Nanometer biological material transfection reagent preparation device
By introducing a stirring component and an ultrasonic crushing device into the nanobiomaterial transfection reagent preparation device, the problems of long mixing time and inhomogeneity were solved, achieving efficient mixing and particle crushing, and improving the preparation quality.
Patent Information
- Application Number
- CN202423120712.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-18
- Publication Date
- 2025-11-18
- Estimated Expiration
- 2034-12-18
AI Technical Summary
In the preparation of existing nanobiomaterial transfection reagents, the long mixing time and poor fluidity lead to uneven mixing and affect the preparation quality.
The design incorporates a stirring assembly, including stirring blades and an open structure, to generate and break bubbles. Combined with an ultrasonic crushing device, this improves fluidity and mixing uniformity. The tumbling assembly further enhances mixing efficiency through reciprocating motion.
It significantly improves the mixing efficiency and preparation quality of nanobiomaterial transfection reagents, ensuring solution homogeneity and particle breakage effect.
Smart Images
Figure CN223555918U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to nanometer biological material transfection reagent preparation technical field, especially, relate to a nanometer biological material transfection reagent preparation device. BACKGROUND
[0002] Nanometer biological material transfection reagent preparation device is a kind of equipment specially used for preparing nanometer biological material transfection reagent, and the device usually combines advanced biotechnology and nanotechnology to ensure the efficiency and safety of transfection reagent.
[0003] The existing nanometer biological material transfection reagent needs to mix biological material (such as DNA, RNA or protein) with carrier material (such as lipid, polymer, etc.) when preparing, but the traditional mixing is only mixed by the way of stirring rod, which makes the fluidity of liquid poor, so a lot of mixing time is needed, and the insufficient fluidity of solution will affect the uniformity of mixing, reduce the preparation quality of nanometer biological material transfection reagent, therefore we propose a nanometer biological material transfection reagent preparation device. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a nanometer biological material transfection reagent preparation device, by setting stirring assembly, specifically, solution is stirred when stirring blade rotates, because the contact area of stirring blade and solution is larger, so the flow effect of solution can be improved, and the opening set on stirring blade can produce the effect of bubble during stirring, break after producing bubble, can improve mixing effect, at the same time, internal biological particles are broken, make solution mixing more uniform to improve preparation quality, solve the problem that the existing traditional mixing is only mixed by the way of stirring rod, which makes the fluidity of liquid poor, so a lot of mixing time is needed, and the insufficient fluidity of solution will affect the uniformity of mixing, reduce the preparation quality of nanometer biological material transfection reagent.
[0005] To solve the above technical problems, the utility model is realized by the following technical schemes:
[0006] The utility model is a kind of nanometer biological material transfection reagent preparation device, including preparation box, the preparation box top is fixedly connected with two feed pipes, the preparation box top center is fixedly connected with motor, the preparation box is internally provided with stirring assembly, and the stirring assembly includes the shaft that is arranged in the center of preparation box interior, and the shaft outer surface is fixedly connected with fixed frame;
[0007] The shaft top is fixedly connected with the motor bottom output end by coupling, the fixed frame bottom is fixedly connected with three vertical rods, three vertical rods outer surfaces are fixedly connected with stirring blades, the stirring blade is internally provided with opening, and the opening is spirally arranged.
[0008] Further, the preparation tank bottom is fixedly connected with a discharge pipe, a valve is arranged outside the discharge pipe, and an ultrasonic crushing device is arranged behind the discharge pipe, one end of the top of the ultrasonic crushing device penetrates through the preparation tank bottom and extends to the inside.
[0009] Further, the rotating shaft bottom is fixedly connected with a conical block, the eccentric bottom of the conical block is fixedly connected with a convex shaft, the outer side of the conical block is provided with a conical surface, and a turning assembly is arranged below the conical block.
[0010] Further, the turning assembly comprises a reciprocating rod, two turning discs are fixedly connected to the outer surface of the reciprocating rod, an inclined ring is fixedly connected to the top of the reciprocating rod, a plurality of leakage grooves are arranged in the bottom of the inclined ring, and a limiting ring is fixedly connected to the bottom of the outer surface of the reciprocating rod.
[0011] Further, a sleeve is arranged outside the limiting ring, a spring is fixedly connected to the bottom of the inner wall of the sleeve, the top of the spring is fixedly connected with the bottom of the limiting ring, a plurality of supporting frames are fixedly connected to the bottom of the sleeve, and the bottom of the supporting frame is fixedly connected with the inner wall bottom of the preparation tank.
[0012] Further, the bottom of the convex shaft is in contact with the top of the inclined ring, the outer side of the turning disc is provided with a conical surface, and there is a large gap between the inner wall of the bottom center of the sleeve and the surface of the reciprocating rod.
[0013] The utility model has the following beneficial effects:
[0014] 1. The utility model discloses a stirring assembly, which can stir the solution when the stirring blade rotates. Because the stirring blade has a large contact area with the solution, the flow effect of the solution can be improved. The holes in the stirring blade can generate bubbles during stirring, which can improve the mixing effect and break the biological particles inside, making the solution mixing more uniform and improving the preparation quality.
[0015] 2. The utility model discloses a turning assembly, which can rotate the convex shaft together with the conical block during the rotation of the rotating shaft. At this time, the reciprocating rod drives the turning disc to move up and down reciprocally, so that the turning disc stirs the solution during movement. The liquid at the bottom can also be stirred better, further improving the mixing efficiency.
[0016] Of course, any product implementing the utility model does not necessarily need to achieve all the advantages mentioned above. DRAWINGS
[0017] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed for the description of the embodiments will be briefly introduced as follows. Obviously, the drawings described below are only some of the embodiments of the present application, and not all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application.
[0018] Figure 1 The present application is a whole structure schematic diagram.
[0019] Figure 2 The present application is a preparation box front view structure schematic diagram.
[0020] Figure 3 The present application is Figure 2 The present application is an enlarged structure schematic diagram of A.
[0021] Figure 4 The present application is Figure 2 The present application is an enlarged structure schematic diagram of B.
[0022] Figure 5 The present application is a top structure schematic diagram of the turning assembly.
[0023] In the drawings, the component list represented by each reference numeral is as follows:
[0024] 1, preparation box; 11, feed pipe; 12, stirring assembly; 121, rotating shaft; 211, conical block; 212, convex shaft; 122, fixing frame; 123, vertical rod; 124, stirring blade; 125, opening; 13, turning assembly; 131, reciprocating rod; 132, turning disc; 133, inclined ring; 331, leakage groove; 134, limiting ring; 135, sleeve; 351, spring; 352, support frame; 14, ultrasonic crushing device; 2, motor; 3, discharge pipe; 31, valve. DETAILED DESCRIPTION
[0025] The technical scheme in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, and not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application.
[0026] Please refer to Figures 1-5As shown, the utility model is a kind of nanometer biomaterial transfection reagent preparation device, including preparation box 1, two feed pipes 11 are fixedly connected on the top of preparation box 1, motor 2 is fixedly connected at the top center of preparation box 1, stirring assembly 12 is arranged in preparation box 1, and stirring assembly 12 includes the rotating shaft 121 being arranged at the center of preparation box 1, and fixed frame 122 is fixedly connected on the outer surface of rotating shaft 121;
[0027] Rotating shaft 121 top is fixedly connected with motor 2 bottom output end by coupling, and three vertical rods 123 are fixedly connected on the bottom of fixed frame 122, three vertical rods 123 outer surface is fixedly connected with stirring blade 124, and opening 125 is formed in stirring blade 124, and opening 125 is spirally arranged, by setting stirring assembly 12, specifically, when stirring blade 124 rotates, solution is agitated, because the contact area of stirring blade 124 and solution is larger, therefore the flow effect of solution can be improved, and the opening 125 arranged on stirring blade 124 can produce the effect of bubble during stirring, and after bubble is produced, breakage is produced, can improve mixing effect, simultaneously, internal biological particles are broken, make solution mixing more uniform and improve the quality of preparation.
[0028] Preparation box 1 bottom is fixedly connected with discharge pipe 3, valve 31 is arranged on the outside of discharge pipe 3, ultrasonic crushing equipment 14 is arranged behind discharge pipe 3, and one end of ultrasonic crushing equipment 14 top is penetrated through the bottom of preparation box 1 and extends to the inside, and when ultrasonic crushing equipment 14 is used, the mechanical vibration of ultrasonic wave can produce shear force in material inside, and the shear force can overcome the interaction between the atoms or molecules of material, to cause material to break or crush, and the mixture is dispersed into nanometer level particles by ultrasonic crushing mode, to reach good preparation quality.
[0029] Rotating shaft 121 bottom is fixedly connected with conical block 211, and convex shaft 212 is fixedly connected at eccentric position on the bottom of conical block 211, and the outer side of conical block 211 is conical surface, and turnover assembly 13 is arranged below conical block 211, and convex shaft 212 is used to push inclined ring 133, to make inclined ring 133 can smoothly drive reciprocating rod 131 to move downward.
[0030] Turnover assembly 13 includes reciprocating rod 131, two turnover discs 132 are fixedly connected on the outer surface of reciprocating rod 131, inclined ring 133 is fixedly connected on the top of reciprocating rod 131, a plurality of leakage grooves 331 are formed in the bottom of inclined ring 133, and limiting ring 134 is fixedly connected on the bottom of the outer surface of reciprocating rod 131, by setting turnover assembly 13, specifically, when rotating shaft 121 rotates, conical block 211 drives convex shaft 212 to rotate, at this moment, reciprocating rod 131 drives turnover disc 132 to reciprocate up and down, to make turnover disc 132 agitate solution when moving, and the liquid at the bottom can also be agitated better, to improve mixing efficiency again.
[0031] The outer side of the limiting ring 134 is provided with a sleeve 135, the inner wall bottom of the sleeve 135 is fixedly connected with a spring 351, the top of the spring 351 is fixedly connected with the bottom of the limiting ring 134, the bottom of the sleeve 135 is fixedly connected with a plurality of support frames 352, the bottom of the support frame 352 is fixedly connected with the inner wall bottom of the preparation box 1, and the sleeve 135 can limit the limiting ring 134, so that the limiting ring 134 and the reciprocating rod 131 move in a vertical manner.
[0032] The bottom of the convex shaft 212 is in contact with the top of the inclined ring 133, the outer side of the turnover disc 132 is provided with a conical surface, and there is a large gap between the inner wall of the bottom center of the sleeve 135 and the surface of the reciprocating rod 131. The conical surface provided on the outer side of the turnover disc 132 can better stir the solution, and can also reduce the residue of the solution.
[0033] One specific application of the embodiment is:
[0034] In use, the biological material and the carrier material are respectively placed into the preparation box 1 from the two feed pipes 11, and the motor 2 is started to drive the rotating shaft 121 to rotate, so that the rotating shaft 121 drives the stirring blade 124 to rotate through the vertical rod 123. At this time, the stirring blade 124 stirs the solution. Since the stirring blade 124 has a large contact area with the solution, the flow effect of the solution can be improved. The holes 125 provided on the stirring blade 124 can generate bubbles during stirring. After the bubbles are generated, they are broken, which can improve the mixing effect and break the internal biological particles. The ultrasonic crushing device 14 is started during the stirring process. When the ultrasonic crushing device 14 is used, the mechanical vibration of the ultrasonic wave generates a shearing force inside the material. This shearing force can overcome the interaction between the atoms or molecules of the material, thereby causing the material to break or be crushed. The mixture is dispersed into nanoparticles by ultrasonic crushing, thereby achieving good preparation quality. During the rotation of the rotating shaft 121, the conical block 211 drives the convex shaft 212 to rotate together. The convex shaft 212 slides on the top of the inclined ring 133. When the convex shaft 212 moves to the highest position of the inclined ring 133, it pushes the inclined ring 133 downward. At this time, the reciprocating rod 131 drives the limiting ring 134 to move downward in the sleeve 135. The limiting ring 134 extrudes the spring 351. When the convex shaft 212 moves away from the high position of the inclined ring 133, the spring 351 pushes the reciprocating rod 131 upward by the elastic effect, and the reciprocating rod 131 drives the turnover disc 132 to move up and down, so that the turnover disc 132 stirs the solution during movement. The liquid at the bottom can also be better stirred, thereby improving the mixing efficiency. The leakage groove 331 in the inclined ring 133 is used to assist in discharging the solution, so that the solution does not remain in the inclined ring 133. The bottom of the sleeve 135 is provided with a hollow structure, which can also make the solution flow out smoothly. When it is necessary to discharge the solution, the valve 31 on the discharge pipe 3 is opened, so that the solution can be discharged.
[0035] In the description of the present specification, the description referring to the terms "one embodiment", "an example", "a specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0036] The preferred embodiments of the present application disclosed above are only used to help explain the present application. The preferred embodiments do not describe all the details and limit the present application to the specific embodiments described. Obviously, according to the content of the present specification, many modifications and changes can be made. The present specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present application, so that the persons skilled in the art can well understand and utilize the present application. The present application is limited by the claims and the entire scope and equivalents thereof.
Claims
1. A device for preparing nanobiomaterial transfection reagents, comprising a preparation chamber (1), wherein two feed pipes (11) are fixedly connected to the top of the preparation chamber (1), and a motor (2) is fixedly connected to the center of the top of the preparation chamber (1), characterized in that: The preparation box (1) is equipped with a stirring assembly (12), which includes a rotating shaft (121) located at the center of the preparation box (1), and a fixing frame (122) is fixedly connected to the outer surface of the rotating shaft (121). The top of the rotating shaft (121) is fixedly connected to the bottom output end of the motor (2) via a coupling. Three vertical rods (123) are fixedly connected to the bottom of the fixed frame (122). Stirring blades (124) are fixedly connected to the outer surfaces of the three vertical rods (123). An opening (125) is provided inside the stirring blade (124), and the opening (125) is spirally arranged.
2. The apparatus for preparing nanobiomaterial transfection reagents according to claim 1, characterized in that, The bottom of the preparation box (1) is fixedly connected to a discharge pipe (3), a valve (31) is provided on the outside of the discharge pipe (3), and an ultrasonic crushing device (14) is provided behind the discharge pipe (3). One end of the top of the ultrasonic crushing device (14) penetrates the bottom of the preparation box (1) and extends into the interior.
3. The apparatus for preparing nanobiomaterial transfection reagents according to claim 2, characterized in that, A conical block (211) is fixedly connected to the bottom of the rotating shaft (121), and a convex shaft (212) is fixedly connected to the eccentric part of the bottom of the conical block (211). The outer side of the conical block (211) is set with a conical surface, and a flipping component (13) is set below the conical block (211).
4. The apparatus for preparing nanobiomaterial transfection reagents according to claim 3, characterized in that, The flipping assembly (13) includes a reciprocating rod (131), two flipping discs (132) are fixedly connected to the outer surface of the reciprocating rod (131), an inclined ring (133) is fixedly connected to the top of the reciprocating rod (131), a plurality of slots (331) are opened at the bottom of the inclined ring (133), and a limit ring (134) is fixedly connected to the bottom of the outer surface of the reciprocating rod (131).
5. The apparatus for preparing nanobiomaterial transfection reagents according to claim 4, characterized in that, A sleeve (135) is provided on the outside of the limiting ring (134), and a spring (351) is fixedly connected to the bottom of the inner wall of the sleeve (135). The top of the spring (351) is fixedly connected to the bottom of the limiting ring (134).
6. The apparatus for preparing nanobiomaterial transfection reagents according to claim 5, characterized in that, The bottom of the sleeve (135) is fixedly connected to several support frames (352), and the bottom of the support frames (352) is fixedly connected to the bottom of the inner wall of the preparation box (1).
7. The apparatus for preparing nanobiomaterial transfection reagents according to claim 5, characterized in that, The bottom of the convex shaft (212) contacts the top of the inclined ring (133), the outer side of the flipping disk (132) is set with a conical surface, and there is a large gap between the inner wall of the bottom center of the sleeve (135) and the surface of the reciprocating rod (131).