubes can be clamped and fixed at the same time in the mode, and therefore the clamping and fixing steps of the test tubes are simplified; and the clamping and fixing efficiency of the test tube is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of nucleic acid extraction, and specifically to a purification device for nucleic acid extraction. BACKGROUND

[0002] Nucleic acid is a biological macromolecular compound polymerized by many nucleotides, which is not only an indispensable substance for protein biosynthesis, but also a material basis for biological inheritance. Nucleic acid has a large relative molecular mass and widely exists in all animal and plant cells and microorganisms. Nucleic acid in a biological body is often combined with protein to form nucleoprotein. Different nucleic acids have different chemical compositions and nucleotide arrangement sequences. Nucleic acid is a carrier of genetic information and a material basis for gene expression. Whether nucleic acid structure or function is studied, nucleic acid needs to be separated and purified first. The quality of a nucleic acid sample will directly affect the success of an experiment.

[0003] A purification device for nucleic acid extraction is disclosed in Chinese Patent No. CN220579295U. The utility model is fixed by setting a fixing assembly, so that the first fixing ring and the second fixing ring are connected through the cooperation of the clamping plate and the limiting rod, thereby fixing the test tube. The test tube is protected by the rubber pad.

[0004] According to the above-mentioned patent content, the fixing assembly is provided to fix the test tube, which is convenient for subsequent rotation of the test tube. However, since multiple test tubes need to be placed, multiple fixing assemblies are required to fix multiple test tubes. When fixing the test tube, it needs to be inserted into the fixing assembly one by one, which consumes a lot of time and is tedious to operate, thereby reducing the fixing efficiency of multiple test tubes. UTILITY MODEL CONTENT

[0005] Therefore, the utility model aims to provide a purification device for nucleic acid extraction to solve the technical problem of low clamping and fixing efficiency of multiple test tubes.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a purification device for nucleic acid extraction, comprising a device main body and a turntable, a clamping assembly is arranged on the top of the turntable, and the clamping assembly comprises multiple placing barrels mounted on the top of the turntable and a threaded column mounted at the middle position of the top of the turntable, a movable sleeve and a threaded sleeve are respectively sleeved on the outer wall of the threaded column, a movable groove is formed in the top of the movable sleeve, and multiple movable blocks are arranged in the movable groove, a movable ring is sleeved on the outer wall of each placing barrel, and a connecting plate is connected between the movable ring and the movable sleeve, two sliding rods are arranged on the outer wall of the placing barrel, a sliding seat is sleeved on the outer wall of the sliding rod, a connecting rod penetrating into the inside of the placing barrel is mounted on the sliding seat, and a pressing plate is rotatably connected between the sliding seat and the movable ring, a clamping plate is mounted on the end of the connecting rod, and a silica gel pad is arranged on one side of the clamping plate.

[0007] By adopting the above technical scheme, when the movable ring moves downward, the pressing plate is pushed to rotate, the sliding seat is pushed to move, and the connecting rod and the clamping plate are driven to move, thereby facilitating the adjustment of the position of the clamping plate.

[0008] Further, the outer wall of the placing cylinder is provided with two limiting grooves, the limiting grooves are provided with limiting blocks inside, and the limiting blocks are connected with the inner wall of the movable ring.

[0009] By adopting the above technical scheme, when the movable ring moves, the limiting block slides in the limiting groove, thereby improving the stability when the movable ring moves.

[0010] Further, the top of the movable block is connected with the bottom of the threaded sleeve, the movable groove is annular, and the movable block is slidingly connected with the movable groove.

[0011] By adopting the above technical scheme, when the threaded sleeve rotates, the movable block slides in the movable groove, so that the threaded sleeve drives the movable sleeve to move when moving.

[0012] Further, the test tube is placed in the placing cylinder, and the top of the device body is rotatably connected with a sealing cover.

[0013] By adopting the above technical scheme, the placing cylinder is provided to facilitate the placement of the test tube, and after the test tube is inserted into the placing cylinder, the sealing cover can be covered on the device body.

[0014] Further, an electric heating rod is installed on one side of the inside of the device body, and a temperature sensor is installed on the other side of the inside of the device body.

[0015] By adopting the above technical scheme, the electric heating rod can heat the test tube, and the temperature sensor can detect the temperature inside the device body.

[0016] Further, a rotating shaft is connected to the lower part of the inside of the device body through a bearing, and a turntable is installed at the top end of the rotating shaft.

[0017] By adopting the above technical scheme, the rotating shaft is provided to facilitate the rotation of the turntable, and when the turntable rotates, it drives the placing cylinder to rotate and drives the test tube to rotate, so as to play a centrifugal role.

[0018] Further, a motor is also installed on the lower part of the inside of the device body, and a gear is connected to the output end of the motor.

[0019] By adopting the above technical scheme, when the turntable needs to be rotated, the staff can start the motor, and the motor can drive the gear to rotate.

[0020] Further, a gear ring is installed on the outer wall of the turntable, and the gear is engaged with the gear ring.

[0021] By adopting the technical scheme, the gear rotates to drive the gear ring to rotate, and the gear ring rotates to drive the rotating table to rotate.

[0022] Further, the movable sleeve is in sliding connection with the threaded column, and the outer wall of the threaded sleeve is provided with anti-skid lines.

[0023] By adopting the technical scheme, the movable sleeve can move on the threaded column, so that the position of the movable sleeve can be adjusted.

[0024] Further, the clamping plate and the silica gel pad are both in arc shape, and the clamping plate and the connecting rod are both in sliding connection with the sliding seat and the sliding rod.

[0025] By adopting the technical scheme, the sliding seat slides on the sliding rod to drive the connecting rod and the clamping plate to move, so that the position of the clamping plate can be adjusted.

[0026] In summary, the utility model mainly has the following beneficial effects:

[0027] 1. The utility model discloses a clamping assembly, which is arranged on the placing cylinder, and is used for clamping and fixing the test tubes.

[0028] 2. The utility model discloses a movable block and a movable groove, which are arranged on the threaded sleeve. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is a whole three-dimensional structure schematic diagram of the utility model;

[0030] Figure 2 It is a whole cross-sectional structure schematic diagram of the utility model;

[0031] Figure 3 It is a placing cylinder structure schematic diagram of the utility model;

[0032] Figure 4 It is a movable ring structure schematic diagram of the utility model;

[0033] Figure 5 It is the schematic view of the clamping plate structure of the utility model;

[0034] Figure 6 It is the schematic view of the Figure 2 Enlarged structure schematic view of A place in the middle.

[0035] In the figure: 1, device main body; 2, sealing cover; 3, electric heating rod; 4, temperature sensor; 5, control panel; 6, motor; 7, gear; 8, clamping assembly; 801, placing cylinder; 802, threaded column; 803, threaded sleeve; 804, movable block; 805, movable slot; 806, movable sleeve; 807, connecting plate; 808, movable ring; 809, pressing plate; 810, sliding seat; 811, sliding rod; 812, connecting rod; 813, clamping plate; 814, silica gel pad; 815, limiting groove; 816, limiting block; 9, rotary table; 10, gear ring; 11, rotating shaft. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. The embodiments described below with reference to the drawings are exemplary and are used only for explaining the utility model and cannot be understood as limiting the utility model.

[0037] The embodiments of the utility model will be described below according to the overall structure of the utility model.

[0038] Embodiment one:

[0039] A kind of purification device for nucleic acid extraction, as shown in Figures 1-6 It includes device main body 1 and rotary table 9, the top of rotary table 9 is equipped with clamping assembly 8, the inside one side of device main body 1 is installed with electric heating rod 3, the inside other side of device main body 1 is installed with temperature sensor 4, electric heating rod 3 can heat test tube, and temperature sensor 4 can detect the temperature in the inside of device main body 1, clamping assembly 8 includes multiple placing cylinders 801 installed in the top of rotary table 9 and threaded column 802 installed in the middle position of the top of rotary table 9, the outer wall of threaded column 802 is respectively sleeved with movable sleeve 806 and threaded sleeve 803, the top of movable sleeve 806 is equipped with movable slot 805, and the inside of movable slot 805 is provided with multiple movable blocks 804, the outer wall of multiple placing cylinders 801 is sleeved with movable ring 808, and connecting plate 807 is connected between movable ring 808 and movable sleeve 806, the outer wall of placing cylinder 801 is equipped with two sliding rods 811, the outer wall of sliding rod 811 is sleeved with sliding seat 810, and sliding seat 810 is installed with connecting rod 812 penetrating into the inside of placing cylinder 801, and pressing plate 809 is rotatably connected between sliding seat 810 and movable ring 808.

[0040] Specific, the end of the connecting rod 812 is provided with a clamping plate 813, and one side of the clamping plate 813 is provided with a silica gel pad 814. When the movable ring 808 moves downward, the movable ring 808 can push the pressing plate 809 to rotate, the pressing plate 809 pushes the sliding seat 810 to move, and then the connecting rod 812 and the clamping plate 813 are driven to move, so that the position of the clamping plate 813 is adjusted conveniently. The top of the movable block 804 is connected with the bottom of the threaded sleeve 803, the movable groove 805 is annular, the movable block 804 is slidably connected with the movable groove 805, when the threaded sleeve 803 rotates, the threaded sleeve 803 drives the movable block 804 to slide in the movable groove 805, so that when the threaded sleeve 803 moves, the movable sleeve 806 is driven to move, the movable sleeve 806 is slidably connected with the threaded column 802, and the outer wall of the threaded sleeve 803 is provided with anti-skid lines, so that the movable sleeve 806 can move on the threaded column 802, and thus the position of the movable sleeve 806 can be adjusted.

[0041] Specific, the clamping plate 813 and the silica gel pad 814 are both arc-shaped, the clamping plate 813 and the connecting rod 812 are both slidably connected with the sliding rod 811 through the sliding seat 810, so that the sliding seat 810 slides on the sliding rod 811 and drives the connecting rod 812 and the clamping plate 813 to move, and thus the position of the clamping plate 813 can be adjusted. The test tube is placed in the placing cylinder 801, the top of the device main body 1 is rotatably connected with the sealing cover 2, and the placing cylinder 801 is convenient for placing the test tube. After the test tube is inserted into the placing cylinder 801, the sealing cover 2 can be placed on the device main body 1, and when the turntable 9 rotates, the placing cylinder 801 is driven to rotate and the test tube is driven to rotate, so that the centrifugal effect is achieved.

[0042] Embodiment two:

[0043] On the basis of the above-mentioned embodiment one, in order to facilitate the rotation of the test tube in the placing cylinder 801, the following structure is arranged.

[0044] Referring to Figures 1-4 The inside of the device main body 1 is connected with a rotating shaft 11 through a bearing at the bottom, the top of the rotating shaft 11 is provided with a turntable 9, the inside of the device main body 1 is also provided with a motor 6 at the bottom, the output end of the motor 6 is connected with a gear 7, when the turntable 9 needs to be rotated, the staff can start the motor 6, the motor 6 works to drive the gear 7 to rotate, the outer wall of the turntable 9 is provided with a gear ring 10, the gear 7 is engaged with the gear ring 10, when the gear 7 rotates, the gear ring 10 is driven to rotate, when the gear ring 10 rotates, the turntable 9 is driven to rotate, and the placing cylinder 801 and the test tube are driven to rotate, the outer surface of the device main body 1 is provided with a control panel 5, the control panel 5 is electrically connected with the motor 6, the electric heating rod 3 and the temperature sensor 4 respectively, so that the motor 6, the electric heating rod 3 and the temperature sensor 4 can be started or stopped through the control panel 5.

[0045] Embodiment three:

[0046] On the basis of the above embodiment one, in order to improve the stability when the movable ring 808 moves, the following structure is set.

[0047] Specifically, the outer wall of the placing cylinder 801 is provided with two limiting grooves 815, the inside of the limiting groove 815 is provided with a limiting block 816, and the limiting block 816 is connected with the inner wall of the movable ring 808, when the movable ring 808 moves, it will drive the limiting block 816 to slide in the limiting groove 815, thereby improving the stability when the movable ring 808 moves.

[0048] The working principle of the utility model is: first, before using the device, the power supply can be connected, then the sealing cover 2 is opened, then a plurality of test tubes are placed in the placing cylinder 801, the staff can rotate the threaded sleeve 803, so that the threaded sleeve 803 moves down and drives the movable sleeve 806 to move down, then drives the connecting plate 807 and the movable ring 808 to move down, when the movable ring 808 moves down, it can push the pressing plate 809 to rotate, the pressing plate 809 pushes the sliding seat 810 to move, then drives the connecting rod 812 and the clamping plate 813 to move, when the silica gel pad 814 on the clamping plate 813 is tightly attached to the test tube, the threaded sleeve 803 is not rotated, at this time, the clamping plate 813 and the silica gel pad 814 can clamp and fix the test tube, then the sealing cover 2 is covered.

[0049] Then start the electric heating rod 3 and the motor 6, the motor 6 works to drive the gear 7 to rotate, then drives the gear ring 10 to rotate, then drives the rotating table 9 to rotate, and drives the placing cylinder 801 to rotate, then drives the test tube to rotate, at the same time, the electric heating rod 3 can heat the test tube, so as to carry out passivation treatment on the nucleic acid in the test tube, and the temperature sensor 4 can detect the temperature in the device main body 1.

[0050] Although the embodiments of the utility model have been shown and described, the specific embodiments are only an explanation of the utility model, and are not a limitation of the utility model, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable way, and the person skilled in the art can make non-creative modification, replacement and change of the embodiments according to the needs after reading the specification without departing from the principles and purposes of the utility model, but as long as it is within the scope of the claims of the utility model, it is protected by the patent law.

Claims

1. A purification device for nucleic acid extraction comprising a device body (1) and a turntable (9), characterized in that: The top of the rotating platform (9) is provided with a clamping assembly (8), and the clamping assembly (8) comprises a plurality of placing barrels (801) mounted on the top of the rotating platform (9) and a threaded column (802) mounted at the middle position of the top of the rotating platform (9), the outer wall of the threaded column (802) is respectively sleeved with a movable sleeve (806) and a threaded sleeve (803), the top of the movable sleeve (806) is provided with a movable groove (805), and the inside of the movable groove (805) is provided with a plurality of movable blocks (804), the outer wall of each of the plurality of placing barrels (801) is sleeved with a movable ring (808), and the movable ring (808) and the movable sleeve (806) are connected through a connecting plate (807), the outer wall of the placing barrel (801) is provided with two sliding rods (811), the outer wall of the sliding rod (811) is sleeved with a sliding seat (810), a connecting rod (812) penetrating into the inside of the placing barrel (801) is mounted on the sliding seat (810), and the sliding seat (810) and the movable ring (808) are rotatably connected through a pressing plate (809), the end of the connecting rod (812) is provided with a clamping plate (813), and one side of the clamping plate (813) is provided with a silica gel pad (814).

2. The purification device for nucleic acid extraction according to claim 1, characterized by: The outer wall of the placing barrel (801) is provided with two limiting grooves (815), and the inside of the limiting groove (815) is provided with a limiting block (816) connected with the inner wall of the movable ring (808).

3. The purification device for nucleic acid extraction according to claim 1, characterized by: The top of the movable block (804) is connected with the bottom of the threaded sleeve (803), the movable groove (805) is annular, and the movable block (804) is slidably connected with the movable groove (805).

4. The purification device for extracting nucleic acid according to claim 1, characterized by: A test tube is placed in the placing barrel (801), and the top of the device body (1) is rotatably connected with a sealing cover (2).

5. The purification device for nucleic acid extraction according to claim 1, characterized by: An electric heating rod (3) is mounted on one side of the inside of the device body (1), and a temperature sensor (4) is mounted on the other side of the inside of the device body (1).

6. The purification device for nucleic acid extraction according to claim 1, characterized by: A rotating shaft (11) is connected to the lower part of the inside of the device body (1) through a bearing, and the top end of the rotating shaft (11) is provided with a rotating platform (9).

7. The purification device for nucleic acid extraction according to claim 6, characterized by: A motor (6) is also mounted on the lower part of the inside of the device body (1), and the output end of the motor (6) is connected with a gear (7).

8. The purification device for extracting nucleic acid according to claim 7, characterized by: A gear ring (10) is mounted on the outer wall of the rotating platform (9), and the gear (7) is engaged with the gear ring (10).

9. The purification device for nucleic acid extraction according to claim 1, characterized by: The movable sleeve (806) is slidably connected with the threaded column (802), and the outer wall of the threaded sleeve (803) is provided with anti-skid lines.

10. The purification device for nucleic acid extraction according to claim 1, characterized by: The clamping plate (813) and the silica gel pad (814) are both arc-shaped, and the clamping plate (813) and the connecting rod (812) are both slidably connected with the sliding seat (810) and the sliding rod (811) through the sliding seat (810).

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