Protein purification pretreatment device

Abstract

The utility model relates to the field of protein purification, and is characterized in that the utility model discloses a protein purification pretreatment device which comprises a barrel body, the top of the barrel body 1 is fixedly connected with a bracket, the surface of the bracket is fixedly connected with an air cylinder, the output end of the air cylinder is fixedly connected with a motor through a mounting frame, and the output end of the motor is fixedly connected with a rotating shaft; a grinding disc is arranged at the bottom of the rotating shaft, a cleaning structure is arranged on the surface of the grinding disc and comprises a scraping plate, water outlet holes are formed in the surface of the scraping plate in a penetrating mode, a fixing frame is fixedly connected to the upper surface of the grinding disc, and a water conveying assembly is arranged on the surface of the scraping plate. And a dismounting and mounting assembly is arranged in the fixing frame, the water conveying assembly comprises a threaded hole, and the inner wall of the threaded hole is in threaded connection with a screw pipe. According to the utility model, the cleaning structure is arranged, so that residues attached in the cylinder body are prevented from being inconvenient to clean, and the subsequent protein purification pretreatment effect of the cylinder body is ensured.

Description

Technical Field

[0001] This utility model relates to the field of protein purification, and in particular to a protein purification pretreatment device. Background Technology

[0002] Protein isolation and purification is an important biological manipulation technique. Protein purification is widely used in fields such as biochemical research and drug development. The general steps of protein isolation and purification can be divided into three parts: pretreatment, crude separation, and fine separation. During the pretreatment process of protein purification, the material needs to be stirred and ground to improve the efficiency of subsequent purification.

[0003] A search revealed a Chinese patent publication number: CN219210123U, which discloses a protein purification pretreatment device. The device performs grinding by moving the grinding disc up and down or rotating it, allowing for grinding and stirring of protein materials on the same device, making it highly practical.

[0004] In the above technical solution, the protein material is ground by moving up and down and rotating the grinding disc. However, after the grinding operation is completed, some residual protein material will adhere to the inner wall of the cylinder, which is not easy to clean, thus reducing the efficiency of protein purification. Therefore, a protein purification pretreatment device is proposed to solve the above problem. Utility Model Content

[0005] To overcome the above deficiencies, this utility model provides a protein purification pretreatment device, which aims to improve the problem that the lack of a cleaning function for the inner wall of the cylinder in the existing protein purification pretreatment device leads to protein material adhesion affecting subsequent grinding operations and reducing the efficiency of protein purification pretreatment.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a protein purification pretreatment device, comprising a cylindrical body, a support fixedly connected to the top of the cylindrical body 1, a cylinder fixedly connected to the surface of the support, a motor fixedly connected to the output end of the cylinder via a mounting frame, a rotating shaft fixedly connected to the output end of the motor, a grinding disc provided at the bottom of the rotating shaft, a cleaning structure provided on the surface of the grinding disc, the cleaning structure including a scraper, a water outlet hole penetrating the surface of the scraper, a fixing frame fixedly connected to the upper surface of the grinding disc, a water conveying component provided on the surface of the scraper, and a disassembly and assembly component provided inside the fixing frame.

[0007] Furthermore, a positioning hole is provided at the end of the rotating shaft away from the motor, a through hole is provided on the surface of the rotating shaft, a support column is fixedly connected to the top of the grinding disc, and a threaded column is threadedly connected to the inner wall of the support column.

[0008] Preferably, the water conveying assembly includes a threaded hole, the inner wall of which is threaded with a spiral tube, the outer wall of which is threaded with a telescopic tube via a collar, the port of which is fixedly connected to a water pump, and the inner wall of the threaded hole is provided with a sealing plug.

[0009] Preferably, the disassembly and assembly assembly includes a T-slot, a T-block is disposed inside the T-slot, and a support plate is fixedly connected to the top of the T-block.

[0010] Preferably, the threaded hole is formed on the inner wall of the grinding disc, the outer wall of the spiral tube is fixedly connected to the inner wall of the scraper, and the outer wall of the sealing plug is adapted to the inner wall of the threaded hole.

[0011] In addition, the T-slot is formed on the inner surface of the fixed frame; the outer wall of the upper end of the support column is adapted to the inner wall of the positioning hole; and the inner wall of the through hole is threadedly connected to the outer wall of the threaded column.

[0012] By implementing the above technical solution, this utility model can achieve the following beneficial technical effects:

[0013] 1. This utility model allows for quick assembly and disassembly of the scraper and grinding disc by using a threaded hole and a screw tube. The T-shaped block and T-slot fix the water pump in the fixed frame. The connection between the telescopic tube and the screw tube allows water to be introduced into the scraper and then sprayed out through the water outlet. This allows the scraper to follow the rotation of the grinding disc to scrape and wash away the protein material attached to the cylinder wall, without affecting the normal function of the grinding disc to stir and grind the protein material. This avoids residues in the cylinder that are difficult to clean and ensures the effectiveness of the cylinder in the subsequent protein purification pretreatment.

[0014] 2. In this utility model, the positioning hole facilitates the insertion of the support column and its connection with the rotating shaft. The threaded column and the through hole can fix the support column and the rotating shaft, thereby facilitating the installation and removal of the entire grinding disc from the cylinder. It also facilitates the cleaning of the elastically connected protrusions on the grinding disc, reduces cleaning dead angles, and ensures the cleanliness of subsequent grinding operations. Attached Figure Description

[0015] Figure 1 This is a side view of the main structure of a protein purification pretreatment device proposed in this utility model;

[0016] Figure 2 This is a top view schematic diagram of the separation of local structures in the removal cylinder of a protein purification pretreatment device proposed in this utility model;

[0017] Figure 3 This is a side view of the separation of a partial structure of the cylindrical body in a protein purification pretreatment device proposed in this utility model;

[0018] Figure 4This is a bottom view of a partial structure of a protein purification pretreatment device proposed in this utility model;

[0019] Figure 5 This is a top view schematic diagram of a partial structure of a protein purification pretreatment device proposed in this utility model.

[0020] Legend:

[0021] 1. Cylinder; 2. Support; 3. Cylinder; 4. Motor; 5. Shaft; 6. Grinding disc; 7. Threaded hole; 8. Spiral tube; 9. Scraper; 10. Water outlet; 11. Fixing frame; 12. T-slot; 13. T-block; 14. Support plate; 15. Telescopic tube; 16. Sealing plug; 17. Water pump; 18. Positioning hole; 19. Support column; 20. Through hole; 21. Threaded column. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Reference Figures 1-3This utility model provides an embodiment of a protein purification pretreatment device, comprising a cylindrical body 1, a support 2 fixedly connected to the top of the cylindrical body 1, and a cylinder 3 fixedly connected to the surface of the support 2. The cylinder 3, through a mounting frame, can drive a grinding disc 6 to move up and down inside the cylindrical body 1. After the cylindrical body 1 completes one protein purification pretreatment, its interior needs to be cleaned. The cylinder 3 is activated to drive the grinding disc 6 to rise. The output end of the cylinder 3 is fixedly connected to a motor 4 through the mounting frame, and the output end of the motor 4 is fixedly connected to a rotating shaft 5. The bottom of the rotating shaft 5 is provided with a grinding disc 6. The bottom of the grinding disc 6 is provided with several sets of equidistantly distributed protrusions, and the protrusions are elastically connected to the bottom of the grinding disc 6. The surface of the grinding disc 6 is provided with a cleaning structure, including a scraper 9. Insert the screw tube 8 into the threaded hole 7 and rotate it clockwise until the scraper 9 is fixed on the lower surface of the grinding disc 6. The scraper 9 is arranged in a trapezoidal triangular shape to facilitate scraping off the protein material attached to the wall of the cylinder 1. A water outlet hole 10 is opened through the surface of the scraper 9. A fixing frame 11 is fixedly connected to the upper surface of the grinding disc 6. The fixing frame 11 is used to install the water pump 17. A water conveying component is provided on the surface of the scraper 9. A disassembly and assembly component is provided inside the fixing frame 11. The cylinder 1, cylinder 3, motor 4, grinding disc 6 and protruding rod mentioned above are similar in principle to the cylinder, cylinder, motor, grinding disc and protruding rod mentioned in Chinese Patent No. CN219210123U (A Protein Purification Pretreatment Device), and achieve the same effect. They are existing public technical means in this field, and will not be described in detail here.

[0024] Therefore, in addition to the aforementioned technical effects, this utility model also has the following technical effects:

[0025] (1) By setting up the fit between the screw tube and the threaded hole, as well as the assembly and disassembly components of the T-block and T-slot, the disassembly, installation and maintenance of the equipment become simpler and faster. This greatly reduces the maintenance difficulty for operators and improves work efficiency.

[0026] (2) The quick disassembly design of the grinding disc and the cylinder makes the cleaning and maintenance process easier, reduces downtime, and improves the overall utilization rate of the equipment.

[0027] (3) The combined design of the scraper and the water outlet allows the cleaning fluid to be evenly distributed on the inner wall of the cylinder, effectively scraping off and rinsing the protein material adhering to the cylinder wall. This not only improves the cleaning effect but also reduces cleaning time and water consumption, saving resources.

[0028] (4) The water delivery assembly consisting of a water pump and a telescopic pipe can precisely control the flow rate and direction of the cleaning fluid, further optimizing the cleaning effect.

[0029] (5) The elastic protrusions at the bottom of the grinding disc can adapt to protein materials with different hardness and viscosity, thus improving the adaptability of the equipment.

[0030] (6) The quick-change design of the grinding disc and scraper enables the equipment to be quickly adjusted according to different processing needs, enhancing the flexibility of the equipment.

[0031] (7) Key components such as scrapers and grinding discs are made of wear-resistant and corrosion-resistant materials, which can resist the erosion of cleaning fluids and protein materials, thus improving the durability of the equipment.

[0032] Reference Figures 3-5 The water supply component includes a threaded hole 7. A helical tube 8 is aligned with the threaded hole 7 and rotated clockwise to connect them. After connection, the top of the helical tube 8 protrudes slightly on the grinding disc 6. The inner wall of the threaded hole 7 is threaded with the helical tube 8, and the outer wall of the helical tube 8 is threaded with a telescopic tube 15 via a collar thread. A water pump 17 is fixedly connected to the end of the telescopic tube 15. A sealing plug 16 is provided on the inner wall of the threaded hole 7. The sealing plug 16 can seal the helical tube 8 after it is disengaged from the threaded hole 7, ensuring that the grinding disc 6 does not interfere with the grinding of protein materials. The disassembly component includes a T-slot 12, inside which is a T-block 13. A support plate 14 is fixedly connected to the top of the T-block 13. The input end of the water pump 17 is connected to an external water pipe to introduce water. The telescopic tube 15 is pulled and aligned with the helical tube 8, rotating to connect the helical tube 8 and the telescopic tube 15. The threaded hole 7 is located on the inner wall of the grinding disc 6. The outer wall of the spiral tube 8 is fixedly connected to the inner wall of the scraper 9. The cavity inside the spiral tube 8 and the scraper 9 is connected. The outer wall of the sealing plug 16 is adapted to the inner wall of the threaded hole 7. The T-slot 12 is opened on the inner surface of the fixed frame 11. After the T-block 13 is inserted into the T-slot 12, the support plate 14 magnetically attracts the inner surface of the fixed frame 11 to ensure the stability of the water pump 17. The cylinder 3 is restarted to drive the grinding disc 6 to descend into the interior of the cylinder 1. The motor 4 is turned on to drive the rotating shaft 5 to rotate in both directions. The scraper 9 is close to the cylinder wall of the cylinder 1. The water pump 17 discharges water into the interior of the scraper 9 through the telescopic pipe 15. Finally, the water is sprayed out through several sets of water outlet holes 10 to rinse and scrape off the protein material attached to the inner wall of the cylinder 1, ensuring the cleaning effect of the cylinder 1. Finally, the above steps are reversed to remove the scraper 9 and the water pump 17. This will not affect the subsequent grinding of the protein material by the cylinder 1.

[0033] Therefore, in conjunction with the above technical solutions, the protein purification pretreatment device of this utility model can also achieve the following technical effects:

[0034] (1) By setting the sealing plug 16, when the screw tube 8 is disengaged from the threaded hole 7, the sealing plug 16 can automatically seal the threaded hole 7. This design effectively prevents the cleaning fluid or other liquids from leaking from the threaded hole 7, ensuring the safety and cleanliness of the equipment.

[0035] (2) The T-slot 12 and T-block 13 of the disassembly and assembly components are designed to magnetically fix the inner surface of the fixed frame 11 with the support plate 14, so that the water pump 17 can be stably fixed in the fixed frame 11 during the water delivery process and will not be displaced or fall off due to vibration or other factors, thereby improving the safety and stability of the equipment.

[0036] (3) The water supply assembly consisting of water pump 17 and telescopic pipe 15 can precisely control the flow rate and direction of the cleaning liquid, ensuring that the cleaning liquid can be evenly sprayed on scraper 9 and evenly sprayed out through water outlet 10, thereby optimizing the cleaning effect on the inner wall of cylinder 1 and improving cleaning efficiency.

[0037] (4) The water supply component and disassembly component of this utility model are designed in a simple and clear manner, and the operation process is simple and easy to understand. Operators only need to follow the steps to easily complete the installation, disassembly and supply of cleaning fluid to the water pump 17, which reduces the difficulty of operation and improves work efficiency.

[0038] Reference Figures 4-5 A positioning hole 18 is provided at the end of the rotating shaft 5 away from the motor 4. A through hole 20 is provided through the surface of the rotating shaft 5. The through hole 20 facilitates the insertion of the threaded post 21 to connect the support post 19 to the rotating shaft 5. The threaded post 21 is reversed so that it gradually disengages from the two sets of through holes 20 and the support post 19. The top of the grinding disc 6 is fixedly connected to the support post 19. The inner wall of the support post 19 is threadedly connected to the threaded post 21. The outer wall of the upper end of the support post 19 is adapted to the inner wall of the positioning hole 18. After the support post 19 is disengaged from the rotating shaft 5, the grinding disc 6 can be quickly disassembled, which is convenient for cleaning the multiple sets of protrusions at the bottom of the grinding disc 6. The inner wall of the through hole 20 is threadedly connected to the outer wall of the threaded post 21. At this time, the weight of the grinding disc 6 will drive the support post 19 to disengage from the rotating shaft 5, which is convenient for disassembling the grinding disc 6 as a whole and cleaning the multiple sets of protrusions of the grinding disc 6. This ensures the cleanliness of the cylinder 1 and the grinding and stirring device, and ensures the effect of subsequent protein purification pretreatment.

[0039] Therefore, based on the above Figures 4-5 The technical solution described above, the protein purification pretreatment device has the following technical effects:

[0040] (1) By setting the positioning hole 18 and the support column 19, the grinding disc 6 can be quickly removed from the rotating shaft 5. This greatly simplifies the cleaning process, making it easier for operators to thoroughly clean the multiple sets of protrusions at the bottom of the grinding disc 6, thereby improving cleaning efficiency.

[0041] (2) The quick-release design of the grinding disc 6 makes maintenance and replacement easier. If the grinding disc 6 or the protrusions on it wear or are damaged due to prolonged use, the operator can quickly disassemble and replace it with a new grinding disc, thereby reducing the maintenance cost of the equipment.

[0042] (3) The design of the threaded column 21 and the through hole 20 makes the connection between the support column 19 and the rotating shaft 5 both firm and easy to disassemble. This design allows the equipment to be operated quickly when maintenance or replacement of parts is required, thus enhancing the maintainability of the equipment.

[0043] (4) The quick disassembly and cleaning of the grinding disc 6 ensures that it is in optimal condition before each use. This helps to optimize the performance of the equipment and improve the efficiency and quality of protein purification pretreatment.

[0044] (5) By providing positioning holes 18 and support columns 19, operators can safely disassemble and install the grinding disc 6 without contacting the motor or other moving parts. This reduces safety risks during operation and improves operator safety.

[0045] (6) The quick-release design of the grinding disc 6 allows the equipment to adapt to different grinding needs. If it is necessary to replace different types of grinding discs (such as grinding discs of different sizes and shapes), the operator can quickly disassemble the old grinding disc and install the new grinding disc, thereby enhancing the versatility and flexibility of the equipment.

[0046] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A protein purification pretreatment device, comprising a cylindrical body (1), a support (2) fixedly connected to the top of the cylindrical body (1), a cylinder (3) fixedly connected to the surface of the support (2), a motor (4) fixedly connected to the output end of the cylinder (3) via a mounting frame, a rotating shaft (5) fixedly connected to the output end of the motor (4), and a grinding disc (6) provided at the bottom of the rotating shaft (5), characterized in that: The surface of the grinding disc (6) is provided with a cleaning structure, which includes a scraper (9). A water outlet hole (10) is opened through the surface of the scraper (9). A fixing frame (11) is fixedly connected to the upper surface of the grinding disc (6). A water conveying component is provided on the surface of the scraper (9). A disassembly and assembly component is provided inside the fixing frame (11).

2. The protein purification pretreatment apparatus according to claim 1, characterized in that: The rotating shaft (5) has a positioning hole (18) at one end away from the motor (4), and a through hole (20) is opened through the surface of the rotating shaft (5). A support column (19) is fixedly connected to the top of the grinding disc (6), and a threaded column (21) is threadedly connected to the inner wall of the support column (19).

3. The protein purification pretreatment apparatus according to claim 1, characterized in that: The water delivery assembly includes a threaded hole (7), the inner wall of the threaded hole (7) is threaded with a helical tube (8), the outer wall of the helical tube (8) is threaded with a telescopic tube (15) through a collar, the port of the telescopic tube (15) is fixedly connected to a water pump (17), and the inner wall of the threaded hole (7) is provided with a sealing plug (16).

4. The protein purification pretreatment apparatus according to claim 1, characterized in that: The assembly and disassembly assembly includes a T-slot (12), inside which a T-block (13) is provided, and a support plate (14) is fixedly connected to the top of the T-block (13).

5. The protein purification pretreatment apparatus according to claim 3, characterized in that: The threaded hole (7) is opened on the inner wall of the grinding disc (6), the outer wall of the spiral tube (8) is fixedly connected to the inner wall of the scraper (9), and the outer wall of the sealing plug (16) is adapted to the inner wall of the threaded hole (7).

6. The protein purification pretreatment apparatus according to claim 4, characterized in that: The T-slot (12) is formed on the inner surface of the fixed frame (11).

7. The protein purification pretreatment apparatus according to claim 2, characterized in that: The outer wall of the upper end of the support column (19) is adapted to the inner wall of the positioning hole (18).

8. The protein purification pretreatment apparatus according to claim 2, characterized in that: The inner wall of the through hole (20) is threadedly connected to the outer wall of the threaded column (21).

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