Heparin binding protein nucleic acid aptamer fluorescence assay kit

Abstract

The application discloses a heparin-binding protein nucleic acid aptamer fluorescence assay kit and belongs to the field of kits. The kit comprises a box body, a cover plate is hingedly connected to the top of the box body, a shaking mechanism is arranged on one side of the box body, the shaking mechanism comprises a fixing frame, the fixing frame is fixedly connected with the box body, a motor is fixedly connected to the inside of the fixing frame, an eccentric block is fixedly connected to the output end of the motor, a limiting mechanism is arranged in the inside of the cover plate, the eccentric block, the motor and the fixing frame are arranged, the motor is fixed through the fixing frame, the output end of the motor drives the eccentric block to rotate, the surface of the box body is knocked by the eccentric block to make the reagent tube in the inside of the box body vibrate, the liquid in the reagent tube is shaken, the heparin-binding protein nucleic acid aptamer fluorescence assay kit cannot shake the liquid in the reagent tube, and the problem that precipitates are prone to appearing in the liquid is solved, and the practicability is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of reagent kits, in particular to a heparin-binding protein nucleic acid aptamer fluorescence assay kit. BACKGROUND

[0002] In the published patent with the authorization announcement number CN221776433U, a heparin-binding protein assay kit is provided, which comprises a box body and a fixed plate. The upper end of the inner side of the box body is symmetrically connected with a mounting block, and the fixed plate is fixedly connected to the upper surface of the mounting block through an embedded block. The lower surface of the fixed plate is symmetrically connected with a receiving plate, and the lower surface of the receiving plate is provided with a receiving cavity. A limiting ring is slidingly connected in the receiving cavity through a spring, and the lower surface of the limiting ring is fixedly connected with a bottom plate through an extension plate. The upper surface of the fixed plate is provided with a boundary groove, and the receiving groove is provided on the side surface of the positioning hole. Two groups of fastening lead screws are movably connected through bearings at both ends of the receiving groove. The pulley discs at the same end of the two groups of fastening lead screws are connected through a transmission belt, and the end of the fastening lead screw away from the pulley disc is connected with a transmission rod through a bevel gear. The fixed plate, the fastening plate, the fastening lead screw, the fastening layer and the moving block cooperate to enable the reagent kit to fix reagent tubes of different sizes, thereby enhancing the practicability of the reagent kit.

[0003] In the above device, the fastening plate clamps and fixes the reagent tube by rotating the pulley disc. During long-term storage, the liquid in the reagent tube may precipitate. The existing heparin-binding protein nucleic acid aptamer fluorescence assay kit cannot shake the liquid in the reagent tube, which may cause the liquid to easily precipitate. UTILITARIAN CONTENT

[0004] In view of the deficiencies of the prior art, the present utility model provides a heparin-binding protein nucleic acid aptamer fluorescence assay kit, which overcomes the deficiencies of the prior art and aims to solve the problems in the background art.

[0005] In order to achieve the above-mentioned purpose, the following technical scheme is adopted in the present application: a heparin-binding protein nucleic acid aptamer fluorescence assay kit comprises a box body, a cover plate is hingedly connected to the top of the box body, a shaking mechanism is arranged on one side of the box body, the shaking mechanism comprises a fixed frame, the fixed frame is fixedly connected with the box body, an electric motor is fixedly connected inside the fixed frame, an eccentric block is fixedly connected to the output end of the electric motor, a limiting mechanism is arranged inside the cover plate, a fixed plate is slidingly connected inside the box body, a supporting mechanism is arranged at the connection between the fixed plate and the box body, a lighting mechanism is arranged at the bottom of the supporting mechanism, a plurality of through holes are formed in the surface of the fixed plate, the plurality of through holes are uniformly distributed on the surface of the fixed plate, a moving frame is slidingly connected inside the through holes, and a positioning mechanism is arranged inside the moving frame.

[0006] As a preferred implementation, the limiting mechanism comprises a first spring, the first spring is fixedly connected with the cover plate, the first spring is provided with a plurality of first springs which are uniformly distributed on the surface of the cover plate, the bottom of the first spring is fixedly connected with a positioning block, and the positioning block is slidingly connected with the cover plate.

[0007] By adopting the above technical scheme, the first spring supports the positioning block, the positioning block abuts against the top of the reagent tube to limit the reagent tube, and the reagent tube can be better limited.

[0008] As a preferred implementation, the supporting mechanism comprises a limiting block, the limiting block is slidingly connected with the box body, the side of the limiting block close to the box body is fixedly connected with a second spring, the side of the limiting block away from the box body is slidingly connected with a limiting frame, and the limiting frame is fixedly connected with the fixed plate.

[0009] By adopting the above technical scheme, the second spring supports the limiting block, the limiting block is inserted into the limiting frame, and the limiting frame supports the fixed plate, so that the fixed plate can be better supported.

[0010] As a preferred implementation, the surface of the box body is provided with an observation window.

[0011] By adopting the above technical scheme, the surface of the box body is provided with an observation window, and the reagent tube in the box body is observed through the observation window, so that the reagent tube in the box body can be better observed.

[0012] As a preferred implementation, the lighting mechanism comprises a lamp strip, the lamp strip is fixedly connected with the box body, the surface of the box body is fixedly connected with a controller, and the controller is electrically connected with the lamp strip.

[0013] By adopting the above technical scheme, the controller controls the on-off of the lamp strip, and the inside of the box body is illuminated by the lamp strip, so that the reagent tube in the box body can be better observed.

[0014] As a preferred implementation, the positioning mechanism comprises a mounting frame, the mounting frame is fixedly connected with a moving frame, the inside of the mounting frame is slidingly connected with a limiting rod, the outside of the limiting rod is sleeved with a third spring, the bottom of the limiting rod is fixedly connected with a moving block, the outside of the moving block is provided with a supporting rod, and the supporting rod is slidingly connected with the moving frame.

[0015] By adopting the technical scheme, the mobile frame is clamped and fixed to the reagent tube by abutting against the reagent tube, the limiting rod is limited by the mounting frame, the limiting rod is supported by the third spring, the supporting rod is separated from the inside of the mobile frame by abutting against the surface of the supporting rod by the moving block, and the mobile frame is positioned by abutting against the fixed plate, so that different sizes of reagent tubes can be clamped and fixed better.

[0016] As a preferred implementation form, the side of the supporting rod away from the moving block is fixedly connected with an anti-skid block, and the anti-skid block is semicircular.

[0017] By adopting the technical scheme, the friction between the supporting rod and the fixed plate is strengthened by the anti-skid block, so that the friction between the supporting rod and the fixed plate can be better strengthened.

[0018] As a preferred implementation form, the surface of the box body is fixedly connected with a rock wool thermal insulation layer.

[0019] By adopting the technical scheme, the surface of the box body is fixedly connected with a rock wool thermal insulation layer, and the heat insulation of the box body is strengthened by the rock wool thermal insulation layer, so that the heat insulation of the box body can be better strengthened.

[0020] The beneficial effects of the present application are:

[0021] 1. The heparin binding protein nucleic acid aptamer fluorescence assay kit sets an eccentric block, a motor and a fixing frame, fixes the motor through the fixing frame, drives the eccentric block to rotate through the output end of the motor, and shakes the reagent tube in the box body by knocking the surface of the box body with the eccentric block, so that the liquid in the reagent tube is evenly shaken, the problem that the existing heparin binding protein nucleic acid aptamer fluorescence assay kit cannot shake the liquid in the reagent tube and causes the liquid to easily generate precipitates is avoided, and the practicability is improved.

[0022] 2. The heparin binding protein nucleic acid aptamer fluorescence assay kit sets a positioning block and a first spring, supports the positioning block through the first spring, and limits the reagent tube through the positioning block abutting against the top of the reagent tube, so that the problem that the existing heparin binding protein nucleic acid aptamer fluorescence assay kit cannot limit the reagent tube is avoided, and the practicability is improved. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a front structure schematic view of the present application;

[0024] Figure 2 It is a cover plate structure schematic view of the present application;

[0025] Figure 3 It is a supporting mechanism structure schematic view of the present application;

[0026] Figure 4The positioning mechanism structure schematic diagram of the application.

[0027] Figure label: 1, box body; 2, shaking mechanism; 21, eccentric block; 22, motor; 23, fixed frame; 3, limiting mechanism; 31, positioning block; 32, first spring; 4, supporting mechanism; 41, second spring; 42, limiting block; 43, limiting frame; 5, surface illuminating mechanism; 51, controller; 52, light bar; 6, positioning mechanism; 61, limiting rod; 62, mounting frame; 63, third spring; 64, moving block; 65, supporting rod; 7, moving frame; 8, anti-skid block; 9, cover plate; 10, observation window; 11, fixed plate; 12, through hole. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application.

[0029] Referring to Figures 1-2 The heparin binding protein nucleic acid aptamer fluorescence assay kit comprises a box body 1, the top of the box body 1 is hingedly connected with a cover plate 9, one side of the box body 1 is provided with a shaking mechanism 2, the shaking mechanism 2 comprises a fixed frame 23, the fixed frame 23 is fixedly connected with the box body 1, the inside of the fixed frame 23 is fixedly connected with a motor 22, the output end of the motor 22 is fixedly connected with an eccentric block 21, the inside of the cover plate 9 is provided with a limiting mechanism 3, the limiting mechanism 3 comprises a first spring 32, the first spring 32 is fixedly connected with the cover plate 9, a plurality of first springs 32 are uniformly distributed on the surface of the cover plate 9, the bottom of the first spring 32 is fixedly connected with a positioning block 31, and the positioning block 31 is slidably connected with the cover plate 9; the positioning block 31 is supported by the first spring 32, and then the reagent tube is limited by the positioning block 31 abutting against the top of the reagent tube, so that the reagent tube can be better limited.

[0030] Referring to Figures 1-3 The inside of the box body 1 is slidably connected with a fixed plate 11, the connection between the fixed plate 11 and the box body 1 is provided with a supporting mechanism 4, the supporting mechanism 4 comprises a limiting block 42, the limiting block 42 is slidably connected with the box body 1, the side of the limiting block 42 close to the box body 1 is fixedly connected with a second spring 41, the side of the limiting block 42 away from the box body 1 is slidably connected with a limiting frame 43, and the limiting frame 43 is fixedly connected with the fixed plate 11; the limiting block 42 is supported by the second spring 41, then the limiting block 42 is inserted into the limiting frame 43, and then the fixed plate 11 is supported by the limiting frame 43, so that the fixed plate 11 can be better supported.

[0031] Referring to Figure 1The surface of the box body 1 is provided with an observation window 10; through the observation window 10, the reagent tube inside the box body 1 can be observed, and the reagent tube inside the box body 1 can be observed better.

[0032] With reference to Figure 3 The bottom of the supporting mechanism 4 is provided with a lighting mechanism 5, the lighting mechanism 5 comprises a light bar 52, the light bar 52 is fixedly connected with the box body 1, the surface of the box body 1 is fixedly connected with a controller 51, and the controller 51 is electrically connected with the light bar 52; the opening and closing of the light bar 52 is controlled through the controller 51, and the inside of the box body 1 is lighted through the light bar 52, so that the reagent tube inside the box body 1 can be observed better.

[0033] With reference to Figures 1-4 The surface of the fixed plate 11 is provided with a plurality of through holes 12, the plurality of through holes 12 are uniformly distributed on the surface of the fixed plate 11, the inside of the through hole 12 is slidably connected with a moving frame 7, the inside of the moving frame 7 is provided with a positioning mechanism 6, the positioning mechanism 6 comprises a mounting frame 62, the mounting frame 62 is fixedly connected with the moving frame 7, the inside of the mounting frame 62 is slidably connected with a limiting rod 61, the outside of the limiting rod 61 is sleeved with a third spring 63, the bottom of the limiting rod 61 is fixedly connected with a moving block 64, the outside of the moving block 64 is provided with a supporting rod 65, and the supporting rod 65 is slidably connected with the moving frame 7; the reagent tube is clamped and fixed by pulling the moving frame 7 to make the moving frame 7 abut against the reagent tube, the limiting rod 61 is limited by the mounting frame 62, the limiting rod 61 is supported by the third spring 63, the surface of the supporting rod 65 is abutted by the moving block 64 to make the supporting rod 65 separate from the inside of the moving frame 7, and the moving frame 7 is positioned by abutting against the fixed plate 11, so that the reagent tubes of different sizes can be clamped and fixed better.

[0034] With reference to Figure 4 The side, away from the moving block 64, of the supporting rod 65 is fixedly connected with an anti-skid block 8, and the anti-skid block 8 is semicircular; the friction between the supporting rod 65 and the fixed plate 11 is enhanced through the anti-skid block 8, so that the friction between the supporting rod 65 and the fixed plate 11 can be better enhanced.

[0035] With reference to Figures 1-3 The surface of the box body 1 is fixedly connected with a rock wool heat insulation layer; the heat insulation property of the box body 1 is enhanced through the rock wool heat insulation layer fixedly connected with the surface of the box body 1, so that the heat insulation property of the box body 1 can be better enhanced.

[0036] Working principle: the reagent tube is placed into the through hole in the fixed plate 11, then the moving frame 7 is pulled to make the moving frame 7 abut against the reagent tube to clamp and fix the reagent tube, then the mounting frame 62 limits the limiting rod 61, then the third spring 63 supports the limiting rod 61, then the moving block 64 abuts against the surface of the supporting rod 65 to make the supporting rod 65 out of the inside of the moving frame 7, the fixed plate 11 positions the moving frame 7, then the second spring 41 supports the limiting block 42, then the limiting block 42 is inserted into the limiting frame 43, then the limiting frame 43 supports the fixed plate 11, then the first spring 32 supports the positioning block 31, then the positioning block 31 abuts against the top of the reagent tube to limit the reagent tube, then the fixed frame 23 fixes the motor 22, then the motor 22 rotates the eccentric block 21 through the output end, then the eccentric block 21 knocks the surface of the box body 1 to make the reagent tube in the box body 1 vibrate, the liquid in the reagent tube is shaken, then the controller 51 controls the switch of the light bar 52, then the light bar 52 illuminates the inside of the box body 1, then the surface of the box body 1 is provided with the observation window 10, then the reagent tube in the box body 1 is observed through the observation window 10.

[0037] In the description of the utility model, it needs to explain, the term "upper" "lower" "inner" "outer" "front end" "rear end" "both ends" "one end" "the other end" and so on indicate the position relation or location relation is based on the position relation or location relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and cannot be understood as indicating or implying that the device or element must have a particular orientation, a particular orientation and operation, therefore cannot be understood as limiting the utility model. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0038] In the description of the utility model, it needs to explain, unless otherwise specified and limited, the terms "installation", "provided with", "connection" and the like should be understood broadly, for example, "connection", can be fixed connection, or detachable connection, or integral connection, can be mechanical connection, or electrical connection, can be directly connected, or indirectly connected through an intermediate medium, can be the communication inside two elements. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific situation.

[0039] The utility model has been described above in combination with specific implementation manners, but those skilled in the art should know that these descriptions are all exemplary, and do not limit the protection scope of the utility model. Those skilled in the art can make various modifications and changes to the utility model according to the spirit and principle of the utility model, and these modifications and changes are also within the scope of the utility model.

Claims

1. A heparin binding protein nucleic acid aptamer fluorescence assay kit, comprising a box body (1), characterized in that, The top of the box (1) is hingedly connected with a cover plate (9), one side of the box (1) is provided with a shaking mechanism (2), the shaking mechanism (2) comprises a fixing frame (23), the fixing frame (23) is fixedly connected with the box (1), the inside of the fixing frame (23) is fixedly connected with a motor (22), the output end of the motor (22) is fixedly connected with an eccentric block (21), the inside of the cover plate (9) is provided with a limiting mechanism (3), the inside of the box (1) is slidably connected with a fixed plate (11), the connecting part of the fixed plate (11) and the box (1) is provided with a supporting mechanism (4), the bottom of the supporting mechanism (4) is provided with a surface illuminating mechanism (5), the surface of the fixed plate (11) is provided with a plurality of through holes (12), a plurality of through holes (12) are evenly distributed on the surface of the fixed plate (11), the inside of the through hole (12) is slidably connected with a moving frame (7), and the inside of the moving frame (7) is provided with a positioning mechanism (6).

2. The heparin-binding protein nucleic acid aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that The limiting mechanism (3) comprises a first spring (32), the first spring (32) is fixedly connected with the cover plate (9), a plurality of first springs (32) are evenly distributed on the surface of the cover plate (9), and the bottom of the first spring (32) is fixedly connected with a positioning block (31).

3. The heparin-binding protein aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that, The supporting mechanism (4) comprises a limiting block (42), the limiting block (42) is slidably connected with the box (1), the side, close to the box (1), of the limiting block (42) is fixedly connected with a second spring (41), and the side, away from the box (1), of the limiting block (42) is slidably connected with a limiting frame (43).

4. The heparin-binding protein aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that The surface of the box (1) is provided with an observation window (10).

5. The heparin-binding protein nucleic acid aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that The surface of the box (1) is provided with an observation window (10).

6. The heparin-binding protein nucleic acid aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that The surface of the box (1) is provided with an observation window (10).

7. The heparin-binding protein nucleic acid aptamer fluorescence assay kit according to claim 6, c h a r a c t e r i z e d in that The positioning mechanism (6) comprises a mounting frame (62), the mounting frame (62) is fixedly connected with the moving frame (7), the inside of the mounting frame (62) is slidably connected with a limiting rod (61), the outside of the limiting rod (61) is sleeved with a third spring (63), the bottom of the limiting rod (61) is fixedly connected with a moving block (64), the outside of the moving block (64) is provided with a supporting rod (65), and the supporting rod (65) is slidably connected with the moving frame (7).

8. The heparin-binding protein nucleic acid aptamer fluorescence assay kit according to claim 1, c h a r a c t e r i z e d in that, The side, away from the moving block (64), of the supporting rod (65) is fixedly connected with an anti-skid block (8), and the anti-skid block (8) is semicircular. The surface of the box (1) is fixedly connected with a rock wool heat insulation layer.

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