Fluorescein labeled antibody device

Abstract

The utility model relates to the technical field of fluorescein labeled antibodies, in particular to a fluorescein labeled antibody device which comprises a mounting part, a cavity is formed in the mounting part, a piston is slidably connected to the inner wall of the cavity, a threaded cylinder is fixedly connected to the inner wall of the piston, and the cavity is formed in the mounting part. A piston is placed on the inner wall of the cavity, a liquid inlet pipe and a liquid conveying pipe are arranged, fluorescein can be conveniently input into the cavity through the liquid inlet pipe, then a threaded plug is rotated for sealing, a threaded rod is rotated to be connected with a threaded barrel, and a guide rod and the piston are limited, so that the piston can descend under the action of the threaded barrel; according to the device, the threaded barrel, the piston and the threaded rod are arranged for connection, so that the fluorescein can be conveniently added to the lower portion for reaction, the whole operation process is simplified, the operation procedures of fluorescein injection, antibody injection, film tearing and the like are not needed, and use is convenient.

Description

Technical Field

[0001] This application relates to the technical field of fluorescently labeled antibodies, and in particular to a fluorescently labeled antibody device. Background Technology

[0002] Antibody labeling refers to the covalent attachment of a marker to an antibody, which reacts specifically with the analyte to form a multi-component complex. The test results can be directly observed under a microscope or automatically measured using sophisticated instruments such as fluorescence microscopes, X-ray measuring instruments, enzyme-linked immunosorbent assay (ELISA) instruments, electron microscopes, flow cytometers, and chemiluminescence immunoassay analyzers. It can be used to conduct qualitative and localization studies of antigen-antibody reactions at the cellular, subcellular, ultrastructural, and molecular levels, or to qualitatively and quantitatively determine haptens and antigens in body fluids using various liquid-phase and solid-phase immunoassay methods.

[0003] A search revealed Chinese Patent Publication No. CN211505557U, which discloses a fluorescein-labeled antibody device. The device includes an outer tube into which an inner tube is inserted. A labeling device is connected to the bottom of the inner tube. The labeling device includes a hollow tubular ultrafiltration membrane connected to the bottom of the inner tube. A reaction dish is connected to the bottom of the ultrafiltration membrane, and the reaction dish contains fluorescein. The reaction dish is sealed with a protective membrane. This invention allows unreacted fluorescein to be removed through the ultrafiltration membrane via centrifugation, leaving the antibody in the reaction dish as the fluorescein-labeled antibody. The entire labeling process is convenient, quick, and simple to operate, saving manpower and time and improving work efficiency.

[0004] Regarding the aforementioned technologies, the inventors have discovered the following drawbacks: In the above-mentioned solutions, it is found that it is necessary to first inject the fluorescein into the reaction dish, and then inject the antibody through the protective membrane using a syringe. After the reaction is completed, the protective membrane is then removed. The operation steps are cumbersome and inconvenient to use. Utility Model Content

[0005] To make the operation of fluorescently labeled antibodies simpler and faster, and to eliminate cumbersome operating steps, this application provides a fluorescently labeled antibody device.

[0006] This application provides a fluorescently labeled antibody device, which adopts the following technical solution: It includes an installation component with a cavity inside. A piston is slidably connected to the inner wall of the cavity. A threaded cylinder is fixedly connected to the inner wall of the piston. A threaded rod is threadedly connected to the inner wall of the threaded cylinder. The outer surface of the threaded rod is rotatably connected to the inner wall of the installation component. An inlet pipe and an infusion pipe are fixedly installed on the inner wall of the installation component. The bottom end of the inlet pipe and the top end of the infusion pipe are both fixedly connected to the inner wall of the cavity. A threaded plug is threadedly connected to the upper surface of the installation component. Two guide rods are fixedly connected to the inner bottom wall of the cavity, and the outer surface of each guide rod is slidably connected to the inner wall of the piston.

[0007] Optionally, the bottom surface of the mounting component has two sliding grooves, and a sliding block is slidably connected to the inner wall of each sliding groove.

[0008] Optionally, each of the sliding blocks has a spring and a connecting rod fixedly connected to its bottom surface, the bottom end of each spring is fixedly connected to the inner bottom wall of the corresponding sliding groove, and the outer surface of each connecting rod is slidably connected to the inner wall of the corresponding sliding groove.

[0009] Optionally, a connecting frame is provided below the mounting component, and the bottom end of each connecting rod is fixedly connected to the upper surface of the connecting frame, and a ball is fixedly connected to the upper surface of the connecting frame.

[0010] Optionally, the bottom surface of the mounting component is fixedly connected to a threaded component, the inner wall of the threaded component is threadedly connected to a sleeve, and the inner wall of the sleeve is fixedly connected to a retaining ring.

[0011] Optionally, a connecting sleeve is slidably connected to the inner wall of the fixing ring, and a limit ring is fixedly connected to the outer surface of the connecting sleeve.

[0012] Optionally, an ultrafiltration membrane is threadedly connected to the inner wall of the connecting sleeve, and a reaction vessel is threadedly connected to the outer surface of the ultrafiltration membrane.

[0013] In summary, this application includes the following beneficial technical effects:

[0014] 1. This utility model incorporates components such as a threaded cylinder, piston, inlet pipe, threaded plug, threaded rod, and infusion pipe. A cavity is created within the mounting component, with the piston placed on its inner wall. The inlet and infusion pipes facilitate the introduction of fluorescein into the cavity. The threaded plug is then rotated for sealing. The threaded rod connects to the threaded cylinder, and a guide rod limits the piston's movement, allowing it to descend under the influence of the threaded cylinder, extruding the fluorescein from the cavity and infusion pipe. This device, with its threaded cylinder, piston, and threaded rod, simplifies the entire operation by allowing fluorescein to be added for reaction, eliminating the need for prior injection of fluorescein, antibody injection, and membrane removal. This makes it convenient to use.

[0015] 2. This utility model, by setting up components such as a sliding block, spring, connecting rod, connecting frame, and ball, allows the ball to connect to the bottom end of the infusion tube under the action of the spring when the device is not discharging fluorescein into the infusion tube, sealing the opening at the bottom of the infusion tube. When fluorescein is input into the infusion tube, the inside of the infusion tube is compressed, causing the ball to open and the fluorescein to flow downwards until it reaches the pointed position on the bottom surface of the connecting frame. Through the pointed position, the fluorescein can fall quickly. After the fluorescein is discharged, the sliding block is reset under the action of the spring, which in turn drives the connecting rod to reset, and the connecting rod drives the connecting frame to reset. The connecting frame then drives the ball to block the bottom end of the infusion tube again. This allows the device to facilitate the opening and closing of the infusion tube through the movement of the piston above, making it easy for the fluorescein to drip into the reaction vessel below. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall three-dimensional structure in the embodiments of this application;

[0017] Figure 2 This is a schematic diagram of the structure of the main view in an embodiment of this application;

[0018] Figure 3 This is a schematic diagram of the connection relationship between the ultrafiltration membrane and the reaction vessel in an embodiment of this application;

[0019] Figure 4 This is a structural schematic diagram of the connection relationship between the threaded rod and the threaded cylinder in an embodiment of this application;

[0020] Figure 5 This is an embodiment of the present application. Figure 4 Enlarged structural diagram of section A in the middle.

[0021] Reference numerals: 1. Mounting component; 2. Cavity; 3. Threaded cylinder; 4. Piston; 5. Inlet pipe; 6. Threaded plug; 7. Threaded rod; 8. Infusion pipe; 9. Guide rod; 10. Sliding groove; 11. Sliding block; 12. Spring; 13. Connecting rod; 14. Connecting frame; 15. Ball; 16. Threaded component; 17. Sleeve; 18. Fixing ring; 19. Limiting ring; 20. Ultrafiltration membrane; 21. Reaction vessel; 22. Connecting sleeve. Detailed Implementation

[0022] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail.

[0023] This application discloses a fluorescently labeled antibody device. For example... Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5As shown, the device includes a mounting component 1, which has a cavity 2 inside. A piston 4 is slidably connected to the inner wall of the cavity 2, and a threaded cylinder 3 is fixedly connected to the inner wall of the piston 4. The cavity 2 is located inside the mounting component 1 to achieve the positioning effect of the cavity 2. The cavity 2 can hold the fluorescein. The piston 4 is placed on the inner wall of the cavity 2 and is set to a sliding connection. By moving the piston 4 up and down, the fluorescein inside the cavity 2 can be squeezed. The threaded cylinder 3 is installed on the inner wall of the piston 4 and is set to a fixed connection to achieve the positioning and installation effect of the threaded cylinder 3.

[0024] Please see Figure 3 The bottom surface of the mounting component 1 is fixedly connected to a threaded component 16, the inner wall of the threaded component 16 is threadedly connected to a sleeve 17, and the inner wall of the sleeve 17 is fixedly connected to a retaining ring 18. The threaded component 16 is installed on the bottom surface of the mounting component 1, and the sleeve 17 is installed on the inner wall of the threaded component 16, which is set as a threaded connection to realize the installation of the sleeve 17. The retaining ring 18 is set on the inner wall of the sleeve 17, which is set as a fixed connection to realize the positioning and installation effect of the retaining ring 18.

[0025] Please see Figure 3 A connecting sleeve 22 is slidably connected to the inner wall of the fixed ring 18. A limiting ring 19 is fixedly connected to the outer surface of the connecting sleeve 22. The connecting sleeve 22 is installed on the inner wall of the fixed ring 18, which is set as a sliding connection. The limiting ring 19 is installed on the surface of the connecting sleeve 22. When the connecting sleeve 22 is placed on the inner wall of the fixed ring 18, the limiting ring 19 contacts the fixed ring 18 to limit the connecting sleeve 22. The upper part is connected to the sleeve 17 through the threaded part 16, so that the bottom surface of the threaded part 16 contacts the upper surface of the connecting sleeve 22 to limit the connecting sleeve 22.

[0026] Please see Figure 3 The inner wall of the connecting sleeve 22 is threaded with an ultrafiltration membrane 20, and the outer surface of the ultrafiltration membrane 20 is threaded with a reaction dish 21. The ultrafiltration membrane 20 is installed below the connecting sleeve 22, and the connection between them is set as a thread. The ultrafiltration membrane 20 can be installed on the inner wall of the connecting sleeve 22 by rotating it. The reaction dish 21 is connected to the ultrafiltration membrane 20 to realize the installation of the reaction dish 21. The ultrafiltration membrane 20 is a polymer semipermeable membrane that can separate polymer colloids or suspended particles of a certain size from the solution. The reaction dish 21 is used to hold antibodies and fluorescein.

[0027] Please see Figure 5The bottom surface of the mounting part 1 has two sliding grooves 10, and the inner wall of each sliding groove 10 is slidably connected to a sliding block 11. The sliding groove 10 is opened on the bottom surface of the mounting part 1 to achieve the positioning effect of the sliding groove 10. The sliding block 11 is placed inside the sliding groove 10 and they are slidably connected. The contour of the sliding groove 10 can achieve the limiting effect of the sliding block 11.

[0028] Please see Figure 5 Each sliding block 11 has a spring 12 and a connecting rod 13 fixedly connected to its bottom surface. The bottom end of each spring 12 is fixedly connected to the inner bottom wall of the corresponding sliding groove 10. The outer surface of each connecting rod 13 is slidably connected to the inner wall of the corresponding sliding groove 10. By installing the spring 12 and the connecting rod 13 on the bottom surface of the corresponding sliding block 11, the spring 12 and the connecting rod 13 are positioned and installed. The movement of the sliding block 11 can compress the spring 12 and drive the connecting rod 13 to move. The spring 12 facilitates the reset of the sliding block 11. The bottom end of the spring 12 is connected to the inner bottom wall of the sliding groove 10 to limit the bottom end of the spring 12. The connecting rod 13 is connected to the mounting part 1 to limit the connecting rod 13.

[0029] Please see Figure 4 The upper surface of the mounting part 1 is threaded with a threaded plug 6. The inner bottom wall of the cavity 2 is fixedly connected with two guide rods 9. The outer surface of each guide rod 9 is slidably connected to the inner wall of the piston 4. The threaded plug 6 is installed on the upper surface of the mounting part 1 and set as a threaded connection. The bottom surface of the threaded plug 6 is provided with a sealing ring. When the threaded plug 6 is installed, it can seal the liquid inlet pipe 5. The guide rod 9 is installed on the inner bottom wall of the cavity 2 to achieve the positioning and installation effect of the guide rod 9. The guide rod 9 is connected to the piston 4. The guide rod 9 can ensure that the piston 4 will not rotate under the action of the threaded rod 7, and ensure that the piston 4 can only move up and down.

[0030] Please see Figure 5 A connecting frame 14 is provided below the mounting component 1. The bottom end of each connecting rod 13 is fixedly connected to the upper surface of the connecting frame 14. A ball 15 is fixedly connected to the upper surface of the connecting frame 14. The connecting frame 14 is installed below the mounting component 1, and the bottom ends of the two connecting rods 13 are connected to the upper surface of the connecting frame 14. By moving the connecting frame 14, the two connecting rods 13 can be moved, and the ball 15 is installed on the upper surface of the connecting frame 14. The surface of the ball 15 can be adapted to the infusion tube 8 to achieve a sealing effect on the infusion tube 8.

[0031] Please see Figure 4The inner wall of the threaded cylinder 3 is threaded with a threaded rod 7. The outer surface of the threaded rod 7 is rotatably connected to the inner wall of the mounting part 1. The inner wall of the mounting part 1 is fixedly installed with an inlet pipe 5 and an inlet pipe 8. The bottom end of the inlet pipe 5 and the top end of the inlet pipe 8 are both fixedly connected to the inner wall of the cavity 2. The threaded rod 7 is installed on the inner wall of the threaded cylinder 3 and is threadedly connected to it. The threaded rod 7 is also threadedly connected to the mounting part 1 to limit the movement of the threaded rod 7. The threaded cylinder 3 can be raised and lowered by rotating the threaded rod 7, thereby raising and lowering the piston 4. The inlet pipe 5 and the inlet pipe 8 are installed inside the mounting part 1 and are connected to the cavity 2 to facilitate the transfer of fluorescein.

[0032] The implementation principle of the fluorescently labeled antibody device in this application embodiment is as follows: Fluorescent material is pre-stored inside the cavity 2 via the inlet pipe 5, and a threaded plug 6 is installed to achieve sealing. First, the antibody is injected into the reaction dish 21. The reaction dish 21, ultrafiltration membrane 20, and connecting sleeve 22 are connected. The movable connecting sleeve 22 is placed inside the fixing ring 18, and the limiting ring 19 contacts the fixing ring 18 to limit the connection sleeve 22. The reaction dish 21 is placed inside the sleeve 17 via the threaded connection of the threaded component 16 to the sleeve 17. The threaded rod 7 is connected to the threaded cylinder 3 by rotation. The piston 4 is limited by the guide rod 9, allowing the piston 4 to move under the action of the threaded cylinder 3. As the pressure decreases, the sphere 15 is forced open, causing the connecting frame 14 to move. The connecting frame 14 then moves the connecting rod 13 and the sliding block 11, compressing the spring 12. At this time, the fluorescein in the cavity 2 and the infusion tube 8 is squeezed out, causing the fluorescein to drip onto the surface of the sphere 15. Through the pointed cone shape of the bottom surface of the connecting frame 14, the fluorescein automatically falls into the reaction dish 21. After the rotation of the threaded rod 7 stops, the spring 12 returns to its original position, moving the sphere 15 and sealing the infusion tube 8 again. After waiting for several minutes for the reaction to proceed, the solution is sent to a centrifuge for filtration. Under the action of the centrifuge, the unreacted fluorescent liquid is filtered out through the ultrafiltration membrane 20 and falls into the sleeve 17, while the other part remains inside the reaction dish 21.

[0033] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A fluorescently labeled antibody device, comprising a mounting component (1), characterized in that: The mounting component (1) has a cavity (2) inside. A piston (4) is slidably connected to the inner wall of the cavity (2). A threaded cylinder (3) is fixedly connected to the inner wall of the piston (4). A threaded rod (7) is threadedly connected to the inner wall of the threaded cylinder (3). The outer surface of the threaded rod (7) is rotatably connected to the inner wall of the mounting component (1). An inlet pipe (5) and an infusion pipe (8) are fixedly installed on the inner wall of the mounting component (1). The bottom end of the inlet pipe (5) and the top end of the infusion pipe (8) are both fixedly connected to the inner wall of the cavity (2). A threaded plug (6) is threadedly connected to the upper surface of the mounting component (1). Two guide rods (9) are fixedly connected to the inner bottom wall of the cavity (2). The outer surface of each guide rod (9) is slidably connected to the inner wall of the piston (4).

2. The fluorescently labeled antibody device according to claim 1, characterized in that: The bottom surface of the mounting component (1) has two sliding grooves (10), and a sliding block (11) is slidably connected to the inner wall of each sliding groove (10).

3. The fluorescently labeled antibody device according to claim 2, characterized in that: Each of the sliding blocks (11) has a spring (12) and a connecting rod (13) fixedly connected to its bottom surface. The bottom end of each spring (12) is fixedly connected to the inner bottom wall of the corresponding sliding groove (10), and the outer surface of each connecting rod (13) is slidably connected to the inner wall of the corresponding sliding groove (10).

4. The fluorescently labeled antibody device according to claim 3, characterized in that: A connecting frame (14) is provided below the mounting component (1), and the bottom end of each connecting rod (13) is fixedly connected to the upper surface of the connecting frame (14). A ball (15) is fixedly connected to the upper surface of the connecting frame (14).

5. The fluorescently labeled antibody device according to claim 1, characterized in that: The bottom surface of the mounting component (1) is fixedly connected to a threaded component (16), and the inner wall of the threaded component (16) is threadedly connected to a sleeve (17), and the inner wall of the sleeve (17) is fixedly connected to a retaining ring (18).

6. The fluorescently labeled antibody device according to claim 5, characterized in that: The inner wall of the fixed ring (18) is slidably connected to a connecting sleeve (22), and the outer surface of the connecting sleeve (22) is fixedly connected to a limit ring (19).

7. The fluorescently labeled antibody device according to claim 6, characterized in that: The inner wall of the connecting sleeve (22) is threaded with an ultrafiltration membrane (20), and the outer surface of the ultrafiltration membrane (20) is threaded with a reaction vessel (21).

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