Outdoor portable root-knot nematode separating device

Abstract

The utility model belongs to the field of root-knot nematode separation, and particularly relates to an outdoor portable root-knot nematode separation device which comprises a metal box body with an upper cover and a transparent inner shell which is placed in the metal box body, and the outer wall of the transparent inner shell is tightly attached to the inner wall of the metal box body. The metal box body comprises an outer shell and an outer cover; a partition plate perpendicular to the bottom wall of the metal box body is arranged in the inner shell; the inner shell is divided into a plurality of mutually independent partitions by the partition plates; a water outlet penetrating through the inner shell and the metal box body is formed in the bottom wall of each subarea; a metal plate which is parallel to the bottom of the box body and is provided with a round hole is arranged in the middle of the interior of each subarea; each metal plate is fixedly provided with a short-neck funnel, and the lower end of each short-neck funnel penetrates through the corresponding round hole; and the bottom of the funnel neck of the funnel is connected with the water outlet. The separation device not only can ensure the sample quality, but also can save the treatment time and improve the separation efficiency and accuracy, thereby improving the reliability of soil nematode detection.

Description

Technical Field

[0001] This utility model belongs to the field of root-knot nematode isolation, specifically relating to an outdoor portable root-knot nematode isolation device. Background Technology

[0002] Root-knot nematodes are important plant pathogenic nematodes widely distributed worldwide, parasitizing and damaging various food and horticultural crops, causing significant economic losses. Determining the number of root-knot nematodes in the soil is crucial for their control. Current nematode isolation devices, such as the Buchner funnel, primarily rely on the nematodes' negative phototaxis and gravity, allowing them to sink naturally and separate from the soil. However, only one soil sample can be isolated at a time. This method suffers from long processing times, cumbersome procedures, and limitations in timely laboratory processing after field sampling, which may lead to nematode mortality and affect the reliability of the final test results. Summary of the Invention

[0003] This invention addresses the problems of long processing cycles, cumbersome operations, and the inability to promptly process samples in the laboratory after field sampling in current root-knot nematode isolation methods, by providing a portable outdoor root-knot nematode isolation device.

[0004] The technical solution claimed by this utility model is as follows:

[0005] An outdoor portable root-knot nematode separation device includes a metal box 1 with an open top and a transparent inner shell 2 placed inside the metal box 1 with its outer wall tightly attached to the inner wall of the metal box 1. The metal box 1 includes an outer shell 3 and an outer cover 4. A partition 5 is arranged perpendicular to the bottom wall of the metal box 1 inside the inner shell 2. The partition 5 divides the inner shell 2 into multiple independent partitions 6. Each partition 6 has a drain outlet 7 that passes through the inner shell 2 and the metal box 1 on its bottom wall. A metal plate 8 with a circular hole is arranged parallel to the bottom of the box and in the middle of each partition 6. The metal plate 8 divides the partition 6 into an upper cavity and a lower cavity. Each metal plate 8 is fixedly provided with a short-necked funnel 9 whose lower end passes through the circular hole. The bottom of the neck of the funnel 9 is connected to the drain outlet.

[0006] Preferably, the inner shell 2 is made of transparent plastic material.

[0007] Preferably, the funnel 9 is made of glass.

[0008] Preferably, an inner cover 10 is provided above the partition 6.

[0009] Preferably, a replaceable gauze is suspended in the upper cavity of the partition 6.

[0010] Preferably, the diameter of the circular hole on the metal plate 8 is 90 mm.

[0011] Preferably, the metal plate 8 has a threaded groove on the edge of the circular hole, and the funnel 9 has a thread on the outer wall of the neck corresponding to the threaded groove.

[0012] Preferably, the metal box 1 is a cuboid.

[0013] Preferably, the outer walls of the outer casing 3 are welded with perforated bolts.

[0014] Preferably, the metal box 1 has a length of 300-400mm, a width of 200-250mm, and a height of 200-300mm; the funnel 9 has an opening diameter of 120mm, a neck outer diameter of 13-15mm, a neck wall thickness of 1.5-2.5mm, a neck length of 120mm, and a filter bowl thickness of 1.5-3.5mm.

[0015] Beneficial effects:

[0016] This invention provides a portable outdoor root-knot nematode isolation device, comprising a metal box 1 with an open top and a transparent inner shell 2 placed inside the metal box 1 with its outer wall tightly attached to the inner wall of the metal box 1; a partition 5 is arranged perpendicularly to the bottom wall of the metal box 1 inside the inner shell 2; the partition 5 divides the inner shell 2 into multiple independent partitions 6. Each partition 6 is independent of the others. When root-knot nematodes need to be isolated, simply place soil wrapped in gauze into the funnel 9 of partition 6 and add an appropriate amount of distilled water to achieve the separation of root-knot nematodes from the soil. Due to the multiple partitions 6, root-knot nematodes can be separated from multiple soil samples simultaneously, solving the problems of long processing cycles and cumbersome operations in current root-knot nematode isolation methods. In addition, the root-knot nematode isolation device has a simple structure, is easy to carry, and can be taken directly outdoors to complete soil sample collection and root-knot nematode isolation outdoors, solving the problem that current root-knot nematode isolation is limited by the inability to perform timely laboratory processing after field sampling. Therefore, the separation device can ensure sample quality, save processing time, improve separation efficiency and accuracy, thereby improving the reliability of soil nematode detection.

[0017] The inner shell 2 is made of transparent plastic material, which allows researchers to directly observe the number of isolated root-knot nematodes.

[0018] An inner cover 10 is provided above the partition 6 to prevent the partitions from affecting each other and causing inaccurate data.

[0019] The upper cavity of partition 6 is equipped with replaceable gauze, allowing researchers to directly wrap soil samples in the gauze for separation.

[0020] The metal plate 8 has a threaded groove on the edge of the circular hole, and the funnel 9 has a thread on the outer wall of the neck that corresponds to the threaded groove, so as to improve the stability of the funnel.

[0021] The outer shell 3 has symmetrical bolts welded on its outer wall. When going out, you can simply pass the rope through the two bolts in sequence and tighten it to carry it by hand.

[0022] In summary, the root-knot nematode separation device provided by this utility model has the following advantages: (1) Improved efficiency: By setting up the device once, root-knot nematode separation can be carried out immediately outdoors, reducing the time from sampling to laboratory processing and speeding up the entire detection process; (2) Guaranteed sample quality: The device has a reasonable internal design, which can effectively protect soil samples from the influence of the external environment, avoid sample loss or changes, and ensure the accuracy of detection results; (3) Simplified operation: The device has a clear and simple structure, is easy to operate, does not require complicated steps and equipment, reduces the difficulty of operation, and improves the efficiency of use; (4) Cost saving: It reduces manual operation time and possible errors, saves manpower and material resources, reduces detection costs, and improves laboratory efficiency. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the outdoor portable root-knot nematode separation device in the open state according to an embodiment of this utility model.

[0024] Figure 2 This is a front view of the outdoor portable root-knot nematode separation device in the open state according to an embodiment of this utility model.

[0025] Figure 3 This is a schematic diagram of the open-top state of the inner shell of the outdoor portable root-knot nematode separation device according to an embodiment of this utility model.

[0026] In the diagram: 1. Metal box; 2. Inner shell; 3. Outer shell; 4. Outer cover; 5. Partition; 6. Zone; 7. Drain outlet; 8. Metal plate; 9. Funnel; 10. Inner cover. Detailed Implementation

[0027] The present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are for explanation and illustration only and do not limit the scope of the present invention in any way.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0029] This invention provides an outdoor portable root-knot nematode separation device, such as... Figure 1 As shown, it includes a metal box 1 with an open top and a transparent inner shell 2 placed inside the metal box 1 with its outer wall tightly attached to the inner wall of the metal box 1; in a specific embodiment of the present invention, the inner shell 2 is made of transparent plastic.

[0030] The metal box 1 includes an outer shell 3 and an outer cover 4; the outer shell 3 has symmetrical bolt holes welded on its outer wall to facilitate the carrying of the separation device.

[0031] An inner cover 10 is provided above the partition 6; a partition 5 is provided inside the inner shell 2 perpendicular to the bottom wall of the metal box 1; the partition 5 divides the inner shell 2 into multiple independent partitions 6; a drain outlet 7 is provided on the bottom wall of each partition 6, penetrating the inner shell 2 and the metal box 1; a metal plate 8 is provided in the middle of the interior of each partition 6, parallel to the bottom of the box and with a circular hole, the diameter of the circular hole on the metal plate 8 being 90mm; the metal plate 8 divides the partition 6 into an upper cavity and a lower cavity, and a replaceable gauze is suspended in the upper cavity; a short-necked funnel 9 with its lower end passing through the circular hole is fixedly provided on each metal plate 8; the bottom of the neck of the funnel 9 is connected to the drain outlet.

[0032] In a specific embodiment of this utility model, the metal box 1 is a cuboid; the length of the metal box 1 is 300-400mm, the width is 200-250mm, and the height is 200-300mm; the funnel 9 has an opening diameter of 120mm, an outer diameter of the neck of 13-15mm, a neck wall thickness of 1.5-2.5mm, a neck length of 120mm, and a filter bowl thickness of 1.5-3.5mm.

[0033] In a specific embodiment of this utility model, the specific method of using the separation device is as follows: take a rope of appropriate length, pass it through the two bolts in sequence and tie it tightly, carry the rope and take the separation device outdoors. When sampling outdoors, wrap the soil sample with gauze and put it into the funnel 9 of the partition 6, and add an appropriate amount of distilled water. The device will automatically separate the root-knot nematodes. Each partition will not interfere with the others. Different soil samples will be placed in different partitions. After a period of time, check the number of root-knot nematodes in the lower cavity. Specifically, the inner shell 2 is placed in the metal box 1. If it is necessary to count the number of root-knot nematodes in each partition, simply take out the inner shell 2 from the metal box 1 and directly observe the number of root-knot nematodes in the lower cavity of each partition.

[0034] The above are merely specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this utility model, and these modifications or substitutions should all be covered within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. An outdoor portable root-knot nematode separation device, characterized in that, The device includes a metal box (1) with an open top and a transparent inner shell (2) placed inside the metal box (1) with its outer wall tightly attached to the inner wall of the metal box (1); the metal box (1) includes an outer shell (3) and an outer cover (4); a partition (5) is provided inside the inner shell (2) perpendicular to the bottom wall of the metal box (1); the partition (5) divides the inner shell (2) into multiple independent partitions (6); each partition (6) has a drain outlet (7) that passes through the inner shell (2) and the metal box (1) on its bottom wall; each partition (6) has a metal plate (8) with a hole in the middle, parallel to the bottom of the box, which divides the partition (6) into an upper cavity and a lower cavity; each metal plate (8) has a short-necked funnel (9) with its lower end passing through the hole; the bottom of the neck of the funnel (9) is connected to the drain outlet.

2. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, The inner shell (2) is made of transparent plastic.

3. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, The funnel (9) is made of glass.

4. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, An inner cover (10) is provided above the partition (6).

5. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, Replaceable gauze is suspended in the upper cavity of the partition (6).

6. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, The diameter of the circular hole on the metal plate (8) is 90 mm.

7. The portable outdoor root-knot nematode separation device according to claim 6, characterized in that, The metal plate (8) has a threaded groove on the edge of the circular hole, and the funnel (9) has a thread on the outer wall of the neck that corresponds to the threaded groove.

8. The portable outdoor root-knot nematode separation device according to claim 1, characterized in that, The metal box (1) is a cuboid.

9. The portable outdoor root-knot nematode separation device according to claim 6, characterized in that, The outer wall of the outer shell (3) is welded with perforated bolts.

10. The portable outdoor root-knot nematode separation device according to claim 6, characterized in that, The metal box (1) has a length of 300-400mm, a width of 200-250mm, and a height of 200-300mm; the funnel (9) has an opening diameter of 120mm, an outer diameter of the neck of 13-15mm, a neck wall thickness of 1.5-2.5mm, a neck length of 120mm, and a filter bowl thickness of 1.5-3.5mm.

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