Mouse stabilizing instrument for assisting imaging of fixed anesthetic mouse

Abstract

The utility model discloses a mouse constant-stability instrument for assisting in fixing and anesthetizing mouse imaging. The mouse constant-stability instrument comprises a limb pressing device, a constant-stability device and a bottom plate. The limb pressing device is provided with a Y-shaped fork and an ultra-thin transparent rubber band, and the rubber band can be fixed through a tightening piece to press the limbs of the mouse. A magnetic storage bin of the steady device is made of non-iron materials, a magnetic attraction block is arranged in the magnetic storage bin, and a magnetic storage groove is opened and closed through a sliding cover. The bottom plate is made of black iron, so that fluorescence imaging is facilitated. The device can be adsorbed on the bottom plate through the magnetic blocks to fix the mouse body position. The device is provided with three cylindrical magnetic storage grooves, and the number of magnets can be increased or decreased according to requirements. Except for the rubber band, the whole body is frosted black, and imaging interference is reduced. The four limbs can be fixed in the posture of exposing the abdomen or back of the mouse, operation is convenient, experimental results can be better obtained, and the mouse body position fixing device is suitable for fixing the mouse body position during auxiliary anaesthesia mouse imaging.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of animal experiment equipment, specifically relates to a mouse constant stabilizer for assisting fixed anesthetized mouse imaging. BACKGROUND

[0002] Animal experiment is an important link in medical teaching and research, and is generally used for toxicology, pharmacology, pathology, physiology and even psychological experimental research. Many experimental researches do not allow or need to be directly conducted on human bodies, and according to experimental requirements, similar human body research results can be produced on animals. Among various experimental animals, mice are favored by experimental personnel due to their body size, reproductive capacity and similar genes to humans. In the process of mouse live fluorescence imaging experiment, the mouse is generally anesthetized first, and then the limbs are fixed with adhesive tape to expose the complete abdomen or back, display tumor models or metastatic lymph node models, and then imaging is performed by using a biological imaging device (such as DPM, bioluminescence imaging instrument). However, the method of fixing the limbs of the mouse by using adhesive tape is simple, but it has the following technical defects which are not conducive to fluorescence imaging experiment: ① When preparing the adhesive tape block, the length of the adhesive tape block is often inconsistent, and the gloves of the experimenter are easily stuck, resulting in that it takes more time to fix the limbs of the mouse; ② After the adhesive tape is stuck, the adhesive tape may be popped off due to insufficient depth of anesthesia of the mouse, and the fixation is unreliable; ③ When the adhesive tape is torn off, the black bottom plate is easily damaged, which affects the fluorescence imaging effect. Based on the above problems, it is urgent to develop an auxiliary fixing instrument for fluorescence imaging experiment for fixing the limbs of anesthetized mice for fluorescence imaging. SUMMARY

[0003] To solve the above technical problems, the utility model provides a mouse constant stabilizer for assisting fixed anesthetized mouse imaging, which assists the imaging position of the mouse by fixing one limb of the mouse with a constant stabilizer, and the specific technical scheme is as follows:

[0004] A mouse constant stabilizer for assisting fixed anesthetized mouse imaging, the mouse constant stabilizer comprises a limb pressing device, a constant stabilizing device and a bottom plate;

[0005] The limb pressing device comprises a "Y" shaped fork and transparent pressing pieces installed at both ends of the "Y" shaped fork for pressing the limbs of the mouse; the bottom end of the "Y" shaped fork is provided with a connecting rod for connecting with the constant stabilizing device;

[0006] The constant stabilizing device comprises a magnetic storage bin and a magnetic attraction block installed in the magnetic storage bin, and the magnetic storage bin is made of non-ferrous material;

[0007] The bottom plate is a black iron bottom plate for facilitating fluorescence imaging, and the mouse constant stabilizer can be adsorbed on the bottom plate by the magnetic attraction block in the magnetic storage bin.

[0008] Further, the "Y" shaped fork includes a first branch and a second branch, and the transparent pressing member is wound on two prongs of the first branch to assist in body position fixation.

[0009] Further, a threaded hole is arranged on the second branch, and a bolt column extends through the threaded hole to contact the first branch to compress the transparent pressing member wound on the first branch.

[0010] Further, the transparent pressing member is an ultra-thin transparent rubber band, and the elasticity of the rubber band can match different circumferences of mouse limbs.

[0011] Further, the magnetic storage bin includes a shell, a magnetic storage groove and a sliding cover, and the magnetic storage groove is a cavity structure arranged inside the magnetic storage bin.

[0012] Further, the shell is a block-shaped body with arc-shaped surfaces on two sides, the arc-shaped surfaces are concave arc surfaces for easy grabbing, and the remaining four surfaces are regular flat surfaces.

[0013] Further, at least one magnetic storage groove is arranged inside the magnetic storage bin.

[0014] Further, the sliding cover is arranged directly above the magnetic storage groove to open and close the storage groove, and the sliding cover is provided with a sliding groove on the left and right sides along the sliding direction.

[0015] Further, the shell is provided with a clamping groove matched with the sliding groove for limiting the sliding cover.

[0016] Further, the outer surface of the constant stabilizer is entirely black and frosted to reduce interference with imaging.

[0017] The working principle of the utility model is as follows: a mouse constant stabilizer for assisting in fixing anesthetized mice for imaging, the device comprises a limb pressing device (1), a constant stabilizer (2) and a bottom plate (3); the limb pressing device (1) comprises a "Y" shaped fork (12) and a transparent pressing member mounted at two ends of the "Y" shaped fork for pressing mouse limbs; the bottom end of the "Y" shaped fork (12) is provided with a connecting rod for connecting with the constant stabilizer (2); the constant stabilizer comprises a magnetic storage bin and a magnetic attraction block mounted in the magnetic storage bin, and the magnetic storage bin is made of non-ferrous material; the bottom plate (3) is a black iron bottom plate (3) for facilitating fluorescence imaging, and the device can be adsorbed on the bottom plate (3) through the magnetic attraction block in the magnetic storage bin.

[0018] When the device is used to assist in fixing the body position of mice, the specific operation is as follows:

[0019] (1) Device preparation, first wrap the ultra-thin transparent rubber band (11) at both ends of the first branch (121) respectively, then pass through the threaded hole provided on the second branch (122) through the tightening member (5) to extend to contact and compress the thin transparent rubber band (11), fix and install the thin transparent rubber band (11) on the first branch (121), then open the sliding cover (23) on the shell (21), put the magnetic block (4) into the magnetic storage groove (22) in the magnetic storage bin (according to the actual need to adjust the magnetic force to store different number of magnets), and finally close the sliding cover (23) to seal the magnetic storage bin to complete the assembly.

[0020] (2) Place the anesthetized mouse on the black base plate (3).

[0021] (3) Fix the mouse: fix the mouse limbs in a posture to expose the complete abdomen or back.

[0022] The specific operation of fixing the mouse limbs, the mouse is placed on the black base plate (3) in the posture shown in Figure 3 or Figure 4 , and the upper limbs and / or lower limbs of the mouse to be fixed are stretched out, and the ultra-thin transparent rubber band (11) on the constant stabilizer Y-shaped fork (12) is pressed on the upper limbs and / or lower limbs of the mouse, and the constant stabilizer is fixed and adsorbed on the base plate (3) by the magnetic block (4) to realize the fixation of the mouse body position.

[0023] Compared with the prior art, the beneficial effects of the utility model are:

[0024] (1) Fixing the anesthetized mouse limbs has the advantages of being fast, flexible, reliable, recyclable, and reducing mouse foot injury;

[0025] (2) When the constant stabilizer is used to fix the mouse limbs, except that the transparent tape directly contacts the fixed part, the rest does not directly contact the mouse, which can effectively reduce imaging interference and promote standardized operation, which is conducive to the stability of imaging results;

[0026] (3) Small, easy to match different use scenarios, promote standardized operation;

[0027] (4) Low production cost, easy to promote on a large scale. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a structure schematic view of the utility model;

[0029] Figure 2 It is a component split view of the utility model;

[0030] Figure 3 It is a use state diagram example one of the utility model;

[0031] Figure 4 It is the use state diagram example two of the utility model;

[0032] Figure 5 It is the use effect drawing of the utility model;

[0033] In the drawing, 1-limb compression device; 11-elastic band; 12-Y type fork; 121-first branch; 122-second branch; 2-constant stabilizer; 21-housing; 211-side; 22-magnetic storage groove; 23-sliding cover; 3-bottom plate; 4-magnetic block; 5-tightening piece; 6-connecting rod. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical scheme and advantage of the utility model more clear and apparent, the utility model is further explained in detail below in combination with specific implementation manners and with reference to the drawings. It should be understood that these descriptions are only exemplary, and are not intended to limit the scope of the utility model. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the utility model.

[0035] Embodiment 1: a mouse constant stabilizer for assisting fixation of anesthetized mouse imaging, the mouse constant stabilizer comprising a limb compression device 1, a constant stabilizer 2 and a bottom plate 3.

[0036] The limb compression device 1 is connected with the horizontal stabilizer through a connecting rod 6, and comprises a Y type fork 12 and a transparent compression piece; the Y type fork 12 comprises a first branch 121 and a second branch 122, the first branch 121 comprises two branches for mounting the transparent compression piece, the two ends of the transparent compression piece are wound on the first branch 121 for limiting the upper limbs and / or lower limbs of the mouse, and the lower surface of the first branch 121 is at the same horizontal height as the lower surface of the constant stabilizer 2; the second branch 122 is used for fixing and locking the transparent compression piece to avoid the transparent compression piece from falling off due to external force; a through screw hole (provided with an internal thread) is arranged on the second branch 122, and a tightening piece 5 provided with an external thread (adapted to the screw hole) penetrates the screw hole, and the tip of the tightening piece 5 is in contact with the transparent compression piece wound on the first branch 121 for compressing the transparent compression piece (such as Figure 1 ), and the length of the screw column of the tightening piece 5 is greater than the straight line length between the first branch 121 and the second branch 122. The transparent compression piece is an ultrathin transparent elastic band 11, which can match the limbs of mice with different circumferences through the elasticity of the elastic band 11.

[0037] The constant stabilizer 2 comprises a magnetic storage bin and a magnetic suction block mounted in the magnetic storage bin; the magnetic storage bin comprises a housing 21, a magnetic storage groove 22 and a sliding cover 23, wherein the magnetic storage groove 22 is a cavity structure arranged inside the magnetic storage bin, and the magnetic storage groove 22 is like Figure 2The cylindrical magnetic storage groove 22 shown, the magnetic storage groove 22 includes three, each groove diameter 1cm, high 2cm, the number of magnets placed in the magnetic storage groove 22 can be increased or decreased according to the actual demand for the device to be magnetically attracted to the bottom plate 3; the shell 21 is a square block made of non-ferrous material, the two side surfaces 211 of the square block are arc surfaces, the arc surfaces are concave arc surfaces for easy grabbing, and the remaining four surfaces are regular flat surfaces. The sliding cover 23 is arranged on the upper surface of the square block and is located directly above the magnetic storage groove 22 for closing the magnetic storage groove 22, and the sliding cover 23 is like Figure 2 As shown, the left and right sides of the sliding direction are provided with sliding grooves, and the upper surface of the shell 21 is provided with clamping grooves matched with the sliding grooves for limiting the sliding cover 23.

[0038] The bottom plate 3 is a black iron bottom plate 3 for facilitating fluorescence imaging, and the mouse constant stabilizer can be attracted to the bottom plate 3 by the magnetic attraction block in the magnetic storage bin.

[0039] The magnetic block 4 is a cylindrical magnetic block 4 matched with the magnetic storage groove 22, and the size of the magnetic block 4 is smaller than that of the magnetic storage groove 22.

[0040] After the sliding cover 23 of the device is covered, the entire device is a matte black surface except for the transparent rubber band 11 part, which reduces interference with imaging and can better obtain experimental results.

[0041] Embodiment 2: Another embodiment of a mouse constant stabilizer for assisting in fixing anesthetized mouse imaging, the limb pressing device 1 of the device is only provided with a first branch 121, the lower surface of the first branch 121 is at the same horizontal height as the lower surface of the constant stabilizer 2, the ultra-thin transparent rubber band 11 is a ring-shaped rubber band 11, which is directly sleeved on the two forks of the first branch 121 during use to complete installation, and the elastic length of the sleeved ring-shaped rubber band 11 is greater than the distance between the two forks of the first branch 121.

[0042] Embodiment 3: Another embodiment of a mouse constant stabilizer for assisting in fixing anesthetized mouse imaging, the magnetic storage bin of the constant stabilizer 2 of the device is only provided with one magnetic storage groove 22, and the magnetic block 4 is a round cake, which is stacked into the magnetic storage groove 22 to increase the magnetic attraction for magnetically attracting the device to the bottom plate 3.

[0043] It should be understood that the above specific embodiments of the present application are only used for illustrative or explanatory purposes of the principles of the present application, and do not constitute a limitation on the present application. Therefore, any modification, equivalent replacement, improvement, etc. made without departing from the spirit and scope of the present application shall be included in the protection scope of the present application. In addition, the appended claims of the present application are intended to cover all changes and modifications falling within the scope and boundaries of the appended claims, or the equivalent forms of such scope and boundaries.

Claims

1. A mouse stabilization device for assisting in imaging of anesthetized mice, characterized in that, The mouse constant stabilizer comprises a limb pressing device (1), a constant stabilizer (2) and a bottom plate (3); The limb pressing device (1) comprises a "Y" type fork (12) and transparent pressing members mounted at both ends of the "Y" type fork for pressing the limbs of the mouse; the bottom end of the "Y" type fork (12) is provided with a connecting rod (6) for connecting with the constant stabilizer (2); The constant stabilizer (2) comprises a magnetic storage bin and a magnetic suction block mounted in the magnetic storage bin, and the magnetic storage bin is made of non-ferrous material; The bottom plate (3) is a black iron bottom plate (3) for facilitating fluorescence imaging, and the mouse constant stabilizer can be adsorbed on the bottom plate (3) by the magnetic suction block in the magnetic storage bin.

2. The mouse homeothermic calorimeter for imaging anesthetized mice according to claim 1, wherein, The "Y" type fork (12) comprises a first branch (121) and a second branch (122), and the transparent pressing members are wound on the two branches of the first branch (121) for assisting body position fixation.

3. The mouse homeothermic calorimeter for imaging anesthetized mice according to claim 2, wherein, The second branch (122) is provided with a threaded hole, and a tightening member (5) penetrates through the threaded hole and extends to contact the first branch (121) for pressing the transparent pressing members wound on the first branch (121).

4. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 1, wherein, The transparent pressing member is an ultra-thin transparent rubber band (11), which can match the limbs of mice with different circumferences by elasticity.

5. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 1, wherein, The magnetic storage bin comprises a shell (21), a magnetic storage groove (22) and a sliding cover (23), and the magnetic storage groove (22) is a cavity structure arranged inside the magnetic storage bin.

6. The mouse homeothermic calorimeter for imaging anesthetized mice according to claim 5, wherein, The shell (21) is a block-shaped body with arc-shaped sides (211), the arc-shaped sides are concave arc-shaped sides for easy grabbing, and the other four sides are regular flat surfaces.

7. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 5, wherein, At least one magnetic storage groove (22) is arranged inside the magnetic storage bin.

8. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 5, wherein, The sliding cover (23) is arranged above the magnetic storage groove (22) for opening and closing the storage groove, and the sliding cover (23) is provided with sliding grooves on the left and right sides along the sliding direction.

9. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 5, wherein, The shell (21) is provided with clamping grooves matched with the sliding grooves for limiting the sliding cover (23).

10. The mouse homeothermic calorimeter for imaging anesthetized mice of claim 1, wherein, The outer surface of the constant stabilizer is matte black, which can reduce the interference with imaging.

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