Automatic balancing device

Abstract

The utility model relates to the technical field of cell sheet preparation, in particular to an automatic balancing device applied to a full-automatic liquid-based cell sheet preparation machine, which comprises a centrifugal turntable, a detection unit, a transfer module and a plurality of balancing blocks, sample bottle bearing seats are uniformly arranged on the periphery of the centrifugal turntable; the detection unit is used for detecting whether the sample bottles in the centrifugal turntable are trimmed or not and recording the sample bottle bearing seats needing to be trimmed; the transfer module comprises a mechanical arm and a transfer suction cup, and the transfer suction cup is fixedly installed on the mechanical arm; the mechanical arm drives the transfer suction cup to suck the balancing block and place the balancing block in the sample bottle bearing seat needing balancing. Through the cooperative arrangement of the transfer module and the detection unit, the automatic balancing device with the high automation degree is obtained, the centrifugal self-balancing function is achieved, manpower is reduced, and the working efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell slice technology field, concretely relates to a kind of automatic balancing device applied to full-automatic liquid-based cell slice machine. BACKGROUND

[0002] The existing full-automatic liquid-based cell slice technology using natural sedimentation method includes the step of centrifugal enrichment of cells. For example, patent CN211784689U provides a full-automatic pathological slide staining and slicing machine, which includes a body, a pump set, a centrifuge, a sample tank, a staining tank, a first suction nozzle placement tank, a second suction nozzle placement tank, a suction nozzle liquid taking arm, a staining agent adding and pumping arm, and an arm transverse movement module, making the operation of cell slicing and staining more convenient and efficient, and providing a good hardware equipment foundation for improving work efficiency and accuracy, so as to make the analysis of complex samples more effective.

[0003] The problem is that manual balancing is required during the centrifugation process, and too much human involvement cannot achieve true full automation.

[0004] Therefore, there is an urgent need for an automatic balancing device to solve the problem of too much human involvement during the centrifugation process. SUMMARY

[0005] The utility model provides an automatic balancing device to solve the problem of too much human involvement in related technologies.

[0006] To achieve the above-mentioned purpose, the utility model provides an automatic balancing device, which includes a centrifugal turntable, a detection unit, a transfer module, and a plurality of balancing blocks.

[0007] The centrifugal turntable is uniformly provided with sample bottle bearing seats on its outer periphery.

[0008] The detection unit detects whether the sample bottles in the centrifugal turntable are balanced and records the sample bottle bearing seats that need to be balanced.

[0009] The transfer module includes a mechanical arm and a transfer suction cup, which is fixedly installed on the mechanical arm. The transfer suction cup is driven by the mechanical arm to suck the balancing block and place it in the sample bottle bearing seat that needs to be balanced.

[0010] Further, the mechanical arm includes an X-arm, a Y-arm, and a Z-arm.

[0011] The X-arm is movably installed on one side of the slicing module, the Y-arm is movably installed on the X-arm, and the Z-arm is movably installed on the Y-arm.

[0012] Further, the detection unit includes a positioning sensor and a bottle detection sensor.

[0013] The positioning sensor comprises a component one and a component two, the component one is fixedly arranged on the centrifugal turntable and rotates with the centrifugal turntable, and the component one is used in cooperation with the component two to position the sample bottle carrier;

[0014] The bottle detection sensor is arranged on one side of the centrifugal turntable and is slightly higher than the sample bottle carrier, and the bottle detection sensor judges whether the sample bottle carrier has the sample bottle;

[0015] The positioning sensor and the bottle detection sensor cooperate to confirm the sample bottle carrier on which the balancing block needs to be placed.

[0016] Further, the balancing block is a columnar structure with the same weight as the sample bottle, and has a non-through insertion hole in the middle, and the insertion hole has a diameter slightly larger than the sleeve of the liquid suction and injection assembly.

[0017] Further, the balancing block is a columnar structure with the same weight as the three sample bottles, and has a non-through insertion hole in the middle, and the insertion hole has a diameter slightly larger than the sleeve of the liquid suction and injection assembly.

[0018] The beneficial effects of the present application are as follows:

[0019] Through the cooperation of the transfer module and the detection unit, an automatic balancing device with high automation degree is obtained, the centrifugal self-balancing function is realized, the manual work is reduced, and the work efficiency is improved. DRAWINGS

[0020] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed in the description of the embodiments of the present application will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0021] Figure 1 It is a schematic diagram of the full-automatic liquid-based cell preparation machine of the present application;

[0022] Figure 2 It is a schematic diagram of the full-automatic liquid-based cell preparation machine of the present application;

[0023] Figure 3 It is a schematic diagram of the full-automatic liquid-based cell preparation machine of the present application;

[0024] Figure 4 It is a schematic diagram of the full-automatic liquid-based cell preparation machine of the present application;

[0025] Figure 5 It is a schematic diagram of the transfer module of the full-automatic liquid-based cell preparation machine of the present application;

[0026] Figure 6 It is a schematic diagram of the liquid suction and injection mechanism of the full-automatic liquid-based cell preparation machine of the present application;

[0027] Figure 7 It is the schematic diagram of the liquid suction and injection assembly of the full-automatic liquid-based cell making machine of the utility model;

[0028] Figure 8 It is the schematic diagram of the waste liquid collecting device and waste liquid pouring device of the full-automatic liquid-based cell making machine of the utility model;

[0029] Figure 9 It is the schematic diagram of the waste liquid collecting device and waste liquid pouring device of the full-automatic liquid-based cell making machine of the utility model;

[0030] Figure 10 It is the schematic diagram of the waste liquid collecting device and waste liquid pouring device of the full-automatic liquid-based cell making machine of the utility model;

[0031] Figure 11 It is the schematic diagram of the full-automatic liquid-based cell making machine of the utility model;

[0032] Figure 12 It is the schematic diagram of the full-automatic liquid-based cell making machine of the utility model;

[0033] Among them:

[0034] 1, full-automatic liquid-based cell preparation machine; 2, transfer module; 3, cell enrichment module; 4, pipette tip module; 5, preparation module; 6, mechanical arm; 7, suction and injection mechanism; 8, suction and injection assembly; 9, sleeve; 10, suction needle; 11, injection needle; 12, centrifugal turntable; 13, first driving device; 14, waste liquid pouring device; 15, automatic balancing device; 16, sample bottle bearing seat; 17, shaft; 18, sample bottle; 19, sample bottle push rod; 20, detection unit; 21, balancing block; 22, transfer suction disc; 23, X arm; 24, Y arm; 25, Z arm; 26, upper end of suction needle; 27, first waste liquid pipeline; 28, waste liquid bottle; 29, first pump; 30, first valve; 31, controller; 32, upper end of injection needle; 33, reagent pipeline; 34, reagent bottle; 35, second pump; 36, second valve; 37, lower end of suction needle; 38, horizontal push rod; 39, vertical push rod; 40, first vertical arm; 41, first horizontal arm; 42, second driving device; 43, second vertical arm; 44, second horizontal arm; 45, third driving device; 46, fixed block; 47, waste liquid collection device; 48, waste liquid receiving disc; 49, second waste liquid pipeline; 50, disc bottom; 51, inner disc wall; 52, outer disc wall; 53, recessed space; 54, waste liquid port; 55, third valve; 56, non-slip pad; 57, positioning sensor; 58, bottle detection sensor; 59, assembly one; 60, assembly two; 61, pipette tip seat; 62, photoelectric sensor; 63, pipette tip position; 64, workbench; 65, preparation clamp assembly; 66, work station; 67, slide holder; 68, sedimentation bin; 69, slide; 70, sealing rubber ring; 71, clamp; 72, solid waste collection module; 73, pipe removal assembly; 74, waste collection box; 75, support plate; 76, pipette removal plate; 77, avoidance part; 78, disposable pipette tip; 79, jack; 80, flushing pipe. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0036] As Figures 1-4The utility model provides a kind of full-automatic liquid-based cell preparation machine 1, comprising: transfer module 2, cell enrichment module 3, pipette head module 4 and preparation module 5, cell enrichment module 3, pipette head module 4 and preparation module 5 are sequentially arranged, and transfer module 2 is arranged above cell enrichment module 3, pipette head module 4 and preparation module 5.Transfer module 2 includes mechanical arm 6 and suction and injection mechanism 7, and suction and injection mechanism 7 is fixedly installed on mechanical arm 6.Cell enrichment module 3 includes: centrifugal turntable 12, waste liquid pouring device 14, self-aligning assembly 15 and first driving device 13.The outer periphery of centrifugal turntable 12 is uniformly provided with sample bottle carrier 16, for carrying sample bottle 18.Sample bottle carrier 16 upper two sides are connected with centrifugal turntable 12 shaft, and sample bottle carrier 16 can swing around shaft 17.Waste liquid pouring device 14 is composed of sample bottle push rod 19, and sample bottle push rod 19 can push sample bottle carrier 16, so that sample bottle carrier 16 swings along shaft 17.

[0037] Mechanical arm 6 on transfer module 2 is used to drive suction and injection mechanism 7 to move inside full-automatic liquid-based cell preparation machine 1, to complete each step process of preparation between different modules, including mixing sample, transferring, sample, adding reagent and suctioning and discharging waste liquid.Centrifugal turntable 12 on cell enrichment module 3 is used to enrich cells in sample, and waste liquid pouring device 14 is used to discharge supernatant after centrifugation, so that cells of diagnostic significance are centrifuged and precipitated to remain at the bottom of sample bottle.Preparation module 5 is used for sedimentation preparation and staining of cell sample, and pipette head module 4 is used to load disposable pipette head 78.

[0038] Specifically, first, cell enrichment module 3 deposits cell sample stored in sample bottle 18 at the bottom tip position of sample bottle 18 through centrifugal effect, i.e., centrifugal turntable 12 rotates, sample bottle carrier 16 swings to horizontal state around rotation shaft 17 under the action of centrifugal force, centrifugal radius increases, centrifugal effect is good, and precipitate is gathered at the sharp bottom of sample bottle 18 after centrifugation, to facilitate subsequent mixing.

[0039] Then, waste liquid pouring device 14 pushes sample bottle 18, sample bottle 18 swings around shaft 17 in sample bottle carrier 16, so that sample bottle 18 swings to horizontal state, or even makes sample bottle 18 bottle mouth lower than bottle bottom position, supernatant in sample bottle 18 flows out automatically under the action of gravity, and then waste liquid pouring device 14 resets, and sample bottle 18 automatically restores vertical state.This step only leaves cells of diagnostic significance in sample bottle 18, to achieve the purpose of enriching cells.

[0040] Then, the suction and injection mechanism 7 on the transfer module 2 picks up the disposable pipette tip 78 in the pipette tip module 4 by plugging, and injects liquid into the enriched cells, and resuspends the cells in the liquid, and mixes the cells. The resuspended cells are transferred from the enrichment module 3 to the slide preparation module 5 by using the disposable pipette tip 78.

[0041] Finally, the suction and injection mechanism 7 on the transfer module 2 injects reagents into the slide preparation module 5 and pumps out waste liquid, and assists the slide preparation module 5 to complete slide preparation and staining. It can be understood that the slide preparation generally refers to the sedimentation preparation, and the staining generally refers to the cell staining.

[0042] Through the above-mentioned embodiment, the cooperation of the centrifugal rotor 12 of the cell enrichment module 3 and the waste liquid pouring device 14 can automatically complete the centrifugation and the operation of pouring the supernatant without manual intervention, and the enriched cells are obtained.

[0043] Through the above-mentioned embodiment, the transfer module 2 cooperates with the disposable pipette tip 78 to complete the resuspension, mixing and transfer of the enriched cells, the suction and injection assembly 8 of the transfer module 2 injects different reagents and pumps out the waste liquid in each step of the slide preparation process, and the slide preparation and staining are completed.

[0044] The above-mentioned embodiment has the advantages that the mechanical arm for sucking the centrifugal supernatant is abandoned, the waste liquid pouring device 14 with simple structure is adopted, the supernatant is naturally poured, and only one mechanical arm 6 is reserved for the resuspension, mixing and transfer of the cells, so that the full-automatic liquid-based cell slide preparation machine has compact and simple structure and is easy to maintain. The independent waste liquid pouring device 14 can release the transfer module 2 from the work of pumping out the waste liquid, so that the waste liquid pouring and the sample transfer can be simultaneously performed, and the work efficiency is improved.

[0045] Further, as shown in Figures 8-11 The self-leveling assembly 15 includes a detection unit 20, a plurality of leveling blocks 21 and a transfer suction disc 22; the detection unit 20 detects whether the sample bottle in the centrifugal rotor 12 is leveled, and records the sample bottle carrier 16 that needs to be leveled, and the transfer suction disc 22 is fixed on the mechanical arm 6, and the transfer suction disc 22 is driven by the mechanical arm 6 to suck the leveling block 21 and place it in the sample bottle carrier 16 that needs to be leveled.

[0046] The self-leveling assembly 15 is used for automatic leveling before centrifugation, reduces manual work, and improves the degree of automation.

[0047] The specific process of automatic balancing is as follows: the sample bottle 18 storing the cell sample is placed in the sample bottle bearing seat 16 of the cell enrichment module 3, the detection unit 20 of the self-balancing assembly 15 detects whether the sample bottle 18 is balanced, and records the sample bottle bearing seat 16 that needs to be balanced and transmits the information to the controller 31 (not shown in the figure); the controller 31 controls the mechanical arm 6 to drive the transfer suction disc 22 to suck the balancing block 21 and place it in the sample bottle bearing seat 16 that needs to be balanced, thereby realizing the automatic balancing function.

[0048] Through the above-mentioned embodiments, the cooperation of the self-balancing assembly and the transfer module 2 automatically completes the balancing work of the cell enrichment module before centrifugal enrichment of cells, without manual participation, and has high automation degree.

[0049] In summary, through the above-mentioned embodiments, the cell enrichment module 3, the transfer module 2 and the slide preparation module 5 work together to complete the whole process of preparing a slide specimen from a sample collected in a laboratory with little manual participation, and have high automation degree. The full-automatic liquid-based cell preparation machine has compact and simple structure and is easy to maintain.

[0050] Further, as shown in Figures 5-7 The mechanical arm 6 comprises an X arm 23, a Y arm 24 and a Z arm 25. The X arm 23 is movably installed on one side of the slide preparation module, the Y arm 24 is movably installed on the X arm 23, and the Z arm 25 is movably installed on the Y arm 24. The liquid suction mechanism 7 is fixedly arranged on the Z arm 25.

[0051] Specifically, the full-automatic liquid-based cell preparation machine is provided with a vertical plate on one side of the slide preparation module. The one side is that the vertical plate can be located on the side of the slide preparation module, and at this time, the vertical plate is parallel to the line connecting the cell enrichment module 3, the liquid transfer gun head module 4 and the slide preparation module 5. Alternatively, the vertical plate can be arranged in sequence with the cell enrichment module 3, the liquid transfer gun head module 4 and the slide preparation module 5, and at this time, the vertical plate is perpendicular to the line connecting the cell enrichment module 3, the liquid transfer gun head module 4 and the slide preparation module 5.

[0052] The first motor, the first guide rail and the first belt are fixedly installed on the vertical plate, and the first motor is fixedly arranged at one end of the vertical plate. The first belt is horizontally arranged, one end of which passes through a first driving wheel on the output shaft of the first motor, and the other end of which passes through a first driven wheel fixedly arranged on the vertical plate. The first motor can drive the first belt to move horizontally and reciprocally. The first guide rail is fixedly arranged on the vertical plate, and the first guide rail is arranged in parallel with the first belt.

[0053] The X arm 23 is installed on the first belt and the first guide rail.

[0054] The X arm 23 comprises an X sliding block (not shown in the figure) and an X support (not shown in the figure).

[0055] The X slider is fixedly arranged at one end of the first belt and movably arranged at the other end of the first guide rail, and the X slider can move along the first guide rail horizontally (in the X direction) under the driving of the first belt.

[0056] The first motor drives the first belt to move horizontally, the moving first belt drives the X slider to move horizontally along the first guide rail, and the moving X slider drives the X support to move horizontally, thereby realizing the movement of the X arm in the X direction.

[0057] The X support is further fixedly provided with a second motor, a second guide rail and a second belt, the second motor is fixedly arranged at one end of the X support, and the second belt is arranged horizontally and perpendicularly to the first belt; one end of the second belt is wound around a second driving wheel on an output shaft of the second motor, and the other end is wound around a second driven wheel fixedly arranged on the X support; the second motor can drive the second belt to move horizontally. The second guide rail is fixedly arranged on the X support, and the second guide rail is arranged in parallel with the second belt.

[0058] The Y arm 24 is arranged on the second belt and the second guide rail.

[0059] The Y arm 24 comprises a Y slider (not shown in the figure) and a Y support (not shown in the figure).

[0060] The Y slider is fixedly arranged at one end of the second belt and movably arranged at the other end of the second guide rail, and the Y slider can move along the second guide rail horizontally (in the Y direction) under the driving of the second belt. The Y support is fixedly connected to the Y slider.

[0061] The second motor drives the second belt to move horizontally, the moving second belt drives the Y slider to move horizontally along the second guide rail, and the moving Y slider drives the Y support to move horizontally, thereby realizing the movement of the Y arm in the Y direction.

[0062] Since the Y arm 24 is movably arranged on the X arm 23, the Y arm can move in the X direction under the driving of the X arm, that is, the Y arm can move in both the X and Y directions.

[0063] The Y support is further fixedly provided with a third motor, a third guide rail and a third belt, the third motor is fixedly arranged at one end of the Y support; the third belt is arranged horizontally and longitudinally and perpendicularly to the second belt; one end of the third belt is wound around a third driving wheel on an output shaft of the third motor, and the other end is wound around a third driven wheel fixedly arranged on the Y support; the third motor can drive the third belt to move horizontally. The third guide rail is fixedly arranged on the Y support, and the third guide rail is arranged in parallel with the third belt.

[0064] The Z arm 25 is arranged on the third belt and the third guide rail.

[0065] The Z arm 25 comprises a Z slider (not shown in the figure) and a Z support (not shown in the figure).

[0066] One end of the Z slider is fixedly arranged on the third belt, and the other end is movably arranged on the third guide rail, so that the Z slider can move reciprocatingly along the third guide rail in the Z direction under the driving of the third belt.

[0067] The third motor drives the third belt to move reciprocatingly horizontally, the moving third belt drives the Z slider to move reciprocatingly horizontally along the third guide rail, and the moving Z slider drives the Z support to move reciprocatingly horizontally, so as to realize the movement of the Z arm in the Z direction.

[0068] Since the Z arm 24 is movably arranged on the Y arm 23, the Z arm can realize the movement of the Y arm in the X and Y directions under the driving of the Y arm, that is, the Z arm can move in the X, Y and Z directions.

[0069] The liquid suction and injection mechanism 7 is fixedly arranged on the Z support and can move in the X, Y and Z directions under the driving of the Z arm.

[0070] That is to say, through the arrangement of the X arm 23, the Y arm 24 and the Z arm 25, the liquid suction and injection mechanism 7 can freely move in the working space, realize the grasping of the disposable pipette tip 78 at any position and the transfer of the sample in the sample bottle at different positions to the corresponding sample bottle, and realize the suction and injection operation on the sample bottle at any position.

[0071] That is to say, through the arrangement of the X arm 23, the Y arm 24 and the Z arm 25, the liquid suction and injection mechanism 7 can freely move in the working space, realize the grasping of the disposable pipette tip 78 at any position and the transfer of the sample in the sample bottle at different positions to the corresponding sample bottle, and realize the suction and injection operation on the sample bottle at any position.

[0072] Further, as shown in the figure, the liquid suction and injection mechanism 7 comprises a plurality of liquid suction and injection assemblies 8, the liquid suction and injection assembly 8 comprises a sleeve 9, a liquid suction needle 10 and a liquid injection needle 11 inside the sleeve 9, and the liquid suction needle 10 penetrates through the sleeve 9 and extends out. Figures 5-7

[0073] Firstly, the liquid suction needle 10 is used for pumping and discharging the waste liquid generated in each step of the sample preparation process out of the sample preparation module 5, and the liquid injection needle 11 is used for adding different reagents required in each step of the sample preparation process into the sample preparation module 5 to assist the sample preparation.

[0074] Specifically, the liquid suction needle 10 and the liquid injection needle 11 are both capillary steel pipes with a diameter of 1-5 mm, which are easy to bend and deform, thereby affecting the liquid suction and injection.

[0075] ​The liquid injection needle 11 is used for liquid injection. In order to avoid cross contamination between different samples, the liquid injection needle 11 needs to be kept clean, and therefore the whole liquid injection needle 11 is placed in the sleeve 9. This arrangement does not affect the liquid injection function, and enables the sleeve 9 to protect the liquid injection needle 11 from deformation to the maximum extent, and prevents the liquid injection needle 11 from being contaminated when the suction and injection mechanism 7 extends into various sample containers.

[0076] In order to ensure that the liquid suction needle 10 successfully sucks the waste liquid while avoiding cross contamination of the liquid injection needle 11, in addition to the above-mentioned measure of placing the whole liquid injection needle 11 in the sleeve 9, the liquid suction needle 10 is arranged to extend through the sleeve 9 and protrude. In this way, the liquid suction needle 10 can successfully suck the waste liquid, and when sucking the waste liquid, only the liquid suction needle 10 that protrudes from the sleeve 9 contacts the waste liquid, and the sleeve 9 and the liquid injection needle 11 inside the sleeve 9 do not contact the waste liquid, thereby avoiding cross contamination.

[0077] Furthermore, the liquid injection needle 11 can be used to clean the liquid suction needle 10 when injecting liquid. Specifically, the liquid suction needle 10 and the liquid injection needle 11 are arranged in the sleeve, and the liquid suction needle 10 and the liquid injection needle 11 are tightly attached together, and the liquid outlet of the liquid injection needle 11 contacts the outer wall of the liquid suction needle 10. When the liquid injection needle 11 injects liquid, the reagent flows out of the liquid outlet of the liquid injection needle 11, flows down along the outer wall of the liquid suction needle 10, and the clean reagent flushes the liquid suction needle 10, achieving the effect of cleaning the liquid suction needle 10.

[0078] Secondly, the suction and injection mechanism 7 picks up the disposable pipette tip 78 by inserting the sleeve 9, and after resuspending and mixing the cell sample by using the disposable pipette tip 78, the cell sample is transferred to the slide preparation module 5.

[0079] Specifically, the sleeve 9 is moved to the pipette tip module 4 under the action of the mechanical arm 6, and is lowered, and the sleeve 9 is inserted into the disposable pipette tip 78 to pick up the disposable pipette tip 78, and then returns to the cell enrichment module 3 to inject liquid into the centrifuged cells. The suction and injection mechanism 7 generates continuous negative pressure and positive pressure to suck and discharge the cell sample into and out of the disposable pipette tip 78, thereby resuspending and mixing the cell sample, and finally the cell sample is sucked into the disposable pipette tip 78. Under the action of the mechanical arm 6, the cell sample is moved to the slide preparation module 5, and the cell sample is injected into the slide preparation module 5, completing the transfer of the cell sample from the cell enrichment module 3 to the slide preparation module 5.

[0080] Further, as shown in FIG. 6, the sleeve 9 is provided with a plurality of liquid injection holes 91, and the liquid injection needle 11 is arranged to extend through the liquid injection holes 91. Figure 6As shown, the upper end 26 of the suction needle 10 is connected with the waste liquid bottle 28 through the first waste liquid pipeline 27, the first pump 29 and the first valve 30 are arranged on the first waste liquid pipeline 27, and the lower end of the suction needle is bent. The upper end 32 of the injection needle 11 is connected with the reagent bottle 34 through the reagent pipeline 33, the second pump 35 and the second valve 36 are arranged on the reagent pipeline 33. Further comprising a controller 31, the controller 31 is electrically connected with the first pump 29, the first valve 30, the second pump 35 and the second valve 36, and the controller 31 controls the opening and closing of the first pump 29, the first valve 30, the second pump 35 and the second valve 36.

[0081] Specifically, the controller 31 controls the opening and closing of the second pump 35 and the second valve 36 on the reagent pipeline 33, so that the corresponding reagent is added to the sample bottle 18 of the cell enrichment module 3 or the area of the slide preparation module 5 through the injection needle 11, and the corresponding slide preparation step is completed.

[0082] The controller 31 controls the opening and closing of the first pump 29 and the first valve 30 on the first waste liquid pipeline 27, so that the suction needle 10 sucks the waste liquid in the slide preparation module 5, in order to carry out the subsequent slide preparation step.

[0083] As a possible implementation, as shown in Figure 7 The injection needle 11 is 1-5, which is used to add different reagents respectively.

[0084] Specifically, cell slide and staining often need 1-5 kinds of reagents with different physical and chemical properties, and different pipelines are needed for delivery to prevent reagents from mixing and affecting the physical and chemical properties of the reagents, causing slide failure and affecting the staining effect.

[0085] This embodiment integrates 1-5 injection needles 11 with the suction needle 10 in one sleeve, which meets the needs of adding multiple different reagents under the premise of compact structure.

[0086] As a possible implementation, as shown in Figure 7 The lower end 37 of the suction needle is bent.

[0087] Specifically, when the suction needle 10 sucks liquid, the bent lower end can be as close as possible to the cell layer on the slide 69, so as to suck as much waste liquid above the cell layer as possible, and at the same time, avoid the sharp lower end 37 directly contacting the cell layer and damaging the cell layer.

[0088] At the same time, when the injection needle 11 injects liquid, the liquid flows down along the suction needle 10 after flowing out of the injection needle 11, and under the guidance of the suction needle 10, the liquid flows obliquely along the bent top end of the suction needle 10, and will not vertically and directly impact the cell layer, preventing the cell layer from being damaged.

[0089] Further, as shown in Figure 5 The suction and injection assembly 8 is 1-5 groups, which are arranged in a line on the mechanical arm 6.

[0090] Specifically, the pipetting assembly 8 is used for adding reagent to the sample and pumping waste liquid. A set of pipetting assemblies 8 can process one sample, and 1-5 sets of pipetting assemblies 8 can simultaneously process 1-5 samples, thereby improving the sample processing efficiency.

[0091] As a possible implementation, as shown in Figures 1-4 The pipetting mechanism 7 generates continuous positive pressure and negative pressure to repeatedly suck and discharge the sample in the sample bottle 18 by the disposable pipette tip 78, so as to achieve the purpose of resuspending and mixing the sample.

[0092] It can be understood that there are various specifications of commercially available disposable pipette tips. The pipetting mechanism 7 of the present embodiment can set a suitable negative pressure value according to the specification of the disposable pipette tip, so that the volume of the sample sucked by the disposable pipette tip is adapted to its specification, that is, the sample is only in the disposable pipette tip and will not be sucked into the inner cavity of the sleeve 9. Each disposable pipette tip only processes the sample in one sample bottle and is discarded after use. The sample only contacts the disposable pipette tip and will not contact the pipetting mechanism, thereby avoiding contamination.

[0093] Further, as shown in Figures 8-10 The sample bottle push rod 19 includes a horizontal push rod 38 and a vertical push rod 39, and the horizontal push rod 38 is located above the vertical push rod 39.

[0094] The horizontal push rod 38 is located at the upper part of the sample bottle carrier 16 and includes a first vertical arm 40, a first horizontal arm 41, and a second driving device 42. The first vertical arm 40 and the first horizontal arm 41 are connected in a T shape, the first horizontal arm 41 is perpendicular to the radius of the centrifugal turntable 12, and the second driving device 42 is connected with the first vertical arm 40 and can drive the horizontal push rod 38 to move toward the center of the centrifugal turntable 12, so that the first horizontal arm 41 contacts the upper part of the sample bottle in the sample bottle carrier 16.

[0095] The vertical push rod 39 is located below the sample bottle carrier 16 and includes a second vertical arm 43, a second horizontal arm 44, and a third driving device 45. The second vertical arm 43 and the second horizontal arm 44 are connected in a T shape, the second horizontal arm 44 is perpendicular to the radius of the centrifugal turntable 12, and the third driving device 45 is connected with the second vertical arm 43 and can drive the vertical push rod 39 to move in a vertical upward direction, so that the second horizontal arm 44 contacts the sample bottle carrier 16.

[0096] After the horizontal push rod is pushed to contact the upper part of the side wall of the sample bottle, the sample bottle can be pushed in the horizontal direction, so that the sample bottle drives the sample bottle carrier to swing and tilt around the shaft. After the vertical push rod is pushed to contact the lower part of the side wall of the tilted sample bottle carrier, the sample bottle carrier can be pushed in the vertical direction, so that the sample bottle carrier drives the sample bottle to continue to swing and tilt around the shaft.

[0097] Traditional liquid-based cell preparation machines use a suction method to discharge waste liquid (supernatant after centrifugation), that is, through a mechanical arm to drive a gas-liquid pipeline into each sample bottle, to generate negative pressure to suck the waste liquid in the sample bottle and transfer it into a waste liquid bottle. This method must have a pump, valve, pipeline and mechanical arm to cooperate, and the mechanical mechanism is complex, with many accessories and a high failure rate.

[0098] Therefore, the application discards this suction type waste liquid discharge method and uses a simple structure of a pouring device to push the sample bottle to make the waste liquid automatically flow out under the action of gravity.

[0099] Specifically, when the centrifugation is completed and the waste liquid (supernatant) needs to be poured, the second driving device 42 drives the first vertical arm 40 of the horizontal push rod 38 to move and drives the first horizontal arm 41 to move towards the center of the centrifugal turntable 12, contacts the upper part of the sample bottle carrier 16, and continues to move to push the sample bottle carrier 16 to swing around the rotation shaft 17, so that the sample bottle carrier 16 and the sample bottle 18 placed therein swing and tilt around the rotation shaft 17, and at this time the lower side of the sample bottle carrier 16 is lifted to above the vertical push rod. Then the third driving device 45 drives the second vertical arm 43 of the vertical push rod 39 to move upwards, and drives the second horizontal arm 44 to move upwards, contacts the lower side of the tilted sample bottle carrier 16, and continues to move to push the sample bottle carrier 16 to swing along the rotation shaft 17, drives the sample bottle 18 to continue to swing around the rotation shaft 17, until the sample bottle opening position is lower than the position of the bottle bottom, and the waste liquid flows out of the sample bottle 18 under the action of gravity. The horizontal push rod 38 and the vertical push rod 39 cooperate with each other to realize the discharge of the waste liquid in the sample bottle.

[0100] After the waste liquid is discharged, the vertical push rod 39 retreats to reset, the horizontal push rod 38 retreats to reset, and the sample bottle 18 and the sample bottle carrier 16 automatically return to the vertical state under the action of gravity.

[0101] It can be understood that the horizontal push rod 38 and the vertical push rod 39 constituting the sample bottle push rod 19 are both one, which can be arranged at any position on the outer periphery of the centrifugal turntable 12.

[0102] Specifically, the sample bottle moves to the position of the sample bottle push rod 19 in turn with the rotation of the centrifugal turntable 12, and is pushed down by the sample bottle push rod 19 to pour out the waste liquid. The sample bottle push rod 19 arranged at any position on the outer periphery of the centrifugal turntable 12 can push down any sample bottle through the rotation of the centrifugal turntable 12 to complete the waste liquid pouring work.

[0103] As a possible implementation, the height of the sample bottle 18 is greater than the height of the inner cavity of the sample bottle carrier 16, and when the sample bottle 18 is placed in the sample bottle carrier 16, the upper part of the sample bottle 18 is higher than the sample bottle carrier 16. The horizontal push rod 38 of the sample bottle push rod 19 can push the upper part of the sample bottle 18 in the sample bottle carrier 16, so that the sample bottle 18 drives the sample bottle carrier 16 to swing along the shaft 17.

[0104] The benefit of such an arrangement is that when pouring waste liquid, the horizontal push rod 38 can directly push the sample bottle without pushing the sample bottle carrier 16, so that the sample bottle carrier 16 can be connected to the shaft of the centrifugal disc 12 at the uppermost part, so that the center of gravity of the sample bottle carrier 16 is far below the shaft, and after the external force is removed, it is beneficial to the sample bottle carrier 16 to quickly return to the vertical state under the action of gravity.

[0105] Further, as shown in Figures 8-10 The middle part of the second cross arm 44 is provided with a fixing block 46, and the size of the fixing block 46 is adapted to the distance between the two sample bottle carriers 16, which is used to fix the sample bottle carrier 16.

[0106] It can be understood that the sample bottle push rod 19 can push one sample bottle at a time to complete the waste liquid pouring, or can push two sample bottles at a time to complete the waste liquid pouring.

[0107] Pushing one sample bottle at a time is low in efficiency. Pushing two sample bottles at a time can improve the efficiency by one time. Both of these two methods have a problem, that is, the centrifugal disc 12 itself is rotatable, and when the sample bottle push rod 19 pushes the sample bottle, uneven stress will cause the centrifugal disc to rotate, causing the sample bottle to slip off the sample bottle push rod 19, affecting the waste liquid pouring.

[0108] The embodiment is provided with a fixing block 46 in the middle part of the second cross arm 44, and the size of the fixing block 46 is adapted to the distance between the two sample bottle carriers 16, when the second cross arm 44 of the vertical push rod 39 contacts the sample bottle 18, the fixing block 46 is clamped between the two sample bottles 18, and the sample bottle 18 is fixed to avoid the centrifugal disc 12 from continuing to rotate under the action of inertia, and the sample bottle 18 is stabilized, so that the pouring of the centrifugal supernatant (i.e. waste liquid) is more convenient and easy to operate, and the waste liquid treatment is facilitated.

[0109] Further, as shown in Figures 8-10 It further includes a waste liquid collecting device 47. The waste liquid collecting device 47 includes a waste liquid receiving disc 48, a second waste liquid pipeline 49 and a waste liquid bottle 28.

[0110] The waste liquid receiving disc 48 is arranged below the centrifugal disc 12 and is annular. The waste liquid receiving disc 48 comprises a disc bottom 50 and an inner disc wall 51 and an outer disc wall 52. The inner disc wall 51 is located at an inner periphery of the disc bottom 50, and the outer disc wall 52 is located at an outer periphery of the disc bottom 50. The inner disc wall 51 and the outer disc wall 52 protrude from the disc bottom 50. The disc bottom 50, the inner disc wall 51 and the outer disc wall 52 jointly form an annular recessed space 53. The recessed space 53 can be used to receive waste liquid. The disc bottom 50 is provided with a waste liquid port 54 which penetrates the disc bottom 50 vertically. The lower end of the waste liquid port 54 is connected to the waste liquid bottle 28 through the second waste liquid pipeline 49. A third valve 55 is arranged on the pipeline and is controlled by the controller 31 to open and close.

[0111] The waste liquid collecting device 47 is used to collect the supernatant (waste liquid) generated after centrifugation of the cell enrichment module 3.

[0112] Specifically, after the waste liquid is poured out by the waste liquid pouring device 14, the waste liquid flows into the waste liquid receiving disc 48. The third valve 55 is opened, and the waste liquid flows into the waste liquid bottle 28 through the waste liquid port 54 and the second waste liquid pipeline 49.

[0113] The third valve 55 is normally closed and is opened only when the waste liquid is poured. On the one hand, this can reduce the pollution of the environment caused by the volatilization of waste liquid in the waste liquid bottle into the environment. On the other hand, this can provide a sealed environment for the pipeline system of the tablet machine, which is convenient for the generation of negative pressure and positive pressure in the pipeline system.

[0114] As a possible implementation, the waste liquid port 54 is located below the sample bottle push rod 19. Such an arrangement is conducive to the rapid flow of waste liquid into the waste liquid bottle, shortens the time for which the waste liquid stays in the waste liquid receiving disc 48, and reduces environmental pollution.

[0115] As a possible implementation, as shown in Figures 8-10 The outer disc wall 52 is provided with a non-slip pad 56 at a position corresponding to the sample bottle push rod group. The non-slip pad 56 is used to limit the sample bottle 18 after the sample bottle push rod group pushes the sample bottle 18 to the horizontal position.

[0116] It can be understood that the sample bottle 18 is naturally placed in the sample bottle carrier 16 without a limiting device. When the sample bottle 18 is tilted to the lowest position, the sample bottle 18 contacts the upper surface of the outer disc wall 52 of the waste liquid receiving disc 48. At this time, the bottle opening is lower than the bottle bottom, and the sample bottle 18 is easy to slide out of the sample bottle carrier 16, which leads to the failure of sample transfer due to the sliding out of the sample bottle. By arranging the non-slip pad with a large friction coefficient at the position where the sample bottle 18 contacts the upper surface of the outer disc wall 52 of the waste liquid receiving disc 48, the sample bottle 18 can be prevented from sliding out of the sample bottle carrier 16.

[0117] As a possible implementation, as shown in Figures 8-10As shown, the flushing pipe is arranged above the waste liquid outlet 54, and is used to clean the waste liquid outlet 54, prevent the waste liquid outlet 54 from being blocked by chemical reagent crystallization, and prevent the waste liquid outlet 54 from being corroded by residual chemical reagent.

[0118] As a possible implementation, as shown in Figures 1-4 The cell enrichment module 3 has a folding sealing cover.

[0119] When the sample bottle is placed, the sealing cover is opened, and the operation of the experimenter is not affected.

[0120] When centrifugation is performed, the sealing cover is closed, and the cell enrichment module 3 is closed, so that the sample or the sample bottle is prevented from being thrown out of the cell enrichment module 3 during centrifugation, and the aerosol generated during centrifugation is prevented from volatilizing into the environment, thereby protecting the health of the experimenter.

[0121] The folding sealing cover occupies a small space, and the size of the full-automatic liquid-based cell preparation machine 1 can be reduced as a whole.

[0122] Further, as shown in Figures 1-4 The detection unit 20 includes a positioning sensor 57 and a bottle detection sensor 58. The positioning sensor 57 includes a component one 59 and a component two 60. The component one 59 is fixedly arranged on the centrifugal turntable 12 and rotates with the centrifugal turntable 12. The component one 59 is used in cooperation with the component two 60 to position the sample bottle carrier seat 16.

[0123] The bottle detection sensor 58 is arranged on one side of the centrifugal turntable 12 and is slightly higher than the sample bottle carrier seat 16. The bottle detection sensor 58 determines whether the sample bottle carrier seat 16 has a sample bottle.

[0124] The positioning sensor 57 and the bottle detection sensor 58 cooperate to confirm the sample bottle carrier seat 16 on which the calibration block 21 needs to be placed.

[0125] Specifically, the installation positions of the bottle detection sensor 58 and the positioning sensor 57 can be pre-set, so that the bottle detection sensor 58 and the positioning sensor 57 are circumferentially different by at least one working position. The position of each sample bottle carrier seat 16 is the working position.

[0126] Taking the centrifugal turntable 12 with 24 sample bottle carrier seats 16 as an example, the positioning sensor 57 and the bottle detection sensor 58 are separated by 11 working positions.

[0127] When the assembly one 59 rotates with the centrifugal turntable 12 and passes through the assembly two 60, the assembly two 60 receives the signal of the assembly one 59 and transmits the signal to the controller 31, and records the sample bottle carrier seat 16 corresponding to the radius of the assembly one as the No. 1 sample bottle carrier seat. At this time, the bottle detection sensor 58 detects the sample bottle carrier seat 16 as the No. 13 sample bottle carrier seat. The bottle detection sensor 58 starts to detect whether there is a sample bottle 18 in the No. 13 sample bottle carrier seat and transmits the detection information to the controller 31. With the rotation of the centrifugal turntable 12, the bottle detection sensor 58 detects whether there is a sample bottle 18 in each sample bottle carrier seat in turn and transmits the detection information to the controller 31, until the centrifugal turntable 12 rotates one round, and the state detection of the sample bottles 18 in all sample bottle carrier seats 16 is completed. The controller 31 compares the information of the sample bottles 18 in the symmetrical sample bottle carrier seats 16 according to the received information, and makes a judgment on whether to balance or not, and records the information of the sample bottle carrier seats 16 that need to be balanced.

[0128] Further, as shown in Figure 11 The balancing block 21 is a columnar structure with the same weight as the sample bottle 18, and has a non-through insertion hole 79 in the middle, and the diameter of the insertion hole 79 is slightly larger than the diameter of the sleeve 9 of the liquid suction and injection assembly 8.

[0129] Specifically, the controller 31 controls the transfer module 2 to move above the balancing block 21, and then moves the liquid suction and injection mechanism 7 downward, so that the sleeve 9 penetrates into the insertion hole 79 of the balancing block 21. After the suction disc on the sleeve 9 contacts the top end of the balancing block 21, the liquid suction and injection mechanism 7 sucks vacuum to firmly suck and inject the balancing block 21, and then moves the balancing block 21 to the sample bottle carrier seat 16 that needs to be balanced under the driving of the transfer module 2, and completes the balancing. After the centrifugation is completed, the transfer module 2 transfers the balancing block 21 to the storage seat.

[0130] As a possible implementation, the balancing block is a columnar structure with the same weight as three sample bottles, and has a non-through insertion hole in the middle, and the diameter of the insertion hole is slightly larger than the diameter of the sleeve of the liquid suction and injection assembly.

[0131] The purpose of such arrangement is that the sample bottles 18 can be placed along the sample positions in sequence when placed manually, and there is no need to deliberately pay attention to balancing. After the self-balancing assembly 15 detects the information of the sample bottles 18, the balancing block 21 is automatically placed in the sample bottle carrier seat 16 that needs to be balanced to balance.

[0132] Taking 12 sample bottles 18 as an example, the 12 sample bottles 18 are placed in the sample bottle seats 1-12 in turn. After the self-balancing assembly 15 detects the information of the sample bottles 18, the state information of the sample bottles 18 is sent to the controller 31, the controller 31 controls the transfer module 2 to move above the balancing blocks 21, the suction and injection mechanism 7 is lowered to make the sleeve 9 deeply insert into the insertion hole 79 of the balancing block 21, after the suction disc on the sleeve 9 contacts the top end of the balancing block 21, the suction and injection mechanism 7 is vacuumized to firmly suck and inject the balancing block 21, and under the driving of the transfer module 2, the four balancing blocks 21 are transferred to the sample bottle seats 16 of Nos. 14, 17, 20 and 23 to complete the balancing, that is, the three sample bottles 18 in the sample bottle seats 1, 2 and 3 are balanced with the balancing block 21 in the sample bottle seat 14, the three sample bottles 18 in the sample bottle seats 4, 5 and 6 are balanced with the balancing block 21 in the sample bottle seat 17, the three sample bottles 18 in the sample bottle seats 7, 8 and 9 are balanced with the balancing block 21 in the sample bottle seat 20, and the three sample bottles 18 in the sample bottle seats 10, 11 and 12 are balanced with the balancing block 21 in the sample bottle seat 23. After centrifugation is completed, the transfer module 2 transfers the balancing blocks 21 to the storage seats.

[0133] Further, as shown in FIG. 6, the pipette tip module 4 comprises a pipette tip seat 61 and a photoelectric sensor 62. The pipette tip seat 61 is used to carry a disposable pipette tip 78; the pipette tip seat 61 is provided with a pipette tip position 63 which is a circular hole, and the disposable pipette tip 78 can be stably placed in the circular hole of the pipette tip position 63. Figures 1-4

[0134] It can be understood that the disposable pipette tip 78 can be installed on the sleeve 9 of the transfer module 2 in a plug-in manner.

[0135] Specifically, the suction and injection mechanism 7 is driven by the mechanical arm 6 to move above the pipette tip seat 61, the sleeve 9 of the suction and injection assembly 8 is inserted into the disposable pipette tip 78 by lowering, and the disposable pipette tip 78 is stably inserted into the sleeve 9 by continuing to lower.

[0136] The mechanical arm 6 drives the suction and injection mechanism 7 to move to the cell enrichment module 3, so that the sleeve 9 deeply inserts into the sample bottle 18, and a certain amount of sample is sucked by the disposable pipette tip 78. The mechanical arm 6 drives the suction and injection mechanism 7 to move to the film making module 5, and the sample in the disposable pipette tip 78 is injected into the film making module 5 for film making.

[0137] It can be understood that the photoelectric sensor 62 is arranged on the moving path of the transfer module 2 from the pipette tip seat 61 to the cell enrichment module 3, and is used to detect whether the disposable pipette tip 78 is installed in place.

[0138] ​Specifically, after the disposable pipette tip 78 is inserted and installed in the sleeve 9, the transfer module 2 drives the disposable pipette tip 78 to pass through the photoelectric sensor 62. The photoelectric sensor 62 detects whether the pipette tip 78 is installed in place. If the pipette tip 78 is installed in place, the transfer module 2 continues to drive the disposable pipette tip 78 to move to the cell enrichment module 3. If the pipette tip 78 is not installed in place, the transfer module 2 returns to the pipette tip module 4 to reinsert and install the pipette tip 78 until it is successfully installed, and then the transfer module 2 continues to drive the disposable pipette tip 78 to move to the cell enrichment module 3.

[0139] It can be understood that the transfer of the sample depends on the disposable pipette tip, and whether the disposable pipette tip is successfully installed directly determines whether the subsequent sample transfer is successful, and further affects whether the slide preparation and staining are successful. The present embodiment detects the step of installing the disposable pipette tip to ensure that the disposable pipette tip is successfully installed, thereby ensuring the success of the subsequent sample transfer, slide preparation and staining, and improving the success rate and production efficiency.

[0140] As a possible implementation, the pipette tip module 4 is arranged between the cell enrichment module 3 and the slide preparation module 5. In this way, the movement distance of the transfer module 2 can be reduced.

[0141] As a possible implementation, the spacing of the pipette tip site 63 is consistent with the spacing of each sleeve 9 of the pipette tip module 8. In this way, all sleeves 9 can be inserted with disposable pipette tips 78 at the same time.

[0142] Further, as shown in Figures 1-4 The slide preparation module 5 includes a workbench 64 and a slide clamp assembly 65.

[0143] The workbench 64 is composed of a plurality of workstations 66, and each workstation 66 is in the shape of a groove adapted to the slide clamp assembly 65.

[0144] The slide clamp assembly 65 includes a slide holder 67, a sedimentation bin 68 and a slide 69. A sealing rubber ring 70 is arranged between the sedimentation bin 68 and the slide 69, and the sedimentation bin 68 is connected to the slide holder 67 by a clip 71.

[0145] The slide clamp assembly is assembled as follows: the slide 69 is placed on the slide holder 67, one end of the sedimentation bin 68 with the sealing rubber ring 70 is placed on the slide 69, and the sedimentation bin 68, the slide 69 and the slide holder 67 are fixed together by the clip 71 adapted to the sedimentation bin 68 and the slide holder 67. The sedimentation bin 68 and the slide 69 form a sealed slide preparation space under the action of the sealing rubber ring 70, which is used to accommodate the sample and perform slide preparation.

[0146] The slide clamp assembly 65 can be placed in the workstation 66 and move freely. The spacing between the centers of adjacent slide workstations 66 is consistent with the spacing of each sleeve 9 of the pipette tip module 8.

[0147] The settling process is as follows: the transfer module 2 transfers the sample from the cell enrichment module 3 to the settling bin 68, and the sample is left to stand in the slide space composed of the settling bin 68, the slide 69 and the slide holder 67, and the cells are deposited on the surface of the slide 69 under the action of gravity to form a circular cell layer with the same diameter as the settling bin 68.

[0148] The slide holder assembly 65 is placed in the slide station 66, and due to the blocking of the side walls of the slide station 66 and the absence of fastening measures, the slide holder assembly 65 can move freely in the space above the slide station 66.

[0149] The suction and injection assembly 8 is driven by the mechanical arm 6 to move downward, so that the lower end of the cannula 9 enters the settling bin 68, and the lower end of the suction needle 10 stops at the corresponding position of the settling bin 68, and can be lowered to the middle position of the settling bin 68 during injection, and can be lowered to the position close to the bottom of the settling bin 68 during suction. The mechanical arm 6 drives the suction and injection assembly 8 to move horizontally to the right, and when the lower end of the suction needle 10 hits the inner wall of the settling bin 68, the settling bin 68 is subjected to the rightward pushing force from the suction needle 10, while the slide holder 67 is blocked by the right side wall of the slide station 66. The mechanical arm 6 drives the suction and injection assembly 8 to continue to move horizontally to the right, and under the continued action of the pushing force of the suction needle 10, the slide holder 67 rotates and tilts with the right end of the slide holder 67 as the axis. Due to the blocking of the front and rear side walls of the slide station 66, the slide holder 67 cannot rotate in other directions, and after the external force is removed, the slide holder 67 can return to the original position under the action of gravity. When the suction and injection assembly 8 moves horizontally to the left, front and rear, the process is the same as above.

[0150] The staining process is as follows: under the control of the controller 31, the suction and injection assembly 8 injects different reagents into the slide space through different injection needles 11 in turn, and extracts the waste liquid generated during the slide process from the slide space through the suction needle 10 and then introduces it into the waste liquid bottle 28.

[0151] During this process, the settling bin 68 is inclined during injection and suction, and is horizontal at other times. It can be understood that during injection, the reagent flows down the inclined side wall of the settling bin 68 to cover the cell layer, which can avoid direct impact of the reagent on the cell layer and cause damage to the cell layer; during suction, the inclined settling bin 68 can collect waste liquid to maximize the suction of waste liquid.

[0152] Further, as shown in Figures 1-4 the solid waste collection module 72 includes a pipe removal assembly 73 and a waste collection box 74, the pipe removal assembly 73 is arranged directly above the waste collection box 74, and the solid waste collection module 72 is used to collect the discarded disposable pipette tips 78.

[0153] The tube removing assembly 73 comprises a support plate 75 and a tube removing plate 76, the tube removing plate 76 is installed on the upper part of the support plate 75, the tube removing plate 76 is a horizontally arranged plate structure, and has an avoiding part 77. The avoiding part 77 is a U-shaped opening, the size of the opening of the avoiding part 77 is greater than the diameter of the sleeve tube 9 and less than the diameter of the top end of the disposable pipette tip 78, and the size of the avoiding part 77 gradually decreases from the opening inward, so that the sleeve tube 9 can conveniently enter the avoiding part 77, and after the sleeve tube 9 enters the avoiding part 77, the disposable pipette tip 78 can be clamped below the avoiding part 77.

[0154] The number of the tube removing assembly 73 is not less than the number of the liquid suction and injection assembly 8, so as to ensure that each liquid suction and injection assembly 8 has a corresponding tube removing assembly 73 to remove the disposable pipette tip 78 thereon. Preferably, the number of the tube removing assembly 73 is the same as the number of the liquid suction and injection assembly 8.

[0155] The distance between adjacent tube removing assemblies 73 is consistent with the distance between the sleeve tubes 9 of the liquid suction and injection assembly 8, so as to ensure that different sleeve tubes 9 enter the corresponding tube removing assembly 73 at the same time, and all the disposable pipette tips 78 on the sleeve tubes 9 are removed at the same time.

[0156] Specifically, the mechanical arm 6 drives the liquid suction and injection mechanism 7 to reach the front end of the avoiding part 77 of the tube removing assembly 73, and makes the sleeve tube 9 of the liquid suction and injection assembly 8 enter the inside of the tube removing plate 76 from the avoiding part 77. The mechanical arm 6 drives the liquid suction and injection mechanism 7 to move upward, so that the upper end of the disposable pipette tip 78 is blocked by the tube removing plate 76 and cannot continue to move upward with the liquid suction and injection mechanism 7, thereby falling off from the sleeve tube 9 and falling into the waste collection box 74 directly below the tube removing assembly 73.

[0157] On the other hand, a preparation method of a cell slide is also provided, which is prepared by using the automatic liquid-based cell preparation machine, and comprises the following steps:

[0158] S1, sample enrichment: first, the centrifugal action of the cell enrichment module 3 is used to deposit the cells and other diagnostic components at the bottom tip of the sample bottle 18; then the supernatant after centrifugation is poured away by the waste liquid pouring device 14; finally, the reagent is injected into the cell sediment by the liquid suction and injection mechanism 7 of the transfer module 2, and the sample is mixed by suction.

[0159] S2, sample transfer: a certain amount of mixed sample is sucked into the disposable pipette tip 78 by the liquid suction and injection mechanism 7 of the transfer module 2, and the sample is transferred to the sedimentation bin 68 of the preparation module 5.

[0160] S3, sedimentation and preparation: the sample in the sedimentation bin 68 is allowed to stand for 5-20 minutes, and the cells and other diagnostic components in the sample naturally sediment on the slide 69 under the action of gravity.

[0161] S4, dyeing: the suction and injection mechanism 7 of the transport module 2 injects and sucks out different reagents such as dyeing solution, alcohol, water, buffer solution and transparent solution into and out of the sedimentation bin 68 of the slice preparation module 5 for multiple times, to complete the dyeing and transparency of the cells.

[0162] It is to be noted that the terms "comprising", "containing", or any other variant thereof are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can also include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without more limitations, an element defined by the phrase "comprising a" does not exclude the existence of additional identical elements in the process, method, article, or apparatus that includes the element.

[0163] The embodiments of the utility model are described above in combination with the drawings, but the utility model is not limited to the specific embodiments described above, and the specific embodiments described above are only illustrative but not restrictive, and those skilled in the art can make many forms under the inspiration of the utility model without departing from the scope of the utility model and the scope protected by the claims, which all belong to the protection of the utility model.

Claims

1. An automatic trim device characterized by comprising: The centrifugal turntable, the detection unit, the transfer module, and several balancing blocks are included. The centrifugal turntable is uniformly provided with sample bottle bearing seats on the outer periphery. The detection unit detects whether the sample bottles in the centrifugal turntable are balanced, and records the sample bottle bearing seats that need to be balanced. The transfer module includes a mechanical arm and a transfer suction disc, and the transfer suction disc is fixedly installed on the mechanical arm.

2. The automatic trim device of claim 1, wherein The mechanical arm includes an X arm, a Y arm, and a Z arm. The X arm is movably installed on one side of the tablet preparation module, the Y arm is movably installed on the X arm, and the Z arm is movably installed on the Y arm.

3. The automatic trim device of claim 1, wherein The detection unit includes a positioning sensor and a bottle detection sensor. The positioning sensor includes assembly one and assembly two, assembly one is fixedly arranged on the centrifugal turntable and rotates with the centrifugal turntable, assembly one and assembly two are used in cooperation to position the sample bottle bearing seat. The bottle detection sensor is arranged on one side of the centrifugal turntable and is slightly higher than the sample bottle bearing seat, and the bottle detection sensor determines whether there is a sample bottle in the sample bottle bearing seat. The positioning sensor and the bottle detection sensor cooperate to confirm the sample bottle bearing seat that needs to place the balancing block.

4. The automatic trim device of claim 1, wherein The balancing block is a columnar structure with the same weight as the sample bottle, has a non-through insertion hole in the middle, and the insertion hole diameter is slightly larger than the sleeve of the liquid suction and injection assembly.

5. The automatic trim device of claim 1, wherein The balancing block is a columnar structure with the same weight as three sample bottles, has a non-through insertion hole in the middle, and the insertion hole diameter is slightly larger than the sleeve of the liquid suction and injection assembly.

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