Purification device for detection based on QuEChERS purification technology

Abstract

The utility model relates to the technical field of residue detection, and discloses a QuEChERS purification technology-based purification device for detection, which comprises a working table, a linear guide rail I is fixedly mounted at the top of the working table, a mounting plate is arranged right above the linear guide rail I in a matching manner, a mixing and separating mechanism is connected to the linear guide rail I in a matching and sliding manner, and the mixing and separating mechanism is connected to the working table. And an uncovering assembly, a liquid injection assembly and an adding assembly are sequentially connected to the mounting plate from left to right, a mechanical arm is fixedly mounted at the center of the bottom of the connecting plate in a matched mode, and the storage rack is slidably connected to the first linear guide rail in a matched mode. The rapid extraction, mixing and centrifugal separation of a sample and the adding process of a purifying agent can be efficiently completed, impurities and interfering substances in the sample are effectively removed, so that the detection sensitivity and selectivity are improved, and the method is suitable for detecting pollutants such as plasticizers, pesticide residues and animal residues in various samples such as food and drugs, agricultural products and environmental samples and has a wide application prospect. And the requirement of rapid detection can be met.

Description

TECHNICAL FIELD

[0001] The utility model relates to residual detection technical field, concretely is a kind of purification device based on QuEChERS purification technique for detection. BACKGROUND

[0002] QuEChERS purification technique is a kind of fast, efficient, low-cost sample pretreatment method, is widely used in food and environmental sample residue detection.The core of QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique is its fast, simple operation process and good purification effect, it removes moisture and lipids in sample by using adsorbent and dispersant, to achieve the purpose of purification.

[0003] However, traditional manual operation not only time-consuming and labor-consuming, and easy to introduce operation error, influence the accuracy of detection result, since QuEChERS method needs the cooperation of multiple reagents and equipment, this increases the equipment investment and operation complexity of laboratory to some extent, QuEChERS method involves steps including sample extraction, purification and concentration and multiple links, each link needs specific reagent and equipment, this not only increases the economic burden of laboratory, also puts forward higher requirement to the technical level of operator.

[0004] Based on this, the utility model designs a kind of purification device based on QuEChERS purification technique for detection to solve the above problems. UTILITY MODEL CONTENT

[0005] The utility model is to provide a kind of purification device based on QuEChERS purification technique for detection to solve the problems presented in the above background technique.

[0006] In order to solve the above technical problems, the utility model provides technical scheme as follows: A kind of purification device for detection based on QuEChERS purification technique, including workbench, the top of the workbench is fixedly installed with linear guide rail one, linear guide rail one is matched to be provided with mounting plate directly above, the bottom of the mounting plate is fixedly connected with support lug plate at left and right two ends, the mounting plate is fixedly connected on workbench by support lug plate, linear guide rail one is matched to be slidably connected with mixed separation mechanism, the mounting plate is sequentially connected with uncovering subassembly, liquid injection subassembly and adding subassembly from left to right, the mounting plate is provided with empty slot on the right side of adding subassembly, straight line guide rail two is fixedly installed at the edge of empty slot front and back two sides, connecting plate is matched to be slidably connected on linear guide rail two, the center position of the bottom of connecting plate is matched fixedly installed with manipulator, the output end of the bottom of manipulator is matched to be connected with extraction tube, the right side of mixed separation mechanism is provided with storage rack, the storage rack is matched to be slidably connected on linear guide rail one, test tube is inserted in the storage rack;

[0007] The mixed separation mechanism includes a bottom plate, a centrifugal separation assembly, and an extraction mixing assembly. The bottom plate is slidably connected to the linear guide rail one. The center position of the top of the bottom plate is slidably connected with the centrifugal separation assembly. The top of the centrifugal separation assembly is matched to be connected with the extraction mixing assembly.

[0008] Preferably, the mixed separation mechanism moves through the linear guide rail one and corresponds to the uncovering subassembly, the liquid injection subassembly and the adding subassembly. The manipulator corresponds to the mixed separation mechanism and the storage rack through the linear guide rail two.

[0009] Preferably, the centrifugal separation assembly includes a support top plate, a centrifugal motor and a limiting block. The bottom of the support top plate is matched to be connected with the centrifugal motor. The bottom of the centrifugal motor is fixedly installed on the top of the bottom plate. The support top plate bottom is provided with a plurality of limiting blocks around the centrifugal motor.

[0010] Preferably, the top of the bottom plate is provided with a limiting circular groove corresponding to the limiting block. The limiting block is slidably connected in the limiting circular groove. The top of the support top plate is fixedly connected with a plurality of limiting seats at equal intervals.

[0011] Preferably, the extraction mixing assembly includes a mixing cylinder, a piston rod, a storage plate and a limiting plate. The bottom of the mixing cylinder is fixedly connected at the center position of the top of the support top plate. The top of the mixing cylinder is movably connected with the piston rod. The top end of the piston rod is fixedly connected with the storage plate for placing the sampling tube. The outer wall of the piston rod is matched to be fixedly connected with the limiting plate.

[0012] Preferably, the cover opening assembly comprises a push cylinder, a cover opening seat and cover opening grooves, the push cylinder is fixedly installed on the top of the mounting plate, the output end of the push cylinder penetrates through the mounting plate and is fixedly connected with the cover opening seat, and the bottom of the cover opening seat is provided with a plurality of cover opening grooves.

[0013] Preferably, the liquid injection assembly comprises a plurality of liquid injection pipes, and the plurality of liquid injection pipes correspond to the sample tubes placed on the placing plate.

[0014] Preferably, the adding assembly comprises a plurality of adding pipes, and the plurality of adding pipes correspond to the sample tubes placed on the placing plate.

[0015] Compared with the prior art, the utility model has the beneficial effects that:

[0016] Firstly, the utility model cooperates the mixing and separating mechanism, the cover opening assembly, the liquid injection assembly, the adding assembly and the manipulator, after the sample is placed in the sample tube, the sample is limited on the extraction and mixing assembly and is moved to the cover opening assembly to open the cover by the mixing and separating mechanism, then the sample tube is moved to below the liquid injection assembly, the reagent is injected, and then the sample tube is moved to below the adding assembly to add the extractant, the extraction and mixing assembly is started, after the sample is mixed with the extractant, the centrifugal motor works to centrifugalize, after centrifugalization, the manipulator sucks the supernatant to detect and analyze, the sample pretreatment is efficiently and accurately completed, the impurities and interfering substances in the sample can be effectively removed, the reliability of the detection result is ensured, the manual intervention is reduced, the detection efficiency and accuracy are improved, the advanced QuEChERS technology is integrated, the speed of sample treatment is improved, and the interfering substances can be effectively removed in the purification process, and the accuracy of detection data is ensured.

[0017] Secondly, the utility model realizes the automatic operation of the device by using the linear guide rail and the manipulator, reduces the labor intensity of the operator, improves the operation accuracy and repeatability, in addition, the modular design of the device makes the maintenance and upgrading more convenient, the corresponding assembly can be replaced according to different detection requirements, the flexibility and application range of the device are increased, the sample treatment time is greatly shortened while the detection quality is ensured, and the rapid detection requirement is met. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 It is a whole structure schematic view of the utility model;

[0019] Figure 2 It is a front view structure schematic view of the utility model;

[0020] Figure 3 It is a structure schematic view of the mixing and separating mechanism in the utility model;

[0021] Figure 4 It is a structure schematic view of the utility modelFigure 2 Enlarged structural schematic view at A in the figure;

[0022] Figure 5 Structure schematic view of the uncapping assembly in the utility model;

[0023] Figure 6 Structure schematic view of the liquid injection assembly in the utility model;

[0024] Figure 7 Structure schematic view of the adding assembly in the utility model.

[0025] Wherein: 1, workbench; 2, linear guide rail one; 3, mounting plate; 4, support ear plate; 5, mixed separation mechanism; 501, bottom plate; 502, centrifugal separation assembly; 5021, support top plate; 5022, centrifugal motor; 5023, limit block; 503, extraction mixing assembly; 5031, mixed cylinder; 5032, piston rod; 5033, storage plate; 5034, limit plate; 504, limit round groove; 505, limit seat; 6, uncapping assembly; 601, push cylinder; 602, uncapping seat; 603, uncapping groove; 7, liquid injection assembly; 701, liquid injection pipe; 8, adding assembly; 801, adding pipe; 9, empty groove; 10, linear guide rail two; 11, connecting plate; 12, mechanical hand; 13, extraction pipe; 14, storage rack; 15, test tube. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all the other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model. Embodiment 1

[0027] Please refer to Figure 1 - Figure 5The embodiment 1 illustrates a purification device based on QuEChERS purification technology for detection, which comprises a workbench 1, a linear guide rail 1 is fixedly installed on the top of the workbench 1, a mounting plate 3 is matched and arranged above the linear guide rail 1, support lug plates 4 are fixedly connected to the left and right ends of the bottom of the mounting plate 3, the mounting plate 3 is fixedly connected to the workbench 1 through the support lug plates 4, a mixing and separating mechanism 5 is matched and slidably connected to the linear guide rail 1, the mounting plate 3 is sequentially connected from left to right with an uncapping assembly 6, a liquid injection assembly 7 and an adding assembly 8, a hollow groove 9 is formed in the mounting plate 3 on the right side of the adding assembly 8, linear guide rails 2 are fixedly installed at the front and back sides of the edge of the hollow groove 9, a connecting plate 11 is matched and slidably connected to the linear guide rails 2, a mechanical hand 12 is matched and fixedly installed at the center position of the bottom of the connecting plate 11, an extraction tube 13 is matched and connected to the output end of the bottom of the mechanical hand 12, a rack 14 is arranged on the right side of the mixing and separating mechanism 5, the rack 14 is matched and slidably connected to the linear guide rail 1, and a test tube 15 is inserted into the rack 14;

[0028] The mixing and separating mechanism 5 comprises a bottom plate 501, a centrifugal separation assembly 502 and an extraction and mixing assembly 503, the bottom plate 501 is matched and slidably connected to the linear guide rail 1, the centrifugal separation assembly 502 is matched and slidably connected to the center position of the top of the bottom plate 501, and the extraction and mixing assembly 503 is matched and connected to the top of the centrifugal separation assembly 502.

[0029] Please refer to Figure 1 and Figure 2 , the mixing and separating mechanism 5 in the illustration moves through the linear guide rail 1 and corresponds to the uncapping assembly 6, the liquid injection assembly 7 and the adding assembly 8, the mechanical hand 12 moves through the linear guide rail 2 and corresponds to the mixing and separating mechanism 5 and the rack 14.

[0030] In this embodiment, the detection sample is first placed in the sampling tube, the sampling tube with the detection sample is inserted and limited on the extraction mixing assembly 503 in the mixing and separating mechanism 5, the mixing and separating mechanism 5 is driven to move by the linear guide rail one 2, the sampling tube is moved to the uncovering assembly 6, the cover of the sampling tube is opened under the control of the uncovering assembly 6, then the mixing and separating mechanism 5 continues to move right on the linear guide rail one 2, the sampling tube is moved to the right below the liquid injection assembly 7, the liquid injection pipe 701 injects the required reagent into the sampling tube, after the liquid injection is completed, the sampling tube is moved to the right below the adding assembly 8, the specific extractant is added into the sampling tube by the adding pipe 801, then the extraction mixing assembly 503 starts to work, the mixing cylinder 5031 drives the piston rod 5032 to move up and down, so that the sample and the extractant are fully mixed, after the mixing is completed, the centrifugal motor 5022 starts to work, the centrifugal separation is carried out to remove the impurities in the sample, after the centrifugal separation is completed, the sampling tube is moved to the right below the mechanical hand 12, the upper clear liquid is sucked by the mechanical hand 12 to control the extraction pipe 13, and the subsequent detection analysis is carried out, so that the pretreatment work of the sample can be efficiently and accurately completed, and reliable data support is provided for the residual detection.

[0031] It should be noted that during the purification process, the cover of the sampling tube can be opened or closed by the uncovering assembly 6 according to actual needs, so as to ensure the safety and sealing of the sample during the treatment process, in the centrifugal separation assembly 502, the limiting block 5023 is arranged to ensure the stability and safety of the centrifugal process, and prevent abnormal vibration and sample overflow during centrifugal separation, and the limiting plate 5034 of the extraction mixing assembly 503 ensures the accuracy and repeatability of the piston rod 5032 during the up and down movement, so as to ensure the uniformity of the mixing of the sample and the extractant, thereby improving the work efficiency and ensuring the reliability of the detection result, and meeting the requirements of modern residual detection for rapidness, high efficiency and accuracy. Example 2

[0032] Please refer to Figure 3 and Figure 4 It is illustrated that the centrifugal separation assembly 502 includes a support top plate 5021, a centrifugal motor 5022 and a limiting block 5023, the bottom of the support top plate 5021 is matched and connected with the centrifugal motor 5022, the bottom of the centrifugal motor 5022 is fixedly installed on the top of the bottom plate 501, and the support top plate 5021 is provided with a plurality of limiting blocks 5023 around the centrifugal motor 5022.

[0033] Further, the top of the bottom plate 501 is provided with a limiting circular groove 504 corresponding to the limiting block 5023, the limiting block 5023 is slidably connected in the limiting circular groove 504, and the top of the support top plate 5021 is fixedly connected with a plurality of limiting seats 505 at equal intervals in the circumference;

[0034] Further, the extraction mixing assembly 503 comprises a mixing cylinder 5031, a piston rod 5032, a placing plate 5033 and a limiting plate 5034, the bottom of the mixing cylinder 5031 is fixedly connected at the center position of the top of the supporting top plate 5021, the top of the mixing cylinder 5031 is movably connected with the piston rod 5032, the top end of the piston rod 5032 is fixedly connected with the placing plate 5033 for placing the sample tube, and the outer wall of the piston rod 5032 is fixedly connected with the limiting plate 5034;

[0035] In this embodiment, through the driving of the mixing cylinder 5031, the piston rod 5032 moves up and down in the mixing cylinder, driving the sample tube on the placing plate 5033 to reciprocate, realizing the sufficient mixing of the sample and the extraction agent, and the limiting plate 5034 ensures the stability and repeatability of the piston rod 5032 in the movement process, thereby ensuring the consistency of the mixing effect. After the mixing is completed, the centrifugal motor 5022 is started to perform centrifugal separation to remove impurities in the sample. The limiting block 5023 in the centrifugal separation assembly 502 ensures the stability and safety of the centrifugal process, preventing abnormal vibration and sample overflow during centrifugation. Through these steps, the pretreatment work of the sample is efficiently and accurately completed, providing reliable data support for residue detection.

[0036] It should be noted that the mixing cylinder 5031 can realize the rapid reciprocating motion of the piston rod, thereby improving the mixing efficiency. At the same time, the setting of the limiting plate 5034 ensures the accuracy and repeatability of the piston rod in the movement process, guarantees the consistency of the mixing effect. In the centrifugal separation assembly 502, the setting of the limiting block 5023 ensures the stability and safety of the centrifugal process, preventing abnormal vibration and sample overflow during centrifugation, so that the pretreatment work of the sample is efficiently and accurately completed, providing reliable data support for residue detection. Example 3

[0037] Please refer to Figure 6 and Figure 7 Example 3 is described, and the embodiment further illustrates Example 2. In the illustration, the uncovering assembly 6 comprises a pushing cylinder 601, an uncovering seat 602 and an uncovering groove 603. The pushing cylinder 601 is fixedly installed on the top of the mounting plate 3. The output end of the pushing cylinder 601 penetrates through the mounting plate 3 and is fixedly connected with the uncovering seat 602. The bottom of the uncovering seat 602 is provided with a plurality of uncovering grooves 603.

[0038] Further, the liquid injection assembly 7 comprises a plurality of liquid injection pipes 701 corresponding to the sample tubes placed on the placing plate 5033.

[0039] Further, the adding assembly 8 comprises a plurality of adding pipes 801 corresponding to the sample tubes placed on the placing plate 5033;

[0040] In the embodiment, by driving the air cylinder 601, the cover opening seat 602 moves in the cover opening groove 603 to realize the opening and closing operation of the sample tube cover, the liquid injection pipe 701 can accurately inject the required reagent into the sample tube under the control of the liquid injection assembly 7, ensuring the accuracy and consistency of the reagent adding, the adding pipe 801 adds the specific extractant to the sample tube under the control of the adding assembly 8, ensuring the accurate addition of the extractant, through the cooperative work of these assemblies, the pretreatment process of the sample is automated and standardized, greatly improving the efficiency and accuracy of the detection, the operation is simple, easy to maintain, and suitable for use in various laboratory environments.

[0041] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A purification device for detection based on QuEChERS purification technology, comprising a workbench (1), characterized in that: A linear guide rail (2) is fixedly installed on the top of the workbench (1). A mounting plate (3) is matched and installed directly above the linear guide rail (2). Supporting ear plates (4) are fixedly connected to the left and right ends of the bottom of the mounting plate (3). The mounting plate (3) is fixedly connected to the workbench (1) through the supporting ear plates (4). A mixing and separation mechanism (5) is slidably connected to the linear guide rail (2). From left to right, the mounting plate (3) is connected to an opening assembly (6), an injection assembly (7), and an adding assembly (8). The adding assembly (8) A slot (9) is provided on the mounting plate (3) on the right side. A linear guide rail (10) is fixedly installed on the front and rear edges of the slot (9). A connecting plate (11) is slidably connected to the linear guide rail (10). A robot (12) is fixedly installed at the center of the bottom of the connecting plate (11). An extraction tube (13) is connected to the output end of the robot (12). A shelf (14) is provided on the right side of the mixing and separation mechanism (5). The shelf (14) is slidably connected to the linear guide rail (2). A test tube (15) is inserted into the shelf (14). The mixing and separation mechanism (5) includes a base plate (501), a centrifugal separation component (502), and an extraction mixing component (503). The base plate (501) is slidably connected to a linear guide rail (2). The centrifugal separation component (502) is slidably connected at the center of the top of the base plate (501). The extraction mixing component (503) is connected to the top of the centrifugal separation component (502).

2. The purification device for detection based on QuEChERS purification technology according to claim 1, characterized in that: The mixing and separating mechanism (5) moves via linear guide rail one (2) to correspond to the opening assembly (6), the liquid injection assembly (7) and the adding assembly (8), and the robotic arm (12) is connected via linear guide rail two (10) to correspond to the mixing and separating mechanism (5) and the shelf (14).

3. The purification device for detection based on QuEChERS purification technology according to claim 1, characterized in that: The centrifugal separation assembly (502) includes a supporting top plate (5021), a centrifugal motor (5022), and limiting blocks (5023). The bottom of the supporting top plate (5021) is matched and connected to the centrifugal motor (5022). The bottom of the centrifugal motor (5022) is fixedly installed on the top of the base plate (501). Several limiting blocks (5023) are arranged around the bottom of the supporting top plate (5021) around the centrifugal motor (5022).

4. The purification device for detection based on QuEChERS purification technology according to claim 3, characterized in that: The top of the base plate (501) is provided with a limiting circular groove (504) corresponding to the limiting block (5023), and the limiting block (5023) is slidably connected in the limiting circular groove (504). A number of limiting seats (505) are fixedly connected at equal intervals around the top circumference of the supporting top plate (5021).

5. A purification device for detection based on QuEChERS purification technology according to claim 4, characterized in that: The extraction mixing assembly (503) includes a mixing cylinder (5031), a piston rod (5032), a placement plate (5033), and a limiting plate (5034). The bottom of the mixing cylinder (5031) is fixedly connected to the center of the top of the supporting top plate (5021). The top of the mixing cylinder (5031) is movably connected to the piston rod (5032). The top of the piston rod (5032) is fixedly connected to the placement plate (5033) for placing the sampling tube. The limiting plate (5034) is matched and fixedly connected to the outer wall of the piston rod (5032).

6. The purification device for detection based on QuEChERS purification technology according to claim 1, characterized in that: The opening assembly (6) includes a push cylinder (601), an opening seat (602), and an opening groove (603). The push cylinder (601) is fixedly installed on the top of the mounting plate (3). The output end of the push cylinder (601) passes through the mounting plate (3) and is fixedly connected to the opening seat (602). The bottom of the opening seat (602) is provided with a plurality of opening grooves (603).

7. A purification device for detection based on QuEChERS purification technology according to claim 5, characterized in that: The liquid injection assembly (7) includes several liquid injection tubes (701), and the several liquid injection tubes (701) correspond to the sampling tubes placed on the placement plate (5033).

8. A purification device for detection based on QuEChERS purification technology according to claim 5, characterized in that: The addition component (8) includes a plurality of addition tubes (801), which correspond to the sampling tubes placed on the placement plate (5033).

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